JPH07102142B2 - Method for producing red pigment by cultured statice cells - Google Patents
Method for producing red pigment by cultured statice cellsInfo
- Publication number
- JPH07102142B2 JPH07102142B2 JP10464490A JP10464490A JPH07102142B2 JP H07102142 B2 JPH07102142 B2 JP H07102142B2 JP 10464490 A JP10464490 A JP 10464490A JP 10464490 A JP10464490 A JP 10464490A JP H07102142 B2 JPH07102142 B2 JP H07102142B2
- Authority
- JP
- Japan
- Prior art keywords
- statice
- red pigment
- cultured
- cells
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000001054 red pigment Substances 0.000 title claims description 15
- 238000004519 manufacturing process Methods 0.000 title claims description 5
- 240000001585 Limonium sinuatum Species 0.000 title 1
- 241000201282 Limonium Species 0.000 claims description 19
- 239000007788 liquid Substances 0.000 claims description 12
- 238000012258 culturing Methods 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 6
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims description 5
- 229930192334 Auxin Natural products 0.000 claims description 4
- 239000002363 auxin Substances 0.000 claims description 4
- 239000002609 medium Substances 0.000 description 20
- 210000004027 cell Anatomy 0.000 description 8
- 210000004748 cultured cell Anatomy 0.000 description 5
- 239000005971 1-naphthylacetic acid Substances 0.000 description 3
- IIDAJRNSZSFFCB-UHFFFAOYSA-N 4-amino-5-methoxy-2-methylbenzenesulfonamide Chemical compound COC1=CC(S(N)(=O)=O)=C(C)C=C1N IIDAJRNSZSFFCB-UHFFFAOYSA-N 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 206010020649 Hyperkeratosis Diseases 0.000 description 3
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 description 3
- 241000316733 Limonium latifolium Species 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 3
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 description 3
- 229960001669 kinetin Drugs 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- 239000005972 6-Benzyladenine Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- 241000530839 Goniolimon tataricum Species 0.000 description 2
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 239000000049 pigment Substances 0.000 description 2
- SMYMJHWAQXWPDB-UHFFFAOYSA-N (2,4,5-trichlorophenoxy)acetic acid Chemical compound OC(=O)COC1=CC(Cl)=C(Cl)C=C1Cl SMYMJHWAQXWPDB-UHFFFAOYSA-N 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- 239000003559 2,4,5-trichlorophenoxyacetic acid Substances 0.000 description 1
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 1
- HXKWSTRRCHTUEC-UHFFFAOYSA-N 2,4-Dichlorophenoxyaceticacid Chemical compound OC(=O)C(Cl)OC1=CC=C(Cl)C=C1 HXKWSTRRCHTUEC-UHFFFAOYSA-N 0.000 description 1
- GOSWTRUMMSCNCW-HNNGNKQASA-N 9-ribosyl-trans-zeatin Chemical compound C1=NC=2C(NC\C=C(CO)/C)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O GOSWTRUMMSCNCW-HNNGNKQASA-N 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 244000020518 Carthamus tinctorius Species 0.000 description 1
- 235000003255 Carthamus tinctorius Nutrition 0.000 description 1
- XXFACTAYGKKOQB-SSDOTTSWSA-N Dihydrozeatin Natural products OC[C@H](C)CCNC1=NC=NC2=C1NC=N2 XXFACTAYGKKOQB-SSDOTTSWSA-N 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 101000588230 Homo sapiens N-alpha-acetyltransferase 10 Proteins 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- MKYBYDHXWVHEJW-UHFFFAOYSA-N N-[1-oxo-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propan-2-yl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(C(C)NC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 MKYBYDHXWVHEJW-UHFFFAOYSA-N 0.000 description 1
- 102100031641 N-alpha-acetyltransferase 10 Human genes 0.000 description 1
- 229930182559 Natural dye Natural products 0.000 description 1
- 235000005205 Pinus Nutrition 0.000 description 1
- 241000218602 Pinus <genus> Species 0.000 description 1
- 241001290145 Psylliostachys suworowii Species 0.000 description 1
- MUPFEKGTMRGPLJ-RMMQSMQOSA-N Raffinose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 MUPFEKGTMRGPLJ-RMMQSMQOSA-N 0.000 description 1
- MUPFEKGTMRGPLJ-UHFFFAOYSA-N UNPD196149 Natural products OC1C(O)C(CO)OC1(CO)OC1C(O)C(O)C(O)C(COC2C(C(O)C(O)C(CO)O2)O)O1 MUPFEKGTMRGPLJ-UHFFFAOYSA-N 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- XXFACTAYGKKOQB-ZETCQYMHSA-N dihydrozeatin Chemical compound OC[C@@H](C)CCNC1=NC=NC2=C1NC=N2 XXFACTAYGKKOQB-ZETCQYMHSA-N 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000576 food coloring agent Substances 0.000 description 1
- 235000002864 food coloring agent Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 239000003617 indole-3-acetic acid Substances 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000000978 natural dye Substances 0.000 description 1
- 150000002972 pentoses Chemical class 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- MUPFEKGTMRGPLJ-ZQSKZDJDSA-N raffinose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)O1 MUPFEKGTMRGPLJ-ZQSKZDJDSA-N 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 239000000979 synthetic dye Substances 0.000 description 1
- UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 description 1
- UZKQTCBAMSWPJD-FARCUNLSSA-N trans-zeatin Chemical compound OCC(/C)=C/CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-FARCUNLSSA-N 0.000 description 1
- 229940023877 zeatin Drugs 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Description
【発明の詳細な説明】 〔産業上の利用分野〕 本発明はスターチス培養細胞による赤色色素の生産法に
関する。DETAILED DESCRIPTION OF THE INVENTION [Industrial field of use] The present invention relates to a method for producing a red pigment by a statice culture cell.
近年、食品用着色剤として合成色素より安全性の高い天
然色素の使用が望まれている。天然色素は植物等から抽
出することにより製造されているが、栽培のために広大
な土地、時間および労力が必要であり、また収穫は天
候、土壌等に左右され、安定した品質の製品を得ること
が困難である。従来、ベニバナ培養細胞等の植物から赤
色色素を生産する方法は知られているが、イソマツ科、
イソマス属のスターチス（Limonium Latifolium O.Kunt
ze）を用いた赤色色素の生産法はまだ知られていない。In recent years, it has been desired to use natural dyes, which are safer than synthetic dyes, as food coloring agents. Natural pigments are produced by extracting them from plants, etc., but they require vast land, time and labor for cultivation, and the harvest depends on the weather, soil, etc. to obtain stable quality products. Is difficult. Conventionally, a method of producing a red pigment from plants such as safflower cultured cells is known,
Statice of the genus Isomus (Limonium Latifolium O. Kunt)
The method for producing red pigment using ze) is not yet known.
本発明者らは、特願平１−275538号（特開平３−139287
号公報）でスターチスの葉または茎の培養細胞から赤色
色素を白色光下で効率よく生産する方法を提案してい
る。The present inventors have filed Japanese Patent Application No. 1-275538 (Japanese Patent Application Laid-Open No. 3-139287).
(Japanese Patent Publication), a method for efficiently producing a red pigment from cultured leaves or stem cells of statice under white light is proposed.
本発明の目的は、スターチスの培養細胞から安定かつ一
定品質の赤色色素をさらに効率よく生産することができ
る方法を提供することにある。It is an object of the present invention to provide a method capable of producing a stable and constant quality red pigment from cultured cells of statice more efficiently.
本発明は、スターチスの赤色色素を生産するスターチス
培養細胞を、オーキシンおよびサイトカニンを含む液体
培地を用いて白色光下で培養するに際し、該液体培地の
pHを７〜４とすることを特徴とするスターチス培養細胞
による赤色色素の生産法に関する。The present invention provides a method of culturing, in white light, a liquid medium containing auxin and cytocanin, which is used for culturing a statice cultured cell producing a red pigment of statice, in the liquid medium.
The present invention relates to a method for producing a red pigment by culture of statice cells, which has a pH of 7 to 4.
本発明に用いられるスターチスは、イソマツ属に属する
塩生植物であり、通常園芸種として栽培される、リモニ
ウム・ツヌアーツム（L.sinuatumMill.）やリモニウム
・スウォロウィー（L.suworowii O.Kuntze）などの一年
草、リモニウム・ラティフォリウム（L.latifolium O.K
untze）やリモニウム・タータリクム（L.tataricum Mil
l.）などの多年草が用いられる。The statice used in the present invention is a halophyte belonging to the genus Pinus, which is usually cultivated as a horticultural species, and is one year such as limonium tunuatum (L.sinuatumMill.) And limonium swallowowie (L.suworowii O.Kuntze). Grass, limonium latifolium (L.latifolium OK
untze) and limonium tartaricum (L.tataricum Mil
Perennial plants such as l.) are used.
本発明に用いられるスターチス培養細胞は、スターチス
組織から誘導されるカスルを液体培養して得られる。該
カルス誘導のために用いられるスターチス組織としては
葉または茎の組織が用いられる。The statice culture cells used in the present invention can be obtained by liquid-culturing Casul derived from statice tissue. As the statice tissue used for the callus induction, leaf or stem tissue is used.
スターチス培養細胞を培養する培地としては、公知のMu
rashige−Skoog培地（1962）、Linsmaier−Skoog培地
（1965）、White培地（1963）、Gemorg B−５（196
8）、Nitsch培地（1951）、Heller培地（1953）、Schen
k−Hildebrandt培地（1972）、Nitsch−Nitsch培地（19
67）、Kohlenbach−Schmidt培地（1975）、Knop培地（1
865）またはこれらの改変培地を用いることができる。
赤色色素の培養に際し、これらの培地に炭素源としてシ
ュークロース、グルコース、ラフィノース、フラクトー
ス、ペントース、マルトースなど、植物ホルモンとして
オーキシン、サイトカニンなどが添加される。オーキシ
ンとしてはインドール−３−酢酸（1AA）、１−ナフタ
レン酢酸（NAA）、2,4−ジクロロフェノキシ酢酸（2,4
−Ｄ）、2,4,5−トリクロロフェノキシ酢酸（2,4,5−
Ｔ）が挙げられ、サイトカニンとしてはゼアチン、ジヒ
ドロゼアチン、リボシルゼアチン、６−ベンジルアデニ
ン（BA）、カイネチンなどが挙げられる。As a medium for culturing statice culture cells, known Mu
rashige-Skoog medium (1962), Linsmaier-Skoog medium (1965), White medium (1963), Gemorg B-5 (196
8), Nitsch medium (1951), Heller medium (1953), Schen
k-Hildebrandt medium (1972), Nitsch-Nitsch medium (19
67), Kohlenbach-Schmidt medium (1975), Knop medium (1
865) or modified media thereof can be used.
Upon culturing the red pigment, sucrose, glucose, raffinose, fructose, pentose, maltose and the like as carbon sources and auxin, cytocanin and the like as plant hormones are added to these media. As auxins, indole-3-acetic acid (1AA), 1-naphthalene acetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4
-D), 2,4,5-trichlorophenoxyacetic acid (2,4,5-
T), and examples of cytocanins include zeatin, dihydrozeatin, ribosylzeatin, 6-benzyladenine (BA), and kinetin.
本発明においては、スターチス培養細胞による赤色色素
の生産を効率よく行うために、上記液体培地のpHを７〜
４とする必要がある。好ましいpHは５〜４の範囲であ
る。また培養に際しては100〜20,000luxの白色光を照射
するのが好ましく、より好ましくは2000lux程度であ
る。この白色光としては、例えば蛍光灯、白色電球など
の光を用いることができる。また培養条件には特に限定
はないが、25℃前後の温度で液体培地を振とうまたは回
転培養するのが好ましい。In the present invention, the pH of the liquid medium is 7 to 7 in order to efficiently produce the red pigment by the statice cultured cells.
It must be 4. The preferred pH is in the range of 5-4. Further, during the culture, it is preferable to irradiate 100 to 20,000 lux of white light, more preferably about 2000 lux. As the white light, for example, light from a fluorescent lamp or a white light bulb can be used. The culture conditions are not particularly limited, but it is preferable to shake or spin culture the liquid medium at a temperature of around 25 ° C.
以下、本発明を実施例により詳しく説明する。実施例１
〜４および比較例１、２ カイネチン10-5M、NAA10-5M、シュークロース３％およ
びAger（寒天）１％を含むMurashige−Skoog寒天培地上
に、スターチスの葉および茎を置床し、約2000luxの白
色光下で培養し、赤色を呈するカルスを誘導した。この
カルスを同様の液体培地に移植し、約5000luxの白色光
下で培養して多数の赤色を呈する細胞集塊を得た。Hereinafter, the present invention will be described in detail with reference to Examples. Example 1
-4 and Comparative Examples 1 and 2, statice leaves and stems were placed on a Murashige-Skoog agar medium containing kinetin 10 -5 M, NAA10 -5 M, sucrose 3% and Ager (agar) 1%, and After culturing under 2000 lux of white light, red callus was induced. The callus was transplanted to the same liquid medium and cultured under white light of about 5000 lux to obtain a large number of red cell aggregates.
この細胞集塊を、それぞれpH3、４、５、６、７、８に
調製したカイネチン10-5M、NAA10-5およびシュークロー
ス３％を含むMurashige−Skoog培置にそれぞれ接種し
た。細胞集塊の接種量は液体培地30mlに対して1gの割合
とした。次にこれを約5000luxの白色光下で10日間培養
を行い、それぞれのpHにおける白色含有量を調べ、結果
を第１表に示した。色素含有量は、1gの細胞集塊から１
％HClを含むメタノールにより色素を抽出し、530nmにお
ける吸光度を測定して算出した。The cell clumps were inoculated into Murashige-Skoog culture medium containing kinetin 10 -5 M, NAA 10 -5 and sucrose 3% adjusted to pH 3, 4, 5 , 6, 7, and 8 respectively. The inoculation amount of the cell clumps was 1 g per 30 ml of the liquid medium. Next, this was cultured under white light of about 5000 lux for 10 days, the white content at each pH was examined, and the results are shown in Table 1. Pigment content is 1 from 1g of cell clumps
The dye was extracted with methanol containing% HCl, and the absorbance at 530 nm was measured and calculated.
第１表から、液体培地をpH7〜４に調整して用いること
により、赤色色素の生産が効率よく行われることが示さ
れる。 From Table 1, it is shown that the red pigment is efficiently produced by adjusting the pH of the liquid medium to 7 to 4.
実施例５、６および比較例３ 実施例１において、それぞれpH4、６、８の液体培地を
用いて培養した以外は実施例１と同様にして培養を行
い、10日後の色素含有量を実施例１と同様の方法で調 べ、その結果を第２表に示した。Examples 5 and 6 and Comparative Example 3 Cultivation was performed in the same manner as in Example 1 except that the liquid mediums of pH 4, 6, and 8 were used in Example 1, respectively, and the dye content after 10 days was determined as in Example 1. The same procedure as in 1 was conducted, and the results are shown in Table 2.
第２表からも本発明の液体培地を用いることにより効率
よく赤色色素が生産されることが示される。 Table 2 also shows that the red pigment is efficiently produced by using the liquid medium of the present invention.
本発明によれば、スターチス培養細胞をpH7〜４の範囲
に調整した液体培地で培養することにより、安定に効率
よく赤色色素を生産することができる。According to the present invention, the red pigment can be stably and efficiently produced by culturing the statice cultured cells in the liquid medium adjusted to the pH range of 7 to 4.
Claims (1)
ス培養細胞を、オーキシンおよびサイトカニンを含む液
体培地を用いて白色光下で培養するに際し、該液体培地
のpHを７〜４とすることを特徴とするスターチス培養細
胞による赤色色素の生産法。1. A method for culturing a statice culture cell producing a red pigment of statice in a liquid medium containing auxin and cytocanine under white light, wherein the pH of the liquid medium is 7 to 4. A method for producing a red pigment by cultured Statis cells.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10464490A JPH07102142B2 (en) | 1990-04-20 | 1990-04-20 | Method for producing red pigment by cultured statice cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10464490A JPH07102142B2 (en) | 1990-04-20 | 1990-04-20 | Method for producing red pigment by cultured statice cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH044885A JPH044885A (en) | 1992-01-09 |
| JPH07102142B2 true JPH07102142B2 (en) | 1995-11-08 |
Family
ID=14386164
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP10464490A Expired - Lifetime JPH07102142B2 (en) | 1990-04-20 | 1990-04-20 | Method for producing red pigment by cultured statice cells |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH07102142B2 (en) |
-
1990
- 1990-04-20 JP JP10464490A patent/JPH07102142B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPH044885A (en) | 1992-01-09 |
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