JP6948833B2 - Maillard reaction product degrading agent - Google Patents

Description

The present disclosure relates to Maillard reaction product degrading agents and beverage compositions, food compositions, functional foods, cosmetics, supplements, quasi-drugs or pharmaceuticals containing them.

It is known that the oxidative reaction and the glycation reaction in the living body adversely affect cells and tissues. For example, a protein such as collagen is saccharified by a glycation reaction (Maillard reaction) in a living body, and amino acids in the protein become AGEs (advanced glycation end products), or a crosslinked structure is formed between proteins by AGEs. In this way, the accumulation of AGEs in proteins due to the Maillard reaction and the formation of cross-linked structures due to AGEs cause diseases such as diabetic complications, Alzheimer's disease, cataracts, and arteriosclerosis, as well as aging and functional deterioration of tissues such as skin. ing.

N-phenacylthiazolium bromide (PTB) and N-phenacyl-4,5-dimethylthiazolium bromide (ALT-711) are known as agents that decompose AGEs (Non-Patent Documents 1 and 2). However, these have not been utilized in terms of stability and side effects. In addition, lotus leaf extract, red pepper extract and the like have been studied (Patent Document 1).

JP-A-2007-119373

Sara Vasan et al. Nature, 382, pp275-278 (1996) Sara Vasan et al. Archives of Biochemistry and Biophysics, 419, 89-96 (2003)

As described above, it is considered that the accumulation of AGEs associated with the Maillard reaction and the crosslinked structure formed accompanying the accumulation can cause various diseases such as the progress of aging and diabetic complications. Therefore, there is a demand for new substances such as AGEs and their intermediates that can decompose the products of the Maillard reaction. Therefore, the present disclosure provides a new Maillard reaction product decomposing agent and a beverage composition, a food composition, a functional food, a cosmetic, a supplement, a quasi-drug or a pharmaceutical product containing the new Maillard reaction product decomposing agent as an active ingredient.

The present disclosure relates, in one aspect, to a Maillard reaction product degrading agent comprising an extract of Fennell as an active ingredient.

The present disclosure, in other aspects, relates to beverage compositions, food compositions, functional foods, cosmetics, supplements, quasi-drugs or pharmaceuticals containing the Maillard reaction product degrading agent of the present disclosure as an active ingredient.

The present disclosure, in other aspects, comprises a Beverage composition, a food composition, a functional food, a cosmetic, a supplement, a quasi-drug for enhancing the Maillard reaction product decomposition activity, which comprises an extract of Fennell as an active ingredient. Or related to pharmaceuticals.

According to the present disclosure, in one embodiment, a new Maillard reaction product decomposing agent, and a beverage composition, a food composition, a functional food, a cosmetic, a supplement, a quasi-drug capable of enhancing the Maillard reaction product decomposing activity. Or can provide medicines.

In the present disclosure, the water extracts of Fennell (scientific name Foenicularum vulgare), lemon balm (scientific name Melissa officeis) and rosemary (scientific name Rosmarinus officeis) are higher than PTB known as a cross-linking decomposition agent by the Maillard reaction. It is based on the new finding that the water extract of Fennell, especially the hot water extract, has the highest resolution of the crosslinked structure by the Maillard reaction.

[Maillard reaction product decomposing agent]
The present disclosure relates to a Maillard reaction product decomposing agent (the Maillard reaction product decomposing agent of the present disclosure) containing an extract of Fennell as an active ingredient as one aspect. The Maillard reaction product-degrading agent of the present disclosure can decompose a product (Maillard reaction product) by the Maillard reaction in one or more embodiments, and preferably an amino group of an amino acid, a peptide or a protein in vivo. It can exert the effect of being able to decompose a product by an aminocarbonyl reaction (also referred to as a Maillard reaction or a saccharification reaction) between a sugar and a carbonyl group derived from a sugar.
According to the Maillard reaction product decomposing agent of the present disclosure, in one or more embodiments, the accumulation of AGEs can be suppressed, and the crosslinked structure of proteins and the like formed by AGEs can be decomposed. As a result, it is possible to prevent, improve, or treat diseases, tissue aging, and tissue dysfunction caused by these. According to the Maillard reaction product degrading agent of the present disclosure, metabolism can be improved in one or more embodiments.
Further, since the Maillard reaction product decomposing agent of the present disclosure uses an extract of Fennell, which is a plant, as an active ingredient, it can have an effect of being excellent in safety and productivity.

In the present disclosure, the "Maillard reaction product" refers to a substance produced by the Maillard reaction. The material produced by the Maillard reaction, in one or more embodiments, comprises an intermediate produced by the Maillard reaction and a final product produced by the Maillard reaction. Examples of the Maillard reaction product include polypeptides and proteins containing AGEs, polypeptides and proteins having a crosslinked structure by AGEs, and glycated proteins produced by the Maillard reaction in one or more embodiments. Examples of the intermediate include substances in which the Maillard reaction is in progress in one or more embodiments, and examples thereof include, but are not limited to, glyoxal, methylglyoxal, 3-deoxyglucosone, and the like. Examples of AGEs include amino acids and dipeptides saccharified by the Maillard reaction in one or more embodiments, and examples thereof are not limited to pentosidine, crosline, pyropyridine, pyrarin, carboxymethyl lysine (CML), and carboxyethyl lysine. , Carboxymethylarginine (CMA), argpyrimidine, imidazolone compound, glyoxal-lysine dimer (GOLD), methyl glyoxal-lysine dimer (MOLD) and the like.

The Maillard reaction product decomposing agent of the present disclosure has the effect of decomposing the Maillard reaction product in one or more embodiments. In the present disclosure, "degrading the Maillard reaction product" means, in one or more embodiments, decomposing intermediates formed by the Maillard reaction, or cross-linking structures between polypeptides and / or proteins by AGEs. Includes cutting. "Degrading the Maillard reaction product" includes, in one or more embodiments, other polypeptides and proteins containing AGEs, polypeptides and proteins having a crosslinked structure by AGEs, or glycation produced by the Maillard reaction. It may include breaking down proteins. Further, in one or a plurality of embodiments without particular limitation, CC of a diketone structure contained in a polypeptide and protein containing AGEs, a polypeptide and protein having a crosslinked structure by AGEs, or a glycated protein produced by the Maillard reaction. It may include breaking the bond.
In addition, the Maillard reaction product decomposing agent of the present disclosure can decompose collagen crosslinks (bad crosslinks or aging crosslinks) formed in collagen molecules by saccharification, oxidation, or the like in one or more embodiments. Thereby, according to the Maillard reaction product decomposing agent of the present disclosure, the accumulation of AGEs in collagen can be suppressed in one or more embodiments.

The Maillard reaction product degrading agent of the present disclosure further comprises at least one extract of lemon balm and rosemary as an active ingredient in one or more embodiments from the viewpoint of further improving the resolution of the crosslinked structure by the Maillard reaction. You may. In addition, the Maillard reaction product decomposing agent of the present disclosure preferably contains at least one of lemon balm and fenugreek from the viewpoint of promoting the decomposition of collagen crosslinks.

The Maillard reaction product degrading agent of the present disclosure may further contain, in one or more embodiments, at least one extract of fenugreek (scientific name: Trigonella foenum-gravecum) and hibiscus (scientific name: Hibiscus sabdarifa) as an active ingredient. ..

When the Maillard reactive decomposition agents of the present disclosure contain extracts of plants other than Fennell, they may be blended in the same ratio (1 part by weight to 1 part by weight of Fennell's extract) or in different ratios. It may be blended in.

The Maillard reaction product degrading agents of the present disclosure include, in one or more embodiments, an extract of Fennell and an extract of lemon balm. In this embodiment, the blending ratio (dry weight ratio) of the phenel extract and the lemon balm extract is 0.1 to 10 parts by weight of the lemon balm extract per 1 part by weight of the phenel extract, and the mailerd reaction. The amount of the lemon balm extract is 0.2 to 5 parts by weight from the viewpoint of further improving the resolution of the crosslinked structure and from the viewpoint of promoting the decomposition of the collagen crosslinked.

The Maillard reaction product degrading agents of the present disclosure include, in one or more embodiments, an extract of Fennell and an extract of rosemary. In this embodiment, the blending ratio (dry weight ratio) of the phenel extract and the rosemary extract is 0.1 to 10 parts by weight of the rosemary extract per 1 part by weight of the phenel extract. From the viewpoint of further improving the resolution of the crosslinked structure by the Maillard reaction, the rosemary extract is 0.2 to 5 parts by weight.

The Maillard reaction product degrading agents of the present disclosure include, in one or more embodiments, an extract of fenugreek and an extract of fenugreek. In this embodiment, the blending ratio (dry weight ratio) of the fenugreek extract and the fenugreek extract is 0.1 to 10 parts by weight of the fenugreek extract per 1 part by weight of the fenugreek extract, and collagen cross-linking. The extract of fenugreek is 0.2 to 5 parts by weight from the viewpoint of accelerating the decomposition of fenugreek.

The Maillard reaction product degrading agents of the present disclosure include, in one or more embodiments, an extract of fenugreek, an extract of lemon balm, an extract of rosemary and an extract of fenugreek. In this embodiment, the blending ratio (dry weight ratio) of the Fennell extract, the lemon balm extract, the rosemary extract, and the fenugreek extract is 0 per part by weight of the Fennell extract. .05 to 20 parts by weight, rosemary extract 0.05 to 20 parts by weight, fenugreek extract 0.05 to 20 parts by weight, from the viewpoint of further improving the resolution of the bridge structure by the mailerd reaction and collagen cross-linking. Lemon balm extract is 0.1 to 10 parts by weight, rosemary extract is 0.1 to 10 parts by weight, and fenugreek extract is 0.1 to 10 parts by weight from the viewpoint of accelerating the decomposition of fenugreek. ..

The extract may be extracted from any part. Examples of the site of use include whole plants, flowers, leaves, seeds, fruits, branches, stems, roots, calyxes, bracts and the like in one or more embodiments. The site to be used may be one type, or two or more types of sites may be used in combination. Fennells are preferably seeds in one or more embodiments. Rosemary is preferably leaves in one or more embodiments. Lemon balm is preferably leaves in one or more embodiments. Fenugreek is preferably seeds in one or more embodiments. The hibiscus is preferably bracts and bracts in one or more embodiments.

The extraction method is not particularly limited, and examples thereof include solvent extraction in one or more embodiments. Examples of the extraction solvent include water, alcohols such as methanol and ethanol, ether and the like in one or more embodiments. Examples of the extract include a water extract, an organic solvent extract, and the like in one or more embodiments. Examples of the water extract include hot water extracts and the like in one or more embodiments. The temperature of the solvent extraction may be 60 to 100 ° C., 70 to 90 ° C., 80 ° C. or the like in one or more embodiments. The solvent extraction time may be 30 minutes to 5 hours, 1 to 5 hours, 2 to 5 hours, 3 to 5 hours, 1 hour, or the like in one or more embodiments. The temperature of hot water extraction may be 60 to 100 ° C., 70 to 90 ° C., 80 ° C. or the like in one or more embodiments. The hot water extraction time may be 30 minutes to 5 hours, 1 to 5 hours, 2 to 5 hours, 3 to 5 hours, 1 hour, or the like in one or more embodiments. The form of the extract includes, in one or more embodiments, an aqueous solution, a concentrate, a dried product, and the like. Drying can be carried out in one or more embodiments by heat-treating the extract obtained by solvent extraction. The heating temperature is 70-150 ° C or 110 ° C in one or more embodiments. The heating time is 1-10 hours or 4 hours in one or more embodiments.
Suitable methods for the production of the Maillard reaction product degrading agent of the present disclosure are as follows in one or more embodiments. An appropriate amount of Fennell powder (for example, 2 g) is extracted with an appropriate amount of distilled water (for example, 40 mL) at 80 ° C. for 1 hour, then cooled to room temperature, and then the obtained slurry is filtered to obtain the obtained filtration. The product is used as a plant extract. When using dried plant extracts, the resulting plant extracts are placed on aluminum trays and dried, for example, in an incubator set at 110 ° C. for 4 hours.

The form of the Maillard reaction product decomposing agent of the present disclosure is not particularly limited, and examples thereof include solids, granules, powders, pastes, and liquids in one or more embodiments.

The Maillard reaction product decomposing agent of the present disclosure may contain other extracts and other components in one or more embodiments as long as it does not interfere with the ability of the active ingredient to decompose AGEs.

The Maillard reaction product decomposing agent of the present disclosure can be used as a raw material for beverage compositions, food compositions, functional foods, cosmetics, supplements, feeds, quasi-drugs, pharmaceuticals, etc. in one or more embodiments. The form as a raw material is not particularly limited, and examples thereof include solids, granules, powders, pastes, and liquids in one or more embodiments.
The present disclosure relates, as one or more embodiments, to beverage compositions, food compositions, functional foods, cosmetics, supplements, feeds, quasi-drugs or pharmaceuticals, including the Maillard reaction product degrading agents of the present disclosure.

The daily intake of the Maillard reaction product decomposing agent of the present disclosure is not particularly limited, and in one or more embodiments, it is 5 mg or more, or 2000 mg or less.

[Manufacturing method of Maillard reaction product decomposing agent]
The present disclosure relates to, in other embodiments, a method for producing a Maillard reaction product degrading agent for decomposing a Maillard reaction product, which comprises solvent extraction of the phenel. According to the production method of the present disclosure, the Maillard reaction product decomposing agent of the present disclosure can be produced.

The method for producing the Maillard reaction product degrading agent of the present disclosure further comprises solvent extraction of at least one plant selected from the group consisting of lemon balm, rosemary, fenugreek and hibiscus in one or more embodiments. You may.

The production method of the present disclosure may include solvent extraction of dried plants in one or more embodiments.

The production method of the present disclosure may include, in one or more embodiments, filtering or centrifuging the extract obtained by solvent extraction.

The production method of the present disclosure may include drying the resulting extract in one or more embodiments.

The production method of the present disclosure may include, in one or more embodiments, mixing an extract of phenel with an extract of a plant other than phenel to obtain a mixture of extracts. The mixing ratio may be the same ratio (1 part by weight with respect to 1 part by weight of the extract of Fennell) or different ratios in one or more embodiments.

The production method of the present disclosure comprises mixing these extracts in one or more embodiments so that the extract of lemon balm is 0.1 to 10 parts by weight per part by weight of the extract of Fennell. However, the resolution of the cross-linked structure by the Maillard reaction can be further improved. From the viewpoint of obtaining a Maillard reaction product decomposing agent and / or a Maillard reaction product decomposing agent capable of promoting the decomposition of collagen cross-linking, an extract of lemon balm is obtained. May include mixing these extracts so that is 0.2-5 parts by weight.

The production method of the present disclosure is to mix these extracts in one or more embodiments so that the rosemary extract is 0.1 to 10 parts by weight per 1 part by weight of the Fennell extract. These extracts may be included so that the Rosemary extract is 0.2 to 5 parts by weight from the viewpoint of producing a Maillard reaction product decomposing agent capable of further improving the resolution of the crosslinked structure by the Maillard reaction. May include mixing.

The production method of the present disclosure comprises mixing these extracts in one or more embodiments so that the extract of fenugreek is 0.1 to 10 parts by weight per part by weight of the extract of fenugreek. However, from the viewpoint of producing a Maillard reaction product decomposing agent capable of accelerating the decomposition of collagen cross-linking, it is included to mix these extracts so that the fenugreek extract is 0.2 to 5 parts by weight. You may be.

The production method of the present disclosure, in one or more embodiments, is 0.05 to 20 parts by weight of lemon balm extract and 0.05 to 20 parts by weight of rosemary extract per 1 part by weight of Fennell's extract. , These extracts may be mixed so that the Fenuglique extract is 0.05 to 20 parts by weight, and the resolution of the crosslinked structure by the Maillard reaction can be further improved. 0.1 parts by weight of lemon balm extract, 0.1 to 10 parts by weight of rosemary extract, and phenuglique from the viewpoint of producing a Maillard reaction product decomposition agent capable of accelerating the decomposition of collagen crosslinks. These extracts may be mixed so that the extracts of the above are 0.1 to 10 parts by weight.

The production method of the present disclosure may include, in one or more embodiments, mixing a phenel with a plant other than the phenel and solvent-extracting the resulting mixture to obtain a mixed extract. The mixing ratio may be the same ratio (1 part by weight with respect to 1 part by weight of Fennell) or different ratios in one or more embodiments. Further, the mixture may be mixed so that the ratio of the extract of each plant in the obtained mixed extract is the same ratio (1 part by weight with respect to 1 part by weight of the extract of Fennell), or the ratio is different. May be mixed with.

[Beverage composition, food composition, functional food, cosmetics, supplements, quasi-drugs or pharmaceutical manufacturing method]
The present disclosure relates to, in other aspects, a method for producing a beverage composition, a food composition, a functional food, a cosmetic, a supplement, a quasi-drug, or a pharmaceutical product (a method for producing the beverage composition, etc. of the present disclosure). The method for producing a beverage composition or the like of the present disclosure is used for a beverage composition, a food composition, a functional food, a cosmetic, a supplement, a quasi-drug, or a pharmaceutical product in order to impart a decomposition effect of the Maillard reaction product. Includes the addition of an extract of.

The method for producing the beverage composition and the like of the present disclosure is further selected from the group consisting of lemon balm, rosemary, fenugreek and hibiscus in order to impart a decomposition effect of the Maillard reaction product in one or more embodiments. It may include adding at least one botanical extract to a beverage composition, food composition, functional food, cosmetics, supplement, quasi-drug or pharmaceutical.

Examples of the extract include a water extract and an organic solvent extract of the plant in one or more embodiments. Examples of the water extract include hot water extracts and the like in one or more embodiments. The form of the extract includes, in one or more embodiments, an aqueous solution, a concentrate, a dried product, and the like.

[Extract composition]
The present disclosure relates to an extraction composition of Fennell for enhancing the decomposition function of the Maillard reaction product in other aspects. In the present disclosure, the "extraction composition" refers to a liquid or solid obtained by extracting a plant. Examples of the extraction composition include a supernatant and a filtrate obtained by solvent-extracting a plant in one or more embodiments. Examples of the extraction solvent include water, alcohols such as methanol and ethanol, ether and the like in one or more embodiments. The form of the extraction composition includes, in one or more embodiments, an aqueous solution, a concentrate, a dried product, and the like. The extract compositions of the present disclosure can be used as food ingredients in one or more embodiments. In the present disclosure, the "food raw material" refers to a raw material or raw material used in the manufacture of foods such as processed foods and health foods and supplements. In one or more embodiments, the form of the food raw material includes an aqueous solution, a concentrated solution, a dried product, and the like.

The extract composition of the present disclosure may comprise, in one or more embodiments, an extract of at least one plant selected from the group consisting of lemon balm, rosemary, fenugreek and hibiscus.

[Maillard reaction product decomposition method]
The present disclosure relates to, in one aspect, a method of degrading a Maillard reaction product comprising administering an extract of Fennell to a living body. The Maillard reaction product degradation method of the present disclosure comprises administering to the living body an extract of at least one plant selected from the group consisting of lemon balm, rosemary, fenugreek and hibiscus in one or more embodiments. It may be included. The present disclosure relates to, in one aspect, a method of degrading a Maillard reaction product comprising feeding a human or non-human organism an extract of Fennell. In one or more embodiments, the Maillard reaction product decomposition method of the present disclosure replaces the above extract with the Maillard reaction product decomposition agent, beverage composition, food composition, functional food, cosmetics, etc. of the present disclosure. Supplements, non-pharmaceutical products or pharmaceuticals may be used.

The present disclosure may relate to one or more of the following embodiments:
[1] A Maillard reaction product decomposing agent containing an extract of Fennell as an active ingredient.
[2] The Maillard reaction product decomposing agent according to [1], which further comprises an extract of at least one of lemon balm and rosemary.
[3] The Maillard reaction product decomposing agent according to [1] or 2, wherein the extract is at least one of a water extract and an organic solvent extract.
[4] The Maillard reaction product decomposing agent according to any one of [1] to [3], which comprises a dried product of the extract as an active ingredient.
[5] Beverage compositions, food compositions, functional foods, cosmetics, supplements, quasi-drugs or pharmaceuticals containing the Maillard reaction product decomposing agent according to any one of [1] to [4] as an active ingredient. ..
[6] Beverage compositions, food compositions, functional foods, cosmetics, supplements, quasi-drugs or pharmaceuticals for enhancing the decomposition activity of Maillard reaction products containing an extract of Fennell as an active ingredient.
[7] A method for producing a Maillard reaction product decomposing agent, which comprises extracting a phenel with a solvent.
[8] Including adding an extract of Fennell to a beverage composition, a food composition, a functional food, a cosmetic, a supplement, a non-pharmaceutical product or a pharmaceutical product in order to impart a decomposition effect of the Maillard reaction product. , Beverage composition, food composition, functional food, cosmetics, supplements, non-pharmaceutical products or pharmaceutical manufacturing methods.

The present disclosure will be further described below with reference to examples. However, this disclosure is not construed as limited to the following examples.

[Preparation of plant extract]
2 g of the dry powder of the plant shown in Table 1 below was extracted with 40 ml of distilled water at 80 ° C. for 1 hour, cooled to room temperature and filtered, and the obtained filtrate was used as a plant extract.
The solid content concentration of each sample was calculated by placing 5 mL of the obtained filtrate in an aluminum tray and drying it in an incubator set at 110 ° C. for 4 hours, and then measuring the residual amount. The results are shown in Table 1 below.

[AGEs Crosslink Cutting Action Confirmation Test 1]
The procedure was performed according to the description of Sara Vasan et al. Nature, 382, pp275-278 (1996).
As a substrate, 1-Phenyl-1,2-propanedione (PPD), which is a reaction substrate of an AGEs cross-linking model having an α-diketone structure, was used, and as a positive control, N-phenacylthiazolium bromide (PTB) was used.
The plant extract, 10 mmol / L PPD, and 0.2 mol / L phosphate buffer (pH 7.4) were mixed at a ratio of 5: 1: 4 and reacted at 37 ° C. for 8 hours (n = 3). After completion of the reaction, hydrochloric acid was added to stop the reaction.
The reaction mixture was centrifuged at 3,000 × g at 20 ° C. for 10 minutes, and the amount of benzoic acid in the supernatant was analyzed by reverse phase HPLC. The amount of benzoic acid in the reaction solution was determined by subtracting the amount of benzoic acid in the separately measured sample. Since 1 mol of PPD produces 1 mol of benzoic acid, the cross-linking cleavage rate was calculated from the following formula. The results are shown in Table 1 below.
Crosslink cutting rate (%) = {(AB) / C} x 100
A: Amount of benzoic acid in the reaction solution B: Amount of benzoic acid in the sample C: Amount of PPPD used in the reaction (base mass)

As a control, 10 mmol / L PTB was used instead of the plant extract.

As shown in Table 1, all of Fennell, Lemon Balm and Rosemary showed higher cross-linking rate than PTB reported as a compound that decomposes cross-linking structures involving AGEs. In particular, Fennell showed a cross-linking cleavage rate nearly twice that of PTB.

[AGEs Crosslink Cutting Action Confirmation Test 2]
The procedure was performed according to the description of Sara Vasan et al. Nature, 382, pp275-278 (1996).
SuperBlock T20 (PBS) Blocking Buffer was added to a commercially available 96-well microplate coated with collagen at 300 μL / well and reacted at 37 ° C. for 1 hour. After the reaction, the cells were washed with 0.05% Polyethylene (20) sorbitan monolaurate (Tween 20) / PBS (-), and then pre-AGEs bovine serum albumin (AGE-BSA) was added to each well of the microplate at 37 ° C. The cross-linking reaction was carried out for 4 hours. After the reaction, the cells were washed with Tween 20 / PBS (-), and then a plant extract was added to each well, and the cleavage reaction was carried out at 37 ° C. for 18 hours. Then, it was washed with Tween 20 / PBS (-), a primary antibody (anti-albumin bovine serum rabbit-polyclonal) was added, and the mixture was reacted at room temperature for 30 minutes. Furthermore, after washing with Tween 20 / PBS (-), a secondary antibody (goat anti-rabbit IgG horseradish peroxidase (HRP) conjugate) was added and reacted at room temperature for 30 minutes. After that, it was washed with Tween 20 / PBS (-), and TMB One Component HRP Microwell Substrate was added. Further, a PEG6000 solution was added and the reaction was carried out for 20 minutes. 1N HCl was added as a reaction terminator, and the absorbance at 450 nm (main wavelength) / 630 nm (secondary wavelength) was measured with a microplate reader. The residual amount of AGE-BSA was determined from the calibration curve in which the amount of AGE-BSA was changed. The AGE-BSA decomposition rate (collagen cross-linking decomposition rate) was calculated using the following formula. The results are shown in Table 2 below.
AGE-BSA decomposition rate = {(added AGE-BSA amount-residual AGE-BSA amount) / added AGE-BSA amount} x 100

As a positive control, a similar test was conducted using punicalagin, which is known to have an effect of inhibiting the Maillard reaction activity, instead of the plant extract.

As shown in Table 2, it was confirmed that fenugreek, lemon balm and fenugreek had collagen cross-linking degrading activity. In particular, it was confirmed that lemon balm has collagen cross-linking decomposition activity equivalent to that of punical agin.

Claims (9)

Extract Fennell, extracts of lemon balm, seen containing an extract of rosemary and fenugreek extract as an active ingredient,
The content of the lemon balm extract is 0.05 to 20 parts by weight per 1 part by weight of the extract of the phenel.
The content of the rosemary extract is 0.05 to 20 parts by weight per 1 part by weight of the extract of the phenel.
The fenugreek extract is 0.05 to 20 parts by weight per part by weight of the fenugreek extract.
A Maillard reaction product degrading agent for cleaving the cross-linked structure between polypeptides and / or proteins by AGEs. The content of the lemon balm extract is 0.1 to 10 parts by weight per 1 part by weight of the extract of the phenel.
The content of the rosemary extract is 0.1 to 10 parts by weight per 1 part by weight of the extract of the phenel.
The Maillard reaction product decomposing agent according to claim 1, wherein the extract of fenugreek is 0.1 to 10 parts by weight per 1 part by weight of the extract of fenugreek. Beverage composition and food composition for enhancing the cross-linking activity of AGEs that cleaves the cross-linked structure between polypeptides and / or proteins by AGEs, which comprises the Maillard reaction product-degrading agent according to claim 1 or 2 as an active ingredient. Goods, functional foods, cosmetics, supplements, non-pharmaceutical products or pharmaceuticals. The method for producing a Maillard reaction product decomposing agent according to claim 1 or 2.
A production method comprising solvent extraction of fenugreek, lemon balm, rosemary and fenugreek. The production method according to claim 4 , wherein the solvent is at least one of water and an organic solvent. The production method according to claim 4 or 5 , which comprises drying the extract obtained by the solvent extraction. To impart AGEs crosslinking degradation effect of cutting the crosslinked structure between the polypeptides and / or proteins by AGEs, beverage composition, food composition, functional food, cosmetics, supplements, quasi drugs or the pharmaceutical product, wherein A method for producing a beverage composition, a food composition, a functional food, a cosmetic, a supplement, a quasi-drug, or a pharmaceutical, which comprises adding the Maillard reaction product decomposing agent according to Item 1 or 2. Beverage composition, food composition, functional food, cosmetics, supplements, quasi-drugs, or methods for manufacturing pharmaceuticals.
Fenugreek in beverage compositions, food compositions, functional foods, cosmetics, supplements, non-pharmaceutical products or pharmaceuticals to impart the AGEs cross-linking degradation effect that cleaves the cross-linked structure between polypeptides and / or proteins by AGEs. A production method comprising adding an extract of syrup, an extract of lemon balm, an extract of rosemary and an extract of fenugreek as follows.
Content of the lemon balm extract: 0.05 to 20 parts by weight per part by weight of the extract of the phenell.
Content of the rosemary extract: 0.05 to 20 parts by weight per part by weight of the extract of the phenell.
Extract of fenugreek: 0.05 to 20 parts by weight per part by weight of the extract of fenugreek. Beverage composition according to claim 3, wherein, the food composition, functional food, cosmetics, supplements, for the manufacture of a quasi-drug or pharmaceutical method according to claim 7 or 8.