JP2511818B2 - Skin cosmetics - Google Patents

Description

TECHNICAL FIELD The present invention relates to a skin aging-preventing effect (rough skin improving effect, keratin improving effect, horny layer turnover) obtained by blending a tea leaf extract preserved under anaerobic conditions. The effect of accelerating the skin tone, the effect of improving the beautiful skin, etc.)

(Prior Art) Aging skin is dry and rough skin that is exfoliated by keratinocytes and the connective tissue is collagen /
High elastin ratio and many wrinkles.

It is said that aging skin has a slow turnover of the stratum corneum due to a decrease in cell metabolism, and therefore, when the antiaging effect is imparted to the skin, the turnover is accelerated, and various skin cell activating components and blood circulation promoting components have been added. Cosmetics have been proposed, but none of them have the effect of imparting the skin anti-aging effect to the extent expected by the present invention.

In addition, the inventors of the present invention disclosed in Japanese Patent Publication No. 58-26726 gamma-
Although a cosmetic composition containing aminobutyric acid has been proposed, its skin aging-preventing effect is not sufficiently satisfactory due to its delayed effect.

(Disclosure of the Invention) Therefore, in view of the above-mentioned present situation, the present inventors have conducted extensive studies, and as a result, preserved under anaerobic conditions, heat-treated, and tea leaves were extracted with a solvent described below. Alternatively, a skin cosmetic containing a solid product produced by further concentrating the same promotes blood circulation, which is the purpose of the present invention, activates skin cells, accelerates turnover of aged skin, and improves rough skin. It has a keratin-improving effect, and is moisturizing (moisturizing), flexibility (smoothness), elasticity (tension) on the skin.
Further, they have found that they also exhibit a beautiful skin effect capable of imparting luster, and completed the present invention.

(Object of the invention) That is, an object of the present invention is to provide a skin cosmetic excellent in rough skin improving effect, keratin improving effect, effect of accelerating turnover of the stratum corneum, and skin aging preventing effects such as skin beautifying effect. It is in.

(Structure of the invention) The present invention is to extract tea leaves obtained by storing fresh tea leaves under anaerobic conditions and then subjecting them to steam heat treatment and / or dry heat treatment, and blending 0.1% by weight or more as solid content thereof. It is a skin cosmetic characterized by the following.

(Detailed Description of the Invention) Tea is not only a beverage of choice, but is widely consumed as a health drink due to the physiological actions of vitamin C, caffeine, tannin, amino acids and the like contained in tea.

Various methods have been used for producing tea leaves by various countries since ancient times, but in general, fermenting methods such as gyokuro, matcha tea, sencha, and bancha, semi-fermentation methods such as oolong tea, and fermentation methods such as black tea. Can be separated into All of the operations in the manufacturing process of each of these teas are performed in the presence of oxygen.

However, recently, a method for producing tea having an enhanced blood pressure lowering effect has attracted attention. This method is generally used in the fermentation process of green tea, matcha tea, etc. In the process of manufacturing tea leaves, as a pretreatment for steaming the tea leaves, it is sealed and stored for 5 to 30 hours in a closed container in which air is completely replaced with nitrogen or carbon dioxide. In the fermentation method for black tea or the like, as a pretreatment for withering and kneading tea leaves, the tea leaves are sealed and stored in a closed container similarly replaced with nitrogen or carbon dioxide. The content of γ-aminobutyric acid in the tea leaf extract that has been subjected to the above-mentioned treatment is a maximum of 300 mg / 100 g, whereas in the tea leaf extract that is not treated as described above, 30 mg / 100 g, about 1
Only 1/0 is included. Therefore, the tea leaf extract treated by the conventional manufacturing method hardly exhibits the hypotensive effect,
The tea leaf extract preserved under anaerobic conditions has the advantage of exhibiting an excellent blood pressure lowering action.

The inventors of the present invention sufficiently replace the air in the container containing the plucked green tea leaves with an inert gas such as carbon dioxide gas or nitrogen gas, and then seal the container, for example, at room temperature to 3
Leave for more than an hour (tea leaves are stored under anaerobic conditions)
By paying attention to the phenomenon that the content of γ-aminobutyric acid remarkably increases, as a result of intensive research, as compared with a skin cosmetic containing γ-aminobutyric acid alone, tea leaves preserved under anaerobic conditions Is steam-heated for 10 to 30 minutes, and then naturally dried, or tea leaves dried by being placed in a dryer at 80 ° C. or higher for 5 hours or longer are treated with water, a water-miscible organic liquid,
It is immersed in a solvent such as a mixture of water and a water-miscible organic liquid, and extraction is carried out, for example, at the boiling point of the solvent for 30 to 60 minutes. The extract is concentrated by a usual method or, if necessary, purified by activated clay, chromatography or the like and then concentrated under reduced pressure to obtain a solid (active ingredient).

The extract (solid matter) thus obtained has a large content of γ-aminobutyric acid, and the skin cosmetic composition of the present invention containing this is combined with a skin cell activating action and a blood circulation promoting action, Theophylline having a peripheral vasodilatory action contained in the extract, caffeine having a peripheral nerve stimulating action, flavonoids having a peripheral vascular strengthening and anti-inflammatory action activate skin cells by acting synergistically, Remarkably develops an immediate effect of preventing skin aging, and aspartic acid, glutamic acid, alanine, serine, glutamine,
The moisturizing action of amino acids such as arginine causes the stratum corneum to retain an appropriate amount of water and also imparts a skin-beautifying effect, and the action and effect are remarkable.

Further, the storage time of tea leaves under anaerobic conditions is usually 3 hours or longer, preferably 5 hours or longer, and more preferably 8 hours or longer at room temperature. If the storage time is short, the production of γ-aminobutyric acid and the like is not sufficient, and the desired effect of preventing skin aging is difficult to manifest.

As the water-miscible organic liquid (hydrophilic organic liquid) in the above-mentioned solvent, for example, glycols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, diethylene glycol, glycerin, maltitol, etc. Polyhydric alcohols, ethyl alcohol,
Examples thereof include lower monohydric alcohols such as propyl alcohol and butyl alcohol, water-miscible ketones such as acetone and methyl ethyl ketone, and tetrohydrofuran.

The tea leaves used in the present invention can be applied to any variety such as Yabukita and Beni Fuji, and the plucking time is not particularly limited.

The content of the tea leaf extract obtained by performing the above-mentioned treatment in the present invention is 0.1% by weight (hereinafter abbreviated as wt%) or more based on the total amount of the cosmetic as a solid component thereof. , Preferably 1 to 20 wt%. If it is less than 0.1 wt%, the anti-aging effect of skin targeted by the present invention does not appear as expected, and if it exceeds 20 wt%, the effect commensurate with the increase cannot be expected, which is economically undesirable. .

In the skin cosmetic of the present invention, various oil agents other than the above components,
Alcohols, UV absorbers, pigments, fragrances, preservatives, surfactants, pigments, water-soluble polymers, skin nutrients, antioxidants, moisturizers, thickeners, resin film forming agents, pH regulators, etc. It can be appropriately blended within a range that achieves the object of the present invention.

Further, the dosage form of the skin cosmetic of the present invention can be arbitrarily prepared such as skin lotions, emulsions, creams, packs and foundations.

(Example) Hereinafter, the present invention will be described in detail based on Examples and Comparative Examples.

[Production method of tea leaf extract treated under anaerobic conditions]

Put 500 g of freshly picked tea leaves into a desiccator for vacuum containing 2 liters, put a porous plate made of porcelain on it, seal it with a lid greased with green, connect it to a vacuum pump and evacuate the container Then, it is connected to a nitrogen cylinder to allow nitrogen to flow into the container. When the pressure in the container reaches 0.8 atm, the nitrogen flow is stopped, and the container is left at room temperature of 25 ° C for about 10 hours. After that, the tea leaves treated under anaerobic conditions are taken out of the container, put in a steamer and steamed with steam for 30 minutes, spread on a dry towel, and left to stand in a room at 25 ° C for 2 days to dry naturally. 100 g of the tea leaves thus obtained was placed in a beaker containing 500 ml, and water at 25 ° C or a 10% ethyl alcohol aqueous solution was added.
After injecting 300 ml and heating for 30 minutes, it was separated by filtration to obtain 300 ml of an extract that could be dropped. Next, the extract was concentrated by a commonly used method such as concentration under reduced pressure to obtain 4.6 g of a solid component. Each test sample was prepared by blending this solid component with cosmetics.

The results of analysis of the extract obtained by the present production method are shown in the table below together with the content of the tea leaves ingredient (converted from the content of the extract).

Further, the rough skin improving effect test, the keratin improving test, the stratum corneum turnover measurement test, and the sensory test (beautiful skin effect test) used for evaluating the skin aging preventing effect of the skin cosmetic of the present invention are as follows. .

(1) Rough skin improving effect test 20 middle-aged and elderly subjects having rough skin on the lower legs were tested for the effect of continuous application for 4 weeks. About 1 g of the sample was applied to the left lower leg test site of the test subject once a day, and the condition of the skin before and after the start of the test was evaluated according to the following criteria. The lower right leg was not coated with the sample and served as a control.

Criteria for skin dryness −: Normal ±: Minor dryness, no desquamation +: Dry, mild desquamation ++: Dry, moderate desquamation +++: Dryness, remarkable desquamation Compare the determination results of the test site before and after the test and the control site,
When the skin dryness is improved by two or more steps (for example, + →
−, ++ → ±) is valid, 1 is slightly improved when it is improved, and is invalid when there is no change. The test results were shown by the number of subjects who were valid or slightly valid.

(2) Keratin improvement (increased anti-peeling property of keratinocytes) effect test Scotch tape (Nichiban Mending Tape) is adhered to the skin of the subject before and after the above-described rough skin improvement effect test, and the tape is peeled off. The state of the keratinocytes adhered to was examined in detail by a scanning electron microscope, and the skin keratinocyte anti-exfoliation property was analyzed according to the following criteria to determine the keratin improving effect.

Evaluation criteria for keratin improving effect (increased keratinocyte anti-exfoliation) Evaluation point 1: No scale is observed 2: Small scale is scattered 3: Small to medium scale is remarkable 4: Large scale is remarkable Evaluation is a test after continuous application for 4 weeks When the difference between the evaluation score of the site and that of the control site was 2 or more, the score was valid, 1 score was slightly valid, and 0 score was invalid. The judgment results are shown by the number of subjects who were valid and slightly valid.

(3) Turnover measurement test of stratum corneum 5 g of dansyl chloride, a fluorescent dye, in white petrolatum
Make an ointment containing 20% by weight and apply it to the skin of the forearm of 20 test subjects for 24 hours, and dansyl chloride is permeated and bonded to the stratum corneum. After that, the test sample was applied to the same site twice a day (morning and evening), the fluorescence of dansyl chloride was examined every day, and the number of days until the fluorescence disappeared was defined as the turnover of the stratum corneum of the skin.

The judgment results are shown as the average number of days of the subjects. The normal turnover of the stratum corneum is 14 to 16 days, but in aged skin it extends around 18 days. On the other hand, when the anti-aging effect appears, it is shortened to around 12 days.

(4) Sensory test (Beautiful skin effect test) Female subjects who complain of rough skin, fine wrinkles, dry skin (35-55)
20 years old, the effect of the sample was evaluated twice a day (morning and evening) after 3 consecutive months. The test results were expressed by the number of people who answered that the skin was moistened, the skin became smooth, and the skin became taut for each item of skin wettability, smoothness, and elasticity. Comparative Examples 1 to 4 and Examples 1 to 3 [Two-layer type skin lotion] Purified water extract of tea leaves (hereinafter referred to as A-1) treated with a two-layer type skin lotion base under anaerobic conditions as shown below.
A tea leaf 10% ethyl alcohol aqueous solution extract (hereinafter abbreviated as A-2) and untreated tea leaf extract (hereinafter abbreviated as B-1), respectively, as described in Table 1. Each sample was prepared and used for each test.

(1) Composition (2) Preparation method The tea leaf extract of the component (C) is mixed and dissolved in the component (B) in advance, and then the components (A) and (B) are mixed and stirred to disperse,
Then, the container is filled. At the time of use, the contents should be evenly dispersed by shaking.

(3) Properties The results of various tests of each two-layer type skin lotion are shown in the right column of Table 1.

As compared with the untreated tea leaf extract cosmetics of Comparative Examples 1 to 4, the skin cosmetics of the present invention of Examples 1 to 3 showed good results over all the tests. Examples 4 to 6 and Comparative Examples 5 to 7 [Skin cream] Similar to Example 1, each skin cream was prepared with the following composition and various tests were carried out. The results are shown in the right column of Table 1. .

(1) Composition (2) Preparation method The tea leaf extract of the component (C) is mixed and dissolved in the component (B), and then the components (A) and (B) are dissolved by heating at 80 ° C., and then mixed and stirred. Each skin cream was prepared by cooling to 30 ° C.

(3) Characteristics As shown in Table 1, the skin creams of Examples 4 to 6, which are the skin cosmetics of the present invention, are superior in all of the characteristics as compared with Comparative Examples 5 to 7. Obviously, no abnormality was found in the compounding characteristics.

(Effect of the Invention) As described above, it is apparent that the skin cosmetic of the present invention provides an excellent skin cosmetic that enhances the skin function and exerts a remarkable effect in preventing skin aging.

Claims (1)

(57) [Claims] 1. A tea leaf extract obtained by storing fresh tea leaves under anaerobic conditions, followed by steam heat treatment and / or dry heat treatment, in an amount of 0.1% by weight or more as a solid content. Skin cosmetics.