JPH064018B2 - Bacterial transplant device - Google Patents

Description

TECHNICAL FIELD The present invention relates to a bacterium transplantation device for transplanting the bacterium in a cultured petri dish to another petri dish.

<Prior Art> Conventionally, when transplanting cultured bacteria to another petri dish, most of the work has conventionally been done manually by using a transplant needle, but when the amount becomes large, the burden becomes very heavy and continuous work is difficult. There was a drawback that became. When performing this transplanting operation using a machine, especially when inoculating the medium surface of the transplanting petri dish with bacteria caught from the original petri dish, it is difficult to control the depth of the transplantation needle, and the medium of the petri dish for transplanting is difficult to control. There was a disadvantage that the culture medium surface was roughened by piercing the surface with a transplant needle and moving the dish while the needle was pierced, so that uniform culture could not be performed.

<Purpose> An object of the present invention is to solve the above-mentioned drawbacks of the prior art, and to provide a bacterium transplanting device capable of easily bacterium transplantation without roughening the culture medium surface of an inoculating petri dish.

<Structure> The present invention is an apparatus for transplanting bacteria that catches bacteria from a cultured petri dish with a transplanting needle and contacts the petri dish for transplantation. And a rotor for sequentially setting a plurality of radially mounted pieces on the circumference in a downward position by rotating the transplantation needle, and the transplantation needle is detachably clamped by a chuck mechanism and inoculated into a petri dish for transplantation. In this case, the tip of the transplanted needle in the sandwiched state is lowered to a position close to the culture medium surface of the petri dish for transplantation by lowering the lifting table, and then the sandwiching of the transplanted needle is released to allow the culture medium to fall naturally. It is a bacterium transplantation device having a control mechanism for making it reach a surface.

<Example> FIG. 1 is an overall perspective view of a bacteria transplanting apparatus showing an example, FIG.
The figure is a cross-sectional view of the chuck mechanism, FIG. 3 is a cross-sectional view for explaining the function of the chuck function, FIGS. 4 (A), (B), and (C) are views showing examples of transplant needles, and FIG. 9 is a flowchart of a transplanting operation by the apparatus of the embodiment.

First, a description will be given with reference to FIG. Rotor 62 on base 61
Is provided, and the base 61 itself moves up and down along the lifting shaft 63 in the vertical direction (Z-axis direction). The rotor 62 is rotatable around a horizontal shaft 64. Then, a plurality of graft needles 1 are radially mounted on the circumference of the rotor 62. The rotor 62 is rotated by a predetermined rotation angle by the drive source 65 and the speed reducer 66 to sequentially move the new graft needle 1 to the downward set position. On the other hand, an XY table 71 on which a pair of the cultured petri dish G and the transplanted petri dish I can be fixedly mounted.
Is provided so that it can be set at a predetermined XY position along the guide shafts 72, 73 by a drive source and its transmission mechanism (not shown). The raising and lowering of the base 61, the rotation of the rotor 62, and the movement of the XY table 71 are performed at a constant timing by a control mechanism (not shown).

The transplant needle 1 is attached to the rotor 62 by the chuck portion 2. The chuck mechanism of the chuck unit 2 will be described with reference to FIGS. 2 and 3.

The chuck part 2 includes a chuck 21, an outer cylinder part 22, and a guide cylinder 2.
3, a leaf spring 24, an electromagnetic coil 25, etc., and the chuck 21 plays a role of holding the graft needle 1 from the surroundings. The chuck 21 is rotatably supported by a pin 26 and is constantly opened by a spring (not shown). Is urged by. The outer cylinder portion 22 is held by a guide pin 27 so as to be movable in the vertical direction, and when the outer cylinder portion 22 moves downward, the chuck 21 is squeezed against the spring biasing force. The needle 1 is clamped. The leaf spring 24 and the electromagnetic coil 25 are connected to the outer cylinder 2
It is a means for moving 2 up and down. The electromagnetic coil 25 pulls the outer cylinder portion 23 upward against the leaf spring 24 in the ON state. On the other hand, when the electromagnetic coil 25 is turned off, the leaf spring 2
4 pushes the outer tube portion 23 downward. The guide cylinder 23 controls the posture of the transplant needle 1. That is, by inserting the graft needle 1 into the guide cylinder 23, the graft needle 1
Is regulated in a straight line in the direction of the axis S, and the movement of the graft needle 1 is prevented.

When inoculating the transplanted petri dish with the caught bacteria, first, the transplantation needle 1 is lowered to a position close to the agar K of the petri dish I for transplantation using the chuck mechanism. This lowering is performed by lowering the base 61. Then, the chuck mechanism is made free to drop the graft needle 1 freely. Then, since the impact force of the transplant needle 1 is not so large and the agar K has elasticity, the transplant needle 1 stays on the surface of the agar K without piercing the agar K. This allows inoculation without roughening the surface of the medium.

The tip 11 of the transplant needle 1 is formed round. Another example of the graft needle 1 is shown in FIG. In the example shown in (A), a nichrome wire or heat-resistant steel having a rounded tip 11 and a conductive material 1b such as copper connected to another electric resistor material 1a are transplanted so that they can be sterilized by heat. It is a needle. Also (B)
The implanting needle 1 is a metal rod having a rounded tip by bending the tip 11. In (C), the graft needle 1 has a rounded tip and a thin intermediate portion 12 to reduce the thermal conductivity so that it can be sterilized with a small amount of heat at the time of sterilization.

Next, the transplantation operation of bacteria by this device will be described with reference to FIG.

First, the rotor 62 rotates, the new graft needle 1 is set in the downward position, the XY table 71 moves, and the position of a predetermined colony of the original petri dish G comes under the graft needle 1. Then, the base 61 descends along the elevating shaft 63 and then rises. As a result, the transplant needle 1 pierces the medium surface of the original petri dish G, and then rises to carry out fishing. Next, the XY table 71 is moved so that a predetermined position of the petri dish I for transplantation is set right below the transplantation needle 1. Then again base 6
1 is lowered, and the tip 11 of the transplant needle 1 is lowered to a position close to the medium surface of the petri dish I for transplant. Then, at that position, the chuck mechanism is turned off, and the graft needle 1 is dropped. Since the drop distance is not large, the elasticity of the medium surface absorbs the force, and the needle 1 stays on the medium surface without being pierced. Then, in the case of linear implantation, the XY table 71 is moved by a predetermined distance. Further, when transplanting in a dot shape, it is not necessary to move the table 71. When the inoculation is finished, the base 61 is lowered by the distance that the graft needle 1 was dropped, the grip needle 21 holds the graft needle 1 again, and then the base 61 is raised. After the transplanting operation as described above is performed a predetermined number of times between the petri dishes G and I, the transplanting operation is completed.

<Effect> The present invention has the above-mentioned constitution, and when transplanting a fungus from a cultured petri dish to a new petri dish, surely transplanting to the surface of the medium surface without piercing the transplanting needle into the surface of the medium to be inoculated. The needle can be reached. Therefore, a good transplantation state can be secured without roughening the medium surface. In addition, since a plurality of transplant needles are attached on the circumference of the rotor and these are sequentially moved to the downward set position, the transplant operation can be performed very smoothly.

[Brief description of drawings]

FIG. 1 is an overall perspective view of a bacteria transplanting device showing an embodiment, FIG.
FIG. 4 is a sectional view of the chuck mechanism, FIG. 3 is a sectional view for explaining the operation of the chuck mechanism, FIGS. 4 (A), (B), and (C) are diagrams showing examples of the implantation needle, and FIG. 9 is a flowchart of a transplanting operation by the apparatus of the embodiment. DESCRIPTION OF SYMBOLS 1 ... Implanting needle 2 ... Chuck part 21 ... Chuck 22 ... Outer cylinder part 23 ... Guide cylinder 24 ... Leaf spring 25 ... Electromagnetic coil 61 ... Base 62 ... Rotor 63 ... Elevating shaft 71 ... XY table 72, 73 ... Guide Axis G ... Petri dish I ... Transplant dish

Claims (1)

[Claims] 1. A transplantation device for bacteria, which is used to inoculate a petri dish for cultivation, which is cultivated from a cultured petri dish with a transplanting needle, and which is vertically movable and attached to the elevator. A rotor for sequentially setting a plurality of graft needles radially mounted on the circumference in a downward position by rotation, and the graft needles are detachably clamped by a chuck mechanism and used to inoculate a petri dish for transplantation. In addition, by lowering the elevation table to lower the tip of the transplanted needle in the sandwiched state to a position close to the medium surface of the petri dish for transplantation, unclamp the transplanted needle and let it fall naturally, A device for transplanting a bacterium, which is provided with a control mechanism for making it reach.