JP2887580B2 - Method and apparatus for measuring optically active state of platelet preparation - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION The present invention relates to a platelet preparation obtained by separating platelets isolated from whole blood (red blood cells, white blood cells, platelets, and plasma) or platelets collected by blood component collection in plasma, which are transfused after production. Before being used for whether or not the effect of blood transfusion has not been expected due to the passage of the storage days, for the decrease in the activity of the platelets, to provide a measure of the degree of optical quality for suitable quality confirmation A measuring method for the active state,
The present invention relates to a measurement device that can be used for performing the method.

[0002]

2. Description of the Related Art Currently, platelets, which are one of blood products, have an expiration date of less than 3 days in Japan. This is because, in the case of this kind of preparation, the activity of platelets decreases as the number of storage days increases. In this case, if the storage condition is poor, the activity decreases, Even within, the effect of blood transfusion cannot be expected. Therefore, for platelet preparations,
After confirming its active state, it is indispensable to use it for blood transfusion and the like, and various proposals have already been made for the confirmation method.

[0003] That is, since the quality of a platelet preparation has a strong correlation with the pH of its contents, measurement of pH can be a means of judging a decrease in activity. For this purpose, a blood bag containing a platelet preparation must be used. A sample must be withdrawn, and therefore a needle must be inserted into an aseptically manufactured blood product, which is not a suitable method for practical use. In recent years, research on platelet activity has progressed, and it has a correlation with the morphological change of platelets themselves.
It has been known that platelet having a morphological form has high activity, and when it changes to a spherical shape or a spherical shape with pseudopodia, its activity decreases. Therefore, since the activity of the platelet preparation can be indirectly promoted by measuring this morphological change, a device capable of optically detecting the morphology of platelets in a blood bag without using a needle or the like is also disclosed in 1980. It has been in development since around the year.

[0004] Bellhouse et al. Disclose an optical measurement method for stored human platelets (Japanese Patent Laid-Open No. 60-113153) for nondestructively monitoring the survival rate of stored platelets in a platelet preparation stored in a blood bag. Such proposals are disclosed. That is, the above proposal focuses on a phenomenon in which, when a platelet preparation filled with normal disk-shaped platelets is swung over light, scattered light appears to fluctuate due to the rotation of the disk-shaped platelets, and this is considered by an expert. It is assumed that the fluctuation amplitude of scattered light accompanied by small fluctuations from platelets flowing in a specific direction may be measured instead of visual determination by the method described above, and the fluctuation amplitudes may be measured in one day when platelets are fresh. It is substantially constant between positions, but gradually decreases to almost zero as platelets die, and in this case, the average amplitude of the scattered light varies depending on the sample, but in any case, The damping of the fluctuation amplitude is based on a similar curve.

[0005] The technical content of the above proposal is to divide a blood bag into two parts, upper and lower, through a flow path having a predetermined thickness, and to allow the blood platelet to flow back and forth in the flow path. By oscillating the bag and irradiating the above-mentioned flow path with light rays, the fluctuation amplitude of the intensity of the scattered light emitted from the platelets in a specific direction is detected, and the detected value is separately determined for fresh platelets as described above. This is compared with a reference value of the fluctuation amplitude detected below.

[0006]

However, in the conventional method for measuring the fluctuation amplitude of the scattered light from platelets as described above, the measurement is performed while the blood bag is vertically suspended. When storing the blood bag, the blood bag must be held under the measurement conditions different from the storage conditions in which the blood bag is most desirably placed in the laterally mounted state and swung laterally. Not only does it harm the blood bag, but also it is not possible to use a horizontal shaker for preservation during measurement, and the plunger is moved by a cam driven at a constant rotation speed by an electric motor, which presses the blood bag against the force of the spring. By doing so, the platelet preparation must flow vertically through the channel. Furthermore, since the fluctuation amplitude of the scattered light by the platelets is used as described above, it is difficult to sufficiently improve the sensitivity.

[0007] Further, in the case of the above-mentioned conventional example, the thickness of the flow path must be made constant as described above. For this reason, the blood bag is sandwiched so as to have a predetermined constant thickness. Therefore, since the sheet thickness of the blood bag actually used has various dimensions, the thickness of the flow path changes depending on the size of the sheet thickness, and it is difficult to obtain a highly reliable measurement result.

[0008] The present invention is the same in that a normal platelet ratio (% DISK) in a blood bag is measured by using a non-puncture needle, instead of utilizing the fluctuation amplitude itself of scattered light by platelets. Focusing on the light transmittance of the platelet preparation of (1), if the thickness of the platelet preparation in the flow path to be measured is constant and the flow rate of the preparation flowing through the flow path is also constant, the blood bag is stationary. The rate of change in light transmission intensity, which is determined from the static light transmission intensity when in the state and the dynamic light transmission intensity when the platelet preparation is flowing in a direction perpendicular to the optical axis, is correlated with% DISK. This is based on recognition.

[0009] In view of the above-mentioned conventional problems, a first aspect of the present invention is described.
In the optical activity state measurement method according to the above, the blood bag is in a horizontal state, and is sandwiched between flat plates, so that the blood bag is divided into two reservoirs via a platelet preparation layer for measurement, which is a flow path of a predetermined thickness. Then, in the stationary state of the blood bag, irradiate light beams in the orthogonal direction, measure the transmitted light of the platelet preparation layer for measurement to obtain a static light transmission intensity, and move the blood bag under a horizontal swing at a predetermined flow rate. By the same measurement operation as described above, the dynamic light transmission intensity is obtained, and the rate of change of the light transmission intensity, which is the ratio of the phase difference value between the dynamic light transmission intensity and the static light transmission intensity, to the static light transmission intensity is calculated. An attempt is made to calculate the platelet ratio from a correlation coefficient between the calculated value and% DISK. By doing as described above, in the present invention, not only a highly reliable measurement result can be realized by measuring the transmitted light with a simple device, but also the blood bag in the horizontal state can be swung sideways. It is an object of the present invention to make it possible to carry out the measurement in a state where the storage conditions of the blood bag can be satisfied, and to be able to use the storage shaking device as it is.

[0010] Next, when the optical activity state measuring method according to the second aspect is used, in adjusting the platelet preparation layer for measurement to a predetermined thickness in the first aspect, the blood bag is first pressed by a flat plate. By forming a pressurized occluded part, after the thickness of the platelet preparation layer for measurement has become zero, thereby adjusting the thickness to a preset thickness by moving a flat plate, etc. Regardless of the size of the sheet thickness of the blood bag, the thickness of the platelet preparation layer for measurement is always kept the same to improve the reliability of the measurement result.

Further, in the measuring method according to the third aspect of the present invention, in measuring the dynamic light transmission intensity, the blood bag is set in advance to set the flow rate of the platelet preparation layer for measurement to a specific set value. The plane size of the blood and the volume of the blood are measured, and the swing cycle for laterally swinging the blood bag is adjusted based on this, so that the blood bag of any size can have a predetermined thickness. The platelet preparation for measurement in the same active state is made the same, and the platelet preparation in the same active state is measured so that the rate of change in light transmission intensity is not changed every time measurement is performed. We are trying to ensure high reliability of measurement results.

According to a fourth aspect of the present invention, the rays used in the first to third aspects are transmitted by irradiating infrared rays instead of adopting red light as in the past. It enables highly sensitive measurement for light.

According to a fifth aspect of the present invention, in the first to third aspects, when the blood bag is laterally swung in the lateral state, the reciprocating horizontal shaking or the reciprocating rotary shaking is performed. In this way, it is intended that the measurement can be performed while maintaining the most desirable conditions of the blood bag, and that the shaking device currently used is made available for the storage.

According to a sixth aspect of the present invention, the static light transmission intensity and the dynamic light transmission intensity measured according to the first to third aspects are directly adopted as measured values by one measurement. Rather, by calculating the average value of each measurement value in a plurality of times within the resting time and the oscillating time of the blood bag, the reliability of the rate of change in light transmission intensity is further improved. .

Next, a seventh aspect of the present invention relates to a measuring apparatus which can be used to carry out the above method. In the seventh aspect, a measuring section, an electric control analyzing section, and a horizontal reciprocating shaking section. The measuring unit is configured to include a properly formed measuring table, a blood bag holding unit including a flat plate that can be raised and lowered by a flat plate driving unit, and a light transmission intensity measuring unit. The electric control analysis unit performs dynamic light transmission by oscillating the measurement unit in the horizontal direction by operating the static light transmission intensity and the horizontal reciprocating shaking unit based on the input from the light transmission intensity measurement unit. A control unit for measuring the intensity,
A current analysis circuit unit capable of calculating the rate of change of the light transmission intensity based on the measurement result and finally calculating% DISK, whereby the optical device based on the transmitted light measurement according to claim 1 is constructed. Active state measurement method,
It is trying to be able to carry out with high precision by a simple structure.

According to an eighth aspect of the present invention, in addition to the configuration of the seventh aspect, a pressure sensor is provided so that the formation of the pressing closing portion can be confirmed by pressing the blood bag by the flat plate driving portion. After the confirmation, a platelet preparation layer for measurement having a predetermined thickness is formed by upward movement of the flat plate driving section, and even if the thickness of the blood bag is large or small, the platelet preparation layer for measurement is accurately formed to the predetermined thickness. Therefore, it is intended that the highly accurate measuring method according to claim 2 can be implemented.

According to the ninth aspect of the present invention, a weight measuring section is added to the configuration of the seventh aspect, and a large number of optical sensors are additionally provided in the measuring section. Can be measured, and the weight of the platelet preparation in the blood bag can be measured by the above-mentioned weight measuring unit, whereby the above-mentioned plane dimensions and the volume of the platelet preparation obtained from the weight can be obtained. It is possible to know what swing cycle is set so that the platelet preparation in the measurement platelet preparation layer can be swung at a predetermined flow rate, and thus the measurement method according to claim 3 has high reliability. It can be implemented with

Next, in the case of claim 10, the above-mentioned claim 7
9. In any one of Items 9 to 9, by providing an infrared light emitting diode and a photodiode in the light emitting unit and the light receiving unit provided in appropriate places, respectively, to reduce static light transmission intensity and dynamic light transmission intensity by infrared rays. It is possible to measure with high sensitivity and try to improve the reliability of the measurement result.

In the measuring device according to claim 11, the measuring device according to claim 7 is an optically active state unit measuring device,
By allowing the required number of adjacent measuring devices to swing sideways by the horizontal reciprocating shaking unit, the measurement of the optically active state of many blood bags can be processed at once. I have.

[0020]

According to the present invention, in order to achieve the above object, according to the present invention, a blood bag enclosing a platelet preparation, which is placed horizontally on a measuring table, is connected to the measuring table by a flat plate. By sandwiching the platelet between the two, the platelet preparation of the blood bag is divided into two reservoirs in communication with each other via the measurement platelet preparation layer set to a predetermined thickness, and the blood bag is in a stationary state. The platelet formulation layer for measurement is irradiated with a light beam in a direction perpendicular thereto, and the static light transmission intensity, which is an electrical output obtained by irradiating the transmitted light to a light receiving element, is measured. It is obtained by irradiating light in a direction orthogonal to this under a flowing state at a predetermined flow rate of the platelet preparation layer for measurement based on the lateral swing of the blood bag, and transmitting the transmitted light to the light receiving element. Dynamic light transmission intensity Then, the change rate of the light transmission intensity, that is, dynamic light transmission intensity−static light transmission intensity / static light transmission intensity, is calculated, and the change ratio and% DISK indicating the normal platelet ratio in the blood bag are calculated. From the correlation coefficient of
It is intended to provide a method for measuring the optically active state of a platelet preparation, wherein K is calculated.

In the second aspect, when the predetermined thickness of the platelet preparation layer for measurement according to the first aspect is set, the blood bag is sandwiched between the flat plate and the measuring table. After the platelet preparation of the blood bag is separated into two reservoirs by the pressing and closing part, the pressing and closing part is opened by the separating operation between the flat plate and the measuring table to measure a predetermined thickness. The difference is that the platelet product layer is formed so that the platelet product of the blood bag is divided into two reservoirs in a communicating state via the platelet product layer for measurement.

Furthermore, in order to set the flow rate of the platelet preparation layer for measurement to a predetermined flow rate according to the third aspect, the plane dimensions of the blood bag and the platelet preparation stored in the blood bag are set. Based on each measurement with the volume of
A step of adjusting the oscillation cycle of the lateral oscillation so that the blood plate bag for measurement flows at a predetermined flow rate by the lateral oscillation of the blood bag is added.

According to the first to third aspects, in the fourth aspect, the light beam irradiated in a direction perpendicular to the platelet preparation layer for measurement is an infrared ray. Is performed by reciprocating horizontal shaking or reciprocating rotary shaking, and in the case of claim 6, the static light transmission intensity and the dynamic light transmission intensity are measured within a predetermined rest time of the blood bag, The content is that the light transmission intensity change rate is calculated based on the average value of each measurement value, which is performed a plurality of times within the predetermined swing time.

According to a seventh aspect of the present invention, there is provided the measuring device according to the first aspect.
It can be used to carry out the measurement method described above, and consists of a measurement unit, an electrical control analysis unit capable of inputting required contents, and a horizontal reciprocating shaking unit. The measurement unit mounts a blood bag containing a platelet product horizontally. With a measuring table and a flat plate that can be moved up and down by a flat plate drive unit controlled by an electrical control analysis unit.
By sandwiching the blood bag between the measurement table and the platelet preparation of the blood bag through the measurement platelet preparation layer set to a predetermined thickness, the blood bag is communicated with the two reservoirs so that the blood can be separated. A light transmitting intensity measuring unit including a bag sandwiching unit, a light emitting unit that irradiates a light beam in a direction perpendicular to the measuring platelet preparation layer from the light emitting element, and a light receiving unit that transmits the light to the light receiving element. The electrical control analysis unit receives the electrical output obtained by irradiating the transmitted light to the light receiving element in the stationary state of the blood bag, measures the static light transmission intensity, and reciprocates in the horizontal direction. By oscillating the measuring unit laterally in the direction orthogonal to the flat plate by operating the shaking unit,
Under a flow state at a predetermined flow rate of the platelet formulation layer for measurement, a control unit that measures the dynamic light transmission intensity by receiving the electrical output obtained by making the transmitted light incident on the light receiving element,
From the measurement results, dynamic light transmission intensity−static light transmission intensity /
The change rate of the light transmission intensity, which is the static light transmission intensity, is calculated, and the change rate and% D indicating the normal platelet ratio in the blood bag.
It is an object of the present invention to provide an apparatus for measuring the optically active state of a platelet preparation, comprising: a current analysis circuit for calculating the% DISK from a correlation coefficient with ISK.

An eighth aspect of the present invention can be used to carry out the measuring method of the second aspect, wherein the blood bag holding section as one member constituting the measuring section of the seventh aspect measures the blood bag. A pressure sensor for detecting that the sandwiching pressure forms a pressing blockage in the blood bag by being sandwiched between the table and the blood bag, thereby separating the platelet preparation of the blood bag into two reservoirs. By having the plate moving upward by the plate driving section, the pressing closing portion is opened, and a platelet preparation layer for measurement having a predetermined thickness is formed. Thus, the platelet preparation of the blood bag is configured to be able to be separated in a state of communicating with the two reservoirs.

A ninth aspect of the present invention relates to a measuring apparatus capable of implementing the measuring method of the third aspect, wherein a weight measuring section is added to the configuration of the seventh aspect, and the measuring section includes a measuring section. With a large number of optical sensors closed by the blood bag on the table, an area measuring unit for measuring the plane size of the blood bag is provided, whereby the plane dimension value input from the area measuring unit and The horizontal reciprocating shaking unit is operated by the oscillation cycle calculated by the volume value calculated from the weight value and the density input from the weight measuring unit that measures the weight of the platelet product of the blood bag, and the measurement is performed. The configuration is such that a predetermined flow rate of the platelet preparation layer is obtained.

According to a tenth aspect, in the seventh to ninth aspects, the light emitting unit of the light transmission intensity measuring unit is provided in the measuring table, the light receiving unit is provided on a flat plate, and the light emitting element of the light emitting unit emits infrared light. The content of the diode is that a visible light cut type photodiode having an absorption peak near the wavelength of the infrared ray is adopted as a light receiving element of the light receiving unit.

According to an eleventh aspect of the present invention, the apparatus according to the seventh aspect is used as an optically active state unit measuring device, and a required number of such optically active state unit measuring devices are provided. However, it is characterized in that the horizontal reciprocating shaking unit is configured to be able to swing laterally.

[0029]

BRIEF DESCRIPTION OF THE DRAWINGS FIG.
Before explaining the method for measuring the optically active state of FIG.
The optical active state measuring device according to claims 7 and 8 will be described in detail below. The device is an input device 2a capable of sequentially introducing a measuring unit 1 and a required content input from a higher order. And an electric control analysis unit 2 displaying a numerical value required to be described later and a display device 2b for displaying a calculation result, and a lateral reciprocating swinging laterally in a direction of an arrow Y which is a front and rear direction. It is configured by stacking and mounting with the shaking unit 3.

The measuring section 1 is composed of a translucent blood bag B made of polyolefin or the like in which a platelet preparation A is enclosed.
The flat plate 1b can be moved up and down by a measuring table 1A capable of being placed horizontally and a flat plate driving unit 1a controlled by the electric control analysis unit 2, whereby the center of the blood bag B is moved by the flat plate 1b. And a blood bag holding portion 1B which can be held between the measuring table 1A in the direction of arrow X which is the left and right direction, and furthermore, the flat plate 1b and the blood as shown in FIG. The light transmission intensity measuring unit 1C includes a light emitting unit 1c provided vertically with respect to the bag B, and a light receiving unit 1d into which light transmitted therefrom and transmitted through the blood bag B is incident. ing.

Here, in the illustrated example, the measuring table 1A has a mounting table 1e, a cavity 1f, and a foot 1g mounted on the electrical control analysis unit 2, and the blood bag holding unit 1B
In the illustrated example, the flat plate driving units 1a provided on the left and right of the measuring table 1A are provided with supports 1h standing on the mounting top plate 1e.
And a plate-like gear 1i provided at one end thereof, and a flat plate 1b.
And a pair of motors 1k in which a gear head 1j is meshed with the plate gear 1i. The four corners of the flat plate 1b are positioned by four elevating columns 1m erected on the measuring table 1A and held so as to be able to move up and down. Therefore, the gear head 1j and the plate-shaped gear which are rotated by the operation of the motor 1k. 1i, the flat plate 1b
Can be moved up and down by a desired height.

Next, the embodiment shown as the light transmission intensity measuring section 1C will be described. In the embodiment shown in FIG. 1 and particularly in FIG. 1B, the light emitting section 1c is connected to the measuring table 1A.
The light beam radiated upward from here passes through the blood bag B through the transmission window 1n of the mounting table 1e in the measurement table 1A as described later in detail, and the transmitted light Are incident on the light receiving portion 1d mounted on the flat plate 1b, 1p in the figure indicates a light receiving element of the light receiving portion 1d, and 1q indicates that light rays from the outside are not incident on the light receiving element 1p. Shows a light-shielding plate in the light receiving section 1d provided on the flat plate 1b, and 1r denotes a light emitting element of the light emitting section 1c.
The light-emitting portion 1c may be located at any position, but in the illustrated example, the light-emitting portion 1c is provided at the center of the blood bag B in the left-right direction indicated by the arrow Y.

Further, as described in detail later, the electric control analysis unit 2 includes a control unit 2c for measuring static light transmission intensity and dynamic light transmission intensity, and a change rate of light transmission intensity obtained from the measurement result. And a current analysis circuit unit 2d for calculating% DISK. The electrical control analysis unit 2 is provided on the swinging top plate 3a of the horizontal reciprocating shaking unit 3 which is swung by this. Since it is placed via the footrest 2e,
The measuring table 1A reciprocates in the arrow Y direction at a predetermined swing cycle due to the swing.

As the horizontal reciprocating shaking unit 3 described above, a known reciprocating horizontal shaking device or a reciprocating rotary shaking device can be used, and as described above, the reciprocating shaking in the direction of arrow Y orthogonal to the flat plate 1b. In the figure, reference numeral 3b denotes a swing cycle adjusting knob, and 3c denotes a cycle indicator window for displaying the swing cycle. 1s clearly shown in FIG. 1 (B) corresponds to the flat plate 1 in the light transmission intensity measuring unit 1C.
b shows a pressure sensor provided on the lower surface, and by adding the pressure sensor 1 s in this way, the measuring device according to claim 8 is constituted, and this makes the measuring method of claim 2 easier. It can be implemented with high precision. In the figure, 3d indicates a shaking section main body, and 3e indicates a footrest thereof.

Therefore, the case where the measuring method of claim 1 is carried out using the measuring apparatus shown in FIG.
First, the blood bag B described above is mounted horizontally long on the mounting table 1e of the measuring table 1A in the direction of arrow Y as shown in the figure, and the flat plate driving section 1a of the blood bag holding section 1B is connected to the electrical control analysis section 2B. By moving the flat plate 1b down by the control unit 2c, the central portion of the blood bag B in the direction of the arrow Y is sandwiched between the flat plate 1b and the measuring table 1A to thereby obtain a predetermined length d.
Through the measuring platelet layer Ad set to, platelet formulation A of the blood bag B, and classified so that the communicating state to the two reservoir A ₁ and A _2.

Here, in practice, the platelet preparation layer for measurement Ad
The predetermined length d is set to about 3 mm, and then the light transmission intensity measuring unit 1C and the control unit 2c are operated, and the light emitting unit 1c irradiates a light beam in the orthogonal direction to the platelet preparation layer for measurement Ad. This allows the light beam to be transmitted to the flat plate 1.
b, the light is transmitted through the sheet of the blood bag B and the platelet preparation layer for measurement Ad through the light transmitting window 1n, and the transmitted light is made incident on the light receiving portion 1d.

Here, as the light transmission intensity measuring unit 1C, red visible light having a wavelength of 600 to 700 nm can be used as a light beam from the light emitting unit 1c as in the past, but a longer wavelength of around 950 nm is used. When the near-infrared light is used, the difference between the static light transmission intensity is and the dynamic light transmission intensity id, which will be described in detail later, becomes larger, and the rate of change id of the light transmission intensity is
-Is / is is preferable because it can be detected with high sensitivity. Also 950
It is preferable to use an infrared ray having a wavelength of nm or more because the measurement can be performed with higher sensitivity. For this purpose, a near-infrared light emitting diode having a wavelength of 950 nm is adopted as the light emitting element 1r of the light emitting section 1c, and a visible light cut-off type photodiode which does not react to the light of the measurement chamber is used as the light receiving element 1p of the light receiving section 1d. For example, as shown in FIG.
It is good to be provided on the top. Further, in the embodiment shown in the figure, the light emitting section 1c and the light receiving section 1d are opposed to each other with the platelet preparation layer for measurement Ad interposed therebetween. Of course, the light emitting section 1c and the light receiving section 1d are separately provided at appropriate places. A light beam that is the irradiation light using a light transmission device such as an optical fiber,
The transmitted light may be guided.

In the measuring method according to the first aspect,
As the next step, the static light transmission intensity is and the dynamic light transmission intensity id are measured by an arbitrary procedure by operating the light transmission intensity measurement unit 1C by the electrical control analysis unit 2. In this case, when explaining from the measurement of the static light transmission intensity is, the blood bag B is reciprocated in the horizontal reciprocating shaking unit 3.
In the stationary state without the operation, the light beam orthogonal to the platelet preparation layer for measurement Ad is emitted from the light emitting unit 1d, and the transmitted light is incident on the light receiving element 1r of the light receiving unit 1d. As a result, an electrical output corresponding to the intensity of the transmitted light, for example, a short-circuit current is generated as the output from the light receiving element 1r,
This is input to the electrical control analysis unit 2. Here, in practice, when the blood bag B is not set on the measurement table 1A and the electrical control analysis unit 2 sets the short-circuit current at that time to 1
If the adjustment is made to be about 00 μA, the short-circuit current as the static light transmission intensity is becomes about 60 μA.

Next, when trying to measure the short circuit current or other electrical output as the dynamic light transmission intensity, the horizontal reciprocating shaking unit 3 and the input device 2a of the electrical control analysis unit 2 are also used. In this manner, the platelet preparation layer for measurement Ad is swung in the direction of arrow Y in FIG. 1, whereby the platelet preparation layer for measurement Ad has a predetermined flow rate. The swing cycle must be adjusted. This is because when the flow velocity changes every time the measurement is performed, the measured change rates of the light transmission intensity do not become the same. Then, similarly to the measurement of the static light transmission intensity is described above, the light from the light emitting unit 1c is irradiated orthogonally under such a swinging state, and the transmitted light is incident on the light receiving unit 1d, thereby The obtained dynamic light transmission intensity id
The electrical output due to the short-circuit current or the like is also input to the electrical control analysis unit 2.

As described above, the static light transmission intensity is and the dynamic light transmission intensity id are measured.
When the oscillation cycle is 40 rpm, id is 3 more than is.
Measurement results can be obtained that increase by about 5 μA. Here, the principle of the measurement will be explained with reference to FIG. 2. In FIG. 2, the platelet 1b is measured so as to cross the center of the blood bag B so that the platelet preparation layer for measurement Ad has a predetermined thickness of several mm. The plate 1A and a pair of glass plates 1t, 1t 'hold the platelet preparation layer A for measurement.
d emits near-infrared light from the light emitting diode. At this time, if the blood bag B is left still, FIG.
As (A), since the platelet p has become an irregular sequence, whereas the transmitted light L ₁ to light L ₀ is reduced become soluble, when brought into sideways reciprocating blood bag B is a blood bag flow occurs strokes parallel to the direction of arrow Y in B, platelet p is Accordingly, to try to sequence as one direction shown in FIG. 2 (B), the transmitted light L ₁ of the relative light beam L ₀ it become large it becomes transmitted light L ₂ than.

As described above, the short-circuit current or the like as an electrical output from the phototransistor or the like is input to the electrical control analysis unit 2 as described above, so that the static light transmission intensity is and the dynamic light transmission The intensity id is measured, and the current analysis circuit unit 2d calculates the change rate of the light transmission intensity, that is, dynamic light transmission intensity id−static light transmission intensity is / static light transmission intensity is. Further, the% DISK is calculated from the correlation coefficient between the rate of change and% DISK indicating the normal platelet ratio of the platelet preparation A in the blood bag B. At this time, the interphase coefficient is It must be introduced in advance from the input device 2a of the electrical control analysis unit 2.

Further, in order to obtain the above-mentioned% DISK, it is practically desirable to carry out the following steps. This is illustrated in FIGS. 3 and 4, and as shown in FIG. Under the condition, the predetermined time T ₁
During (10 to 30 seconds), the static light transmission intensity is as a short-circuit current by the light receiving element 1p is sampled about 50 to 300 times, and the average value is calculated by the current analysis circuit unit 2d to obtain an average short-circuit. The static average light transmission intensity ism as a current is measured. Next, even under the above-mentioned swinging state of the blood bag, the short-circuit current is measured for the time T ₂ (10
Approximately 50 to 300 times during a period of about 30 sec), the average value thereof is calculated, and the dynamic light transmission intensity idm as an average short-circuit current is measured. / Ism, and calculates the% DIS from the correlation coefficient and the measured rate of change.
K may be calculated, and the result may be calculated by using the above-described display device 2b.
Will be displayed.

As shown in FIG. 4, if the thickness d of the platelet preparation layer for measurement Ad and the flow rate are constant as shown in FIG. 4, the rate of change of the light transmission intensity is% DISK. A linear relationship is established. Therefore, if the correlation coefficient known from this is input by the input device 2a of the electrical control analysis unit 2, the% DISK can be displayed on the display device 2b. Blood bag B packed with platelets
Then,% DISK is about 70%. Note that the above measurement may be performed two or more times continuously, and the final% DISK may be calculated from the average value.

As described above, in the measuring device according to the eighth aspect, a pressure sensor 1s is added to the measuring device according to the seventh aspect, thereby implementing the measuring method according to the second aspect.
It is easier and more accurate. That is, in the method, as set forth in claim 1, when the thickness of the platelet preparation layer for measurement Ad in the blood bag B is set to the predetermined thickness d, this is performed by the following steps.

That is, as described above, the blood bag B is sandwiched between the flat plate 1b and the measuring table 1A to firstly form a pressing blockage portion, whereby the platelet product A of the blood bag B is separated into two. Therefore, at this stage, the platelet preparation layer for measurement Ad mentioned above is separated at this stage.
Has not yet been formed. At this time, in order to form the above-mentioned pressing blockage, it is known that the sheet of the blood bag B has been pressed by the manual lowering of the flat plate 1b, and the manual lowering may be stopped here. As described above, it is preferable that the pressure sensor 1s is provided on the lower surface of the flat plate 1b, and when the pressure sensor 1s detects a predetermined pressure value, the operation of the flat plate driving unit 1a by the electric control analysis unit 2 is stopped.

In the measuring method according to the second aspect, after the pressing and closing portion is formed, the separating operation between the flat plate 1b and the measuring table 1A is performed, whereby the pressing and closing portion is opened and the predetermined thickness is reduced. Thus, the platelet preparation layer Ad for measurement of the thickness d is formed, whereby the platelet preparation of the blood bag B is separated into the two reservoirs A ₁ and A ₂ in a communicating state for the first time. Therefore, by the above-mentioned operation, once the platelet 1b according to FIG. 1 is lowered, the platelet preparation immediately below the plate 1b is once dislodged, and therefore, after the thickness thereof becomes zero, the platelet is set at the set height. 1b rises,
Regardless of the size of the sheet thickness of the blood bag B, the predetermined thickness d of the platelet preparation layer for measurement Ad becomes constant, and the light transmission intensity based on the static light transmission intensity and the dynamic light transmission intensity is reduced. The rate of change is made constant, and the reliability of the measurement result can be improved.

Next, a measuring device according to claim 9 will be described with reference to FIG.
This is different from the above-mentioned claim 7 in that the measuring unit 1
A is that a large number of optical sensors 1μ are provided in A, and a weight measuring unit 1D for measuring the weight of the platelet preparation A in the blood bag B is added. The optical sensor 1μ is provided on the mounting top plate 1e of the measuring table 1A so that the optical sensor 1μ can be closed by the blood bag B mounted on the measuring table 1A.
An area measuring unit 1v is configured so that an electric signal proportional to the plane size of the blood bag B can be input to the electric control analysis unit 2 based on the number of μ.

In the case of the embodiment shown in FIG. 5, the weight measuring section 1D is interposed between the measuring table 1A and the electric control analysis section 2 and is stacked on the measuring table 1A. The weight of the platelet preparation A in the blood bag B thus obtained is measured so that the electric signal is input to the electrical control analysis unit 2. Calculates the volume. Further, the horizontal reciprocating shaking unit 3 is reciprocated at a predetermined swing cycle as described above, whereby the platelet preparation layer for measurement Ad
Must always flow at a predetermined flow rate, but since the flow rate depends on the plane size of the blood bag B and the volume of the platelet preparation A, the flow rate is determined by the plane size and the volume measured as described above. Horizontal reciprocating shaker 3 to be determined
The platelet preparation layer for measurement A
d can always flow at a predetermined flow rate.

By using the measuring device according to the ninth aspect, the measuring method according to the third aspect becomes easy to carry out.
Based on the measured value of the volume of the platelet product stored in the blood bag, the horizontal swing of the blood bag B causes the platelet product layer for measurement Ad to flow at a predetermined flow rate. There is an additional difference in adjusting. At this time, it is desirable that the above-mentioned plane size and volume can be automatically measured by the area measuring unit 1v and the weight measuring unit 1D as shown in FIG. It may be detected by a simple operation. Further, the electrical control analysis unit 2 can include a management number reading device, a printing device, a storage device, and the like for recording and storing the management number and measurement result of the blood to be measured. In the same figure, 1w indicates a footrest of the weight measuring unit 1D, and 1x indicates a weight display window.

Next, the measuring device according to claim 11 will be described with reference to FIG. 6. Here, the device according to claim 7 is referred to as an optically active unit measuring device 1E, and these are placed in a predetermined measuring chamber 1F. As the horizontal reciprocating shaker 3, a horizontal horizontal shaker or a reciprocating rotary shaker can be adopted as in the above-described apparatus, and the horizontal reciprocating shaker 3 is used for the horizontal reciprocal shaker. The machine 3f is operated only by a pair, and the swinging top 3a swings by the operation.
Are provided in a desired number of stages above and below, and by each of these swinging top plates 3a,
Each of the plurality of optically active state unit measuring devices 1E is made to oscillate sideways at the same time, so that the% DISK can be measured at once for a large number of blood bags B. Therefore, in order to configure the measurement device, a reciprocating shaker used for storing the platelet preparation A on a daily basis may be adopted as it is, and other components may be attached. It is possible to measure the% DISK continuously or intermittently and store the result while preserving by horizontal shaking.

[0051]

Since the present invention can be implemented or configured as described above, according to the measuring method of the first aspect, the platelet preparation can be of a predetermined thickness without depending on the conventional fluctuation amplitude of the scattered light by platelets. % DISK can be measured from the static light transmission intensity by the transmitted light and the dynamic light transmission intensity under the condition that the disk is oscillated at a predetermined flow rate, so that a high reliability can be obtained with a simple device. Can be provided, and the measurement purpose can be achieved by the lateral swinging of the blood bag. Therefore, the measurement can be performed in the stored state of the blood bag, and the storage shaker can be used as it is.

Further, according to the measuring method of the present invention, when the thickness of the platelet preparation layer for measurement is set to a predetermined thickness, it is not restricted to a predetermined thickness from the beginning, but is once set to a zero-thickness state by a flat plate, and this is set as a reference. Since the thickness of the blood bag is varied until the predetermined thickness is obtained, the predetermined thickness can always be set with high reliability even if the thickness of the blood bag has various dimensions, whereby the correct% DISK measurement can be performed. Value can be provided.

Next, in the measuring method according to the third aspect, the plane size of the blood bag and the weight of the platelet preparation sealed therein are known, and the swing cycle of the blood bag by the horizontal reciprocating shaking unit is determined based on the both. Since the calculated platelet preparation layer is made to always flow at a predetermined flow rate, the reliability of the measurement result of% DISK can be further improved.

According to the fourth aspect, the difference between the dynamic light transmission intensity and the static light transmission intensity can be increased by using infrared rays as the light beams according to the first to third aspects, and the measurement accuracy can be increased by improving the sensitivity. According to the fifth aspect, a reciprocating horizontal shaking device or a reciprocating rotary shaking device is used in the transverse reciprocating shaking portion, so that the device used for storage can be utilized as described above. In the case of 6, the measurement of the average static light transmission intensity and the dynamic light transmission intensity can be performed quickly, and high reliability can be given to the rate of change of the light transmission intensity.

According to the seventh aspect of the present invention, the measuring apparatus comprises a measuring section, an electrical control analyzing section, and a horizontal reciprocating shaking section. The measuring section includes a measuring table, a flat plate driving section and a blood bag holding section having a flat plate, A light transmission intensity measuring unit, the electrical control analyzing unit has a control unit and a current analyzing circuit unit, and the horizontal reciprocating shaking unit swings the blood bag placed on the measuring table in a horizontal state. Since the measuring method is configured so as to be free, the measuring method according to claim 1 can be performed with high accuracy.

According to the measuring apparatus of claim 8, since the pressure sensor is provided on the flat plate of claim 7, the platelet preparation layer for measurement in the blood bag is provided in the measuring method of claim 2. When the predetermined thickness is set, it is possible to accurately detect the state of formation of the pressing blockage due to the abutment of the sheet of the blood bag, and to maintain the predetermined thickness constant with high accuracy. Regarding the measurement result, high reliability can be always maintained without being affected by the sheet thickness of the blood bag.

According to the ninth aspect of the present invention, since the weight measuring section is added to that of the seventh aspect, and the measuring section is additionally provided with an area measuring section using an optical sensor, the dimensions of the blood bag and the platelet preparation are determined in advance. Since the weight can be automatically measured and its volume can also be measured, the oscillating cycle of the horizontal reciprocating shaking unit is determined using the measurement result when the measuring method of claim 3 is performed. Thus, the platelet preparation layer for measurement can be always swung at a predetermined flow rate with high accuracy, and the reliability of the measurement of% DISK can be improved.

According to a tenth aspect of the present invention, there is provided a measuring apparatus as set forth in the seventh aspect.
In paragraphs 9 to 9, infrared light emitting diode photodiodes are provided in the light emitting part and the light receiving part in the light transmission intensity measuring part, so that the static light transmission intensity and dynamic light transmission intensity due to infrared light can be measured with high sensitivity. The accuracy of the measurement result can be improved.

According to the eleventh aspect of the present invention, a plurality of optically active state unit measuring apparatuses can be operated in common for storage and measurement of a blood bag by one horizontal reciprocating shaking unit. Therefore, efficient measurement of% DISK
It can be implemented with inexpensive equipment.

[Brief description of the drawings]

FIG. 1 shows an apparatus for measuring the optically active state of a platelet preparation according to claims 8 and 9 of the present invention, wherein (A) is a perspective view thereof,
(B) is a schematic right side view showing a partial longitudinal section of (A).

FIG. 2 shows a light transmission state when a blood bag is pressed by a flat plate until a platelet preparation layer for measurement having a predetermined thickness is formed, and a light beam is applied to the blood bag. (B) is a vertical front view of the preparation in a stationary state, and FIG.

FIG. 3 shows a measured value of a short-circuit current with respect to the time of a light-receiving element that has received the transmitted light in a stationary state of the platelet preparation layer for measurement, and a short-circuited time with respect to the time of a light-receiving element that has received the transmitted light under the rocking state. 5 is a chart showing measured values of current.

FIG. 4 is a chart showing the relationship between% DISK of normal platelet preparations and the rate of change in light transmission intensity.

FIG. 5 is a perspective view showing a previous optically active state measuring device according to claim 9;

FIG. 6 is a perspective view showing an optically active state measuring device according to the eleventh embodiment.

[Explanation of symbols]

DESCRIPTION OF SYMBOLS 1 Measuring part 1A Measuring table 1B Blood bag holding part 1C Light transmission intensity measuring part 1D Weight measuring part 1E Optical active state unit measuring device 1a Flat plate driving part 1b Flat plate 1c Light emitting part 1d Light receiving part 1p Light receiving element 1r Light emitting element 1s Pressure sensor 1μ Optical sensor 1v Area measurement unit 2 Electrical control analysis unit 2c Control unit 2d Current analysis circuit unit 3 Horizontal reciprocating shaking unit A Platelet preparation A ₁ storage unit A ₂ storage unit Ad Platelet preparation layer for measurement L ₀ ray L ₁ transmitted light L ₂ transmitted light d predetermined thickness is static light transmission intensity id dynamic light transmission intensity

────────────────────────────────────────────────── ─── Continuation of front page (72) Inventor Kenchi Kahara 1007 Izumizawa, Chitose-shi, Hokkaido Daido Hokusan Co., Ltd. Chitose Research Institute (56) References JP-A-5-240863 (JP, A) JP-A-60 -113153 (JP, A) (58) Fields investigated (Int. Cl. ⁶ , DB name) G01N 33/48-33/98 G01N 15/00

Claims (11)

(57) [Claims] 1. A platelet-containing blood bag, which is placed horizontally on a measurement table, is sandwiched between the measurement table and a plate by a flat plate. The platelet product in the blood bag is divided into two reservoirs in communication with each other, and the platelet product layer for measurement is irradiated with a light beam in a direction perpendicular to the blood plate under the stationary state of the blood bag, and the transmitted light Is measured by measuring the static light transmission intensity, which is the electrical output obtained by inputting the light into the light-receiving element, and the platelet preparation layer for measurement under a flowing state at a predetermined flow rate based on the lateral swing of the blood bag. Then, a light in a direction orthogonal to this is irradiated, and the transmitted light is incident on the light receiving element to measure a dynamic light transmission intensity, which is an electrical output obtained. Typical light transmission intensity /
The change rate of the light transmission intensity, which is the static light transmission intensity, is calculated, and the change rate and% D indicating a normal platelet ratio in the blood bag.
A method for measuring the optically active state of a platelet preparation, wherein the% DISK is calculated from a correlation coefficient with ISK. 2. A platelet product-enclosed blood bag, which is placed laterally on a measurement table, is sandwiched between the measurement table and a plate by a flat plate. Is separated into two reservoirs, and then the pressing and closing part is opened by separating the flat plate and the measuring table to form a platelet preparation layer for measurement having a predetermined thickness, whereby the measurement is performed. Via the platelet product layer, the platelet product of the blood bag is divided into two reservoirs in communication with each other, and a light beam in a direction perpendicular to the platelet product layer for measurement is provided while the blood bag is stationary. Irradiate, measure the static light transmission intensity, which is the electrical output obtained by making the transmitted light incident on the light receiving element, and determine the predetermined platelet formulation layer for measurement based on the lateral swing of the blood bag. Under flow conditions due to flow velocity , Irradiate light in the direction orthogonal to this,
A dynamic light transmission intensity, which is an electrical output obtained by making the transmitted light incident on the light receiving element, is measured, and then the light transmission is calculated as dynamic light transmission intensity−static light transmission intensity / static light transmission intensity. The change rate of the intensity is calculated, and from the correlation coefficient between the change rate and% DISK indicating the normal platelet ratio in the blood bag,
A method for measuring the optically active state of a platelet preparation, wherein the% DISK is calculated. 3. A platelet-containing blood bag, which is placed horizontally on a measurement table, is sandwiched between the measurement table with a flat plate, and the platelet-containing blood layer for measurement is set to a predetermined thickness. The platelet product in the blood bag is divided into two reservoirs in communication with each other, and the platelet product layer for measurement is irradiated with a light beam in a direction perpendicular to the blood plate under the stationary state of the blood bag, and the transmitted light Is measured by measuring the static light transmission intensity, which is the electrical output obtained by entering the light-receiving element, based on the measured values of the planar dimensions of the blood bag and the volume of the platelet product stored in the blood bag. By the lateral swing of the blood bag, the swing cycle of the transverse swing is adjusted such that the platelet preparation layer for measurement flows at a predetermined flow rate,
Under the flowing state, irradiating light in a direction perpendicular to the flowing direction, and measuring the dynamic light transmission intensity, which is an electrical output obtained by making the transmitted light incident on the light receiving element, The change rate of light transmission intensity, which is light transmission intensity-static light transmission intensity / static light transmission intensity, is calculated, and from the correlation coefficient between the change rate and% DISK indicating a normal platelet ratio in the blood bag, A method for measuring the optically active state of a platelet preparation, wherein the% DISK is calculated. 4. The method for measuring the optically active state of a platelet preparation according to any one of claims 1 to 3, wherein the light beam irradiated in a direction perpendicular to the platelet preparation layer for measurement is infrared light. 5. The blood bag according to claim 1, wherein the lateral swing of the blood bag is performed by reciprocating horizontal shaking or reciprocating rotary shaking.
4. The method for measuring the optically active state of a platelet preparation according to any one of items 3 to 3. 6. The measurement of the static light transmission intensity and the dynamic light transmission intensity is performed a plurality of times within a predetermined stationary time of the blood bag and within a predetermined swinging time, respectively, and an average value of the respective measured values is measured. And calculating the rate of change of the light transmission intensity.
4. The method for measuring the optically active state of a platelet preparation according to any one of items 3 to 3. 7. A measuring unit, an electric control analyzing unit capable of inputting required contents and a horizontal reciprocating shaking unit, wherein the measuring unit includes a measuring table on which a blood bag enclosing a platelet preparation is horizontally mounted,
By a flat plate that can be moved up and down by a flat plate drive unit controlled by an electrical control analysis unit, by sandwiching the blood bag between the measurement table and the platelet preparation layer for measurement set to a predetermined thickness, The blood bag sandwiching portion, which is capable of separating the platelet preparation of the blood bag in communication with the two storage sections and is freely separated, and the light emitting section that irradiates a light beam in a direction perpendicular to the blood plate holding layer to the measurement platelet preparation layer, A light transmission intensity measuring unit comprising a light receiving unit for transmitting the transmitted light to the light receiving element, and the electrical control analysis unit allows the transmitted light to enter the light receiving element while the blood bag is stationary. Measure the static light transmission intensity by receiving the obtained electrical output,
By oscillating the measurement unit horizontally in the direction perpendicular to the flat plate by the operation of the horizontal reciprocating shaking unit, the transmitted light is incident on the light receiving element under the flow state at a predetermined flow rate of the platelet preparation layer for measurement. A control unit that measures the dynamic light transmission intensity by receiving the obtained electrical output, and, based on the measurement result, a change rate of the light transmission intensity, which is (dynamic light transmission intensity−static light transmission intensity) / (static light transmission intensity) And a current analysis circuit unit for calculating the% DISK from a correlation coefficient between the rate of change and% DISK indicating a normal platelet ratio in the blood bag. Optical activity state measuring device. 8. A measuring section, an electric control analyzing section capable of inputting required contents and a horizontal reciprocating shaking section, wherein the measuring section includes a measuring table on which a blood bag enclosing a platelet preparation is horizontally mounted,
By sandwiching the blood bag between the measurement table and the flat plate that can be moved up and down by the flat plate drive unit controlled by the electrical control analysis unit, the clamping pressure forms a pressing blockage in the blood bag, This has a pressure sensor for detecting that the platelet agent of the blood bag has been separated into the two storage portions, and opens the pressing closing portion by moving the flat plate upward by the flat plate driving portion. Then, by forming a platelet preparation layer for measurement having a predetermined thickness, the blood bag holding section that can freely separate the platelet preparation of the blood bag through the measurement platelet preparation layer in communication with the two storage sections. A light-emitting unit that irradiates a light beam in a direction perpendicular to the platelet preparation layer from the light-emitting element to the measurement platelet preparation layer, and a light transmission intensity measurement unit that includes a light-receiving unit that transmits the light to the light-receiving element. And electricity Together with the control analysis unit measures the static light transmission intensity by receiving an electrical output obtained by entering the transmitted light of the at rest state of the blood back to the light receiving element,
By oscillating the measurement unit horizontally in the direction perpendicular to the flat plate by the operation of the horizontal reciprocating shaking unit, the transmitted light is incident on the light receiving element under the flow state at a predetermined flow rate of the platelet preparation layer for measurement. A control unit that measures the dynamic light transmission intensity by receiving the obtained electrical output, and, based on the measurement result, a change rate of the light transmission intensity, which is (dynamic light transmission intensity−static light transmission intensity) / (static light transmission intensity) And a current analysis circuit unit for calculating the% DISK from a correlation coefficient between the rate of change and% DISK indicating a normal platelet ratio in the blood bag. Optical activity state measuring device. 9. A measuring unit, an electric control analyzing unit capable of inputting required contents, a weight measuring unit, and a horizontal reciprocating shaking unit, wherein the measuring unit includes a measuring table on which a blood bag containing a platelet preparation is horizontally mounted. A flat plate that can be moved up and down by a flat plate drive unit controlled by an electrical control analysis unit, by sandwiching the blood bag between the plate and a measurement table, through a platelet preparation layer for measurement set to a predetermined thickness. The blood bag holding section, which is capable of separating the platelet preparation of the blood bag in communication with the two storage sections in a communicating state, and a large number of optical sensors closed by the blood bag on the measurement table, the planar size of the blood bag. An area measuring unit for measuring the light intensity, a light emitting unit for irradiating a light beam in a direction orthogonal to the platelet preparation layer from the light emitting element, and a light receiving unit for transmitting the transmitted light to the light receiving element. Light transmission intensity measurement section The electrical control analysis unit receives the electrical output obtained by making the transmitted light incident on the light receiving element in a stationary state of the blood bag, measures the static light transmission intensity, and measures the area. By the swing cycle calculated by the plane dimension value input from the measurement unit and the volume value calculated from the weight value and the density input from the weight measurement unit that measures the weight of the platelet product of the blood bag, the landscape orientation By operating the reciprocating shaking unit, under the flow state of the measurement platelet preparation layer at a predetermined flow rate, receiving the electrical output obtained by making the transmitted light incident on the light receiving element, the dynamic light transmission intensity The control unit to measure, and from the measurement result, calculate the change rate of the light transmission intensity, which is dynamic light transmission intensity−static light transmission intensity / static light transmission intensity, and calculate the change rate and the normal rate in the blood bag. % DISK indicating platelet ratio and From the correlation coefficient, optically active state measuring apparatus platelet product, wherein a current analyzing circuit is provided for calculating the% DISK. 10. A light emitting section of the light transmission intensity measuring section is provided inside the measuring table, and a light receiving section is provided on a flat plate. An infrared light emitting diode is provided as a light emitting element of the light emitting section, and a light receiving element of the light receiving section is provided. 10. A photoode having an absorption peak near the wavelength of the infrared ray is adopted.
Item 14. The optically active state measuring device for a platelet preparation according to Item 6. 11. An optical active state unit measuring device comprises a measuring section, an electric control analyzing section capable of inputting required contents, and a horizontal reciprocating shaking section, wherein the measuring section horizontally mounts a blood bag containing a platelet preparation. A measuring table to be placed, and a flat plate that can be moved up and down by a flat plate driving unit controlled by an electrical control analysis unit, and the blood bag is sandwiched between the measuring table and a measuring table set to a predetermined thickness. Through the platelet product layer, the platelet product of the blood bag is separated into two blood reservoirs in a communicating state and is freely separable, and the platelet product layer for measurement emits a light beam in a direction orthogonal to this. And a light transmission intensity measuring unit comprising a light receiving unit whose transmitted light is incident on a light receiving element, and an electrical control analysis unit converts the transmitted light in a stationary state of the blood bag. Light incident on light receiving element In addition to measuring the static light transmission intensity by receiving the electrical output obtained in this way, by operating the horizontal reciprocating shaking unit, the measuring unit is oscillated laterally in the direction perpendicular to the flat plate, so that the predetermined platelet preparation layer for measurement is determined. Under a flow state caused by the flow velocity, a control unit that measures the dynamic light transmission intensity by receiving an electric output obtained by making the transmitted light incident on the light receiving element, and obtains the dynamic light transmission intensity from the measurement result. Calculating the rate of change of the light transmission intensity (static light transmission intensity / static light transmission intensity), and calculating the% DISK from the correlation coefficient between the rate of change and% DISK indicating the normal platelet ratio in the blood bag. And a current analysis circuit unit for calculating the optical active state unit measuring device, and a required number of the optical active state unit measuring devices are provided adjacent to each other. Can swing sideways by Optically active state measuring apparatus for measuring platelet preparation characterized by being configured.