JPH0676289B2 - Disease prevention agent for agriculture and horticulture - Google Patents

Description

TECHNICAL FIELD The present invention relates to an agricultural and horticultural disease control agent, and more particularly to a control agent effective against soft rot and gray mold of agricultural products.

[Prior art and its problems] Soft rot and Botrytis cinerea are important diseases of agricultural products. Currently, for the control of these diseases, soft rot is streptomycin agent (antibiotic), copper agent (inorganic copper, organic copper), etc., and gray mold disease is procymidone agent, thiophanate methyl agent, iprodione agent, copper agent (inorganic copper). , Etc. have been used, but recently, resistance bacteria against other chemicals except copper chemicals have been recognized, and the efficiency of chemical control tends to decrease. Further, copper agents are restricted in terms of use because they are likely to cause chemical damage.

In order to avoid the occurrence of this resistant bacterium, chemical damage and the like, it is desired to develop a novel disease onset preventive agent which is different from conventional control agents.

On the other hand, social concern about environmental pollution of pesticides or pesticide residue problems is high, and from this aspect, pesticides derived from natural products with low toxicity and high biodegradability are strongly demanded.

[Means for Solving the Problems] As a result of intensive studies on the development of a control agent satisfying the above requirements, the present inventors have found that a mixture of manganese, boron and rutin exhibits a control effect against soft rot and gray mold. The present invention has been achieved by obtaining new knowledge.

That is, the present invention is an agricultural and horticultural disease preventive agent containing manganese, boron, and rutin.

The composition of the present invention is conventionally used as a "plant growth promoting composition" (Japanese Patent Publication No. 49-31789) as a composition for promoting plant growth, increasing yield, and improving quality. It was not known at all that this composition had a controlling effect against pathogenic bacteria.

Hereinafter, the configuration of the present invention will be described in detail.

Of the components contained in the composition of the present invention, manganese and boron are used as trace elements of plants for the purpose of improving growth. To further describe these ingredients, boron is a preservative for wood and leather [Nobuhito Ohta "Synthesis, insecticide, germicide, herbicide" page 289 (Showa 27) Gihodo],
In addition, at a concentration of 4000 mg /, Penicillium chrysogen
It is said to suppress the growth of um bacteria [Bowen: Plant pysi
ole 41, pages 319 to 324 (1960)].

Rutin has a diuretic effect, an antihistamine effect on animals,
There are various actions such as anti-hyaluronidase action and antibacterial action on fungi, but this has not been confirmed due to controversy [Crampton: Science, 110, 18 (1948)] Thus, the composition of the present invention Is used as a composition for promoting the growth of plants, and boron is said to exhibit weak bactericidal properties when used alone. However, the present inventors have mixed three chemical substances of manganese, boron, and rutin, Erwinia caroto, a soft rot fungus that is an important pathogen of agricultural and horticultural crops
For the first time, we have found that there is a remarkable synergistic effect against Bora fungus, and we have obtained completely new knowledge that it has a controlling effect against Botrytis cinerea, which is an important disease of crops.

The composition according to the present invention can be used for disease control of crops, rutin is one component contained in plants such as buckwheat, and is also used as a medicine, manganese, and compounds each containing boron are inorganic normally. Therefore, the agricultural product using this composition is a safe mixed composition which is safe as a food and does not cause environmental pollution.

From this point of view, the composition of the present invention is a very significant composition which has both plant production promoting effect and disease controlling effect in food production.

[Examples] Next, the present invention will be described with reference to Examples, but the present invention is not limited to the following examples. In the following examples, parts are based on weight unless otherwise specified.

Formulation example (per 100 parts of the composition of the present invention) Manganese sulfate monohydrate 0.5 to 3.75 parts Borax decahydrate 1.5 to 8.25 parts Rutin 1.8 to 13.5 parts Distilled water balance Example 1 Soft rot of various crops with plant pathogens Erwinia caroto causes disease
bora and non-pathogenic Bacillus cereus IAM 1729 (blasticidin S assay), and Pseudomonas fluore
cens NIHJ B254 (Kasugamycin test bacterium) was used as a test bacterium.

Use Czapek agar as the medium and add 20 ml to a Petri dish with a diameter of 9 cm.
Was added to Czapek agar by adding an appropriate amount of the culture bacterium of the bacterium prepared in advance to make a suspension, and 4 ml of the suspension was dispensed on the base layer to form a seed layer. On this, a paper disc (diameter 8 mm) for antibiotics test was impregnated with the present composition shown in the following formulation, and the excess liquid was removed with filter paper (paper disc method) and placed in PDA medium at 28 ° C. After being left to stand for 48 hours, the bacterial growth inhibition width was measured to investigate the antibacterial activity.

Prescription: Manganese sulfate monohydrate 2.5 parts Borax decahydrate 5.5 parts Rutin 9.0 parts Distilled water 83.0 parts The results are shown in Table 1.

As a result, it was found that the composition according to the present invention has a particularly high bactericidal activity against the soft rot fungus Erwinia carotovora having pathogenicity.

Example 2 E. carotovora having the highest bactericidal activity according to Example 1
According to the method of Example 1 using bacteria, manganese sulfate monohydrate 2.5 parts, distilled water 97.5 parts (Mn liquid), borax decahydrate 5.5
Parts, distilled water 94.5 parts (B liquid), rutin 9 parts, distilled water 91 parts (R liquid), each single liquid, manganese sulfate monohydrate 2.5 parts, rutin 9 parts, distilled water 88.5 parts (Mn-R) Liquid), Borax decahydrate
5.5 parts, rutin 9 parts, distilled water 85.5 parts (BR solution), manganese sulfate monohydrate 2.5 parts, borax decahydrate 5.5 parts, distilled water
By using 92 parts (Mn-B liquid) of each two-agent mixed liquid and the composition liquid (Mn-B-R liquid) of the formulation example of the above-mentioned Example 1 in the same manner as in Example 1, the antibacterial effect was obtained by the paper disk method. The activity was assayed.
The results are shown in Tables 2 and 3.

It can be seen from the table that the mixture of manganese, boron and rutin, which is the composition of the present invention, has a wider inhibition width than the single liquid and the two-component mixture, the inhibition circle is clear, the bactericidal activity is remarkably high, and a synergistic effect is exhibited. I understand.

Example 3 5 radishes (no radish when cultivated for early fatting) were sown in a pot with a diameter of 12 cm, and 10 days after germination, the cotyledon was sprayed with the test solution at 50 ml / m ² and dried, and then soft rot fungus (E.carotovora) Bacterial suspension obtained by liquid culture was inoculated with a needle by wounding, left standing in a humid chamber at 30 ° C for 24 hours, the diameter of lesion spots formed on cotyledons was measured, and the control effect was investigated. The results are shown in Table 4, and the composition of the present invention containing 25 of the mixture of manganese, boron and rutin was used.
The 0-fold diluted solution showed a control value of about 56% for controlling soft rot.

Example 4 Gray mold fungus (Botr) which is an important disease in plant cultivation of vegetables
ytis cinerea) sprayed with a predetermined concentration diluted aqueous solution of the composition shown in the formulation example per 50 ml / m ² of cucumber seedling cotyledons (variety Tokiwa ground cucumber), dried solution, and then pre-cultured in PDA medium A fungal disease-section of a mold of 5 mm in diameter was inoculated and allowed to stand at 20 ° C under humid conditions for 48 hours, and the diameter of the resulting lesion was measured and the control effect was investigated. The results are shown in Table 5, and a 500-fold diluted solution of the composition of the present invention, a mixture of manganese, boron and rutin had a controlling effect of about 60% when used for controlling gray mold.

Example 5 The present agent was applied to a diseased part of Mycophaerella melonis, which first appeared on melon (cultivation Earlsfeborgit) stalks grown in greenhouse, and 15 days later, lesions were measured to measure the spread prevention effect. . As a control foliar, a 1/1000 dilution of TPN wettable powder is 200/1
Sprayed per 0a. The results, as shown in Table 6, showed a remarkable controlling effect by applying the undiluted solution of the composition of the present invention, which is a mixture of manganese, boron and rutin, to melon stems infected with vine wilt.

[Effect of the Invention] As described above, the composition containing manganese, boron and rutin according to the present invention exerts an effective controlling effect against soft rot, gray mold and vine blight of agricultural products.

 ─────────────────────────────────────────────────── ─── Continuation of front page (56) References JP-A-59-231006 (JP, A) JP-B-49-31789 (JP, B2)

Claims (1)

[Claims] 1. An agricultural and horticultural disease preventive agent comprising manganese, boron and rutin.