JPH0683624B2 - Small animal detection composition, small animal detection member and small animal detection method - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION <Industrial field of application> The present invention relates to a composition, a member and a method useful for detecting the presence / absence of small animals, and more specifically, to easily confirm the presence / absence with the naked eye. It is intended to provide a composition for detecting small animals, a member for detecting small animals, and a method for detecting small animals that can reliably and easily detect the presence or absence of small animals that are difficult or require a long time for confirmation.

<Prior art and its problems> Small animals called so-called hygienic pests such as mites and lice are not only a vector for infectious diseases but also blood-sucking, biting, parasitism, etc. Give pain.

On the other hand, in recent years, housing has become more hermetically sealed due to technological advances,
Moreover, because carpets are often laid in the room, for example, it is the current situation that a convenient environment for mites to live is created, which promotes the reproduction of mites. The fact is that extermination is required because it becomes a source of allergy such as.

However, since small animals such as mites and lice are not only small, but generally have small gaps as their homes, it is difficult to easily confirm their existence with the naked eye, and observe them carefully for a long time to see their movements. It is complicated to check with a loupe or the like.

In addition, the control of agricultural pests such as spider mites other than the above-mentioned sanitary pests, thrips, and aphids is a complicated means such as observing with a magnifying glass or the like, the pests adhering to the leaves or stems are transferred directly or to an adhesive sheet or the like. It is carried out by confirming the existence or reproduction of habitat and spraying pesticides, but the reality is that a simpler detection method is desired.

<Means for Solving Problems> The present invention provides a novel composition for detecting small animals, a member for detecting small animals and a method for detecting small animals, which solves the problems of the prior art. However, firstly, a substance having an adhesive property and a compound that develops a color by reacting with a small animal and a body fluid are contained as essential components, and secondly, a substance having an adhesive property on a substrate reacts with the body fluid of a small animal. A small animal detection adhesive layer comprising a compound that develops color is provided, and thirdly, the captured small animal is crushed to react and develop color with the compound that develops color by reacting with the body fluid of the small animal. There are things to do.

According to the present invention, mites, hygienic pests such as lice, spider mites, thrips, aphids, small pests such as agricultural pests such as nematodes (nematodes) can be easily caught, and after capture small animals The presence or absence of a small animal can be easily detected with the naked eye by crushing to produce a body fluid and reacting this body fluid with a specific chromogenic compound to develop a color.

The composition for detecting small animals of the present invention contains, as an essential component, a substance having an adhesive property and a compound that develops a color by reacting with a body fluid of a small animal.

Examples of the substance having adhesiveness include a film made of the substance,
Formed by laminating, coating, spraying, etc. on the surface of a base material such as a sheet or a molded article by an appropriate means such as extrusion, roll, brush, spray, etc., and arranging it in a place where small animals are supposed to live, or these As long as the small animal can be adhered and captured on the material layer surface by means such as adhering to the surface of the place, for example, the carpet surface, it may be liquid or semi-solid at room temperature, for example, oils, softeners, Examples thereof include liquid rubber, liquid resin, natural proteins or polysaccharides, polymeric substances, pressure-sensitive adhesive substances containing rubber and / or synthetic resin as a main component, and adhesive hydrous gel.

The compound that develops color by reacting with body fluids of small animals is not particularly limited as long as it is a compound having such performance, and examples thereof include ninhydrin, sodium 2,4,6-trinitrobenzenesulfonate, proca dyeing solution (for example, ZIEHL carboxylic acid. Fuchsin red liquor: LoFFLER methylene blue liquor: distilled water = 8: 1
0:10 (ml)) and the like.

It is desirable that such a compound is contained in the composition in an amount of 0.01 to 20% by weight, preferably 0.05 to 5% by weight, but the compound may be added in an amount exceeding this range.

It is also possible to use enzymes contained in the body fluids of small animals. For example, as the compound, 4-aminoantipyrine, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid, 0-phenyl) is used. Nilendiamine, etc.,
A substrate consisting of hydrogen peroxide can also be used, and the substrate develops a color by enzymatic reaction with peroxidase in the body fluid of small animals.

In the above, glucose is generated by the presence of water instead of hydrogen peroxide, and a reagent such as glucose oxidase is used to cause color reaction by reacting water in the body fluid with peroxidase. It is a good one.

Any of the above substrates can be included in the composition in the range of 0.5-20 mM.

The composition for detecting small animals is a substance having an adhesive property as described above and a compound that develops a color by reacting with a body fluid of a small animal as an essential component, but if necessary, an attractant, a colorant,
An antiaging agent or the like can be added.

The use of an attractant is useful when the film, sheet or molded article formed with the composition is placed in a place where small animals are likely to live, and the attractant can be used to attract and capture the composition. As the attractant, an aggregation pheromone,
Kylomon, food attractant, etc. can be mentioned, and generally 0.01 to 50% by weight can be added to the whole composition.

Further, the colorant is used in the composition so that the color developed by the reaction between the compound and the body fluid of a small animal is clearly visible, and is usually a pale color tone such as white or yellow. , Such as zinc white, calcium carbonate, white titanium, white lead, zinc sulfide, etc., or those that are not reactive with the color formers such as dyes and pigments in the range of 0.1 to 50% by weight in the total composition. Can be compounded.

The composition for detecting small animals thus constituted is generally 5 to 2000 μm on a substrate such as various plastic films or sheets, glass, paper, cloth and the like, or films, sheets or molded articles made of laminates thereof. It is preferable to laminate, coat or spray with the above-mentioned forming means in advance with a thickness of, for example, the composition is poured into a spray container or another container, and a suitable film, sheet or molded product is used at the time of use. It may be sprayed or applied on the surface of the base material.

In the above, when the composition is previously formed on the surface of a base material such as a film, the substance having the adhesive property and the compound are formed in a mixed system on the surface of the base material such as the film to form a small animal detection adhesive layer. For example, a substance layer having an adhesive property is formed on the surface of a base material by the means as described above, and the compound alone or a compound having a diluting or spreading function such as the compound and the solvent is formed on the surface of the layer. It is also possible to form a mixture layer with a substance to form a multi-layer system small animal detection adhesive layer.

In the latter case, the thickness of the compound alone or the mixture layer is 10 μm
The following is preferable, and if it exceeds this, the trapping property of the adhesive layer may be hindered, which is not preferable. In the above, the positions of the substance layer and the compound alone or the mixture layer may be reversed.

In a member provided with such a small animal detection pressure-sensitive adhesive layer, it is useful to color the substrate itself when the pressure-sensitive adhesive layer is substantially transparent. The coloring is selected such that the color developed by reacting with the compound and the body fluid of the small animal is easily visible, and the coloring is compounded with a colorant in the base material itself, or a colorant is applied to at least one surface of the base material. This can be done by coating or pasting.

The coloring of the base material or the adhesive layer is also useful when a specific color (yellow) such as thrips exerts an attracting effect. For example, a yellow colored sheet is releasably attached to the back surface of a transparent base material. In addition, it can be peeled off upon detection.

As described above, the composition for detecting small animals or the member for detecting small animals constituted as described above is arranged at a place where small animals are supposed to live, or wound in a roll with the adhesive layer surface facing outside. Rotate and roll it on the surface of a place where small animals are supposed to live to catch small animals.

The member on which the small animal has been captured in this way is folded in two and the adhesive layer surfaces are overlapped with each other, or a transparent or colored cover film (for protecting the adhesive surface is attached to the adhesive layer surface. The peelable film used or the like can also be used.) The work of crushing the small animals by applying pressure is performed by bonding the peelable film or by rolling the peelable roll. At this time, if a small animal is captured on the sticky surface of the member, the body fluid reacts with the compound or the like to develop a color, and the presence or absence of the small animal at that location can be easily confirmed visually. Moreover, by crushing the small animal by pressure, the body fluid of the small animal is diffused,
The area of color development is larger than that of the small animal body, and detection is reliable and easy.

In the detection method of the present invention, a small animal is captured by a member provided with a substance layer having an attractant as an attractant on the surface of the base material, and a compound that develops a color by reacting with the body fluid of the small animal at the time of detection is sprayed by a spray or the like After that, it is detected by the above method, or a substance layer having an adhesive property on the surface of the substrate, and a mixture of the attractant and the compound is sprayed and then provided for capturing a small animal. To be done.

<Effect> According to the present invention, if a small animal is inhabited, it can be reliably captured, and after capture, the small animal is crushed to react with the body fluid and the compound to develop color in a short time. In addition, it has a feature that it can be easily detected.

Moreover, since the body fluid is spread by crushing, the color development area is larger than the size of the small animal body, so it is easy to see, and the compound reacts only with the small animal body fluid, and it should be taken for a long time with human dandruff and dirt. If you do not react because it does not react, and the color that develops due to the reaction between the body fluid of the small animal and the compound can be assumed in advance,
There is also an advantage that detection is easy.

<Examples> Examples of the present invention will be shown below, but the present invention is not limited to the following examples.

Example 1 35 g of an acrylic pressure-sensitive adhesive solution (solid content 35% by weight) obtained by copolymerizing 95 g of butyl acrylate and 5 g of acrylic acid in toluene was mixed with 1 g of ninhydrin, 10 ml of acetone and 5 ml of n-butanol. Then, it was dissolved uniformly to obtain a composition for detecting small animals.

Next, a polyethylene terephthalate film (thickness: 25 μm) whose surface is plasma-treated with the composition has a thickness after drying.
It was applied so as to have a thickness of 100 μm and dried at 50 ° C. for 3 hours to obtain a small animal detection member.

The sticking surface was pressed against this member against the back surface of the indoor carpet, which is believed to be home to the aphids and mites that lived on the back surface of the pear leaf, for capture work.

After the capture work, the aphids could be visually identified if they were watched, but it was unknown whether or not the ticks had dandruff or dirt on them.

Next, when a transparent plastic film was attached to the adhesive surface of this member and a pressing operation was performed with a rubber roll, and the state of color development was observed, color development as shown in Table 1 was observed and could be easily detected.

Example 2 95 g of butyl acrylate, 2 g of methacrylic acid and 3 of acrylic acid
Acrylic pressure-sensitive adhesive solution (solid content 10 wt%)
Then, sodium 2,4,6-trinitrobenzenesulfonate (TNBS) was dissolved at a concentration of 5% to obtain a composition for detecting small animals.

Next, the composition was applied to a polyethylene terephthalate film (thickness 50 μm) so that the thickness after drying would be 100 μm, and dried at 50 ° C. for 3 hours to obtain a small animal detection member.

An adhesive surface was pressed against this member on the front and back surfaces of a carpet in a room where mites are likely to live, and a capturing operation was performed.

After the capture work, fold the member in half and overlap the adhesive surfaces,
When pressure was applied with a rubber roll and the color development was observed, spotted spots of ocher color with a diameter of about 2 mm were observed, which was easily detected.

For comparison, when the same detection work was carried out with the one without TNBS, no color was observed and it was not possible to detect it because it was indistinguishable from dandruff and dirt.

Example 3 95 g of butyl acrylate and 5 g of acrylic acid were copolymerized in toluene to obtain an acrylic thick adhesive solution (solid content: 45
50% by weight), 50 ml of 10% ninhydrin acetone solution,
20 ml of toluene was mixed and uniformly dissolved to obtain a composition for detecting small animals.

Next, the composition was applied to a transparent polyethylene terephthalate film having a thickness of 50 μm so that the thickness after drying would be 100 μm, and dried at 50 ° C. for 30 minutes to obtain a small animal detection member.

A yellow plastic film was releasably attached to the back surface of this member, and the film was suspended for 3 days at room temperature where thrips were inhabited for capturing.

After the capturing work, the yellow plastic film was peeled off, the white plastic film was attached to the adhesive surface, and the colored state was observed by applying pressure with a rubber roll,
Spotted spots of purple color with a diameter of about 3 mm were easily detected.

Note that the captured slips are about 1 m on the adhesive surface before color development.
Since it was close to transparent around m, it was difficult to distinguish dust and the like.

Example 4 The composition for detecting small animals shown in Table 2 was sprayed or brushed onto a plastic sheet having a thickness of 300 μm to give a composition of about 5-50.
It was applied so as to have a thickness of μm, and this was set at 24 o'clock in a room where mites are likely to live, and capture work was performed.

After the capturing operation, a plastic sheet was attached to the adhesive layer surface, and a pressure operation was performed with a rubber roll to observe the color development state. As a result, color formation with a diameter of about 2 to 3 mm was observed, which was easily detected.

Example 5 Polysaccharide pullulan produced by Aureobasidium pullulans, which is one of molds, is heated in distilled water to dissolve it in approximately the same amount as water, and TNBS is dissolved in 2.5% of this and cooled to room temperature. Thus, a composition for detecting small animals was obtained.

Next, the composition was applied to the surface of a roll having a diameter of 5 cm and dried until the surface became sticky to obtain a small animal detection member.

This member was rolled along the back surface of the leaf, which is believed to have infested with mites, and the capturing work was performed.

After the capturing operation, the member was peeled off and rolled on the plastic film while applying pressure to observe the color-developed state.Orange coloring was observed on the surface of the roll-shaped member. I was able to detect it.

Example 6 On the surface of a polyester non-woven fabric having a thickness of 150 μm, 2% by weight of gelatin, 5 mM of 4-aminoantipyrine and 5 mM of hydrogen peroxide (A), 2% by weight of gelatin and 2,2′-azino-bis (3-ethyl) were used. Benzothiazoline-6-sulfonic acid 5 mM and hydrogen peroxide 5 mM (B), and 2% by weight of gelatin and 0-
Solutions of 5 mM phenylenediamine and 5 mM hydrogen peroxide (C) were applied to obtain small animal detection members.

This member was brought into close contact with the back surface of the leaf, which is believed to be infested with spider mites, and a capturing operation was performed.

After the capturing work, a plastic film was attached to the surface of the member, and the pressure was applied with a rubber roll to observe the color development state. (A) was red, (B) was turquoise, (C)
Each of the oranges had its own color, which was easily detected.

Example 7 When 16% by weight of polyvinyl alcohol was used in place of 2% by weight of gelatin in Example 6, the detection operation was performed in the same manner as in Example 6, and the same coloring state as in Example 6 was obtained. showed that.

Claims (7)

[Claims] 1. A composition for detecting small animals, which comprises, as essential components, a substance having an adhesive property and a compound that develops a color by reacting with a body fluid of a small animal. 2. The composition for detecting small animals according to claim 1, wherein the content of the compound that develops a color when reacted with the body fluid of a small animal is 0.01 to 20% by weight. 3. A compound which develops a color upon reaction with a body fluid of a small animal is at least one selected from the group consisting of ninhydrin, sodium 2,4,6-trinitrobenzenesulfonate and proca dyeing solution. The composition for detecting small animals according to item 2. 4. A member for detecting small animals, which comprises an adhesive layer for detecting small animals, which comprises a substance having an adhesive property and a compound which develops a color by reacting with a body fluid of a small animal, on a substrate. 5. The member for detecting small animals according to claim 4, wherein the adhesive layer for detecting small animals is a mixed system of a substance having an adhesive property and a compound which develops a color by reacting with a body fluid of a small animal. 6. The member for detecting small animals according to claim 4, wherein the small animal detecting adhesive layer is a multilayer system of a substance layer having an adhesive property and a compound that develops a color by reacting with a body fluid of the small animal. 7. A method for detecting a small animal, which comprises detecting a presence of a small animal by crushing the captured small animal and causing a compound that develops a color by reacting with the body fluid of the small animal and a body fluid of the small animal to react and develop color.