JPH0688028B2 - Fast-acting urolith dissolving agent - Google Patents

Description

TECHNICAL FIELD The present invention relates to a microorganism-based fast-acting urolith solubilizer.
In other words, it is based on microorganisms and has high cleanliness.
In particular, the present invention relates to a fast-acting urinary stone dissolving agent that rapidly decomposes and removes the urinary stone components adhering to the toilet bowl and its surroundings by the action of microorganisms to suppress the excrement odor, and also has a fragrance to impart aroma.

[Prior Art] As a conventional urinary stone removal method, there is an attempt to (1) dissolve or exfoliate by using a chemical containing an acid or an alkali as a main component. (2) There is an attempt to scrape off by mechanically applying an external force. (3) There is an attempt to decompose or remove it by using a preparation containing a mixture of microorganisms and enzymes. Or (4)
The above (1) and (2) are used in combination.

In such a conventional method, (1) a method using a chemical containing an acid or an alkali as a main agent has a high risk of eroding a toilet bowl, a tile or the like and invading the skin or clothes of a cleaner.
(2) A large amount of labor is required to mechanically scrape off an external force. (3) With conventional preparations containing a mixture of microorganisms and enzymes, decomposition takes time, and urinary stones cannot be promptly removed.

[Problems to be Solved by the Invention] Accordingly, the present invention has conducted diligent research so as to solve the conventional problems, and as a result, together with the microorganisms, glucose and nitrogen sources have been used as carbon sources necessary for the growth of the microorganisms. As a result, by adding ammonium chloride, it was possible to promote the growth of microorganisms, enhance the urine stone decomposing ability, and significantly shorten the treatment time. Therefore, it is an object of the present invention to provide a microorganism-based urinary stone decomposing agent capable of rapidly decomposing and removing urine stones and the like sticking to a toilet bowl and the like and almost eliminating odor. Another object of the present invention is to form a urinary stone degrading agent by combining the above-mentioned microbial component with glucose, ammonium chloride, and a perfume component, and to provide a microorganism-based urinary stone degrading agent body having the perfume effect.

[Means for Solving Problems] The gist of the present invention is that glucose is added to 20 parts by weight per 100 parts by weight of a microbial component composed of a microorganism, a medium and a carrier.
It is a fast-acting urolith-dissolving agent characterized by containing -50 parts by weight, 1-5 parts by weight of ammonium chloride, and 3-5 parts by weight of a perfume ingredient. And, preferably, the microbial component is composed of 8 or more kinds of microorganisms therein, and 1 g of the microbial component
The number of bacteria against Bacillus is 10 ⁸ to 10
¹² / g, Streptococcus genus 10 ⁷
~ 10 ¹⁰ / g, Aspergillus genus 10 ⁴ ~
10 ¹² / g, Cellulomonas spp 10 ⁷ ~ 10 ⁹
/ g, Rhizopus genus fungus 10 ⁴ ~ 10 ¹² / g, Nitrosomonas (Nitrosomonas) genus fungus 10 ⁶ ~ 10 ⁹ / g, Nitrobacter (Nitrobacter) genus fungus 10 ⁷ ~ 10 ⁹ / g, The bacterium belonging to the genus Pseudomonas is 10 ⁶ to 10 ¹⁰ / g.

In order to provide a microbial-based urinary stone degrading agent having an excellent urolith degrading effect, the present inventors have used a microbial component as a microorganism,
For 100 parts by weight of the microbial component composed of the medium and the carrier, for example, the number of bacteria per 1 g of the microbial component is Bacillus genus 10 ⁸ -10 ¹² / g, Streptococcus genus 10 ⁷ 〜 10 ¹⁰ / g, fungus of the genus Aspergillus 10 ⁴ ~ 10 ¹² / g, Cellulomonas (Ce
llulomonas) 10 ⁷ ~ 10 ⁹ / g, Rhizopu
s) fungus 10 ⁴ -10 ¹² / g, Nitrosomona
s) Fungus 10 ⁶ -10 ⁹ / g, Nitrobacter
A combination of 10 ⁷ to 10 ⁹ / g of bacteria of the genus and 10 ⁶ to 10 ¹⁰ / g of bacteria of the genus of Pseudomonas is added, and the odor that cannot be completely treated by the microbial components alone is supplemented with a fragrance to complete the treatment. It is intended to provide a urinary stone degrading agent having a function of preventing foul odor generation and degrading waste.

Examples of the microorganism used in the microorganism-based urolith-degrading agent of the present invention include Bacillus genus cells, Streptococcus genus cells, and Aspergillus (Asp).
ergillus genus, Cellulomonas genus, Rhizopus genus, Nitrosomonas genus, Nitrobacte
r) The bacterium belonging to the genus Pseudomonas is contained.

The microbial component of the microorganism-based urinary stone degrading agent of the present invention is composed of a microorganism, a medium and a carrier, and as the medium, a certain soybean meal that is a food for the microorganism used and has a certain composition is clear. Agricultural product by-products such as rice bran, bran, and bagasse are used, or rotten beverages such as milk are used, and yeast extract, malt extract, and other chemicals can be used in combination as the case may be.

Further, the carrier used in the present invention serves as a residence for the microorganism used, and preferably, in the case of an inorganic powder (including a porous body), calcium carbonate, magnesium carbonate,
Using calcium silicate powder, in the case of organic powder,
Scraps and the like can be used.

Regarding the urinary stone degrading agent blending ratio of the present invention, the number of bacteria present in the microbial component composed of the microorganism, the medium and the carrier is a bacterium of the genus Bacillus, 10 ⁸ to 10 ¹² / g, Streptococcus. (Streptococcus) genus, 10 ⁷ ~ 10
¹⁰ / g, a bacterium of the genus Aspergillus, 10 ⁴ ~
10 ¹² / g, cellulomonas (Cellulomonas), 10 ⁷
~ 10 ⁹ / g, Rhizopus bacteria, 10 ⁴ ~ 10 ¹²
/ g, Nitrosomonas genus, 10 ⁶ ~ 1
0 ⁹ / g, a bacterium of the genus Nitrobacter, 10 ⁷ ~
10 ⁹ / g, Pseudomonas bacteria, 10 ⁶
Contains ~ 10 ¹⁰ / g.

To 100 parts by weight of this microbial component, further 1 microbial component
Glucose 20 to 50 parts by weight is added to 00 parts by weight to complete the urolith decomposition effect. Glucose less than 20 parts by weight does not effectively promote the growth of microorganisms and is inconvenient, and when it exceeds 50 parts by weight, the number of bacteria of the genus Bacillus and Pseudomonas increases excessively, and with bacteria of other species. Coexistence becomes impossible.

The urinary stone degrading agent of the present invention further contains 1 to 5 parts by weight of ammonium chloride and 3 to 5 parts by weight of a perfume component with respect to 100 parts by weight of the microbial component.

If the amount of ammonium chloride is less than 1 part by weight, the growth of microorganisms is not effectively promoted, which is inconvenient. If the amount of ammonium chloride exceeds 5 parts by weight, the urine stone decomposing ability is deteriorated and ammonia odor is caused.

Further, if the amount of the fragrance component is less than 3 parts by weight, the odor of the microbial component cannot be masked, which is inconvenient. If the amount of the fragrance component exceeds 5 parts by weight, the odor of the fragrance becomes too strong.

As the fragrance component, any toilet fragrance made by Cloister Chemicals can be used, such as D-0715 and D-
0829 is preferred.

In addition, the microorganisms in the microbial component are composed of 8 or more species, and the number of bacteria per 1 g of the microbial component is 10 ⁸ to 10 ¹² / g in Bacillus genus, and Streptococcus (Streptococcus).
us) 10 ⁷ ~ 10 ¹⁰ / g, Aspergill (Aspergill)
us) 10 ⁴ 〜 10 ¹² / g, cellulomona (Cellulomona)
s) bacteria of 10 ⁷ to 10 ⁹ / g, Rhizopus bacteria of 10 ⁴ to 10 ¹² / g, Nitrosomonas bacteria of 10 ⁶ to 10 ⁹ / g, Nitrobacter ( Nitrobacter) contains 10 ⁷ to 10 ⁹ / g and Pseudomonas contains 10 ⁶ to 10 ¹⁰ / g.

That is, the bacterium of the genus Bacillus, if it is less than 10 ⁸ / g, the ability to decompose urinary stone components is insufficient, and if it exceeds 10 ¹² / g,
It causes the limit of growth in the coexistence with other species of bacteria and also causes malodor.

In addition, if the bacteria of the genus Streptococcus (Streptococcus) is less than 10 ⁷ / g, the ability to decompose urinary stone components is insufficient, and if it exceeds 10 ¹⁰ / g, decomposition processing ability is not required anymore, It becomes the limit of growth in the coexistence with the bacteria of the species.

When the bacterium of the genus Aspergillus is less than 10 ⁴ / g, the ability to decompose urinary stone components is insufficient, and when it exceeds 10 ¹² / g, the growth limit in coexistence with other species of bacteria is limited.

When the cellulomonas bacterium is less than 10 ⁷ / g, the ability to decompose urinary stones is insufficient, and when it exceeds 10 ⁹ / g,
Limiting growth in coexistence with other species of bacteria.

The added Rhizopus fungus secretes a very powerful saccharifying enzyme, so that carbohydrates in urinary stones can be rapidly converted into sugars, and the effect of Bacillus can be greatly enhanced. The saccharides and hydrocarbons thus obtained are decomposed by the bacterium of the genus Streptococcus. Therefore, when the bacterium of the genus Rhizopus is mixed, the decomposition of filth and malodorous source can be completed in an extremely short time. If the bacterium of the genus Rhizopus is less than 10 ⁴ / g, the ability to decompose urinary stones is insufficient, and if it exceeds 10 ¹² / g, the growth limit in coexistence with other species of bacteria is limited.

Nitrosomonas (Nitrosomonas) genus bacteria is less than 10 ⁶ / g, ammonia NO ₂ ^- insufficient to alter the, 10
If it exceeds ⁹ / g, it will limit the growth in coexistence with other species of bacteria.

In addition, the bacteria of the genus Nitrobacter are 10 ⁷ / g
Below is not sufficient to convert NO ₂ ⁻ to NO ₃ ⁻ , 10
^When it exceeds ⁹ , the limit of growth in coexistence with other species of bacteria is reached.

If the Pseudomonas bacterium is less than 10 ⁶ / g, it is insufficient to change NO ₃ to N ₂ , and if it exceeds 10 ¹⁰ / g, it grows in coexistence with other species of bacteria. Will be the limit.

In the present invention, these eight kinds of bacteria are mixed to promptly decompose and remove urinary stones. That is, Bacillus
us) Streptococcus
Rhiz, Aspergillus spp, Cellulomonas spp, Rhiz spp.
The bacterium of the genus opus and the bacterium of the genus Nitrosomonas contributes to the decomposition of urinary stone components such as uric acid and organic substances attached to the surface of the urinary stone, and facilitates the exfoliation of the urine stone.

Nitrosomonas bacteria, Nitrobacter bacteria, and Pseudomonas bacteria contribute to the decomposition of a large amount of ammonia generated from urinary stones. , Has the action of removing the bad smell of ammonia. In other words, Nitrosomonas (Nitrosomonas) genus of bacteria, ammonia → NO ₂ ^-
To, Nitrobacter (Nitrobacter) genus bacteria, NO ₂ ^- → N
O ₃ ^- to and Pseudomonas (Pseudomonas) genus bacteria, NO ₃ ^- → converted into N _2, it is intended to suppress malodors with ammonia.

Then, a bacterium belonging to the genus Cellulomonas decomposes the cellulose component in the urine stone to some extent and is useful for exfoliating the urine stone. And Cellulomona
If the amount of the genus s) is less than 10 ⁷ / g, it is insufficient for decomposing the cellulose, and if it exceeds 10 ⁹ / g, the growth limit in the coexistence with other species is limited.

For the above reason, the microorganism-based urinary stone degrading agent of the present invention has the above-mentioned mixing ratio.

As described above, in the urinary stone decomposition agent body of the present invention, these 8
It is intended to decompose urinary stone components in a well-balanced manner by mixing different types of bacteria. Then, the above-mentioned eight kinds of bacteria perform their respective functions to decompose the urinary stone component and facilitate peeling. Moreover, the malodor in the urinary stone decomposing agent can be masked off due to the fragrance component having the aroma that is simultaneously produced.

In addition, the genus Bacillus used for the microorganism-based urinary stone degrading agent of the present invention is particularly preferably Bacillus subtilis [IAM (Institute of Applied Microbio
logy; Abbreviation for useful strain preservation facility of the Institute for Applied Microbiology, University of Tokyo; hereinafter also referred to as this abbreviation) 1168] is suitable, but in addition to this, B. natto [IFO
(Abbreviated name of Institute for Fermentation Osaka: Fermentation Research Institute; hereinafter abbreviated to this abbreviation) 3009] bacteria,
Bacillus coagulans [IAM 111
5], B. macerans [IAM 124
3] may be used together.

Any bacterium of the genus Streptococcus may be used, but S. faecalis [IAM 1119], Streptococcus cremoris [IAM 1150] and lactis (S. faecalis).
lactis) [IAM 1198] and the like can be used.

Moreover, as a bacterium of the genus Aspergillus,
Aspergillus oryzae (IFO 4176),
Aspergillus niger (E.niger) [IFO 4066] and Aspergillus usamii [IFO 6082] can be used.

In addition, as one fungus of the genus Rhizopus of mold, Rhizopus formosaensis (Rhizopus for
mosaensis) [IAM 6250], Rhizopus oryzae (Rhi
zopus oryzae) [IAM 6006], Rhizopus pseudochinensis [IAM 6042] and the like can be used.

And as a bacterium of the genus Cellulomonas,
Cellulomonas biazotea (IFO 1268
0], C. cellasea [IFO 375
3], Cellulomonas Uda (IFO 3747), and the like.

And as a bacterium of the genus Nitrosomonas, Nitrosomonas (N. europaea) [IF
O 14298] and the like can be used.

As the bacterium of the genus Nitrobacter, Nitrobacter agilis [IFO 14297] and the like can be used.

Examples of Pseudomonas bacteria include P. nitroreducens.
[IFO 12694], Pseudomonas caryophili (Pc
aryophilli) [IFO 12950] and P. stutzeri [IFO 3773] can be used.

As the medium used for the microbial component of the present invention, agricultural product wastes such as soybean dregs and bran can be used, and yeast extract and malt extract can be used in combination depending on the case. Further, as the carrier used for the microbial component, powder of calcium carbonate, magnesium carbonate, calcium silicate or the like is used.

[Action] The mechanism of sewage treatment of the microorganism-based rapid-acting urolith-decomposing agent of the present invention is considered to be as follows.

That is, a bacterium of the genus Bacillus decomposes organic substances such as starch and proteins, which mainly emit a malodor in urinary stone components, and decomposes sugars and hydrocarbons produced by the decomposition into Streptococcus cells and Rhizopus. (Rhizopu
s) The bacterium belonging to the genus rapidly decomposes further, promotes fermentation, and removes malodorous substances. And Bacillus
s) genus cells, Streptococcus genus cells, Aspergillus genus cells and Rhizopus genus cells decompose urolith components.

In addition, the nitrosomonas (Nitros
The genus omonas, the genus Nitrobacter, and the genus Pseudomonas contribute to the decomposition of a large amount of ammonia generated in the urinary stone component. → NO ₂ ^- to,
Nitrobacter genus body, NO ₂ ^- to, Pseudomonas Shokukintai ^{_{is, NO 3 - - → NO 3}} → the N _2, is obtained can solve odor problems ammonia in these three genera cells . Then, the cellulosic component in the urinary stone component is partially decomposed by the cellulomonas cells.

The urinary stone degrading agent of the present invention can be produced as follows. That is, water is added to the above-mentioned microbial components (microorganism + medium + carrier) and mixed at 25 to 28 ° C for 24 to 48 hours, and the final water content is adjusted to 5 to 6 by using a vacuum dryer or a spray dryer. %
And dried to powder. Thereafter, the dried microbial component produced in this manner, glucose, ammonium chloride, a predetermined amount of a fragrance component, or, if desired,
Water is mixed to produce the urolith-degrading agent of the present invention in an appropriate particle size.

Next, the method of using the urinary stone degrading agent of the present invention, when used on the urinary stone component attached to the toilet bowl, has high activity of microorganisms, can prevent an offensive odor, and facilitates exfoliation of urine stones. Is.

In the urinary stone degrading agent, a large amount of microorganisms are present and activated,
Completely decomposes and ferment odors and excrement.

The microorganism-based urolith-decomposing agent of the present invention is particularly fast-acting and suitable for decomposing urolith components in a short time. Furthermore, it is also suitable for other wastewater and wastewater treatment.

Next, the microorganism-based urinary stone degrading agent of the present invention will be described with reference to specific examples, but the present invention is not limited to the following examples.

[Example] Twelve microorganisms were used, and 2 × 10 ⁹ / B. Subtilis [IAM 1168] bacteria of the genus Bacillus were used.
g. B. macerans [IAM 1243]
2 × 10 ⁹ / g of bacteria, Streptococcus
Genus Streptococcus faecalis
[IAM 1119] bacteria 4 × 10 ⁸ / g, Aspergillus oryzae (E.oryzae) [IFO 4176] 5 × 10 ⁵ / g, Aspergillus niger (E.niger) [IFO 4066] 5 × 10 ⁵ / g and 5 × 10 ⁵ / Rhizopus R.formosaensis [IAM 6250] bacteria of the genus Rhizopus
g, Cellulomonas of the genus Cellulomonas
2 × 10 ⁷ cells of C.biazotea [IFO 12680]
/ g, Nitrosomonas genus Nitrosomonas (N.europaea) [IFO 14298] 6 × 10 ⁷ / g, Nitrobacter genus Nitrobacter agirisu (N.agilis) [ IFO 14297] bacterial cells 8 × 10 ⁷ / g, Pseudomonas (Pseudomonas) genus Pseudomonas nitro les du sense (P.nitroreducens) [I
FO twelve thousand six hundred ninety-four] bacterial cells 4 × 10 ⁶ / g, Pseudomonas, Kyariofiri (P.caryophilli) [IFO 12950] The 4 × 10 ⁶ /
g, Pseudomonas stutzeri [IFO
3773] in an amount of 2 × 10 ⁶ / g in an amount of 60 parts by weight, 25 parts by weight of soybean debris, 5 parts by weight of yeast extract, 15 parts by weight of malt extract, 25 parts by weight of calcium carbonate and 1000 parts by weight of water. After weighing and mixing, and culturing while mixing at 25 ° C. for 24 hours, it was dried by a vacuum dryer and a spray dryer until the final water content became 6 to 4% by weight, and a powdered product was obtained. Bacillus bacteria are contained in this powder body at 6 × 10 ¹¹ / g,
Streptococcus spp. 4 × 10 ¹⁰ / g, Aspergillus spp 7 × 10 ⁸ / g and Rhizopus spp 4
× 10 ⁸ / g, 3 cells of the genus Cellulomonas
× 10 ⁸ / g was present.

Furthermore, 5 × 10 ⁸ / g of bacteria of the genus Nitrosomonas, 6 × 10 ⁸ / g of bacteria of the genus Nitrobacter, and 4 × 10 ⁸ of bacteria of the genus Pseudomonas. ^{There was 8} / g.

To 100 parts by weight of this microbial component, 3 parts by weight of D-0829 manufactured by Cloister Chemicals, 50 parts by weight of glucose, and 5 parts by weight of ammonium chloride were uniformly mixed as a flavoring component to give a fast-acting urinary stone. Used as a dissolving agent.

The following tests were carried out on the thus obtained microorganism-based urinary stone degrading agent.

That is, with respect to 100 parts by weight of water having a water temperature of about 30 ° C., an aqueous solution obtained by adding 0.05 parts by weight of the fast-acting urolith dissolving agent is used.
It was observed whether urine stones attached to the inside (under the eye plate), outside (helicopter), or the periphery of the men's toilet can be easily removed.

For the inside of the men's toilet (under the eye plate), seal the bottom part of the eye plate and inject the aqueous solution. For the outside (heli) and the surrounding area, soak the aqueous solution in absorbent paper and attach it. It was carried out by placing it on a urine stone. After a lapse of a fixed time, that is, 1, 2, 4, and 6 hours after the start of the test, the peelability of urinary stones was investigated using a driver or a scoop. At the same time, the pH and the amount of ammonia were measured.

As control tests, conventional microorganisms, enzyme preparations (Control A), the above-mentioned fast-acting urolith solubilizers without glucose and ammonium chloride (Control B), and water with a water temperature of about 30 ° C (blind test) The same test was performed using the same.

The above test conditions and test results are summarized in Table 1.
In addition, the amount of ammonia when untreated is 260 ppm or more, pH
It was 9.7.

From Table 1, the following is clear.

The peelability of urinary stones is evaluated in the following order: easy removal (including dissolution)> easy removal> difficult removal.

For urinary stone removal, Control A was 6 hours after the start of the test,
Control B showed an effect in 4 hours, whereas the fast-acting urolith dissolving agent of the present invention showed a clear effect in 1 hour, and at the same time suppressed the generation of ammonia and lowered the pH value. .

[Effects of the Invention] The microorganism-based urinary stone degrading agent of the present invention is a urinary stone treating agent that is liable to generate a bad odor, and by adding microorganisms of eight genera, the nutrients are added to enhance the biological activity of these microorganisms. Since glucose and ammonium chloride were added as a source, firstly, there was provided a urinary stone decomposing agent that promptly removes urine stones that are difficult to remove and cause a bad odor during toilet cleaning. Bacillus contained
Genus, Streptococcus genus, Aspergillus genus, Cellulomo
Bacteria of the genus nas) and the genus Rhizopus decompose uric acid, which is the main component of urinary stones, and organic substances attached to the surface of urinary stones. Since it proliferates actively as a nutrient source and can exert its ability in a short time, compared with the conventional microbial enzyme preparation, the treatment time required for clean and easy removal of urinary stones can be significantly increased. It has become possible to shorten it. Third, Nitrosomonas (Nitr
The odor of ammonia can be suppressed by the combination of bacteria of the genus (osomonas), bacterium of the genus Nitrobacter, and bacteria of the genus Pseudomonas, and the addition of glucose and ammonium chloride can be performed in a shorter time than before. Ammonia can be decomposed, and fourthly, remarkable technical effects such as providing a urinary stone decomposing agent that can be rapidly decomposed are obtained.

Claims (2)

[Claims] 1. To 100 parts by weight of a microbial component composed of a microorganism, a medium and a carrier, 20 to 50 parts by weight of glucose, 1 to 5 parts by weight of ammonium chloride, and 3 to 5 parts by weight of a perfume component are blended. Characteristic fast-acting urolith dissolving agent. 2. The microbial component according to claim 1, wherein the microbial component is composed of 8 or more species, and the number of bacteria per 1 g of the microbial component is 10 ⁸ to 10 ¹² / g of bacteria belonging to the genus Bacillus. , Streptococcus spp. 10 ⁷ to 10 ¹⁰ /
g, fungus of the genus Aspergillus 10 ⁴ to 10 ¹² /
g, Cellulomonas spp. 10 ⁷ to 10 ⁹ / g,
Rhizopus genus 10 ⁴ -10 ¹² / g, Nitrosomonas genus 10 ⁶ -10 ⁹ / g, Nitrobacter genus 10 ⁷ -10 ⁹ / g, Pseudomonas ) A fast-acting urinary stone solubilizer according to claim 1, wherein the bacterium belonging to the genus 10 ⁶ to 10 ¹⁰ / g.