JPH0759482B2 - Disinfectant - Google Patents

Description

TECHNICAL FIELD The present invention relates to a germicidal disinfectant using alkyl dimethyl benzyl ammonium or its salt containing about 80% by weight or more of dodecyl dimethyl benzyl ammonium or its salt.

BACKGROUND ART Alkyl dimethyl benzyl ammonium, for example, its chloride is called benzalkonium chloride (hereinafter benzalkonium chloride is sometimes abbreviated as BZK) or reverse soap, and it is a colorless and odorless water-soluble germicidal disinfection. It is widely used as an agent for medical purposes.

By the way, according to the 10th revised Japanese Pharmacopoeia, the side chain alkyl group of BZK in the BZK solution is C ₈ H ₁₇ , C ₁₀ H ₂₁ , C ₁₂ H ₂₅ , C ₁₄ H ₂₉ , C ₁₆ H ₃₃ ,
It is a mixture of C ₁₈ H ₃₇ and is said to consist mainly of C ₁₂ H ₂₅ and C ₁₄ H ₂₉ , and the ratio of the both is not specified. On the other hand, for various samples of commercially available BZK liquid, the content of BZK having each side chain is analyzed by a high performance liquid chromatography method [RC Meyer: Journal of Pharmaceutical Sciences (RCMeyer: J. Pharm.
Sci ..) 69, C ₁₂ as measured by 1148-1150,1980] H ₂₅ B
_{ZK 59-63%, C 14 H 29} BZK 29-34%, was C ₁₆ H ₃₃ BZK 6~8%.

As a general property of sterilizing agents, organic substances, for example, organic substances such as blood are mixed to reduce the sterilizing power. BZK is no exception to this rule, and it has been reported that the bactericidal activity is reduced to 1/2 to 1/4 when 10% high-concentration horse serum is present in the BZK solution [Heinemann P. G: Journal of. American Pharmaceutical Association (He
ineman, PG:.. J.Amer.Pharm, Assoc) 26, 711,1937].

In the case of a bactericidal disinfectant, unlike antibiotics, bactericidal power is exerted in a short time, so that emergence of resistant bacteria is not easy. Especially BZ
In the case of K, the time required for sterilization is extremely short, so that the appearance of resistant bacteria is hardly observed compared to other bactericides. However, in the case of products currently based on commercial BZK,
If the manufacturing process is inadequate, rarely
There is a possibility that microorganisms such as caligenes) and the genus Achromobacter may grow in the solution and be contaminated (Japanese Journal of Bacteriology, 15 , 1271, 1960).

Problems to be Solved by the Invention Although BZK is a sterilizing and disinfecting agent that is widely used at present, conventional compositions have further points to be improved as seen in the above findings.

One of them is that conventional compositions have a low bactericidal activity due to the inclusion of organic substances, so they are often used for medical purposes and sterilization of fingers, and are often used for organic purposes such as proteins. This is a problem in practical use as a bactericide because the bactericidal power decreases. Further, in the conventional composition, microorganisms such as Alcaligenes spp. And Achromobacter spp. May sometimes grow, and it is extremely practically possible to improve so as to show more effective bactericidal activity against a wider range of microorganisms than ever. It's important.

Means for Solving the Problems In view of the situation as described above, the present inventors have focused on that commercially available BZK has various side chain alkyl groups, and as a result of various studies, dodecyl dimethyl benzyl ammonium or By containing a certain amount or more of that salt, conventional BZ
It was found that the drawbacks of the K composition product can be eliminated (Japanese Patent Application No. 59-197072). The inventors of the present invention have made further studies, and by adding an appropriate amount of ethyl alcohol, isopropyl alcohol, sodium ethylenediaminetetraacetate or alkyldiaminoethylglycine hydrochloride together with the above-mentioned germicidal disinfectant, a further germicidal effect can be obtained. The present invention has been completed and further studies have been made to complete the present invention.

That is, the present invention provides (1) alkyl dimethyl benzyl ammonium or a salt thereof containing about 80% by weight or more of dodecyl dimethyl benzyl ammonium or a salt thereof (hereinafter, may be simply referred to as "the present ADBAC agent").
And a lower alcohol, a chelating agent, and one or more of amphoteric or nonionic surfactants. In this ADBAC agent, dodecyl dimethyl benzyl ammonium or its salt is a conventional method. The one obtained in
Especially, dodecyl dimethyl benzyl ammonium chloride can be advantageously used. The characteristic of this agent is that the content of dodecyl dimethyl benzylammonium or its salt as alkyl dimethyl benzylammonium or its salt is about 80% by weight or more, but it is more preferable to contain about 83% by weight or more. The effect is exhibited, and the upper limit content is not particularly limited, but is generally up to 95% by weight. As alkyl dimethyl benzyl ammonium other than dodecyl body or its salt, the side chain alkyl group is C ₈ H ₁₇ , C ₁₀ C ₂₁ , C ₁₄ H ₂₉ , C ₁₆ H ₃₃ or C
₁₈ H ₃₇ , and these may contain one kind or two or more kinds.

Next, the lower alcohol generally has 1 to 3 carbon atoms.
Those of ethyl alcohol and isopropyl alcohol are particularly advantageously used. As the chelating agent, an alkaline one is generally preferable, for example, sodium ethylenediaminetetraacetate or a polymerized phosphate (eg,
Sodium polyphosphate, potassium polyphosphate) and the like. Examples of amphoteric or nonionic surfactants include alkyl diaminoethylglycine hydrochloride (eg, dodecyl aminoethyl glycine hydrochloride, tetradecyl aminoethyl glycine hydrochloride), glycerin fatty acid ester (eg, glycerin Monolaurate, glycerin monocaprate) and the like.

Next, the amount ratio of the present ADBAC agent and each of the above-mentioned combined components in the sterilizing agent of the present invention is, for example, 100 parts by weight of the present ADBAC agent, lower alcohols are about 100 to 1,000,000 parts by weight, and preferably about 1 part by weight. 800 to 800,000 parts by weight, more preferably about 3.000 to 30,000 parts by weight when disinfecting fingers, instruments, etc., chelating agent about 0.02 to 1000 parts by weight, preferably about 0.2 to 500 parts by weight,
Further, when disinfecting equipment, indoors, etc., more preferably about 10 to 20
0 parts by weight, and about 0.2% amphoteric or nonionic surfactant.
˜1000 parts by weight, preferably about 0.5 to 200 parts by weight, and more preferably about 10 to 100 parts by weight when disinfecting fingers and the like.

The dosage form of this germicidal disinfectant depends on the shape of each combined ingredient,
It is prepared as a liquid agent or the like, and a known method can be used for the preparation method itself.

When using the present germicidal disinfectant, a formulation containing the present ADBAC agent and each combination component in the above-mentioned quantitative ratio is used as the concentration of the present ADBAC agent of about 0.01 to 5%, preferably about 0.02 to 3%, and further preferably Is diluted with an appropriate solvent, such as water, if necessary so as to be about 0.05 to 2% (weight / volume) and brought into contact with an object to be sterilized. Generally, lower alcohols are about 7
~ 90%, preferably about 15-80%, more preferably 15-30
% (Volume / volume), the chelating agent is about 0.001 to 0.1%, preferably about 0.005 to 0.1%, more preferably 0.05 to 0.1%.
(Weight / volume), and the amphoteric or nonionic surfactant is used at a concentration of about 0.01-0.1%, preferably about 0.02-0.1%, more preferably about 0.05-0.1% (weight / volume). .

This sterilization method generally has a pH of about 6.5-8.5, preferably 7-8.5.
It is carried out in 8.5, but depending on the type of the object of sterilization, when it is applied to medical equipment / facility, factory, etc., the pH is raised to about 9-11, preferably 10-11. Is preferable in terms of effect. As the pH adjuster in this case, an appropriate alkali agent can be used, and examples thereof include alkali hydroxide (eg, sodium hydroxide, potassium hydroxide), carbonate carbonate (eg, sodium carbonate, potassium carbonate, sodium hydrogen carbonate, (Potassium hydrogen carbonate) and the like.

In addition, since a bactericidal effect can be improved by imparting a cleaning action during sterilization and disinfection, the sterilization and disinfection effect can be further promoted by using a chemical having a cleaning or foaming ability in combination. Polymerized phosphates or surfactants can also be used together for such purpose, and in this case, these agents are generally contained in an amount of about 0.1 to 30% by weight.
It is preferable to use them together at a concentration of. Specific examples of the surfactant at this time include polyoxyethylene / polyoxypropylene / glycol ether, polyoxyethylene / nonylphenol / ether, and polyoxyethylene /
Octyl phenyl ether, coconut oil fatty acid amide / propyl betaine, coconut oil fatty acid diethanolamide, higher alcohol polyethylene glycol type nonionic surfactant and nonionic such as N-cocoyl-L-arginine ethyl ester-DL-pyrrolidone carboxylic acid And a surface active agent. Addition of one or more of these surfactants will be effective in disinfecting as well as preventing hand roughness. More specifically, about 10 to 30% of coconut oil fatty acid amide propyl betaine, or about 10 to 30% of polyoxyethylene polyoxypropylene glycol ether and about 3 to 10% of coconut oil fatty acid diethanolamide, and about 10 to 30% of ethanol. Addition increases the effectiveness of the disinfecting power. Furthermore, when hand disinfection with a germicidal disinfectant is frequently performed, the usefulness is improved by adding a skin protective agent such as a film-forming agent or a moisturizer to prevent rough hands. As skin protectant, lanolin, polyol,
Glycerin tri-2-ethylhexylate or the like can be added to the preparation in an amount of about 0.1 to 5% by weight and used. Further, the usefulness is improved by adding 0.01 to 2% by weight of sodium nitrite, which is well known as a rust preventive, for disinfecting equipment.

As a method for contacting the object to be sterilized and disinfected, a conventionally known method can be adopted, for example, dipping, spraying, sprinkling,
It may be carried out by washing or the like. For sterilization of fingers and the like, contaminated bacteria on the fingers can be sterilized by taking 3 to 10 ml of the above solution on the palm and thoroughly rubbing the fingers. The contact time is selected in consideration of the sterilization target or the concentration of the disinfectant used, but is usually about 10 seconds or more, preferably 1 minute or more.

The disinfectant can be widely applied to various uses for the purpose of eliminating microbial contamination. For example, medical treatment of affected areas of human bodies and animals or various medical instruments and facilities, disinfection of fingers,
Barber / beauty salon, public facilities such as schools, houses, vehicles, tableware,
It can be used for sterilizing and disinfecting food manufacturing factories or their manufacturing facilities. As shown in the experimental examples described below, this agent exhibits sufficient sterilizing and disinfecting power even when mixed with organic substances. Therefore, it can be preferably applied when the disinfectant solution is frequently used for medical purposes, or by disinfecting a finger or the like which is significantly contaminated with organic substances such as proteins. A preferred embodiment of this sterilization method is as follows, for example. Book ADBA
About 0.1 to 0.5% (weight / volume) of agent C and about 0.05 to 0.1% (weight / volume) of amphoteric or nonionic surfactant (eg, alkyl, diaminoethylglycine hydrochloride or / and glycerin fatty acid ester) Aqueous solution containing
A nurse or the like is preferably used as a sterilizing agent for hand washing before surgery. In the case of general hand sterilization, about 0.1 to 0.5 (weight / volume) of this ADBAC agent and about 15 to 30% (volume / volume) of lower alcohols (eg, ethyl alcohol or / and isopropyl alcohol) Or / and an aqueous solution containing about 0.05 to 0.1% (weight / volume) of an amphoteric or nonionic surfactant (eg, alkyl diaminoethylglycine hydrochloride or / and glycerin fatty acid ester) is palmed for about 10 seconds or more, It is possible to sterilize and disinfect without causing skin irritation by washing with rice for preferably about 30 to 60 seconds or more. In the case of medical equipment (eg, scissors, scalpel, etc.), about 0.05 to 0.2% (weight / volume) of this ADBAC agent and about 15 to 30 of lower alcohols (eg, ethyl alcohol or / and isopropyl alcohol) % (Volume / volume) or / and chelating agent (eg sodium ethylenediaminetetraacetate or / and polymerized phosphate)
The purpose of sterilization is generally achieved by immersing in an aqueous solution containing about 0.05 to 0.1% (weight / weight) of about 10 minutes or more. In addition, this AD is used for sterilization of indoors or vehicles.
An aqueous solution containing about 0.05 to 0.2% (weight / volume) of the BAC agent and about 0.05 to 0.1 (weight / weight) of the chelating agent (eg sodium ethylenediamitetraacetate or / and polymerized phosphate) is sprayed by a conventional method. This can be preferably carried out.

EXAMPLES Hereinafter, the present invention will be described more specifically with reference to experimental examples and examples.

In addition, "95% C₁₂BZK is an alkyl
Side chain is C₁₂H_{twenty five}95% of things, C₁₄H₂₉3%, C₁₆H₃₃of
BZK containing 1% of those containing 1% of other alkyl side chains
See you again, "83% C₁₂"BZK" means that the alkyl side chain is C₁₂H_{twenty five}
Of 83%, C₁₄H₂₉Of 13%, C₁₆H₃₃2% of that
BZK containing 1% of other alkyl side chains
It Commercial benzalkonium chloride used as a control
(Product name: Osvan ) Is C₁₂H_{twenty five}BZK about 60%, C₁₄H₂₉B
ZK about 30%, C₁₆H₃₃About 8% of BZK and about 1% of other BZK
Is included.

Experimental Example 1 As a test bacterium, Achromobacter guttatis A-39 and
And Alcaligenes faecalis 572 were used. That's it
1 platinum loop of each of these test bacteria was added to SCD medium (Daigo Nutrition Chemistry KK)
Inoculate the medium diluted to 50/50 (hereinafter abbreviated as 1/50 SCD) to 30
Cultivated at ℃ for 3 days. Commercially available benzalkonium chloride (Osba
The ), 95% C₁₂BZK and 83% C₁₂About BZK
The bactericidal power was measured by the following method.

That is, 1/50 SCD solutions alone were prepared into 1/50 SCD solutions containing 7%, 15% or 30% ethyl alcohol, respectively, to prepare solutions of the above bactericide at respective concentrations of 50 μg / ml to 10 mg / ml. Each of these test solutions was inoculated so that the viable cell count of each test bacterium in the culture was 10 ⁶ CFU / ml, and contacted at 25 ° C for 1 minute or 10 minutes. Immediately after the contact time, each of these solutions
Collect 500μ, 10ml of SCDLP medium (Daigo Nutrition Chemistry KK)
Moved to. For each of these cultures, the number of viable cells was measured on SCDLP agar plates and incubated at 35 ° C for 72 hours, and the presence or absence of growth of the test cells was observed. did.

The results of this experiment are shown in Table 1 (1) and (2). As is clear from these results, it was confirmed that 95% C ₁₂ BZK and 83% C ₁₂ BZK had extremely high bactericidal effect when used in combination with ethyl alcohol, as compared with commercial benzalkonium chloride.

Experimental Example 2 The hand disinfection effect by the glove juice method was examined. That is, the method of the sacred tree (Teruo sacred tree: Surgical practice 25 volumes, 2nd
No., pp. 256-261, 1983), the number of hand bacteria before sterilization (baseline value) was measured for 5 subjects, and then commercially available benzalkonium chloride, 95 % C ₁₂ BZK, 83
The disinfecting effect of 50% ethyl alcohol aqueous solution containing 0.1% of each% C ₁₂ BZK and 50% ethyl alcohol alone aqueous solution as a control was examined. Take 5 ml of these liquids on your palm, rub them over your fingers, and massage until dry. Repeat this operation 3 times. For the disinfected fingers, the number of bacteria was measured according to the above method. The sterilization rate was defined as the value obtained by subtracting 100 from the percentage value of the number of bacteria detected after disinfection divided by the baseline value. The results are shown in Table 2.

The sterilization rate shows the average value of 5 measurements, and when used in combination with ethyl alcohol, it is 95% higher than that of commercially available benzalkonium chloride.
It was shown that the sterilization rate was synergistically enhanced by the combined use with% C ₁₂ BZK or 83% C ₁₂ BZK.

Experimental Example 3 1/50 containing 1/50 SCD solution and 15% or 30% isopropyl alcohol using the same test strain and BZK as in Experimental Example 1
The bactericidal activity in the SCD solution was examined. The method is the same as in Experimental Example 1.

As can be seen from the results shown in Table 3, 95% C ₁₂ BZK or
It was found that 83% C ₁₂ BZK synergistically enhances the bactericidal activity as compared with commercial benzalkonium chloride when 15% or 30% isopropyl alcohol coexists.

Experimental Example 4 One platinum loop was taken from each slope culture of Pseudomonas cepacia TN1943 and Serratia marcescens 82-2-52 and inoculated on SCD medium (Daigo Nutrition Chemistry KK).
Incubated at ℃ for 20 hours. Commercially available benzalkonium chloride, 95% C ₁₂ BZK, 83% C ₁₂ BZK containing ethylene /
The bactericidal activity was measured for those to which diaminetetraacetic acid / 4 sodium and alkyl diaminoethyl glycine hydrochloride were added. That is, the test solution at each concentration was inoculated with the culture solution at about 10 ⁵ CFU / ml. After contacting for 15 seconds and 5 minutes after inoculation, these liquids were diluted 20 times with SCDLP medium (Daigo Nutrition Co., Ltd. KK) to inactivate each fungicide. The viable cell count in these solutions was measured and the bactericidal activity of each test solution was examined. The results are shown in Table 4.

Example 1 95% ethyl alcohol and water were poured into a 50% (w / v) 50% aqueous solution of 95% C ₁₂ BZK to dilute it to obtain ethyl alcohol as 15%.
%, BZK as 2% w / v solution, 1% w / v solution, 0.5% w / v
Solution, 0.2% w / v solution and 0.1% w / v solution were prepared. After taking 5 ml of each preparation solution in the palm and thoroughly scrubbing 3 times, the sterilization rate was 50% or more.

Example 2 95% C ₁₂ BZK was diluted with 50% (w / v) aqueous solution by injecting 95% ethyl alcohol water to obtain 30% ethyl alcohol.
And BZK as 2% w / v solution, 1% w / v solution, 0.5% w / v
Solution, 0.2% w / v solution and 0.1% w / v solution were prepared. After taking 5 ml of each preparation solution in the palm and thoroughly scrubbing 3 times, the sterilization rate was 50% or more.

Example 3 95% ethyl alcohol and water were poured into a 50% (w / v) aqueous solution of 83% C ₁₂ BZK to dilute it to obtain 20% ethyl alcohol.
%, BZK as 2% w / v solution, 1% w / v solution, 0.5% w / v solution, 0.2% w / v
And 0.1% w / v solution were prepared. 5 ml of each preparation
The sterilization rate was 50% or more when the sterilization rate was examined after picking it up on the palm of the hand and thoroughly rubbing it 3 times.

Example 4 95% isopropyl alcohol and water were poured into a 50% (w / v) aqueous solution of 83% C ₁₂ BZK to dilute it, and 20% as isopropyl alcohol and 2% w / v solution as BZK, 1% respectively. w / v
Solution, 0.5% w / v solution, 0.2% w / v solution and 0.1% w / v solution were prepared. After taking 5 ml of each preparation solution in the palm and thoroughly scrubbing 3 times, the sterilization rate was 50% or more.

Example 5 95% ethyl alcohol and water were added to a 50% (w / v) aqueous solution of 95% C ₁₂ BZK to dilute it to obtain ethyl alcohol as 15%.
%, And further dissolve ethylenediaminetetraacetic acid.4 sodium to give sodium ethylenediaminetetraacetate of 0.
05%, BZK 1%, 0.5%, 0.2% and 0.1% w respectively
A / v solution was prepared. After taking 5 ml of each preparation solution in the palm and thoroughly scrubbing 3 times, the sterilization rate was 50% or more.

Example 6 95% isopropyl alcohol and water were poured into a 50% (w / v) aqueous solution of 83% C ₁₂ BZK to dilute it to 15% as isopropyl alcohol, and 30% alkyldiethylaminoethylglycine hydrochloride (trade name). : Tego 51: containing 18% of dodecyl aminoethylglycine hydrochloride and 12% of tetradecyl aminoethylglycine hydrochloride.),
Alkyldiethylaminoethylglycine hydrochloride as 0.
05%, BZK 1%, 0.5%, 0.2% and 0.1% w respectively
A / v solution was prepared. After taking 5 ml of each preparation solution in the palm and thoroughly scrubbing 3 times, the sterilization rate was 50% or more.

Example 7 Sodium ethylenediaminetetraacetate was dissolved in a 50% (w / v) aqueous solution of 83% C ₁₂ BZK to obtain 0.1% sodium ethylenediaminetetraacetate and 2% w / v as BZK respectively.
Solutions, 1% w / v solution, 0.5% w / v solution, 0.2% w / v solution and 0.1% w / v solution were prepared. Each preparation solution was sprayed indoors, and after 10 minutes, the sterilization rate was 90% or more.

Example 8 30% alkyldiethylaminoethylglycine hydrochloride was dissolved in a 50% (w / v) aqueous solution of 95% C ₁₂ BZK to obtain 0.05% alkyldiethylaminoethylglycine hydrochloride and 2% w / v solutions as BZK. , 1% w / v solution, 0.5% w / v solution, 0.2% w / v
Solution and 0.1% w / v solution were prepared. 5m of each preparation
It was 50% or more when the sterilization rate was examined after pickling on the palm and thoroughly rubbing 3 times.

Example 9 83% C ₁₂ BZK 50% (W / V) aqueous solution was diluted by adding tap water and sodium tripolyphosphate was dissolved to obtain 0.1% (W / V) sodium tripolyphosphate and BZK 1%, 0.5. %
Liquids containing 0.2% and 0.1% (W / V) were prepared. Each adjusting liquid 2 was put into a stainless steel container, and medical instruments such as scissors and a scalpel were immersed. The sterilization rate was 90% or more after 10 minutes of immersion.

Example 10 Injecting 90% isopropyl alcohol and water into a 50% (W / V) aqueous solution of 83% C ₁₂ BZK to dilute it to 15% (W / V) as isopropyl alcohol, and further add ethylenediaminetetraacetic acid. By dissolving 4 sodium, 0.05% (W / V) of ethylenediaminetetraacetic acid / 4 sodium and 1%, 0.5%, 0.2% (W / V) of BZK were prepared. Each of the adjustment liquids 2 was placed in a synthetic resin container, and medical instruments such as scissors and a scalpel were immersed. Sterilization rate after 90 minutes of immersion is 90%
That was all.

Example 11 Glycerin monolaurate was dissolved in a 50% (W / V) 50% aqueous solution of 95% C ₁₂ BZK to prepare a glycerin monolaurate of 0.1%.
005%, 2% W / V liquid as BZK, 1% W / V liquid, 0.5% W / V
Solution, 0.2% W / V solution and 0.1% W / V solution were prepared. 5 ml of each of the prepared liquids was picked up on the palm and thoroughly scrubbed 3 times, and the sterilization rate was 50% or more.

Example 12 A 50% (w / v) aqueous solution of 83% C ₁₂ BZK was diluted by injecting 95% ethyl alcohol and water to obtain 50% ethyl alcohol.
%, BZK as 2% w / v solution, 1% w / v solution, 0.5% w / v solution, 0.2% w / v
And 0.1% w / v solution were prepared. 5 ml of each preparation
The sterilization rate was 50% or more when the sterilization rate was examined after picking it up on the palm of the hand and thoroughly rubbing it 3 times.

Example 13 95% isopropyl alcohol water was poured into a 50% (w / v) aqueous solution of 83% C ₁₂ BZK to dilute it to 40% as isopropyl alcohol, and 30% alkyldiethylaminoethylglycine hydrochloride (trade name: Tego 51: Dodecyl-aminoethylglycine hydrochloride 18% and tetradecyl-aminoethylglycine hydrochloride 12% are dissolved) to give 0.02 as alkyldiethylaminoethylglycine hydrochloride.
%, BZK were prepared as 1%, 0.2% and 0.1% w / v solutions, respectively. After taking 5 ml of each preparation solution in the palm and thoroughly scrubbing 3 times, the sterilization rate was 50% or more.

Example 14 30% Alkyldiethylaminoethylglycine hydrochloride was dissolved in 50% (w / v) aqueous solution of 95% C ₁₂ BZK to prepare 0.1% of alkyldiethylaminoethylglycine hydrochloride and 2% w / v of BZK respectively. , 1% w / v solution, 0.5% w / v solution, 0.2% w / v
Solution and 0.1% w / v solution were prepared. 5m of each preparation
It was 50% or more when the sterilization rate was examined after pickling on the palm and thoroughly rubbing 3 times.

Example 15 250 g of the surfactant polyoxyethylene polyoxypropylene glycol ether was dissolved in 500 ml of sterilized tap water, and 50 g of coconut oil fatty acid diethanolamide was further dissolved therein. Add 83% C ₁₂ BZK 40% (W / V) solution to this solution.
3.75 ml, 25 ml, 50 ml or 125 ml was injected. Next, after adding 200 ml of 95% ethyl alcohol to each of these liquids,
Sterilized tap water was added to adjust the total amount to 1,000 ml, and 0.15%, 1%, 2% and 5% (W / V) solutions were prepared as total BZK, respectively. Approximately 5 ml of each preparation was placed on the palm of the hand, thoroughly washed with rubbing, and the sterilization rate was 95% or more.

Example 16 Surfactant 100 g of coconut oil fatty acid amide propyl betaine was dissolved in 500 ml of sterilized tap water, and further 100 g of polyoxyethylene polyoxypropylene glycol ether was dissolved therein. Add 83% C ₁₂ BZK 40% (W / V) aqueous solution to this solution.
5 ml, 25 ml, 50 ml or 125 ml was injected. Next, after adding 200 ml of 95% ethyl alcohol to each of these liquids, sterilized tap water was added to make the total amount 1,000 ml each, and the total BZK was 0.15%, 1%, 2% and 5% (W / V), respectively. A liquid was prepared. Approximately 5 ml of each prepared solution was found on the palm, and after thoroughly scrubbing, the sterilization rate was 95% or more.

EFFECTS OF THE INVENTION The present invention comprises an alkyl dimethyl benzyl ammonium or a salt thereof containing about 80% by weight or more of dodecyl dimethyl benzyl ammonium or a salt thereof, a lower alcohol, a chelating agent and an amphoteric or nonionic surfactant. It is characterized by using one kind or two or more kinds in combination. According to this combination, the lower alcohol, the chelating agent, or the amphoteric or nonionic surfactant is usually used in an amount such that the sterilizing and disinfecting effect is not significantly exhibited by itself, but the alkyl dimethyl benzyl ammonium or the salt thereof is used. The sterilizing and disinfecting power of is significantly enhanced. In addition, when the concentration is such that the disinfecting power can be recognized by using lower alcohols, chelating agents, or amphoteric or nonionic surfactants alone, synergistic effects can be obtained by using them together with the above alkyl dimethyl benzyl ammonium or a salt thereof. The sterilizing and disinfecting power is enhanced. Such combined effect is
Compared with the case of alkyl dimethyl benzyl ammonium having a conventional composition or a salt thereof, it is extremely remarkably observed.

As a result, the sterilization method of the present invention can be widely and effectively applied to various kinds of objects, and in particular, it is practically useful in that the sterilization and disinfection power is less likely to decrease even if organic substances are present in the objects. Is.

Claims (1)

[Claims] 1. An alkyl dimethyl benzyl ammonium or a salt thereof containing dodecyl dimethyl benzyl ammonium or a salt thereof in an amount of about 80% by weight or more, and a lower alcohol, a chelating agent and an amphoteric or nonionic surfactant. A sterilizing agent containing one or more species.