JPH0781999B2 - High performance liquid chromatograph for analysis of catecholamines - Google Patents
High performance liquid chromatograph for analysis of catecholaminesInfo
- Publication number
- JPH0781999B2 JPH0781999B2 JP61070044A JP7004486A JPH0781999B2 JP H0781999 B2 JPH0781999 B2 JP H0781999B2 JP 61070044 A JP61070044 A JP 61070044A JP 7004486 A JP7004486 A JP 7004486A JP H0781999 B2 JPH0781999 B2 JP H0781999B2
- Authority
- JP
- Japan
- Prior art keywords
- catecholamines
- analysis
- performance liquid
- liquid chromatograph
- high performance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Description
【発明の詳細な説明】 イ．産業上の利用分野 本発明はカテコールアミンの分析に適した高速液体クロ
マトグラフ装置に関する。Detailed Description of the Invention a. TECHNICAL FIELD The present invention relates to a high-performance liquid chromatographic apparatus suitable for analyzing catecholamines.
ロ．従来技術 生体試料に含まれているカテコールアミン類の分析は、
通常固定相側に逆相カラムを、移動相側にはリン酸バッ
ファ液にイオンペア剤や、アセトニトリルや、メタノー
ル等を試料毎に種々の割合で添加して調製したものを用
いて行われる。B. Prior art Analysis of catecholamines contained in biological samples
Usually, a reversed phase column is used on the stationary phase side, and a mobile phase side is prepared by adding an ion pair agent, acetonitrile, methanol or the like to a phosphate buffer solution at various ratios for each sample.
しかしながら、色々の薬剤を混合する必要があるため、
移動相液の調製に熟練を要するばかりでなく、第３図に
示したように、クロマトグラム上で試料中の尿酸やアス
コルビン酸の検出ピークP1、P2が目的成分のピークに重
なって測定ミスを引き起こすという問題があった。However, since it is necessary to mix various drugs,
Not only does it take skill to prepare the mobile phase solution, but as shown in Fig. 3, the peaks of detection of uric acid and ascorbic acid P 1 and P 2 in the sample on the chromatogram overlap with the peaks of the target component. There was the problem of causing mistakes.
ハ．目的 本発明はこのような問題に鑑みてなされたものであっ
て、その目的とするところは単純な組成の移動相液を使
用することができるとともに、誤差要因となる尿酸やア
スコルビン酸を早期に排出させて目的成分のピークを独
立させることができる新規なクロマトグラフ装置を提供
することにある。C. The present invention has been made in view of such a problem, the purpose is to be able to use a mobile phase liquid of a simple composition, uric acid and ascorbic acid as an error factor early It is an object of the present invention to provide a novel chromatographic apparatus capable of discharging and making the peak of a target component independent.
ニ．発明の概要 すなわち、本発明が特徴とするところは、強陽イオン交
換樹脂を固定相に、またリン酸系溶液を移動相に用いた
点にある。D. SUMMARY OF THE INVENTION That is, what is characteristic of the present invention is that a strong cation exchange resin is used as a stationary phase and a phosphoric acid solution is used as a mobile phase.
ホ．実施例 そこで以下に本発明の詳細を図示した実施例に基づいて
説明する。E. Embodiments Details of the present invention will be described below based on illustrated embodiments.
第１図は本発明の一実施例を示すものであって、図中符
号１は強陽イオン交換樹脂を充填剤とした分析カラム
で、一端が0.08規定の燐酸ナトリウム溶液を収容したキ
ャリア液槽２に試料インジェクタ３を介して連通し、ま
た他端が電気化学検出器４に接続されている。FIG. 1 shows an embodiment of the present invention. In the figure, reference numeral 1 is an analytical column having a strong cation exchange resin as a packing material, and a carrier liquid tank containing a sodium phosphate solution having 0.08N at one end. 2 through the sample injector 3, and the other end is connected to the electrochemical detector 4.
この電気化学検出器４の第１電極4aと第２電極4bには、
0.05V程度の電位差を維持するように0.15V以下の電位が
測定回路５から供給されている。なお、図中符号６、７
は、それぞれ試料インジェクタ３の上流側の管路に接続
したガードセル、及び送液ポンプをそれぞれ示す。In the first electrode 4a and the second electrode 4b of this electrochemical detector 4,
A potential of 0.15 V or less is supplied from the measuring circuit 5 so that the potential difference of about 0.05 V is maintained. In the figure, reference numerals 6 and 7
Indicates a guard cell and a liquid feed pump, which are connected to the pipes on the upstream side of the sample injector 3, respectively.
この実施例において、分析カラム１にキャリア液を流し
た状態で、除タンパク等の前処理を施した試料を試料イ
ンジェクタ３から注入すると、試料はキャリア液と共に
分析カラム１に運ばれ、ここにおいてイオン交換が行わ
れてアスコルビン酸や尿酸が早期に排出される一方、カ
テコールアミン類が各成分毎に分離される。In this embodiment, when the sample pretreated with deproteinization is injected from the sample injector 3 while the carrier liquid is flowing through the analysis column 1, the sample is carried to the analysis column 1 together with the carrier liquid, where the ions are While exchange is performed and ascorbic acid and uric acid are excreted early, catecholamines are separated into each component.
このようにして、分析カラム１から排出された成分が電
気化学検出器４に流入すると、各成分は第１電極4aと第
２電極4b間の電位差を受けて酸化還元を受ける。これに
より検出器４は、第２図に示したように尿酸やアスコル
ビン酸に起因するピークをソルベントピーク中に含んで
出力してしまった後、カテコールアミン類をＬ−ドー
パ、ノルエピネフィリン、エピネフィリン、デイハイド
ロオキシベンジイルアミン、及びドーバピンをそれぞれ
単峰性の独立したピークとして出力する。In this manner, when the components discharged from the analytical column 1 flow into the electrochemical detector 4, each component receives the potential difference between the first electrode 4a and the second electrode 4b and undergoes redox. As a result, the detector 4 outputs the catecholamines containing L-dopa, norepinephrine, and epinephrine after the peaks resulting from uric acid and ascorbic acid are included in the solvent peak as shown in FIG. , Dehydroxybenzodiylamine, and dovapine are output as unimodal independent peaks.
なお、この実施例においてはキャリア液としてリン酸ナ
トリムウ溶液を使用したが、リン酸アンモニウム（NH4
−H2PO4）の溶液を用いても同様の作用を奏する。Although sodium phosphate solution was used as the carrier liquid in this example, ammonium phosphate (NH 4
-H 2 PO 4 ) solution has the same effect.
ヘ．効果 以上、説明したように本発明によれば強陽イオン交換樹
脂を固定相に、リン酸系溶液を移動相に用いるととも
に、電極間電位差を0.05V程度に維持した電気化学検出
器で検出するようにしたので、検体中の尿酸やアスコル
ビン酸をソルベントピークに包含させてカテコールアミ
ン類をそれぞれ独立のピークとして検出することができ
るばかりでなく、キャリア液の組成を簡素化してランニ
ングコストの引き下げや調製作業の単純化を図ることが
できる。F. Effects As described above, according to the present invention, a strong cation exchange resin is used as a stationary phase, a phosphoric acid solution is used as a mobile phase, and detection is performed by an electrochemical detector that maintains an interelectrode potential difference of about 0.05V. As a result, not only can uric acid and ascorbic acid in the sample be included in the solvent peak to detect catecholamines as independent peaks, but also the composition of the carrier liquid can be simplified to reduce running costs and preparation. The work can be simplified.
第１図は本発明の一実施例を示す装置の構成図、第２図
は同上装置によるクロマトグラム図、及び第３図は従来
装置によるカテコールアミン類の分析結果を示すクロマ
トグラム図である。 １……分析カラム、２……キャリア液相 ３……電気化学検出器FIG. 1 is a block diagram of an apparatus showing an embodiment of the present invention, FIG. 2 is a chromatogram chart by the same apparatus, and FIG. 3 is a chromatogram chart showing the analysis results of catecholamines by a conventional apparatus. 1 ... Analytical column, 2 ... Carrier liquid phase 3 ... Electrochemical detector
Claims (1)
ム、移動相としてのリン酸系溶液を前記分析カラムに供
給する手段、及び前記分析用カラムの排出側に接続され
て電極間電位差が0.05V程度に維持された電気化学検出
手段からなるカテコールアミン類分析用高速液体クロマ
トグラフ装置。1. An analytical column filled with a strong cation exchange resin, a means for supplying a phosphoric acid-based solution as a mobile phase to the analytical column, and a potential difference between electrodes connected to the discharge side of the analytical column of 0.05. A high-performance liquid chromatograph for catecholamines analysis, which comprises an electrochemical detection means maintained at about V.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61070044A JPH0781999B2 (en) | 1986-03-27 | 1986-03-27 | High performance liquid chromatograph for analysis of catecholamines |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61070044A JPH0781999B2 (en) | 1986-03-27 | 1986-03-27 | High performance liquid chromatograph for analysis of catecholamines |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62225954A JPS62225954A (en) | 1987-10-03 |
| JPH0781999B2 true JPH0781999B2 (en) | 1995-09-06 |
Family
ID=13420181
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61070044A Expired - Fee Related JPH0781999B2 (en) | 1986-03-27 | 1986-03-27 | High performance liquid chromatograph for analysis of catecholamines |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0781999B2 (en) |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0675066B2 (en) * | 1984-05-02 | 1994-09-21 | 株式会社島津製作所 | Catecholamine detector |
-
1986
- 1986-03-27 JP JP61070044A patent/JPH0781999B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPS62225954A (en) | 1987-10-03 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |