JPH0717499B2 - Caries fungus growth inhibitor - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION <Industrial Application Field> The present invention relates to Strepto-coccus mutan, which is greatly involved in the formation of caries (caries).
The present invention relates to a cariogenic growth inhibitor which has excellent antibacterial activity against oral bacteria represented by s) and can prevent glycan formation involving oral microorganisms.

<Prior art> Conventionally, as agents that inhibit the growth of oral bacteria, chlorhexidine, fungicides such as alexidine and penicillin,
The use of antibiotics such as tetracycline is known, and many synthetic and natural products have been reported.

<Problems to be Solved by the Invention> However, these known growth inhibitory agents for oral bacteria have a safety problem for human body, a stability problem, and a stability depending on the administration method and dose. Is confirmed to have low antibacterial properties, or when administered,
There were drawbacks such as an unpleasant odor.

<Object of the Invention> The object of the present invention is very effective in inhibiting the growth of Streptococcus mutans, which is a dental caries, and is also pleasing even in suppressing dextran (glucan) formation that causes plaque. It is intended to provide a caries fungus growth inhibitor having an aroma.

<Means for Solving Problems> According to the present invention, trans-pinocarbeol, para-
There is provided a caries growth inhibitor containing at least one selected from the group consisting of isopropylcyclohexanol, campholenic aldehyde, γ-decalactone and γ-undecalactone as an active ingredient.

The present invention will be described in more detail below.

Generally, the cause of caries formation is Teeth
h), substrate (Substrate) and mutans (St.mutan)
s), and when these three factors are present at the same time, it is said that caries occur. The mechanism of caries formation is that when sugar is used as a substrate (Substrate) among sugars, this sugar is insoluble in water due to glucosyltransferase (enzyme) produced by St. mutans. It changes into sexual dextran (glucan) and forms plaque on the surface of teeth (Teeth). It is said that the dental plaque becomes anaerobic and fermentation progresses due to lactic acid bacteria to produce lactic acid, which lowers the pH, and this lactic acid decalcifies tooth enamel to form caries (caries).

Therefore, Streptococcus mutans (St.mutan
Suppressing the growth of oral bacteria represented by s) and inhibiting the formation of dental plaque is an effective method for preventing the formation of caries.

The present inventors have searched for a substance that inhibits the growth of Streptococcus mutans, which is a caries fungus that is largely involved in the formation of dextran (glucan) that causes dental plaque, and results in trans-pinocarbeol and para. It has been found that one or more compounds selected from the group consisting of: isopropylcyclohexanol, camphorenic aldehyde, γ-decalactone and γ-undecalactone are very effective. These compounds are known perfume substances that have already been confirmed to be safe for the human body, but such perfume substances that have a pleasant aroma in the oral cavity can effectively inhibit the growth of Streptococcus mutans. This has never been known.

As the administration form of the caries growth inhibitor of the present invention, its effect can be expected by adding it to tablet confectionery, chewing gum, candy, troche, etc., which has a relatively long residence time in the oral cavity, and antibacterial activity by a surfactant. Since there is almost no decrease in the amount, it can be used even when blended with an oral cleaning agent such as toothpaste or mouthwash. The blending amount is not necessarily uniform depending on the form, kind, etc. of the target product, but it is generally 0.005 to 10% by weight, preferably 0.05 to 5% by weight in a commonly used oral composition. Effectively develops a caries prevention effect.

<Effect of the Invention> The caries bacterium growth inhibitor of the present invention has a pleasant fragrance, and since it effectively inhibits the growth of caries bacterium, its utility value is high.

<Example> Hereinafter, the antibacterial activity effect of the present invention will be described in detail with reference to Examples.

Example 1 A 5% -alcohol solution of a sample compound was added to a liquid medium of a heart infusion broth containing 2% of sucrose so that the concentration of the sample compound was 0.1% (1/1000 concentration),
The pre-cultured Streptococcus mutans 3125001 strain was inoculated with a platinum loop and cultured at 37 ° C. for 24 hours. Next, the culture solution was stirred to disperse the precipitate, and then the absorbance (turbidity) at 550 nm was measured to check the amount of glucan formed by mutans bacteria. The pH of the culture solution was also measured because sugar fermentation by mutans bacteria occurs during glucan formation and the medium becomes acidic.

The results are shown in Table 1.

The amount of plaque formation was expressed as a percentage when the control (sterile water) was 100%.

As shown in Table 1, no decrease in pH of the medium and glucan formation were observed, which is clearly effective in inhibiting the growth of mutans.

(Example-2) With respect to the compounds of Table-1 in which the decrease of the pH of the medium and the formation of glucan were not observed at all in Example-1, the sample compound was diluted with the same medium and the same method as in Example-1. Then, an antibacterial test was conducted to determine the minimum effective inhibitory concentration of each compound. The evaluation of the lowest effective inhibitory concentration test was carried out by the change in the pH of the medium and the amount of glucan formed, as in Example-1. As a result of the judgment, those in which the pH of the medium was not lowered and glucan formation was not observed at all were considered to have antibacterial activity, and the reciprocal of the lowest effective concentration of the test sample was used as the anti-mutans activity value. The results are shown in Table-2.

As is clear from Table 2, all the compounds of the present invention show high activity.

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Claims (1)

[Claims] 1. An active ingredient containing at least one compound selected from the group consisting of trans-pinocarbeol, para-isopropylcyclohexanol, camphorenic aldehyde, γ-decalactone and γ-undecalactone. A characteristic inhibitor of caries growth.