JP2607115B2 - Skin cosmetics - Google Patents

Description

The present invention relates to new skin cosmetics (including quasi-drug cosmeceuticals and medicinal dermatological preparations) which are effective in normalizing human skin. The same), the purpose is to provide a skin cosmetic which is effective for prevention or treatment of spots, freckles, and the like.

(Prior art and its problems) Conventionally, as a skin cosmetic, it has been proposed to mix various drugs for the prevention or treatment of spots, freckles, etc., but its efficacy, safety or stability From the point of view, few are satisfactory.

In recent years, vitamin C or its derivatives, aloe extract, placenta extract, glutathione, etc. have been used for this purpose, but are not yet satisfactory.

That is, vitamin Cs, placenta extract, and glutathione have a problem in stability after preparation, and aloe extract has a defect of browning over time.

(Progress of the Invention) As a result of intensive studies to develop a truly effective skin cosmetic, the present inventors have found that a fermentation product obtained by allowing peanut or soybean to act on Neurospora sitophila is: It has been found that it has a very effective effect on spots, freckles, and so on.

Peanuts or soybeans, Neurospora sitophila (Neur
As a fermentation product obtained by the action of ospora sitophila, there is Ontjom, a traditional fermented food in Indonesia, which is an excellent fermented food similar to natto existing in Japan.

Peanut or peanut meal is mainly used, but soybean is sometimes used instead of peanut as a raw material, and is not clearly distinguished.

These are exclusively used for food, and no examples have been used as external preparations such as skin cosmetics.

The present inventors have found that this fermentation product is effective for spots, freckles, and so on, and have completed the present invention based on this fact.

(Means for Solving the Problems) In the present invention, a medium containing peanut and / or soybean, or casein peptone, skim milk, malt extract, yeast extract, potato extract, corn extract, various protein decomposed products, amino acids, etc. Organic nitrogen sources, ammonium salts, nitrogen sources selected from at least one inorganic nitrogen sources such as nitrates, and glucose, galactose, raffinose, sucrose, lactose, dextrin, a medium containing at least one carbon source selected from glycerin Neurospora sitophi
2. The skin cosmetic composition according to claim 1, wherein the skin cosmetic composition contains a fermentation product obtained by acting la), and the fermentation product is a fermentation product, a fermentation solution or an extract thereof. 3. The skin cosmetic according to claim 1, wherein the fermentation product is Ontjom, and a composition in which peanuts and / or soybeans are processed, cooked or separated therefrom. The above problem is solved by providing the skin cosmetic according to any one of claims 1 to 3, characterized in that:

(Constitution of the Invention) Neurospora sitophila
The medium used in the reaction is not particularly limited, but the bacterium used in the practice of the present invention can be satisfactorily propagated, and the fermentation product has a composition that effectively acts on spots, freckles, and spoilers. Any medium may be used as long as it contains.

Any nitrogen source can be used as long as Neurospora sitophila can be used. For example, casein peptone, skim milk, malt extract, yeast extract, potato extract, corn extract,
Various types of protein degradation products, organic nitrogen sources such as amino acids, and inorganic nitrogen sources such as ammonium salts and nitrates can be used alone or in combination.

Carbon sources include Neurospora sitophila
Any substance can be used as long as pora sitophila can be used, and glucose, galactose, raffinose, sucrose, lactose, dextrin, glycene and the like can be used.

In addition, if necessary depending on the culture medium, phosphates, hydrochlorides, magnesium salts, potassium salts, and other inorganic salts, vitamins, amino acids, lipids, or a proliferative factor rich in these should be added. Can be.

However, as is clear from the course of the invention, more preferably, heat-treated peanut meal or soybean is used.

In other words, a method for producing ontjom is sufficient.

Usually, steamed peanuts, peanut meal or soybeans are used as they are, but if necessary, the above-mentioned nitrogen source, carbon source, growth factor, etc. can be added thereto.

Neurospora sitophila
The fermentation method varies depending on the origin of the bacterium used and the type of culture medium, but practically convenient and advantageous methods include the following methods.

The peanuts are carefully selected, washed, and then immersed in water overnight to allow sufficient water absorption.

The peanuts that have absorbed water are removed from the seed coat and steamed.

Steamed peanuts, after draining well, corn,
A starch such as rice or cassava is added to form a link and molded into a kamaboko mold.

This is inoculated with spores and cultured in a fermentation room at a temperature of 27 to 30 ° C. for 24 to 48 hours to obtain a fermented product.

As the Neurospora sitophila used in the fermentation of the present invention, any strain may be used, and those isolated from the natural world and artificial or natural mutants thereof can be used.

In actually using these fermentation products,
It is possible to use the fermented product as it is, but since it contains a large amount of insoluble solids such as peanuts, the fermented product obtained by squeezing or filtering the fermented product, or a solvent extract or a concentrate thereof is more effective. Can be used for

 More preferably, it is used after extraction with a suitable solvent.

The solvent to be used is not particularly limited as long as it is a solvent that can effectively extract a component having a preventive or therapeutic effect on stains, freckles, and the like from fermented products.

More preferably, water or ethanol or a mixture thereof can be used.

The resulting Neurospora Citophila (Neuros
The fermentation solution or fermentation extract obtained by the action of pora sitophila can be used alone as it is as a skin cosmetic, but it can be used as a raw material for the skin cosmetic, such as a normal lotion, emulsion, cream, etc. Can be used in combination.

In this case, it is more preferable to use a concentrate or a dried product.

The fermentation product thus obtained acts very effectively for the prevention or treatment of spots, freckles, and so on,
It turned out to be revived to lively skin.

The mechanism of action of the fermentation product is not yet clear.

In the fermentation products, various amino acids, peptides, various sugars, various organic acids such as citric acid, vitamins
It contains various vitamins such as B ₆ , vitamin B ₁₂ and nicotinic acid, and various trace inorganic salts and the like, and imparts properties favorable as skin cosmetics.

In particular, Neurospora sitopira
As a feature of hila), it produces a large amount of a carotenoid pigment having a vitamin A effect, and is extremely effective for prevention of spoilage.

The fermentation product contains a large number of other unknown compounds, which are considered to exert an effect by acting additively or synergistically.

Therefore, it is not always necessary to identify and isolate the active ingredient before use in skin cosmetics.

Also, as a feature that is particularly characterized, the fermented product emits a sweet fruit-like ester fragrance, imparts superior performance to cosmetics whose life is fragrant, and is regarded as a problem in terms of safety. There is no need to add synthetic flavors.

(Effects of the Invention) The present invention relates to a medium containing peanut and / or soybean, or casein peptone, skim milk, malt extract, yeast extract, potato extract, corn extract, various protein decomposed products, organic nitrogen sources such as amino acids, and ammonium. Salts, nitrogen sources selected at least one from inorganic nitrogen sources such as nitrates,
And a skin cosmetic containing a fermentation product obtained by allowing Neurospora sitophila to act on a medium containing at least one carbon source selected from glucose, galactose, raffinose, sucrose, lactose, dextrin, and glycerin. Therefore, it can act very effectively for the prevention or treatment of spots, freckles, and so on.

Next, the effects of the present invention will be further clarified by describing examples and experimental examples of the present invention.

(Examples and Experimental Examples) Example 1 (Preparation of Extract of Fermented Product Obtained by Applying Neurospora sitophila to Peanuts) Take 100 g of carefully selected peanuts, wash well with water, and immerse in water overnight And let it absorb enough water.

The water-absorbed peanuts are removed from the seed coat and steamed at 100 ° C. for 1 hour.

The steamed peanuts are spread quickly in a container and cooled while removing moisture as much as possible.

When the temperature drops to 90 ° C, about 10% of raw starch is added, mixed well, and a portion is gelatinized to form a binder.

When the temperature further dropped to 30 ° C., the corn was molded into a kamaboko mold, placed on a plastic tray, and preheat-sterilized corn was inoculated with Neurospora sitophila by slope culture, and then inoculated at 27 ° C. for 3 days. After cultivation, inoculate the cells that have been dried at low temperature.

The mixture was placed in a fermentation room and cultured at a temperature of 27 ° C. for 48 hours to obtain about 120 g of a fermented product.

The fermented product was quickly mixed well with 600 cc of a mixed solution of water and ethanol (9 parts of water, 1 part of ethanol) with a mixer, followed by suction filtration, and the residue was thoroughly washed with the mixed solution to obtain 1 fermented product extract.

Further, this filtrate was sterilized and filtered with a membrane filter.

Example 2 (Neurospora Citophila (Neur
Preparation of extract of fermented product obtained by the action of ospora sitophila) 60 g of hot water is added to 60 g of defatted peanut lees and steamed at 100 ° C. for 1 hour.

This was treated in the same manner as in Example 1 to obtain a fermented product extract.

Example 3 (Neurospora sotophyla (Neurospo
Preparation of Extract of Fermented Product Obtained by Acting with Ra sitophila) 100 g of selected soybeans are washed, washed with water, immersed overnight in a 0.1% lactic acid aqueous solution, and sufficiently absorbed.

The soybean that has absorbed water is removed from the seed coat and steamed at 100 ° C. for 1 hour.

This was treated in the same manner as in Example 1 to obtain a fermented product extract.

Example 4 (Neurospora sitophila
Preparation of Extract of Fermented Product Obtained by Acting S. ophila) Water is added to 30 g of corn extract, 5 g of skim milk, 1 g of glucose, and 0.5 g of yeast extract to prepare a liquid medium having a total volume of 1.

This was put in a two-volume jar, sterilized at 120 ° C. for 15 minutes, and this medium was inoculated with a seed of Neurospora sitophila and cultured at 30 ° C. for 96 hours with shaking.

After filtration of this culture by suction, the filtrate was further sterilized and filtered through a membrane filter to obtain about 950 cc of a fermentation solution.

Experimental Example 1 (Test for inhibition of melamine pigment formation in fermented product extract or fermented solution obtained in Examples 1, 2, 3, and 4) L-tyrosine (1.0 mg / cc) 0.55 cc, M / 15 phosphate buffer ( pH 6.8) 2.0 cc, copper sulphate (1% solution) 0.05 cc, tyrosinase (1 mg / cc) 1.0 cc, fermented product extract or fermented solution 2.0 cc obtained in Examples 1, 2, 3 and 4 as specimens And mix at 37 ° C for 30 minutes.
After incubating for a minute, the absorbance was measured at a wavelength of 670 nm using a spectrophotometer.

Separately, as a blank, 2.0 cc of purified water was used instead of the sample, and the absorbance was determined. By comparing the absorbance, the inhibition rate of melanin pigment formation of each sample was determined.

As a result, as shown in Table 1, they exhibited a strong melanin pigment formation inhibitory action.

Experimental Example 2 (Effect of skin cosmetic containing fermented product extract obtained in Example 1) Four types of lotions shown in Table 2 below were stained,
The effect on Sobacas and Hadare was tested as follows.

(1) Subjects Stain: 25 women with stains (24-48 years old) Sobacas: 25 women with Sobacas (21-52 years old)
Aged) Hadaare: Girls with subjectively objective Hadare symptoms
Name (27-65 years old) (2) Test method For each symptom, subjects were divided into 5 groups, and 4 groups were washed twice a day with soap and hot water twice a day after washing their face with warm water, and after 28 and 56 days The improvement effect was evaluated.

 The other group served as a control.

(3) Evaluation spot 1: The color is quite dark and the boundaries are clear.

2: The color is not very dark, but the boundaries are quite clear.

3: The color is extremely light and the boundaries are not clear.

4: No symptom is observed.

Buckwheat 1: Blackish spots spread on the cheeks, sometimes even on the forehead.

2: Brown to grayish spots spread on cheeks.

3: Light brownish spots are observed on the cheeks, but not so noticeably.

4: No symptom of freckles is observed.

Scratch 1: Skin is dry and keratin is easy to peel off, and if you scratch it with your toes, the keratin easily peels off. We always wipe white powder.

2: The skin is rusty, exfoliated in places, and slightly white powdered.

3: Although the skin is dry, there is no peeling of keratin or wiping of white powder.

4: The skin is moist and smooth.

 The following evaluation was made according to the classification of the symptoms.

Effective: Symptoms improved from 1 to 3,4 and from 2 to 4.

Somewhat effective: Symptoms 1 to 2, 2 to 3 and 3 to 4
What has been improved.

Ineffective: Little or no change in symptoms.

 The evaluation results are shown in Tables 3 and 4.

As is clear from the results, each of the lotions containing the fermented product extract obtained in Example 1 has excellent effects.

Above all, the effect on rustle is quick and continuous.

The effect on spots and freckles is prominent on day 56 and is somewhat delayed.

In contrast, in the control group, no effect was observed at all on spots and freckles, and almost no effect was observed on spoilers.

Experimental Example 5 (Preparation of cream containing fermented product extract obtained in Example 1) Treatment% by weight Stearic acid 2.0 Stearyl alcohol 7.0 Reduced lanolin 2.0 Squalane 6.0 Octyldodecanol 5.0 Polyoxyethylene cetyl ether (25E.O) 3.0 Glycerin monostearate 2.0 Lipophilic type 2.0 Preservative 0.2 Propylene glycol 3.0 Glycerin 3.0 Borax 0.4 Fermented product extract obtained in Example 1 20.0 Purified water Remainder The effect of cream prepared by the above formulation on stains and the like is shown in Experimental example 2. The test was conducted in the same manner as in the above.

 However, the subjects are as follows.

Spots: 10 girls with spots (aged 37-58) Sobacas: 10 girls with spots (19-33 years old)
Years old) Hadare: Boys with Hadare's symptoms subjectively and objectively 10
Names (23-44 years old), 10 girls (23-44 years old) Evaluation results are shown in Tables 5 and 6.

Claims (4)

(57) [Claims] 1. A medium containing peanut and / or soybean, or casein peptone, skim milk, malt extract, yeast extract, potato extract, corn extract, various protein decomposed products, organic nitrogen sources such as amino acids, ammonium salts, nitrates A nitrogen source selected at least one from inorganic nitrogen sources such as
And a fermentation product obtained by allowing Neurospora sitophila to act on a medium containing a carbon source selected from at least one of glucose, galactose, raffinose, sucrose, lactose, dextrin, and glycerin. Skin cosmetics. 2. The skin cosmetic according to claim 1, wherein the fermentation product is a fermentation product, a fermentation solution or an extract thereof. 3. The skin cosmetic according to claim 1, wherein the fermentation product is Ontjom. 4. The skin cosmetic according to claim 1, wherein the peanut and / or soybean is a processed product thereof, a cooked product thereof, or a composition separated therefrom.