JPH0789875B2 - Rotifer feed - Google Patents
Rotifer feedInfo
- Publication number
- JPH0789875B2 JPH0789875B2 JP63067962A JP6796288A JPH0789875B2 JP H0789875 B2 JPH0789875 B2 JP H0789875B2 JP 63067962 A JP63067962 A JP 63067962A JP 6796288 A JP6796288 A JP 6796288A JP H0789875 B2 JPH0789875 B2 JP H0789875B2
- Authority
- JP
- Japan
- Prior art keywords
- chlorella
- vitamin
- rotifer
- culture
- rotifers
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241000700141 Rotifera Species 0.000 title claims description 63
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims description 58
- FDJOLVPMNUYSCM-UVKKECPRSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2,7, Chemical compound [Co+3].N#[C-].C1([C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)[N-]\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O FDJOLVPMNUYSCM-UVKKECPRSA-L 0.000 claims description 36
- 239000000725 suspension Substances 0.000 claims description 8
- 238000004519 manufacturing process Methods 0.000 description 10
- 229930003231 vitamin Natural products 0.000 description 8
- 235000013343 vitamin Nutrition 0.000 description 8
- 239000011782 vitamin Substances 0.000 description 8
- 229940088594 vitamin Drugs 0.000 description 8
- 241000251468 Actinopterygii Species 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- 229930003270 Vitamin B Natural products 0.000 description 4
- 238000012258 culturing Methods 0.000 description 4
- 235000019156 vitamin B Nutrition 0.000 description 4
- 239000011720 vitamin B Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 235000005911 diet Nutrition 0.000 description 3
- 239000013505 freshwater Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000013535 sea water Substances 0.000 description 3
- 150000003722 vitamin derivatives Chemical class 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 230000037213 diet Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 235000019341 magnesium sulphate Nutrition 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- 241000224474 Nannochloropsis Species 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 238000004820 blood count Methods 0.000 description 1
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 235000003891 ferrous sulphate Nutrition 0.000 description 1
- 239000011790 ferrous sulphate Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 229910000150 monocalcium phosphate Inorganic materials 0.000 description 1
- 235000019691 monocalcium phosphate Nutrition 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 229910021654 trace metal Inorganic materials 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Feed For Specific Animals (AREA)
- Farming Of Fish And Shellfish (AREA)
- Fodder In General (AREA)
Description
【発明の詳細な説明】 〔産業上の利用分野〕 本発明は魚類の種苗生産に用いられるワムシを効率的に
安定して生産するためのワムシ用餌料に関するものであ
る。Description: TECHNICAL FIELD The present invention relates to a rotifer feedstuff for efficiently and stably producing a rotifer used for seed production of fish.
水産種苗の初期餌料としてワムシは最も重要な餌料であ
り、ナンノクロロプシス、酵母、淡水産濃縮クロレラ等
を用いたワムシの大量培養法の開発により、種苗生産の
飛躍的な増加が可能となった。しかしながら現在でもワ
ムシ培養においては、時としてワムシの増殖不良が起こ
り、そのため種苗生産に支障をきたすことがあるので、
ワムシのより安定な、効率の良い培養法の確立が望まれ
ている。Rotifers are the most important foods as an initial feed for aquatic seedlings, and the development of a large-scale cultivation method for rotifers using Nannochloropsis, yeast, freshwater concentrated chlorella, etc. has enabled a dramatic increase in seedling production. . However, even in the culture of rotifers, the rotifers sometimes grow poorly, which may hinder seed production.
It is desired to establish a more stable and efficient culture method for rotifers.
ワムシ培養の不安定性の一因として、ワムシ培養槽の細
菌相が変化して、ワムシに必要なビタミンB12を細菌が
供給できなくなることが示唆されており、ワムシ安定培
養のために細菌相解明の試みもなされているが、まだこ
れをコントロールすることは困難である。またワムシ培
養槽に直接ビタミンB12を添加する方法も考えられる
が、ワムシ培養槽100t当り１〜5gと大量のビタミンB12
が必要となるうえ、添加したビタミンが他の細菌等によ
って分解を受け、ワムシがビタミンを効率的に利用でき
ず、経済的でないので実用化には至っていない。As one of the causes of instability of rotifer culture, it has been suggested that the bacterial flora in the rotifer culture tank is changed and the bacteria cannot supply vitamin B 12 required for rotifer, and the bacterial flora is clarified for stable culture of rotifer. Have been attempted, but it is still difficult to control this. It is also possible to add vitamin B 12 directly to the rotifer culture tank, but a large amount of vitamin B 12 of 1 to 5 g per 100 t of the rotifer culture tank is possible.
In addition, the added vitamin is decomposed by other bacteria and the like, and the rotifer cannot efficiently use the vitamin, which is not economical and has not been put into practical use.
本発明の目的は、現在ワムシ餌料として広く使用されて
いる淡水産クロレラに、ワムシに必要なビタミンB12を
高濃度に含有させ、ワムシ餌料として供給することによ
り、効率の良い、安定したワムシ生産を可能にするワム
シ餌料を提供することである。An object of the present invention is to provide freshwater chlorella, which is widely used as a rotifer diet at present, with a high concentration of vitamin B 12 required for rotifers, and to supply it as a rotifer diet, thereby producing an efficient and stable rotifer production. It is to provide a rotifer feed that enables
本発明は、培地またはクロレラ懸濁液中に添加したビタ
ミンB12を吸収、蓄積したクロレラ藻体を含有すること
を特徴とするワムシ餌料である。The present invention is a rotifer feed containing chlorella algal cells that have absorbed and accumulated vitamin B 12 added to a medium or a chlorella suspension.
クロレラの培養時の培養液、または培養後のクロレラ懸
濁液に、ビタミンB12を添加してクロレラに吸収、蓄積
させることにより、ビタミンB12高含有クロレラを得る
ことができる。Vitamin B 12- rich chlorella can be obtained by adding vitamin B 12 to the culture solution for culturing chlorella or the chlorella suspension after culturing to allow the chlorella to absorb and accumulate.
クロレラは通常、窒素源として硝酸塩、アンモニウム
塩、尿素などを用い、その他燐酸一カリウム、硫酸マグ
ネシウム、硫酸第一鉄などの無機塩、および必要に応じ
てグルコース、酢酸、アミノ酸などの有機物を添加した
培養液を用い、明条件または暗条件にて、通気あるいは
撹拌して培養される。これらの培養液を調製する時、適
当量のビタミンB12を培養液中に加えてクロレラを培養
することにより、培養液に添加したビタミンB12は、培
養終了後にはほぼすべてがクロレラ中に吸収、蓄積さ
れ、非常に効率よくビタミンB12高含有クロレラを製造
することができる。このときのクロレラの培養条件は通
常の培養の場合と同様である。Chlorella usually uses nitrate, ammonium salt, urea, etc. as a nitrogen source, and other inorganic salts such as monopotassium phosphate, magnesium sulfate, ferrous sulfate, and, if necessary, organic substances such as glucose, acetic acid and amino acid are added. The culture is aerated and agitated under light or dark conditions. When preparing these culture solutions, by adding an appropriate amount of vitamin B 12 to the culture solution and culturing the chlorella, almost all of the vitamin B 12 added to the culture solution is absorbed in the chlorella after the culture is completed. It is possible to produce chlorella that is high in vitamin B 12 content, accumulated and very efficiently. The culture conditions for Chlorella at this time are the same as those for normal culture.
また一般的なクロレラの培養方法に従ってクロレラを培
養し、培養終了時のクロレラ懸濁液に適当量のビタミン
B12を添加して保持すると、約10時間程度で添加したビ
タミンB12を100％クロレラに吸収、蓄積させることがで
きる。この方法によれば、培養液を調製する時にビタミ
ンを添加する方法に比べ短時間で吸収、蓄積させること
ができ、滅菌などによるビタミンの分解もないことか
ら、さらに効率的なビタミンB12高含有クロレラの製造
が可能である。Also, cultivate chlorella according to the general chlorella culture method, and add an appropriate amount of vitamins to the chlorella suspension at the end of the culture.
When B 12 is added and held, 100% chlorella can absorb and accumulate the added vitamin B 12 in about 10 hours. According to this method, the absorption in a short time compared with the method of adding vitamins when preparing the culture solution, it is possible to accumulate, since there is no degradation of vitamins due sterilization, more efficient vitamin B 12 high content It is possible to manufacture chlorella.
クロレラのビタミンB12の含有量は、培養液またはクロ
レラ懸濁液中へのビタミンB12の添加量を加減すること
により、１〜1100μg/100gの範囲で、任意の濃度のもの
を製造することができる。その時の培養液またはクロレ
ラ懸濁液中へのビタミンB12の添加量は、その培養液に
よって生産されるクロレラの濃度またはクロレラ懸濁液
中のクロレラの濃度等によって異なる。たとえばクロレ
ラが7g/l生産される培養液または同濃度のクロレラ懸濁
液では、ビタミンB12の培養液またはクロレラ懸濁液中
への添加量は0.5〜200μg/lの範囲で、ビタミンB12含有
クロレラの製造が可能であり、種々の濃度のビタミンB
12を含有するクロレラを製造することができるが、特に
0.5〜100μg/lの範囲が効率的であり、200μg/lを超え
る添加では効率は低くなる。Regarding the content of vitamin B 12 in chlorella, the amount of vitamin B 12 added to the culture broth or chlorella suspension should be adjusted to produce an arbitrary concentration in the range of 1 to 1100 μg / 100 g. You can The amount of vitamin B 12 added to the culture solution or chlorella suspension at that time varies depending on the concentration of chlorella produced by the culture solution, the concentration of chlorella in the chlorella suspension, or the like. For example, in the chlorella suspension culture or the same concentration Chlorella is 7 g / l production, the amount of the culture medium or Chlorella suspension of vitamin B 12 in the range of 0.5~200μg / l, vitamin B 12 It is possible to produce chlorella containing vitamin B in various concentrations.
Chlorella containing 12 can be produced, but especially
The range of 0.5 to 100 μg / l is effective, and the addition of more than 200 μg / l results in low efficiency.
本発明では、このようにして高濃度にビタミンB12を吸
収、蓄積させたクロレラを製造し、このクロレラをワム
シ用餌料として利用する。In the present invention, chlorella in which vitamin B 12 is absorbed and accumulated at a high concentration in this manner is produced, and this chlorella is used as a rotifer feed.
ビタミンB12を最低20μg/100g、望ましくは100〜200μg
/l00g程度含有させたクロレラはワムシ生産用の餌料と
して利用することにより、従来工業的に生産されている
ワムシ餌料である酵母、淡水産濃縮クロレラに比べ、ワ
ムシの生育が非常に良くなり、したがってワムシの効率
的な安定生産が可能となる。この場合、ビタミンB12含
有クロレラは生あるいは乾燥物の使用が可能である。Vitamin B 12 at least 20 μg / 100 g, preferably 100-200 μg
By using chlorella containing about 100g / l as a feed for rotifer production, rotifers grow much better than yeast, which is a conventional industrially produced rotifer feed, and freshwater concentrated chlorella. It enables efficient and stable production of rotifers. In this case, the chlorella containing vitamin B 12 can be used raw or dried.
またワムシへの投餌方法は毎日、１回または数回適当量
のクロレラを与えるのが一般的であるが、少量の連続的
な投餌または他の餌料と混合して与えることも可能であ
る。The method for feeding rotifers is generally to give an appropriate amount of chlorella once or several times daily, but it is also possible to give a small amount of continuous feeding or mixing with other feeds. .
従来ワムシのビタミンB12は、ワムシ培養槽内に発生す
る細菌により供給されており、このような細菌を抑制す
ることは難しく、ワムシ培養の不安定の原因の一つであ
ったが、本発明の高濃度のビタミンB12を含有するクロ
レラをワムシに与えることにより、安定してビタミンB
12をワムシに供給することが可能となり、ワムシの増殖
が良くなるとともに、ワムシ培養の安定化が可能とな
る。Conventionally, rotifer vitamin B 12 is supplied by bacteria generated in the rotifer culture tank, and it is difficult to suppress such bacteria, which was one of the causes of instability of rotifer culture. By supplying rotifers with chlorella containing a high concentration of vitamin B 12 ,
It becomes possible to supply 12 to the rotifer, the growth of the rotifer is improved, and the rotifer culture can be stabilized.
この場合、クロレラは100％ワムシに取り込まれるた
め、クロレラに含有しているビタミンB12は非常に効率
的にワムシに利用され、ワムシ培養槽100tに対しクロレ
ラに含有させて添加するビタミンB12は0.02〜0.1gと非
常に少量で有効である。In this case, chlorella is taken up by 100% of the rotifer, so the vitamin B 12 contained in chlorella is used very efficiently in the rotifer, and the vitamin B 12 added to chlorella in 100 t of the rotifer culture tank is Effective in a very small amount of 0.02-0.1g.
また魚類に対しても、ビタミンB12は有効であり、欠乏
すると血球数、ヘモグロビン量が低下することが知られ
ている。従ってビタミンB12含有クロレラでワムシを培
養することにより、魚類に対しても優れた栄養価を有す
るワムシの生産が可能となる。Vitamin B 12 is also effective for fish, and it is known that deficiency reduces blood cell counts and hemoglobin levels. Therefore, by culturing rotifers with Vitamin B 12- containing Chlorella, it is possible to produce rotifers with excellent nutritional value for fish.
実施例１ 種々のクロレラ株の培養液にビタミンB12を添加して培
養を行い、クロレラによるビタミンB12の吸収、蓄積を
調べた。培養条件は25℃、３〜5kluxの条件で、グルコ
ース20g/l、尿素1.5g/l、リン酸一カルシウム1.2g/l、
硫酸マグネシウム0.6g/l、鉄EDTA 15mg/l、微量金属A5
溶液1ml/l、ビタミンB1240μg/lを含む培地を使用し、
３日間培養した。Example 1 Vitamin B 12 was added to culture solutions of various chlorella strains and cultured, and absorption and accumulation of vitamin B 12 by chlorella were examined. Culture conditions are 25 ° C, 3-5klux, glucose 20g / l, urea 1.5g / l, monocalcium phosphate 1.2g / l,
Magnesium sulfate 0.6g / l, iron EDTA 15mg / l, trace metal A5
Use a medium containing 1 ml / l solution, 40 μg / l vitamin B 12 ,
Cultured for 3 days.
こうして得られた種々の濃度のビタミンB12を含むクロ
レラをワムシに与えてその餌料価値を調べた。すなわち
人工海水ジャマリンＳにクロレラ1500万細胞/mlを懸濁
し、人工海水で良く洗ったＬ型ワムシを１個体/ml接種
して、25℃、弱光下にて培養を行い、ワムシ個体数の変
化を調べた。結果を第１図に培養日数とワムシ密度のグ
ラフとして示す。Chlorella containing various concentrations of vitamin B 12 thus obtained was fed to rotifers to examine their dietary value. That is, 15 million cells / ml of Chlorella was suspended in artificial seawater Jamarin S, and 1 L / type rotifer that had been thoroughly washed with artificial seawater was inoculated at 1 individual / ml and cultured at 25 ° C under low light to determine the number of rotifers. I examined the changes. The results are shown in Fig. 1 as a graph of culture days and rotifer density.
第１図の結果により、従来の方法で製造したビタミンB
12をほとんど含まないクロレラ（Ａ、Ｂ）で培養したワ
ムシは生育が非常に悪く、ビタミンB12含量4.3μg/100g
のものもワムシの生育は悪かった。Based on the results in Figure 1, vitamin B produced by the conventional method
Rotifers cultivated in Chlorella (A, B) containing almost no 12 have very poor growth and have a vitamin B 12 content of 4.3 μg / 100g.
The rotifer also had a bad growth.
一方ビタミンB12を22,45,180μg/100g以上含むクロレラ
（Ｃ、Ｄ、Ｅ）で培養したワムシの生育は良く、ワムシ
餌料として従来のクロレラより非常に優れていた。On the other hand, the rotifers cultivated with Chlorella (C, D, E) containing 22,45,180 μg / 100 g or more of vitamin B 12 grew well, and was far superior to the conventional chlorella as a rotifer feed.
実施例２ ビタミンB12100μg/100g含有するクロレラと、通常の方
法で培養したビタミンB12を含まないクロレラでワムシ
を培養し、その餌料価値を比較した。ワムシは15lの人
工海水中において、22〜24℃で通気条件で培養した。毎
日ワムシの個体数を測定し、ワムシ個体数に見あった量
のクロレラを投餌した。ビタミンを含むクロレラを与え
たワムシは４日目、５日目に卵率が高くなり、ワムシの
増殖もはるかに良かった。７日目にはワムシ密度が605/
mlに達したので、ワムシの大部分を収穫し、一部分で新
たに培養を開始した。And chlorella containing Example 2 Vitamin B 12 100 [mu] g / 100 g, were cultured rotifers Chlorella containing no vitamin B 12 was cultured in the usual way, and comparing the feed value. Rotifers were cultivated in 15 liters of artificial seawater at 22-24 ° C with aeration. The number of rotifers was measured every day, and chlorella was fed in an amount matching the number of rotifers. Rotifers fed with chlorella containing vitamins had higher egg rates on the 4th and 5th days, and the rotifers grew much better. On the 7th day the rotifer density is 605 /
Since the amount reached to ml, most of the rotifer was harvested and a new culture was started in part.
一方ビタミンB12を含まないクロレラで培養したワムシ
は、11日後のワムシ密度は552/mlに止まった。ビタミン
を含むクロレラを与えた区では11日間で30.7gのクロレ
ラを与えて1575万のワムシが生産できたのに対し、ビタ
ミンを含まない区では15.4gのクロレラしか与えること
ができず、804万のワムシ生産であった。On the other hand, rotifers cultured in Chlorella without vitamin B 12 had a rotifer density of only 552 / ml after 11 days. In the group that was given vitamin-containing chlorella, 30.7 g of chlorella could be given in 11 days to produce 15.75 million rotifers, whereas in the group that did not contain vitamins, only 15.4 g of chlorella could be given, which was 8.04 million. It was rotifer production.
結果を第２図に培養日数とワムシ密度および卵率のグラ
フとして示す。The results are shown in Fig. 2 as a graph of culture days, rotifer density and egg rate.
本発明によれば、ビタミンB12を吸収、蓄積したビタミ
ンB12高含有クロレラをワムシ餌料としたため、効率の
良い安定したワムシ生産が可能になり、魚類に対する栄
養価も高くなる。According to the present invention, the absorption of vitamin B 12, since the accumulated vitamin B 12 high content chlorella was rotifers feed allows for efficient stable rotifer production, nutritional value higher for fish.
第１図および第２図は実施例の結果を示すグラフであ
る。1 and 2 are graphs showing the results of the examples.
Claims (1)
タミンB12を吸収、蓄積したクロレラ藻体を含有するこ
とを特徴とするワムシ餌料。1. A rotifer feed containing chlorella algal cells that have absorbed and accumulated vitamin B 12 added to a medium or a chlorella suspension.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63067962A JPH0789875B2 (en) | 1988-03-22 | 1988-03-22 | Rotifer feed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63067962A JPH0789875B2 (en) | 1988-03-22 | 1988-03-22 | Rotifer feed |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH01240128A JPH01240128A (en) | 1989-09-25 |
| JPH0789875B2 true JPH0789875B2 (en) | 1995-10-04 |
Family
ID=13360099
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP63067962A Expired - Fee Related JPH0789875B2 (en) | 1988-03-22 | 1988-03-22 | Rotifer feed |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0789875B2 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003055330A1 (en) * | 2001-12-26 | 2003-07-10 | Kyushu Tlo Company, Limited | Feed for culturing animal planktons such as brachionus rotundiformis |
| KR100447940B1 (en) * | 2002-03-21 | 2004-09-10 | 학교법인 한양학원 | Development of profitable prey, green algae Coelastrum sp. |
| WO2010089864A1 (en) * | 2009-02-04 | 2010-08-12 | クロレラ工業株式会社 | Selenium-containing unicellular microalgae for animal plankton feeds and method of culturing selenium-containing animal planktons using the same |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5944020B2 (en) | 2006-12-22 | 2016-07-05 | キヤノン株式会社 | Electrophotographic image forming apparatus and electrophotographic photosensitive drum unit |
-
1988
- 1988-03-22 JP JP63067962A patent/JPH0789875B2/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5944020B2 (en) | 2006-12-22 | 2016-07-05 | キヤノン株式会社 | Electrophotographic image forming apparatus and electrophotographic photosensitive drum unit |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH01240128A (en) | 1989-09-25 |
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