JPH0741066B2 - Blood component separation bag and blood component separation method - Google Patents

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a blood component separation bag for fractionating blood collected by a donor into highly pure components such as red blood cells, platelets and plasma, and a method for separating blood components using the same. .

[0002]

2. Description of the Related Art FIG. 4 is a schematic view of a blood component separation bag 61 currently in use. Blood component separation bag 61
Is composed of a blood collection bag 62 and a plurality of separation bags 63, 64, 65 connected thereto.

In use, blood is collected from a blood donor through a blood collection needle 67 and a blood collection tube 68 into a blood collection bag 62, and subjected to a strong centrifugation process to obtain a platelet poor plasma layer (hereinafter referred to as "PP").
P ”), buffy coat layer, red blood cell layer (hereinafter“ CR ”)
C "), and each component of blood was separated and collected by the following operation procedure. PPP connection tubes 73, 75, 78
A step of separating into the separation bag 65 via the step of transferring the buffy coat layer into the separation bag 63 via the connecting tubes 73 and 79,

The step of adding the red blood cell preservation solution enclosed in the separation bag 64 to the red blood cells remaining in the blood collection bag 62 through the connecting tubes 77, 75 and 73, and the buffy coat layer in the separation bag 63 is subjected to a light centrifugation. Separation treatment to separate the platelet layer (upper layer) from white blood cells / red blood cells (lower layer), the step of separating the platelets into the separation bag 64 via the connecting tubes 79, 75, 77, and In the blood collection bag 62, red blood cell preservation solution added red blood cells,
Platelets are collected in the separation bag 64, PPP is collected in the separation bag 65, and white blood cells and red blood cells are collected in the separation bag 63. The white blood cells / red blood cells and minute plasma in the separation bag 63 were further manipulated and used to prepare interferon and the like.

[0005]

After the strong centrifugation, the PPP and the buffy coat layer are transferred to the separation bags 65 and 63. At that time, the PPP and the buffy coat layers are placed in the connecting tubes 75 and 78.
In the connecting tubes 73 and 79, the buffy coat layer (C
(It may be RC). The buffy coat layer in the connecting tube 79 is washed into the separating bag 63 with an appropriate amount of PPP from the separating bag 65. At that time, white blood cells, red blood cells, platelets, etc. in the buffy coat layer are contained in the connecting tube 79. Blood cell components remain.
The remaining blood cell components will be transferred together with the red blood cell preservation solution in the connecting tube 75 or 77 when the platelets are transferred to the separation bag 64 after lightly centrifuging the buffy coat layer of the separation bag 63. In the platelets prepared in 64, blood cell components such as red blood cells and white blood cells and red blood cell preservation solution are contained as impurities. It is an object of the present invention to provide a method for preventing blood components and a red blood cell preservative solution from mixing into platelets, and a blood component separation bag.

[0006]

According to the present invention, a connecting tube 1 is attached to a blood collecting bag 2 (32) in which a blood preservation solution is enclosed.
The buffy coat separating bag 3 (33) is connected through the connecting tubes 21 (5) to the separating bag 3 (33) via the connecting pipes 14 (44).
The bag 6 for separating white blood cells or the like or the bag 36 for separating platelets is connected via 1), and the connecting pipe 14 (44)
Is connected to the separation bag 4 (34) in which the erythrocyte preservation solution is enclosed via the connecting tubes 15 (45) and 17 (47) and the connecting tube 16 (46), and the connecting tube 18 (48) is connected. The blood plasma separation bag 5 (35) is connected via the connecting tube 13, 19, 21 (43, 49, 51) and the connecting pipe 14 (44) downstream of the blood collecting bag 2 (32). , The buffy coat separation bag 3 (33), the white blood cell separation bag 6 or the platelet separation bag 36 are sequentially arranged, and the connecting tubes 15 and 1 are provided downstream of the buffy coat separation bag 3 (33).
8 (45, 48) for plasma separation bag 5 (3
The blood component separation bag 1 (31) in which 5) is arranged is provided.

The present invention also provides a method of separating blood components using the blood component separation bag 1 (31) described in the above invention, which comprises the following steps. A step of centrifuging a blood collection bag 2 (32) containing whole blood to separate it into a platelet poor plasma layer (upper layer), a buffy coat layer (interface), and an erythrocyte layer (lower layer). Connection tube 13 (43), 1
5 (45), 18 (48), and collecting into the plasma separation bag 5 (35), the buffy coat layer is connected to the connecting tube 13 (43),
Collecting into the separation bag 3 (33) via 19 (49), centrifuging the buffy coat layer in the separation bag 3 (33) to concentrate platelet layer (upper layer) and leukocyte / red blood cell layer A step of separating into a (lower layer), a step of separating the white blood cell / red blood cell layer into a separation bag 6 through a connecting tube 21, and collecting platelets in the separation bag 3.

Further, in the present invention, in place of the steps (1) to (3) described in the above invention and the steps described in the above invention, the white blood cell / red blood cell layer and the like are left in the buffy coat separation bag 33 and the platelet layer is connected. The present invention provides a method of separating blood components, which comprises a step of collecting the blood component in the separation bag 36 via the tube 51.

[0009]

When the platelets are separated and collected in the separation bag 1, high purity platelets can be collected because other blood cell components and red blood cell preservation solution are not present in the connecting tube or the like between them.

[0010]

1 is a schematic view of a blood component separation bag 1 of the present invention. The blood component separation bag 1 is a blood collection bag 2
And a plurality of separation bags 3, 4, 5, and 6 connected to this via a connecting tube and a connecting tube.

A blood collection tube 8 having a blood collection needle 7 connected to the tip thereof, a blood transfusion port 9 capped by a protector 10 and a connection tube 12 loaded with a communication piece 11 are connected to the upper end of the blood collection bag 2. . A blood preservation solution is enclosed in the blood collection bag 2.

A buffy coat separating bag 3 is connected downstream of the blood collecting bag 2 via a connecting tube 13, a connecting tube 14 and a connecting tube 19. The lower part of the separation bag 3 is formed in a funnel shape, and the discharge tube 24 loaded with the communication piece 20 is connected to the lowermost end part thereof. Furthermore, a separation bag 6 for separating white blood cells and the like is connected to the separation bag 3 via a connecting tube 21. Since the separation bag 6 only separates and collects unnecessary minute components such as white blood cells, it may have a small capacity.

Further, downstream of the separation bag 3, a separation bag 4 containing a red blood cell preservation solution and a plasma separation bag 5 are connected. The separation bag 4 is connected to the connecting pipe 14 via a connecting tube 15, a connecting pipe 16, a connecting tube 17, and a connecting tube 22 in which a communicating piece 23 is loaded. The separating bag 5 is connected to the connecting pipe 16 via a connecting tube 18.

Next, a method of using the blood component separation bag 1 (a method of separating blood components) will be described. The donor is punctured with the blood collection needle 7, and blood is collected in the blood collection bag 2 through the blood collection tube 8. The collected blood is subjected to strong centrifugation to separate into a PPP (upper layer) buffy coat layer (interface layer containing white blood cells and platelets) and a CRC (lower layer).

The communication piece 12 is cut to form the upper layer PPP.
Is separated into the separation bag 5 through the connecting tube 12, the connecting tubes 13, 15, and 18. Then, the buffy coat layer is separated into the separation bag 3 through the connecting tubes 13 and 19.

The connecting tube 19 is sealed and the separating bags 3 and 6 are separated from the blood collecting bag 2 and the separating bags 4 and 5. Thereafter, the buffy coat layer in the separation bag 3 is subjected to a light centrifugation treatment to separate it into platelets (upper layer) and leukocyte / red blood cell layers (lower layer). Then, the communication piece 20 is cut, and the white blood cell / red blood cell layer is separated and collected in the separation bag 6 through the discharge tube 24. Since the interface layer between platelets (upper layer) and white blood cells / red blood cells (lower layer) becomes longer in the small-diameter drain tube, separation is facilitated. Finally, high-purity platelets in which the red blood cell preservative solution is not mixed and the red blood cells and white blood cells are completely removed can be collected in the separation bag 3.

Further, in the discharge tube 24 provided between the separation bag 3 and the separation bag 6, the communication piece 2 is placed.
0 does not necessarily have to be arranged. At the time of performing the light centrifuge treatment of the stand and the buffy coat, the centrifuge treatment is performed using the centrifuge bucket 25 loaded with the exclusive fixing member 26 capable of fixing the separation bags 3 and 6 as shown in FIG. At the same time, platelets and white blood cells / red blood cells are separated. After centrifugation, platelets (upper layer) and white blood cells / red blood cells (lower layer) are fractionated in the separation bag 6, and the platelets are sorted into the separation bag 3 via the connecting tube 21. High-purity platelets free of erythrocytes and leukocytes without being mixed with the erythrocyte preservation solution can be collected in the separation bag 3.

FIG. 3 shows another blood component separating bag 31.
FIG. In the blood component separation bag 31, a platelet discharge tube 54 is provided at the upper end of the buffy coat separation bag 33, and the separation bag 3 is provided via the connecting tube 51.
6 is connected.

In use, the buffy coat collected in the separation bag 33 is separated into platelets (upper layer) and white blood cells / red blood cells (lower layer) by light centrifugation, and the platelets are separated through the discharge tube 54 and the connecting tube 51. It collects in the bag 36. In the separation bag 36, high-purity platelets free of red blood cell preservative and free of white blood cells and red blood cells can be collected.

P collected in the separation bag 5 (35)
PP can be separated individually by seal cutting the connecting tube 18 (38), and remains in the blood collection bag 2 (32).
C added the red blood cell preservation solution in the separation bag 4 (34),
The connection tube 13 (33) can be separated by sealing cutting. On the other hand, the white blood cells and red blood cells remaining in the separation bag 6 or the buffy coat separation bag 33 are further manipulated to be used for adjusting interferon and the like.

[0021]

INDUSTRIAL APPLICABILITY As described above, according to the present invention, platelet poor plasma, platelets and red blood cells can be efficiently separated and collected, and in particular, highly pure platelets can be separated and collected.

[Brief description of drawings]

FIG. 1 is a schematic view of a blood component separation bag of the present invention.

FIG. 2 is a schematic view showing an example of a method for separating platelets.

FIG. 3 is a schematic view showing another embodiment of the blood component separation bag.

FIG. 4 is a schematic view of a conventional blood component separation bag.

[Explanation of sign]

 1, 31 Blood component separation bag 2, 32 Blood collection bag 3, 33 Buffy coat separation bag 4, 34 Separation bag filled with erythrocyte preservation solution 5, 35 Plasma separation bag 6 Separation bag for leukocytes, etc. 7, 37 Blood collection needle 8, 38 Blood collection tube 9, 39 Blood transfusion port 10, 40 Protector 11, 41 Connection tube 12, 42 Communication piece 13, 43 Connection tube 14, 44 Connection tube 15, 45 Connection tube 16, 46 Connection tube 17 , 47 connecting tube 18, 48 connecting tube 19, 49 connecting tube 20, 50 connecting piece 21, 51 connecting tube 22, 52 connecting tube 23, 53 connecting piece 24, 54 discharge tube 25 centrifuge bucket 36 bag for separating platelets

Claims (3)

[Claims] 1. A blood collection bag 2 in which a blood preservation solution is enclosed.
In (32), connecting tubes 13 (43), 19 (3
9), the buffy coat separation bag 3 (33) is connected via the connection pipe 14 (44), and the separation bag 3
The separation bag 6 for separating white blood cells or the like or the separation bag 36 for platelets is connected to (33) via the connection tube 21 (51), and the connection tube 15 is connected to the connection tube 14 (44).
(45), 17 (47) and the connecting bag 16 (46) are connected to the separation bag 4 (34) in which the erythrocyte preservation solution is sealed, and plasma is separated through the connecting tube 18 (48). Bag 5 (35) is connected, and connecting tubes 13, 19, 2 are provided downstream of the blood collecting bag 2 (32).
1 (43, 49, 51) and the connecting pipe 14 (44) in order to sequentially separate the buffy coat separation bag 3 (33), the white blood cell separation bag 6 or the platelet separation bag 3.
6 is arranged, and the buffy coat separating bag 3 (3
A blood component separation bag 1 (31), characterized in that a plasma separation bag 5 (35) is arranged downstream of 3) via connecting tubes 15, 18 (45, 48). 2. A method for separating a blood component using the blood component separation bag 1 (31) according to claim 1, which comprises the following steps. A step of centrifuging a blood collection bag 2 (32) containing whole blood to separate it into a platelet poor plasma layer (upper layer), a buffy coat layer (interface), and an erythrocyte layer (lower layer). Connection tube 13 (43), 1
5 (45), 18 (48), and collecting into the plasma separation bag 5 (35), the buffy coat layer is connected to the connecting tube 13 (43),
Collecting into the separation bag 3 (33) via 19 (49), centrifuging the buffy coat layer in the separation bag 3 (33) to concentrate platelet layer (upper layer) and leukocyte / red blood cell layer A step of separating into a (lower layer), a step of separating the white blood cell / red blood cell layer into a separation bag 6 through a connecting tube 21, and collecting platelets in the separation bag 3. 3. The steps (1) to (3) according to claim 2;
Instead of the steps described above, a step of leaving the white blood cell / red blood cell layer and the like in the buffy coat separation bag 33 and collecting the platelet layer into the separation bag 36 via the connecting tube 51 is characterized in that A method for separating blood components.