JPH0669359B2 - Fluidized bed type anaerobic bioreactor- - Google Patents
Fluidized bed type anaerobic bioreactor-Info
- Publication number
- JPH0669359B2 JPH0669359B2 JP29285686A JP29285686A JPH0669359B2 JP H0669359 B2 JPH0669359 B2 JP H0669359B2 JP 29285686 A JP29285686 A JP 29285686A JP 29285686 A JP29285686 A JP 29285686A JP H0669359 B2 JPH0669359 B2 JP H0669359B2
- Authority
- JP
- Japan
- Prior art keywords
- fermenter
- carrier
- pump
- rotating plate
- fluidized bed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/04—Bioreactors or fermenters specially adapted for specific uses for producing gas, e.g. biogas
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/16—Particles; Beads; Granular material; Encapsulation
- C12M25/20—Fluidized bed
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/18—Flow directing inserts
- C12M27/20—Baffles; Ribs; Ribbons; Auger vanes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/18—Flow directing inserts
- C12M27/24—Draft tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- General Chemical & Material Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Centrifugal Separators (AREA)
Abstract
Description
【発明の詳細な説明】 産業上の利用分野 この発明は、二相式メタン発酵法における後段のメタン
発酵過程に用いる流動床型嫌気性バイオリアクターに関
するものであって、特に発酵槽内に担体を投入してかん
菌を付着させ、発酵の効率化を計ったものである。Description: TECHNICAL FIELD The present invention relates to a fluidized bed type anaerobic bioreactor used in a subsequent methane fermentation process in a two-phase methane fermentation method, and more particularly to a carrier in a fermenter. It was added to allow the bacilli to adhere and to improve the efficiency of fermentation.
従来の技術 二相式メタン発酵法は酸発酵過程とメタン発酵過程の2
段階の操作を行って有機物の分解能力の向上を計るもの
であり、後段のメタン発酵過程では低分子化された有機
酸の分解能力の確保が要件である。Conventional technology The two-phase methane fermentation method consists of an acid fermentation process and a methane fermentation process.
It is intended to improve the decomposition ability of organic substances by performing a step operation, and in the latter stage of methane fermentation process, it is a requirement to secure the decomposition ability of low-molecular organic acids.
このメタン発酵過程に用いられるバイオリアクターとし
ては従来、懸濁方式の装置、例えば、連続式完全混合型
リアクターが用いられている。As a bioreactor used in this methane fermentation process, a suspension type device, for example, a continuous complete mixing type reactor has been conventionally used.
発明が解決しようとする問題点 前記したようにメタン発酵過程では低分子化された有機
酸の分解能力の確保が主要点であるが、処理能力を上げ
るためには菌体の生棲密度を高める必要がある。しかし
メタン菌体の増殖速度は非常に遅く、菌体の世代交代時
間は1.5〜8日といわれ、前記従来の懸濁方式の連続発
酵では希釈率が大きいために菌体のウオッシュアウトが
生じ発酵槽の処理能力を高めることができなかった。こ
のため発酵槽内に菌体を付着させる流動床方式が考えら
れているが、発酵槽内に菌体を直接付着させることは困
難であり、好結果が得られない。Problems to be Solved by the Invention As described above, in the methane fermentation process, the main point is to secure the ability to decompose low-molecular-weight organic acids, but in order to increase the processing capacity, increase the viable cell density. There is a need. However, the growth rate of methane cells is very slow, and it is said that the generation alternation time of cells is 1.5 to 8 days. In the conventional continuous fermentation of the suspension system, the washout of the cells occurs due to a large dilution rate, which causes fermentation. The processing capacity of the tank could not be increased. For this reason, a fluidized bed system in which the bacterial cells are attached to the fermenter has been considered, but it is difficult to directly attach the bacterial cells to the fermenter, and good results cannot be obtained.
問題点を解決するための手段 この発明の流動床方式は、発酵槽内に菌体の付着し易
い、すなわち比重1.5〜3.5程度の多孔質の担体を投入
し、菌体を高濃度で保持しようとするものであって、こ
の場合、担体に付着しやすいメタン菌体はかん菌である
から、この発明においては比較的世代交代時間の長いか
ん菌を担体に付着させることにより、発酵槽内の菌体の
濃度を高めかつ菌体の滞留時間が大きく、しかも発酵液
体の滞留時間が短くなるようにするため、担体を内部液
の非流動時は槽底に沈降静止させ、流動時は槽内に分散
させて発酵の効率化を計ったものである。Means for Solving the Problems In the fluidized bed system of the present invention, cells are easily attached to the fermenter, that is, a porous carrier having a specific gravity of about 1.5 to 3.5 is added to keep the cells at a high concentration. In this case, since the methane bacterium that easily attaches to the carrier is bacillus, in the present invention, by attaching bacilli having a relatively long generation alternation time to the carrier, In order to increase the microbial cell concentration, increase the microbial cell retention time, and shorten the fermentation liquid retention time, the carrier is allowed to settle at the bottom of the tank when the internal liquid does not flow, and inside the tank when flowing. It is a product of which the efficiency of fermentation is measured by dispersing it in the.
発明の実施例 この発明の実施例に図面について説明すると、第1図は
この発明バイオリアクターの1例の全体構造を示す正面
断面図、第2図は、このバイオリアクターの回転板部分
の作用を示す断面説明図、第3図はこの回転板の外周面
に付設された凹条の構造を示す斜視図であって、第1図
において、1は発酵槽であって、上端部分を外側方に張
り出す段付円筒状の密閉容器として作られ、かつ内部上
方には上方よりみて輪状の受とい2が支架取付されてい
る。この受とい2の底部は管3ならびにポンプ4を介し
て発酵槽1底部と連通しており、また受とい2の下端に
は垂下縁5が連設され、その内面適所に一端を開口して
外部に通ずる排出管6が開閉弁7と共に設けられてい
る。そして発酵槽1の下端に近い側面には原料供給管8
がポンプ9と共に設けられている。Embodiments of the Invention Referring to the drawings for the embodiments of the present invention, FIG. 1 is a front sectional view showing the overall structure of an example of the bioreactor of the present invention, and FIG. 2 shows the action of the rotating plate portion of the bioreactor. 3 is a perspective view showing the structure of a groove provided on the outer peripheral surface of the rotary plate, and in FIG. 1, 1 is a fermenter, and the upper end portion is outward. It is formed as a stepped cylindrical hermetic container, and a ring-shaped support 2 is attached to the upper part of the inside by a bridge. The bottom of the tray 2 communicates with the bottom of the fermenter 1 via the pipe 3 and the pump 4, and the lower edge of the tray 2 is provided with a hanging edge 5 and one end is opened at a proper position on the inner surface thereof. A discharge pipe 6 communicating with the outside is provided together with an on-off valve 7. The raw material supply pipe 8 is provided on the side surface near the lower end of the fermenter 1.
Are provided together with the pump 9.
次に、10は回転板であって、発酵槽1上端を貫通して垂
設された立軸の下端に取りつけられ、槽体上面に設けた
モーター11により高速回転されるようになっている。12
はモーター11回路に接続されたインバータである。この
回転板10は、皿状部10aを囲んで上面が外方に傾く傾斜
面10bに形成され、周面下端に円周を等分して数個の翼
板10c…を突設するとともに、下面中央部を空洞10dと
し、かつ空洞10dと傾斜面10bとを連ねる通路10eを設け
た一体のブロックとして形成され、上記周縁を受とい2
より、若干上方に現わし、翼板10c…を受とい2内に位
置させて設けられている。そして外面には傾斜面10bか
ら周面の翼板10c…にわたって各複数個の切欠部10g…を
設けた多数の凹条10f…が放射状に付設され、かつ傾斜
面10bの周縁にはリブ10hが形成されている。Next, 10 is a rotating plate, which is attached to the lower end of a vertical shaft penetrating the upper end of the fermentation tank 1 and rotated at a high speed by a motor 11 provided on the upper surface of the tank body. 12
Is an inverter connected to the motor 11 circuit. The rotating plate 10 is formed on an inclined surface 10b that surrounds the dish-shaped portion 10a and has an upper surface inclined outward, and a plurality of blades 10c ... The central portion of the lower surface is formed as a cavity 10d, and is formed as an integral block provided with a passage 10e that connects the cavity 10d and the inclined surface 10b.
Thus, the vanes 10c ... On the outer surface, a large number of recessed lines 10f are provided radially from the inclined surface 10b to the blades 10c on the peripheral surface, each of which is provided with a plurality of notches 10g, and ribs 10h are provided on the periphery of the inclined surface 10b. Has been formed.
作用、効果 この発明は上記のように構成され、発酵槽1内の予め菌
体を付着した担体13を収容し通常時は、ポンプ9を介し
て原料供給管8から原料液を供給するとともに、開閉弁
7を用いて内溶液を外部に取り出す。Action, effect The present invention is configured as described above, accommodates the carrier 13 to which the bacterial cells have been attached in advance in the fermenter 1, and normally supplies the raw material liquid from the raw material supply pipe 8 via the pump 9, The on-off valve 7 is used to take out the internal solution to the outside.
いま、排出管6の開閉弁7を閉じ、次いでポンプ4を駆
動すると、第2図の矢印で示されるように、発酵槽1下
部から内部液が吹き上げられ、槽下部に沈澱していた担
体13をまき上げて上昇し、回転板10下面の空洞10dから
通路10eを通って回転板10の上面に出て皿状部10aに溜
り、溢流して傾斜面10b上面に分散する。次いでモータ
ー11を駆動して回転板10を高速で回転すると、液中の担
体は遠心力により吹きとばされて発酵槽1の内壁1に衝
突し、自重により落下して槽底に戻るが、液中の水は凹
条10f…に流入して切欠部10gを通って、回転板10の周面
から側方にとび受とい2の周壁内面2に受けとめられ、
受とい2内に貯溜され、ポンプ4を介して槽下部に循環
される。なお、前記担体の流下に当っては発酵槽1壁に
オリフィス1aを設けて回収した担体が戻るところを負圧
になるようにすると担体の戻りを容易に行うことができ
る。Now, when the on-off valve 7 of the discharge pipe 6 is closed, and then the pump 4 is driven, the internal liquid is blown up from the lower part of the fermentation tank 1 as shown by the arrow in FIG. And rises, goes out from the cavity 10d on the lower surface of the rotating plate 10 through the passage 10e to the upper surface of the rotating plate 10, accumulates in the dish-shaped portion 10a, overflows, and is dispersed on the upper surface of the inclined surface 10b. Next, when the motor 11 is driven to rotate the rotating plate 10 at a high speed, the carrier in the liquid is blown off by the centrifugal force and collides with the inner wall 1 of the fermentation tank 1 and falls by its own weight to return to the bottom of the tank. The water in the liquid flows into the ridges 10f, passes through the notches 10g, is laterally skipped from the peripheral surface of the rotary plate 10, and is received by the inner surface 2 of the peripheral wall of the catcher 2.
It is stored in the receiving tray 2 and circulated to the lower portion of the tank via the pump 4. In addition, when the carrier flows down, an orifice 1a is provided on the wall of the fermenter 1 so that the recovered carrier has a negative pressure so that the carrier can be easily returned.
さて、上記回転板10は高速回転して、担体と水とを分離
するが、この回転板10の周面下端には翼板10c…が付設
されているから、分離効率が上昇しすぎて、すなわち受
とい2内に水が必要以上に溜る場合は、この翼板10c…
が抵抗となって回転板10の回転を低下させ、こうして常
に適切な分離が行われるようになっている。Now, the rotary plate 10 rotates at a high speed to separate the carrier and the water, but since the blades 10c ... Are attached to the lower end of the peripheral surface of the rotary plate 10, the separation efficiency increases too much, In other words, if more water than is necessary is stored in the saucer 2, this blade 10c ...
Acts as a resistance to reduce the rotation of the rotary plate 10, and thus proper separation is always performed.
しかも、上記構成により、担体の循環は遠心力により槽
体内壁に沿わせ、次いで自重により槽壁に沿って流下
し、ポンプによる強制循環を受けないから、担体の破砕
や付着菌体の分散は防止され極めて効率的な処理を行う
ことができる。Moreover, with the above configuration, the carrier is circulated along the inner wall of the tank by centrifugal force, and then flows down along the tank wall by its own weight, and is not subjected to forced circulation by the pump, so that the carrier is not crushed or the adherent cells are dispersed. It can be prevented and extremely efficient processing can be performed.
第1図は、この発明のバイオリアクターの1例の正面断
面図、第2図は回転板の作用を説明するための断面図、
第3図は回転板の外周面に付設された凹条の構造を示す
斜視図である。 1…発酵槽、2…受とい、3…管、4,9…ポンプ、6…
排出管、7…開閉弁、8…原料供給管、10…回転板、11
…モーター、10c…翼板、10f…凹条、13…担体FIG. 1 is a front sectional view of an example of a bioreactor of the present invention, and FIG. 2 is a sectional view for explaining the action of a rotating plate,
FIG. 3 is a perspective view showing the structure of a groove provided on the outer peripheral surface of the rotary plate. 1 ... Fermenter, 2 ... Receptacle, 3 ... Pipe, 4,9 ... Pump, 6 ...
Discharge pipe, 7 ... Open / close valve, 8 ... Raw material supply pipe, 10 ... Rotating plate, 11
… Motor, 10c… Vane, 10f… Recess, 13… Carrier
Claims (1)
平面輪状の受とい(2)を支架取付するとともに、上記
受とい(2)の若干上方に上下面連通し、かつ周面の上
端から下端にわたって、各複数個の切欠部を設けた凹条
(10f)を多数付設した回転板(10)を、その周面下端
に付設した翼板(10c)を受とい(2)内に位置させて
垂下軸設し、さらにこの発酵槽には上記受とい内部と発
酵槽底部とをポンプ(4)を介して連ねる管(3)、一
端を受とい下方に開口し、開閉弁(7)を介して他端が
外部に通ずる排出管(6)、下端に近くポンプ(9)を
介して一端が槽内に開口する原料供給管(8)を各配設
してなる流動床型嫌気性バイオリアクター。1. A fermenter (1) in the form of a closed container, above the inside of
A flat ring-shaped bridge (2) is mounted on a supporting frame, communicates with the upper and lower surfaces slightly above the bridge (2), and a plurality of cutouts are provided from the upper end to the lower end of the peripheral surface ( A rotating plate (10) having a large number of 10f) attached thereto, a blade plate (10c) attached to the lower end of the peripheral surface of the rotating plate (10), which is positioned in the receiving shaft (2), is provided with a hanging shaft. A pipe (3) that connects the inside and the bottom of the fermenter via a pump (4), a discharge pipe (6) that opens at the lower end to receive one end, and the other end communicates to the outside through an open / close valve (7), the lower end A fluidized bed type anaerobic bioreactor in which raw material supply pipes (8) each having one end opening into the tank via a pump (9) are provided.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP29285686A JPH0669359B2 (en) | 1986-12-09 | 1986-12-09 | Fluidized bed type anaerobic bioreactor- |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP29285686A JPH0669359B2 (en) | 1986-12-09 | 1986-12-09 | Fluidized bed type anaerobic bioreactor- |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS63146780A JPS63146780A (en) | 1988-06-18 |
| JPH0669359B2 true JPH0669359B2 (en) | 1994-09-07 |
Family
ID=17787254
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP29285686A Expired - Fee Related JPH0669359B2 (en) | 1986-12-09 | 1986-12-09 | Fluidized bed type anaerobic bioreactor- |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0669359B2 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2808036B2 (en) * | 1990-05-25 | 1998-10-08 | 神鋼パンテツク株式会社 | Three-phase stirred fluidized bed bioreactor |
| US7875448B2 (en) | 2004-01-12 | 2011-01-25 | Single Use Brx, Llc | Bioreactor systems and disposable bioreactor |
-
1986
- 1986-12-09 JP JP29285686A patent/JPH0669359B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPS63146780A (en) | 1988-06-18 |
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