JPH0757180B2 - Method for storing VA mycorrhizal fungus - Google Patents
Method for storing VA mycorrhizal fungusInfo
- Publication number
- JPH0757180B2 JPH0757180B2 JP2317095A JP31709590A JPH0757180B2 JP H0757180 B2 JPH0757180 B2 JP H0757180B2 JP 2317095 A JP2317095 A JP 2317095A JP 31709590 A JP31709590 A JP 31709590A JP H0757180 B2 JPH0757180 B2 JP H0757180B2
- Authority
- JP
- Japan
- Prior art keywords
- spores
- carrier
- mycorrhizal
- germination rate
- zeolite
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241000233866 Fungi Species 0.000 title claims description 19
- 238000000034 method Methods 0.000 title claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 229910021536 Zeolite Inorganic materials 0.000 claims description 13
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims description 13
- 239000010457 zeolite Substances 0.000 claims description 13
- 239000002245 particle Substances 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 4
- 230000035784 germination Effects 0.000 description 22
- 239000002689 soil Substances 0.000 description 14
- 241000196324 Embryophyta Species 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 4
- 238000004321 preservation Methods 0.000 description 3
- 239000004576 sand Substances 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000006866 deterioration Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000010348 incorporation Methods 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 239000010455 vermiculite Substances 0.000 description 2
- 229910052902 vermiculite Inorganic materials 0.000 description 2
- 235000019354 vermiculite Nutrition 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 206010016807 Fluid retention Diseases 0.000 description 1
- 241000235502 Gigaspora margarita Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 239000012773 agricultural material Substances 0.000 description 1
- VDQQXEISLMTGAB-UHFFFAOYSA-N chloramine T Chemical compound [Na+].CC1=CC=C(S(=O)(=O)[N-]Cl)C=C1 VDQQXEISLMTGAB-UHFFFAOYSA-N 0.000 description 1
- 239000003245 coal Substances 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000000875 corresponding effect Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000003415 peat Substances 0.000 description 1
- 239000010451 perlite Substances 0.000 description 1
- 235000019362 perlite Nutrition 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000008262 pumice Substances 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Cultivation Of Plants (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
【発明の詳細な説明】 （産業上の利用分野） 本発明は、VA菌根菌の胞子を保存する方法に関するもの
である。TECHNICAL FIELD The present invention relates to a method for preserving spores of VA mycorrhizal fungi.
VA菌根菌は植物と共生させることにより植物の成育を旺
盛にさせ、土壌病害の発病を抑制軽減させることができ
るものである。VA mycorrhizal fungi can enhance the growth of plants by coexisting with plants, and can suppress and reduce the onset of soil diseases.
（従来の技術） VA菌根菌はリンやミネラル等の微量成分を土壌から吸収
して宿主植物に供給することから生物肥料としての役割
が注目され、作物の成育促進、病害抑制などに有効であ
ることから、新しい農業資材として有望視されている。(Prior art) Since VA mycorrhizal fungi absorbs trace elements such as phosphorus and minerals from the soil and supply them to host plants, their role as biological fertilizers is drawing attention, and they are effective in promoting the growth of crops and controlling disease. Therefore, it is considered as a promising new agricultural material.
かかるVA菌根菌の保存については一般には次のような方
法が用いられている。（１）宿根性植物の根にVA菌根菌
を共生させた状態（菌根）で植物を栽培して保存した
り、（２）VA菌根菌が共生した植物の栽培終了後の跡地
土壌を冷蔵庫（０℃〜10℃）で保管したり、あるいは
（３）跡地土壌より分離したVA菌根菌の胞子の表面を殺
菌し、０℃〜10℃に保たれた殺菌水中で保管している。The following method is generally used for the preservation of such VA mycorrhizal fungi. (1) Cultivate and store the plant in a state (mycorrhizal) in which VA mycorrhizal fungi coexist with the roots of the root-rooting plant, or (2) Soil after the cultivation of the plant in which the VA mycorrhizal fungus cohabits In a refrigerator (0 ° C to 10 ° C), or (3) sterilize the surface of VA mycorrhizal spores separated from the former soil and store it in sterile water kept at 0 ° C to 10 ° C. There is.
（発明が解決しょうとする問題点） 従来の方法では、植物を栽培して保存するためには広大
な施設を必要とし、しかも植物栽培が長期に亘る場合は
栽培中に雑菌による汚染の恐れがあること。跡地土壌を
そのまま保存するためには低温で保管する必要がありエ
ネルギーコストが高いこと。また、胞子を分離して表面
殺菌する方法は操作が煩雑であり、完全に殺菌すること
が困難なため保管中に雑菌による汚染の恐れがある。そ
のため、定期的に殺菌を繰り返す必要があり、しかも低
温で保管しなければならないのでエネルギーコストが高
い。そのため以上に述べた方法では、少量のVA菌根菌の
保存には適しているが、大量のVA菌根菌を保存する方法
としては不向きである。(Problems to be Solved by the Invention) In the conventional method, a vast facility is required to grow and store the plant, and when the plant is grown for a long period of time, there is a risk of contamination by various bacteria during the cultivation. To be. It is necessary to store it at low temperature in order to preserve the soil on the site and the energy cost is high. Further, the method of separating the spores and sterilizing the surface is complicated in operation, and it is difficult to completely sterilize the spores, so that there is a risk of contamination by various bacteria during storage. Therefore, it is necessary to repeat sterilization at regular intervals, and moreover, it is necessary to store it at a low temperature, resulting in high energy cost. Therefore, the method described above is suitable for storing a small amount of VA mycorrhizal fungi, but is not suitable as a method for storing a large amount of VA mycorrhizal fungi.
（問題点を解決するための手段） 本発明は上記の諸問題を解決するためになされたもの
で、VA菌根菌を保存する方法において、その胞子の担体
としてゼオライト混合物を用い、かつ担体の水分量を調
節することにより常温下でのVA菌根菌の胞子を長期間安
定的に保存することを可能としたものである。(Means for Solving Problems) The present invention has been made to solve the above-mentioned problems, and in a method for preserving VA mycorrhizal fungi, a zeolite mixture is used as a carrier for spores of By adjusting the water content, it is possible to stably store the spores of VA mycorrhizal fungi at room temperature for a long period of time.
以下本発明の方法を詳細に説明する。Hereinafter, the method of the present invention will be described in detail.
VA菌根菌の収集は、一般的な方法として行われている胞
子を含有した土壌を目開き2mmの篩に通し異物や大きな
土壌粒子を除去する。ついで、ウエット・シーヴィング
法等の常法を用いて胞子を分離し、保存用担体と混合し
密閉容器に充填するものである。The VA mycorrhizal fungus is generally collected by passing spore-containing soil through a 2 mm sieve to remove foreign matters and large soil particles. Then, the spores are separated by a conventional method such as the wet seving method, mixed with a storage carrier and filled in a closed container.
保存用担体としては、赤土、火山灰土、沖積土、砂、軽
石、パーライト、粉炭、ピートモスおよびバーミキュラ
イト等の園芸用培土とゼオライトとの混合物からなり、
ゼオライトを２〜50％（容量比）、好ましくは５〜30％
含有している担体を使用するものである。使用するゼオ
ライトは特に限定するものではなく、通常は粒径5mm以
下のものが好適に用いられる。ここで、ゼオライトの配
合がもたらす効果は明らかではないが、保存時担体の保
水に関与するものと考えられ、その配合量が２％以下で
はそれ相応の効果が乏しく、また50％以上では発芽に長
時間を要するので好ましくない。As a carrier for preservation, red soil, volcanic ash soil, alluvial soil, sand, pumice, perlite, pulverized coal, consisting of a mixture of horticultural soil such as peat moss and vermiculite and zeolite,
Zeolite 2-50% (volume ratio), preferably 5-30%
The carrier containing is used. Zeolite to be used is not particularly limited, and normally, one having a particle size of 5 mm or less is preferably used. Here, although the effect brought about by the incorporation of zeolite is not clear, it is considered to be involved in the water retention of the carrier during storage, and if the amount of incorporation is 2% or less, the corresponding effect is poor, and if it is 50% or more, germination does not occur. It is not preferable because it takes a long time.
なお、保存用担体とVA菌根菌の胞子を混合する前に、予
め保存用担体の含有水分を10〜30％、好ましくは20％付
近に調節しておくことが長期保存に好適である。Before mixing the spores of VA mycorrhizal with the carrier for storage, it is suitable for long-term storage to adjust the water content of the carrier for storage to 10 to 30%, preferably about 20%.
水分量が、10％以下では保存時の乾燥が影響し胞子の劣
化が起こり発芽率が低下する。また、30％以上になると
保存中に胞子が発芽するため好ましくない。If the water content is less than 10%, the spores will deteriorate due to drying during storage and the germination rate will decrease. Further, if it exceeds 30%, spores will germinate during storage, which is not preferable.
なお、担体に対するVA菌根菌胞子の混合量は特に規制は
ないが、担体1gr当たり５〜1000個程度である。The amount of VA mycorrhizal spores mixed with the carrier is not particularly limited, but is about 5 to 1000 per 1 gr of the carrier.
以下、実施例および比較例により本発明を説明するが、
これらによつて限定されるものではない。Hereinafter, the present invention will be described with reference to Examples and Comparative Examples.
It is not limited by these.
実施例１ 大豆を栽培した跡地土壌よりウエット・シーヴイング法
でVA菌根菌（Gigaspora Margarita）の胞子を分離し
た。ついで分離した胞子の発芽率を調べるため、胞子を
２％クロラミンＴ水溶液、および200ppmストレプトマイ
シン水溶液に各10分間浸漬し表面殺菌を行った。Example 1 Spores of VA mycorrhizal fungi (Gigaspora Margarita) were isolated from the soil where soybeans had been cultivated by the wet-sieving method. Then, in order to investigate the germination rate of the separated spores, the spores were immersed in a 2% chloramine T aqueous solution and a 200 ppm streptomycin aqueous solution for 10 minutes each for surface sterilization.
しかるのち、滅菌水で水洗後、寒天（デイフコ社製バク
トアガー）の平板上に殺菌済の胞子を12粒ずつのせ、25
℃インキュベターで培養した。Then, after washing with sterilized water, put 12 sterilized spores on each plate of agar (Dafco's Bactoagar), 25
The cells were cultured in an incubator at ℃.
培養１週間後、２週間後および３週間後の胞子の発芽率
を第１表に示す。胞子の状態は良好で、２週間で約80％
の胞子が発芽した。Table 1 shows the germination rates of spores after 1 week, 2 weeks and 3 weeks of culture. Spore condition is good, about 80% in 2 weeks
Spores germinated.
実施例２ 実施例１と同様に大豆を栽培した跡地より分離したVA菌
根菌の胞子を保存するため保存用担体と混合し、密閉容
器に充填し５℃の恒温器中で２ヵ月保管した。 Example 2 Similar to Example 1, spores of VA mycorrhizal fungi isolated from the site where soybeans were cultivated were mixed with a storage carrier for storage, filled in a closed container and stored in a thermostat at 5 ° C. for 2 months. .
保存用担体としては、粒径2mm以下の赤土に同じく2mm以
下のゼオライト（日東粉化工業（株））を０％、10％、
20％、50％（容量比）で混合し、含有水分を７％、15
％、20％、30％、45％となるように調整したものを用い
た。なお、VA菌根菌胞子は担体1gr当たり100個の割合で
混合したものを用い恒温器中で２ヵ月保管した後、密閉
容器中の保存用担体から胞子を分離し、実施例１に準じ
た方法で胞子の発芽率を調べた。第２表に培養２週間後
の胞子の発芽率を示す。As a carrier for preservation, 0%, 10% of red soil having a particle size of 2 mm or less and zeolite of 2 mm or less (Nitto Koka Kogyo Co., Ltd.),
Mix at 20% and 50% (volume ratio) to reduce the water content to 7% and 15
%, 20%, 30%, and 45% were used. The VA mycorrhizal spores were mixed at a ratio of 100 per 1 gr of the carrier, stored for 2 months in an incubator, and then the spores were separated from the carrier for storage in a hermetically sealed container. The germination rate of spores was examined by the method. Table 2 shows the germination rate of spores after 2 weeks of culture.
第２表からも判るように、低温下（５℃）での保存では
水分７％のときは胞子の劣化が認められたが、15％以上
では胞子の状態は良好であり発芽率も安定している。ま
た、ゼオライトの含有量が10％〜20％のものは発芽率も
優れており、低温保存であれば特に問題はない。 As can be seen from Table 2, deterioration of spores was observed when stored at low temperature (5 ° C) when the water content was 7%, but when the water content was 15% or more, the spore condition was good and the germination rate was stable. ing. Further, a zeolite having a zeolite content of 10% to 20% has an excellent germination rate, and there is no particular problem as long as it is stored at a low temperature.
実施例３ 実施例２と同様な方法で調整したVA菌根菌の胞子入り担
体を密閉容器に充填し、25℃の恒温器中で２ヶ月間保管
し、実施例１に準じた方法で胞子の発芽率を調べた。第
３表に培養２週間後の発芽率を示す。Example 3 A carrier containing spores of VA mycorrhizal fungi prepared in the same manner as in Example 2 was filled in a closed container, stored in a thermostat at 25 ° C. for 2 months, and spores were prepared in the same manner as in Example 1. Was examined for germination rate. Table 3 shows the germination rate after 2 weeks of culture.
第３表からもわかるように、含有水分７％のときは大半
の胞子が劣化していた。また、15％では一部の胞子に劣
化が認められたが発芽は安定していた。20％では胞子の
状態は最も良好であり、発芽率も安定していた。また、
30％以上では保管中に発芽がおこり保存には不適当であ
った。 As can be seen from Table 3, most of the spores were deteriorated when the water content was 7%. At 15%, some spores showed deterioration, but germination was stable. At 20%, the spore condition was the best and the germination rate was stable. Also,
When it was more than 30%, germination occurred during storage and it was unsuitable for storage.
また、ゼオライトの添加により胞子の表面は汚れが少な
くなり好ましいが、含有量が50％になると発芽率がやや
低下する。Further, the addition of zeolite is preferable because the surface of spores is less contaminated, but when the content is 50%, the germination rate is slightly lowered.
実施例４ 実施例２と同様な方法で調整したVA菌根菌の胞子を担体
（水分含有量７％、20％、45％、ゼオライト含有量20
％）と共に密閉容器に充填し、５℃および25℃の恒温器
中で８ヵ月間保管した。保管中定期的に胞子をサンプリ
ングし、実施例２に準じた方法で発芽率を調べた。第４
表および第５表に各温度における培養２週間後の発芽率
を示す。Example 4 The spores of VA mycorrhizal fungi prepared in the same manner as in Example 2 were used as a carrier (water content 7%, 20%, 45%, zeolite content 20).
%) In a closed container and stored in an incubator at 5 ° C. and 25 ° C. for 8 months. Spores were sampled periodically during storage and the germination rate was examined by the method according to Example 2. Fourth
Tables and Table 5 show the germination rate after 2 weeks of culture at each temperature.
第４表および第５表より５℃で保管した場合含有水分量
７％以外は８ヵ月後も胞子の状態が良好で発芽率も安定
していた。一方、25℃で保管した場合、含有水分が大略
20％の場合は胞子の状態は良好で且つ、発芽率も安定し
ており、５℃で保管した場合と同様の発芽率をしめして
いることが判つた。 From Tables 4 and 5, when stored at 5 ° C, the spores were in good condition and the germination rate was stable even after 8 months except that the water content was 7%. On the other hand, when stored at 25 ° C, the water content is
At 20%, the spores were in good condition and the germination rate was stable, showing that the germination rate was similar to that when stored at 5 ° C.
比較例１および２ 実施例２と同様な方法で調整したVA菌根菌の胞子を、粒
径2mm以下の赤土にバーミキュライト20％（容量比）混
合したもの（比較例１）および赤土に川砂20％（容量
比）混合したもの（比較例２）を含有水分が20％となる
ように調整し密閉容器に充填したのち、25℃の恒温器中
で８ヵ月間保管した。保管中に定期的に胞子をサンプリ
ングし、実施例１に準じた方法て発芽率を調べた。Comparative Examples 1 and 2 The spores of VA mycorrhizal fungi prepared in the same manner as in Example 2 were mixed with 20% vermiculite (volume ratio) of red soil having a particle size of 2 mm or less (Comparative Example 1) and red sand with river sand 20 % (Volume ratio) mixed product (Comparative Example 2) was adjusted to have a water content of 20%, filled in a closed container, and then stored in a thermostat at 25 ° C. for 8 months. Spores were periodically sampled during storage, and the germination rate was examined by the method according to Example 1.
第６表に培養２週間後の発芽率を示す。Table 6 shows the germination rate after 2 weeks of culture.
第６表から判るようにゼオライトを含有しない担体は、
これを含有する担体と比べ発芽率に可成りの差異が認め
られる。 As can be seen from Table 6, the carrier containing no zeolite is
A considerable difference in germination rate is recognized as compared with the carrier containing this.
（発明の効果） 本発明の方法によれば、簡便な方法でVA菌根菌胞子の常
温下における長期保存が可能である。(Effects of the Invention) According to the method of the present invention, VA mycorrhizal spores can be stored at room temperature for a long time by a simple method.
Claims (2)
担体として水分を10％〜30％含有させたゼオライト混合
物を用いることを特徴とするVA菌根菌の保存方法。1. A method for preserving VA mycorrhizal fungi, which comprises using a zeolite mixture containing 10% to 30% of water as a carrier for preserving spores of VA mycorrhizal fungus.
〜50％（容量比）混合する請求項１に記載の保存方法。2. Zeolite having a particle size of 5 mm or less is 2% of the carrier.
The storage method according to claim 1, wherein -50% (volume ratio) is mixed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2317095A JPH0757180B2 (en) | 1990-11-21 | 1990-11-21 | Method for storing VA mycorrhizal fungus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2317095A JPH0757180B2 (en) | 1990-11-21 | 1990-11-21 | Method for storing VA mycorrhizal fungus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH04187081A JPH04187081A (en) | 1992-07-03 |
| JPH0757180B2 true JPH0757180B2 (en) | 1995-06-21 |
Family
ID=18084384
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2317095A Expired - Fee Related JPH0757180B2 (en) | 1990-11-21 | 1990-11-21 | Method for storing VA mycorrhizal fungus |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0757180B2 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06189745A (en) * | 1992-12-25 | 1994-07-12 | Central Glass Co Ltd | Microbial material |
| WO1994016055A1 (en) * | 1993-01-07 | 1994-07-21 | Idemitsu Kosan Company Limited | Method of preserving spore of va mycorrhizal fungus |
| CN108315261A (en) * | 2018-04-13 | 2018-07-24 | 四川省农业科学院土壤肥料研究所 | A kind of edible mushroom storage medium and preparation method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03128988A (en) * | 1989-07-28 | 1991-05-31 | Tochigi Pref Gov | Microbial material capable of controlling soil disease and its manufacture |
-
1990
- 1990-11-21 JP JP2317095A patent/JPH0757180B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH04187081A (en) | 1992-07-03 |
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