JPH075448B2 - Cosmetics - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial field of application] The present invention relates to cosmetics, more specifically, a high moisturizing effect,
The present invention relates to cosmetics having excellent feeling of use.

[Conventional technology]

Among the properties that a cosmetic composition should have, the moisturizing property is particularly important in order to prevent irritation from the outside and rough skin when the cosmetic composition is applied to the skin and to improve the usability. Various studies have heretofore been made to impart moisturizing properties to cosmetics. For example, blending cosmetics with polyols such as sorbitol, glycerin, and 1,3-butylene glycol; blending skin components such as lactate, pyrrolidonecarboxylate, hyaluronic acid; animal and plant extracts, lactic acid bacteria culture fluid, etc. Blending; other blending of urea is carried out [Fragrance Journal No.79,
64-71 (1986)].

[Problems to be solved by the invention]

However, the moisturizing effect of the conventional moisturizers is not yet sufficient, and the cosmetics containing these moisturizers are not satisfactory in use.

[Means for solving problems]

In view of such an actual situation, the present inventors have conducted various studies in order to solve the above problems, and it is possible to obtain an excellent moisturizing effect and a feeling of use by using hyaluronic acid or a salt thereof and a lactic acid bacterium culture solution in combination. The inventors have found out and completed the present invention.

That is, the present invention provides a cosmetic composition containing hyaluronic acid or a salt thereof and a lactic acid bacterium culture solution.

Hyaluronic acid or a salt thereof to be added to the cosmetic of the present invention includes those extracted from animal tissues such as chicken mackerel, Streptococcus zooepidemicus (Streptoc).
occus zooepidemicus), Streptococcus pyogenes, etc. obtained by culturing bacteria belonging to the genus Streptococcus can be used. Among them, hyaluronic acid or a salt thereof having a molecular weight of 2,000,000 or more, which the applicant of the present invention has previously found and applied for a patent (Japanese Patent Application No. 61-99446) is preferable. The hyaluronic acid having a molecular weight of 2,000,000 or more is produced, for example, by culturing a microorganism producing the hyaluronic acid in a nutrient medium and collecting it from the culture.

In the above method, as a microorganism that produces a hyaluronic acid having a molecular weight of 2 million or more, a bacterium belonging to the genus Streptococcus, which has the hyaluronic acid-producing ability, and which is non-hyaluronidase-hyaluronidase-producing, for example, as follows. One mutant strain of Streptococcus zooepidemicus obtained is mentioned. That is, first, Streptococcus zooepidemicus belonging to the Lancefield serogroup C, which has a strong ability to produce hyaluronidase (hyaluronan degrading enzyme) from the nasal mucosa and produces hyaluronan (the identification of this bacterium is the Barjay's Manual of -Determinative Bacteriology 8th Edition, 1974). This strain is called MacLennan,
J.Gen.Microbiol., 14,134-142,1956) pointed out that hyaluronic acid was well produced under aerobic conditions, and when glucose was used as a carbon source, 2 g / hyaluronic acid was obtained by adding 4% glucose. It was produced (yield of sugar relative to hyaluronic acid was 5%). The molecular weight of hyaluronic acid obtained at this time was 300,000 to 600,000. This strain is routinely used (bacterial / phage genetic experiment method, protein / nucleic acid enzyme separate volume, Kyoritsu Publishing
1972) treated with ultraviolet rays or chemical agents (N-methyl-N'-nitro-N-nitrosoguanidine (NTG), ethyl methane sulfonic acid, etc.), and the treated bacterial cells were diluted with blood agar medium. , Collect a colony that does not show hemolysis,
Next, after mutating this strain again, it was applied to a nutrient agar medium containing hyaluronic acid, and a colony that did not decompose hyaluronic acid was collected to obtain Streptococcus
1 mutant strain of Zooepidemicus was obtained (Reference Example 1 in the following article)
reference).

The thus obtained mutant strain 1 of Streptococcus zooepidemicus (hereinafter referred to as "this strain") forms a transparent colony having extremely strong viscosity on Todd Hewittbroth agar medium and is non-hemolytic ( β-
Hemolytic: Negative), hyaluronidase non-producing, Streptococcus belonging to Lancefield serogroup C, and the mycological properties of this bacterium are as follows.

(A) Gram stainability: positive (b) 10 ° C growth: negative (c) 45 ° C growth: negative (d) 0.1% methylene blue resistance: negative (e) 6.5% salt resistance: negative (f) 40 % Bile resistance: Negative (g) Bacitracin resistance: Positive (h) pH9.6 Resistance: Negative (i) 60 ° C, 30 minutes Resistance: Negative (j) Gelatin degradability: Negative (k) Starch degradability : Positive (l) Sodium hippurate degradability: Negative (m) Esculin degradability: Weakly positive (n) Arginine degradability: Positive (o) Sugar fermentation: Glucose, galactose, sucrose, lactose, maltose, sorbitol and salicin Is positive, glycerin, mannitol, trehalose and arabinose are negative.

The present applicant named this bacterium Streptococcus zooepidemicus YIT2030 based on the mycological properties of this bacterium, and deposited it at the Institute for Microbial Technology, National Institute of Advanced Industrial Science and Technology as Microtechnology Research Institute No. 1305.

Next, a method for culturing the present bacterium and collecting hyaluronic acid having a molecular weight of 2,000,000 or more from the culture will be described.

The medium for culturing the bacterium to obtain hyaluronic acid preferably contains a carbon source, an organic / inorganic nitrogen source, and, if necessary, an inorganic salt or an organic trace nutrient. The carbon source is preferably glucose, galactose, sucrose, lactose, fructose, maltose, sorbitol, those containing sugars such as starch hydrolysates,
Alternatively, organic acids, aliphatic alcohols, etc. may be used. The nitrogen source may be a general material regardless of whether it is organic or inorganic, but various meat extracts, amino acid mixtures, peptone, yeast extract and the like are preferable. In addition, sodium, potassium, calcium,
Chlorides such as magnesium and iron, sulfates, phosphates, nitrates, carbonates and vitamins may be added as required.

Aerobic conditions are essential for culturing, and it is preferable to increase the stirring speed according to the increase in the viscosity of the culture solution, but excessive stirring is not preferable. The culturing temperature is preferably 25 to 38 ° C at which the bacterium grows. Further, during culturing, the bacterium produces lactic acid, and the lactic acid inhibits the growth of the bacterium and the production of hyaluronic acid. Therefore, an alkaline aqueous solution is added to neutralize the lactic acid, and the pH of the culture solution is adjusted to 6-. It is preferably adjusted within the range of 8. Examples of the aqueous alkaline solution used at this time include aqueous solutions of sodium hydroxide and potassium hydroxide and aqueous ammonia.

This bacterium is a strain that produces high molecular weight hyaluronic acid (molecular weight of 2,000,000 or more) at an extremely high yield and production rate, and particularly good results are obtained when glucose is used as a carbon source. That is, when glucose is added, the target hyaluronic acid production rate is significantly improved, and the addition amount is 0.5-
6% is preferable.

Since this bacterium does not accumulate substances other than hyaluronic acid in the culture medium as polymeric substances, it is easy to separate and purify hyaluronic acid accumulated in the culture medium after culturing, and to separate and purify already known polysaccharides. The method may be used.

An example of a method for separating and purifying hyaluronic acid having a molecular weight of 2,000,000 or more is shown. The culture solution is diluted with water so as to have an appropriate viscosity (preferably 100 centipoise or less), and the pH is adjusted to 4 or less with trichloroacetic acid. Then, the cells are separated and removed by centrifugation or membrane filtration (pore size 0.2 μm or less). Next, the low-molecular substances dissolved in the solution are removed by ultrafiltration, dialysis, an organic solvent precipitation method or an adsorption method using an ion exchange resin, etc., followed by an organic solvent precipitation method, freeze drying or spray drying. Hyaluronic acid having a molecular weight of 2,000,000 or more can be obtained by using a means.

Regarding hyaluronic acid obtained by extracting and purifying from the above culture solution in this manner, a hyaluronic acid standard (molecular weight of about 630,000, Si
As a result of various investigations in comparison with (produced by gma), it was confirmed that this product was hyaluronic acid. The properties are shown below.

(1) Electrophoresis using a cellulose acetate membrane shows the same mobility as the standard product.

(2) When it is decomposed by actinomycetes hyaluronidase (manufactured by Amano Pharmaceutical Co., Ltd.) and the decomposed product is subjected to silica gel thin layer chromatography, two spots appear with the same mobility as that of the standard decomposed product after the treatment.

(3) When the chemical composition is analyzed, N-acetyl-D-glucosamine and D-glucuronic acid are present in a molar ratio of 1: 1.

(4) Specific optical rotation is [alpha ▲] ²⁰ _D ▼ = -69 °.

(5) The infrared absorption spectrum obtained by the thin film method is as shown in Fig. 1 and is the same as the standard product.

(6) The ¹³ C-NMR spectrum measured by dissolving in heavy water is as shown in Fig. 2 and the same as the standard product.

(7) Viscosity measurement method (TCLaurent et al., Bioch
im.Biophys.Acta, 42,476-485,1960), 200-
It was 3 million.

Examples of the hyaluronic acid salt include sodium salt, potassium salt, lithium salt, magnesium salt, calcium salt, lysine salt, ammonium salt, triethanolamine salt, propanolamine salt and the like.

The lactic acid bacterium culture solution used in the present invention is a conventional method, that is, lactic acid fermentation is carried out by inoculating lactic acid bacteria to a culture medium containing animal milk such as milk as a main component, and whey is fractionated from the obtained culture. It is manufactured by

Examples of lactic acid bacteria include Lactobacillus acidophilus, Bulgaricus, casei, Streptococcus
Thermofils etc. can be used. The animal milk used as the culture medium may be any of human milk, cow's milk, goat's milk, etc., and may be skim milk or reduced milk (including skim milk powder) of these animal milks. Of these, skim milk or reduced skim milk is particularly preferable because it can be easily treated after lactic acid fermentation. In addition to animal milk, it is desirable to add glucose, sucrose or the like, which is effective in promoting the growth of lactic acid bacteria, to the culture medium. The culture conditions do not have to be special, but the standard conditions are as follows:
After sterilizing by heating for about 15 minutes (or 110 ° C. for 90 minutes), lactic acid bacteria are inoculated and cultured at 37 ° C. for 2 to 3 days.

Whey is separated from the obtained culture by filtration or centrifugation.

The whey thus obtained, that is, the lactic acid bacterium culture solution can be used as it is, but since it has a unique odor, in the present invention, the whey is further reduced in pressure as in JP-A-58-192811. It is preferable to use the one in which a part of the whey is evaporated and heated until the aroma component in the whey is substantially removed. Further, the lactic acid bacterium culture solution may be concentrated and used.

In the cosmetics of the present invention, the amount of hyaluronic acid or a salt thereof added is 0.005 to 3% by weight (hereinafter simply referred to as%), particularly 0.005%.
~ 2.0% is preferable. The blending amount of the lactic acid bacterium culture solution is usually 0, though it varies depending on the concentration of the culture solution and the form of the cosmetic.
1 to 90% is preferable.

In addition to the above essential components, the cosmetic of the present invention appropriately contains various oils, surfactants, preservatives, viscosity modifiers, medicinal agents, other humectants, fragrances, alcohols, water, etc. which are usually blended with cosmetics. By doing so, for example, various creams, milky lotions, lotions, essences, packs, hair rinses, hair treatments, shampoos, lipsticks, foundations, hair restorers and the like can be formed.

The above-mentioned oil agents include liquid paraffin, petrolatum wax, paraffin wax, squalane, beeswax, higher alcohols, fatty acids, etc .; as surfactants, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyoxyethylene glycerin. Fatty acid ester, polyoxyethylene hydrogenated castor oil, polyoxyethylene sorbitol fatty acid ester and the like; alcohol as ethanol and the like; viscosity modifiers such as carboxymethyl cellulose, hydroxyethyl cellulose, polyvinyl alcohol, carboxy vinyl polymer, xanthan gum and the like; other Wetting agents include sorbitol, glycerin, 1,3-butylene glycol, sodium pyrrolidonecarboxylate, sodium lactate, polyethylene Parahydroxybenzoate alkyl ester as a preservative, sodium benzoate, potassium sorbate and the like; recall such vitamins as medicinal agents, anti-inflammatory agents, disinfectants, and the like. Of these optional ingredients, the addition of 1,3-butylene glycol further increases the effect of the present invention. The content of 1,3-butylene glycol is preferably 0.05 to 20%, more preferably 1 to 10%.

[Operation and effect of the invention]

The cosmetics of the present invention have a higher moisturizing effect than conventional cosmetics containing hyaluronic acid or lactic acid bacterium culture solution respectively, and have a feeling of use, for example, spread on the skin, moist feeling, and smoothness to skin (softness). And the like) are extremely good.

〔Example〕

Next, the present invention will be described in detail with reference to Reference Examples and Examples.

Reference Example 1 Streptococcus zooepidemicus, which exhibits β-hemolytic property, produces hyaluronidase, and produces hyaluronic acid, collected from bovine nasal mucosa, was cultured at 37 ° C. for 10 hours in Todd Hewitt Broth medium (manufactured by Difco). ,
The bacterial cells in the logarithmic growth phase were collected by centrifugation, and aseptically washed twice with 0.05 M Tris-maleic acid buffer (pH 6.0) while repeatedly centrifuging at low temperature, and then 1 × 10 ⁸ / ml
Suspend in the same buffer to obtain the bacterial concentration of
The mixture was added to 00 μg / ml and shaken at 37 ° C. for 30 minutes.
Subsequently, the cells under low temperature were washed twice with 0.05 M Tris-maleic acid buffer (pH 6.0), inoculated into Todd Hewitt broth medium and cultured at 37 ° C. for 18 hours. This culture solution was diluted with sterile physiological saline to 1 × 10 ³ / ml, and 0.1 ml of the diluted solution was added to blood (rabbit defibrinated blood) agar (20 ml), and after sprinkling culture, hemolysis was performed. Villages not shown were collected. The acquisition frequency of this mutant strain was about 4 × 10 ⁻⁶ . Next, this non-hemolytic strain was cultivated in Todd Hewitt Broth medium at 37 ° C. in the same manner as above, the bacterial cells in the logarithmic growth phase were collected, and 0.05 M Tris-maleic acid buffer solution (pH 6.0) was used. After washing, the plate was shaken in the same buffer containing 200 μg / ml of NTG at 37 ° C. for 20 minutes. Subsequently, the cells were washed under the low temperature with the same buffer solution, and then inoculated into an ultrafiltration medium (a high-molecular fraction removed from Tod Hewitt broth medium with Amicon ultrafiltration membrane YM-10). Then, the cells were cultured at 37 ° C for 18 hours. This culture solution was diluted with sterile physiological saline to 1-5 × 10 ² / ml, and 0.1 ml thereof was diluted with sodium hyaluronate (molecular weight: about 630,000, S
(manufactured by igma) Coated on the ultrafiltration medium agar (high-purity agar) containing 0.1% and cultured in a moisture chamber at 37 ° C. for 20-40 hours, and the bacteria in the grown colonies are replicated by the replica method. A hyaluronidase non-producing mutant Streptococcus zooepidemicus YIT203 that collects and sprays 10% cetylpyridinium chloride aqueous solution on agar to form a cloudy village around about 500,000 strains.
Got 0.

The method for distinguishing the hyaluronidase-producing and non-producing bacteria is the Erika-Balke et al., Zbl.Bakt.Hy method.
gA, 259,194-200,1985).

Reference Example 2 (batch culture) glucose 6%, polypeptone (manufactured by Daigo Nutrition Chemicals) 1.5
%, Baker's yeast extract (manufactured by Oriental Yeast Co., Ltd.) 0.5
%, Potassium secondary phosphate 0.2%, magnesium sulfate heptahydrate 0.1
%, Calcium chloride 0.05%, ADEKA NOL LG-109 (antifoaming agent made by Asahi Denka Kogyo) 0.001% of the medium (pH 7.0) 10
5 jar fermenter, sterilized, inoculated with 1% of pre-cultured Streptococcus zooepidemicus YIT2030, and continuously aerated at 37 ° C for 39 hours while continuously adjusting the culture pH to 7 with 6N-sodium hydroxide aqueous solution. Cultured.

Glucose was separately sterilized and added all at once at the start of culture. With the progress of the culture, hyaluronic acid accumulated and the viscosity of the culture solution reached nearly 8000 centipoise at 29 hours of culture, and almost no fluidity was reached.After 39 hours of culture, the culture was stopped when the glucose in the culture solution reached zero. finished.

Since the harvested culture broth is not fluid, it was diluted with water to a viscosity of 100 centipoise or less. Next, the pH of this solution was adjusted to 4 or less with trichloroacetic acid, and a hollow microfilter module (PW-103 Asahi Kasei)
Then, the bacterial cells and insoluble components were removed, and a low-molecular substance in the solution was removed by pouring water into the inner solution of the filtration through a hollow fiber ultrafiltration membrane (manufactured by HIP30-43 Amicon). Then, this solution was dried by a freeze-drying method to obtain 6.7 g of hyaluronic acid per culture solution. The molecular weight of the obtained hyaluronic acid was about 2.16 million (viscosity measuring method).

Reference Example 3 (continuous culture) 5 mediums having the same composition as in Reference Example 2 except that the glucose concentration was 2.5% were placed in 10 jar fermenters,
After sterilization (separate sterilization for glucose), inoculate 1% of pre-cultured Streptococcus zooepidemicus YIT2030, 6N
-While adjusting the culture pH to 7 with an aqueous solution of sodium hydroxide, the culture was performed with aeration and stirring at 37 ° C for 15 hours. After that, a medium having the same composition as that of Reference Example 2 was continuously added at a dilution rate of 0.3 (hr ⁻¹ ) except that the glucose concentration was adjusted to 2%.
Aeration-stirring continuous culture was carried out at ℃ and pH7 for 1 week. The culture solution that had flowed out of the culture tank was collected at regular intervals, and reference example 2
Hyaluronic acid (molecular weight of about 2.16 million [viscosity measuring method]) was extracted and purified in the same manner as in. As a result, the yield of hyaluronic acid with respect to sugar was 15%, and its productivity was 0.9 g // hr.
21.6 g / hyaluronic acid could be obtained.

Example 1 A lotion having the composition shown in Tables 1 and 3 was produced and the quality retention effect when applied to human skin was evaluated by the impedance method of Tagami et al. [Tagami et al, J. Invest. Dermatol., 75,500- 507,
1980] improved method for measuring skin permittivity [Karl Mosler,
Sonderdruck aus Parfmerie und kosmetik64,375-37
9, 1983] was applied and tested.

Test method After washing the inner part of the upper arm of a healthy male adult (27 years old) with soap, 4 x 4 cm marks were made on each of the left and right 3 parts, for a total of 6 parts.
After about 30 minutes, a skin surface water content measuring device (SKICOS3, Amic Group, probe diameter 16 mm) was used to measure the skin surface dielectric constant of each site before applying the skin lotion three times, and the average value was obtained. Immediately after that, the test lotion was applied to each part (16 cm ² ) of 30 μm.
It was applied and spread well with a finger. Then, the skin surface dielectric constant of each site was measured three times after application, and each average value was obtained. From the skin permittivity, the above-mentioned reference [Karl Mosler, Sonder
druck aus Parfmerie und Kosmetik64,375 ~ 379,198
According to 3], the water content on the skin surface was calculated. In addition,
In order to reduce the site difference and the error as much as possible, the lotion application site was changed, and each sample was measured at 6 sites, and the average value was adopted. The change in water content on the skin surface was expressed over time, with 0 being set before application of the sample. The relative humidity during the measurement period is
The temperature was 27 ± 7% and the room temperature was 26 ± 2 ° C.

Test Results The test results of the lotion of Table 1 are shown in Table 2, and the test results of the lotion of Table 3 are shown in Table 4. Further, these results, that is, the average value of the skin surface water content 10, 20, 30, 45, 60 minutes after the application of the lotion were determined and shown in FIGS. 3 and 4.

In Tables 1 and 2, low molecular weight hyaluronic acid has a molecular weight of about
780,000 (manufactured by Teikoku Organ Pharmaceutical Co., Ltd.) was used, high molecular hyaluronic acid was prepared according to Reference Example 2, and lactic acid bacterium culture liquid was whey containing aroma components removed [0.016 g
(Dry matter amount) / ml] (the same in the following examples). The numerical values in the table show% by weight.

From these results, the lotion of the present invention had a superior moisturizing effect as compared to the lotion containing hyaluronic acid or lactic acid bacterium culture solution alone. Further, the lotion of the present invention containing 1,3-butylene glycol added thereto had a further excellent moisturizing effect.

Example 2 A lotion having the composition shown in Table 5 was produced, and its use feeling was evaluated. In addition, the numerical value in Table 5 shows weight%.

Evaluation method and results: A lot of the lotions shown in Table 5 were applied to the backs of the hands of 10 panelists (5 males and 5 females), respectively, and spread on the skin, creases,
A questionnaire format was used to evaluate stickiness, richness, smoothness, and moistness.

As a result, the product 5 of the present invention showed an excellent feeling of use in all items as compared with the comparative product 8, and the product 6 of the present invention showed a superior feeling of use as compared with the comparative product 9. A statistically significant difference was found between these feelings of use, especially in terms of rich feeling, smooth feeling, and smooth feeling.

Example 3 An emulsion having the composition shown in Table 6 was produced, and its feeling of use was evaluated. The numerical values in Table 6 show% by weight.

Evaluation method and result: As a result of evaluation by the same method as in Example 2, the product 7 of the present invention
Showed an excellent feeling of use as compared with Comparative Products 10 and 11. A statistically significant difference was found between these feelings of use, especially in moist feeling and rich feeling.

Example 4 A cream having the composition shown in Table 7 was produced and its feeling of use was evaluated. In addition, the numerical value of Table 7 shows weight%.

Evaluation method and result: As a result of evaluation by the same method as in Example 2, the product 8 of the present invention
Showed a better feeling of use than Comparative Products 12 and 13. A statistically significant difference was found between these feelings of use, particularly in moist feeling and rich feeling.

Example 5 A lotion having the following composition was produced.

Composition: ethanol 10.0 (wt%) 1,3-butylene glycol 2.0 sodium hyaluronate (molecular weight 2.16 million) 0.2 polyoxyethylene hydrogenated castor oil (50E.0.) 0.
05 Methyl paraoxybenzoate 0.1 Perfume 0.1 Lactic acid bacterium culture solution Total amount of 100 Manufacturing method: was dispersed in, and was added to, and was sufficiently stirred to obtain a lotion.

Example 6 An emulsion having the following composition was produced.

Composition: Stearic acid 2.0 (wt%) Liquid paraffin 6.0 Squalane 2.0 Sorbitan monostearate 1.5 Polyoxyethylene sobitan monostearate (20
EO) 2.0 Butyl paraoxybenzoate 0.05 Na hyaluronate (Molecular weight 2.16 million) 0.2 1,3-Butylene glycol 3.0 Methyl paraoxybenzoate 0.1 Perfume 0.15 Amount of lactic acid bacterium culture solution to 100 Total production method: Add and, and , To obtain a dispersion. Then, this was added to ~ at 80 ° C to emulsify, then added and cooled to room temperature to obtain an emulsion.

Example 7 A cream having the following composition was produced.

Composition: Liquid paraffin 23.0 (% by weight) Vaseline 7.0 Cetanol 1.0 Stearic acid 2.0 Beeswax 2.0 Sorbitan monostearate 3.5 Polyoxyethylene sorbitan monostearate (20E.O.) 2.5 Butyl paraoxybenzoate 0.05 Na faluronate (Molecular weight 2.16 million) 0.2 1,3-Butylene glycol 3.0 Methyl paraoxybenzoate 0.1 Perfume 0.15 Lactic acid culture solution Amount that makes 100 as a whole Manufacturing method: and are added and dispersed to obtain a dispersion. This was then added to ~ at 80 ° C to emulsify, then add and cool to room temperature to give a cream.

[Brief description of drawings]

FIG. 1 and FIG. 2 are drawings showing the infrared absorption spectrum and the NMR spectrum of the polymer hyaluronic acid obtained in Reference Example 2, respectively. FIG. 3 and FIG. 4 are drawings showing average values of changes in skin surface water content up to 60 minutes after application of the lotion of Example 1.

Front page continuation (72) Inventor Koji Miyazaki 1-1-19 Higashi-Shimbashi, Minato-ku, Tokyo Yakult Honsha Co., Ltd. (72) Inventor Hiroshi Endo 1-1-19 Higashi-Shimbashi, Minato-ku, Tokyo Stock Association Company Yakult Head Office

Claims (3)

[Claims] 1. A cosmetic comprising hyaluronic acid or a salt thereof, and a lactic acid bacterium culture solution. 2. The cosmetic according to claim 1, wherein hyaluronic acid or a salt thereof has a molecular weight of 2,000,000 or more. 3. The cosmetic according to claim 1 or 2, which further contains 1,3-butylene glycol.