JPH0723864B2 - Chromatoskiana - Google Patents

Description

Description: TECHNICAL FIELD The present invention relates to a chromatographic scanner using a thin-layer chromatographic plate or an electrophoretic gel as a sample, and in particular, scans a light beam irradiating the sample in a zigzag and linear manner. It relates to a chromatographic scanner that can be used.

(Prior Art) When performing an ultraviolet, visible or sometimes infrared spectrophotometer or other optical absorption analysis, the wavelength resolution and line width (also called bandwidth) are changed by changing the width and height of the exit slit. ) And the amount of light incident on the sample can be changed. However, in the method using the normal fixed slit,
It is not suitable for sequentially and continuously analyzing and examining the one-dimensional or two-dimensional absorption distribution in the sample or on the sample surface with respect to the spatial direction.

Therefore, as one method, a flying spot method has been proposed in which the incident light on the sample, that is, the irradiation point is fixed and the sample is sequentially moved.

FIG. 3 shows an example of a flying spot type chromatographic scanner.

1a and 1b are light source lamps, 2 is a condenser mirror, 3 is a cut filter, 4 is an entrance slit, 5 is a collimator mirror, 6 is a plane diffraction grating, and 7 is a pulse motor. A rotary slit disk 9 is attached to the rotary shaft of the pulse motor 7, and the rotary slit disk 9 is provided with a spiral slit in which an increment of the distance from the center appears in proportion to the rotation angle. Reference numeral 8 denotes an exit slit assembly, and a rectangular slit 8a that defines the width direction of the exit slit is formed in the slit plate at a position intersecting with the spiral slit of the rotary slit disk 9, and the rotary slit disk 9 is provided. Plural types of rectangular slits 8b having a fixed width and height are provided at positions not overlapping with. The pulse motor 7 and the outlet slit assembly 8 are mounted on a sliding base 10 that can move linearly in the A and B directions indicated by the arrows. 11 is a light shielding plate.

When performing flying spot type zigzag scanning, a rotary slit disk punched with spiral slits
Rotate before and after the elongated rectangular slit 8a (rear in the case shown in the figure) placed parallel to each other on the radius (although a slight air gap is required), and open the hole created at the intersection of the two. The phenomenon that the emitted light flux moves in the height direction of the rectangular slit 8a is used. Then, in order to switch to the measurement method using the fixed slit, the system of the rotary slit disk 9 is completely moved from the optical path position, and the rectangular slit 8b for the fixed slit is used.
Either of them is positioned at the optical path position.

(Problems to be Solved by the Invention) In the chromatographic scanner shown in FIG. 3, when the diameter of the rotating slit disk 9 becomes large, the rotary slit disk 9 is moved linearly over a considerable distance when switching from the flying spot method to the fixed slit method. Since it must be done, it can be seen that the space loss is large in terms of mechanical design. In addition, when the outlet slit assembly 8 having the fixed slits is attached to the sliding base 10, in order to avoid "hit" with other surrounding mechanical parts, in some cases, an unreasonable state, that is, complete There is a possibility that it will be installed in a state where it cannot absorb the effects of vibration and bending.

The rotary slit disk 9 can be rotated only in one direction. Therefore, in the flying spot type zigzag scanning, there are a portion where light passes through the spiral slit and a portion where light does not pass at all because it is blocked by the rotary slit disk 9. Therefore, inconveniences in photometry such as a sudden change in the negative high voltage level of the photomultiplier tube of the detector and an abnormal response of the photometry signal occur.

An object of the present invention is to prevent inconvenience in photometry in a flying spot type zigzag scanning, and to switch between a flying spot type and a fixed slit type, a rotary slit disk and a fixed slit and their surroundings. This is to eliminate the need for a sliding type moving mechanism that is complicated and requires a lot of adjustment man-hours so that the mechanical portion does not have to be slid.

(Means for Solving the Problems) In the chromatographic scanner of the present invention, a spiral slit in which an increase in the distance from the center appears in proportion to the rotation angle is formed in a substantially semicircular region, and in the remaining semicircular region. Spectrometer with a rotating slit disk with a rectangular slit and a width direction slit that defines the width direction of the light flux and rotates around the rotation axis to be installed and removed in the optical path position. Provide at the exit position of. When performing a flying spot type zigzag scanning, the spiral slit of the rotary slit disk and the width direction slit are installed in the optical path position, and the rotation is performed within a range where a light flux passes through the spiral slit. Rotate the slit disk back and forth,
When performing a fixed slit scanning, the rotary slit disk is fixed so that a predetermined rectangular slit of the rotary slit disk is installed in the optical path, and the width direction slit is removed from the optical path position or the optical path. Be sure to keep it in place.

(Embodiment) FIG. 1 shows an embodiment of the present invention. FIG. 1A is a plan view and FIG. 1B is a front view.

The rotary slit disk 12 is attached to a support plate and attached to the rotary shaft of the disk drive motor 7. A pulse motor is suitable as the disk drive motor 7.

A spiral slit 13 is punched in the semicircular region of the rotary slit disk 12. The spiral slit 13 has a position where the X-axis (horizontal axis in the drawing) intersects with the spiral slit 13 when the rotary slit disk 12 rotates clockwise in the drawing.
The radius from the origin, which is the center, is r = r ₁ + (r ₂ −r ₁ ) θ / 180. Reference numeral 15 is a rotation angle origin detection hole, and θ is an angle (degree) counterclockwise from the position where the rotation angle origin detection hole 15 is provided.

When the rotary slit disk 12 turns clockwise, the size of r increases toward r ₁ → r ₂ , and then when it turns counterclockwise, it decreases toward r ₂ → r ₁ . From this, Y in the figure
The position of y = rm = (r ₁ + r ₂ ) / 2 on the axis (vertical axis) is set to the position of the optical axis (optical path position) that passes through the exit slit of the spectrometer, and the rotary slit disk 12 is approximately ± 90 °. When rotated alternately in both the forward and reverse directions at a rotation angle of, the position of the light flux leaking from the spiral slit 13 within the range from y = r ₁ to y = r ₂ undergoes periodic oscillation. Further, during the rotation of the rotary slit disk 12, the light passing through the rotary slit disk 12 is not completely blocked.

Further, four kinds of rectangular slits 14-1 to 14-4 having fixed width (circumferential direction) and height (radial direction) are punched in the remaining semicircular region of the rotary slit disk 12. There is. The rectangular slits 14-1 to 14-4 are arranged at the same central angle.

To select one of these rectangular slits 14-1 to 14-4, the rotation angle origin detection hole 15 crosses the groove of the photocoupler (not shown) to detect the origin, and then the required pulse from there. The number is sent to the pulse motor 7 for driving the disk to position the rectangular slits 14-1 to 14-4 having a specific width and height at the optical path position. This operation can be performed by inputting from the keyboard of the operation unit connected to the main device.

Since the slit holes of different patterns are punched in each semi-circular area of the rotating slit disk 12, the supporting disk to which the rotating slit disk 12 is attached does not have a mass deviation for both areas, and the balance is well balanced. Relief holes (holes other than the slit part) so that the rotating slit disk 12 can be taken off normally
Punch out.

Numeral 16 is a slit for the width direction, which is attached near the tip of the support plate 18. The base end of the support plate 18 is attached to the drive motor 22 by a boss 20 by a direct axis. As a result, the width direction slit 16 moves between the optical path position shown by the solid line and the position deviated from the optical path shown by the broken line by the drive motor 22 operating and the support plate 18 turning. It will be possible. A pulse motor is also suitable as the drive motor 22.

A pulse motor is used as the drive motor 22 in order to ensure the positional accuracy when the width direction slit 16 is inserted in the optical path position, but the insertion position cannot be reliably held only by the excitation torque of the pulse motor. In case, the boss
20 is provided with two pins 24 and 26, one pin 24 is made to hit the hit pin 28 when the width direction slit 16 is inserted in the optical path, and the other pin 26 is pulled by the tension spring 30. So that the pin 24 is pressed against the pin 28. As a result, the play in the direction in which the width direction slit 16 and the support plate 18 rotate around the axis can be eliminated.

Reference numeral 32 is a photosensor for detecting the origin of the rotation angle of the support plate 18 of the slit 16.

In the present embodiment, when performing scanning in a mode in which the irradiation light beam on the sample plate oscillates left and right, that is, when performing flying spot scanning, the intersection of the spiral slit 13 of the rotary slit disk 12 and the width direction slit 16 as shown in the figure. Light flux to pass through. Then, the rotary slit disc 12 is rotated counterclockwise and clockwise about the position shown in the figure alternately by the same angle. At this time, the spiral slit 13
Is the focus surface in the vertical direction, that is, in the height direction,
The width direction slit 16 serves as a focus surface in the width direction.

Next, when performing the fixed slit type scanning, one of the rectangular slits 14-1 to 14-4 is inserted in the optical path.

In that case, when the widthwise opening of the selected rectangular slit is larger than the opening of the slit 16, the slit 16 becomes an obstacle. Therefore, from the spiral slit 13 to the square slit
After switching to 14-1 to 14-4, the drive motor 22 is operated to automatically remove the slit 16 from the optical path.

Further, if the play existing in the axial direction of the drive motor 22 exceeds the positional accuracy of the slit 16 with respect to the light beam passage direction, that is, the tolerance, the rotary shaft of the drive motor 22 is made to slightly project from the support plate 18, It is necessary to press it by some means from the front side of the drawing in FIG. 1 (B) to the back side. However, do not press it too strongly. If the drive motor 22 is a double shaft type, the shaft may be pushed out from the side opposite to the side to which the support plate 18 is attached.

FIG. 2 shows another embodiment.

Compared with the embodiment of FIG. 1, the rotary slit disk 34 is provided with the same spiral slit 13 as the rotary slit disk 12 of FIG. 1, but four square slits 14-1 to 14- Four
Instead, three rectangular slits 36-1 to 36-3 for changing only the height of the fixing slit are provided.

On the other hand, regarding the slits for the width direction, the supporting plate in FIG.
A disc 38 is provided in place of 18, and the disc 38 has three width-direction slits 40-1 to 40- with different width-direction openings.
3 is provided and the center of the disk 38 is attached to the rotary shaft of the drive motor 22, so that any of the width direction slits 40-1 to 40-3 is placed in the optical path.

In this embodiment, the spiral slit 13 and the width direction slit 40-
By combining 1 to 40-3, three kinds of slit dimensions can be obtained by the flying spot method, and also the rectangular slits 36-1 to 36-3 for the height direction and the slits 40-1 to 40 for the width direction.
Nine kinds of slit dimensions can be obtained by the fixed slit method by the combination of -3.

However, at this time, the focus surface in the height direction must be on the surface of the rotary slit disk 34, and the focus surface in the width direction must be on the disk 38.

In the present embodiment, the disc 38 may be fan-shaped.

If the occupying central angle of the spiral slit 13 in the rotary slit disk 12 or 34 is small, two or more spiral slits having different slit widths can be provided in the semicircular region. Thereby, the height direction of the irradiation light beam on the sample plate can be made variable even in the flying spot scanning mode.

(Effects of the Invention) According to the present invention, the following effects can be achieved.

(1) During measurement by the flying spot method, reciprocal rotation is performed alternately in the forward and reverse directions only within the range in which the spiral slit is provided, so the light beam is completely cut off in the middle, or the square hole of the fixing slit It does not cross the luminous flux. Therefore, the photometric system is not adversely affected by a sudden change in the negative high voltage level of the photomultiplier tube or an abnormal response of the photometric signal.

(2) Since switching from the flying spot type slit to the fixed slit can be performed only by controlling the rotation of the rotary slit disc, the entire mechanism of the rotary slit disc including the motor is linearly moved. You don't have to let me. And a complicated mechanism for such movement is unnecessary. It also reduces costs.

(3) Since the width direction slit is moved in and out of the optical path by rotating a support plate or a disk provided with the width direction slit about a rotation axis, the width direction slit is in the same direction as the width direction. It becomes easy to obtain the position reproducibility.

[Brief description of drawings]

FIG. 1 shows an embodiment of the present invention. FIG. 1A is a plan view and FIG. 1B is a front view. FIG. 2 is a front view showing another embodiment, and FIG. 3 is a plan view showing a conventional chromatographic scanner. 12,34 ... Rotating slit disk, 13 ... Spiral slit, 14-1 to 14-4,36-1 to 36-3 ... Square slit, 18 ... Support plate, 38 ... Disc.

Claims (1)

[Claims] 1. A rotary slit disk in which a spiral slit in which an increase in distance from the center appears in proportion to a rotation angle appears in a substantially semicircular region, and a rectangular slit is formed in the remaining semicircular region, The width direction of the light beam is regulated, and a slit for width direction that rotates around the rotation axis and is installed and removed in the optical path position is provided close to each other at the exit position of the spectroscope, and flying spot type zigzag scanning is performed. When performing the above, the spiral slit of the rotary slit disc and the slit for the width direction are installed at the optical path position, and the rotary slit disc is reciprocally reciprocally rotated within a range where a light beam passes through the spiral slit. When performing the fixed slit type scanning, the rotary slit disc is fixed so that a predetermined rectangular slit of the rotary slit disc is installed in the optical path, and Chromatographic scanner to be installed in removing or optical path position lit from the optical path position.