JPH082294B2 - Culture medium of Chlamydomonas single-cell green algae Earl sager strain 95 - Google Patents
Culture medium of Chlamydomonas single-cell green algae Earl sager strain 95Info
- Publication number
- JPH082294B2 JPH082294B2 JP63235958A JP23595888A JPH082294B2 JP H082294 B2 JPH082294 B2 JP H082294B2 JP 63235958 A JP63235958 A JP 63235958A JP 23595888 A JP23595888 A JP 23595888A JP H082294 B2 JPH082294 B2 JP H082294B2
- Authority
- JP
- Japan
- Prior art keywords
- strain
- culture medium
- earl
- chlamydomonas
- sager
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
Landscapes
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
【発明の詳細な説明】 ［産業上の利用分野］ 本発明はクラミドモナス属単細胞緑藻アール サガー
ストレーン95の培養基に関する。DETAILED DESCRIPTION OF THE INVENTION [Industrial application] The present invention relates to a culture medium of Chlamydomonas unicellular green alga Earl Sager strain 95.
［従来の技術］ 単細胞緑藻の一種としてはクロレラがあるが、この培
養地として、炭素源としては、炭酸ガス；酢酸、クエン
酸、コハク酸、その他の有機酸やそれらの塩；グルコー
ス、シュークロース、‐・・‐、窒素源としてはアンモ
ニウム塩、尿酸、窒素を含むアミノ酸、無機源としては
リン、イオウ、カリウム、マグネシウム、鉄、マンガ
ン、コバルト、モリブデン、亜鉛、銅等を用いている。
炭素源としては0.1〜10重量％、窒素源、無機源ともに
１重量％以下である（特開昭59−192083）。[Prior Art] Chlorella is one of the unicellular green algae. As the culture medium, carbon dioxide is used as a carbon source; acetic acid, citric acid, succinic acid, other organic acids and salts thereof; glucose, sucrose. , ---, an ammonium salt, uric acid, an amino acid containing nitrogen is used as a nitrogen source, and phosphorus, sulfur, potassium, magnesium, iron, manganese, cobalt, molybdenum, zinc, copper, etc. are used as an inorganic source.
The carbon source is 0.1 to 10% by weight, and both the nitrogen source and the inorganic source are 1% by weight or less (JP-A-59-192083).
本発明にかかるクラミドモナス属単細胞緑藻アール
サガー ストレーン95の培養基につき、各種微量の化学
成分を選び増殖（生育速度）の最もすぐれたものを研究
を重ねた結果得られた。Chlamydomonas single-cell green algae according to the present invention
It was obtained as a result of repeated studies on the culture medium of sager strain 95, selecting various trace amounts of chemical components and having the best growth (growth rate).
［発明が解決しようとする課題］ 本発明にかかるクラミドモナス属単細胞緑藻は詳しく
はクラミドモナスラインハルデイ（Chlamydomonas Rein
hardii）、緑藻綱（Chlorophyceae）、オオヒゲマワリ
目（Volvocalse）、株名アール サガー ストレーン95
（R.Sager strain 95）で、光合成色素、むち形鞭毛を
有する単細胞緑藻の一種であり、ATCC No.18302で、以
下クラミドモナスと略称する。[Problems to be Solved by the Invention] The unicellular green alga of the genus Chlamydomonas according to the present invention is specifically described in Chlamydomonas Rein
hardii), Chlorophyceae, Chlorophyceae, Volvocalse, strain name Earl Sagar Strain 95
(R. Sager strain 95), a kind of unicellular green alga having a photosynthetic pigment and whiplash flagella, which is hereinafter abbreviated as Chlamydomonas in ATCC No. 18302.
本発明はクラミドモナスの培養に最も適した培養基を
見当することである。The present invention is to find the most suitable culture medium for culturing Chlamydomonas.
［課題を解決するための手段］ クラミドモナス属単細胞緑藻アール サガーストレー
ン95を培養するにあたり、ストックＡとしてK2HPO4の水
溶液、ストックＢとしてNH4Cl、MgSO4・7H2O、CaCl2・2
H2Oの水溶液、ストックＣとしてEDTA（C10H16N2O8）、Z
nSO4・7H2O、H3BO3、MnCl2・4H2O、FeSO4・7H2O、CoCl2
・6H2O、CuSO4・5H2O、(NH4)6(Mo7)O24・4H2Oの水溶液
を予め用意し、培養するにあたり、それらを混合してな
ることを特徴とするクラミドモナス属単細胞緑藻アール
サガー ストレーン95の培養基。[Means for Solving the Problems] When culturing Chlamydomonas single-cell green algae Earl sagar strain 95, an aqueous solution of K 2 HPO 4 as stock A and NH 4 Cl, MgSO 4 / 7H 2 O, CaCl 2 .2 as stock B are used.
Aqueous solution of H 2 O, EDTA (C 10 H 16 N 2 O 8 ) as stock C, Z
nSO 4 / 7H 2 O, H 3 BO 3 , MnCl 2 / 4H 2 O, FeSO 4 / 7H 2 O, CoCl 2
· 6H 2 O, CuSO 4 · 5H 2 O, Chlamydomonas of (NH 4) 6 (Mo 7 ) O 24 · 4H 2 O-solution of the previously prepared, upon culturing, characterized by comprising a mixture of them Culture medium for genus single-cell green algae Earl Sagar strain 95.
［作用］ クラミドモナス属単細胞緑藻アール サガー ストレ
ーン95の培養基として以下に示す実施例１のものが生育
速度が最もい。[Operation] As a culture medium for the Chlamydomonas unicellular green algae Earl Sagar strain 95, the culture medium of Example 1 shown below has the highest growth rate.
［実施例］ 実施例１ クラミドモナスの培養には培養基として ストックＡ K2HPO4 108.0g/l ストックＢ NH4Cl 5.0g/l MgSO4・7H2O 2.0g/l CaCl2・2H2O 1.0g/l ストックＣ EDTA(C10H16N2O8) 5.0g/l ZnSO4・7H2O 2.2g/l H3BO3 1.14g/l MnCl2・4H2O 0.51g/l FeSO4・7H2O 0.5 g/l CoCl2・6H2O 0.16g/l CuSO4・5H2O 0.16g/l (NH4)6(Mo7)O24・2H2O 0.11g/l 水 970ml 以上３種の溶液を予め作成し、使用の場合に ストックＡ 10ml/l ストックＢ 10ml/l ストックＣ 10ml/l の割合で培養基を作成し、クラミドモナス3.5×103cell
s/mlを入れると４日で５×106cells/mlとなる。EXAMPLES Stock A K 2 HPO 4 as a culture medium for culture of Example 1 Chlamydomonas 108.0 g / l Stock B NH 4 Cl 5.0g / l MgSO 4 · 7H 2 O 2.0g / l CaCl 2 · 2H 2 O 1.0 g / l Stock C EDTA (C 10 H 16 N 2 O 8 ) 5.0 g / l ZnSO 4 / 7H 2 O 2.2 g / l H 3 BO 3 1.14 g / l MnCl 2 4H 2 O 0.51 g / l FeSO 4・ 7H 2 O 0.5 g / l CoCl 2・ 6H 2 O 0.16g / l CuSO 4・ 5H 2 O 0.16g / l (NH 4 ) 6 (Mo 7 ) O 24・ 2H 2 O 0.11g / l Water 970ml or more Three types of solutions were prepared in advance, and when used, a culture medium was prepared at a ratio of stock A 10 ml / l stock B 10 ml / l stock C 10 ml / l, and Chlamydomonas 3.5 × 10 3 cell was prepared.
When s / ml is added, it becomes 5 × 10 6 cells / ml in 4 days.
Claims (1)
ーストレーン95を培養するにあたり、ストックＡとして
K2HPO4の水溶液、ストックＢとしてNH4Cl、MgSO4・7H
2O、CaCl2・2H2Oの水溶液、ストックＣとしてEDTA（C10
H16N2O8）、ZnSO4・7H2O、H3BO3、MnCl2・4H2O、FeSO4
・7H2O、CoCl2・6H2O、CuSO4・5H2O、(NH4)6(Mo7)O24・
4H2Oの水溶液を予め用意し、培養するにあたり、それら
を混合してなることを特徴とするクラミドモナス属単細
胞緑藻アール サガー ストレーン95の培養基。1. As a stock A for culturing Chlamydomonas single-cell green alga Earl Sager strain 95
Aqueous solution of K 2 HPO 4 , NH 4 Cl as stock B, MgSO 4 · 7H
2 O, CaCl 2 · 2H 2 O aqueous solution, EDTA (C 10
H 16 N 2 O 8 ), ZnSO 4 / 7H 2 O, H 3 BO 3 , MnCl 2 4H 2 O, FeSO 4
・ 7H 2 O, CoCl 2・ 6H 2 O, CuSO 4・ 5H 2 O, (NH 4 ) 6 (Mo 7 ) O 24・
A culture medium for Chlamydomonas single-cell green alga Earl Sager strain 95, which is prepared by preparing an aqueous solution of 4H 2 O in advance and culturing them.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63235958A JPH082294B2 (en) | 1988-09-20 | 1988-09-20 | Culture medium of Chlamydomonas single-cell green algae Earl sager strain 95 |
| KR1019890000069A KR900004931A (en) | 1988-09-20 | 1989-01-06 | Incubator and Culture Method of Chlamydomonas Single Cell Green Alga Strain Strain 95 |
| JP19338989A JPH03130070A (en) | 1988-09-20 | 1989-07-26 | Culture of unicellular green alga r. sager strain 95 belonging to genus chlamydomonas and culture device therefor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63235958A JPH082294B2 (en) | 1988-09-20 | 1988-09-20 | Culture medium of Chlamydomonas single-cell green algae Earl sager strain 95 |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP19338989A Division JPH03130070A (en) | 1988-09-20 | 1989-07-26 | Culture of unicellular green alga r. sager strain 95 belonging to genus chlamydomonas and culture device therefor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH02154682A JPH02154682A (en) | 1990-06-14 |
| JPH082294B2 true JPH082294B2 (en) | 1996-01-17 |
Family
ID=16993734
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP63235958A Expired - Lifetime JPH082294B2 (en) | 1988-09-20 | 1988-09-20 | Culture medium of Chlamydomonas single-cell green algae Earl sager strain 95 |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JPH082294B2 (en) |
| KR (1) | KR900004931A (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101339686B1 (en) * | 2011-06-08 | 2013-12-10 | 대상 주식회사 | Process for preparing chlorella with high content of lutein |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5231885A (en) * | 1975-09-04 | 1977-03-10 | Mitsubishi Chem Ind Ltd | Method for accelerating propagation of fine alga |
| JPS59192083A (en) * | 1983-04-15 | 1984-10-31 | Asahi Glass Co Ltd | Preparation of unicellular green alga |
-
1988
- 1988-09-20 JP JP63235958A patent/JPH082294B2/en not_active Expired - Lifetime
-
1989
- 1989-01-06 KR KR1019890000069A patent/KR900004931A/en not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| KR900004931A (en) | 1990-04-13 |
| JPH02154682A (en) | 1990-06-14 |
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