JPH0662929B2 - Received light amount display material - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial application] The present invention relates to a novel light receiving amount display material, more specifically, a light blocking ratio of light of a specific wavelength is reduced according to the amount of light received. In other words, it is a simple and highly accurate received light amount display material in which the transmittance of the wavelength is increased. For example, in the case where the received light amount display material is red phycoerythrin extracted from algae cells, when phycoerythrin receives 550 nm The present invention relates to a simple light-receiving amount display material capable of measuring the light-receiving amount in the case of fading display, because the shielding of the light of the wavelength is reduced and the red phycoerythrin receives the light before receiving the light.

[Conventional technology]

There are various kinds of silver halides which have been conventionally used as a light-sensitive material in photography and the like, and diazonium compounds which are used in copy paper and the like.

However, these light-sensitive materials are compounds that develop color when they receive light, and they are sensitive to the initial light received. There are drawbacks such as difficulty in doing so.
Moreover, some of them have drawbacks such as high toxicity and high price.

[Problems to be Solved by the Invention]

The received light amount display material of the present invention is reduced in color by receiving light,
Moreover, it is a light-receiving material that fades according to the amount of received light and eventually becomes colorless and transparent, and the shielding rate decreases from the initial light reception to the latter period according to the received light amount, that is, the coloring substance is sensitive to the received light amount. Since the color fades to, the degree of color fading can be easily identified with the naked eye from the early stage to the latter stage of light reception, and there is no toxicity at all, and the material for indicating the amount of received light is simple and easy to handle.

As described above, the received light amount display material of the present invention is a novel received light amount display material in which the above drawbacks are improved.

[Means for solving problems]

The present invention has been made to solve the above-mentioned problems, and provides a material for indicating the amount of received light, which is characterized in that the color is reduced in accordance with the amount of the received light. More specifically, it is a simple light receiving amount display material in which the shielding rate of light of a specific wavelength is reduced in accordance with the amount of received light, in other words, the transmittance of the wavelength is increased. In the case of red-colored phycoerythrin extracted from algal cells, when phycoerythrin receives light, the light shielding rate of the wavelength of 550 nm decreases, and the red color is pink and colorless according to the light received by the red phycoerythrin before light reception. The present invention provides a simple and accurate received light amount display material in which the amount of received light can be easily measured with the naked eye depending on the degree of fading display.

In the present invention, the "ray" is natural light, artificial light, etc.,
Further, it may be a mixed light of these rays. The artificial light is, for example, light emitted from various fluorescent lamps, mercury lamps, halogen lamps, sunlight lamps, incandescent lamps, arc lamps, etc.,
The natural light may be any light such as sunlight, light having a specific wavelength absorbed from sunlight, and light having a certain ratio of the transmission of sunlight blocked.

The light ray is at least 280 to 700 nm, preferably 4
The amount of received light in the wavelength range of 50 to 600 nm, particularly preferably 480 to 590 nm, is accurately displayed or marked.

Further, "color reduction" is a decrease in the light shielding rate at a specific wavelength of the color that the received light amount display material has, and means fading of the specific color in view of the degree of color.

Further, the "light receiving amount display material" in the present invention may be any material as long as it is a material that reduces color corresponding to the received light amount, but is preferably a pigment extracted from algal cells, particularly preferably phycoerythrins, fucocyanins, Chlorophyll,
More preferably, fucoerythrins can be applied as the material. The phycocyanins include allophycocyanin B, allophycocyanin, C-fucocyanin, R-fucocyanin, fucoerythrocyanin, and the like,
Among them, C-phycocyanin is preferable, and the phycoerythrins include C-phycoerythrin, B-phycoerythrin, R-phycoerythrin, and the like, and among them, B-phycoerythrin is preferable.

The "algae" referred to in the present invention is mostly aquatic, and its cells are extremely simple such as simple substance, colony or system, and are plants that grow by photosynthesis. "Basics of plant phylogenetic classification" edited by Wang (May 1, 1975)
The plant described in 5th (published by Hokuryukan Co., Ltd.), and more specifically, lichens, cyanobacteria, red algae, yellow algae, orange algae, brown algae, They are Phytophthora phylum, Chlorophyta phylum and Rhizophyta phylum, preferably Red algae phylum, Cyanophyta phylum, and Green algae phylum, particularly preferably Red algae phylum.

In particular, phycoerythrin (phy
In the case of coerythrin), the red phycoerythrin aqueous solution has an absorption wavelength at 550 nm, and it receives 550 nm when receiving light rays.
The shielding rate of the light of the wavelength is decreased, and the red color fades to pink, transparent and colorless according to the light received by the red phycoerythrin before the light reception.

In addition, phycocyanin obtained from a cyanobacterial plant exhibits an eye color and fades as it receives light.

Also, chlorophyll obtained from a green algae plant exhibits a green color and fades as it receives light.

The light receiving amount display material of the present invention is used as it is by dissolving it in water or a solvent and putting the solution in a transparent test tube, or by impregnating the solution into a paper or cloth and using it as a litmus test paper. . Also, as a method of quantifying the amount of received light from the color-reduced display, it is easy to calculate the amount of received light by making a relationship diagram showing the amount of received light and the light blocking ratio or a standard fading sample and comparing it with the measured display. Therefore, the method for measuring the amount of received light is highly accurate.

〔The invention's effect〕

Thus, the material for displaying the amount of received light of the present invention enables simple and accurate measurement of the amount of received light. Simple solar radiation meter 2. Sunburn alarm (to enjoy moderate sunbathing) 3. 3. Sunlight measuring device for crops (to give the amount of sunshine commensurate with crops and to determine the harvest time of fruits, etc.) 4. Sunlight shortage alarm for pets 5. Photochemical reaction controller It can be used as a light quantity sensor of a light quantity measuring machine such as a light quantity measuring machine, and can be used in combination with a standard color difference meter to make a light receiving quantity measuring machine.

Hereinafter, the present invention will be described in more detail with reference to Examples and Reference Examples.

Example Porphylidium quertum (Porphyli) which is a red alga
dium cruentum) in the culture medium shown in Table 1 at a temperature of 28
While maintaining the temperature at 0 ° C., air containing 3% of carbon dioxide gas was constantly bubbled and cultured for 7 days. The grown algal cells were centrifuged, then suspended in the potassium phosphate buffer solution shown in Table-2, the algal membrane was disrupted by an ultrasonic cell disruptor, and the disrupted algal membrane was centrifuged. separated. Ammonia sulfate was added to the obtained supernatant so that the amount of ammonium sulfate was 25%, and the crude B-phycoerythrin salted out and precipitated was centrifuged. The obtained crude B-fucoerythrin was dissolved in the phosphate buffer solution shown in Table 2 to give crude B-
A 0.5% aqueous solution of fucoerythrin was obtained. Obtained crude B
The absorption characteristics of the phycoerythrin solution are shown in FIG.

This absorbance characteristic is reported in the literature (Chemistry and Biochemistry o
f Plant Pigments.Edited by TW, Goodwin.Volumel 3
28p-350p), which was in agreement with the absorbance characteristics of the B-phycoerythrin solution.

Reference Example A, Preparation of Phycoerythrin Aqueous Solution The B-phycoerythrin aqueous solution obtained in the example was diluted with pure water to prepare a 5 ppm aqueous solution of B-phycoerythrin (hereinafter referred to as solution A).

B, application example as a solar radiation meter 80% of the liquid A is filled in the glass container shown in FIG. 2, and it is hung on the apple tree shown in FIG. 3, and the received light amount of the liquid A and the liquid A The degree of fading was investigated. A black vinyl tape was wrapped around the light receiving portion of the glass container, and the outside was covered with aluminum foil so that the water temperature in the inside became 50 ° C. or less. The degree of fading was measured by measuring the light transmittance at 550 nm with a spectrophotometer. The results are shown in FIG. From FIG. 4, it can be seen that the shielding rate of the B-phycoerythrin solution decreased corresponding to the amount of received light of the solution A, and the red color of B-phycoerythrin faded. From Fig. 5, this fading is
It can be seen that the color is not discolored by the temperature rise due to the light reception, but is discolored only by the light reception amount.

[Brief description of drawings]

FIG. 1 is a wavelength-dependent characteristic diagram of the specific absorbance of the B-phycoerythrin solution of the example, and FIG. 2 is an integrated measuring instrument for the amount of solar radiation containing the B-phycoerythrin solution, a = 15.5 cm.
b = 3.0 cm c = 2.0 cm, FIG. 3 is a diagram of a usage example in which an integrated measuring device for solar radiation is hung on a tree, and FIG. 4 is a light transmittance and a light receiving amount (cal / cal at 550 nm). cm ² ), and FIG. 5 is a relationship diagram between the light transmittance at 550 nm and the temperature under dark conditions.

 ─────────────────────────────────────────────────── ─── Continuation of the front page (56) References JP-A-55-149812 (JP, A) “Chemical Dictionary 7” edited by the Editorial Committee for Chemistry Dictionary (37-8-5) Kyoritsu Shuppan P. 642-643 “Chemical Dictionary 3” edited by the Editorial Committee for Chemistry (Sho 35-9-30) Kyoritsu Shuppan P. 275 ~ 276

Claims (9)

[Claims] 1. A received light amount display material having a reducing effect by receiving light, which contains a pigment extracted from algal cells as an active ingredient. 2. The received light amount display material according to claim 1, wherein the color reduction is a reduction of a light shielding rate at 550 nm. 3. The received light amount display material according to claim 1, wherein the subtractive color is fading of red light. 4. The received light amount display material according to claim 1, wherein the light rays are natural light and / or artificial light. 5. The light receiving amount display material according to claim 1, wherein the light rays are light rays in a wavelength range of at least 280 to 700 nm. 6. The light receiving amount display material according to claim 1, wherein the light rays are light rays in a wavelength range of at least 450 to 600 nm. 7. The first aspect of the present invention is that the algae are red algae.
The received light amount display material described in the item. 8. The received light amount display material according to claim 1, wherein the received light amount display material is at least one selected from phycoerythrin, phycocyanin, chlorophyll and the like. 9. The received light amount indicating material according to claim 8, wherein the phycoerythrin is B-phycoerythrin.