JP2879528B2 - Pack for simultaneous centrifugal analysis and measurement of blood components over multiple items - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a pack for simultaneous centrifugal analysis / measurement of a plurality of items of blood components, and more particularly, to a case where a pack is mounted on a centrifugal rotor and a plurality of blood components injected into the pack are mounted. The present invention relates to a pack having a feature of simultaneously analyzing and measuring chemical properties over various items.

[0002]

2. Description of the Related Art As a conventional pack for centrifugal analysis / measurement of blood components, for example, a pack shown in FIG. 9 is known. As is apparent from FIG. 9, a centrifugal rotor (not shown) is used. ), The pack is revolved many times while revolving the pack with a centrifugal rotor, and only one item of the chemical property of the blood component contained in the blood storage chamber provided in the pack is provided in the pack. In addition, analysis and measurement are performed using a mixed reagent accommodated in a single reagent mixing chamber by utilizing centrifugal action.

[0003]

However, in the case of the above-described conventional example, it is necessary to automatically rotate the pack itself mounted on the centrifugal rotor many times based on the revolution of the centrifugal rotor. In addition to the necessity of a centrifugal rotor with an extremely complicated mechanism, there is a problem that it is difficult to use the centrifugal rotor stably due to the occurrence of many troubles due to the complexity of the mechanism. Because only one item can be analyzed / measured for each pack, it is extremely inefficient and it is necessary to prepare and use many packs for analysis / measurement over many items. There's a problem.

The present invention has been developed and completed as a result of various studies with the object of solving the above-mentioned problems. It is possible to simultaneously analyze and measure the chemical properties of many items of blood components with a single pack. It is an object of the present invention to provide a pack for simultaneous centrifugal analysis / measurement of blood components over a plurality of items having extremely efficient and economical features.

[0005]

As means for solving the above-mentioned problems and achieving the object, the present invention provides a processing liquid storage section having a blood storage chamber and a plurality of reagent liquid storage chambers, and a processing liquid storage section having a blood storage chamber and a plurality of reagent liquid storage chambers. It is composed of a pack consisting of a reagent solution centrifugal movement processing unit and a component measurement unit having a plurality of measurement chambers.The blood storage chamber of the processing liquid storage unit has an opening for blood supply on the upper surface and an outer position at the outer position. Along with a blood outlet tube, each reagent solution storage chamber of the processing solution storage section has an opening for supplying each reagent solution on the upper surface, a siphon-type outlet tube for each reagent solution at the outer position, and a diluent solution at the inner position. The centrifugal movement processing unit includes a receiving port, a blood distribution channel, a plurality of blood centrifugal separation chambers connected to the distribution channel, and a reagent liquid in each of the reagent liquid storage chambers. A liquid receiving chamber is provided between each centrifugal separation chamber for each blood and each liquid receiving chamber. Are provided with suction pipes respectively, and liquid outlet pipes are respectively provided at positions outside the respective liquid receiving chambers, and the suction pipes and the liquid outlet pipes constitute suction devices, respectively. The leading end of the outlet pipe faces the distribution channel of the blood, and the tip of the siphon-type outlet pipe of the reagent liquid faces the receiving chambers, and the liquid outlet pipes of the receiving chambers face each other. The present invention provides a pack for simultaneous centrifugal analysis / measurement of blood components over a plurality of items, wherein the packs are configured so as to communicate with the respective measurement chambers of the component measurement section.

Further, according to the present invention, in the pack for simultaneous centrifugal analysis / measurement of a plurality of items of blood constituted as described above, the blood distribution channel is separated from the distal end position of the blood outlet tube by each blood separation chamber. And a pack for simultaneous centrifugal analysis / measurement of blood components over a plurality of items formed in a gentle curve with a downward slope toward the arrangement position.

[0007]

The operation of the pack for simultaneous centrifugal analysis / measurement of blood components over a plurality of items constituted as described above will be described together with the state of use. This pack is a centrifugal rotor R having the structure described in detail in the section of the embodiment. As shown in FIG. 7, the pack according to the present invention has the treatment liquid storage unit 1 directed toward the center of the inside of the rotor, the component measurement unit directed toward the outside of the rotor R, and 18a to 18e are fitted so as to match the positions of the respective through holes C provided in the peripheral portion of the rotor R.

Next, blood is stored in the blood storage chamber 4, and the reagent liquid storage chambers 5a to 5e are provided with reagent liquids for each item within a range not exceeding the siphon action line L shown in FIG. When the rotor R is rotated after containing the blood, the blood in the blood storage chamber 4 is centrifuged by the rotor R, and the blood distribution flow path 13 through the outlet pipe 8 provided outside (bottom) of the blood storage chamber 4. The blood flows through the inside and is injected into the centrifugal separation chambers 14a to 14e of the blood connected to the distribution channel 13, and the blood cells in the blood are separated from the plasma by the centrifugal force and sediment. At this time, since the reagent liquid in each of the reagent liquid storage chambers 5a to 5e is in an amount range not reaching the siphon action line L, each of the reagent liquid storage chambers 5a to 5e is not subjected to the siphon action.
Is reserved within.

In this state, when a diluting liquid is supplied from an injection pipe, which will be described later, to each of the diluting liquid receiving ports 12a to 12e provided inside the processing liquid storage unit 1, the diluting liquid is centrifuged by the rotor R. The liquid is supplied into the reagent liquid storage chambers 5a to 5e, and the amount of liquid in each storage chamber increases to exceed the siphon action line L.

At the same time as passing the siphon action line L, the reagent solution in each of the reagent solution accommodating chambers 5a to 5e is transferred by siphon action to each of the liquid receiving chambers 15a to 15e via each of the siphon type outlet pipes 11a to 11e. The respective liquid receiving chambers 15a to 15e and the respective centrifugal separation chambers 14a to 14e for blood.
Each of the suction pipes 16a to 16e disposed therebetween and each of the liquid receiving chambers 1
5a to 15e, each liquid outlet pipe 17a disposed at an outer position
The separated plasma located in the upper layer of the centrifugal separation chamber of each blood is centrifuged together with the diluted reagent liquid through each of the liquid suction pipes 17a to 17e through the respective suction devices AS constituted by the liquid suction pipes 17a to 17e. Through each of the measurement chambers 18a-
18e, are mixed in each of the measurement chambers, and simultaneously perform a desired centrifugal analysis / measurement of a plurality of items of a blood component by detecting, for example, a change in absorption of light emitted from the light emitter with a detector. Things.

[0011]

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a perspective view of a pack for simultaneous centrifugal analysis and measurement of blood components according to the present invention. A processing liquid storage unit 1 that stores blood and a reagent liquid disposed at a (center side) position;
Of the blood and reagent liquid centrifugal movement processing unit 2 connected to the outside position of the component, and the component measurement unit 3 connected to the outside position of the centrifugal movement processing unit 2, namely, three divided bodies, It is composed of an intermediate portion and an outer portion, which are integrally combined to form a single dividable pack, and the whole is made of a transparent plastic material.

In this embodiment, the processing liquid storage unit 1 has one blood storage chamber 4 provided on one side and five reagents in this embodiment which are arranged in parallel from the blood storage chamber 4 to the other side. The liquid storage chambers 5a, 5b, 5c, 5d, and 5e, and a filter chamber 6 provided on the other side of each of the reagent liquid storage chambers are provided. A blood outlet pipe 8 is provided at an outer position (bottom), and an air moving pipe 9 is provided at an inner position. The distal ends of the blood outlet pipe 8 and the air moving pipe 9 are connected to a blood distribution flow described later. Each is facing the street.

Each of the above-mentioned reagent liquid storage chambers 5a, 5b, 5
c, 5d, and 5e are openings 10a for supplying a reagent liquid on the upper surface,
10b, 10c, 10d, and 10e, and each of the siphon-type outlet tubes 11a, 11
b, 11c, 11d, and 11e, and diluent receiving ports 12a, 12b, 12c, 12d, and 12e at the inner positions, respectively, and the tip of each siphon-type outlet pipe faces a liquid receiving chamber for a reagent liquid described later. There is not. In FIG. 1,
The symbol L indicates a siphon action line, and when the amount of the reagent liquid in each reagent liquid storage chamber exceeds this line L, the reagent liquid is discharged to each liquid receiving chamber via each siphon type discharge pipe. Is configured.

The blood and reagent liquid centrifugal movement processing unit 2 further includes a blood distribution flow path 13 and a blood flow path corresponding to the reagent liquid storage chambers 5a to 5e provided in communication with the distribution flow path. Centrifuge chambers 14a, 14b, 14c, 14d, 14
e, and a reagent liquid receiving chamber 1 in each of the reagent liquid storage chambers 5a to 5e.
5a, 15b, 15c, 15d, and 15e, and a storage chamber 16 for surplus blood.
Is formed in a gentle curve with a downward slope from the distal end position of the blood outlet tube 8 toward the position where the centrifugal separation chambers 14a to 14e for each blood are disposed.

Further, the centrifugation chambers 14a-1
Suction pipes 16a, 16b, 16c, 16d, and 16e are provided between 4e and the receiving chambers 15a to 15e for the respective reagent solutions, and blood in the centrifuged chambers 14a to 14e is centrifuged. It is configured to suck the plasma into the liquid receiving chambers 15a to 15e.

Further, the liquid outlet pipes 17a, 17b, 17c are located at positions (bottoms) outside the liquid receiving chambers 15a to 15e.
17d and 17e are provided respectively, and as clearly shown in FIG. 4, the tips of the suction pipes 16a to 16e on the liquid receiving chambers 15a to 15e side are slightly set in the liquid outlet pipes 17a to 17e. The suction device AS is configured to be loosely inserted, and the respective leading ends of the liquid outlet pipes 17a to 17e are connected to the measurement chambers 18a and 18 formed of the transparent material of the blood component measurement unit 3 described above.
b, 18c, 18d, and 18e.

The processing liquid storage unit 1, the centrifugal movement processing unit 2, and the component measuring unit 3 configured as described above are combined by combining the three-pieces shown in FIGS. ,
As shown in FIGS. 1 and 2, each of the distal ends of the blood outlet pipe 8 and the air moving pipe 9 in the blood storage chamber 4 of the processing liquid storage unit 1 is centrifugally moved. The distal end of each of the siphon type outlet pipes 11a to 11e of each of the reagent liquid storage chambers 5a to 5e faces each of the liquid receiving chambers 15a to 15e of the centrifugal movement processing unit 2. Further, the distal end of the exhaust pipe 6 ′ of the filter chamber 6 of the processing liquid storage unit 1 is connected to the blood distribution channel 1 of the centrifugal movement processing unit 2.
3 facing the upper part.

Each liquid receiving chamber 15a of the centrifugal movement processing unit 2
The leading ends of the liquid outlet pipes 17a to 17e of the blood component measuring sections 18a to 18e are located above the measuring chambers 18a to 18e of the blood component measuring section 3 in this manner. This is an example of a measurement pack.

Next, the use state of the above embodiment will be described together with the operation and effects. This pack is used, for example, mounted and fixed on the upper surface of a centrifugal rotor R having a structure schematically shown in FIG. And the centrifugal rotor R is
On the upper surface of the central part, a cylindrical diluting liquid distributor D in which ring-shaped diluting liquid injection parts a, b, c, d, e. , Diluent injection pipes a ₁ , b ₁ , c ₁ , d ₁ , e _1, ... Are arranged outwardly, and the detector B receives light emitted from the projector A at the periphery of the rotor. And a plurality of through-holes C are formed, and the motor M rotates at high speed.

Further, outside the rotor R, diluting liquid injection pumps P ₁ , P ₂ , P ₃ , P ₄ , P ₅ ... Are provided.
Each pump has a diluent supply pipe a ₂ , b ₂ , c ₂ , d ₂ , e ₂ .
Are connected to the diluting liquid injection parts a, b, c, d, e,.

Next, about 300 μl of human blood to which blood has been collected and to which peppera has been added is injected into the blood storage chamber 4 through the opening 7, the opening 7 is appropriately sealed, and each of the reagent liquid storage chambers 5 a to 5 e. LDH, GOT, G by inspection measurement item in
150 μl of a quantitative reagent such as PT was injected from the openings 10a to 10e at a concentration twice or more the required concentration. At this time, it should be noted that, when the centrifugal force acts, each amount of the reagent solution to be injected into each of the storage chambers 5a to 5e is within a range not exceeding the siphon action line L shown in FIG. .

Next, this pack is placed on the upper surface of the rotor R configured as described above, and the processing liquid storage section 1 is directed toward the center inside the rotor as shown in FIG.
The component measuring section 3 faces the outside of the rotor R, and the measuring chambers 18a to 18e are mounted and fixed so as to match the positions of the through holes C of the rotor R. dilution infusion tube a ₁ of the rotor R side receptacle 12a~12e of, b _1, c _1, after inserting the d ₁ ... tip of rotating the centrifuge rotor R at about 2000RPM drives the motor M.

When the rotor R rotates, the liquid surface of each reagent solution in each of the reagent solution storage chambers 5a to 5e faces the rotation center side of the rotor due to the centrifugal action of the rotor R.
In addition, since the liquid surface is located outside (below) the siphon action line L, no siphon action is performed, and each reagent solution is reserved in each of the reagent solution storage chambers 5a to 5e.

On the other hand, the blood in the blood storage chamber 4 is
The blood flows into the blood distribution channel 13 through the blood outlet tube 8 due to the rotational centrifugal force, and flows into each of the centrifugal separation chambers 14a to 14e that are connected and communicated along the distribution channel 13. Because the flow path 13 is formed in a gentle curve with a downward slope, the flow smoothly flows toward the larger centrifugal force and fills all the centrifugal separation chambers 14a to 14e.
Are stored in a reservation room 16 provided at the terminal of the first embodiment. At this time, with the transfer of the blood in the blood storage chamber 4, the air in the pack is transferred into the blood storage chamber 4 through the air moving pipe 9, and in this state, the blood flows for about 100 seconds. The blood in each of the centrifugation chambers 14a to 14e is separated into sedimented blood cells and upper layer plasma. In addition, for example, the above-mentioned reagent solution LDH is composed of nicotinamide dinucleotide (0.001M) and pyrophoric acid (0.001M).
Is contained in a pyrophosphate buffer (pH 8.3, 0.1M), and nicotinamide dinucleotide is reduced by the action of plasma lactate dehydrogenase.

[0025] After performing the centrifugal separation process of about 100 seconds, the respective infusion pump P ₁ each reagent from to P ₄ liquid accommodating chamber 5a
To exert a siphon type outlet pipe 11a~11e provided in ～5E, when injecting 150μl of water as dilution, the dilution in each injecting section a of the supply pipe a ₂ to e ₂ and the distributor D, b, c, d, e, and each of the injection pipes a _{1 to} e _1, and are additionally injected into each of the reagent liquid storage chambers 5 a to ₅ d through each of the receiving ports _{12 a} to 12 e provided in the processing liquid storage unit 1.

When the dilution liquid is additionally injected, the liquid volume in each of the reagent liquid storage chambers 5a to 5e is increased to 300 μl, and each liquid surface position exceeds the siphon action line L, and at the same time, each reagent liquid storage chamber 5a The reagent liquids in the liquid chambers 5a to 5e move in the centrifugal direction by the siphon action of the siphon-type outlet pipes 11a to 11e and are drawn out to the liquid receiving chambers 15a to 15e. At this point, the air pressure in the pack is higher than the external pressure in accordance with the amount of liquid discharged from the siphon-type discharge pipe, so that the air in the pack is sent to the filter chamber 6 via the exhaust pipe 6 ', After filtering in the chamber 6, it is released outside the pack.

On the other hand, each siphon-type outlet pipe 11a-11
When the reagent solution is drawn out from the e to each of the liquid receiving chambers 15a to 15e, each of the suction pipes 16a to 16e arranged between the centrifugal separation chambers 14a to 14e and each of the liquid receiving chambers 15a to 15e.
15e each liquid outlet pipe 17a-17 arranged outside
e, about 5 .mu.l of plasma in each of the centrifugal separation chambers 14a to 14e is sucked into each of the liquid receiving chambers 15a to 15e by the suction action of each of the liquid receiving chambers 15a to 15e.
The liquid flows into the measuring chambers 18a to 18e of the component measuring section 3 via the liquid outlet pipes 17a to 17e together with the reagent liquid in 5e, and is mixed.

The chemical reaction of each mixed solution in each of the measurement chambers 18a to 18e, that is, the chemical reaction between the reagent solution such as LDH, GOT, GPT and the plasma and the plasma, was performed by emitting light from the light emitting device A to the rotor R. The light is received by the detector B through each through-hole C and each measurement chamber, and is measured by detecting the temporal absorption change of the light by the detector B. Specifically, for example, the activity of lactate dehydrogenase in plasma in a mixture of 300 μl of a reagent solution LDH diluted with water and 5 μl of separated and separated plasma is determined by the nicotinamide contained in the reagent solution.ヂ The reduction rate of nucleotides can be detected and measured by the detector B based on a change in absorption of light of 340 nm emitted from the projector A.

After the measurement is completed as described above, the rotation of the centrifugal rotor R is stopped, the pack is removed from the rotor and immersed in a disinfecting solution, and then washed and regenerated to perform one cycle of centrifugal analysis and measurement of blood components. Is to complete.

As is clear from the above description, according to this embodiment, centrifugal analysis / measurement of blood components can be performed efficiently over a large number of items with one pack, and packs can be formed by rotating a centrifugal rotor. Since the analysis and measurement can be performed only by the revolution of the pack, and the rotation of the pack is not required, there is a great advantage that the centrifugal rotor having a simple configuration can be easily operated.
With the unique configuration and operation of the embodiment including the shape of 3, the intended analysis / measurement can be performed quickly, smoothly and accurately, and the operation can be performed in a substantially completely sealed state. Thus, centrifugal analysis and measurement of blood components can be achieved completely and hygienically without adversely affecting the environment.

Although the main embodiments of the present invention have been described in detail, the present invention is not limited to the above-described embodiments, but can achieve the object of the present invention and do not depart from the gist of the invention. Various design changes are possible.

[0032]

Since the present invention is constructed as described above and used as described above, the following useful remarks which cannot be achieved by the above-mentioned conventional pack for centrifugal analysis and measurement of blood components. Effects can be achieved.

(1) Since chemical properties of blood components over a wide range of items can be simultaneously analyzed and measured by a single pack by centrifugation, it is extremely efficient and economical. (2) The desired analysis / measurement can be performed only by revolving the pack by rotation of the centrifugal rotor, and there is no need to rotate the pack on the centrifugal rotor. Can be operated. (3) Blood and reagent liquids are moved and mixed using the principle of siphon and the principle of suction by a suction device, so their actions are very stable, and the intended analysis and measurement are performed smoothly and accurately. Can be achieved.

[Brief description of the drawings]

FIG. 1 is a plan view showing an embodiment of the present invention.

FIG. 2 is a vertical sectional side view of the main part.

FIG. 3 is a side view of the blood storage chamber.

FIG. 4 is an enlarged vertical sectional view of the suction device.

FIG. 5 is an exploded plan view of the pack.

FIG. 6 is a longitudinal sectional side view of a main part in a disassembled state.

FIG. 7 is a longitudinal sectional side view of a main part in a state of being mounted on the upper surface of the centrifugal rotor.

FIG. 8 is an explanatory diagram of a state of injection of a diluting liquid.

FIG. 9 is an explanatory view of a conventional centrifugal analysis / measurement pack for blood component rotating on a centrifugal rotor.

[Explanation of symbols]

DESCRIPTION OF SYMBOLS 1 Processing liquid storage part 2 Centrifugal movement processing part 3 Component measurement part 4 Blood storage chamber 5a, 5b, 5c, 5e Reagent liquid storage chamber 6 Filter chamber 6 'Exhaust pipe 7 Opening 8 Blood lead-out pipe 9 Air transfer pipe 10a, 10b, 10c, 10d, 10e Opening 11a, 11b, 11c, 11d, 11e Siphon-type outlet pipe 12a, 12b, 12c, 12d, 12e Receptacle L Siphon working line 13 Blood distribution flow path 14a, 14b, 14c, 14d, 14e Blood centrifugation chamber 15a, 15b, 15c, 15d, 15e Liquid receiving chamber 16 Excess blood retaining chamber 16a, 16b, 16c, 16d, 16e Suction pipe 17a, 17b, 17c, 17d, 17e Liquid outlet pipe 18a, 18b, 18c, 18d, 18e Measurement chamber AS aspirator R centrifugal rotor a, b, c, d ... Dilution liquid injection part D Dilution distributor a _{1 of, b 1, c 1, d} 1 ... dilution infusion tube solution _{a 2, b 2, c 2} , d 2 ... feed pipe P ₁ of _{dilution, P 2, P 3, P} 4 … Diluent injection pump C Through hole M Motor A Floodlight B Detector

──────────────────────────────────────────────────続 き Continuation of the front page (56) References JP-A-61-147469 (JP, A) JP-A-62-115144 (JP, U) Tokuhyo Hei 5-508709 (JP, A) International publication 93/16391 (WO, A1) (58) Field surveyed (Int. Cl. ⁶ , DB name) G01N 33/48 G01N 33/49

Claims (2)

(57) [Claims] 1. A pack comprising a processing liquid storage section having a blood storage chamber and a plurality of reagent liquid storage chambers, a centrifugal movement processing section for blood and each reagent liquid, and a component measurement section having a plurality of measurement chambers. The blood storage chamber of the processing liquid storage section has an opening for blood supply on the upper surface, and a blood outlet pipe at an outer position, and each reagent liquid storage chamber of the processing liquid storage section has An opening for supplying the reagent solution, a siphon-type outlet pipe for each reagent solution at the outer position,
A diluent receiving port is provided at an inner position, and the centrifugal movement processing unit includes a blood distribution channel, a plurality of blood centrifugal separation chambers respectively connected to the distribution channel, and the reagent solution. Along with each of the receiving chambers for the reagent solution in the storage chamber,
Suction pipes are respectively arranged between the centrifugation chamber of each blood and each liquid receiving chamber, and liquid outlet pipes are respectively arranged outside the liquid receiving chambers. Each of the liquid outlet pipes constitutes an aspirator, and the tip of the blood outlet pipe faces the blood distribution flow path, and the tip of the siphon type outlet pipe for each reagent liquid is connected to each of the liquid receiving chambers. For simultaneous centrifugal analysis / measurement of blood components over a plurality of items, wherein each liquid outlet pipe of each liquid receiving chamber is connected to each measuring chamber of the component measuring unit. pack. 2. The plurality of blood distribution channels according to claim 1, wherein the blood distribution flow path is formed in a gentle curve with a downward slope from the distal end position of the blood outlet pipe toward the arrangement position of each blood separation chamber. Pack for simultaneous centrifugal analysis / measurement of blood components across a range of items.