JPH0722495B2 - Chlorella-containing oil and fat food - Google Patents
Chlorella-containing oil and fat foodInfo
- Publication number
- JPH0722495B2 JPH0722495B2 JP2017699A JP1769990A JPH0722495B2 JP H0722495 B2 JPH0722495 B2 JP H0722495B2 JP 2017699 A JP2017699 A JP 2017699A JP 1769990 A JP1769990 A JP 1769990A JP H0722495 B2 JPH0722495 B2 JP H0722495B2
- Authority
- JP
- Japan
- Prior art keywords
- oil
- chlorella
- extract
- fat
- raw material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/60—Edible seaweed
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS OR COOKING OILS
- A23D9/00—Other edible oils or fats, e.g. shortenings or cooking oils
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/115—Fatty acids or derivatives thereof; Fats or oils
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/15—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Nutrition Science (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Zoology (AREA)
- Marine Sciences & Fisheries (AREA)
- Seeds, Soups, And Other Foods (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
- General Preparation And Processing Of Foods (AREA)
- Preparation Of Fruits And Vegetables (AREA)
- Grain Derivatives (AREA)
- Edible Oils And Fats (AREA)
- Jellies, Jams, And Syrups (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
【発明の詳細な説明】 〔産業上の利用分野〕 この発明は、クロレラ藻体、菌茸類および強壮薬用植物
の各種有効成分を有効且つ安定的に保持する機能性健康
食品として有用なクロレラ含有油脂食品およびその製造
方法に関するものである。DETAILED DESCRIPTION OF THE INVENTION [Industrial field of application] The present invention contains chlorella useful as a functional health food that effectively and stably retains various active ingredients of chlorella algal cells, fungi and tonic medicinal plants. The present invention relates to an oil and fat food and a method for producing the same.
クロレラ藻体は淡水産の単細胞藻類の一種であり、蛋白
質などの含有量が多く、さらに、特有の各種生理活性作
用、例えば動物または微生物の発育促進作用、食品類の
調味作用、食品類の異臭を除去する作用などを有してい
ることが知られており、とりわけクロレラ藻体の熱水抽
出液は上記発育促進作用、調味作用などを顕著に有する
と共に濃縮液体形態の製品化が可能であるためその応用
範囲が広汎に及ぶに至つている。Chlorella alga is a kind of freshwater unicellular algae, has a high content of proteins and the like, and further has various peculiar bioactive actions, such as growth promoting action of animals or microorganisms, seasoning action of foods, and offensive odor of foods. It is known that it has an action of removing chlorophyll and the like, and in particular, a hot water extract of Chlorella algal cells has the above-mentioned growth promoting action, seasoning action and the like, and can be commercialized in a concentrated liquid form. Therefore, its application range has reached widespread.
このようなクロレラ藻体の有用性に着目して、従来、種
々のクロレラ藻体利用製品が開発されるに至つている
が、その代表的なものとしては、例えばクロレラ藻体の
乾燥粉末をニンニク粉末などと混和し錠剤に成型したも
の、クロレラ熱水抽出液を麺類、水産練製品などの製造
時において呈味成分、製品改良成分などとして添加使用
したもの、クロレラ藻体の乾燥粉末または熱水抽出液を
他の栄養成分、薬効成分などと共にゼラチンカプセルな
どに充填し健康食品としたもの、など多種多様であり、
その応用範囲はかなり広汎に及んでいる状況にある。Focusing on the usefulness of such Chlorella algal cells, various products utilizing Chlorella algal cells have been developed so far, and a typical example thereof is a dry powder of Chlorella algal cells such as garlic. Mixture with powder etc. and molded into tablets, chlorella hot water extract added and used as taste component, product improving component, etc. at the time of manufacturing noodles, fish paste products, dry powder of chlorella algae or hot water There are a wide variety of things such as filling capsules with the extract along with other nutritional ingredients, medicinal ingredients, etc. as health foods,
The range of application is quite wide.
このように、クロレラ藻体の有用性に着目し、従来、各
種のクロレラ藻体利用製品が開発されているが、これま
でのクロレラ藻体利用製品は、多くの場合、その製造過
程における処理操作に問題点があり、クロレラ藻体に含
有される有効成分が必ずしも有効且つ安定的に保持され
ていないという欠点があつた。すなわち、従来、学術的
試験研究結果としてクロレラ藻体の各種有用性が確認さ
れ報告されているにもかかわらず、これを実際に実用商
品として製品化した場合、その有効成分を有効且つ安定
的に保持させることが困難であり、その有効成分を確実
に有効利用し得ないという問題点があつた。特に、クロ
レラ藻体を他の薬効成分を含有する薬用原料などと併用
し各有効成分を相剰効果的に活用した健康食品を製造す
る場合、各原料の薬効成分などを有効且つ安定的に保持
せしめて製品化を図ることが製品開発上最も重要な事柄
であるが、前述した如く従来のクロレラ製品は多くの場
合、薬効成分などが必ずしも有効且つ安定的に保持され
ておらずその有用性を充分に活性し得ないという問題点
があつた。Thus, while focusing on the usefulness of chlorella algal cells, various products utilizing chlorella algal cells have been developed in the past, but the products utilizing chlorella algal cells to date are often treated in the manufacturing process. However, the active ingredient contained in the Chlorella algal cells is not always effectively and stably retained. That is, conventionally, although various usefulness of Chlorella algal cells have been confirmed and reported as academic test results, when actually commercialized as a commercial product, its active ingredient is effectively and stably produced. There is a problem that it is difficult to retain the active ingredient and the active ingredient cannot be effectively utilized. In particular, when producing health foods in which Chlorella algal cells are used in combination with other medicinal ingredients that contain other medicinal ingredients to effectively utilize each active ingredient, the medicinal ingredients of each ingredient are effectively and stably retained. At the very least, commercialization is the most important thing in product development, but as described above, in many cases, conventional chlorella products do not always have effective and stable retention of medicinal components and their usefulness. There was a problem that it could not be activated sufficiently.
そこで、本発明者は、クロレラ藻体を用いた機能性健康
食品を製品化するに際して、クロレラ藻体およびその他
の薬用原料などの有効成分をその活性を低減させること
なく有効且つ安定的に保持せしめた製品を開発すること
を目標として鋭意研究を積み重ねた結果、クロレラ藻体
の熱水抽出液の濃縮エキスと特に菌茸類の熱水抽出液の
濃縮エキスとを包接材を加えて包接練合し乾燥して得ら
れる混合エキス末原料、強壮薬用植物およびクロレラ藻
体をビタミンE油の存在下に擦合して得られるスラリー
状原料、を用いること、さらにこれらを必要によりレシ
チンなどを添加した食用油脂に加えて均質に撹拌し分散
混和し、軟質カプセルに封入することにより所期の目的
を達成し得ることを見出し、この発明を完成するに至つ
た。すなわち、この発明は、クロレラ藻体、菌茸類およ
び強壮薬用植物などの有効成分を有効且つ安定的に保持
した機能性クロレラ含有油脂食品の製造方法を提供する
ことを目的とするものである。Therefore, the present inventor, when commercializing a functional health food using chlorella algal cells, keep active ingredients such as chlorella algal cells and other medicinal raw materials effectively and stably without reducing their activity. As a result of intensive research aimed at developing a new product, the inclusion of the concentrated extract of the hot water extract of Chlorella algae and especially the concentrated extract of the hot water extract of fungi with fungi Using a mixed extract powder raw material obtained by kneading and drying, a slurry-like raw material obtained by rubbing a tonic medicinal plant and a chlorella algal body in the presence of vitamin E oil, and further adding lecithin and the like as necessary. The inventors have found that the desired object can be achieved by adding the added edible oil and fat, stirring the mixture homogeneously, dispersing and mixing, and encapsulating in a soft capsule, and have completed the present invention. That is, an object of the present invention is to provide a method for producing a functional chlorella-containing oil and fat food product in which active ingredients such as chlorella algal cells, fungi and tonic plants are effectively and stably retained.
また、この発明は、クロレラ藻体の各種生理活性成分を
多量に含有すると共に、菌茸類および強壮薬用植物など
の有効成分をも有効且つ安定的に含有する機能性を有す
る健康食品として有用なクロレラ含有油脂食品を提供す
ることを目的とするものである。Further, the present invention contains a large amount of various physiologically active components of Chlorella algal cells, and is useful as a health food having functionality that effectively and stably contains active components such as fungi and tonic plants. It is intended to provide a chlorella-containing oil and fat food.
さらに、この発明は、食用油脂を含有し、直接料理用調
味素材などとしても利用することが可能な機能性クロレ
ラ含有油脂食品を提供することを目的とするものであ
る。A further object of the present invention is to provide a functional chlorella-containing oil and fat food that contains edible oil and fat and can be directly used as a seasoning material for cooking.
そして、このような目的を達成するために採用したこの
発明の構成は、次の〜の技術的手段から成るもので
ある。The configuration of the present invention adopted to achieve such an object comprises the following technical means.
クロレラ藻体の熱水抽出液の濃縮エキスと菌茸類の
熱水抽出液の濃縮エキスとを包接材を加え包接混合し乾
燥して得られる混合エキス末原料と、強壮薬用植物およ
びクロレラ藻体をビタミンE油の存在下に擦合して得ら
れるスラリー状原料とを、必要によりレシチンなどを添
加した食用樹脂と均質に撹拌混合することにより製造さ
れる油性懸濁液を軟質カプセルに充填し封入することを
特徴とするクロレラ含有油脂食品の製造方法。Chlorella A mixed extract powder raw material obtained by adding a clathrate to a concentrated extract of hot water extract of algae and a concentrated extract of hot water extract of fungi and mixing and drying, and a tonic herb and chlorella An oily suspension produced by homogeneously stirring and mixing a slurry-like raw material obtained by rubbing algal cells in the presence of vitamin E oil with an edible resin to which lecithin or the like has been added is soft capsules. A method for producing a chlorella-containing oil and fat food, which comprises filling and enclosing.
包接材として、サイクロデキストリンを使用する上
記第項記載のクロレラ含有油脂食品の製造方法。The method for producing a chlorella-containing fat or oil food according to the above item, wherein cyclodextrin is used as an inclusion material.
菌茸類として、カワラタケ、サルノコシカケ、シイ
タケのうちの少なくとも一種を使用する上記第項記載
のクロレラ含有油脂食品の製造方法。The method for producing a chlorella-containing oil and fat food according to the above item, wherein at least one kind of Pleurotus cornucopiae, Pleurotus cornucopiae, and Shiitake mushroom is used as the fungi.
強壮薬用植物として、ニンニク、朝鮮人参、アロ
エ、梅、ウコギ、ハトムギのうちの少なくとも一種を使
用する上記第項記載のクロレラ含有油脂食品の製造方
法。The method for producing a chlorella-containing oil and fat food according to the above item, wherein at least one of garlic, ginseng, aloe, plum, pearl barley, and adlay is used as a tonic drug.
練合を加温減圧下で行う上記第項記載のクロレラ
含有油脂食品の製造方法。The method for producing a chlorella-containing oil and fat food according to the above item, wherein kneading is performed under heating and reduced pressure.
食用油脂類として、落花生油、ゴマ油、小麦胚芽
油、菜種油、大豆油、ヤシ油、サフラワー油、ミツロウ
のうちの少なくとも一種を使用する上記第項記載のク
ロレラ含有油脂食品の製造方法。The method for producing a chlorella-containing oil and fat food according to the above item, wherein at least one of peanut oil, sesame oil, wheat germ oil, rapeseed oil, soybean oil, coconut oil, safflower oil, and beeswax is used as the edible oil and fat.
クロレラ藻体の熱水抽出液の濃縮エキスと菌茸類の
熱水抽出液の濃縮エキスとを包接材を加え包接練合し乾
燥して得られる混合エキス末原料と、強壮薬用植物およ
びクロレラ藻体をビタミンE油の存在下に擦合して得ら
れるスラリー状原料とを、必要によりレシチンなどを添
加した食用油脂と均質に撹拌混合することにより製造さ
れる油性懸濁液を軟質カプセルに充填封入することによ
り製造されることを特徴とするクロレラ含有油脂食品。A mixed extract powder raw material obtained by adding a clathrate to the concentrated extract of the hot water extract of Chlorella algae and the concentrated extract of the hot water extract of fungi and kneading and drying, and a tonic herb and Soft capsules of an oily suspension produced by homogeneously stirring and mixing a slurry-like raw material obtained by rubbing Chlorella algal cells in the presence of vitamin E oil with edible oil and fat to which lecithin or the like is added if necessary. A chlorella-containing oil / fat food product characterized by being produced by filling and encapsulating the same.
この発明において、クロレラ藻体の熱水抽出液の濃縮エ
キス、菌茸類の熱水抽出液の濃縮エキス、強壮薬用植物
のエキスなどの原料に対して比較的多量の食用油脂が使
用されるが、これは、目的製品の製造工程中および製品
の保存過程において、軟質カプセル剤が上記クロレラ藻
体、菌茸類および強壮薬用植物などの有効成分含有エキ
ス中に存在する水分によつて膨潤、溶解などの変質を生
起することを防止すると共に、製品を料理用、特にいた
め料理用調味素材などとして多角的に利用できるように
するためである。また、食用油脂中に上記有効成分含有
エキスを均質に撹拌混合するのも軟質カプセル剤の膨潤
破壊などを防止するためである。In this invention, a relatively large amount of edible oil and fat is used as a raw material such as a concentrated extract of a hot water extract of Chlorella algal cells, a concentrated extract of a hot water extract of fungi, and an extract of a tonic drug. , This is because during the manufacturing process of the target product and during the storage process of the product, the soft capsule swells and dissolves due to the water present in the active ingredient-containing extract of the above chlorella algal cells, fungi and tonic herbs. This is to prevent the occurrence of such alterations and to allow the product to be used in various ways as a seasoning material for cooking, especially for cooking. Further, the reason for uniformly stirring and mixing the above-mentioned active ingredient-containing extract in edible fats and oils is to prevent swelling and destruction of the soft capsule.
この発明におけるクロレラ藻体の熱水抽出液とは、光合
成による屋外大量培養により生産されるクロレラ正藻体
の懸濁液を約80〜100℃の温度で15〜20分間程度加熱処
理した後、クロレラ藻体を分別して得られる抽出エキス
である。クロレラ生藻体としては、クロレラ・ピレノイ
ドーサ、クロレラ・ブルガリス・クロレラ・エリプソデ
アセネデスムスなどの淡水産の単細胞藻類が代表的なも
のとしてあげられるが特に限定されるものではない。ク
ロレラ藻体の熱水抽出液は、蛋白質、ビタミン類などを
はじめ、クロレラ藻体の細胞壁に存在するN・β−1・
3グルカン、酸性ラムナンなどの有効多糖類など、各種
生理活性物質を含有する。また、クロレラ藻体の熱水抽
出液は、光合成クロレラ藻体粉末に、当該藻体粉末の約
4倍容量以上の水を添加し混合して、50〜120℃の温度
で約1時間以上加熱することにより、ゆっくりと抽出処
理して得られる抽出エキスであつてもよく、適宜の熱水
抽出液が使用される。また、菌茸類の熱水抽出液は、菌
茸類菌体、乾燥菌茸類菌体、菌茸類菌体粉末などの熱水
抽出エキスであり、例えば、菌茸類菌体粉末に、当該菌
体粉末の約4倍容量以上の水を添加し混合して、約45〜
120℃の温度で長時間、好ましくは5〜7時間緩慢に加
熱処理して得られる抽出エキスなど、適宜の熱水抽出液
が使用される。菌茸類としては、サルノコシカケ、カワ
ラタケ、シイタケ、その他の食用キノコ類が適宜使用さ
れる。The hot water extract of Chlorella alga in this invention is a suspension of Chlorella normal alga produced by outdoor mass culture by photosynthesis after heat treatment at a temperature of about 80 to 100 ° C for about 15 to 20 minutes, It is an extract obtained by separating Chlorella algae. Examples of the chlorella alga are representative of freshwater unicellular algae such as chlorella pyrenoidosa and chlorella bulgaris chlorella ellipso de acenedesmus, but are not particularly limited. The hot water extract of Chlorella algal cells contains proteins, vitamins, etc., and N · β-1 · present in the cell wall of Chlorella algal cells.
3 Contains various physiologically active substances such as effective polysaccharides such as glucan and acidic rhamnan. The hot water extract of Chlorella algal cells is prepared by adding approximately 4 times or more the volume of water of the algal cell powders to the photosynthetic Chlorella algal powder and mixing them, and heating at a temperature of 50 to 120 ° C for about 1 hour or more. By doing so, the extract may be an extract obtained by a slow extraction treatment, and an appropriate hot water extract is used. The hot water extract of fungi is a hot water extract such as fungi fungus bodies, dried fungus fungus fungus bodies, fungi fungus fungus fungus body powders, for example, fungi fungus fungus fungus body powders, Add about 4 times more water than the bacterial cell powder and mix,
An appropriate hot water extract, such as an extract obtained by slowly heating at 120 ° C. for a long time, preferably 5 to 7 hours, is used. As mushrooms, edible mushrooms, edible mushrooms, shiitake mushrooms and other edible mushrooms are appropriately used.
強壮薬用植物としては、滋養強壮の効のある薬用植物で
あれば如何なるものでもよいが、特にニンニク、朝鮮人
参、アロエ、梅、ウコギ、ハトムギなどが好適に使用さ
れる。強壮薬用植物は、植物体、エキスまたは乾燥粉末
など適宜の形態のものが使用される。なお、ここでエキ
スとは、強壮薬用植物を常法により浸出して得た浸出
液、その濃縮液、植物体をコロイドミルなどで擦合して
得たスラリー状エキスなどであり、また、乾燥粉末とし
ては、植物体を適宜前処理した後、熱風乾燥、凍結乾
燥、噴霧乾燥など適宜の乾燥手段により乾燥処理して得
られる乾燥粉末あるいは前記植物体のエキスを常法によ
り乾燥処理して得られる乾燥粉末などが使用される。As the tonic medicinal plant, any medicinal plant having a nutritional tonic effect may be used, but garlic, ginseng, aloe, plum, pearl barley, adlay and the like are preferably used. As the tonic plant, a plant, an extract or a dry powder having an appropriate form is used. The extract here is a leachate obtained by leaching a tonic herb by a conventional method, a concentrate thereof, a slurry extract obtained by rubbing a plant body with a colloid mill, or the like, and a dry powder. As, after the plant is appropriately pretreated, dried by a suitable drying means such as hot air drying, freeze-drying, spray drying, or a dried powder obtained by drying the plant extract by a conventional method. Dry powder or the like is used.
前記クロレラ藻体の熱水抽出液、菌茸類の熱水抽出液
は、それぞれ濃縮機により濃縮しスラリー状の濃縮エキ
スとした後、包接化合物を形成する包接材と共に練合機
にて包接練合することによりこれらのエキスの包接練合
物を得る。当該包接練合物は、乾燥機にて規定の水分量
まで乾燥した後、粉砕機にて規定のメツシユに粉砕した
エキス粉末の形態で使用することが望ましい。The hot water extract of Chlorella algae, the hot water extract of fungi, is a concentrated extract in the form of a slurry concentrated by a concentrator, respectively, in a kneader with an inclusion material forming an inclusion compound. The inclusion clathrates of these extracts are obtained by the clathrate kneading. The clathrate product is preferably dried in a dryer to a specified water content and then used in the form of an extract powder crushed into a specified mesh by a crusher.
強壮薬用植物は、各植物体あるいはそのエキス粉末など
をクロレラ藻体と共にビタミンE油の存在下にコロイド
ミルにて擦合してスラリー状のエキスとしたものを原料
として使用する。The tonic plant is prepared by rubbing each plant or its extract powder with a Chlorella algae in the presence of vitamin E oil in a colloid mill to form a slurry extract as a raw material.
食用油脂としては、落花生油、ゴマ油、小麦胚芽油、菜
種油、大豆油、ヤシ油、サフラワー油、ミツロウその他
食用に供され得る油脂類が単独あるいは混合して使用さ
れる。食用油脂を混合使用する場合は、原料食用油脂を
加熱混合し、さらに必要に応じて加熱溶解させた大豆レ
シチンなどを添加し、ホモミキサーにて混合撹拌後、室
温にて冷却した油性乳化原料の形で使用することが望ま
しい。As the edible oil and fat, peanut oil, sesame oil, wheat germ oil, rapeseed oil, soybean oil, coconut oil, safflower oil, beeswax, and other edible oils and fats may be used alone or in combination. When using mixed edible fats and oils, the raw material edible fats and oils are heated and mixed, and soybean lecithin and the like that are heated and dissolved as necessary are further added, and after mixing and stirring with a homomixer, the oily emulsified raw material cooled at room temperature It is desirable to use it in the form.
食用油脂に、クロレラ藻体、菌茸類、強壮薬用植物など
の有効成分含有原料を食用油脂と均質に混合するには、
前記包接練合物の混合エキス粉末原料と、強壮薬用植物
およびクロレラ藻体をビタミンE油の存在下に擦合して
得られるスラリー状原料とを、前記食用油脂と混合機に
て撹拌混合して均質化すればよい。また、各原料の混合
手段、混合順序などについては特に限定されるものでは
ないが、一般的には、強壮薬用植物およびクロレラ藻体
をビタミンE油の存在下に擦合して得られるスラリー状
原料を油性乳化原料の食用油脂と均質に撹拌混和した
後、この油性懸濁液に包接練合物の混合エキス粉末原料
を均質に混合することが望ましい。また、強壮薬用植物
などの有効成分含有エキス、包接原料練合物の乾燥粉末
原料、食用油脂原料とを同時的に均質に混合することも
適宜採用される。To mix edible oils and fats with active ingredient-containing raw materials such as chlorella algae, fungi, tonic plants, etc., to mix them uniformly,
The mixed extract powder raw material of the inclusion clathrate and a slurry-like raw material obtained by rubbing a tonic drug plant and a chlorella algal body in the presence of vitamin E oil are mixed with the edible oil and fat by stirring with a mixer. And homogenize. Further, the mixing means, mixing order, etc. of the respective raw materials are not particularly limited, but in general, a slurry obtained by rubbing a tonic plant and chlorella algal cells in the presence of vitamin E oil It is desirable that the raw material is homogeneously stirred and mixed with the edible oil and fat as the oily emulsified raw material, and then the mixed extract powder raw material of the clathrate mixture is uniformly mixed with this oily suspension. Further, it is also appropriately adopted to simultaneously simultaneously and uniformly mix an extract containing an active ingredient such as a tonic plant, a dry powder raw material of a clathrate raw material kneading, and an edible oil / fat raw material.
強壮薬用植物およびクロレラ藻体を擦合する際に、ビタ
ミンE油を使用することにより、ビタンミンEの生理活
性、および強壮薬用植物などの各種有効成分の生理活性
を有効且つ安定的に強化、保持させることができる。By using Vitamin E oil when rubbing tonic herbs and Chlorella algal bodies, the physiological activity of Vitamin E and various active ingredients such as tonic plants are effectively and stably enhanced and retained. Can be made.
続いて、このように各種原料を混合して得た油性懸濁液
を規定メツシユの篩を通過させ、脱気装置にて脱泡した
後、ソフトゼラチンカプセルなど適宜の軟質カプセルに
充填し封入することにより目的の機能性クロレラ含有油
脂食品が得られる。なお、強壮薬用植物原料として植物
体浸出液を使用し、これを噴霧乾燥体などとして使用す
ることもできるが、水分があまりにも多く高価につくの
で、他の強壮薬用植物の乾燥粉末などを添加し、または
添加することなく、ラクトース、澱粉などの可食性糖質
粉末等に吸着させた粉末体として使用することが望まし
い。Subsequently, the oily suspension obtained by mixing the various raw materials in this way is passed through a sieve of a prescribed mesh and degassed by a deaerator, and then filled and sealed in an appropriate soft capsule such as a soft gelatin capsule. As a result, the target functional chlorella-containing oil / fat food product is obtained. It is possible to use a plant exudate as a tonic plant material and use it as a spray-dried product, but since it contains too much water and is expensive, dry powder of other tonic plants should be added. Or, it is desirable to use it as a powder body adsorbed on edible sugar powder such as lactose or starch without adding it.
また、これらのラクトース、澱粉などの可食性糖質粉末
は、従来知られているような単に増量剤、賦型剤として
使用するためではなくして、前記浸出液の水分を吸収さ
せて、軟質カプセルに悪影響を与えないようにするため
に好適に使用されるものである。Further, these edible sugar powders such as lactose and starch are not merely used as conventionally known bulking agents and excipients, but are allowed to absorb the water of the exudate to form soft capsules. It is preferably used to prevent adverse effects.
なお、クロレラ藻体、菌茸類強壮薬用植物の各有効成分
含有エキス原料等の食用油脂原料に対する配合割合は、
特に限定されるものではなく、製品の種類等に応じて適
宜選択することができる。The blending ratio of chlorella algae, edible oils and fats raw materials, such as raw materials for each active ingredient-containing extract of fungi mushroom tonic plants, is:
It is not particularly limited, and can be appropriately selected depending on the type of product.
続いて、本発明の実施例に基づいて本発明をさらに具体
的に説明する。Next, the present invention will be described more specifically based on the examples of the present invention.
実施例1. 光合成クロレラ粉末30kgに対し、水300の割合で混合
し、熱水抽出機にて有効成分を抽出し、濃縮機にて約20
倍に濃縮してスラリー状の濃縮エキスを得た。Example 1. 30 kg of photosynthetic chlorella powder was mixed with 300 parts of water, the active ingredient was extracted with a hot water extractor, and about 20 with a concentrator.
It was concentrated twice to obtain a slurry-like concentrated extract.
一方、乾燥カワラタケ25kgに対し、水400の割合で混
合し、熱水抽出液にて有効成分を抽出し、濃縮機にて約
20倍に濃縮してスラリー状の濃縮エキスを得た。On the other hand, dry Kawaratake (25 kg) is mixed with water at a ratio of 400, the active ingredient is extracted with a hot water extract, and concentrated with a concentrator.
The concentrate was concentrated 20 times to obtain a slurry-like concentrated extract.
これらの濃縮エキスに、包接材のサイクロデキストリン
9.5kgを加え、加温減圧しながら練合機にて包接練合
し、次いで乾燥機にて乾燥後、粉砕機にて粉砕して混合
エキス粉末原料14kgを得た。Cyclodextrin as a clathrate is added to these concentrated extracts.
9.5 kg was added, and inclusion and kneading was performed with a kneader while heating and depressurizing, dried with a drier, and then pulverized with a pulverizer to obtain 14 kg of a mixed extract powder raw material.
さらに、朝鮮人参乾燥粉末8.5kg、無臭ニンニクエキス
末8.5kg、ウコギ1kgに、光合成クロレラ末90kgを混合し
たものを、コロイドミルにて、天然ビタミンE油20kgと
擦り合わせスラリー状の原料第142kgを得た。Furthermore, dried ginseng powder 8.5 kg, odorless garlic extract powder 8.5 kg, ukogi 1 kg, and photosynthetic chlorella powder 90 kg were mixed with a natural vitamin E oil 20 kg in a colloid mill to produce a raw material 142 kg in a slurry state. Obtained.
このようにして得られた各原料を大豆レシチン15kgを添
加しホモミキサーにて撹拌混合した落花生油28kg、サフ
ラワー油167kgを含む油性乳化原料210kgに加え、均質に
撹拌混合し、油性懸濁液366kgを得た。次いで、この油
性懸濁液を脱気装置にて脱泡した後、ソフトゼラチンカ
プセルに充填し封入してクロレラ含有油脂食品を得た。Soybean lecithin 15 kg of each raw material thus obtained was added to 210 kg of an oily emulsified raw material containing peanut oil that was stirred and mixed with a homomixer and 167 kg of safflower oil, and uniformly mixed with stirring to obtain an oily suspension. I got 366 kg. Next, this oily suspension was defoamed with a deaerator, and then filled into a soft gelatin capsule and sealed to obtain a chlorella-containing oil and fat food.
実施例2 光合成クロレラ粉末30kgに対し、水300の割合で混合
し、熱水抽出機にて有効成分を抽出し、濃縮機にて約20
倍に濃縮してスラリー状の濃縮エキス原料を得た。Example 2 30 kg of photosynthetic chlorella powder was mixed with 300 parts of water, the active ingredient was extracted with a hot water extractor, and about 20 with a concentrator.
It was concentrated twice to obtain a concentrated extract raw material in the form of a slurry.
一方、乾燥シイタケ25kg、乾燥カワラタケ10kgに対し、
水450の割合で混合し、熱水抽出機にて有効成分を抽
出し、濃縮機にて約20倍に濃縮してスラリー状の濃縮エ
キス原料を得た。On the other hand, for 25 kg of dried shiitake and 10 kg of dried kawatake mushroom,
Water was mixed at a ratio of 450, the active ingredient was extracted with a hot water extractor, and concentrated about 20 times with a concentrator to obtain a slurry-like concentrated extract raw material.
これらの濃縮エキス原料に、包接材のサイクロデキスト
リン10.5kgを添加し、練合機にて加温減圧しながら包接
練合し、包接練合物を得た。次いで乾燥機にて乾燥後、
粉砕機にて粉砕し混合エキス粉末原料16kgを得た。10.5 kg of a cyclodextrin as a clathrate was added to these concentrated extract raw materials, and the clathrate was kneaded with a kneader while heating and depressurizing to obtain a clathrate kneaded product. Then after drying in a dryer,
The mixture was pulverized with a pulverizer to obtain 16 kg of a mixed extract powder raw material.
さらに、光合成クロレラ粉末130kg、ガーリツクエキス
9.5kg、ハトムギ乾燥粉末9.5kg、ウコギ10.5kg、を混合
したものを、コロイドミルにて、天然ビタミンE油25kg
と少量づつ擦合してスラリー状のエキス原料184.5kgを
得た。In addition, 130 kg of photosynthetic chlorella powder, garlic extract
A mixture of 9.5 kg, dried pearl barley powder 9.5 kg and ukogi 10.5 kg was mixed with a colloid mill to obtain 25 kg of natural vitamin E oil.
184.5 kg of an extract raw material in the form of a slurry was obtained by rubbing with each other little by little.
これらの各エキス原料を、粘土のある油性混合液すなわ
ち、ゴマ油28kg、落花生精油28kg、サフラワー油110kg
を加熱混合し、さらに大豆レシチン18kgを添加しホモキ
サーにて撹拌混合して得られる油性乳化原料184kgと均
質に混合した後、脱気装置にて脱泡して製造した油性懸
濁液384.5kgをソフトゼラチンカプセルに充填し封入し
て、目的のクロレラ含有油脂食品を得た。Each of these extract raw materials is an oily mixed solution with clay, that is, sesame oil 28 kg, peanut essential oil 28 kg, safflower oil 110 kg
Was mixed by heating, and 18 kg of soybean lecithin was further added and homogeneously mixed with 184 kg of an oily emulsified raw material obtained by stirring and mixing with a homoxer, and then 384.5 kg of an oily suspension produced by defoaming with a deaerator. A soft gelatin capsule was filled and encapsulated to obtain the target chlorella-containing oil and fat food.
本発明のクロレラ含有油脂食品の製造方法は、クロレラ
藻体、菌茸類、強壮薬用植物などの各種有効成分を有効
且つ安定に保持し、各有効成分の長期保存性に優れた製
品を製造することができる利点があり、さらに本発明の
クロレラ含有油脂食品は、コレステロール低下作用など
の優れた生理活性作用を有する食用油脂などを多量に含
んでおり、且つ各成分原料が均質に混和されているので
機能性健康食品としてその有用性はきわめて高い。The method for producing a chlorella-containing oil and fat food of the present invention effectively and stably holds various active ingredients such as chlorella algal cells, fungi, tonic plants, and produces a product having excellent long-term storability of each active ingredient. Further, the chlorella-containing oil and fat food of the present invention contains a large amount of edible oil and fat having an excellent physiologically active action such as cholesterol lowering action, and the raw materials of each component are homogeneously mixed. Therefore, its usefulness as a functional health food is extremely high.
また、比較的水分含有量の多い強壮薬用植物などのエキ
スを含有していても軟質カプセルを膨潤させることがな
いなどの特有の効果を有する。Further, even if it contains an extract such as a tonic drug having a relatively high water content, it has a unique effect of not swelling the soft capsule.
本発明のクロレラ含有油脂食品は、それ自体機能性健康
食品として食用に供されるのみならず、食品の料理用調
味素材などとしても利用可能であり、さらに製品形態が
携帯に便利なのでキヤンプなどのアウトドアーにおいて
も利用できるなどの利点がある。The chlorella-containing oil and fat food of the present invention is not only used as an edible food as a functional health food itself, but can also be used as a food seasoning material and the like, and since the product form is convenient to carry, such as a cap It has the advantage that it can also be used outdoors.
このように、本発明のクロレラ含有油脂食品は、クロレ
ラ藻体、菌茸類、強壮薬用植物などの有効成分を有効且
つ安定に長期間保持させることができなど、従来製品に
ない特有の効果を有する点でその産業上の利用価値はき
わめて高いものである。Thus, the chlorella-containing oil and fat food of the present invention, such as chlorella algal cells, fungi, can retain active ingredients such as tonic herbs effectively and stably for a long period of time, and has a unique effect not present in conventional products. In terms of its possession, its industrial utility value is extremely high.
Claims (7)
菌茸類の熱水抽出液の濃縮エキスとを包接材を加えて包
接練合し乾燥して得られる混合エキス末原料と、強壮薬
用植物およびクロレラ藻体をビタミンE油の存在下に擦
合して得られるスラリー状原料とを、必要によりレシチ
ンなどを添加した食用油脂と均質に撹拌混合することに
より製造される油性懸濁液を軟質カプセルに充填し封入
することを特徴とするクロレラ含有油脂食品の製造方
法。1. A mixed extract powder raw material obtained by adding a clathrate to a concentrated extract of a hot water extract of Chlorella algal cells and a concentrated extract of a hot water extract of fungi and then kneading and drying. And a slurry-like raw material obtained by rubbing a tonic plant and a chlorella algal body in the presence of vitamin E oil, with an edible oil and fat to which lecithin and the like have been added, if necessary, with an oily property A method for producing a chlorella-containing oil and fat food, which comprises filling a suspension into a soft capsule and enclosing the suspension.
用する請求項第(1)項記載のクロレラ含有油脂食品の
製造方法。2. The method for producing a chlorella-containing oil and fat food according to claim 1, wherein cyclodextrin is used as the clathrate.
ケ、シイタケのうちの少なくとも一種を使用する請求項
第(1)項記載のクロレラ含有油脂食品の製造方法。3. The method for producing a chlorella-containing oil and fat food according to claim 1, wherein at least one kind of Pleurotus cornucopiae, Pleurotus cornucopiae, and Shiitake mushroom is used as the fungi.
参、アロエ、梅、ウコギ、ハトムギのうちの少なくとと
も一種を使用する請求項第(1)項記載のクロレラ含有
油脂食品の製造方法。4. The method for producing a chlorella-containing oil and fat food according to claim 1, wherein at least one of garlic, ginseng, aloe, plum, ukulem and adlay is used as a tonic drug.
(1)記載のクロレラ含有油脂食品の製造方法。5. The method for producing a chlorella-containing fat and oil food according to claim 1, wherein the inclusion clathration is carried out under heating and reduced pressure.
胚芽油、菜種油、大豆油、ヤシ油、サフラワー油、ミツ
ロウのうちの少なくとも一種を使用する請求項第(1)
項記載のクロレラ含有油脂食品の製造方法。6. The use of at least one of peanut oil, sesame oil, wheat germ oil, rapeseed oil, soybean oil, coconut oil, safflower oil and beeswax as edible oil and fat (1).
Item 10. A method for producing a chlorella-containing oil or fat food according to Item.
と、菌茸類の熱水抽出液の濃縮エキスとを包接材を加え
包接練合し乾燥して得られる混合エキス末原料と、強壮
薬用植物およびクロレラ藻体をビタミンE油の存在下に
擦合して得られるスラリー状原料とを、必要によりレシ
チンなどを添加した食用油脂と均質に撹拌混合すること
により製造される油性権濁液を軟質カプセルに充填し封
入することにより製造されることを特徴とするクロレラ
含有油脂食品。7. A mixed extract powder raw material obtained by adding a clathrate to a concentrated extract of a hot water extract of Chlorella algal cells and a concentrated extract of a hot water extract of fungi and then kneading and drying. And a slurry-like raw material obtained by rubbing a tonic plant and a chlorella algal body in the presence of vitamin E oil, with an edible oil and fat to which lecithin and the like have been added, if necessary, with an oily property A chlorella-containing oil and fat food product, which is produced by filling a soft capsule with a suspension liquid and encapsulating it.
Priority Applications (17)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2017699A JPH0722495B2 (en) | 1990-01-30 | 1990-01-30 | Chlorella-containing oil and fat food |
| IL9553490A IL95534A (en) | 1990-01-30 | 1990-08-30 | Production of chlorella-containing fatty oil foodstuff |
| AU62549/90A AU630737B2 (en) | 1990-01-30 | 1990-09-17 | Chlorella-containing fatty oil foodstuff and process for producing the same |
| DE4029551A DE4029551A1 (en) | 1989-09-18 | 1990-09-18 | CHLORELLA ALGAE AND MUSHROOMS CONTAINING FATTOEL FOODSTUFFS AND METHOD FOR THE PRODUCTION THEREOF |
| DE4029552A DE4029552A1 (en) | 1989-09-18 | 1990-09-18 | CHLORELLA ALGAE AND MUSHROOMS CONTAINING FATTOEL FOODSTUFFS AND METHOD FOR THE PRODUCTION THEREOF |
| FR9011489A FR2652235B1 (en) | 1989-09-18 | 1990-09-18 | PROCESS AND PRODUCTION OF A FATTY FOOD OIL CONTAINING CHLORELLA ALGAE. |
| DE4029550A DE4029550A1 (en) | 1989-09-18 | 1990-09-18 | CHLORELLA ALGAE AND MUSHROOMS CONTAINING FATTOEL FOODSTUFFS AND METHOD FOR THE PRODUCTION THEREOF |
| GB9020358A GB2236655B (en) | 1989-09-18 | 1990-09-18 | Process for producing encapsulated foodstuff |
| DE4029549A DE4029549A1 (en) | 1989-09-18 | 1990-09-18 | CHLORELLA ALGAE AND MUSHROOMS CONTAINING FATTOEL FOODSTUFFS AND METHOD FOR THE PRODUCTION THEREOF |
| CH3024/90A CH680334A5 (en) | 1990-01-30 | 1990-09-18 | |
| IT02162090A IT1243432B (en) | 1990-01-30 | 1990-10-02 | FOOD BASED ON FAT OILS CONTAINING CHLORELLA AND PROCEDURE TO PRODUCE IT |
| KR1019900015885A KR930007535B1 (en) | 1990-01-30 | 1990-10-06 | Oil containing chlorella |
| FR919110526A FR2663203B1 (en) | 1990-01-30 | 1991-08-22 | PROCESS AND PRODUCTION OF A FATTY FOOD OIL CONTAINING CHLORELLA ALGAE. |
| GB9309857A GB2265072B (en) | 1990-01-30 | 1993-05-13 | Process for producing encapsulated foodstuff |
| SG3494A SG3494G (en) | 1989-09-18 | 1994-01-11 | Process for producing encapsulated foodstuff |
| HK24/94A HK2494A (en) | 1989-09-18 | 1994-01-13 | Process for producing encapsulated foodstuff |
| HK67494A HK67494A (en) | 1990-01-30 | 1994-07-14 | Process for producing encapsulated foodstuff |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2017699A JPH0722495B2 (en) | 1990-01-30 | 1990-01-30 | Chlorella-containing oil and fat food |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH03224443A JPH03224443A (en) | 1991-10-03 |
| JPH0722495B2 true JPH0722495B2 (en) | 1995-03-15 |
Family
ID=11951043
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017699A Expired - Lifetime JPH0722495B2 (en) | 1989-09-18 | 1990-01-30 | Chlorella-containing oil and fat food |
Country Status (9)
| Country | Link |
|---|---|
| JP (1) | JPH0722495B2 (en) |
| KR (1) | KR930007535B1 (en) |
| AU (1) | AU630737B2 (en) |
| CH (1) | CH680334A5 (en) |
| FR (1) | FR2663203B1 (en) |
| GB (1) | GB2265072B (en) |
| HK (1) | HK67494A (en) |
| IL (1) | IL95534A (en) |
| IT (1) | IT1243432B (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0832233B2 (en) * | 1990-03-23 | 1996-03-29 | 美穂 田中 | Oil and fat foods containing active ingredients such as chlorella and method for producing the same |
| JPH0722493B2 (en) * | 1989-09-18 | 1995-03-15 | 美穂 田中 | Chlorella-containing oil and fat food and method for producing the same |
| JPH0722494B2 (en) * | 1989-09-25 | 1995-03-15 | 美穂 田中 | Chlorella-containing oil and fat food and method for producing the same |
| GB2274235B (en) * | 1993-01-13 | 1996-06-26 | Neo Life Company Of America | Carotenoid food supplement |
| DK1140104T3 (en) * | 1998-07-31 | 2008-01-21 | Massachusetts Inst Technology | Treatment of Alzheimer's disease by increasing cytidine levels in vivo |
| US8518882B2 (en) | 1998-07-31 | 2013-08-27 | Massachusetts Institute Of Technology | Methods and compositions for ameliorating or inhibiting decline in memory or intelligence or improving same |
| KR100644916B1 (en) * | 2004-09-23 | 2006-11-14 | 웰리네사람들주식회사 | Manufacturing method of edible oil and fat containing extract of Phellinus linteus and the product obtained therefrom |
| JP7804270B2 (en) * | 2020-04-03 | 2026-01-22 | 学校法人近畿大学 | Composition containing lignan compounds, essential oils and fats |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0832233B2 (en) * | 1990-03-23 | 1996-03-29 | 美穂 田中 | Oil and fat foods containing active ingredients such as chlorella and method for producing the same |
| JPH0722493B2 (en) * | 1989-09-18 | 1995-03-15 | 美穂 田中 | Chlorella-containing oil and fat food and method for producing the same |
| JPH0722494B2 (en) * | 1989-09-25 | 1995-03-15 | 美穂 田中 | Chlorella-containing oil and fat food and method for producing the same |
-
1990
- 1990-01-30 JP JP2017699A patent/JPH0722495B2/en not_active Expired - Lifetime
- 1990-08-30 IL IL9553490A patent/IL95534A/en unknown
- 1990-09-17 AU AU62549/90A patent/AU630737B2/en not_active Expired
- 1990-09-18 CH CH3024/90A patent/CH680334A5/de not_active IP Right Cessation
- 1990-10-02 IT IT02162090A patent/IT1243432B/en active IP Right Grant
- 1990-10-06 KR KR1019900015885A patent/KR930007535B1/en not_active Expired - Lifetime
-
1991
- 1991-08-22 FR FR919110526A patent/FR2663203B1/en not_active Expired - Lifetime
-
1993
- 1993-05-13 GB GB9309857A patent/GB2265072B/en not_active Expired - Lifetime
-
1994
- 1994-07-14 HK HK67494A patent/HK67494A/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| JPH03224443A (en) | 1991-10-03 |
| IL95534A (en) | 1994-01-25 |
| GB2265072B (en) | 1994-04-13 |
| IT1243432B (en) | 1994-06-10 |
| FR2663203B1 (en) | 1992-10-30 |
| HK67494A (en) | 1994-07-22 |
| IT9021620A0 (en) | 1990-10-02 |
| CH680334A5 (en) | 1992-08-14 |
| KR910014058A (en) | 1991-08-31 |
| IL95534A0 (en) | 1991-06-30 |
| FR2663203A1 (en) | 1991-12-20 |
| IT9021620A1 (en) | 1992-04-02 |
| AU6254990A (en) | 1991-08-01 |
| GB2265072A (en) | 1993-09-22 |
| GB9309857D0 (en) | 1993-06-23 |
| KR930007535B1 (en) | 1993-08-12 |
| AU630737B2 (en) | 1992-11-05 |
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