JPH0820416B2 - Dye or pesticide analysis method - Google Patents
Dye or pesticide analysis methodInfo
- Publication number
- JPH0820416B2 JPH0820416B2 JP5222766A JP22276693A JPH0820416B2 JP H0820416 B2 JPH0820416 B2 JP H0820416B2 JP 5222766 A JP5222766 A JP 5222766A JP 22276693 A JP22276693 A JP 22276693A JP H0820416 B2 JPH0820416 B2 JP H0820416B2
- Authority
- JP
- Japan
- Prior art keywords
- dye
- pesticide
- organic solvent
- electrophoresis
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000000575 pesticide Substances 0.000 title claims description 9
- 238000004458 analytical method Methods 0.000 title description 13
- 238000000034 method Methods 0.000 claims description 19
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims description 12
- 238000001962 electrophoresis Methods 0.000 claims description 12
- 239000003960 organic solvent Substances 0.000 claims description 10
- 239000000126 substance Substances 0.000 claims description 8
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 6
- QGJOPFRUJISHPQ-UHFFFAOYSA-N Carbon disulfide Chemical compound S=C=S QGJOPFRUJISHPQ-UHFFFAOYSA-N 0.000 claims description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 6
- 238000005251 capillar electrophoresis Methods 0.000 claims description 5
- 239000000049 pigment Substances 0.000 claims description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 4
- 239000003905 agrochemical Substances 0.000 claims description 3
- 235000013305 food Nutrition 0.000 claims description 3
- 235000019441 ethanol Nutrition 0.000 claims description 2
- 239000000975 dye Substances 0.000 description 14
- 239000000523 sample Substances 0.000 description 10
- 238000000926 separation method Methods 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 238000001514 detection method Methods 0.000 description 6
- 150000002500 ions Chemical class 0.000 description 6
- 230000035945 sensitivity Effects 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 5
- 238000013508 migration Methods 0.000 description 4
- 230000005012 migration Effects 0.000 description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 125000000129 anionic group Chemical group 0.000 description 3
- 125000002091 cationic group Chemical group 0.000 description 3
- -1 chelate complex ion Chemical class 0.000 description 3
- 239000005350 fused silica glass Substances 0.000 description 3
- 239000013028 medium composition Substances 0.000 description 3
- PAYRUJLWNCNPSJ-UHFFFAOYSA-N Aniline Chemical compound NC1=CC=CC=C1 PAYRUJLWNCNPSJ-UHFFFAOYSA-N 0.000 description 2
- AFVFQIVMOAPDHO-UHFFFAOYSA-N Methanesulfonic acid Chemical compound CS(O)(=O)=O AFVFQIVMOAPDHO-UHFFFAOYSA-N 0.000 description 2
- MRQIXHXHHPWVIL-ISLYRVAYSA-N Sudan I Chemical compound OC1=CC=C2C=CC=CC2=C1\N=N\C1=CC=CC=C1 MRQIXHXHHPWVIL-ISLYRVAYSA-N 0.000 description 2
- BWLVSYUUKOQICP-FMQUCBEESA-N Yellow OB Chemical compound CC1=CC=CC=C1\N=N\C1=C(N)C=CC2=CC=CC=C12 BWLVSYUUKOQICP-FMQUCBEESA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 150000001768 cations Chemical class 0.000 description 2
- 230000006866 deterioration Effects 0.000 description 2
- 239000002917 insecticide Substances 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- CXKWCBBOMKCUKX-UHFFFAOYSA-M methylene blue Chemical compound [Cl-].C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 CXKWCBBOMKCUKX-UHFFFAOYSA-M 0.000 description 2
- 229960000907 methylthioninium chloride Drugs 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- AXDJCCTWPBKUKL-UHFFFAOYSA-N 4-[(4-aminophenyl)-(4-imino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]aniline;hydron;chloride Chemical compound Cl.C1=CC(=N)C(C)=CC1=C(C=1C=CC(N)=CC=1)C1=CC=C(N)C=C1 AXDJCCTWPBKUKL-UHFFFAOYSA-N 0.000 description 1
- 235000009328 Amaranthus caudatus Nutrition 0.000 description 1
- 240000001592 Amaranthus caudatus Species 0.000 description 1
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- MCTQNEBFZMBRSQ-GEEYTBSJSA-N Chrysoidine Chemical compound Cl.NC1=CC(N)=CC=C1\N=N\C1=CC=CC=C1 MCTQNEBFZMBRSQ-GEEYTBSJSA-N 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- HBQJTBXZMPSVBP-UHFFFAOYSA-N Cyanidine Natural products OC1=CC(=C2/Oc3cc(O)cc(O)c3C=C2O)C=CC1=O HBQJTBXZMPSVBP-UHFFFAOYSA-N 0.000 description 1
- GCPYCNBGGPHOBD-UHFFFAOYSA-N Delphinidin Natural products OC1=Cc2c(O)cc(O)cc2OC1=C3C=C(O)C(=O)C(=C3)O GCPYCNBGGPHOBD-UHFFFAOYSA-N 0.000 description 1
- 239000005867 Iprodione Substances 0.000 description 1
- JBTHDAVBDKKSRW-FMQUCBEESA-N Sudan II Chemical compound CC1=CC(C)=CC=C1\N=N\C1=C(O)C=CC2=CC=CC=C12 JBTHDAVBDKKSRW-FMQUCBEESA-N 0.000 description 1
- FHNINJWBTRXEBC-UHFFFAOYSA-N Sudan III Chemical compound OC1=CC=C2C=CC=CC2=C1N=NC(C=C1)=CC=C1N=NC1=CC=CC=C1 FHNINJWBTRXEBC-UHFFFAOYSA-N 0.000 description 1
- 239000005843 Thiram Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 239000004178 amaranth Substances 0.000 description 1
- 235000012735 amaranth Nutrition 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 150000001449 anionic compounds Chemical class 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- 229930014669 anthocyanidin Natural products 0.000 description 1
- 150000001452 anthocyanidin derivatives Chemical class 0.000 description 1
- 235000008758 anthocyanidins Nutrition 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000012062 aqueous buffer Substances 0.000 description 1
- 239000000987 azo dye Substances 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 239000000981 basic dye Substances 0.000 description 1
- 229960001506 brilliant green Drugs 0.000 description 1
- HXCILVUBKWANLN-UHFFFAOYSA-N brilliant green cation Chemical compound C1=CC(N(CC)CC)=CC=C1C(C=1C=CC=CC=1)=C1C=CC(=[N+](CC)CC)C=C1 HXCILVUBKWANLN-UHFFFAOYSA-N 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 235000014121 butter Nutrition 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- ZXJXZNDDNMQXFV-UHFFFAOYSA-M crystal violet Chemical compound [Cl-].C1=CC(N(C)C)=CC=C1[C+](C=1C=CC(=CC=1)N(C)C)C1=CC=C(N(C)C)C=C1 ZXJXZNDDNMQXFV-UHFFFAOYSA-M 0.000 description 1
- VEVZSMAEJFVWIL-UHFFFAOYSA-O cyanidin cation Chemical compound [O+]=1C2=CC(O)=CC(O)=C2C=C(O)C=1C1=CC=C(O)C(O)=C1 VEVZSMAEJFVWIL-UHFFFAOYSA-O 0.000 description 1
- 235000007242 delphinidin Nutrition 0.000 description 1
- FFNDMZIBVDSQFI-UHFFFAOYSA-N delphinidin chloride Chemical compound [Cl-].[O+]=1C2=CC(O)=CC(O)=C2C=C(O)C=1C1=CC(O)=C(O)C(O)=C1 FFNDMZIBVDSQFI-UHFFFAOYSA-N 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- IINNWAYUJNWZRM-UHFFFAOYSA-L erythrosin B Chemical compound [Na+].[Na+].[O-]C(=O)C1=CC=CC=C1C1=C2C=C(I)C(=O)C(I)=C2OC2=C(I)C([O-])=C(I)C=C21 IINNWAYUJNWZRM-UHFFFAOYSA-L 0.000 description 1
- OXZOLXJZTSUDOM-UHFFFAOYSA-N fluoro 2,2,2-trifluoroacetate Chemical compound FOC(=O)C(F)(F)F OXZOLXJZTSUDOM-UHFFFAOYSA-N 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 239000004009 herbicide Substances 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 229910001412 inorganic anion Inorganic materials 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- ONUFESLQCSAYKA-UHFFFAOYSA-N iprodione Chemical compound O=C1N(C(=O)NC(C)C)CC(=O)N1C1=CC(Cl)=CC(Cl)=C1 ONUFESLQCSAYKA-UHFFFAOYSA-N 0.000 description 1
- 229940098779 methanesulfonic acid Drugs 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- AFAIELJLZYUNPW-UHFFFAOYSA-N pararosaniline free base Chemical compound C1=CC(N)=CC=C1C(C=1C=CC(N)=CC=1)=C1C=CC(=N)C=C1 AFAIELJLZYUNPW-UHFFFAOYSA-N 0.000 description 1
- HKUHOPQRJKPJCJ-UHFFFAOYSA-N pelargonidin Natural products OC1=Cc2c(O)cc(O)cc2OC1c1ccc(O)cc1 HKUHOPQRJKPJCJ-UHFFFAOYSA-N 0.000 description 1
- 235000006251 pelargonidin Nutrition 0.000 description 1
- YPVZJXMTXCOTJN-UHFFFAOYSA-N pelargonidin chloride Chemical compound [Cl-].C1=CC(O)=CC=C1C(C(=C1)O)=[O+]C2=C1C(O)=CC(O)=C2 YPVZJXMTXCOTJN-UHFFFAOYSA-N 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- MCJGNVYPOGVAJF-UHFFFAOYSA-N quinolin-8-ol Chemical compound C1=CN=C2C(O)=CC=CC2=C1 MCJGNVYPOGVAJF-UHFFFAOYSA-N 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- QDRKDTQENPPHOJ-UHFFFAOYSA-N sodium ethoxide Chemical compound [Na+].CC[O-] QDRKDTQENPPHOJ-UHFFFAOYSA-N 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 229940099373 sudan iii Drugs 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000000979 synthetic dye Substances 0.000 description 1
- 239000001040 synthetic pigment Substances 0.000 description 1
- CBXCPBUEXACCNR-UHFFFAOYSA-N tetraethylammonium Chemical class CC[N+](CC)(CC)CC CBXCPBUEXACCNR-UHFFFAOYSA-N 0.000 description 1
- WGHUNMFFLAMBJD-UHFFFAOYSA-M tetraethylazanium;perchlorate Chemical compound [O-]Cl(=O)(=O)=O.CC[N+](CC)(CC)CC WGHUNMFFLAMBJD-UHFFFAOYSA-M 0.000 description 1
- KUAZQDVKQLNFPE-UHFFFAOYSA-N thiram Chemical compound CN(C)C(=S)SSC(=S)N(C)C KUAZQDVKQLNFPE-UHFFFAOYSA-N 0.000 description 1
- 229960002447 thiram Drugs 0.000 description 1
- DTQVDTLACAAQTR-UHFFFAOYSA-N trifluoroacetic acid Substances OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Description
【０００１】[0001]
【産業上の利用分野】本発明は、色素または農薬の分析
方法に関し、詳しくは自由界面電気泳動法により色素ま
たは農薬を分析するにあたり、泳動媒体として有機溶媒
を用いることによって、効率よく分析する方法に関す
る。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for analyzing a dye or a pesticide, and more specifically, a method for efficiently analyzing a dye or a pesticide by using an organic solvent as an electrophoretic medium when the dye or the pesticide is analyzed by a free interface electrophoresis method. Regarding
【０００２】[0002]
【従来の技術および発明が解決しようとする課題】キャ
ピラリー電気泳動法をはじめとする自由界面電気泳動法
は、溶液中における物質の電気的性質を利用した高性
能，高感度の分析方法として、生化学，有機化学，分析
化学等の分野で各種物質の分離、分析技術に用いられて
いる。2. Description of the Related Art Free interface electrophoresis, including capillary electrophoresis, is used as a high-performance, high-sensitivity analytical method that utilizes the electrical properties of substances in a solution. It is used for separation and analysis technology of various substances in fields such as chemistry, organic chemistry, and analytical chemistry.
【０００３】この自由界面電気泳動法では、従来より泳
動媒体（導電媒体）として水を基本とする緩衝液が用い
られている。しかし、この方法では、適用可能な試料
は水溶性のものに限られること、電流に伴うジュール
熱が多量に発生すること、分子量の小さな物質（イオ
ン）は、いわゆるコールラウシュ効果により、分離ピー
クに非対称的な広がりを生じ、感度や分離度が著しく劣
化すること、使用可能なｐＨ領域が限られること、す
なわちｐＨが１以下の強酸性や１３以上の強アルカリ性
の泳動媒体では、電気泳動の電流許容範囲を越えるた
め、このような条件下でのみイオン化する分子種には事
実上適用不能であること等の問題点を抱えていた。In this free interface electrophoresis method, a buffer solution based on water has been used as an electrophoretic medium (conductive medium). However, in this method, applicable samples are limited to water-soluble ones, a large amount of Joule heat is generated with the current, and substances with small molecular weight (ions) are asymmetrical to the separation peak due to the so-called Colelauche effect. Current, the sensitivity and resolution are significantly deteriorated, and the usable pH range is limited. That is, in the case of a strongly acidic electrophoretic medium having a pH of 1 or less and a strongly alkaline electrophoretic medium having a pH of 13 or more, the electrophoretic current is allowed to flow. Since it exceeds the range, there is a problem in that it is practically inapplicable to molecular species that are ionized only under such conditions.
【０００４】そこで、本発明者らは、従来より行われて
いる自由界面電気泳動法における上記課題を解決すべく
検討を重ね、泳動媒体として有機溶媒を使用することが
有効であることを見出し、本発明に到達したのである。Therefore, the present inventors have conducted extensive studies to solve the above-mentioned problems in the conventional free interface electrophoresis method, and found that it is effective to use an organic solvent as a migration medium. The present invention has been reached.
【０００５】[0005]
【課題を解決するための手段】本発明は、自由界面電気
泳動法により色素または農薬を分析するにあたり、泳動
媒体として有機溶媒を用いることを特徴とする色素また
は農薬の分析方法である。The present invention is a method for analyzing a dye or a pesticide characterized by using an organic solvent as an electrophoretic medium when the dye or the pesticide is analyzed by a free interface electrophoresis method.
【０００６】本発明が適用される物質は様々なものがあ
るが、特に色素や農薬（除草剤，殺虫剤，殺菌剤など）
等が好適で、とりわけ、各種食品中に含まれる色素や残
留農薬等の分析、定量に有効である。なお、本発明の方
法は、原理的にこれら物質の他に蛋白質，ペプチド，ア
ミノ酸，有機酸，天然色素，アルカロイド等の生化学物
質や糖類，酸アミド，合成抗菌剤などの分析、定量に利
用することが可能である。There are various substances to which the present invention is applicable, but especially pigments and pesticides (herbicides, insecticides, fungicides, etc.)
Etc. are suitable, and are particularly effective for analysis and quantification of pigments and residual agricultural chemicals contained in various foods. The method of the present invention is used in principle for the analysis and quantification of biochemical substances such as proteins, peptides, amino acids, organic acids, natural pigments, alkaloids, sugars, acid amides, synthetic antibacterial agents, etc. in addition to these substances. It is possible to
【０００７】泳動媒体として用いる有機溶媒は、例えば
メチルアルコール，エチルアルコール，アセトニトリ
ル，アセトン，二硫化炭素などがあり、これらを単独
で、あるいは強酸，強塩基を適宜加えて使用される。The organic solvent used as the electrophoretic medium includes, for example, methyl alcohol, ethyl alcohol, acetonitrile, acetone, carbon disulfide and the like, and these are used alone or by adding a strong acid and a strong base appropriately.
【０００８】有機溶媒を使用することにより、緩衝液イ
オンと試料イオンとの相互作用により生じるピーク感
度，分離度のコールラウシュ的劣化を完全に除くことが
できる。しかも、有機溶媒は通電量が水に比べて著しく
低く抑えられるため、ジュール熱によるピークの形状劣
化や熱拡散による広がりを防いで、分析感度を向上させ
ることができる。By using the organic solvent, it is possible to completely eliminate the Kohllausch-like deterioration of the peak sensitivity and the resolution caused by the interaction between the buffer ions and the sample ions. In addition, since the amount of electricity applied to the organic solvent is significantly lower than that of water, it is possible to prevent deterioration of the peak shape due to Joule heat and spread due to thermal diffusion, and to improve the analytical sensitivity.
【０００９】また、本発明で用いる有機溶媒は、その粘
性係数が水に比べて可なり低いため、水系では事実上実
現困難な低温（０〜１０℃）で高速泳動を行うことが可
能である。なお、水の場合と異なり有機溶媒中ではプロ
トンの授受に伴う電荷の移動が起こらないため、水性の
試料液中でカチオンの分析を行うときは、メタンスルホ
ン酸等の強酸を、アニオンの分析を行うときは、水酸化
ナトリウム，ナトリウムエトキシド等の強塩基をそれぞ
れ用いて試料を予めイオン化することにより、水系緩衝
液中ではイオン化が不可能な物質をイオン化させて分析
することが可能である。Further, since the organic solvent used in the present invention has a viscosity coefficient considerably lower than that of water, it is possible to carry out high-speed migration at a low temperature (0 to 10 ° C.) which is practically difficult to realize in an aqueous system. . Note that, unlike the case of water, charge transfer due to the transfer of protons does not occur in an organic solvent, so when analyzing cations in an aqueous sample solution, analyze strong acids such as methanesulfonic acid and anions. When performing, it is possible to ionize the substance that cannot be ionized in the aqueous buffer solution by previously ionizing the sample using strong bases such as sodium hydroxide and sodium ethoxide, and analyze it.
【００１０】吸光度が小さく通常の分析が困難な金属カ
チオン（Ca2+,Fe2+ ,Ni2+ 等) や無機アニオン(SO
3 2-等）の分析を本発明の方法により行う場合、前者は
例えば４，２−（ピリジルアゾ）−レゾルシノール(PA
R) のような吸光性の強い金属指示薬とのキレート錯イ
オンの形で、また後者は例えばメチレンブルー，パラロ
ーザニリン等のイオン会合試薬とのイオン会合体の状態
で泳動させることにより、水中よりも安定かつ高感度な
分析が可能である。Metal cations (Ca 2+ , Fe 2+ , Ni 2+ etc.) and inorganic anions (SO
3 2- etc.) is analyzed by the method of the present invention, the former is, for example, 4,2- (pyridylazo) -resorcinol (PA
R) in the form of a chelate complex ion with a highly light-absorbing metal indicator, and the latter in the state of an ion association with an ion association reagent such as methylene blue, pararosaniline, etc. Stable and highly sensitive analysis is possible.
【００１１】また、炭化水素等のようにプロトン授受に
与かる官能基をもたない無電荷の非水溶性試料について
は、＋電荷をもつテトラアルキルアンミンイオンとの疎
水的な結合を利用するにより、電気泳動的な分離ができ
る。For an uncharged non-water-soluble sample having no functional group for proton transfer, such as hydrocarbon, the hydrophobic bond with the positively charged tetraalkylammine ion is used. , Electrophoretic separation is possible.
【００１２】[0012]
【実施例】次に、本発明を実施例により詳しく説明す
る。 実施例１ カチオン系色素の分離、分析 下記の条件でキャピラリー電気泳動法を実施した。 キャピラリー：内径７５μｍ，長さ５０ｃｍの溶融シリ
カ管 基本泳動媒体組成：１０Ｎ−トリフルオロ酢酸を0.１％
含むアセトニトリル 供試試料：合成塩基性色素（フクシン，メチルバイオレ
ット，ブリリアントグリーン，メチレンブルー） アントシアニジン系色素（シアニジン，デルフィニジン
ペラルゴニジン） アニリン系脂溶性色素（クリソイジン，バターイエロー
* , オイルイエローOB* )(* は水に不溶性である。） 上記試料を最終濃度で２５ppm になるように５Ｎ−トリ
フルオロ酢酸に溶解して用いた。 泳動条件：１５℃，入口側電極＋，出口側電極グラウン
ド、２５KV，1.０μA 試料の検出：紫外部〜可視部吸光検出器EXAMPLES Next, the present invention will be described in detail with reference to Examples. Example 1 Separation and Analysis of Cationic Dye Capillary electrophoresis was performed under the following conditions. Capillary: fused silica tube with inner diameter of 75 μm and length of 50 cm Basic electrophoretic medium composition: 0.1% of 10N-trifluoroacetic acid
Acetonitrile containing Samples: Synthetic basic dyes (fuchsin, methyl violet, brilliant green, methylene blue) Anthocyanidin dyes (cyanidine, delphinidin pelargonidin) Aniline fat-soluble dyes (chrysoidine, butter yellow)
* , Oil Yellow OB * ) ( * is insoluble in water.) The above sample was dissolved in 5N-trifluoroacetic acid to a final concentration of 25 ppm and used. Electrophoresis conditions: 15 ° C, inlet side electrode +, outlet side electrode ground, 25KV, 1.0μA Sample detection: UV-visible absorption detector
【００１３】その結果、図１に示したように、供試した
すべてのカチオン系色素は２０分以内に極めて効率よく
分離でき、ピークより算出した理論段数は、数万〜数十
万、検出感度は数十ｐｐｂであった。As a result, as shown in FIG. 1, all the cationic dyes tested could be separated very efficiently within 20 minutes, the theoretical plate number calculated from the peak was tens of thousands to hundreds of thousands, and the detection sensitivity was Was several tens of ppb.
【００１４】実施例２ アニオン系色素の分離、分析 下記の条件でキャピラリー電気泳動法を実施した。 キャピラリー：内径７５μｍ，長さ５０ｃｍの溶融シリ
カ管 基本泳動媒体組成：６Ｎ−ＮａＯＨを0.１％含むアセト
ニトリルあるいはこれにテトラエチルアンモニウム塩を
数十ミリモル溶解させたもの 供試試料：酸性アゾ色素（アマランス，エリスロシン，
アルラレッド−ＡＣ）水不溶性の脂溶性アゾナフトール
色素（スダン−Ｉ，スダン−II,スダン−III) 上記試料を最終濃度で２５ppm になるように６Ｎ−Ｎａ
ＯＨ／水に溶解して用いた。 泳動条件：１５℃，入口側電極−，出口側電極グラウン
ド、２５KV，1.０μA 試料の検出：紫外部〜可視部吸光検出器Example 2 Separation and Analysis of Anionic Dye Capillary electrophoresis was carried out under the following conditions. Capillary: fused silica tube having an inner diameter of 75 μm and a length of 50 cm Basic electrophoretic medium composition: acetonitrile containing 0.1% of 6N-NaOH or tens of millimoles of tetraethylammonium salt dissolved Sample sample: acidic azo dye (Amaranth , Erythrosin,
Arla red-AC) Water-insoluble fat-soluble azonaphthol dyes (Sudan-I, Sudan-II, Sudan-III) 6N-Na was added to the above sample so that the final concentration was 25 ppm.
Used by dissolving in OH / water. Electrophoresis conditions: 15 ° C, inlet electrode-, outlet electrode ground, 25KV, 1.0μA Sample detection: UV-visible absorption detector
【００１５】その結果、図２に示したように、供試した
すべてのアニオン系色素は３０分以内に極めて効率よく
分離でき、実施例１と同様に理論段数は、数万〜数十
万、検出感度は数十ｐｐｂであった。As a result, as shown in FIG. 2, all the anionic dyes tested could be separated very efficiently within 30 minutes, and the theoretical plate number was several tens to several hundreds of thousands as in Example 1. The detection sensitivity was several tens of ppb.
【００１６】実施例３ 無電荷，非水溶性成分の分離、分析 下記の条件でキャピラリー電気泳動法を実施した。 キャピラリー：内径７５μｍ，長さ５０ｃｍの溶融シリ
カ管 基本泳動媒体組成：２０ｍＭテトラフルオロ酢酸，２５
ｍＭテトラエチルアンモニウムパークロレートを含むア
セトニトリル 供試試料：殺虫剤・殺菌剤（アシュラム* , イプロジオ
ン, ベンスリド，チラム，テトラエチルチラム, オキシ
ン銅） 脂溶性合成色素（バターイエロー* ，イエローＯＢ* ，
スダン−Ｉ）（* は水に不溶性である。） 泳動条件：２３℃，入口側電極＋，出口側電極グラウン
ド、２５KV 試料の検出：紫外部〜可視部吸光検出器Example 3 Separation and analysis of uncharged and water-insoluble components Capillary electrophoresis was carried out under the following conditions. Capillary: fused silica tube with inner diameter of 75 μm and length of 50 cm Basic electrophoretic medium composition: 20 mM tetrafluoroacetic acid, 25
Acetonitrile containing mM tetraethylammonium perchlorate Samples: Insecticides / bactericides (Ashram * , Iprodione, Bencelide, Thiram, Tetraethyltyram, Oxin copper) Fat-soluble synthetic pigments (Butter Yellow * , Yellow OB * ,
Sudan-I) ( * is insoluble in water.) Electrophoresis conditions: 23 ° C, inlet side electrode +, outlet side electrode ground, 25KV Sample detection: UV-visible absorption detector
【００１７】その結果、図３に示したように、電荷をも
たない９成分がすべて効率よく分離できた。また、理論
段数は、数万、検出感度は数十ｐｐｍであった。As a result, as shown in FIG. 3, all 9 components having no electric charge could be efficiently separated. The theoretical plate number was tens of thousands, and the detection sensitivity was tens of ppm.
【００１８】[0018]
【発明の効果】本発明の方法によれば、ピークの泳動速
度およびピーク面積の再現性は１〜２％の範囲に収ま
り、自由界面電気泳動法を食品等に含まれる色素や農薬
等の定量分析法として適用することが可能である。特
に、従来の水系泳動媒体を用いる電気泳動法では分離、
分析が不可能であった脂溶性合成色素について、高い理
論段数および再現性をもって分離、分析できることは画
期的なことである。According to the method of the present invention, the migration rate of peaks and the reproducibility of peak areas are within the range of 1 to 2%, and free interface electrophoresis is used to quantify pigments and agricultural chemicals contained in foods. It can be applied as an analytical method. Especially, in the electrophoresis method using the conventional aqueous migration medium, separation,
It is epoch-making that lipophilic synthetic dyes that could not be analyzed can be separated and analyzed with a high theoretical plate number and reproducibility.
【図１】 カチオン系色素の分離、分析結果を示す。FIG. 1 shows the results of separation and analysis of cationic dyes.
【図２】 アニオン系色素の分離、分析結果を示す。FIG. 2 shows the results of separation and analysis of anionic dyes.
【図３】 無電荷、非水溶性成分の分離、分析結果を示
す。FIG. 3 shows the results of separation and analysis of uncharged and water-insoluble components.
Claims (4)
薬を分析するにあたり、泳動媒体として有機溶媒を用い
ることを特徴とする色素または農薬の分析方法。1. A method for analyzing a dye or a pesticide, which comprises using an organic solvent as an electrophoretic medium when the dye or the pesticide is analyzed by a free interface electrophoresis method.
である請求項１記載の方法。2. The method according to claim 1, wherein the pigment or the agricultural chemical is contained in the food.
ルコール，アセトニトリル，アセトンおよび二硫化炭素
の中から選ばれた物質を含むものである請求項１記載の
方法。3. The method according to claim 1, wherein the organic solvent contains a substance selected from methyl alcohol, ethyl alcohol, acetonitrile, acetone and carbon disulfide.
泳動法である請求項１記載の方法。4. The method according to claim 1, wherein the free interface electrophoresis method is a capillary electrophoresis method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5222766A JPH0820416B2 (en) | 1993-08-17 | 1993-08-17 | Dye or pesticide analysis method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5222766A JPH0820416B2 (en) | 1993-08-17 | 1993-08-17 | Dye or pesticide analysis method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0755769A JPH0755769A (en) | 1995-03-03 |
| JPH0820416B2 true JPH0820416B2 (en) | 1996-03-04 |
Family
ID=16787566
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5222766A Expired - Lifetime JPH0820416B2 (en) | 1993-08-17 | 1993-08-17 | Dye or pesticide analysis method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0820416B2 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE0201655D0 (en) * | 2002-05-31 | 2002-05-31 | Amersham Biosciences Ab | A method of electrophoresis |
| US6939453B2 (en) * | 2002-08-14 | 2005-09-06 | Large Scale Proteomics Corporation | Electrophoresis process using ionic liquids |
-
1993
- 1993-08-17 JP JP5222766A patent/JPH0820416B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0755769A (en) | 1995-03-03 |
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