JPH0710229B2 - Method for growing soil microorganisms - Google Patents

Description

Detailed Description of the Invention

[0001]

This invention relates to the use of soil microorganisms as a deodorant by utilizing the characteristics of the soil microorganisms. It can be doubled to enhance the deodorizing effect, extend the period of use of the deodorant, and obtain the necessary deodorizing effect with a small amount of the deodorant used. The present invention relates to a method for growing soil microorganisms capable of producing soil microorganisms.

[0002]

2. Description of the Related Art Conventionally, as a deodorant for removing a malodor, for example, an adsorption type deodorant utilizing activated carbon that physically adsorbs a malodorous component, and chemically reacting with the malodorous component to supplement the malodorous component. Chemical deodorants or biological deodorants that utilize the assimilation action against the malodor of microorganisms are known.

[0003] Of these, the one utilizing the physical adsorption force of activated carbon or the like cannot expect any further effect when the adsorption of the malodorous component is saturated, and it is necessary to use a new deodorizing deodorant each time. Further, although chemical deodorization is quick-acting, it is temporary and is not effective for a long period of time, and there is a problem such as environmental pollution depending on what is used.

In contrast, biological deodorants are expected to be fast acting and permanent. And as an invention for extracting soil microorganisms as a deodorant, for example, Japanese Patent Laid-Open No. 62-297288 (“Method for fertilizing offensive odor gas from a sewage treatment plant such as livestock manure, human waste, sewage and fish ilium with a humus deodorant” )) Etc. have been published.

[0005]

The number of microorganisms contained in 1 g of soil has an upper limit depending on the type of soil and microorganisms. Therefore, when microorganisms are extracted from soil, the maximum number of microorganisms is limited. There is.

On the other hand, the deodorizing effect is proportional to the number of microorganisms to some extent, and the amount of the deodorant used is also proportional to the number of microorganisms.
Further, since the period of use of the deodorant is considered to be proportional to the number of microorganisms to some extent, it is desirable that the number of microorganisms contained in the deodorant be as large as possible.

Further, in order to generate soil microorganisms, it is necessary to extract soil microorganisms from the humus, and for this purpose, it is necessary to secure a humus containing soil microorganisms each time. There are also inconveniences such as troublesome acquisition work.

The present invention was made in view of the above problems and was devised to solve the problems. The object of the present invention is to utilize the characteristics of soil microorganisms as a deodorant. In this case, the soil microbes used in the deodorant are grown to a number of thousands to tens of thousands of times the number contained in natural soil to enhance the deodorizing effect, and the deodorant is used for a longer period of time, and further, a small amount of deodorant is used. It is an object of the present invention to provide a method for proliferating soil microorganisms, which can obtain the required deodorizing effect with the use amount of the agent and can reproduce the soil microorganisms inexhaustibly to produce the soil microorganisms.

[0009]

To achieve the above object, the invention according to claim 1 provides a soil microorganism extract obtained by mixing humus containing soil microorganisms with water and filtering the mixture. An alkaline aqueous solution is added to adjust the pH to precipitate iron ions and the like in the soil microbial extract, the precipitate is removed by filtration, and the sodium alginate aqueous solution is added to this to stir and mix the soil in the sol state. A microbial extract is made, and this sol-state soil microbial extract is dripped from the pores into an aqueous solution containing divalent and trivalent metal ions to retain the gel on the inside of the bead-like gelled surface. The bead-shaped soil microorganisms retained inside the gel are left in a medium for growth containing a divalent or trivalent metal ion, and the bead-like soil microorganisms that have been gelled to the inside How to increase the concentration It is Li Cheng thing.

Further, the invention according to claim 2 is that the soil microorganisms retained inside the gelled bead-like interior of claim 1 are left in the growth medium to allow the soil microorganisms in the beads to grow in the growth medium. To increase the concentration of soil microorganisms in the growth medium.

[0011]

The present invention having the above-described structure operates as follows. That is, according to the invention of claim 1, since the gelled bead-shaped soil microorganisms retained inside are grown several thousand to tens of thousands times, the grown bead-shaped soil microorganisms are deodorized. When used as a product, the number of soil microorganisms is several thousand to tens of thousands times higher than that of non-grown ones, increasing the deodorizing effect and extending the period of use of the deodorizing agent, and further reducing deodorization. It acts so that the required deodorizing effect can be obtained with the amount of the agent used.

According to the second aspect of the present invention, since the soil microorganisms propagate in the medium for growth that grows the soil microorganisms retained inside the gelled bead, the medium for growth is propagated. The soil microorganisms can be used to propagate the gel microorganisms that are retained inside the gelled bead, so that the soil microorganisms can be extracted from the humus only for the first time, and then this can be repeated indefinitely. It acts so as to be able to produce soil microorganisms that are the raw material of.

[0013]

EXAMPLES The present invention will be described in more detail based on the following examples.

[First Example] Humus (humic acid) containing soil microorganisms and water were mixed at a weight ratio of 1: 2 by adding water to the humus. In this case, the water used is preferably pure water that has been ion-exchanged after distillation.

Then, the humus and water are thoroughly mixed, and after 1 day, the mixed solution of humus and water is filtered. Filtration is carried out twice, the first time is rough filtration using gauze or the like to remove large particles of soil, and the second time is the mixed solution filtered by rough filtration with filter paper 1
Filter using seeds to obtain a soil microbial extract. This soil microbial extract exhibits an acidity of pH 2-3.

Since iron ions are dissolved in the soil microorganism extract, it is necessary to remove the iron ions.
Therefore, the diluted sodium hydroxide solution is mixed with the soil microbial extract to adjust the pH so that the pH becomes substantially neutral. By mixing this dilute sodium hydroxide solution, iron ions and the like precipitate.

When iron ions and the like are precipitated, the soil microorganism extract is filtered with one kind of filter paper to remove precipitates such as iron ions to obtain a neutralized soil microorganism extract. This neutralized soil microorganism extract contains 10 ⁶ to 10 ⁷ soil microorganisms per 1 g.

Next, the neutralized soil microbial extract is mixed with, for example, the same volume of a 4% aqueous solution of sodium alginate, and the mixture is sufficiently stirred and mixed so as to be completely homogeneous, and 2% sodium alginate neutralized. A soil microbial extract is obtained.
This sodium alginate / neutralized soil microorganism extract is in a sol state.

Then, the sol-state sodium alginate / neutralized soil microorganism extract is added dropwise from the pores to, for example, a 2% calcium chloride aqueous solution. In this case, for example, aluminum chloride may be used instead of the calcium chloride aqueous solution, and the point is that it is a solution containing divalent to trivalent metal ions.
The concentration of the solution is preferably 2% or more.

The surface of the sol-state sodium alginate / neutralized soil microbial extract dropped into the aqueous solution of calcium chloride reacts with the aqueous solution of calcium chloride, and the surface thereof becomes a semipermeable membrane of calcium alginate and is gelled.
In this way, the soil microorganisms extract is in the form of beads covered with semipermeable membrane calcium alginate whose entire surface is gelled in the calcium chloride aqueous solution, and the soil microorganisms are retained inside the beads.

In the calcium chloride aqueous solution, a bead-like substance in which soil microorganisms are retained inside a large number of drops produced is obtained. The large number of beads are taken out from the calcium chloride aqueous solution. For example, a colander is used for taking out, and after taking out, the calcium chloride on the surface of the beads is washed away by washing with water. This washing operation may be omitted if necessary.

1 is contained in the beads thus produced.
Although 10 ⁶ to 10 ⁷ soil microorganisms are present per g, the number of soil microorganisms contained in the beads is further increased to several thousand to several ten thousand times in order to enhance the function of the soil microorganisms.

In addition, at this time, when the beads for retaining soil microorganisms inside are directly used as a deodorant, etc.,
In order to prevent the membrane on the bead surface from breaking and the soil microorganisms inside to flow out, gelation is performed inside the beads. For this reason, the beads in which soil microorganisms are retained inside are left to proliferate in a growth medium containing a divalent to trivalent metal ion.

The growth medium is, for example, sugar and yeast extract,
It consists of a complete medium consisting of sugar and rice bran and sugar and oil cake. Further, as the sugar, for example, glucose, lactose, sucrose and the like are used. To this, for example, calcium chloride, which is a divalent to trivalent metal ion, is added, and further ammonium sulfate or the like is also added as appropriate. Also, as the composition ratio, for example,
Yeast extract 1%, lactose 5%, ammonium sulfate 1%, calcium chloride
There is a combination of 0.1% and the rest is water. Ammonium sulfate may be omitted if necessary.

Then, 1% of the above yeast extract and 5 lactose
%, Ammonium sulphate 1%, calcium chloride 0.1%, the rest is water.
When left overnight (about 16 hours), calcium chloride replaces sodium alginate inside the beads with calcium alginate, and gels inside the beads. During this period, the number of soil microorganisms per 1 g of beads has increased to 10 ¹⁰ to 10 ¹¹ and the number of soil microorganisms contained in the beads has increased several thousand to several tens of thousands of times.

[Second Example] When the soil microorganisms retained in the gelled bead-like interior produced by the production method of the first embodiment are left in the growth medium, a part of the soil microorganisms in the beads is gelled. The soil microorganisms survive in the growth medium by passing through the converted semipermeable membrane into the growth medium. And
The soil microorganisms are grown in the growth medium, increasing the concentration of soil microorganisms in the growth medium.

[Relationship Between Growth Medium and Soil Microbeads] The concentration of soil microorganisms in the growth medium depends on the components of the growth medium, the amount of soil microorganisms beads left in the growth medium, and the like. Like The growth medium used was yeast extract and glucose. In addition, the number of soil microorganisms after 6 days of growth was measured. From the above results, it is recognized that there is no great difference in the growth number of soil microorganisms when the amount of beads is 40% (200 g for 500 ml of medium) to 5% (25 g for 500 ml of medium). In addition, it is recognized that there is no great difference in the growth number of soil microorganisms when the glucose amount is in the range of 12% to 3%.

[Composition of Medium for Proliferation] When lactose and rice bran are used in place of glucose and yeast extract which constitute the medium for proliferation, the following is performed.
If rice bran is powdered and used as it is, it may contaminate the growth tank. Therefore, after soaking 200 g of rice bran in 1 liter of water solution for one night, the rice bran extract is used as a growth medium. did. In addition, 400 g of beads was placed in 1 liter of a growth medium, and the number of soil microorganisms after 24 hours was counted. Here, the rice bran extract (ml) and the lactose (g) are contained in 1 liter of the growth medium, and the rest is water.

The growth medium in which the soil microorganisms are alive is used as a raw material in the case of producing the gelled beads having the soil microorganisms retained therein according to the first embodiment, and contains the soil microorganisms. It is used as a deodorizing solution. In this case, by repeating the multiplication, the work of extracting the soil microorganisms from the humus can be eliminated, and the soil microorganisms can be industrially produced.

[Efficacy test] The beads in which the soil microorganisms produced in the first example were retained, and the liquid of the growth medium in which the soil microorganisms produced in the second example survived were used as deodorants. The deodorizing effect at that time was investigated. In addition, the production conditions of the beads in which the soil microorganisms of the first example are retained inside are as follows: yeast extract 1%, lactose 5%, ammonium sulfate 1%, calcium chloride 0.1%, the rest is water. The beads on which soil microorganisms were retained were left to stand overnight (about 16 hours) to grow and gel inside. In addition, the production conditions of the liquid of the growth medium in which the soil microorganisms of the second embodiment survive are as follows:
%, Glucose 6%, soil microbeads 1
00g was grown for 6 days.

(1) Ammonia The deodorizing effect on ammonia is shown in FIG. Here, a is the case where 40 g of the beads of the first embodiment were put in an odor bag and then 3 liters of nitrogen-based ammonia gas (1000 ppm) was enclosed. In b, 50 ml of the liquid of the growth medium of the second embodiment is adsorbed on absorbent cotton, put into an odor bag, and then 3 liters of nitrogen-based ammonia gas (1000 ppm) is enclosed. c is a case where 3 liters of nitrogen-based ammonia gas (1000 ppm) is enclosed in an odor bag containing nothing. From FIG. 1, it is recognized that the deodorizing effect on ammonia is considerably high.

(2) Trimethylamine The deodorizing effect on trimethylamine is shown in FIG. Here, a is the case where 40 g of the beads of the first embodiment were put in an odor bag and then 3 liters of trimethylamine gas (1000 ppm) was enclosed. b is a case where 50 ml of the liquid of the growth medium of the second embodiment is adsorbed on absorbent cotton, put into an odor bag, and then 3 liters of trimethylamine gas (1000 ppm) is enclosed. c is a case where 3 liters of trimethylamine gas (1000 ppm) is enclosed in an odor bag containing nothing. From FIG. 2, it is recognized that the deodorizing effect on trimethylamine is considerably high.

(3) Pig-house odor removal test A liquid of 1 liter of the growth medium of the second embodiment was placed in a 1 ton water tank to prepare a 1000-fold solution, which was sprayed on the whole pig-house at 2-hour intervals with a shower spray provided in the pig-house. Sprayed for 2 days.
The day after the start of spraying, the odor dropped dramatically.

(4) Sucking-up type toilet deodorizing test 20 ml of the growth medium liquid of the second example was dissolved in 1 liter of water, and sprayed evenly in the toilet tank using rain and dew. After about 1 hour, the odor disappeared. After that, the effect continues for 30 days.

(5) Deodorizing test for fisheries processing plant The liquid of the growth medium of the second example was diluted with 1000 times and 500 times and tested. The test location was as follows. Class C raw fish room ──── -5 degree C refrigerator about 300m wide
² . Remains can ──── Iron box (1m x 1m x 1m). Remains are all except fish meat (internal, bone, head, etc.). Rotary screen ──── Wastewater treatment first-stage waste collection device. Opened in two ways in a hut style. Screw press section ──── Protein recovery device for wastewater. The fish protein mud of about 80 to 90 degrees C is constantly supplied to the tank of the same size as the residual can by the belt conveyor. The odor evaluation criteria are as follows. "5" --- It smells strongly. “4” ──── It smells a little strongly. "3" --- It smells normally. "2" --- It smells faint. "1" --- Doesn't smell. "? I don't know if it smells or not.

[0036] Here, spraying was performed at 10 am on July 11th. Also,
The entire surface was sprayed, and the amount used was the amount necessary for spraying the entire surface.

[0037] Here, spraying was performed at 10 am on July 16th. Also,
The entire surface was sprayed, and the amount used was the amount necessary for spraying the entire surface.

The present invention is not limited to the above embodiments, and it goes without saying that various modifications can be made without departing from the spirit of the invention.

[0039]

As is apparent from the above description, according to the method for growing soil microorganisms according to the invention of claim 1, the number of soil microorganisms retained inside the gelled bead is several thousand to several ten thousand times. When used as a deodorant, the beaded soil microorganisms that have proliferated in this way are deodorized because the number of soil microorganisms is thousands to tens of thousands of times greater than that of non-proliferated soil microorganisms. The effect can be enhanced, the period of use of the deodorant can be extended, and the required deodorant effect can be obtained with a small amount of the deodorant used.

According to the method for growing soil microorganisms according to the second aspect of the present invention, since the soil microorganisms propagate in the medium for growing the soil microorganisms retained inside the gelled bead-shaped interior, the proliferation of the soil microorganisms occurs. It is possible to propagate the soil microorganisms retained inside the gelled bead using the soil microorganisms propagated in the culture medium, so only the soil microorganisms are first extracted from the humus, and then this is repeated. Thus, it is possible to produce soil microorganisms, which are the raw material of the deodorant, inexhaustibly, and there is an extremely new and beneficial effect.

[Brief description of drawings]

FIG. 1 is a diagram showing a deodorizing effect on ammonia.

FIG. 2 is a diagram showing the deodorizing effect on trimethylamine.

Claims (2)

[Claims] 1. A mixture of water to a humus containing soil microorganisms, which is then filtered to obtain an aqueous solution of soil microorganisms, the pH of which is adjusted by adding an alkaline aqueous solution to precipitate iron ions and the like in the soil microorganism extract. And filtered to remove the precipitate,
An aqueous solution of sodium alginate is added to this and mixed with stirring to prepare a soil microbial extract in the sol state, and the soil microbial extract in the sol state is dropped from the pores into an aqueous solution containing divalent to trivalent metal ions. Soil microorganisms are retained inside the beaded surface gelled, and the soil microorganisms retained inside the beaded surface gelled are left to stand in a growth medium containing 2-3 valent metal ions, A method for growing soil microorganisms, which comprises increasing the concentration of soil microorganisms retained inside a bead-like gel that has gelled to the inside. 2. The gelled bead-shaped soil microorganisms of claim 1 are allowed to stand in a growth medium to release the soil microorganisms in the beads into the growth medium, and the medium is grown in the growth medium. A method for growing soil microorganisms, which comprises increasing the concentration of soil microorganisms.