TH1863A3 - Process of polyphenol extracts and analysis from Euphoria longana Lam. Sapindaceae fruit for use as raw material for product production. - Google Patents

Abstract

This invention relates to the analysis of active substances from longan fruit. Especially the longan seeds, the preparation of the active ingredients from the longan fruit, the use of the active ingredients from the longan fruit in the production of products. The active ingredients from the longan fruit consist of gallic acid, A. Ellagic acid (ellagic acid) and corilagin (corilagin), or such substances may be in the form of ellagic acid (ellagic acid) combined with gallic acid. Sid (gallic acid) or only chorilagin (corilagin) using the extraction method. Solvent mixed with alcohol and water or hot water only. And measure the amount of such extracts The extracts with the unique analytical techniques described in the above claim were based on High Performance Liquid Chromatography (HPLC) for the selection of raw materials, which were longan seeds. Leftover to produce canned fruit or dried longan Selected longan strains containing substances content Such significance is quite high. Then bring the longan fruit Especially the longan seeds were dried at the appropriate temperature and through the extraction process to obtain polyphenols. (polyphenols) with a higher content of Raw materials that have not been dried Or extraction without going through heat To get the extract Featuring an Ellagik High content of acid The resulting extract can be used as a raw material for the production of pharmaceuticals, supplements, beverages or cosmetics, and preserve food and beverages. By virtue of expanding blood vessels Antioxidant Methicillin-Rcsistant Staphylococcus aureus and MRSA (Methicillin-Rcsistant Staphylococcus aureus)

Claims (9)

1. Preparation method for polyphenol extracts And analyzed the contents of several important substances from longan fruit, including chorilagin, gullic acid and ellagic acid, which are unique in that they are composed by means of extraction and analysis of substances as well. HPLC in one analysis This includes the following steps: a. Fresh and dried longan fruit is divided into parts, peel, pulp and seeds, cut into small pieces or ground into powder and extract each part. Using a solvent mixed with alcohol and water or hot water. B. Remove the residue to be extracted again 1 time using the same solvent. Then be blended and combined. C. All clear extracts in a 10 ml volumetric flask. C. Adjust the volume and then filter them with a 0.45 μm membrane-packed filter unit. D. The substance obtained from the procedure was analyzed using the HPLC at one time. 1.-30 ํ used is 25 ํ. 3. Method according to claim 1 or 2 in which longan fruit is used as dried longan obtained from longan fruit. Freshly baked by heating 60-90 degrees Celsius, suitable at 80 degrees Celsius for 24-72 hours, suitable at 48 hours, or may be baked only from longan seeds that can be selected from the variety. Round red, black leaves, water chestnuts, pink, deformed green and Phet Sakorn 4. Method according to claim 1 or 2 in which the longan is fresh Especially the fresh seeds that can be picked from the line Black leaf varieties, Edo, green, frustration and red round. 5. Method of preparation of products from longan extraction from any of the claims 1 to 3 Which is concentrated or dried by powder freeze-drying, heat or vacuum, with raw material ratio: the extraction solvent is in the range 1: 4-6 weight per volume 6. Use of extracts obtained from any one of Claim No. 1 to 5 in preparation for production. Pharmaceuticals, Food Supplements, Beverages Or cosmetics used in people And / or animals 7. Use of claim No. 6 The drug is used for the treatment of fungal and yeast-related diseases, especially Cryptococus neoformans, which cause brain pathologies. And the cortex by Only for HIV-infected patients, also applicable to Candida grabata, C. parapsillosis and C.albicans that cause oral pathology, etc. 8. Use according to claim 6, which cosmetics are in the form of creams. Or lotion for face or body 9. Use of extracts obtained from claim 3 in conjunction with beta-lactam for the treatment of Infectious type MRSA (Methicillin-Resistant Staphylococcus aureus) by giving the amount of Extract in such products is 0.5-75% of the product.