JP2618658B2 - Skin cosmetics - Google Patents

Description

The present invention relates to new skin cosmetics (including quasi-drug cosmeceuticals and medicinal dermatological preparations) which are effective in normalizing human skin. The same), that is, a microbial preparation effective for the prevention or treatment of spots, freckles, and so on.

More specifically, one or more of Rhizopus oligosporus, Rhizopus oryzae, Rhizopus arrhizus or Rhizopus stolonifer in a soybean or a medium containing soybeans The present invention relates to a skin cosmetic containing, as an active ingredient, a fermentation product obtained by reacting the same.

(Prior art and its problems) Conventionally, as a skin cosmetic, it has been proposed to mix various drugs for the prevention or treatment of spots, freckles, etc., but its efficacy, safety or stability From the point of view, few are satisfactory.

In recent years, vitamin C or its derivatives, aloe extract, placenta extract, glutathione, etc. have been used for this purpose, but are not yet satisfactory. That is, vitamin Cs, placenta extract, and glutathione have a problem in stability after preparation, and aloe extract has a defect of browning over time.

(Progress of the Invention) The present inventor has conducted intensive studies to develop a truly effective skin cosmetic, and as a result, found that soybeans or a medium containing soybeans could be used in Rhizopus oligospora.
orus), Rhizopus oryzae,
Discovered that fermentation products obtained by the action of one or more of Rhizopus arrhizus or Rhizopus stolonifer have an extremely effective action on spots, freckles, etc. Then, based on this finding, the present invention was completed.

In addition, as a fermentation product obtained by allowing a microorganism belonging to the genus Rhizopus to act on soybean, there is a traditional fermented food in Indonesia, Tempeh, which is an excellent fermented food similar to natto existing in Japan. It is.

However, they are used exclusively for food,
There are no examples used as external preparations such as skin cosmetics.

The purpose of this fermentation product is to provide a new material that is effective and safe for spots, freckles, spots, etc. by incorporating it into skin cosmetics.

(Means for Achieving the Object) In the present invention, soybeans or a medium containing soybeans are added to Rhizopus oligosporus, Rhizopus oryzae, Rhizopus arrhizus or Rhizopus stroniifa (Rhizopus stolonifer) The above object is achieved by providing a skin cosmetic characterized by containing a fermentation product obtained by reacting at least one of Rhizopus stolonifer.

(Constitution of the Invention) The medium used in the present invention is not particularly limited,
Any medium can be used as long as the fungus used in the practice of the present invention satisfactorily propagates, and any medium containing soybean or a component thereof can be used, and a processed product of soybean, a cooked product, or a composition separated from soybean is preferable. Used for

Normally boiled soybeans are used as is, but if necessary,
Soybeans or defatted soybeans, soybean components such as soy milk, yeast extract, casein, whey protein, skim milk, protein degradation products, various amino acids, nitrogen sources such as inorganic nitrogen salts,
Glucose, fructose, carbon sources such as various starches,
Inorganic salts and vitamins can be added for use.

Bacteria suitably used in the fermentation of the present invention include Rhizopus oligosporus, Rhizopus oryzae, and Rhizopus alizas (R)
hizopus arrhizus or Rhizopus stolonifer can be exemplified, and one or more of these fungi can be used in combination.

These bacterial species are all known microorganisms, but the strain is not particularly limited, and those isolated from the natural world and artificial or natural mutants thereof can be used.

Furthermore, since the fungus used in the present invention is parasitic on the leaves of broad-leaved trees such as bananas, teak, and laurel, similarly to the production of tempeh, the leaves of these plants are dried in the shade to form a powder, It can also be used as an inoculum.

The method of fermenting the bacterium used in the present invention by allowing it to act on a medium containing soybeans varies depending on the type of bacterium used and the type of medium, but in practice, the following methods are convenient and advantageous. Such a method can be mentioned.

Soybeans are carefully selected, washed and then immersed in water to allow sufficient water absorption.

At this time, since germs may propagate, it is recommended that the
Lactic acid bacteria that add about 1% lactic acid or propagate well in soybean soaks, such as Lactobacillus plantarum
Adding illus plantarum) to lower the pH can prevent bacterial growth.

Soybeans that have absorbed water are completely removed from the seed coat and boiled for 1 hour at 100 ° C.

The boiled soybeans are thoroughly drained, and then inoculated with the spores of the bacterium used in the present invention when the temperature drops to 40 ° C. or less.

A fermentation product can be obtained by culturing in a fermentation room at a temperature of 30 to 40 ° C and a humidity of 70% or more for 24 to 72 hours.

In actually using these fermentation products,
It is possible to use the fermented product as it is, but since it contains a large amount of insoluble solids such as soybeans, it is possible to use a fermented solution or a solvent extract obtained by squeezing the fermented product or a concentrate thereof more effectively. Can be done.

In the case of a squeezed fermentation solution, a large amount of the active ingredient may remain in the residue, so that it is more preferably used by extracting with a suitable solvent.

The solvent to be used is not particularly limited as long as it is a solvent capable of effectively extracting a component having a preventive or therapeutic effect on stains, freckles, and the like from fermented products. More preferably, water or ethanol or a mixture thereof can be used.

The fermentation solution or fermentation extract thus obtained can be used alone as it is as a skin cosmetic, but it is used as a raw material for skin cosmetic by blending it with ordinary lotions, emulsions, creams, etc. can do.

In this case, it is more preferable to use a concentrate or a dried product.

The fermentation product thus obtained acts very effectively for the prevention or treatment of spots, freckles, and so on,
It turned out to be revived to lively skin.

The active ingredient in the fermentation product and its mechanism of action are not yet clear, and are under intensive study at present. The following facts are considered to be one of the reasons.

That is, it is known that 4 ', 6,7-trihydroxyisoflavone, which is not detected in soybean, is detected in the fermentation product used in the skin cosmetic of the present invention.

This substance has the molecular formula C ₁₅ H ₁₀ O ₅ , melting point 319 (decomp), U
Vλmax: a known compound with 260,327 (mμ), which has a very strong inhibitory effect on melanin production in a melanin pigmentation inhibition test, and is therefore suspected to be one of the important factors for its effectiveness against spots, freckles, etc. Absent.

However, it is difficult to attribute the excellent effectiveness of the fermentation product obtained by allowing a fungus belonging to the genus Rhizopus to act on soybeans solely to the presence of this substance.

The fermentation product contains a large number of other unknown compounds, which are considered to exert an effect by acting additively or synergistically.

In addition, the fermentation product contains various amino acids, peptides, various sugars, various organic acids such as citric acid and pyroglutamic acid, various vitamins such as vitamin B and nicotinic acid, and various trace inorganic salts. And imparts more desirable properties as skin cosmetics, such as prevention of spoilage.

Therefore, it is not always necessary to identify and isolate the active ingredient before use in skin cosmetics.

Incidentally, 4 ', 6,7-trihydroxyisoflavone may be obtained by other means and contained in skin cosmetics.

(Effect of the Invention) The present invention contains a fermentation product obtained by reacting soybean or a medium containing soybean with one or more of Rhizopus oligosporus, Rhizopus oryzae, Rhizopus alizas or Rhizopus stronifera. Since it is a skin cosmetic characterized by the fact that it works, it works extremely effectively for the prevention or treatment of spots, freckles, blemishes, etc., and can be revived to lively skin.

Furthermore, the fermentation product contains various amino acids, peptides, various sugars, organic acids such as citric acid, vitamins, and various trace inorganic salts, and has an excellent moisturizing effect. It also has excellent performance as a compounding material for cosmetics.

Next, the effects of the present invention will be further clarified by describing examples and experimental examples of the present invention.

(Examples and Experimental Examples) Example 1 (Preparation of Extract of Fermented Product Obtained by Applying Rhizopus Oligosporous to Soybean) 100 g of a carefully selected soybean was taken, washed well with water, and then washed with water.
% Lactic acid aqueous solution to absorb water sufficiently.

The soybean that has absorbed water is completely removed from the seed coat and boiled at 100 ° C. for 1 hour.

Boiled soybeans are quickly spread in a container and cooled while removing moisture as much as possible.

When the temperature drops to 90 ° C., add about 1% starch and mix well to partially gelatinize.

When the temperature further drops to 40 ° C, 37 g of defatted soybeans
After inoculating spores of Rhizopus oligosporus cultured at 7 ° C. for 7 days, mix well, deposit so that the temperature does not decrease, and perform fermentation for about 5 hours.

Next, the mixture is put into a plastic tray to a thickness of about 2 cm, put into a fermentation room, and cultured at a temperature of 37 ° C and a humidity of 75% for 48 hours.

 About 180 g of a massive fermentation product was obtained.

The fermented product was quickly mixed well with 600 cc of a mixed solution of water and ethanol (9 parts of water, 1 part of ethanol) with a mixer, followed by suction filtration, and the residue was thoroughly washed with the mixed solution to obtain 1 fermented product extract.

Further, this filtrate was sterilized and filtered with a membrane filter.

Example 2 (Preparation of extract of fermented product obtained by reacting soybean with Rhizopus oryzae) Using Rhizopus oryzae as a seed fungus, cultivation and extraction were performed in the same manner as in Example 1, and fermentation extract was used. I got

However, the fermentation conditions were a fermentation room temperature of 30 ° C. and a fermentation time of 72 hours.

Example 3 (Preparation of an extract of a fermented product obtained by reacting Rhizopus alizas on soybeans) Using Rhizopus alizas as the inoculum, cultivation and extraction were performed in the same manner as in Example 2, and a fermentation extract extract I got

Example 4 (Preparation of Extract of Fermented Product Obtained by Acting Rhizopus stroniipher on Soybean)
Culture and extraction were performed in the same manner as in Example 2 to obtain a fermented product extract.

Example 5 (Preparation of Fermentation Solution Obtained by Applying Rhizopus Oligosporus to Soybean-Containing Medium) To soymilk obtained from 100 g of carefully selected soybean, 5 g of skim milk,
1 g of glucose and 0.5 g of yeast extract are added, and water is further added to prepare a liquid medium having a total amount of 1.

This was put in a two-volume jar, sterilized at 120 ° C. for 15 minutes, and inoculated with Rhizopus oligosporus inoculum in this medium, and incubated at 30 ° C. for 96 hours.

After filtration of this culture by suction, the filtrate was further sterilized and filtered through a membrane filter to obtain about 950 cc of a fermentation solution.

<Experimental Example 1> (Test for inhibiting melanin pigment formation of fermented product extract or fermented solution obtained in Examples 1, 2, 3, 4, and 5, and 4 ', 6,7-trihydroxyisoflavone) L-tyrosine ( 1.0 mg / cc) 0.5 cc, M / 15 phosphate buffer (pH 6.8) 2.0 cc, copper sulfate (1% solution) 0.05 cc, tyrosinase (1 mg / cc) 1.0 cc. Add 2.0 cc of the fermented product extract or fermented solution obtained in 3,4,5 or 2.0 cc of 4 ', 6,7-trihydroxyisoflavone solution (0.0001%), mix and incubate at 37 ° C for 30 minutes, The absorbance was measured at a wavelength of 670 nm using a densitometer.

Separately, 2.0 cc of purified water was used in place of the sample as a blank, and the absorbance was determined. By comparison with this, the inhibition rate of melanin pigment formation of each sample was determined.

As a result, as shown in Table 1, they exhibited a strong melanin pigment formation inhibitory action.

<Experimental Example 2> (Effects of skin cosmetics containing fermented product extract obtained in Example 1) Four types of lotions shown in Table 2 below were stained,
The effect on Sobacas and Hadare was tested as follows.

(1) Subjects Stain: 30 women with stains (aged 37 to 56) Sobacas: 25 women with Sobacas (aged 18 to 42)
Aged) Hadaare: 30 girls who are subjectively and subjectively symptomatic
Name (22 years old to 51 years old) (2) Test method For each symptom, subjects were divided into 5 groups, and 4 groups were washed twice daily in the morning and evening with hot water after washing their face with soap, and 25 days and 56 days later. The improvement effect was evaluated.

 The other group served as a control.

(3) Evaluation Stain 1: The color is quite dark and the boundaries are clear.

2: The color is not very dark, but the boundaries are quite clear.

3: The color is extremely light and the boundaries are not clear.

4: No symptom is observed.

Buckwheat 1: Blackish spots spread on the cheeks, sometimes even on the forehead.

2: Brown to grayish spots spread on cheeks.

3: Light brownish spots are observed on the cheeks, but not so noticeably.

4: No symptom of freckles is observed.

Scratch 1: Skin is dry and keratin is easy to peel off, and if you scratch it with your toes, the keratin easily peels off. We always wipe white powder.

2: The skin is rusty, exfoliated in places, and slightly white powdered.

3: The skin is dry, but the keratin is not peeled off or wiped with white powder.

4: The skin is moist and smooth.

 The following evaluation was made according to the classification of the symptoms.

Effective: Symptoms improved from 1 to 3,4 and from 2 to 4.

Somewhat effective: Symptoms 1 to 2, 2 to 3 and 3 to 4
What has been improved.

Ineffective: Little or no change in symptoms.

 The evaluation results are shown in Tables 3 and 4.

As is clear from the results, each of the lotions containing the fermented product extract obtained in Example 1 has excellent effects. Above all, the effect on slime is remarkable on the 28th day, and is immediate and persistent. The effect on spots is prominent on day 56 and is somewhat delayed. The effect on buckwheat is slight on day 28 and moderate on day 56.

In contrast, in the control group, no effect was observed at all on spots and freckles, and almost no effect was observed on spoilers.

<Experimental Example 3> (Preparation of cream containing fermented product extract obtained in Example 1) Treatment% by weight Stearic acid 8.0 Cetanol 3.0 Beeswax 3.0 Squalane 6.0 Octyldodecanol 6.0 Polyoxyethylene cetyl ether (25E.O) 2.0) Lipophilic glyceryl monostearate 1.0 Preservative 0.2 Perfume 0.5 Propylene glycol 3.0 Glycerin 3.0 Borax 0.4 Fermented product extract obtained in Example 1 20.0 Purified water Remainder
The method was used for 30 persons (37 to 54 years old) (21 out of 30 persons were found to be harmful at the same time) and evaluated by the method performed in Experimental Example 2. The following evaluation results were obtained.

Effect on stains Day 28 Day 56 Effective 7/30 19/30 Slightly effective 18/30 9/30 Ineffective 5/30 2/30 Effect on barefoot day 28 Day 56 Effective 16/21 19/21 Slightly effective 4/21 2/21 Ineffective 1/21 0/21 Excellent effects were observed as described above.

Claims (4)

(57) [Claims] 1. A method for preparing soybeans or a medium containing soybeans, wherein Rhizopus oligosporus, Rhizopus oryzae, Rhizopus oryzae,
A skin cosmetic comprising a fermentation product obtained by allowing at least one of Rhizopus arrhizus and Rhizopus stolonifer to act. 2. The skin cosmetic according to claim 1, wherein said soybean is a processed product of soybean, a cooked product or a composition separated from soybean. 3. The skin cosmetic according to claim 1, wherein the fermentation product is a fermentation product, a fermentation solution or an extract thereof. 4. The fermentation product according to claim 1, wherein the fermentation product is prepared by adjusting or processing a fermentation product to contain 4 ', 6,7-trihydroxyisoflavone. Skin cosmetics.