JPH0779681B2 - Yeast preparation with enhanced iodine content and method for producing the same - Google Patents

Description

Description: FIELD OF THE INVENTION The present invention relates to a yeast preparation having an enhanced iodine content, which allows iodine to be ingested easily and in a preferred form, and a method for producing the same.

BACKGROUND OF THE INVENTION Iodine is an essential element for living organisms, and its involvement in thyroid function has been clarified by previous studies. Further, this iodine-containing preparation is manufactured for the purpose of treating various diseases other than thyroid dysfunction.

In general, a drug for ingesting iodine has side effects such as gastrointestinal disorders, iodine rash, and iodine catarrh. For the problem of this side effect, a method of ingesting iodine as an organic compound such as iodine lecithin has been proposed and implemented. However, in order to take iodine in a large amount or on a daily basis, a material containing iodine in a more preferable condition from a medical or nutritional viewpoint is required.

SUMMARY OF THE INVENTION An object of the present invention is to provide a material capable of ingesting iodine easily and in a preferable form.

The present invention has iodine resistance such that it can be produced in a medium having a potassium iodide concentration of 1.0% or more, contains at least 1 μg of iodine per 100 g of dry weight, and is subjected to cell wall destruction treatment. The present invention provides a yeast preparation having enhanced iodine content, which comprises the above yeast in combination with an excipient or a carrier.

The yeast preparation has iodine resistance such that it can be produced in a medium having a potassium iodide concentration of 1.0% or more by stepwise increasing the iodine compound concentration in the medium used in the culture of the yeast group. It can be easily produced by simultaneously selecting a strain and culturing the strain with iodine ingestion; subjecting the yeast to cell wall disruption treatment; and formulating with an excipient or carrier.

[Advantages of the Invention] The iodine-containing yeast of the present invention can easily ingest most of this iodine compound into the living body by ingesting the iodine compound into the yeast, without causing the above-mentioned side effects. It has been converted to a state. Therefore, the iodine-containing yeast of the present invention is suitable as a material for ingesting iodine in large amounts or on a daily basis. Further, the iodine-containing yeast of the present invention does not impair the excellent nutritional properties of yeast cells, and is therefore suitable as a material for a nutritional supplement that is routinely ingested. Furthermore, for the purpose of treating a specific disease by iodine administration, it is preferable to use the above-mentioned yeast having been subjected to cell wall destruction treatment as an easy-to-drink formulation suitable for oral administration by using an excipient or a carrier. it can.

Further, the method for producing the iodine-containing yeast of the present invention has the advantage that the production of the iodine-containing yeast can be easily carried out because it is possible to simultaneously perform the iodine-intake strain selection and the iodine-intake culture of the strain. Have.

[Detailed Description of the Invention] In addition to the use of yeast for the production of fermented foods, yeast is used as a nutritional supplement or as a food supplement or feed, focusing on the excellent nutritional properties of yeast cells (especially protein and vitamin). There are uses. However, in such applications for directly ingesting yeast cells, almost no attempts have been made to positively enhance specific components of the cells.

According to the research conducted by the present inventor, iodine-resistant strains exist as natural mutant strains with a certain frequency in the yeast group that is usually used in the fermentation industry and the like. In addition, these iodine-resistant strains also have iodine uptake.
Therefore, the iodine uptake strain used in the practice of the present invention is easily selected from yeasts commonly used,
Can be used.

There is no particular limitation on the yeast strain that can be used in the present invention, and bacterial strains generally used in the fermentation industry can be used. However, considering the fact that it is easily available and the research is progressing, it is preferable to use yeast belonging to the genus Saccharomyces, particularly Saccharomyces Cerevisiae.

The iodine-containing yeast of the present invention is characterized by containing at least 1 μg of iodine per 100 g of dry weight (preferably at least 0.1 mg per 100 g of dry weight) as a result of analysis of the elemental composition of the microbial cells. When the above yeast is used as a material for a drug intended for treatment by active iodine administration, it is preferable that the yeast contains at least 10 mg of iodine per 100 g of dry weight. However, yeast containing 1 μg to 10 mg of iodine per 100 g of dry weight in the cells is also preferably used as a nutritional supplement for iodine deficiency or as a material for a drug intended for treatment by administration of a small amount of iodine. be able to.

Iodine uptake strains used in the practice of the present invention include:
As described above, a natural mutant strain can be selected from a yeast group in which the trait is relatively unfixed. That is, an iodine uptake strain can be selected by culturing the yeast group in a medium containing a high concentration of iodine compound. By using the strain of the iodine-ingesting strain obtained as described above, it is possible to ingest a high concentration of iodine into yeast cells. Further, it is also possible to form a strain having a further excellent iodine uptake property and other properties by artificial mutation using radiation or drugs.

Further, according to the method for producing a yeast of the present invention, by gradually increasing the iodine compound concentration in the medium used in the culture of the yeast group, it is possible to simultaneously perform the selection of the iodine uptake strain and the iodine uptake culture of the strain. it can. As a method of stepwise increasing the iodine concentration in the medium, a plurality of mediums having different iodine compound concentrations are prepared, and a method of sequentially transplanting yeast groups to a medium having a higher iodine compound concentration is a method of iodine-ingesting strains. Efficient in selection. Alternatively, a method of gradually increasing the iodine compound concentration by gradually adding the iodine compound to the liquid medium can be used.

The medium used in the selection and culture of the yeast, including the yeast production method of the present invention, is not particularly limited,
A synthetic medium or a complex medium as used in Examples described later may be used as long as it is a medium generally used for yeast culture. Further, since there are no particular restrictions on the culture conditions (pH, temperature, etc.), it is preferable to carry out the culture under the optimal conditions for the yeast to be used. Further, although it depends on the yeast species, it is generally preferable to carry out under aerobic conditions from the viewpoint of production efficiency.

In selecting and culturing the above yeast, it is preferable to use a metal salt of iodine as the iodine compound added to the medium. Examples of particularly preferred metal salts of iodine include:
Mention may be made of potassium iodide and sodium iodide.

The iodine concentration of the medium used in the method for producing yeast of the present invention is preferably about 0.001% at the start of culture when potassium iodide or sodium iodide is used. Further, the concentration of potassium iodide or sodium iodide in the medium at the end of the culture is determined according to the iodine content per dry weight finally required, but a relatively high content (dry weight 100 g For the purpose of producing bacterial cells (10 mg or more per cell), it is preferably 1.0% or more.

The time required for culturing the yeast is about 12 to about 24 hours for each medium having a different iodine concentration when aiming at the selection of iodine-ingesting strains, and the purpose is to ingest the iodine compound into the yeast. In this case (in the production method of the present invention, the final culture in the highest concentration medium) is preferably carried out for about 20 to about 24 hours.

The yeast of the present invention produced as described above can be used in various methods. Generally, when the main purpose is the oral ingestion of yeast cells, the cell walls that impede digestion and absorption are subjected to physical or chemical destruction treatment. Other methods for treating bacterial cells can be prepared in various forms depending on the purpose of ingesting iodine. For example, it can be carried out with reference to a known method for preparing a yeast preparation (such as a brewer's yeast preparation) and a method for treating cells for the purpose of food production (protein production). Particularly, for the purpose of medical administration of iodine, it is preferable to prepare the above-mentioned yeast which has been subjected to cell wall destruction treatment in the form of tablets or capsules together with an excipient or carrier. When preparing as described above, the yeast is generally dried and powdered. As described above, the thus-prepared formulation of the present invention having an enhanced iodine content is particularly excellent for oral administration.

Examples of the present invention will be shown below.

[Example 1] 0.001 of potassium iodide was added to a medium having the following composition.
1% (I), 0.01% (II), 0.05% (III) and 0.1%
(IV) The added one was created.

Yeast growth medium composition (per water) Glucose 10 g Peptone 5 g Powdered yeast extract 3 g Powdered malt extract 3 g The pH was adjusted to 5.6 using 1N hydrochloric acid.

A baker's yeast (Saccharomyces Cerevisiae) strain was cultivated in a medium (I) having the lowest potassium iodide concentration at 27 ° C. for 24 hours, then transferred to a medium (II), cultivated in the same manner, and sequentially. Transplantation and culture were repeated in the mediums (III) and (IV). In the last culture in (IV) medium, a strain having an iodine uptake capacity of 10 mg or more per 100 g of dry weight was obtained. This strain was continuously cultivated in the medium (IV) for 24 hours to allow sufficient intake of iodine. The analysis results of the obtained iodine-containing yeast are shown below.

Iodine 18.3 mg per 100 g dry weight (analysis result of Japan Food Analysis Center, by iodine titration method) The iodine-containing yeast according to the present invention was powdered and put into a capsule to prepare a yeast preparation.

When 1 to 3 g of the above yeast preparation was taken 1 to 3 times a day, no side effect was observed.

Continuation of front page (51) Int.Cl. ⁶ Identification code Office reference number FI technical display area C12R 1: 865)

Claims (11)

[Claims] 1. Having iodine resistance such that it can be produced in a medium having a potassium iodide concentration of 1.0% or more, and containing at least 1 μg of iodine per 100 g of dry weight,
Then, a yeast preparation having enhanced iodine content, which comprises yeast treated with cell wall destruction treatment together with an excipient or a carrier. 2. The yeast preparation according to claim 1, wherein the yeast has been dried and powdered. 3. The yeast preparation according to claim 1, which is for oral administration. 4. The yeast according to at least 100 g of dry weight.
The yeast preparation according to claim 1, which contains 0.1 mg of iodine. 5. The yeast according to at least 100 g of dry weight.
The yeast preparation according to claim 4, which contains 10 mg of iodine. 6. The yeast preparation according to claim 1, wherein the yeast is a yeast belonging to the genus Saccharomyces. 7. The yeast preparation according to claim 1, wherein the yeast is Saccharomyces cerevisiae. 8. A stepwise increase in the concentration of an iodine compound in a medium used for culturing a yeast group,
Selection of a strain having iodine resistance that can be produced in a medium having a potassium iodide concentration of 1.0% or more and cultivation of iodine uptake of the strain are simultaneously performed; yeast is subjected to cell wall destruction treatment; Characterized in that it is prepared using a carrier,
It has iodine resistance such that it can be produced in a medium with a potassium iodide concentration of 1.0% or more, and at least dry weight.
A method for producing a yeast preparation having an enhanced iodine content, which comprises 1 μg of iodine per 100 g and which has been subjected to cell wall disruption treatment together with an excipient or a carrier. 9. A method for increasing the iodine concentration in a medium in a stepwise manner by preparing a plurality of mediums having different iodine compound concentrations and transplanting yeast strains into a medium having a higher iodine compound concentration in order. The method for producing the yeast preparation according to claim 8. 10. The method for producing a yeast preparation according to claim 8, wherein the iodine compound is potassium iodide. 11. The method according to claim 10, wherein the potassium iodide concentration in the medium at the start of the culture is 0.001% or less, and the potassium iodide concentration in the medium at the end of the culture is 1.0% or more. A method for producing the yeast preparation described.