JPH089523B2 - Plant rooting promoter - Google Patents

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a rooting promoter containing an adenine derivative or a salt thereof as an active ingredient.

[0002]

2. Description of the Related Art Conventionally, methods such as cutting and harvesting have been used for the propagation of plants, and in recent years, a growth method by plant tissue culture has also come to be used. There is. In order to grow plants using these methods, rooting is an essential condition. In many cases, it is known that rooting is further improved by using auxins. However, it is well known that there are many plants in which it is difficult or impossible to promote rooting even by this method. Especially in woody plants, it is often difficult to root. An object of the present invention is to obtain an excellent rooting promoter that overcomes such drawbacks.

[0003]

[Means for Solving the Problems]
It was found that the adenine derivative represented by the general formula (1) and a salt thereof have a strong cytokinin activity (JP-A-5-112569). The present inventors, as a result of further studying the action and effect on plants for these compounds, not only have cytokinin activity,
Unexpectedly found to have excellent rooting promoting action,
The present invention has been completed.

That is, the present invention is a rooting promoter containing at least one member selected from the group consisting of adenine derivatives represented by the general formula of Chemical formula 2 and salts thereof. Hereinafter, the present invention will be specifically described.

[0005]

[Chemical 2] (N is 1 or 2, R and R'represent hydrogen or a methyl group)

As a typical example of the adenine derivative represented by the general formula of Chemical Formula 2 according to the present invention, N ^6- [2
-(N-methoxyimino) ethyl] adenine, N of Chemical formula 4
⁶ - [2- (N- methoxyimino) propyl] adenine, N ⁶ of reduction 5 - [3- (N- hydroxyimino) butyl] adenine, N ⁶ of reduction 6 - [3- (N- methoxyimino) butyl ] Adenine is mentioned.

[0007]

[Chemical 3]

[0008]

[Chemical 4]

[0009]

[Chemical 5]

[0010]

[Chemical 6]

As a method for treating this agent, the cuttings are soaked in an aqueous solution of this agent, and then inserted into a bed, a drug is applied to the cut portion of the cuttings or dressed, or this agent is used as a carrier. It is mixed with and formulated into an aqueous solution, emulsion, wettable powder, water solution, powder, granules, tablets, ointment, etc., or as it is or diluted with a general extender, that is, a liquid or solid diluent, and foliage Methods such as spraying, coating or dressing, and dipping are effective. In addition, this agent can further promote root growth even by a short-time immersion treatment before rooting.

As the carrier, fine powder of an inert mineral substance, an inert organic solvent, water or the like which is usually used for a rooting promoter for plants is used. Further, various surfactants, polymer compounds and the like are appropriately used for the purpose of improving the properties of the preparation or enhancing the effect of the drug.

Other rooting agents such as plant hormones indoleacetic acid, indolebutyric acid, naphthylacetic acid and 2,4-dichlorophenoxyacetic acid can also be mixed with fungicides and fertilizers. The effect of can be improved and labor can be saved.

In tissue culture, rooting can be carried out by a method of adding the agent of the present invention to the medium, or by immersing the plant tissue in an aqueous solution of the agent of the present invention and then transferring it to the medium. The medium may be any medium as long as it contains a carbon source, a nitrogen source, an inorganic substance, and the like, and specific examples of the medium include Murashige-Skoog's medium (MS medium) and Rinsmeier-Skoog's medium (LS medium). , Gamborg
B5 medium (B5 medium), Woody plant medium (WP
Medium) and Chalupa's BT medium (BT medium) and the like, or those obtained by making various modifications using these as basic media. Examples will be described below.

[0015]

EXAMPLES Example 1 A Knugyshte that had been repeatedly subcultured aseptically was inserted into a WP medium (0.3% cellulite, 1% sucrose) containing a test compound at a predetermined concentration, and the temperature was adjusted to 25 ± 1 ° C. 4,000
The cells were cultured in a 16-hour light period and a 8-hour dark period for 8 weeks.
The results are shown in Table 1.

[0016]

[Table 1] Table 1 N ^6- [2- (N-methoxyimino) propyl] adenine (MPA) accelerating effect on rooting from Kunugi shoot Test compound concentration (mg / l) Number of planted roots Number of roots Ratio (%) MPA 0.02 81 51 63 63 0.05 92 53 53 58 0.1 94 48 51 BA 0.1 88 1 1 IBA 0.1 75 25 33 33 BA: benzyladenine IBA: indolebutyric acid Rooting rate = ( Rooting number / planting number) × 100

Example 2 Soybean seeds (cultivar: Enrei) were sown in a vegetable soil and grown in the field for 11 days. We chose soybean with completely developed primary leaves. 5 to 10 mm above the cotyledon was cut off, and above the primary leaf was cut out. 1/20 Hoagland hydroponics (30m
Into a 50 ml vial containing 1), 3 epicotyls with 2 primary leaves were inserted. Cover the vial with aluminum foil to protect it from light,
The sample was left under the condition of 7,000 Lux for 7 days, treated with MPA on the way, and its effect was investigated.

The treatment with MPA is carried out by immersing the lower hypocotyl in a 1/20 Hoagland hydroponic solution (10 ml) containing a predetermined concentration of MPA for 3 hours.
The culture conditions were the same before and after the treatment except that PA was added. Hoagland's hydroponics was replaced daily. Seven days after the preparation of the test material (supercotyl), the fresh weight of roots was examined. Table 2 shows the results. Rooting of all test plots was observed 5 days after the preparation of the test material (supercotyl).

[0019]

[Table 2] Table 2 MPA promoting effect on rooting from epicotyl of soybean Timing of treatment ^{* 1} MPA concentration Root fresh weight ^{* 2} (hr) (mg / l) (mg / explant) 0 146 (100) 0−3 0.002 169 (116) 0.02 187 (128) 22−25 0.002 136 (93) 0.02 133 (91) 45−48 0.002 163 (112) 0.02 141 (97) 69−72 0.002 177 (121) 0.02 153 ( 105) 93-96 0.002 178 (122) 0.02 180 (113) Number of data n = 6 (untreated: n = 9) * 1 Time when treatment was performed with the time immediately after preparation of the test material (upper hypocotyl) set to 0 showed that. * 2 The value in parentheses is a value when the untreated weight is 100.

Example 3 Soybean seeds (variety: Enrei) were sown in a vegetable soil and grown in the field for 14 days. We chose soybean with completely developed primary leaves. 5 to 10 mm above the cotyledon was cut off, and above the primary leaf was cut out. Predetermined concentration of 2,4-D or M
Three epicotyls with two primary leaves were placed in a 30 ml vial containing an aqueous solution containing PA (20 ml). The tube bottle was covered with aluminum foil to protect it from light. After 8 hours under conditions of 25 ° C and 7,000 Lux, the solution was 1/20 Hoagland's hydroponic solution (3
0 ml) and placed under the same conditions. Hoagland's hydroponics was replaced daily. After left for 7 days, the fresh weight of roots was examined.

[0021]

[Table 3] Table 3 Promoting effects of 2,4-D ^{* 1} and MPA on rooting from soybean epicotyl 2,4-D concentration MPA concentration Fresh weight of root ^{* 2} (mg / l) (mg / l ) (mg / explant) 0 0 139 (100) 0 0.004 164 (118) 0.02 0 200 (144) 0.02 0.004 223 (160) Number of data n = 6 * 1 2,4-D: 2,4-Dichlorophenoxyacetic Acid * 2 Figures in parentheses are values when the fresh weight is 100 when untreated.

Example 4 Soybean seeds (variety: Enrei) were dipped in antiformin containing 1% available chlorine for 12 minutes, and then washed with sterilized water 6 times. After adding 15 ml of 1.6% agar to a test tube having a diameter of 25 mm and making a groove on the surface with tweezers, one seed was placed and placed in the dark at 30 ° C. for 5 days. From the germinated and grown soybean hypocotyl, a 1 mm thick section was cut. To a glass vial having an inner diameter of 26 mm, 10 ml of Miller's medium (containing 1.0% by weight of agar) containing 0.02 mg / l kinetin and 0.4 mg / l 2,4-D was added. A set of 4 sections was placed on the medium and placed in the dark at 30 ° C. for 2 weeks. The callus derived from the section was placed on a mirror medium containing a test compound at a predetermined concentration and 0.1 mg / l 2,4-D, and the number of redifferentiated roots was counted.

[0023]

[Table 4] Table 4 MPA accelerating effect on root redifferentiation from soybean callus Test compound concentration Number of roots per incubator (mg / l) 0 0 MPA 0.02 2.3 TG-19 0.020 TG -19: Adenine derivative showing cytokinin activity similar to MPA [Journal of the Crop Science Society of Japan, Vol. 60 (Annex 2) p. 149]

Example 5 Callus was induced by placing the shoot apices of asparagus (variety: Mary Washington 500 W) young stems on MS medium containing 1 mg / l 2,4-D. The above callus after repeated passages contained M containing 1 mg / l of the test compound and no auxin.
It was shake-cultured in S liquid medium at 25 ° C. under continuous light.
As a result, among the four compounds tested (MPA, BA, kinetin, KT-30), clear redifferentiation of adventitious roots was observed only in the callus of the MPA-added medium.

[0025]

[Table 5] Table 5 Effect of various cytokinins on redifferentiation from asparagus callus Compound concentration Number of roots per incubator (mg / l) KT-30 10 BA 1 0 kinetin 1 0.1 MPA 1 2.6 KT -30: 1- (2-chloro-4-pyridyl) -3-
Phenylurea

[0026]

EFFECTS OF THE INVENTION The adenine derivative of the present invention or a salt thereof can easily promote rooting of plants.

[0027]

[Brief description of drawings]

1 is a photograph as a substitute for a drawing showing the morphology of an organism, showing the rooting situation from Kunugi shoots in Example 1. FIG.

[Explanation of symbols]

MPA: N ^6- [2- (N-methoxyimino) propyl] adenine BA: benzyladenine

Claims (1)

[Claims] 1. A rooting promoter containing the adenine derivative represented by Chemical formula 1 or a salt thereof as an active ingredient. [Chemical 1] (N is 1 or 2, R and R'represent hydrogen or a methyl group)