JP2870871B2 - A method for treating crustacean shells using enzymes - Google Patents
A method for treating crustacean shells using enzymesInfo
- Publication number
- JP2870871B2 JP2870871B2 JP1275330A JP27533089A JP2870871B2 JP 2870871 B2 JP2870871 B2 JP 2870871B2 JP 1275330 A JP1275330 A JP 1275330A JP 27533089 A JP27533089 A JP 27533089A JP 2870871 B2 JP2870871 B2 JP 2870871B2
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- Prior art keywords
- chitin
- calcium carbonate
- treatment
- protein
- crustacean
- Prior art date
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Description
【発明の詳細な説明】 産業上の利用分野 本発明は酵素を用いる甲殻類の甲殻（クチクラ）の処
理方法に関するもので、特にエビ、カニ等の甲殻類のク
チクラ（甲殻外骨格）に放線菌由来のプロテアーゼであ
るアクチナーゼを作用させることによって蛋白質及び炭
酸カルシウムを分離したものを、さらに酸処理してキチ
ンを単離精製し、甲殻の他の主構成成分たる蛋白質をア
ミノ酸水溶液、炭酸カルシウムは天然のままの炭酸カル
シウムの沈殿として取得することでクチクラ中のすべて
の有効成分を利用するためのクチクラの処理方法に関す
るものである。Description: FIELD OF THE INVENTION The present invention relates to a method for treating crustacean crustacea (cuticle) using an enzyme, and particularly to actinomycetes on crustacean cuticle (crustacean exoskeleton) such as shrimp and crab. The protein and calcium carbonate separated by the action of actinase, a protease derived from the protein, are further treated with acid to isolate and purify chitin. The present invention relates to a method for treating cuticles in order to utilize all active ingredients in cuticles by obtaining as-is calcium carbonate precipitate.
従来の技術 地球上にあって最も豊富かつ利用されているバイオマ
ス資源であるセルロースの自然界における生産量は、年
間1000億トンともいわれるが、キチンの年間生産量もま
た100億、あるいはセルロースに匹敵する1000億トンと
もいわれている。キチンは今までのところ、その脱アセ
チル生成物であるキトサンに変換され凝集剤として水処
理に利用されているほか、近年になってキチン、キトサ
ンの持つ化学的あるいは生物学的な特性機能が解明さ
れ、それに基づき食品、化粧品、医薬、農薬等の分野に
おいて活発な利用研究が行われ、特徴ある新製品が生み
出されている。キチンはＮ−アセチル−Ｄ−グリコサミ
ン残基が多数β−（1,4）結合した多糖で、甲殻類並び
に昆虫類の外皮骨格組成、菌類の細胞壁の構成成分とし
てよく知られている。エビ、カニ等の甲殻類のクチクラ
は炭酸カルシウム、蛋白質、キチンを主成分として構成
され、一般にこの甲殻（クチクラ）の化学的処理により
キチンの工業的製造が行われており、また、実験的には
微生物、酵素を用いた生物化学的処理によるキチンの製
造も検討されている。Conventional technologyThe natural production of cellulose, the most abundant and most used biomass resource on earth, is said to be 100 billion tons per year, but the annual production of chitin is also 10 billion, or comparable to cellulose It is said to be 100 billion tons. So far, chitin has been converted to its deacetylated product, chitosan, and used as a flocculant in water treatment.In recent years, the chemical or biological properties of chitin and chitosan have been elucidated. Based on this, active utilization research has been carried out in the fields of food, cosmetics, medicine, agrochemicals and the like, and characteristic new products have been produced. Chitin is a polysaccharide having a large number of β- (1,4) -linked N-acetyl-D-glycosamine residues, and is well known as a constituent of the shell skeleton composition of shellfish and insects and a cell wall of fungi. Cuticles of crustaceans such as shrimp and crab are composed mainly of calcium carbonate, protein and chitin. Generally, chitin is produced industrially by chemical treatment of this crustacean (cuticle). Is also studying the production of chitin by biochemical treatment using microorganisms and enzymes.
キチンの製造工程自体はそう複雑なものではないが、
化学処理法は製造工程で、酸処理とアルカリ処理を必要
とするため、洗浄等に大量の水を必要とし廃水の処理に
多額の費用がかかるほか、また、酸処理工程の温度が分
子量に影響する等、アルカリや酸の濃度、反応時間、反
応温度等の反応条件の違いによって、得られる製品の純
度や品質が異なってくるばかりでなく、更に熱アルカリ
や酸によるグリコシド結合の加水分解による分子量低下
を避け得ない欠点がある。The production process of chitin itself is not so complicated,
The chemical treatment method requires a large amount of water for washing and the like because it requires an acid treatment and an alkali treatment in the manufacturing process, and wastewater treatment costs a great deal of money.In addition, the temperature of the acid treatment process affects the molecular weight. Due to differences in reaction conditions such as alkali and acid concentrations, reaction time, reaction temperature, etc., not only does the purity and quality of the resulting product differ, but also the molecular weight due to hydrolysis of glycosidic bonds by hot alkalis and acids. There are drawbacks that cannot be avoided.
これらの化学処理法の欠点を解決すべく、微生物や酵
素を用いた生物化学的処理方法が研究されるようにな
り、キチン製造における除蛋白工程にプロテアーゼを用
いることが試みられ、プロナーゼ、パパイン、ペプシン
など種々のプロテアーゼを用いた実験が行われているほ
か、最近ではプロテアーゼ生産菌を用いることも試みら
れている。これら生物化学的処理方法は従来のアルカリ
処理法と比較して脱アセチル、解重合などの副反応を起
こさないため、変性の少ない比較的高分子量のキチンが
得られるほか、処理温度が低いなどの利点があるもの
の、一貫した生物化学処理による蛋白質の完全除去はい
まだ達成されてなく、あくまでも酸処理による除カルシ
ウム工程を前提にしたものであって、しかもキチン以外
の甲殻（クチクラ）の主構成成分たる蛋白質や炭酸カル
シウムの利用に関しては何等の感心が持たれていない実
態にある。In order to solve the drawbacks of these chemical treatment methods, biochemical treatment methods using microorganisms and enzymes have been studied, and attempts have been made to use proteases in the deproteinization step in the production of chitin, pronase, papain, In addition to experiments using various proteases such as pepsin, recently, attempts have been made to use protease-producing bacteria. Since these biochemical treatment methods do not cause side reactions such as deacetylation and depolymerization compared to the conventional alkali treatment methods, relatively high-density chitin with little modification can be obtained, and the treatment temperature is low. Despite the advantages, complete removal of protein by consistent biochemical treatment has not yet been achieved, and it is based on the pre-calcification process by acid treatment, and is the main constituent of cuticle other than chitin There is no interest in the use of protein and calcium carbonate.
発明が解決しようとする問題点 従って本発明は生物化学的処理による高分子量キチン
製造に際し、化学処理工程を出来るだけ少なくするため
のものであり、特に化学処理工程における酸処理工程を
不要にする生物化学的処理によるキチンの製造法を提供
するためのものであって、その際の蛋白質の完全除去と
副生成分の有効利用を計るためのものである。Problems to be Solved by the Invention Accordingly, the present invention is intended to minimize the number of chemical treatment steps in the production of high molecular weight chitin by biochemical treatment. The purpose of the present invention is to provide a method for producing chitin by a chemical treatment, in which the complete removal of proteins and the effective use of by-products are measured.
問題点を解決するための手段 すなわち、本発明はエビ、カニ等の甲殻類の甲殻（ク
チクラ）に放線菌由来のプロテアーゼであるアクチナー
ゼを作用させることによって蛋白質及びカルシウムを除
去分離した後、キチン中の残存カルシウムを酸処理によ
り除去することを特徴とする甲殻類の甲殻（クチクラ）
の処理方法に関するもので、本発明の方法では先ず、甲
殻を数平方センチメーターの大きさのチップに切断する
が、細かすぎると以下に述べる炭酸カルシウムとの分離
が難しくなる。勿論、酵素の作用に関しては如何に細か
くしても不都合ということはない。次に、pH5〜７に調
整した水に、切断した甲殻とアクチナーゼを加える。こ
の場合最適pHは６である。アクチナーゼのカゼインに対
する最適pHは科研製薬（株）の試料では7.0〜9.0である
が、本発明の最適pHが６であるのは甲殻に含まれる炭酸
カルシウムによる影響が考えられる。アクチナーゼはカ
ルシウムイオンにより安定化することが知られ、特に耐
熱性が増加することが示されている。これらのことから
炭酸カルシウムを含む甲殻試料の蛋白質分解にはアクチ
ナーゼが極めて適しているものと考えられる。次に試料
を加えた液を30〜60℃で撹はんするが最適温度は50℃で
あり、それから離れるにつれ蛋白分解速度は小さくな
る。反応時間は夫々の条件において適宜定めるが蛋白質
の分解につれ、炭酸カルシウムが沈殿となってかなり除
去される。これはキチンと共に甲殻マトリクスを形成し
ている蛋白質がいわば接着剤的役割を果たして炭酸カル
シウムをそのマトリクスの構成成分としていたのが、蛋
白質という接着剤の分解に伴ない炭酸カルシウムが離脱
するものと思われる。この炭酸カルシウムは非常に微細
な粒子であるがろ過により蛋白質が分解して生成したア
ミノ酸水溶液からキチンと共に分離される。この炭酸カ
ルシウムとキチンは粒度の違いによりろ過等の通常の物
理的手段で容易に分離される。ここで分離されたキチン
にはまだ一部の炭酸カルシウムンが付着しているが、塩
酸またはEDTAで処理することで炭酸カルシウムを完全に
除去したキチンを得ることができる。Means for Solving the Problems That is, the present invention removes and separates protein and calcium by allowing actinase, a protease derived from actinomycetes, to act on the crustacean (cuticle) of crustaceans such as shrimp and crab. Crustacean crustaceans characterized by the removal of residual calcium by acid treatment
In the method of the present invention, the shell is first cut into chips having a size of several square centimeters, but if it is too fine, it will be difficult to separate it from calcium carbonate described below. Of course, no matter how detailed the action of the enzyme is, there is no disadvantage. Next, the cut crust and actinase are added to water adjusted to pH 5-7. In this case, the optimum pH is 6. The optimum pH of actinase for casein is 7.0-9.0 in the sample of Kaken Pharmaceutical Co., Ltd., but the optimum pH of the present invention is 6 due to the effect of calcium carbonate contained in the shell. Actinase is known to be stabilized by calcium ions, and has been shown to increase heat resistance in particular. From these facts, it is considered that actinase is extremely suitable for proteolysis of crustacean samples containing calcium carbonate. Next, the solution containing the sample is stirred at 30 to 60 ° C., but the optimum temperature is 50 ° C., and the further apart, the lower the proteolytic rate. The reaction time is appropriately determined under each condition, but as the protein is decomposed, calcium carbonate precipitates and is considerably removed. This is because the protein that forms the shell matrix together with chitin played an adhesive role, so to speak, and calcium carbonate was a component of the matrix.It seems that calcium carbonate is released with the decomposition of the protein adhesive. It is. This calcium carbonate is very fine particles, but is separated together with chitin from an amino acid aqueous solution generated by decomposing proteins by filtration. The calcium carbonate and chitin are easily separated by ordinary physical means such as filtration due to the difference in particle size. Although some calcium carbonate still adheres to the chitin separated here, chitin from which calcium carbonate has been completely removed can be obtained by treatment with hydrochloric acid or EDTA.
実施例 以下に本発明の実施例を示すが、これは本発明の一例
を示すのみであって、これらの実施例により本発明が何
等限定されるものではないことは当然である。Examples Examples of the present invention will be described below, but these are merely examples of the present invention, and it is a matter of course that the present invention is not limited to these examples.
実施例１ １）試料 エビ：ノルウェー産（肉部分を除いた甲殻全体） 水分 10.1 灰分 20.6 粗脂肪 11.0 粗蛋白質 23.9 炭水化物 34.4 ２）操作 0.1Mクエン酸緩衝液500mlにアクチナーゼＥ（高純度
品）200万チロジン単位と10cm2程度に切断したエビ試料
20gを加え、50℃で60時間反応を行い蛋白質の分解を行
った。キチンと炭酸カルシウムはろ過によりアミノ酸水
溶液から分離した。また、キチンと炭酸カルシウムは粒
度の差によって分けた。ここで得たキチンを2N酸塩中48
時間浸漬し無機塩を除去後、蒸留水、エタノール洗浄、
乾燥して精製キチンを得た。Example 1 1) Sample Shrimp: Norway (whole shell except for meat) Moisture 10.1 Ash 20.6 Crude fat 11.0 Crude protein 23.9 Carbohydrate 34.4 2) Operation Actinase E (high purity product) 200 in 500 ml of 0.1 M citrate buffer Shrimp sample cut to about 10 cm 2 with 10,000 tyrosine units
20 g was added and the reaction was carried out at 50 ° C. for 60 hours to decompose the protein. Chitin and calcium carbonate were separated from the aqueous amino acid solution by filtration. In addition, chitin and calcium carbonate were classified according to the difference in particle size. The chitin obtained here was converted to 48
After immersion for a time to remove inorganic salts, distilled water, ethanol washing,
Drying yielded purified chitin.
３）結果 ａ）アミノ酸：得られたアミノ酸組成を表１、表２（１
が一回目の酵素処理、２が二回目）に示し、表３には資
料の蛋白質を塩酸で加水分解したときのアミノ酸組成を
示した。3) Results a) Amino acids: Tables 1 and 2 (1
Indicates the first enzyme treatment, and 2 indicates the second), and Table 3 shows the amino acid composition when the protein in the sample was hydrolyzed with hydrochloric acid.
ｂ）灰分：収量 2.88g 分析の結果から、カルシウムの割合が多く、灰分のほ
とんどが炭酸カルシウムであることが判明した。b) Ash: Yield 2.88 g The results of the analysis revealed that the proportion of calcium was large and that most of the ash was calcium carbonate.
ｃ）キチン：収量 1.43g 蛋白質含量 0.28％ 除去率 99.2 ％ カルシウム含量 0.03％ 除去率 99.7 ％ 表 １ Amino acid μg/g Asparagine 33.81 Threonion 16.08 Serin 14.12 Glutamine 37.80 Proline 11.40 Glycine 20.14 Alanine 22.05 Cystine 2.04 Valine 17.50 Methionine 8.36 Isoleucine 14.85 Leucine 20.60 Tyrosine 16.09 Phenylalanine 15.02 Lysine 20.70 Histidine 6.63 Tryptophan − Arginine 15.07 表 ２ Amino acid μg/g Asparagine 9.74 Treonion 6.50 Serin 4.79 Glutamine 6.30 Proline 2.92 Glycine 5.72 Alanine 5.56 Cystine 0.58 Valine 4.75 Methionine 0.49 Isoleucine 2.67 Leucine 4.10 Tyrosine 6.38 Phenylalanine 3.06 Lysine 4.48 Histidine 1.95 Tryptophan − Arginine 3.53 表 ３ Amino acid μg/g Asparagine 24.32 Threonion 10.97 Serin 15.94 Glutamine 28.60 Proline 12.18 Glycine 19.41 Alanine 14.47 Cystine − Valine 14.63 Methionine 3.42 Isoleucine 7.52 Leucine 12.06 Tyrosine 103.61 Phenylalanine 26.52 Lysine 9.62 Histidine 6.46 Tryptophan − Arginine 18.32 実施例２ １）試料：北海道産毛がに、煮沸処理甲殻 水分 6.41 ％ 灰分 40.00 粗脂肪 3.76 粗蛋白質 37.13 糖質 12.70 ２）操作 酵素反応終了後の脱灰分操作以外は実施例１と同様の
操作を行った。脱灰分は0.1M EDTA溶液（pH7）中で24時
間１回、48時間１回浸漬撹はんして行った。c) Chitin: Yield 1.43g Protein content 0.28% Removal 99.2% Calcium content 0.03% Removal 99.7% Isoleucine 14.85 Leucine 20.60 Tyrosine 16.09 Phenylalanine 15.02 Lysine 20.70 Histidine 6.63 Tryptophan − Arginine 15.07 Table 2 Amino acid μg / g Asparagine 9.74 Treonion 6.50 Serin 4.79 Glutamine 6.30 Proline 2.92 Glycine 5.72 Alanine 5.56 Cystine 0.58 Valine 4.75 Mesine 4.75 3.06 Lysine 4.48 Histidine 1.95 Tryptophan-Arginine 3.53 Table 3 Amino acid μg / g Asparagine 24.32 Threonion 10.97 Serin 15.94 Glutamine 28.60 Proline 12.18 Glycine 19.41 Alanine 14.47 Cystine-Valine 14.63 Methionine 3.42 Isoleucine 7.52 Leucine 12.62 Tyrosine 6.62 Tyrosine Arginine 18.32 Example 2 1) Sample: Hokkaido hair was boiled, crustacea moisture 6.41% ash 40.00 Crude fat 3.76 Crude protein 37.13 Carbohydrate 12.70 2) Operation The same operation as in Example 1 was performed except for the operation of demineralization after completion of the enzyme reaction. The decalcified portion was immersed and stirred once in a 0.1 M EDTA solution (pH 7) for 24 hours and once for 48 hours.
３）結果 キチン：収量 2.1g 蛋白質含量 0.38％ 除去率 98.8 ％ カルシウム含量 trace 除去率 100.0％ 絶対分子量 13.07×105 なお、絶対分子量はキチンを塩化リチウムを含むジメ
チルアセトアミドに溶解し、静的光散乱を利用した光散
乱光度計（大塚電子（株）製ダイナミック光散乱光度計
DSL−700）を用いて測定した。3) Results Chitin: Yield 2.1g Protein content 0.38% Removal rate 98.8% Calcium content trace Removal rate 100.0% Absolute molecular weight 13.07 × 10 5 The absolute molecular weight was determined by dissolving chitin in dimethylacetamide containing lithium chloride and static light scattering. Light scattering photometer (Dynamic light scattering photometer manufactured by Otsuka Electronics Co., Ltd.)
DSL-700).
比較例 １）試料 実施例２と同じ ２）操作 試料（冷凍乾燥後粉砕したもの）44gに2N塩酸400mlを
加え、５時間室温に放置して灰分を除去した。ろ過によ
り甲殻を回収し、更に2N塩酸100mlにて48時間、室温で
連続撹はんし脱灰を行った。脱灰終了後、水洗し沸騰水
浴中で12時間、1N水酸化ナトリウム100mlにて２回除蛋
白を行った。後、水洗、さらにエタノールにて24時間室
温で撹はんし残存する色素を除去、エタノールで洗浄
後、減圧乾燥してキチンを単離精製した。Comparative Example 1) Sample Same as in Example 2 2) Operation To 44 g of a sample (freeze-dried and pulverized), 400 ml of 2N hydrochloric acid was added and left at room temperature for 5 hours to remove ash. The crust was recovered by filtration, and further demineralized by continuously stirring at room temperature for 48 hours with 100 ml of 2N hydrochloric acid. After the decalcification, the protein was washed and deproteinized twice in 100 ml of 1N sodium hydroxide for 12 hours in a boiling water bath. Thereafter, the residue was washed with water, further stirred with ethanol at room temperature for 24 hours to remove the remaining dye, washed with ethanol, and dried under reduced pressure to isolate and purify chitin.
３）結果 絶対分子量 9.70×105 分析方法は実施例２と同じ 発明の効果 本発明によれば、従来の酵素処理法で達成し得なかっ
たほぼ完全な蛋白質の除去が達成できるばかりでなく得
られるキチンも従来得られなかった高分子量のものとな
り、また、原料の甲殻（クチクラ）を微粉末に粉砕する
必要がなく、このことは酵素処理の過程で沈殿する炭酸
カルシウムとキチンの分離を容易にする点で生産上非常
に重要な利点となるもので、今までの酵素処理では考え
られなかったことである。また、本発明によれば、甲殻
（クチクラ）中の蛋白質はアミノ酸水溶液として回収さ
れ肥料、飼料、食品原料等として、一方炭酸カルシウム
は天然に存在したままの形で回収され、医薬等としてそ
れぞれの有効利用への道を開くものである。さらに、本
発明の効果として、酵素処理によりかなりの炭酸カルシ
ウムが沈殿するため、その後のキチンの酸処理で使用す
る酸の量を少なくできるため経済的であるばかりでな
く、酸による変性をも少なくすることができる。3) Results The absolute molecular weight of 9.70 × 10 5 is the same as in Example 2. Advantageous Effects of the Invention According to the present invention, almost complete removal of proteins that could not be achieved by the conventional enzymatic treatment method can be achieved, as well as obtained. The resulting chitin also has a high molecular weight that could not be obtained before, and there is no need to grind the raw material of the shell (cuticle) into fine powder, which facilitates separation of calcium carbonate and chitin that precipitate during the enzymatic treatment. This is a very important advantage in terms of production, and it was unthinkable with conventional enzyme treatment. Further, according to the present invention, the protein in the crustacean (cuticle) is recovered as an amino acid aqueous solution and used as a fertilizer, feed, food raw material, etc., while calcium carbonate is recovered in its natural form and used as a drug or the like. It opens the way to effective use. Furthermore, as an effect of the present invention, considerable calcium carbonate is precipitated by the enzyme treatment, so that the amount of acid used in the subsequent acid treatment of chitin can be reduced, so that it is not only economical, but also less modified by acid. can do.
Claims (6)
に放線菌由来のプロテアーゼであるアクチナーゼを作用
させることによって蛋白質及びカルシウムを除去分離し
た後、キチン中の残存カルシウムを酸処理により除去す
ることを特徴とする甲殻類の甲殻（クチクラ）の処理方
法。1. A crustacean (cuticle) of a crustacean such as shrimp and crab.
A method for treating crustacean crustaceans (cuticles), comprising removing actinase, a protease derived from actinomycetes, to remove proteins and calcium, and then removing residual calcium in chitin by acid treatment.
請求の範囲第１項記載の処理方法。2. The method according to claim 1, wherein actinase is allowed to act at a pH of 5 to 7.
第１項乃至第２項記載の処理方法。3. The processing method according to claim 1, wherein the processing temperature is 30 to 60 ° C.
として分離回収する特許請求の範囲第１項乃至第３項記
載の処理方法。4. The method according to claim 1, wherein the protein in the cuticle is separated and recovered as an amino acid solution.
過等物理的手段で沈殿として分離回収する特許請求の範
囲第１項乃至第３項記載の処理方法。5. The method according to claim 1, wherein calcium carbonate in the cuticle is separated and recovered as a precipitate by physical means such as filtration.
ラ四酢酸（以下、EDTA）によるものである特許請求の範
囲第１項乃至第３項記載の処理方法。6. The method according to claim 1, wherein the acid treatment is performed with hydrochloric acid or ethylenediaminetetratetraacetic acid (hereinafter referred to as EDTA).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1275330A JP2870871B2 (en) | 1989-10-23 | 1989-10-23 | A method for treating crustacean shells using enzymes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1275330A JP2870871B2 (en) | 1989-10-23 | 1989-10-23 | A method for treating crustacean shells using enzymes |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH03139291A JPH03139291A (en) | 1991-06-13 |
| JP2870871B2 true JP2870871B2 (en) | 1999-03-17 |
Family
ID=17553960
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1275330A Expired - Fee Related JP2870871B2 (en) | 1989-10-23 | 1989-10-23 | A method for treating crustacean shells using enzymes |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2870871B2 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1958618B (en) * | 2006-10-20 | 2010-10-06 | 宁波大学 | A kind of biological decalcification method in chitin preparation process |
| CN102170795B (en) * | 2008-09-12 | 2014-10-29 | 翡翠渔业公司 | Process for reducing the fluoride content when producing proteinaceous concentrates from krill |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10014997A1 (en) * | 2000-03-25 | 2001-09-27 | Cognis Deutschland Gmbh | Production of chitin comprises demineralizing and deproteinizing a chitin-containing material in the presence of a biodegradable complexing agent and a protease |
| JP4586178B2 (en) * | 2002-12-12 | 2010-11-24 | 三省製薬株式会社 | External preparation for improving skin damaged by burns |
| CN1891719B (en) | 2006-05-11 | 2010-05-12 | 江苏大学 | A kind of preparation method of chitin and chitosan of tortoise foot shell |
| US9814256B2 (en) | 2009-09-14 | 2017-11-14 | Rimfrost Technologies As | Method for processing crustaceans to produce low fluoride/low trimethyl amine products thereof |
| US8557297B2 (en) | 2008-09-12 | 2013-10-15 | Olympic Seafood, As | Method for processing crustaceans and products thereof |
| CN104045741B (en) * | 2013-09-29 | 2016-09-07 | 天津天狮生物发展有限公司 | A kind of chitosan preparation method |
| CN103535781B (en) * | 2013-10-23 | 2014-11-05 | 宁波大学 | Method for preparing instant shrimp leisure food |
| FR3031113B1 (en) * | 2014-12-31 | 2018-03-16 | Ynsect | PROCESS FOR PRODUCTION OF PRODUCT (S) OF INTEREST FROM INSECT BY ENZYMATIC HYDROLYSIS |
| GB2612617A (en) * | 2021-11-05 | 2023-05-10 | Marine Bioenergy As | Demineralisation of organic tissue |
-
1989
- 1989-10-23 JP JP1275330A patent/JP2870871B2/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1958618B (en) * | 2006-10-20 | 2010-10-06 | 宁波大学 | A kind of biological decalcification method in chitin preparation process |
| CN102170795B (en) * | 2008-09-12 | 2014-10-29 | 翡翠渔业公司 | Process for reducing the fluoride content when producing proteinaceous concentrates from krill |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH03139291A (en) | 1991-06-13 |
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