JPH0649654B2 - Highly stable enteric useful bacterial preparation and method for producing the same - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION <Industrial field of application> The present invention relates to a highly stable enteric useful bacterial preparation which can be used in the fields of food, feed, medicine and the like, and a method for producing the same.

<Prior Art> Useful enteric bacteria such as lactic acid bacteria suppress other bacteria, especially crumb-negative bacteria such as Escherichia coli, by keeping the pH in the intestine and keeping the acidity, and enterobacteria of these bacteria It also has the effect of preventing bacterial infection by suppressing colonization and proliferation of the bacteria.

Recently, by utilizing the action of the intestinal useful bacteria, it has been applied to health foods or intestinal preparations, etc., when humans and animals eat, because the pH of the gastric juice is low, the intestinal useful bacteria are The problem is that most of them die before they reach the intestine.
Therefore, a method of protecting the intestinal useful bacteria from external factors and stabilizing it, for example, a method of dispersing viable cells in an aqueous layer in a W / O / W type emulsion in JP-A-62-220186. In addition, JP-A-62-201823
JP-A-60-172280 discloses a method of encapsulating a fat / oil in which live cells are dispersed using a double or triple nozzle in a gelatin soft capsule, as disclosed in JP-A-60-172280. Or JP-A-60-1
Japanese Patent No. 88060 proposes a method of freeze-drying after adding a stabilizer.

However, with the method using a W / O / W type emulsion, it is difficult to make a powder when formulating, and even with the method using a nozzle, the particle size becomes large,
There is a problem in blending with other formulations. Further, since the content of useful enteric bacteria as a core substance is low, it is necessary to use many preparations, which is economically disadvantageous. Furthermore, it is desirable to use as the coating agent an oil or fat that is not decomposed in the stomach but is decomposed in the intestine, but in order to take out the preparation as a powder at room temperature, it is necessary to have a melting point of 40 ° C. or higher. Generally, it is difficult for the useful bacteria in the intestine to survive. Therefore, it is desired to develop a useful enteric bacterial preparation that can use oils and fats as a coating agent and a method for producing the same.

<Problems to be Solved by the Invention> An object of the present invention is to provide a highly stable enteric useful bacterial preparation which is excellent in coating performance and can be effectively used in the fields of food, feed or medicine, and a method for producing the same. is there.

Another object of the present invention is to increase the content of intestinal useful bacteria, which is the core substance, and to obtain excellent coating performance with a small amount of coating agent. To provide a useful enteric bacterial preparation and a method for producing the same.

Still another object of the present invention is to provide a method for producing a highly stable enteric useful bacterial preparation which does not require melting of the coating agent and therefore avoids the consumption of a large amount of energy and is easy to maintain the device to be used. Especially.

<Means for Solving the Problems> According to the present invention, a powdery substance as a core substance containing useful enteric bacteria, and a lipid having a melting point of 40 ° C. or higher as a coating agent for coating the entire peripheral surface of the core substance. A highly stable enteric useful bacterial preparation comprising a powdery substance, which comprises coating the core substance by contacting and colliding with the core substance.

Further, according to the present invention, a powdery body containing useful enteric bacteria and a lipid powdery substance having a melting point of 40 ° C. or higher as a coating agent are mixed at a ratio (weight of powdery body containing useful enteric bacteria / coating agent). Weight) 0.
Production of a highly stable enteric useful bacterial preparation characterized by contacting and colliding with each other at 1 to 50 to adhere and coat the lipid powder on the entire peripheral surface of the powder containing the enteric useful bacteria. A method is provided.

Furthermore, according to the present invention, a highly stable intestinal useful bacterial preparation obtained by contacting and colliding the core substance with the coating agent, is further contacted with and collided with a hydrophilic substance to coat the core substance with high stability. An enteric useful bacterial preparation is provided.

Furthermore, according to the present invention, a powdery body containing useful enteric bacteria and a lipid powdery substance having a melting point of 40 ° C. or higher as a coating agent are mixed at a ratio (weight of powdery body containing useful enteric bacteria / coating agent). weight)
0.1 to 50 contact / collision, the whole peripheral surface of the powdery substance containing the intestinal useful bacteria is adhered / coated to the lipid powdery substance, and then a hydrophilic substance is contacted / collided, There is provided a method for producing a highly stable enteric useful bacterial preparation, which comprises further adhering and coating the above-mentioned hydrophilic substance on a powder containing enteric useful bacteria.

The present invention will be described in more detail below.

Examples of useful enteric bacteria as the core substance that can be used in the present invention include B. bifidum, B. breve, B. adolescentis,
B.infantis, B.longum, etc., genus Bifidobacterium, S.faecal
genus storeptococcus such as is, S. thermophilus, L. acidophil
Known intestinal useful bacteria such as Lactobcillus genus such as us, L. bulgaricus, L. casei and the like can be preferably mentioned. When used, commercially available products can be used, and two or more of the intestinal useful bacteria can be used in combination. In addition, the intestinal useful bacteria can be used as they are, for example, amino acids such as lactose, lysine, aspartic acid, and arginine, polymeric substances such as gelatin, albumin, peptone, or their degradation products, skim milk powder, or water-soluble vitamins. It can also be freeze-dried together with the above and powdered.

In the present invention, examples of the lipid powder having a melting point of 40 ° C. or higher used as a coating agent include beef tallow, lard, animal fats such as fish oil, soybean oil, rapeseed oil, coconut oil, cottonseed oil and the like, and vegetable oils obtained from these and the like. Hydrogenated oils, fatty acid monoglycerides, fatty acid diglycerides, propylene glycol fatty acid esters, sucrose fatty acid esters, fatty acids, higher alcohols, waxes, tocopherols, phospholipids containing phosphorus and nitrogen, glycolipids having sugar as a constituent, sulfone It can be selected from one or more selected from the group consisting of sulfolipids having an acid group, sterols, hydrocarbons and cured products thereof.

The mixing ratio of the core substance containing the enteric useful bacteria used in the present invention and the coating agent (the weight of the powdery substance containing the enteric useful bacteria / the coating agent weight) is 0.1 to 50, and the range is within the range. Outside, no fully coated enteric beneficial bacterial preparations can be obtained.

In the present invention, the coating agent may be further coated with a hydrophilic substance in order to improve the dispersibility in the aqueous system. The hydrophilic substance may be selected from one or two or more members selected from the group consisting of starch such as corn and potato, hydrophilic polymer such as dextrin, oligosaccharide and protein, and degradation products thereof. The mixing ratio of the hydrophilic substance is 1 to 2 with respect to the whole enteric useful bacterial preparation of the present invention.
It is preferably added in the range of 0% by weight.

In order to produce the intestinal useful bacterial preparation of the present invention, the powder substance which is the core substance and the powder substance which is a coating material are, for example, a ball mill, an electric mortar, a highly efficient powder mixing device, and a high-speed air stream. By contacting and colliding the powdery substances with each other by convection or the like, the coating agent can be attached and coated on the entire surface of the powdery substance of the core substance. Further, in order to improve the dispersibility in addition to the water system, when the hydrophilic substance is further attached / coated, the coating agent may be further coated with the hydrophilic substance by contacting / colliding with the same method. You can At this time, if contact / collision is performed under extremely extreme conditions,
Since the temperature of the powdery substance may rise and useful bacteria in the intestine may be killed, it is preferable to perform the treatment under mild conditions as much as possible.
That is, it is sufficient that the temperature of the powdery body at the time of contact / collision is less than 40 ° C. To further improve the coating performance,
It is desirable to previously mix the powdery substance that is the core substance and the coating agent, and it is particularly preferable to perform the contact and collision in several times.

<Effects of the Invention> In the highly stable enteric useful bacterial preparation of the present invention and the method for producing the same, the coating performance can be significantly improved as compared with conventionally known preparations, and enteric useful bacteria are present in the stomach. It is useful in the field of food, feed or medicine because it can be effectively prevented from dying. Further, the content of useful enteric bacteria as a core substance can be increased, and excellent coating performance can be obtained with a small amount of coating agent, which is very economically advantageous. Further, in the production method of the present invention, since it is not necessary to melt the coating material, energy consumption can be avoided, and maintenance and management of the device used is easy.

<Examples> The present invention will be described in more detail with reference to Examples and Comparative Examples, but the present invention is not limited thereto.

Example 1 Lactic acid bacteria (S. Faecalis) (1.25 × 10 ¹⁰ cells / g) 70
After adding 300 g of hardened beef tallow oil powder to 0 g and mixing,
The mixture was mixed for 3 minutes using a Nara hybridization system (manufactured by Nara Machinery Co., Ltd.). 842 g of the obtained powder was subjected to a storage test for 3 months at a temperature of 35 ° C. and a humidity of 65% to measure the decrease of intestinal useful bacteria. The results are shown in Table 1.

Comparative Example 1 The lactic acid bacterium (S. Faecalis) used in Example 1 (1.25 × 1)
A storage test was conducted in the same manner as in Example 1 using 700 g of ( ¹⁰ cells / g). The results are shown in Table 1.

From the above, the formulation according to the present invention showed only a slight reduction in the bacteria during storage for 3 months.

Example 2 Lactobacillus (B. longum) (1.76 × 10 ¹⁰ cells / g) 354
150 g of rapeseed hydrogenated oil fine powder was added to g, and mixed with a Hensyl mixer at 1000 rpm for 16 minutes, and then 154 g of rapeseed hydrogenated oil fine powder was further added and mixed for 4 minutes. Then the obtained powder is further added to cornstarch 50
g was added, and the same mixing treatment was performed for 3 minutes to obtain 668 g of powder. The obtained powder was suspended in the Japanese Pharmacopoeia disintegration test method 1st solution (pH 1.2), stirred at 37 ° C. for 2 hours, adjusted to pH 6.5, and viable against the number of bifidobacteria. When the acid resistance test was conducted by measuring the ratio of the bacteria, the survival rate was 64.2%.

Comparative Example 2 The lactic acid bacterium (B. longum) used in Example 2 (1.76 × 10 6)
An acid resistance test was conducted in the same manner as in Example 2 using 354 g ( ¹⁰ pieces / g), and the survival rate was 0.4%.

Example 3 L. acidophilus (1.60 × 10 ¹⁰ cells / g)
480 g, stearic acid monoglyceride fine powder 12
0 g was added and mixed. Next, after mixing for 4 minutes with a Nara hybridization system (manufactured by Nara Machinery Co., Ltd.), 150 g of fine rice wax powder was further added,
Mixed for a minute. Next, lactose 5 was added to the obtained powder.
0g was added, and the same mixing treatment was performed for 2 minutes, 687g.
Of powder was obtained. When an acid resistance test was conducted on the obtained powder in the same manner as in Example 2, the result was 76.7%.

Comparative Example 3 L. acidophilus (1.60 used in Example 3)
When an acid resistance test was conducted in the same manner as in Example 2 using 480 g of x10 ¹⁰ cells / g), the survival rate was 0.3%.

From the above results, it was found that the intestinal useful bacterial preparation of the present invention was excellent even in the acid resistance test.

Continuation of the front page (51) Int.Cl. ⁵ Identification number Office reference number FI technical display location A61K 9/50 M 7329-4C

Claims (4)

[Claims] 1. A useful enteric bacterium comprising a powdery substance as a core substance containing enteric useful bacteria and a lipid powdery substance having a melting point of 40 ° C. or higher as a coating agent for coating the entire peripheral surface of the core substance. What is claimed is: 1. A highly stable enteric useful bacterial preparation obtained by contacting and colliding the coating material with the core substance to coat the core substance. 2. A powdery material containing useful enteric bacteria and a lipid powdery substance having a melting point of 40 ° C. or higher as a coating agent (mixing ratio (weight of powdery material containing useful enteric bacteria / weight of coating agent)) 0 A highly stable enteric useful bacterial preparation characterized by contacting and colliding at 1 to 50 to adhere and coat the lipid powder on the entire peripheral surface of the powder containing the enteric useful bacteria. Production method. 3. A powdery substance as a core substance containing intestinal useful bacteria, a lipid powdery substance having a melting point of 40 ° C. or higher as a coating agent for coating the entire peripheral surface of the core substance, and the lipidic powdery substance. Which is a useful enteric bacterial preparation containing a hydrophilic substance for coating a core substance by further contacting and colliding the hydrophilic substance with a coated preparation obtained by contacting and colliding the core substance with the coating agent. A highly stable intestinal useful bacterial preparation comprising: 4. A powdery material containing useful enteric bacteria and a lipid powdery substance having a melting point of 40 ° C. or higher as a coating agent, in a mixing ratio (weight of powdery material containing useful enteric bacteria / weight of coating agent) of 0. 1 to 50, the lipid powder is adhered and coated on the entire peripheral surface of the powder containing the intestinal useful bacteria, and the hydrophilic substance is then contacted and collided to form the intestine. A method for producing a highly stable enteric useful bacterial preparation, which comprises further adhering and coating the hydrophilic substance on a powder containing the internal useful bacteria.