JP2882671B2 - Cosmetics - Google Patents

Description

Description: BACKGROUND OF THE INVENTION The present invention relates to cosmetics, and more particularly, to cosmetics having an effect of improving skin conditions, a hair nourishing effect, and a white hair improving effect.

[Conventional technology]

2. Description of the Related Art Conventionally, moisturizers, extracts of crude drugs, proteins, and extracts of organs have been known as cosmetic ingredients having an effect of improving the skin condition.

Extracts of crude drugs, hormones, vitamins, and the like are known as cosmetic ingredients having a hair nourishing effect on hair.

In addition, as a cosmetic ingredient having an effect of improving white hair, an extract of crude drug, hormones, vitamins and the like are known.

[Problems to be solved by the invention]

By the way, conventional cosmetic ingredients having an effect of improving skin condition, cosmetic ingredients having a hair nourishing effect, and cosmetic ingredients having an effect of improving white hair are inferior in effect or show a certain effect even if they exhibit a certain effect. And the skin condition is deteriorated.

The present invention solves such a conventional problem,
It is a technical problem to provide a cosmetic composition comprising a safe cosmetic ingredient that has an effect of preventing hair loss or improving gray hair to black hair without deteriorating the skin condition and has almost no side effects. And

[Means for solving the problem]

In order to solve the above-mentioned problems, the cosmetic of the present invention is obtained by mixing a urine collected from mammals with a cosmetic ingredient obtained by performing a deammonification treatment and a sterilization treatment. It is.

 Hereinafter, the present invention will be described in more detail.

The mammals used for collecting urine of the present invention include humans, monkeys, horses, cows, bears, dogs, cats, and the like.

The present invention is a cosmetic comprising a cosmetic ingredient (hereinafter referred to as adjusted urine) obtained by subjecting urine collected from these mammals to deammonification treatment and sterilization treatment. .

The deammonia treatment temperature of the collected urine is preferably room temperature. When the treatment temperature is 50 ° C. or higher, it is not preferable because the active ingredient mixed in the collected urine is decomposed. When deammonifying the collected urine from the liquid phase, for example, an ammonia capture column method may be used.

The sterilization treatment may be performed, for example, by filtering and sterilizing the collected urine using a filtration sterilization filter.

According to the method as described above, liquid adjusted urine can be obtained.

Furthermore, the so-called freeze-drying method, in which the collected urine is deammonified and sterilized by the above-described method and then frozen, and then dried under reduced pressure from a low-temperature solid state, is used. It is preferable because it is possible to obtain a state-adjusted urine.

When this powder adjusted urine is dissolved again in a solvent such as water, liquid adjusted urine can be obtained.

The adjusted urine thus obtained in liquid or powder form can be used as a cosmetic base. Moreover,
It is preferable to use a pH adjuster such as sodium bicarbonate or a good buffer in combination with the adjusted urine thus obtained, since the hydrogen ion concentration is stabilized. in this case,
The pH is adjusted with hydrochloric acid or sodium hydroxide, but the pH is preferably 6 to 8 in view of the stability of the adjusted urine.

Such a modified urine obtained in a liquid or powder form can be adjusted to a desired concentration and mixed with other cosmetic ingredients to form the cosmetic of the present invention.

The amount of the adjusted urine used in the present invention as described above can be 0.005 to 90% by weight of the total cosmetics including other cosmetic ingredients, but in consideration of viscosity, feel to the skin, and the like, 0.005 to 40% by weight is desirable.

When the amount is less than 0.005% by weight, a remarkable cell growth effect cannot be confirmed, which is not preferable.

The cosmetic of the present invention can be obtained by combining the above-mentioned adjusted urine with the following cosmetic components within a range that does not impair the object of the present invention.

In the cosmetic of the present invention, specific examples of the cosmetic ingredient to be mixed with the adjusted urine include, for example, hydrocarbons, waxes,
Bases such as oils and fats, esters, higher fatty acids, higher alcohols, and surfactants, and additional components such as fragrances, pigments, preservatives, antioxidants, ultraviolet protective agents, alcohols, and medicinal ingredients can be given. .

Specific examples of the cosmetic of the present invention, which is prepared by mixing the adjusted urine with the above-mentioned cosmetic ingredients, include, for example, emollient cream, hair lotion, milky lotion, hair cream, ointment and the like. However, the scope of the cosmetic of the present invention is not limited to these.

(Example of adjusted urine production)

 Hereinafter, a production example of the adjusted urine used in the present invention will be described.

In practicing the present invention, adjusted urine 1 to 5 are first manufactured as described below, effect experiments are performed using a part of each, and then the remaining adjusted urine 1 to 5 is prepared using the remaining adjusted urine 1 to 5. The cosmetics of the invention were prepared and the effects of the examples were confirmed.

<Preparation Example 1 of Adjusted Urine> 50 cc of urine was collected from each of 10 humans, mixed with each other, and subjected to a deammonification treatment using an ammonia capture column. The conditions in the ammonia capture column were room temperature.

Next, the deammonia collected urine was sterilized by filtration under room temperature conditions to obtain a cosmetic base material.

<Adjusted urine production example 2> The procedure was the same as that of Adjusted urine production example 1 except that 10 cc of urine was collected from each of 10 monkeys.

<Adjusted urine production example 3> The same procedure was performed as in adjusted urine production example 1 except that 10 cc of urine was collected from each of 10 horses.

<Adjusted urine production example 4> The procedure was the same as that of Adjusted urine production example 1 except that 50 cc of urine was collected from each of 10 cows.

<Adjusted urine production example 5> The procedure was the same as that of Adjusted urine production example 1 except that 10 cc of urine was collected from each of 10 dogs.

<Cell proliferation effect assay> Human skin fibroblasts, human skin keratinocytes,
A cell proliferation effect assay test was performed on each of the human hair follicle keratinocytes.

A Cell growth promotion test using fibroblasts Culture target Human skin fibroblasts Substrate 60 mmφ commercial culture dish Cell culture medium Minimum essential medium (MEM) is fetal bovine serum (F
A mixed medium containing 1% by volume of BS) Gas phase conditions Air mixed with 5% by volume of carbon dioxide Temperature 37 ° C Under the above conditions, 100,000 fibroblasts of 6 million each were grown.
A total of 600,000 pieces were put into each culture dish. Next, the cosmetic ingredients of Adjusted Urine Production Examples 1 to 3 were added to four of the six culture dishes in amounts shown in Table 1, respectively. In addition, one culture dish to which no cosmetic base was added was used as a control, and the other culture dish was added with a conventional hair growth cosmetic base, vitamin E, as a comparative example.

After the addition of the cosmetic ingredients, a calculated amount of salts was previously added to each cosmetic ingredient so that the salt concentration of the added culture dish did not deviate from the physiological salt concentration.

After culturing for 96 hours, the cultured cells in each culture dish were detached with trypsin, and the number of cells was counted with a hemocytometer.

The results are shown in Table 1 assuming that the number of cells obtained in the control was 100%.

B Cell proliferation promotion test using epidermal keratinocytes Culture target Human skin epidermal keratinocytes Substrate Type IV collagen is applied to a commercial culture dish of 60 mmφ Cell culture medium The minimum essential medium (MEM) is fetal bovine serum (F)
BS) was mixed at 1% by volume and the calcium concentration was further adjusted to 0.3 mM. The medium other than the above-mentioned culture target, substrate and cell culture medium was the same as the fibroblast culture method.

Table 2 shows the types and amounts of the cosmetic ingredients used, and the measurement results.

C Cell proliferation promotion test using hair follicle keratinocytes Culture target Human hair follicle keratinocytes Other than the culture target, the human skin keratinocyte culture method was followed.

Table 3 shows the types and amounts of the cosmetic ingredients used and the measurement results.

From Tables 1 to 3, it can be seen that when the cosmetic ingredients of each of the adjusted urine production examples were added to the cell culture system, there was a remarkable cell growth promoting effect, and the cosmetic ingredients used in the present invention were skin or hair follicles. Was found to have an activating effect on the cells constituting

<Black hair follicle test> A black hair test was performed using mouse hair follicles.

Test subjects Hair follicles from Ay mice Cell culture medium Fetal bovine serum (F)
About 100 hair follicles to be tested and 5 cc of cell culture medium are combined into each of four test tubes, and 2 of them are mixed.
In one test tube, the cosmetic base material of Preparation Urine Production Example 1 was used, and in one test tube, a conventional hair growth cosmetic base material Vitamin E was used as a comparative example.
Each was added in the amounts shown in Table 4. The remaining one test tube was used as a control.

Adjustment of the gas-phase conditions and temperature, and further, the salt content were the same as in the case of the hair growth effect test.

 Rotary culture was performed for 2 days.

 Table 4 shows the results.

From the above results, it was confirmed that each adjusted urine production example had an effect of acting on melanocytes of hair follicles to blacken hair follicles.

<Hair-growth effect test> A hair-growth effect test was performed using the back of 10 mice.

That is, 70 C3H mice 9 weeks old (1 for each sample application group)
The hair of each of the 0) was shaved to a size of 2.0 × 2.0 cm, and the following day, each of the adjusted urine production examples 1,2,4,5, and ethanol, water as a control, and As a comparative example, vitamin E, which is a conventional hair growth effect cosmetic ingredient,
Each was applied.

On the 5th and 14th days, the density of hair growth at the site was measured using a color difference meter, and the result was taken as the degree of black increase.

 Table 5 shows the results.

These results indicate that the adjusted urine has a mouse hair growth promoting effect.

〔Example〕

Hereinafter, the present invention will be described with reference to Examples and Comparative Examples, but the present invention is not limited to these Examples.

 In addition, the compounding amount is a weight part.

<Example 1> An emollient cream was prepared according to the formulation shown in Table 6.

The above formulation A is mixed and heated to 70 ° C. The above formulation B was similarly mixed and heated to a temperature of 70 ° C., and was uniformly emulsified with a homomixer. When cooled to 40 ° C., the above C was added and cooled again. An emollient cream which is one embodiment of the invention is obtained.

<Example 2> A lotion was prepared according to the formulation shown in Table 7.

The above formulations A and B are heated and dissolved, A is added to B and solubilized to obtain a lotion according to one embodiment of the present invention.

<Product effect test><< Moisturizing effect test >> In winter, the above Example 1 was applied to the entire back of the right hand of a panel of 40 men and women with dry or rough skin, and the same amount of purified water was used instead of the adjusted urine of Example 1. A control product similar to that of Example 1 was applied to the back of the left hand except that it was used.

After 4 hours, the water status of the left and right skin was evaluated by impedance measurement.

As a result, within the same individual, the impedance value of the right instep was determined by the student's paired t test.
It showed a high value with a significant difference of 5% or less as compared with the left upper, indicating that it had a moisturizing effect.

<< Skin condition improvement effect test >> To the same panelists who performed the moisturizing effect test, the lotion of Example 2 was applied to the back of the right hand three times daily for 30 days. A control product similar to that of Example 2 except that purified water was used instead of the adjusted urine of Example 2 was applied to the back of the left hand.

Thirty days later, as a sensory evaluation item, the degree of improvement of the bulkiness of the skin was evaluated based on the scores in Table 8, and the average value of 40 persons was obtained.

 Table 9 shows the results.

As is clear from Table 9, the product of the present invention was found to have an effect of improving the bulkiness of the skin.

Example 3 A hair lotion was prepared according to the formulation shown in Table 10.

The above formulation is dissolved by heating and solubilized to obtain a hair lotion which is one embodiment of the present invention.

<< Hair-growth effect test >> A hair-growth effect test was carried out by using the hair lotion shown in Example 3 to 13 panelists complaining of hair thinning. That is, in the same manner as a normal lotion usage for five consecutive months, the head was evenly applied twice a day.

The evaluation was performed three and five months after the start of use. Table 11 shows the results.

At the end of this test, there were four people who reported reduction in gray hair. In addition, the condition of the scalp was also improved, and there were 7 people who reported reduction in the occurrence of dandruff.

Example 4 An emulsion was prepared according to the formulation shown in Table 12.

The above formulation A is mixed and heated to 70 ° C. The above formulation B was similarly mixed and heated to a temperature of 70 ° C., and was uniformly emulsified with a homomixer. When cooled to 40 ° C., the above C was added and cooled again. An emulsion is obtained which is one embodiment of the invention.

Example 5 A hair cream was prepared according to the formulation shown in Table 13.

The above formulation A is mixed and heated to 80 ° C. In the same manner, the above formulation B was mixed and heated to 80 ° C., and the mixture was uniformly emulsified with a homomixer. When cooled to 40 ° C., the above C was added and cooled again. To obtain a hair cream which is one embodiment of the present invention.

〔The invention's effect〕

The following effects were observed in the adjusted urine. That is, human skin fibroblasts, human skin epidermal keratinocytes, and human hair follicle keratinocytes had a cell growth effect, a hair follicle blackening effect, and a hair nourishing effect. From this, when the adjusted urine is used as a cosmetic ingredient, the cosmetics containing it have a cell growth effect on human skin fibroblasts, human skin keratinocytes, and human hair follicle keratinocytes, and a hair follicle blackening effect. It was also found that the hair growth effect was recognized.

Therefore, the cosmetic of the present invention has a cell growth effect, a hair follicle blackening effect and a hair nourishing effect on human skin fibroblasts, human skin keratinocytes, and human hair follicle keratinocytes.

In addition, the cosmetic of the present invention has an effect of improving the bulkiness of the skin, preventing hair loss or returning gray hair to black hair, and is safe with almost no side effects.

──────────────────────────────────────────────────続 き Continuing on the front page (72) Inventor Yukiko Yokoyama 27-1 Takashimadai, Kanagawa-ku, Kanagawa-ku, Yokohama, Japan Inside the Polar Chemical Industry Co., Ltd. Yokohama Research Laboratory (72) Inventor Masami Suzuki 27-27 Takashimadai, Kanagawa-ku, Yokohama, Kanagawa 1 Pola Kasei Kogyo Co., Ltd. Yokohama Research Laboratory (58) Field surveyed (Int. Cl. ⁶ , DB name) A61K 7/00, 7/06

Claims (2)

(57) [Claims] (1) A cosmetic composition comprising a urine collected from mammals and a cosmetic ingredient obtained by subjecting the urine to deammonification treatment and sterilization treatment. 2. The cosmetic according to claim 1, wherein the cosmetic base is in a powder form.