CN2434678Y - Bacterial endotoxin analyzer - Google Patents

Abstract

A bacterial endotoxin tester is composed of a shell, a single-chip microcomputer arranged on a bottom plate, an integrated circuit, a temperature control device, an electric control device and related circuits; a reactor arranged on the bottom plate, and a photoelectric detection device installed on the reactor Each set of photodetectors is equipped with a blue light-emitting diode and a silicon blue light battery, and the front end of the blue light-emitting diode is provided with a collimation hole; it can realize dynamic turbidimetry and dynamic colorimetry at the same time The method combined with the gel limit method for the quantitative determination of bacterial endotoxin can be applied to the quantitative determination of bacterial endotoxin in medicines, food and various body fluids of the human body.

Description

Bacterial endotoxin analyzer

The utility model relates to a bacterial endotoxin measuring instrument for quantitative determination of bacterial endotoxin in various body fluids of medicines, medical appliances, water, food and beverage, clinical examination and human body through agglutination reaction between Limulus reagent and trace bacterial endotoxin.

Endotoxin is a lipopolysaccharide in the cell wall structure of Gram-negative bacteria. This lipopolysaccharide is synthesized by the bacterial cell wall and then transported to the cell surface to form a component of the outer membrane of the cell wall. After the bacteria dies and dissolves, it is released from the cell structure released in. On the one hand, endotoxin has multiple toxic effects on the host, and on the other hand, it can exert many beneficial biological activities on the body. The quantitative determination of bacterial endotoxin has important guiding significance for pharmaceutical production, clinical testing and food testing.

The quantitative determination of bacterial endotoxin can be divided into gel method, turbidimetric method, colorimetric method and ELISA (enzyme-linked immunoassay) method. Received: The commonly used bacterial endotoxin analyzer, its determination principle is based on the first logarithmic value of the time required for the turbidity change (transmitted light ratio) value of the reaction solution of endotoxin and Limulus reagent and the relationship between the concentration of bacterial endotoxin The logarithmic values are inversely proportional and quantitative.

A general bacterial endotoxin tester is composed of an electric control part, a temperature control part, a reactor part, a photoelectric detection part, etc. Usually, a tester can only use a dynamic turbidimetric method for quantitative determination of endotoxin. When the dynamic colorimetric method is used for measurement, another measuring instrument must be used, and the photoelectric detection part must use light with a wavelength centered at 405nm and a very narrow range. To obtain light with such a narrow range and such a wavelength, existing domestic and foreign Some measuring instruments can only be obtained by using optical filters or gratings. This structure is not only costly, but also difficult to obtain any number of light rays with a wavelength of 405nm; Realize the quantitative determination of endotoxin by dynamic colorimetry. This structure cannot realize the quantitative determination of dynamic turbidimetry, dynamic colorimetry and gel limit method on the same measuring instrument at the same time, and cannot realize rapid bacterial Quantitative determination of endotoxin requires a long detection period, and due to the large aperture of the optical path, the ratio of diameter to length is greater than 1, and the distance between the axis of the optical path and the bottom surface of the test tube hole is relatively large, generally greater than 5mm, which affects the inspection range of the optical path.

The purpose of the utility model is to provide a short detection period, a wide inspection range, which can realize fast dynamic turbidimetry quantitative determination, can obtain multi-channel light inspection range, and can simultaneously realize dynamic turbidimetry, dynamic colorimetry and gel Bacterial endotoxin analyzer for the quantitative determination of bacterial endotoxin by three methods of limited method.

The purpose of this utility model is achieved in that:

A bacterial endotoxin tester is composed of a shell, a single-chip microcomputer arranged on a bottom plate, an integrated circuit, a temperature control device, an electric control device and related circuits; a reactor arranged on the bottom plate, a photoelectric detection device installed on the reactor It is composed of detector, heater and constant temperature block, and each set of photodetectors is equipped with blue light-emitting diodes. The front end of the blue light-emitting diode is provided with a collimation hole. The diameter-to-length ratio of the collimating holes is less than one.

The utility model improves the structure of the photoelectric detection part of the existing endotoxin measuring instrument, is provided with a collimation hole and is equipped with a blue light-emitting diode and a silicon blue light battery, can obtain light of any multiple wavelengths, expand the light inspection range, and can simultaneously Realize dynamic turbidimetric method, dynamic colorimetric method and gel limit method combined quantitative determination of bacterial endotoxin, the utility model is easy to operate, short detection cycle, fast and accurate inspection, can be completed in 30-40 minutes, wide inspection range , from 0.001 to 1000EU/ml, with a wide range of applications. Quantitative detection can monitor the change trend of endotoxin content in the process of pharmaceutical production, which is convenient for manufacturers to take timely measures to avoid economic losses caused by unqualified products. At the same time, quantitative detection is important for many It is of great significance for the diagnosis of diseases and the judgment of treatment effects, especially suitable for the quantitative determination of endotoxin levels in various body fluids of the human body.

Description of drawings

Fig. 1 is the structural representation of the utility model;

Fig. 2 is A-A sectional view among Fig. 1;

Fig. 3 is a sectional view of B direction in Fig. 2;

Fig. 4 is a sectional view along direction C in Fig. 2;

Fig. 5 is a partial schematic diagram of the circuit board structure of the light source of the present invention;

Fig. 6 is a partial schematic diagram of the structure of the photoelectric signal receiving board of the present invention.

Below in conjunction with accompanying drawing and preferred embodiment the utility model is further described:

Referring to Figure 1, the bacterial endotoxin analyzer is mainly composed of a shell 1, a reactor 2, a panel 4, an indicator light 5, a test lamp 6, a test tube hole 7, etc. from the outside, and the reactor 2 is provided with several A test tube hole 7, each test tube hole 7 corresponds to a test lamp 6, can insert a standard test tube 3 in the test tube hole 7, the panel 4 is provided with a power supply indicator light and a temperature control indicator light 5, and the shell 1 is connected with the base plate.

Referring to Fig. 2, the shell 1 is removed in the figure, the bacterial endotoxin tester is mainly composed of a photoelectric signal receiving board 8, a photodetector 9, a heater and a constant temperature block 10, a light source circuit board 12 and a base plate from the internal structure 11, and its single-chip microcomputer, integrated circuit, temperature control device, electric control device and related circuits (not shown in the figure) are prior art, and are arranged on the bottom plate 11, and several sets of photoelectric detection devices are installed on the reactor 2. The reactor 9 and the reactor 2 are also equipped with a heater, a thermostatic block 10 and a temperature sensor constituting a constant temperature function, which are connected as a whole by screws and fixedly connected on the bottom plate 11.

Referring to Fig. 3 and shown in Fig. 4 at the same time, the optical path inner hole of each cover photodetector 9 is equipped with a blue light-emitting diode 14 of 350-550nm wavelength and a corresponding shallow junction silicon blue light cell 16, silicon blue light The P-N junction depth of the battery 16 is less than 100 μm, the photodetector 9 is also equipped with a microswitch 13, the legs of the microswitch 13 and the legs of the blue light-emitting diode 14 are welded to the light source circuit board 12 on one side of the photodetector 9 , the front end of the blue LED 14 is the collimating hole 15 of the optical path, the ratio of the diameter of the collimating hole 15 to the length is less than 1, the collimating hole 15 communicates with the test tube hole 7, and the distance between the axis of the inner hole of the optical path and the bottom surface of the test tube hole 7 Less than 5mm, the upper end of the photodetector 9 is provided with an indicator hole, and a green light-emitting diode 17 is housed in the hole, and its pins and the positive and negative poles of the silicon blue light battery 16 are welded to the photoelectric signal receiving plate 8 on the other side of the photodetector 9 .

Referring to Fig. 5, the light source circuit board 12 is connected to one side of the photodetector 9, and several light source circuit boards 12 are arranged in sequence, such as eight blue light-emitting diodes 14 and four resistors 19, eight groups of which can be inserted into micro-movement The legs of the switch 13 and the two ends of the light source circuit board 12 are provided with connection positions 18, which are connected with the electric control part, the temperature control part and related circuits, single-chip microcomputers, integrated circuits, etc. through flat connections.

Referring to shown in Figure 6, the photoelectric signal receiving board 8 is connected to the other side of the photodetector 9, and several photoelectric signal receiving boards are arranged successively on the photoelectric signal receiving board 8, such as eight silicon blue light cells 16 and eight resistors 19, the photoelectric signal receiving board The two ends of the 8 are provided with connection positions 18, which are connected with the electric control part, the temperature control part and related circuits, single-chip microcomputers, integrated circuits, etc. through flat connections.

The appearance of the utility model, the shape of the reactor, the number of test tube holes and the arrangement can be freely designed according to the needs, and are not limited by the embodiment of the utility model. The utility model aims to protect the improved structure of the photoelectric detection part.

Working principle of the utility model: the measuring principle of the bacterial endotoxin measuring instrument is based on the first-order logarithmic value of the time required for the turbidity change (ratio of transmitted light) of the reaction solution of endotoxin and Limulus reagent and the correlation of the bacterial endotoxin concentration. The values are quantified in an inverse proportional relationship. The utility model carries out data acquisition, temperature control and communication of the host computer through the single chip microcomputer, and the host computer calculates and processes the data, and the specific working process is as follows:

1. Connect the bacterial endotoxin tester and the computer with a serial cable, turn on the power switch 5 of the tester, and turn on the power switch of the computer;

2. The computer enters the WIN95/WIN98 system and runs the bacterial endotoxin determination software;

3. Press the acquisition button in the software program to enter the acquisition state;

4. Thirty minutes after the power switch of the tester is turned on, the closed-circuit control circuit formed by the heater, the constant temperature block 10 and the temperature sensor will accurately control the constant temperature block of the tester within the range of 37 degrees plus or minus 1 degree under the control of the single-chip microcomputer ;

5. Insert the sample test tube 3 of the mixed LAL reagent into the test tube hole 7. At this moment, the microswitch 13 sends a signal, and the single-chip microcomputer performs timing and data collection successively to the holes inserted into the test tube;

6. The blue light-emitting diode 14 emits a beam of light, which passes through the mixed solution in the test tube and hits the photocell 16. The light signal is converted into an electrical signal, which is converted into a digital signal by a single-chip microcomputer and sent to the upper computer;

7. The single-chip microcomputer in the measuring instrument sends a handshake signal to the host computer, and transmits the collected data to the host computer every 10 seconds;

8. The upper computer calculates the received data and draws the S-curve of each test tube hole;

9. The computer gives an alarm to the test tube hole 7 whose reaction time reaches 60 minutes, and the test tube 3 can be pulled out and turned vertically 180 degrees for gel observation, and compared with the quantitative result; after all the test tubes 3 reach 60 minutes, the computer and measuring instrument The collection is automatically terminated, and the calculated quantitative results can be output through the computer;

10. The shutdown is over.

Claims (3)

1. A bacterial endotoxin tester is composed of a shell (1), a single-chip microcomputer arranged on a base plate (11), an integrated circuit, a temperature control device, an electric control device and related circuits thereof; The reactor (2), the photoelectric detector (9) installed on the reactor (2), the heater and the constant temperature block (10) are composed, and it is characterized in that blue light-emitting diodes are housed in each set of photodetectors (9) (14). 2. The bacterial endotoxin analyzer according to claim 1, characterized in that a collimation hole (15) is provided at the front end of the blue light-emitting diode (14). 3. The bacterial endotoxin analyzer according to claim 1 or 2, characterized in that the ratio of the diameter to the length of the collimation hole (15) is less than 1.

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