JPH0661B2 - Flocculant-producing microorganism - Google Patents
Flocculant-producing microorganismInfo
- Publication number
- JPH0661B2 JPH0661B2 JP2083078A JP8307890A JPH0661B2 JP H0661 B2 JPH0661 B2 JP H0661B2 JP 2083078 A JP2083078 A JP 2083078A JP 8307890 A JP8307890 A JP 8307890A JP H0661 B2 JPH0661 B2 JP H0661B2
- Authority
- JP
- Japan
- Prior art keywords
- ferm
- flocculant
- strain
- kym
- activated sludge
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Landscapes
- Separation Of Suspended Particles By Flocculating Agents (AREA)
- Activated Sludge Processes (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
【発明の詳細な説明】 （産業上の利用分野） 本発明は凝集剤産生新規微生物に関し、更に詳しくは活
性汚泥法等による排水凝集処理方法において使用した場
合、活性汚泥のバルキングを防止して活性汚泥と処理水
との分離を効率的に行うことが出来る新規な凝集剤産生
新規微生物を提供する。TECHNICAL FIELD The present invention relates to a novel flocculant-producing microorganism, and more specifically, when used in a wastewater flocculation treatment method such as an activated sludge method, the activated sludge is prevented from bulking and activated. To provide a novel flocculant-producing novel microorganism capable of efficiently separating sludge and treated water.
（従来の技術及びその問題点） 従来、各種有機物を含む排水の処理方法として活性汚泥
方式が広く使用されている。この活性汚泥方式は効率の
高い処理方法であり、良質な処理水が経済的に得られる
ことから最も広く普及している処理方法である。(Prior art and its problems) Conventionally, an activated sludge system has been widely used as a method for treating wastewater containing various organic substances. This activated sludge system is a highly efficient treatment method, and is the most widely used treatment method because it produces high quality treated water economically.
上記活性汚泥方式において残された最も重要な問題は、
処理後の処理水と活性汚泥との分離であり、処理水と活
性汚泥とは沈澱槽である分離領域において活性汚泥が速
やかに沈降分離することが望ましいが、分離領域におい
て静置時に糸状菌等の発生によるバルキング現象やデフ
ロック現象が生じて活性汚泥の凝集フロック作用が低下
し、活性汚泥の沈降分離が不十分となり、活性汚泥の流
出という問題が生じる。The most important problem left over in the activated sludge system is
It is a separation of treated water and activated sludge after treatment, and it is desirable that the activated sludge quickly settles and separates in the separation area, which is a settling tank, but filamentous fungi, etc. when standing still in the separation area. This causes a bulking phenomenon and a deflock phenomenon due to the generation of the activated sludge to reduce the flocculating floc action of the activated sludge, resulting in insufficient sedimentation and separation of the activated sludge, which causes a problem of activated sludge outflow.
活性汚泥と処理水との分離を促進させる方法として、カ
チオンポリマー等の高分子凝集剤や多価金属イオン等の
無機凝集剤を使用する方法が知られているが、これらの
凝集剤は生物分解性が不十分である為、処理水と共に放
水されることにより環境汚染の問題が派生する。As a method for promoting the separation of activated sludge and treated water, a method of using a polymer flocculant such as a cationic polymer or an inorganic flocculant such as a polyvalent metal ion is known. Due to its insufficient property, the problem of environmental pollution is caused by being discharged together with the treated water.
従って本発明の目的は、排水凝集処理方法において、活
性汚泥のバルキング現象を生じることなく効率的に活性
汚泥を分離することが出来る凝集剤産生新規微生物を提
供することである。Therefore, an object of the present invention is to provide a flocculant-producing novel microorganism capable of efficiently separating activated sludge without causing the bulking phenomenon of activated sludge in a wastewater coagulation treatment method.
（問題点を解決する為の手段） 上記目的は以下の本発明によって達成される。(Means for Solving Problems) The above object is achieved by the present invention described below.
即ち、本発明は、アシネトバクター属アシネトバクター
ＫＹＭ−３株（FERM P-11335）、エンテロバクター属エ
ンテロバクターＫＹＭ−５株（FERM P-11337）、オーレ
オバクテリウム属オーレオバクテリウムＫＹＭ−６株
（FERM P-11357）及びオエルスコビア属オエルスコビア
ＫＹＭ−７株（FERM P-11358）からなる群から選ばれる
少なくとも１種の凝集剤産生微生物である。That is, the present invention relates to the Acinetobacter genus Acinetobacter KYM-3 strain (FERM P-11335), the Enterobacter genus Enterobacter KYM-5 strain (FERM P-11337), and the Aureobacterium genus Aureobacterium KYM-6 strain ( FERM P-11357) and Oerscovia spp. Oerscovia KYM-7 strain (FERM P-11358), which is at least one flocculant-producing microorganism.
（作 用） 活性汚泥を用いる排水凝集処理方法において、凝集物質
生産菌として新規な菌又は該菌が産生する凝集剤を使用
することによって、バルキング現象の主たる原因である
糸状菌の発生が抑制され、活性汚泥と処理水との分離が
効率的となる。更に好ましい実施態様では分離領域のｐ
Ｈを特定の範囲とすることによって活性汚泥と処理水の
分離が著しく促進される。(Operation) In a wastewater coagulation treatment method using activated sludge, the use of a novel fungus or a flocculant produced by the fungus as a flocculant-producing bacterium suppresses the generation of filamentous fungus, which is the main cause of the bulking phenomenon. , The separation of activated sludge and treated water becomes efficient. In a further preferred embodiment, the isolation region p
By setting H to a specific range, the separation of activated sludge and treated water is significantly promoted.
（好ましい実施態様） 次に好ましい実施態様を挙げて本発明を更に詳しく説明
する。(Preferred Embodiment) Next, the present invention will be described in more detail with reference to preferred embodiments.
本発明者は、公知の多数の凝集剤生産菌ロードコッカス
・エリスロポレスをフラクトース培地で培養し、その凝
集効果（力値）を測定して行く過程で、力価が通常の２
倍以上上昇する培養液が存在することを見出した。この
培養液には数種の菌が混在しており、これらの菌を分離
したところ、特に優れた力価を示す菌を発見した。In the process of culturing a large number of known flocculant-producing bacteria Rhodococcus erythropoles in a fructose medium and measuring the flocculating effect (potency value), the present inventor has a normal titer of 2
It was found that there was a culture solution that increased more than twice. Several kinds of bacteria were mixed in this culture solution, and when these bacteria were separated, a bacteria showing a particularly excellent titer was found.
上記本発明の凝集剤生産菌は、シュウドモナス属、アシ
ネトバクター属、アグロバクテリウム属、エンテロバク
ター属、オーレオバクテリウム属及びオエルスコビア属
からなる群から選ばれる少なくとも１属に属する凝集剤
生産能を有する菌であり、従来公知のロードコッカス・
エリスロポレスＫＲ−２５６−２、ＦＥＲＭ−ＰNO.３
９２３及びロードコッカス・エリスロポレスＫＲ−Ｓ−
１、ＦＥＲＭ−ＰNO.３５３０等とは異なる属に属し、
その凝集能力はこれらの公知菌の２〜３倍或いはそれ以
上である。これらの属のうち、シュウドモナス属、アシ
ネトバクター属、アグロバクテリウム属、エンテロバク
ター属、オーレオバクテリウム属及びオエルスコビア属
からなる群から選ばれる少なくとも１属を含む微生物群
を本発明ではＲ−３と称しているが、夫々既に微工研菌
寄第１１３３３号（FERM P-11333）、同第１１３３４号
（FERM P-11334）、同第１１３３５号（FERM P-1133
5）、同第１１３３６号（FERM P-11336）、同第１１３
３７号（FERM P-11337）、同第１１３５７号（FERM P-1
1357）及び同第１１３５８号（FERM P-11358）として寄
託されている。The flocculant-producing bacterium of the present invention has a flocculant-producing ability belonging to at least one genus selected from the group consisting of Pseudomonas, Acinetobacter, Agrobacterium, Enterobacter, Aureobacterium and Oerscovia. It is a fungus, and the conventionally known Rhodococcus
Erythropoles KR-256-2, FERM-PNO.3
923 and Lord Coccus erythropoles KR-S-
1, belongs to a genus different from FERM-PNO.3530,
Its aggregating ability is 2-3 times or more than those of these known bacteria. Among these genera, a microorganism group including at least one genus selected from the group consisting of Pseudomonas, Acinetobacter, Agrobacterium, Enterobacter, Aureobacteria and Oerscovia is referred to as R-3 in the present invention. As mentioned above, each of them is already institute for microbiological research, Microbiology No. 11333 (FERM P-11333), No. 11334 (FERM P-11334), No. 11335 (FERM P-1133).
5), No. 11336 (FERM P-11336), No. 113.
No. 37 (FERM P-11337), No. 11357 (FERM P-1
1357) and 11358 (FERM P-11358).
上記凝集剤生産新規微生物は、以下の菌学的性質を有し
ている。The above flocculant-producing novel microorganism has the following mycological properties.
上記第１表に示す菌学的性質について、細菌の同定書で
あるバージー・マニュアル・システマチック・バクテオ
ロジー第１、２巻（Bergey's Manualof Systematic Bac
teriology Volume 1、2）、（1984年）で検討した結果、
ＫＹＭ−１株は同書１６５頁に記載されているシュウド
モナス・フルオレセンスと一致し、ＫＹＭ−１株はシュ
ウドモナス・フルオレッセンスと同定し、微工研菌寄第
１１３３３号（FERM P-11333）として寄託されている。 Regarding the mycological properties shown in Table 1 above, Bergey's Manualof Systematic Bac
teriology Volume 1, 2), (1984),
The KYM-1 strain was identical to Pseudomonas fluorescens described on page 165 of the same book, and the KYM-1 strain was identified as Pseudomonas fluorescens, and was identified as Microindustrial Research Institute No. 11333 (FERM P-11333). Has been deposited.
ＫＹＹ−２株は同書１７５頁記載のシュウドモナス・セ
パシアと考えると妥当であり、微工研菌寄第１１３３４
号（FERM P-11334）として寄託されている。The KYY-2 strain is appropriate when considered to be Pseudomonas cepacia described on page 175 of the same document.
Deposited as Issue No. (FERM P-11334).
ＫＹＭ−３株は同書３０３頁記載のアシネトバクター属
細菌と一致し、微工研菌寄第１１３３５号（FERM P-113
35）として寄託されている。The KYM-3 strain is consistent with the bacterium of the genus Acinetobacter described on page 303 of the same book, and is referred to as Microindustrial Research Institute No. 11335 (FERM P-113
35) has been deposited.
ＫＹＭ−４株は同書２５４頁記載のアグロバクテリウム
・レイデオバクターと殆ど記載は一致するものの、糖の
資化性等細かい点で少し異なるので同種の近縁類と考え
るのが分類学的に妥当であり、本菌は微工研菌寄第１１
３３６号（FERM P-11336）として寄託されている。Although the description of KYM-4 strain is almost the same as that of Agrobacterium reideobacter described on page 254 of the same book, it is taxonomically considered to be a related species because it is slightly different in details such as sugar assimilation. This is appropriate and this microorganism is
Deposited as 336 (FERM P-11336).
ＫＹＭ−５株は同書４６５頁記載のエンテロバクター属
細菌と属レベルで完全に一致し、微工研菌寄第１１３３
７号（FERM P-11337）として寄託されている。The KYM-5 strain was completely in genus level with the Enterobacter genus bacteria described on page 465 of the same document, and was found to be Microorganisms Research Institute No. 1133.
Deposited as No. 7 (FERM P-11337).
ＫＹＭ−６株は同書１３２３頁記載のオーレオバクテリ
ウム属細菌、ＫＹＭ−７株は同書１４８９頁記載のオエ
ルスコビア属細菌と夫々属レベルの記載は一致する。The description of the KYM-6 strain is the same as that of the bacterium of the genus Aureobacterium described on page 1323 of the same book, and the description of the KYM-7 strain is the same as that of the bacterium of the genus Oerscovia described on page 1489 of the same book.
ＫＹＭ−６株は、微工研菌寄第１１３５７号（FERM P-1
1357）、ＫＹＭ−７株は、微工研菌寄第１１３５８号
（FERM P-11358）として夫々寄託されている。The KYM-6 strain is microbial strain No. 11357 (FERM P-1
1357) and KYM-7 strain have been deposited as Microindustrial Research Institute No. 11358 (FERM P-11358).
本発明の凝集剤は、以上の少なくとも１属を含む微生物
の培養物又は培養処理物を主成分とするものであって、
培養液そのもの、その濃縮物、濾液、その濾過残渣、そ
れらの乾燥物等いずれの形態でもよい。The aggregating agent of the present invention is mainly composed of a culture or a culture-treated product of a microorganism containing at least one of the above genus,
The culture solution itself, a concentrate thereof, a filtrate, a filtration residue thereof, a dried product thereof or the like may be used.
又、上記凝集剤にはカルシウムイオン等のカチオン性無
機塩を１種以上含有させたり、或いは使用時に含有させ
ることによって、それらの凝集能を更に向上させること
が出来る。In addition, the aggregating ability can be further improved by incorporating one or more cationic inorganic salts such as calcium ions into the aggregating agent or by incorporating them at the time of use.
本発明の排水凝集処理方法の特徴は、上記の凝集剤生産
菌を少なくとも１種以上使用にする点であり、適用され
る排水は懸濁物を含むいずれの排水でもよいが、特に活
性汚泥を使用する排水凝集処理方法において、活性汚泥
と処理水との分離領域においてバルキングの主たる原因
となる糸状菌の発生及びその増殖が抑えられる。従って
本発明は処理水中において糸状菌が発生し易い排水の処
理に特に有効である。The feature of the wastewater coagulation treatment method of the present invention is that at least one or more of the above flocculant-producing bacteria is used, and the applied wastewater may be any wastewater containing a suspension, but particularly activated sludge is used. In the wastewater coagulation treatment method used, generation and growth of filamentous fungi, which are the main cause of bulking, can be suppressed in the separation area between activated sludge and treated water. Therefore, the present invention is particularly effective for treating wastewater in which filamentous fungi are easily generated in treated water.
微生物を使用する活性汚泥方式による排水凝集処理方法
自体は周知であり、本発明はこれらの周知のいずれの排
水凝集処理方法においても応用出来るものであり、特に
限定されない。A method for effluent coagulation treatment by an activated sludge system using microorganisms is well known, and the present invention is applicable to any of these well-known effluent coagulation treatment methods and is not particularly limited.
本発明の好ましい実施態様では、活性汚泥と処理水とを
分離すべき分離領域におけるｐＨを７〜９、特に好まし
くは８．０〜８．５の範囲とすることによって一層優れ
た力値が得られる。In a preferred embodiment of the present invention, a more excellent strength value can be obtained by setting the pH in the separation area where the activated sludge and the treated water are to be separated to 7 to 9, particularly preferably 8.0 to 8.5. To be
本発明において凝集剤生産菌の添加量を培養液として
０．５重量％以上、好ましくは０．５〜１０重量％とす
る。凝集剤生産菌の濃度が１０％を越えて添加しても添
加量に応じて力値が向上するものでもなかった。In the present invention, the amount of the flocculant-producing bacterium added is 0.5% by weight or more, preferably 0.5 to 10% by weight, as a culture solution. Even if the concentration of the flocculant-producing bacterium was added in excess of 10%, the potency did not improve depending on the amount added.
（実施例） 従来公知のロードコッカス・エリスロポレスと、本発明
での凝集剤生産菌の夫々の培養液を下記の液体培地で３
０℃で振とう培養した。(Example) Each of the culture liquids of the conventionally known Rhodococcus erythropoles and the flocculant-producing bacterium of the present invention was mixed in the following liquid medium 3
The cells were shaken at 0 ° C.
尚、Ｒ−１菌は従来公知のロードコッカス・エリスロポ
レスであり、本発明のＲ−３菌はＫＹＭ−１株（FERM P
-11333）、ＫＹＭ−２株（FERM P-11334）、ＫＹＭ−３
株（FERM P-11335）、ＫＹＭ−４株（FERM P-11336）、
ＫＹＭ−５株（FERM P-11337）、ＫＹＭ−６株（FERM P
-11357）及びＫＹＭ−７株（FERM P-11358）からなって
いる。液体培地の組成 炭素源 １０ｇ／ K2HPO4 ５ｇ／ KH2PO4 ２ｇ／ MgSO4 ０．２ｇ／ (NH4)2SO4 ０．５ｇ／ イースト抽出物 ０．５ｇ／ NaCl ０．１ｇ／ （１）力価 （ａ）供試液 前記液体培地の炭素源としてフラクトースを使用し、ｐ
Ｈを８．５に調整した培地で５日間振とう培養した培養
液を供試液とした。温度は30℃とした。The R-1 bacterium is a conventionally known Rhodococcus erythropoles, and the R-3 bacterium of the present invention is a KYM-1 strain (FERM P
-11333), KYM-2 strain (FERM P-11334), KYM-3
Strain (FERM P-11335), KYM-4 strain (FERM P-11336),
KYM-5 strain (FERM P-11337), KYM-6 strain (FERM P-11337)
-11357) and KYM-7 strain (FERM P-11358). Composition of liquid medium Carbon source 10 g / K 2 HPO 4 5 g / KH 2 PO 4 2 g / MgSO 4 0.2 g / (NH 4 ) 2 SO 4 0.5 g / Yeast extract 0.5 g / NaCl 0.1 g / ( 1) Titer (a) Test solution Using fructose as a carbon source of the liquid medium, p
A culture solution obtained by shaking culture for 5 days in a medium in which H was adjusted to 8.5 was used as a test solution. The temperature was 30 ° C.
（ｂ）力値の評価法 １００ｍｌのメスシリンダーに、カオリン５０００ｍｇ
／液を８０ｍｌ入れ、無機カオリンと培養液を所定量
添加し、緩やかに転倒撹拌した後、０．５ＮのＮａＯＨ
及びＨＣｌでｐＨ７．０にｐＨを調整した。蒸留水で全
量を１００ｍｌとし、再度転倒撹拌後、静置し５分後の
上澄水のＯＤ550を測定し、１／Ｄ550にて凝集力価を表
示した。(B) Evaluation method of force value 5000 mg of kaolin was added to a 100 ml measuring cylinder.
/ 80 ml of liquid, add a predetermined amount of inorganic kaolin and culture liquid, gently invert and stir, then 0.5N NaOH
And pH was adjusted to pH 7.0 with HCl. The total volume was adjusted to 100 ml with distilled water, and after stirring again by inversion, the mixture was allowed to stand still and 5 minutes later, the OD 550 of the supernatant water was measured, and the agglutination titer was represented by 1 / D 550 .
Ｒ−１菌及びＲ−３菌の凝集力価の経日変化は第１図示
の通りであった。The daily changes in the agglutination titers of R-1 and R-3 bacteria were as shown in the first figure.
以上の通り本発明の菌Ｒ−３は他の菌に比較して著しく
優れた力価を示す。尚、Ｒ−１が従来公知のロードコッ
カス・エリスロポレス菌である。As described above, the bacterium R-3 of the present invention shows a remarkably excellent titer as compared with other bacteria. Incidentally, R-1 is a conventionally known Rhodococcus erythropoles bacterium.
（２）凝集時のｐＨ 凝集時のｐＨを５〜１０に調整し、最適凝集ｐＨについ
て検討した。(2) pH during aggregation The pH during aggregation was adjusted to 5 to 10 and the optimum aggregation pH was examined.
第２図示の様にｐＨが５から８に上昇するに従いＯＤ
550が低下し、それ以上のｐＨではＯＤ550が上昇し、培
養液の最適凝集ｐＨは８．０〜８．５であることが明ら
かとなった。As shown in Figure 2, OD increases as the pH increases from 5 to 8.
It was revealed that 550 decreased and OD 550 increased at pH higher than that, and the optimum aggregation pH of the culture solution was 8.0 to 8.5.
（効 果） 以上説明の様に、本発明の凝集剤生産菌は今回新たに凝
集剤生産能があることが発見されたものであり、従来公
知の凝集剤生産菌に比べて特にＲ−３菌と称されるシュ
ウドモナス属、アシネトバクター属、アグロバクテリウ
ム属、エンテロバクター属、オーレオバクテリウム属及
びオエルスコビア属からなる群から選ばれるの少なくと
も１属を含む微生物群は著しく優れた凝集剤生産能を有
する。(Effect) As described above, the flocculant-producing bacterium of the present invention has been newly discovered to have the ability to produce a flocculant, and R-3 is particularly superior to the conventionally known flocculant-producing bacterium. The microbial group including at least one genus selected from the group consisting of Pseudomonas, Acinetobacter, Agrobacterium, Enterobacter, Aureobacterium, and Oerscovia is called a bacterium and has a remarkably excellent flocculant-producing ability. Have.
従って、本発明方法によれば、優れた排水処理効果が得
られる。Therefore, according to the method of the present invention, an excellent wastewater treatment effect can be obtained.
第１図は凝集力価と経過日数との関係を示す図であり、
第２図は凝集力価とｐＨとの関係を示す図である。FIG. 1 is a diagram showing the relationship between agglutination titer and the number of days elapsed,
FIG. 2 is a diagram showing the relationship between aggregation titer and pH.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.5 識別記号 庁内整理番号 ＦＩ 技術表示箇所 (Ｃ１２Ｎ 1/20 Ｃ１２Ｒ 1:01） 審査官 佐伯 裕子 (56)参考文献 特公 昭52−31434（ＪＰ，Ｂ１) 植村定次郎等著「発酵と微生物ＩＩＩ」 （株）朝倉書店 （昭45．10．10） Ｐ．135ー166─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. 5 Identification number Internal reference number FI technical indication (C12N 1/20 C12R 1:01) Examiner Yuko Saeki (56) References Japanese Patent Publication No. 52-31434 (JP, B1) "Fermentation and Microorganisms III" by Sadajiro Uemura et al. Asakura Shoten Co., Ltd. (Sho 45.10.10) P. 135-166
Claims (1)
Ｍ−３株（FERM P-11335）、エンテロバクター属エンテ
ロバクターＫＹＭ−５株（FERM P-11337）、オーレオバ
クテリウム属オーレオバクテリウムＫＹＭ−６株（FERM
P-11357）及びオエルスコビア属オエルスコビアＫＹＭ
−７株（FERM P-11358）からなる群から選ばれる少なく
とも１種の凝集剤産生微生物。1. Acinetobacter KY, a genus of Acinetobacter
M-3 strain (FERM P-11335), Enterobacter genus Enterobacter KYM-5 strain (FERM P-11337), Aureobacterium genus Aureobacterium KYM-6 strain (FERM
P-11357) and Oelscovia genus Oelscovia KYM
-7 strain (FERM P-11358), at least one flocculant-producing microorganism selected from the group consisting of:
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2083078A JPH0661B2 (en) | 1990-03-30 | 1990-03-30 | Flocculant-producing microorganism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2083078A JPH0661B2 (en) | 1990-03-30 | 1990-03-30 | Flocculant-producing microorganism |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP4270755A Division JPH07108216B2 (en) | 1992-09-16 | 1992-09-16 | Microorganism-produced flocculant and wastewater flocculation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH03285676A JPH03285676A (en) | 1991-12-16 |
| JPH0661B2 true JPH0661B2 (en) | 1994-01-05 |
Family
ID=13792145
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2083078A Expired - Lifetime JPH0661B2 (en) | 1990-03-30 | 1990-03-30 | Flocculant-producing microorganism |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0661B2 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4573187B1 (en) * | 2009-07-01 | 2010-11-04 | 株式会社日本バイオマス研究所 | Sludge reduction method |
| CN102206025B (en) * | 2011-04-15 | 2013-01-02 | 苏州工业园区凯翔国际水技术有限公司 | Method for fully recycling sludge |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS582850B2 (en) * | 1975-09-01 | 1983-01-19 | 株式会社ボッシュオートモーティブ システム | Shyariyouyoureibo Sochi |
-
1990
- 1990-03-30 JP JP2083078A patent/JPH0661B2/en not_active Expired - Lifetime
Non-Patent Citations (1)
| Title |
|---|
| 植村定次郎等著「発酵と微生物ＩＩＩ」（株）朝倉書店（昭４５．１０．１０）Ｐ．１３５ー１６６ |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH03285676A (en) | 1991-12-16 |
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