Sanchez-Gonzalez et al., 2025 - Google Patents
Injectable cell-laden gelatin-chondroitin sulphate hydrogels for liver in vitro modelsSanchez-Gonzalez et al., 2025
View HTML- Document ID
- 6732213371540850423
- Author
- Sanchez-Gonzalez E
- Naranjo-Alcazar R
- Tort-Ausina I
- Donato M
- Salmeron-Sanchez M
- Tolosa L
- Gallego-Ferrer G
- Publication year
- Publication venue
- International Journal of Biological Macromolecules
External Links
Snippet
Liver extracellular matrix-based models that precisely reproduce liver physiology and functions are required as 3D culture microenvironments for multiple applications in toxicology and metabolism, or for understanding the mechanisms implicated in liver disease …
- 239000000017 hydrogel 0 title abstract description 94
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
- G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
- G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues ; Not used, see subgroups
- C12N5/0602—Vertebrate cells
- C12N5/067—Hepatocytes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
- G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
- G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/70—Polysaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/54—Collagen; Gelatin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Rizwan et al. | Designing hydrogels for 3D cell culture using dynamic covalent crosslinking | |
| Bokhari et al. | Culture of HepG2 liver cells on three dimensional polystyrene scaffolds enhances cell structure and function during toxicological challenge | |
| Nicolas et al. | 3D extracellular matrix mimics: fundamental concepts and role of materials chemistry to influence stem cell fate | |
| Cuvellier et al. | 3D culture of HepaRG cells in GelMa and its application to bioprinting of a multicellular hepatic model | |
| Elomaa et al. | Development of GelMA/PCL and dECM/PCL resins for 3D printing of acellular in vitro tissue scaffolds by stereolithography | |
| Caliari et al. | A practical guide to hydrogels for cell culture | |
| Koivisto et al. | Mechanically biomimetic gelatin–gellan gum hydrogels for 3D culture of beating human cardiomyocytes | |
| Prestwich | Evaluating drug efficacy and toxicology in three dimensions: using synthetic extracellular matrices in drug discovery | |
| Dutta et al. | Cell-interactive 3D-scaffold; advances and applications | |
| US8932858B2 (en) | Modified polysaccharide for cell culture and release | |
| Sk et al. | Synthesis and characterization of site selective photo-crosslinkable glycidyl methacrylate functionalized gelatin-based 3D hydrogel scaffold for liver tissue engineering | |
| US12123026B2 (en) | Human pluripotent stem cell-derived brain organoids for cancer modeling and drug screening | |
| US20190106673A1 (en) | Bioink compositions and methods of preparing and using the same | |
| Tam et al. | Transparent porous polysaccharide cryogels provide biochemically defined, biomimetic matrices for tunable 3D cell culture | |
| Sakai et al. | Comparative analysis of gene expression in rat liver tissue and monolayer-and spheroid-cultured hepatocytes | |
| Dudaryeva et al. | Tunable bicontinuous macroporous cell culture scaffolds via kinetically controlled phase separation | |
| Shen et al. | Fabrication of collagen gel hollow fibers by covalent cross-linking for construction of bioengineering renal tubules | |
| Wang et al. | High-throughput hyaluronic acid hydrogel arrays for cell selective adhesion screening | |
| Sasikumar et al. | Influence of liver extracellular matrix in predicting drug-induced liver injury: an alternate paradigm | |
| Sanchez-Gonzalez et al. | Injectable cell-laden gelatin-chondroitin sulphate hydrogels for liver in vitro models | |
| Wang et al. | Extracellular matrix functionalization and Huh-7.5 cell coculture promote the hepatic differentiation of human adipose-derived mesenchymal stem cells in a 3D ICC hydrogel scaffold | |
| Darakhshan et al. | Generation of scalable hepatic micro-tissues as a platform for toxicological studies | |
| Itle et al. | Hepatocyte viability and protein expression within hydrogel microstructures | |
| Asthana et al. | Evaluation of cellular adhesion and organization in different microporous polymeric scaffolds | |
| Fernández‐Pérez et al. | Highly tunable and cell‐remodelable thiol‐ene alginate‐peptide crosslinked hydrogels to recreate cellular and organoid microenvironments for biofabrication |