JPH0695073B2 - Sample holder for fluorescence measurement - Google Patents
Sample holder for fluorescence measurementInfo
- Publication number
- JPH0695073B2 JPH0695073B2 JP63216270A JP21627088A JPH0695073B2 JP H0695073 B2 JPH0695073 B2 JP H0695073B2 JP 63216270 A JP63216270 A JP 63216270A JP 21627088 A JP21627088 A JP 21627088A JP H0695073 B2 JPH0695073 B2 JP H0695073B2
- Authority
- JP
- Japan
- Prior art keywords
- sample holder
- fluorescence
- fluorescence measurement
- sample
- film
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000005259 measurement Methods 0.000 title claims description 22
- 229910052751 metal Inorganic materials 0.000 claims description 27
- 239000002184 metal Substances 0.000 claims description 27
- 229910052782 aluminium Inorganic materials 0.000 claims description 18
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 18
- 230000005284 excitation Effects 0.000 claims description 17
- 239000004033 plastic Substances 0.000 claims description 15
- 239000011521 glass Substances 0.000 claims description 14
- 230000001678 irradiating effect Effects 0.000 claims description 2
- 230000000149 penetrating effect Effects 0.000 claims 1
- 229910001220 stainless steel Inorganic materials 0.000 claims 1
- 239000010935 stainless steel Substances 0.000 claims 1
- 239000010408 film Substances 0.000 description 24
- 230000000694 effects Effects 0.000 description 11
- 239000011888 foil Substances 0.000 description 8
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 6
- 238000007740 vapor deposition Methods 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 239000000539 dimer Substances 0.000 description 4
- 229920006130 high-performance polyamide Polymers 0.000 description 4
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 3
- 239000004793 Polystyrene Substances 0.000 description 3
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 3
- 229910052804 chromium Inorganic materials 0.000 description 3
- 239000011651 chromium Substances 0.000 description 3
- 229920002223 polystyrene Polymers 0.000 description 3
- 239000010409 thin film Substances 0.000 description 3
- 238000001771 vacuum deposition Methods 0.000 description 3
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 239000010453 quartz Substances 0.000 description 2
- 229910052709 silver Inorganic materials 0.000 description 2
- 239000004332 silver Substances 0.000 description 2
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 238000010876 biochemical test Methods 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000007772 electroless plating Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229920006332 epoxy adhesive Polymers 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 239000011253 protective coating Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000004544 sputter deposition Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N21/0303—Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
Landscapes
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Optical Measuring Cells (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Description
【発明の詳細な説明】 〔産業上の利用分野〕 本発明は、蛍光測定装置に使用するための蛍光測定用試
料保持体に関するものである。TECHNICAL FIELD The present invention relates to a sample holder for fluorescence measurement for use in a fluorescence measurement device.
励起光を試料上方より照射し、試料上方に発する蛍光成
分を集光してその蛍光強度を測定するためには、一般に
特開昭62−50662号公報の実施例に記載されているよう
な装置を使用する。In order to measure the fluorescence intensity by irradiating excitation light from above the sample and condensing the fluorescence component emitted above the sample, an apparatus generally described in the example of JP-A-62-50662 is used. To use.
ところで、周知のように蛍光を測定するための試料保持
体、例えば蛍光測定用のセルには、石英ガラスセル、ガ
ラスセル、ポリスチレンなどのプラスチック製のセルな
どがある。石英ガラスセルは、紫外光に対する透過性が
よく、セル自体の蛍光が他のガラスやプラスチック製の
セルに比べて少ないため、頻繁に使用されている。By the way, as is well known, a sample holder for measuring fluorescence, for example, a cell for measuring fluorescence includes a quartz glass cell, a glass cell, a plastic cell such as polystyrene, and the like. Quartz glass cells are often used because they have good transparency to ultraviolet light and the fluorescence of the cells themselves is less than that of other glass or plastic cells.
ガラスセルやプラスチック製のセルは紫外光、特に360n
m以下の光に対して蛍光を生じるため、通常可視域（360
〜700nm）の光で蛍光体を励起する場合に使用される。
さらに、これらの材料は石英ガラスに比べ安価であり、
また成型が比較的簡単であるため、ティスポーザブルセ
ルとして使用される。特に生化学検査などでの場合のよ
うに、測定する検体数が多数の場合は、通常、特開昭58
−213253などに記載されているように既知のプラスチッ
ク製マイクロプレートが使用される。Glass cells and plastic cells use UV light, especially 360n
Fluorescence is generated for light of m or less, so it is usually in the visible range (360
Used to excite the phosphor with light of ~ 700 nm).
Furthermore, these materials are cheaper than quartz glass,
Since it is relatively easy to mold, it is used as a disposable cell. Especially when a large number of specimens are to be measured, as in the case of biochemical tests, etc.
Known plastic microplates are used as described in −213253 and the like.
しかし、He−Cdレーザ光（325nm）、N2レーザ光（337n
m）やHgランプからの紫外領域の光など励起光の波長が
紫外領域になると、プラスチックではもちろん通常のガ
ラスでもそれ自体蛍光を発するようになる。そのため、
プラスチック製のマイクロプレートを使用して蛍光測定
を行うと、試料からの蛍光の他にマイクロプレートから
の蛍光が検出され、試料に含まれる微量の蛍光体からの
蛍光測定が困難になるという問題がある。However, He-Cd laser light (325 nm), N 2 laser light (337 n
m) and the wavelength of the excitation light such as the light from the Hg lamp in the ultraviolet region, the ordinary glass, as well as plastic, will fluoresce. for that reason,
When fluorescence measurement is performed using a plastic microplate, fluorescence from the microplate is detected in addition to fluorescence from the sample, which makes it difficult to measure fluorescence from a minute amount of fluorophore contained in the sample. is there.
紫外領域の光で励起する場合、試料を保持する試料保持
体の材質は一般に石英ガラスでなければならない。しか
し、石英ガラスは非常に高価であり、ディスポーザブル
にはできず、多数の試料からの蛍光測定を行う場合、そ
の都度試料保持体を洗浄して使用しなければならず、操
作が複雑になりまた非能率的である。When excited by light in the ultraviolet region, the material of the sample holder that holds the sample must generally be quartz glass. However, quartz glass is extremely expensive, cannot be made disposable, and when performing fluorescence measurement from many samples, the sample holder must be washed and used each time, which complicates the operation. It is inefficient.
本発明の目的は、励起光が紫外光である場合でも、安価
な、しかもディスポーザブルにできる蛍光測定用試料保
持体を提供することにある。An object of the present invention is to provide an inexpensive and disposable sample holder for fluorescence measurement, even when the excitation light is ultraviolet light.
上記目的は、励起光が直接プラスチックなどの部分を照
射しないように、その表面に金属膜または誘電体薄膜を
形成たり、または、照射される部分を金属箔や板などで
置き換えることにより達成される。The above object is achieved by forming a metal film or a dielectric thin film on the surface so that the excitation light does not directly irradiate a portion such as plastic, or by replacing the irradiated portion with a metal foil or a plate. .
プラスチックまたはガラス製の試料保持体の表面の少な
くとも一部を金属膜、誘電体薄膜または金属箔、金属板
などで覆ったり、また試料保持体の一部を金属で構成す
ることにより、励起光が金属面または誘電体薄膜面で反
射してガラスまたはプラスチックを直接励起せず、ガラ
スまたはプラスチックからの蛍光が生じなくなる。Excitation light can be generated by covering at least a part of the surface of a sample holder made of plastic or glass with a metal film, a dielectric thin film or a metal foil, a metal plate, etc., or by configuring a part of the sample holder with a metal. It does not directly excite the glass or plastic by reflection on the metal surface or the dielectric thin film surface, and fluorescence from the glass or plastic does not occur.
以下、本発明の実施例を示す。 Examples of the present invention will be shown below.
＜実施例１＞ 第１図は、断面の形状が凹状である試料保持体１の表面
の全面を、アルミニウムや銀やクロム等の金属膜または
誘電体多層膜２で被覆した場合の試料保持体の断面図で
ある。このように試料保持体は、例えば既知の真空蒸着
法に従って、市販のマイクロプレート（ポリスチレン製
など）の１個または複数のウェルの表面にアルミニウム
の膜を蒸着することによって得られる。このアルミニウ
ムを蒸着したマイクロプレートを使用してパラヒドロキ
シフェニルプロピオン酸（HPPA）二量体からの蛍光強度
を測定した結果を第５図に示す。発振波長が325nmのHe
−Cdレーザ光を使用し、種々の濃度に調製したHPPA二量
体（300μ）からの蛍光強度（測定蛍光波長:405nm）
を測定した。図中の曲線７はアルミニウムを蒸着しない
市販のマイクロプレートの場合であり、曲線６は蒸着に
よりウェルの表面にアルミニウム膜を形成したマイクロ
プレートの場合である。このようにアルミニウム蒸着膜
を施すことにより、マイクロプレート自体からの蛍光の
影響を受けず、より低濃度の蛍光体からの蛍光測定が可
能となった。Example 1 FIG. 1 shows a sample holder in which the entire surface of the sample holder 1 having a concave cross section is covered with a metal film of aluminum, silver, chromium or the like or a dielectric multilayer film 2. FIG. Thus, the sample holder can be obtained by depositing an aluminum film on the surface of one or more wells of a commercially available microplate (such as polystyrene) according to a known vacuum deposition method. FIG. 5 shows the result of measuring the fluorescence intensity from the para-hydroxyphenylpropionic acid (HPPA) dimer using this aluminum-deposited microplate. He with an oscillation wavelength of 325 nm
-Fluorescence intensity from HPPA dimer (300μ) prepared to various concentrations using Cd laser light (measured fluorescence wavelength: 405 nm)
Was measured. Curve 7 in the figure is the case of a commercially available microplate on which aluminum is not vapor deposited, and curve 6 is the case of a microplate in which an aluminum film is formed on the surface of the well by vapor deposition. By applying the aluminum vapor deposition film in this manner, it is possible to measure fluorescence from a phosphor having a lower concentration without being affected by fluorescence from the microplate itself.
本実施例では、マイクロプレートのウェルの表面全面に
アルミニウム蒸着膜を形成した。そのため、励起光の入
射角度は＋90度から−90度まで、つまり試料上方からで
あればほとんど水平方向からでも照射でき、また検出す
る蛍光の方向も試料上方であればどの方向でもかまわな
い。励起光および蛍光の光軸を、試料に対して種々の角
度に調整することができる。In this example, an aluminum vapor deposition film was formed on the entire surface of the well of the microplate. Therefore, the incident angle of the excitation light is from +90 degrees to −90 degrees, that is, the irradiation can be performed from almost the horizontal direction above the sample, and the fluorescence to be detected may be in any direction above the sample. The optical axes of the excitation light and the fluorescence can be adjusted to various angles with respect to the sample.
本実施例で形成した蒸着膜には膜の保護コーティングを
施していないため、繰り返しの使用には難があり、どち
らかといえばディスポーザブル的な使用法でその効果を
発揮する。繰り返し使用するような場合には、アルミニ
ウム蒸着膜の表面にさらに保護コーティング（例えばMg
F2膜）を施すことが望ましい。Since the vapor-deposited film formed in this example is not coated with a protective film, it is difficult to repeatedly use the film, and the effect is exhibited in a rather disposable method. In case of repeated use, a protective coating (eg Mg
It is desirable to apply a F 2 film).
また、本実施例では、被覆法としてアルミニウムの真空
蒸着法をもちいたが、クロムなどでも同様の効果が得ら
れる。また、真空蒸着法ばかりでなく既知のスパッタリ
ング法やニッケルなどの無電界メッキなどにより、形成
した金属膜でも同様の効果を得ることができる。さらに
金属ばかりでなく、マイクロプレートの表面に、例えば
325nm励起光を反射するような誘電体多層膜を蒸着によ
り形成された場合でもほぼ同様の効果が得られる。Further, in this embodiment, the vacuum deposition method of aluminum was used as the coating method, but the same effect can be obtained with chromium or the like. Further, not only the vacuum vapor deposition method but also the known sputtering method, electroless plating of nickel or the like, can be used to obtain the same effect with a metal film formed. In addition to metal, on the surface of the microplate, for example,
Similar effects can be obtained even when a dielectric multilayer film that reflects 325 nm excitation light is formed by vapor deposition.
＜実施例２＞ 第２図は、断面の形状が凹状である試料保持体１の表面
の底面部分を、アルミニウムや銀やクロム等の金属膜ま
たは誘電体多層膜２で被覆した場合の試料保持体の断面
図である。例えば、実施例１においてウェルの側面をマ
スクして底面のみにアルムニウム蒸着膜を形成したマイ
クロプレートで実施することができる。蛍光測定装置で
励起光がほぼ鉛直方向よりウェル内の測定試料に照射さ
れる場合、励起光は底面にのみ照射され側面には照射さ
れない。このような場合は、底面部のみに金属膜または
誘電体多層膜を形成しただけで実施例１とほぼ同じ効果
を得ることができる。<Embodiment 2> FIG. 2 shows sample holding when the bottom surface of the surface of the sample holder 1 having a concave cross section is covered with a metal film such as aluminum, silver, or chromium, or a dielectric multilayer film 2. It is sectional drawing of a body. For example, it can be carried out by using a microplate in which the side surface of the well is masked and the aluminum vapor deposition film is formed only on the bottom surface in Example 1. When the excitation light is applied to the measurement sample in the well from the vertical direction in the fluorescence measurement device, the excitation light is applied only to the bottom surface and not to the side surface. In such a case, substantially the same effect as that of the first embodiment can be obtained by forming the metal film or the dielectric multilayer film only on the bottom surface.
＜実施例３＞ 第３図は、断面の形状が凹状である試料保持体１の表面
の底面部分に、その部分とほぼ同じ形状の金属箔または
板３を配置した場合の試料保持体の断面図である。金属
箔としてはアルミニウム箔や銀箔（厚さ10〜100μ
ｍ）、金属板としてはアルミニウム板やステンレス板
（厚さ0.1〜2mm程度）などが適当である。市販のマイク
ロプレート（ウェル径が約6.4mm）を使用し、約6mm径の
アルミニウム箔（厚さ100μｍ）をウェルの表面の底面
部に接着させて試料保持体を作製した。接着にはエポキ
シ系接着剤を使用した。この試料保持体を使用してHPPA
二量体からの蛍光強度の測定を行った結果、実施例１と
ほぼ同じ効果が得られた。本例ではアルミニウム箔を接
着剤によりウェルの表面の底面部に固定したが、金属板
等が十分に重く、測定試料を試料保持体に注入したとき
に金属板等が浮き上がらなければ接着する必要はない。<Example 3> Fig. 3 is a cross section of a sample holder in which a metal foil or plate 3 having substantially the same shape as that of the bottom surface of the surface of the sample holder 1 having a concave cross section is arranged. It is a figure. Aluminum foil or silver foil (thickness 10 ~ 100μ
m), an aluminum plate or a stainless plate (thickness of about 0.1 to 2 mm) is suitable as the metal plate. A commercially available microplate (well diameter: about 6.4 mm) was used, and an aluminum foil (thickness: 100 μm) having a diameter of about 6 mm was adhered to the bottom surface of the well to prepare a sample holder. An epoxy adhesive was used for adhesion. HPPA using this sample holder
As a result of measuring the fluorescence intensity from the dimer, almost the same effect as in Example 1 was obtained. In this example, the aluminum foil was fixed to the bottom surface of the well surface with an adhesive, but if the metal plate or the like is sufficiently heavy and the metal plate or the like does not float when the measurement sample is injected into the sample holder, then it is not necessary to bond it. Absent.
＜実施例４＞ 第４図は、板状の金属板４の上にプラスチックまたはガ
ラス５を接着して凹状の断面を形成した試料保持体の断
面図である。1mmの厚さのアルミニウム板の上に、内径6
mm、外径8mm、長さ10mmのポリスチレン製の円筒を接着
して試料保持体を作製した。この試料保持体を使用して
HPPA二量体からの蛍光強度の測定を行った結果、実施例
１とほぼ同じ効果が得られた。また、同じように6mm径
の孔を作製した3mmのの厚さの黒色アクリル板をアルミ
ニウム板上に接着して得た試料保持体でも同様の効果が
得られた。本例では金属板としてアルミニウム板を使用
したがステンレス板などでも作製できる。さらに試料保
持体を作製した後、既知の真空蒸着法またはメッキ等に
より試料保持体の表面を処理すれば、より効果が大きく
なる。<Example 4> FIG. 4 is a cross-sectional view of a sample holder in which a plastic or glass 5 is bonded onto a plate-shaped metal plate 4 to form a concave cross-section. 6mm inner diameter on a 1mm thick aluminum plate
A cylinder made of polystyrene having a diameter of 8 mm, an outer diameter of 8 mm and a length of 10 mm was adhered to produce a sample holder. Using this sample holder
As a result of measuring the fluorescence intensity from the HPPA dimer, almost the same effect as in Example 1 was obtained. The same effect was also obtained with a sample holder obtained by adhering a black acrylic plate having a thickness of 3 mm and similarly having a hole of 6 mm diameter to an aluminum plate. In this example, an aluminum plate was used as the metal plate, but a stainless plate or the like can also be used. Further, if the surface of the sample holder is treated by a known vacuum deposition method or plating after the sample holder is manufactured, the effect is further enhanced.
上記実施例１ないし４では、試料保持体の断面の形状が
凹状の場合を示したが、これに限定されるわけではな
く、例えばＶ字状やＵ字状などの断面形状の試料保持体
にも適用できる。In Examples 1 to 4 described above, the case where the cross section of the sample holder has a concave shape is shown, but the present invention is not limited to this, and for example, a sample holder having a V-shaped or U-shaped cross-sectional shape may be used. Can also be applied.
上記実施例１ないし４において、膜、箔、板等の反射体
の表面を鏡面状に仕上げれば、反射体部分での励起光の
散乱を減少させることができるので、蛍光測定が容易に
なる。また、この場合、反射体面で励起光が反射される
ため、実質的に励起光強度が２倍になり、蛍光強度も増
大するという効果もある。また、本発明の試料保持体
は、励起波長が紫外領域のときばかりでなく可視または
赤外領域などの場合にも使用でき、同様の効果がある。In Examples 1 to 4, if the surface of a reflector such as a film, a foil, or a plate is mirror-finished, it is possible to reduce the scattering of excitation light at the reflector, which facilitates fluorescence measurement. . Further, in this case, since the excitation light is reflected by the reflector surface, the excitation light intensity is substantially doubled, and the fluorescence intensity is also increased. Further, the sample holder of the present invention can be used not only when the excitation wavelength is in the ultraviolet region but also when it is in the visible or infrared region and has the same effect.
以上の実施例に示したように、市販のマイクロプレート
のウェルの表面の１部または全面を金属等の反射体で覆
うことにより、励起光が紫外光の場合でも適用できる蛍
光測定用の試料保持体を作製することができる。この蛍
光測定用の試料保持体は市販のマイクロプレートの形状
そのままであるため、市販のマイクロプレートを使用し
て蛍光測定等を行う装置において、光学系の配置及び試
料台の寸法などを変更することなく適用することがで
き、しかもより高感度が測定が可能となる。As shown in the above examples, by covering a part or the whole surface of the well of a commercially available microplate with a reflector such as a metal, sample holding for fluorescence measurement applicable even when the excitation light is ultraviolet light The body can be made. Since this sample holder for fluorescence measurement has the same shape as the commercially available microplate, it is necessary to change the arrangement of the optical system and the size of the sample table in the device for performing fluorescence measurement using the commercially available microplate. It can be applied without any need, and higher sensitivity can be measured.
本発明によれば、励起光が紫外光であっても、高価な石
英ガラスで試料保持体を作製することなく、プラスチッ
クやガラスと金属または誘電体多層膜で試料保持体を作
製することができ、比較的安価に蛍光測定用の試料保持
体を得ることができる。また、ひいてはディスポーザブ
ル試料保持体として使用することができる。According to the present invention, even if the excitation light is ultraviolet light, a sample holder can be made of plastic or glass and metal or a dielectric multilayer film without making the sample holder of expensive quartz glass. Thus, the sample holder for fluorescence measurement can be obtained relatively inexpensively. Further, it can be used as a disposable sample holder.
第１図、第２図、第３図および第４図はそれぞれ、本発
明の実施例によって得られる蛍光測定用試料保持体の正
面からみた断面図、第５図は本発明の実施例によって得
られる蛍光強度対濃度特性の改善を説明する図である。 １……プラスチックまたはガラス製の試料保持体 ２……金属膜または誘電体多層膜 ３……金属箔または金属板 ４……金属板 ５……プラスチックまたはガラス ６……ウェルの表面にアルミニウム膜を形成したマイク
ロプレートを使用した場合の蛍光強度対濃度特性曲線 ７……市販のマイクロプレートを使用した場合の蛍光強
度対濃度特性曲線。1, 2, 3, and 4 are cross-sectional views of the sample holder for fluorescence measurement obtained from the embodiment of the present invention as seen from the front, and FIG. 5 is obtained from the embodiment of the present invention. It is a figure explaining the improvement of the fluorescence intensity-concentration characteristic which is made. 1 ... Plastic or glass sample holder 2 ... Metal film or dielectric multilayer film 3 ... Metal foil or metal plate 4 ... Metal plate 5 ... Plastic or glass 6 ... Aluminum film on well surface Fluorescence intensity-concentration characteristic curve when using the formed microplate 7 ... Fluorescence intensity-concentration characteristic curve when using a commercially available microplate.
Claims (3)
持体であつて、上方から励起光を前記蛍光測定用試料保
持体に保持された試料に照射し、前記試料から上方に発
する蛍光を測定する蛍光測定装置に使用する蛍光測定用
試料保持体において、貫通する中空部分を有するプラス
チツクまたはガラス部材を金属板の上に接着して、前記
の凹状の部分を形成することを特徴とする蛍光測定用試
料保持体。1. A sample holder for fluorescence measurement having a concave cross-section, wherein fluorescence is emitted upward from the sample by irradiating the sample held on the sample holder for fluorescence measurement with excitation light from above. In a sample holder for fluorescence measurement used in a fluorescence measurement device for measuring, a plastic or glass member having a hollow portion penetrating is adhered onto a metal plate to form the concave portion. Sample holder for fluorescence measurement.
テンレス板であることを特徴とする特許請求の範囲第１
項に記載の蛍光測定用試料保持体。2. The metal plate is an aluminum plate or a stainless steel plate.
Item 6. A sample holder for fluorescence measurement according to the item.
ことを特徴とする特許請求の範囲第１項に記載の蛍光測
定用試料保持体。3. The sample holder for fluorescence measurement according to claim 1, wherein the surface of the metal plate is formed into a mirror surface.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63216270A JPH0695073B2 (en) | 1988-09-01 | 1988-09-01 | Sample holder for fluorescence measurement |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63216270A JPH0695073B2 (en) | 1988-09-01 | 1988-09-01 | Sample holder for fluorescence measurement |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0266430A JPH0266430A (en) | 1990-03-06 |
| JPH0695073B2 true JPH0695073B2 (en) | 1994-11-24 |
Family
ID=16685916
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP63216270A Expired - Lifetime JPH0695073B2 (en) | 1988-09-01 | 1988-09-01 | Sample holder for fluorescence measurement |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0695073B2 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004109107A (en) * | 2002-07-25 | 2004-04-08 | Nippon Sheet Glass Co Ltd | Vessel for biochemistry |
| JP2006276000A (en) * | 2005-03-04 | 2006-10-12 | Hitachi High-Technologies Corp | Fluorescent molecule measurement system |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19712484C2 (en) | 1997-03-25 | 1999-07-08 | Greiner Gmbh | Microplate with transparent bottom and process for its production |
| GB0122286D0 (en) * | 2001-09-14 | 2001-11-07 | Scient Generics Ltd | Optical coatings for high-throughput laboratory consumables |
| EP1646862B1 (en) * | 2003-06-03 | 2018-04-11 | Siemens Healthcare Diagnostics Inc. | Automatic identification of reagent test strips using reflectance values |
| JP4701739B2 (en) * | 2005-02-17 | 2011-06-15 | パナソニック株式会社 | Fluorescence measuring device |
| JP4741855B2 (en) * | 2005-02-18 | 2011-08-10 | カシオ計算機株式会社 | Biopolymer analysis chip, analysis support apparatus, and biopolymer analysis method |
| KR101183159B1 (en) | 2008-12-22 | 2012-09-17 | 한국전자통신연구원 | Biochip and apparatus for detecting bio materials by using the biochip |
| JP2011038922A (en) * | 2009-08-12 | 2011-02-24 | Sony Corp | Light detection chip, and light detection device using the same |
| JP6017107B2 (en) * | 2009-12-28 | 2016-10-26 | ソニー株式会社 | Image sensor, manufacturing method thereof, and sensor device |
| WO2024226850A1 (en) * | 2023-04-25 | 2024-10-31 | Rensselaer Polytechnic Institute | Systems and methods for tissue staining |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES8501998A1 (en) * | 1982-10-12 | 1984-12-16 | Dynatech Lab | Non-fluorescent vessels for holding test samples in fluorescent assays. |
| JPS6266141A (en) * | 1985-09-19 | 1987-03-25 | Sumitomo Bakelite Co Ltd | Container for fluorescence immunoassay |
-
1988
- 1988-09-01 JP JP63216270A patent/JPH0695073B2/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004109107A (en) * | 2002-07-25 | 2004-04-08 | Nippon Sheet Glass Co Ltd | Vessel for biochemistry |
| JP2006276000A (en) * | 2005-03-04 | 2006-10-12 | Hitachi High-Technologies Corp | Fluorescent molecule measurement system |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0266430A (en) | 1990-03-06 |
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| EXPY | Cancellation because of completion of term |