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NZ761782B2 - Apparatus for conducting an assay - Google Patents
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NZ761782B2 - Apparatus for conducting an assay - Google Patents

Apparatus for conducting an assay Download PDF

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Publication number
NZ761782B2
NZ761782B2 NZ761782A NZ76178218A NZ761782B2 NZ 761782 B2 NZ761782 B2 NZ 761782B2 NZ 761782 A NZ761782 A NZ 761782A NZ 76178218 A NZ76178218 A NZ 76178218A NZ 761782 B2 NZ761782 B2 NZ 761782B2
Authority
NZ
New Zealand
Prior art keywords
microfluidic system
magnet
assay
turntable
optionally
Prior art date
Application number
NZ761782A
Other versions
NZ761782A (en
Inventor
David Hugh Williams
Kenneth Wong
Original Assignee
Mast Group Limited
Filing date
Publication date
Priority claimed from GBGB1711804.3A external-priority patent/GB201711804D0/en
Application filed by Mast Group Limited filed Critical Mast Group Limited
Publication of NZ761782A publication Critical patent/NZ761782A/en
Publication of NZ761782B2 publication Critical patent/NZ761782B2/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0803—Disc shape
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1838—Means for temperature control using fluid heat transfer medium
    • B01L2300/1844—Means for temperature control using fluid heat transfer medium using fans
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/50—Clamping means, e.g. tongs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
    • B01L9/527—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips for microfluidic devices, e.g. used for lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/025—High gradient magnetic separators
    • B03C1/031—Component parts; Auxiliary operations
    • B03C1/033—Component parts; Auxiliary operations characterised by the magnetic circuit
    • B03C1/0332—Component parts; Auxiliary operations characterised by the magnetic circuit using permanent magnets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/28—Magnetic plugs and dipsticks
    • B03C1/288—Magnetic plugs and dipsticks disposed at the outer circumference of a recipient
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/18—Magnetic separation whereby the particles are suspended in a liquid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/26—Details of magnetic or electrostatic separation for use in medical or biological applications
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N2035/00099—Characterised by type of test elements
    • G01N2035/00158—Elements containing microarrays, i.e. "biochip"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/0439—Rotary sample carriers, i.e. carousels
    • G01N2035/0444—Rotary sample carriers, i.e. carousels for cuvettes or reaction vessels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/72—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating magnetic variables
    • G01N27/74—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating magnetic variables of fluids
    • G01N27/745—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating magnetic variables of fluids for detecting magnetic beads used in biochemical assays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N35/00069—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides whereby the sample substrate is of the bio-disk type, i.e. having the format of an optical disk
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/025—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes

Abstract

The present invention provides an apparatus for conducting an assay in a microfluidic system comprising magnetic beads, said apparatus comprising: a platform upon which a microfluidic system can be mounted, one or more actuators having a magnet mounted directly or mounted on a spring, a spring assembly or an adjustable mounting assembly; wherein the one or more actuators comprise at least one rotary actuator, wherein the actuator is configured to enable acruate movement of the magnet in an X and Y axis; wherein said one or more actuators is configured to directly influence movement of magnetic beads housed within a microfluidic system when a microfluidic system is mounted on said platform, and a control means configured to control relative movement of the one or more magnets, and a microfluidic system when mounted, to enable the magnet to trace a desired path across a mounted microfluidic system, said magnet being positionable at any x- and y-coordinates of a mounted microfluidic system, wherein said apparatus further comprises a means for moving a mounted microfluidic system in a stepwise fashion. Conventional assay discs utilised magnetic beads that were controlled by centrifugal force or externally mounted magnets. The current invention aims to improve the control of such magnetic beads.

Claims (21)

1. An apparatus for conducting an assay in a microfluidic system comprising magnetic beads, said apparatus comprising: a) a platform upon which the microfluidic system can be mounted, b) one or more actuators; c) wherein the one or more actuators comprise a magnet mounted directly thereupon, or mounted on a spring, a spring assembly or an adjustable mounting assembly; wherein the one or more actuators comprises at least one rotary actuator, wherein the at least one rotary actuator is configured to enable arcuate movement of the magnet in an X and Y axis, wherein said one or more actuators is configured to directly influence movement of magnetic beads housed within the microfluidic system when a microfluidic system is mounted on said platform, d) a control means configured to control relative movement of the one or more actuators, and the microfluidic system when mounted, to enable the magnet to trace a desired path across a mounted microfluidic system, said magnet being positionable at any x- and y-coordinates of the mounted microfluidic system, wherein said apparatus further comprises: a means for moving a mounted microfluidic system in a stepwise fashion.
2. An apparatus according to Claim 1, wherein: i) the mounted microfluidic system is rotatable; optionally ii) the stepwise motion of the mounted microfluidic system may be moveable by non-continuous rotation.
3. An apparatus according to any preceding Claim, wherein i) the one or more actuators further comprise at least one linear actuator; and/or ii) the one or more actuators further comprise at least one linear actuator , and the rotary and linear actuators are on a single driveshaft; and/or iii) the magnet is positionable above a mounted microfluidic system and/or a further magnet of a further one of the one or more actuators is positionable below a mounted microfluidic system, optionally wherein the apparatus comprises a means to move magnet clear of the microfluidic device, and further optionally wherein the means to move a magnet clear of the microfluidic device comprises a rotary actuator configured to allow rotation of the magnetic drive- shaft about an x-axis; and/or iv) the magnet is configured to contact or be held at a fixed distance from the surface of a mounted microfluidic system.
4. An apparatus according to any one of Claims 1 to 3, wherein the magnet is mounted on an adjustable mounting assembly, wherein the adjustable mounting assembly comprises an adjustable magnet holder and a through plate located on a portion of the rotary and/or linear actuator; optionally: i) wherein the through plate comprises a first engagement portion; and wherein the adjustable magnet holder comprises a second engagement portion configured to engage with the first engagement portion of the through plate; and/or ii) wherein the first engagement portion of the through plate comprises a threaded portion and the second engagement portion of the adjustable magnet holder comprises a corresponding threaded portion.
5. An apparatus according to Claims 4, wherein: i) the adjustable mounting assembly is configured to allow positioning of the magnet in a position along a Z-axis; and/or ii) the magnet is configured to be in contact with the surface of the microfluidic device.
6. An apparatus according to any preceding Claim, wherein the platform comprises a turntable configured to receive and controllably rotate an assay disc comprising a microfluidic system, optionally wherein i) the control means controls one or more actuators and the turntable to enable the magnet to trace a desired path across a mounted microfluidic system; and/or ii) the turntable comprises one or more heater modules to apply heat to one or more specific parts of a mounted microfluidic system during rotation.
7. An apparatus according to Claim 6, wherein said turntable further comprises: a heater controller to select a required heater and to control the temperature thereof, an IR transceiver to allow instructions and/or heating parameters to be transferred to the heater controller wirelessly.
8. An apparatus according to any of Claims 6 and 7, wherein said turntable further comprises a means to facilitate the correct positioning of the assay disc relative to the turntable, optionally wherein i) said means comprise a central spindle on the turntable corresponding to a spindle hole in the assay disc, and wherein said spindle has a longitudinal groove to correspond with a longitudinal projection on the hub of the assay disc, optionally wherein the horizontal dimension of the groove of the spindle decreases in a longitudinally downward direction; and/or ii) said means comprise a locating projection on the turntable to mate with a corresponding locating recess on an assay disc, optionally at, adjacent to, or near the periphery of the assay disc.
9. An apparatus according to any of Claims 6 to 8, wherein said turntable further comprises clamping means to secure the assay disc to the turntable, optionally wherein said clamping means comprise a mechanical ball bearing clamping mechanism to hold the assay disc to the turntable.
10. An apparatus according to Claim 9, wherein clamping means comprise magnetic means for holding the assay disc in a fixed position relative to the turntable and/or for aligning and/or guiding the disc to the correct position, optionally wherein said magnetic means comprise disc magnets arranged in opposite pole directions in the turntable corresponding to disc magnets arranged in opposite pole directions in the assay disc.
11. An apparatus according to any of Claims 6 to 10 wherein said turntable comprises a power transfer assembly comprising a wireless power transfer means to transfer power to heater modules in the turntable and/or to the heater controller.
12. An apparatus according to any of Claims 7 to 10, wherein: i) the IR transceiver comprises one or more IR emitters and one IR receiver, optionally wherein the IR transceiver comprises four IR emitters, and further optionally wherein the four emitters are arranged in equidistance surrounding the central hub of the turntable; and/or ii) there are one or more heater modules, optionally a. wherein the heater modules are independently controlled; and/or b. wherein the power supplied to the heater can be shared and distributed among the heaters on demand.
13. An apparatus according to any of Claims 6 to 12, wherein the turntable comprises a power transfer assembly comprising a stationary coil assembly and a rotational coil assembly with the stationary coil assembly mounted axially coincident below.
14. Assay unit comprising an apparatus as claimed in any preceding claim, further comprising means for heating and/or cooling the ambient temperature of the chamber within which the assay disc is located during the assay, and optionally wherein said means is a fan heater or cooler.
15. The combination of an apparatus as claimed in any of Claims 1 to 9 or an assay unit as claimed in Claim 14, and an assay disc comprising a microfluidic system comprising magnetic beads configured to be mounted on the apparatus.
16. Use of an apparatus of Claims 1-13 or an assay unit of Claims 14 or 15 to conduct an assay.
17. A method for carrying out an assay comprising the steps of: i) mounting an assay disc comprising a microfluidic system on a turntable, wherein said microfluidic system comprises a plurality of magnetic beads; and ii) providing a magnet on an actuator such that the magnet may be positioned at any x- and y-coordinates of the assay disc, further comprising the step of moving a plurality of magnetic beads through a portion of the microfluidic system by moving said magnet whilst rotating said assay disc such that said magnet traces a desired locus in an x-, y-plane of the assay disc.
18. An apparatus according to any one of Claims 1 to 13, substantially as herein described with reference to any example thereof.
19. An assay unit according to Claim 14, substantially as herein described with reference to any example thereof.
20. Use according to Claim 16, substantially as herein described with reference to any example thereof.
21. A method according to Claim 17, substantially as herein described with reference to any example thereof.
NZ761782A 2018-07-20 Apparatus for conducting an assay NZ761782B2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1711804.3A GB201711804D0 (en) 2017-07-21 2017-07-21 Apparatus for conducting an assay
PCT/GB2018/052054 WO2019016562A1 (en) 2017-07-21 2018-07-20 Apparatus for conducting an assay

Publications (2)

Publication Number Publication Date
NZ761782A NZ761782A (en) 2024-08-30
NZ761782B2 true NZ761782B2 (en) 2024-12-03

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