JP2952016B2 - Cosmetics excluding bath agent - Google Patents

Description

Description: FIELD OF THE INVENTION The present invention relates to cosmetics excluding bath agents, and particularly to cosmetics excluding bath agents that give the skin good moisturizing properties.

(Prior Art) Conventionally, in order to maintain healthy and beautiful skin, a humectant that gives a suitable amount of moisture to the skin has been incorporated into cosmetics. Glycerin, propylene glycol, 1,3-butylene glycol, pyrrolidone carboxylate and the like are used as such humectants. However, these may absorb the moisture of the stratum corneum of the skin and work to lose the moisture of the skin, and if they are contained in cosmetics in a large amount, they may give a sticky feeling. . Furthermore, glycerin and the like absorb water in the environment, but when they are transferred to a low-humidity environment, they readily release water, and have a shortcoming in the sustainability of moisture retention. To compensate for these drawbacks, sodium hyaluronate, a natural product extract, has recently been used, but it forms a film on the skin in low-humidity environments. There was a problem. For these reasons, aloe extract and yoquinin extract, which are extracts of natural products, have recently been used in the formulation of cosmetics.

On the other hand, spots and freckles are said to involve melanin biosynthesized by the action of tyrosinase using tyrosine as a starting material in vivo. For this reason, it is conceivable to add a substance that inhibits the activity of monophenol monooxygenase in cosmetics as one of the measures to keep skin whitening. These include crude drugs and extracts of mushrooms. Has been reported to have a whitening effect (JP-B-59-48808, JP-A-2-4971).
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However, the crude drug extract has a unique crude drug smell and has no tyrosinase activity inhibitory action, and thus cannot be said to be satisfactory. In addition, mushrooms having a large tyrosinase activity-inhibiting activity still have a problem in collection from nature.

(Problems to be Solved by the Invention) The present invention relates to Zizania latifolia.
The purpose of the present invention is to obtain cosmetics excluding bath agents which are excellent in water retention and have an inhibitory activity on tyrosinase activity by using an extract extracted from the fungi of tifolia (Japanese name is "Makomo").

(Means for Solving the Problems) The present invention is a cosmetic product excluding a bath agent containing an extract extracted from the fungus of Zizania latifolia.

Zizania latifolia is a plant that grows naturally in ponds and rivers and forms fungal gall at its stem base. What grows or grows in China or Taiwan is formed by enlargement of fungi, which is called "Kouhaku". The present invention uses an extract (hereinafter, this extract is referred to as “Kohaku extract”) extracted from such fungi as a cosmetic formulation. The amount of the amber extract used in the cosmetic composition of the present invention is not particularly limited, and an appropriate amount can be used.

First, the present inventors have proposed a method for extracting an agar extract from fungi of Zizania latifolia and a bath agent containing the extract (Japanese Patent Application Laid-Open No. 2-62827).
As the amber extract used in the present invention, those extracted by the method disclosed in the above proposal are used as they are.
As is clear from the above proposal, this amber extract has no irritation or phototoxicity in an acute skin irritation test or phototoxicity test. In addition, a mutagenicity test was not performed in a mutagenicity test on a concentrate obtained by distilling ethanol from the extract, and the safety of skin toxicity was confirmed. Furthermore, this extract has a fragrance having a slight bitterness and sweetness, such as a mixture of herbal fragrance and resinous fragrance, and is an extract having no problem in flavor when mixed with other natural fragrances. The inventor has further studied this amber extract, and this extract has a moderate hygroscopic property, and has a moderate moisture retaining property even under low humidity, and has a moisture absorbing property and excellent moisture retaining property. It was confirmed.

That is, cosmetics excluding the bath agent containing the amber extract were made, and these were compared with cosmetics containing aloe extract and yoquinin extract, which are conventionally known natural moisturizers. As shown, it was found that the skin moisturizer (moist feeling), the smoothness (smoothness) and the elasticity of the skin (skin texture) were superior to the control natural product humectant.

The inventor further noted that the amber extract used in the present invention is:
As shown in Experimental Example 2 below, it was found that the compound had an inhibitory effect on tyrosinase activity.

That is, the amber extract extracted by the method described in JP-A-2-49710 is concentrated under reduced pressure to distill off ethanol, and the ethanol is distilled off.
%, And the filtrate was used as a tyrosinase activity inhibition measurement sample. When this measurement sample was added to the reaction system for measuring tyrosinase activity, it was confirmed that the tyrosinase activity was significantly suppressed. Therefore, according to the present invention, it is possible to provide cosmetics excluding bath agents which can be expected to have a whitening effect by inhibiting tyrosinase activity.

 Hereinafter, the present invention will be further described with reference to experimental examples.

EXPERIMENTAL EXAMPLE 1 Test sample Taiwanese sea cucumber fungi were cut into small pieces and freeze-dried. 100 g of the fungus was steamed for 1 hour in a steamer. Then, 500 g of ethanol was added to the fungus extract and extraction was performed at room temperature for 7 days. This extract was filtered to obtain an ethanol extract. The ethanol extract was heated on a water bath to evaporate off the ethanol. Then, the sample was heated in a dryer at 110 ° C. for 4 hours to evaporate water, thereby obtaining a test sample.

In addition to the above, the following were processed for comparison as test samples.

Glycerin (special grade, manufactured by Wako Pure Chemical Industries, Ltd.) Aloe ethanol extract (extract of aloe powder (produced by Nippon Shokubai Co., Ltd.) extracted with ethanol) dl-pyrrolidone carboxylate 50% aqueous solution (Ajinomoto Co., Ltd.) )) Sodium hyaluronate (hyaluron extract Snowden Co., Ltd.) 1,3-butylene glycol (daicel chemical industry) aloe ethanol extract, dl-pyrrolidone carboxylate aqueous solution, sodium hyaluronate The treatment was carried out in the same manner as the ethanol extract to obtain a test paper amount.
The glycerin was immediately heated in a dryer at 110 ° C. for 4 hours to evaporate and remove the contained water to obtain a test sample.
For 1,3 butylene glycol, a test sample was left overnight in a desiccator containing deer gel.

Test method (1) Measurement of hygroscopicity An aqueous solution oversaturated with NH ₄ Cl was placed in a desiccator (with a relative humidity of 7).
(9.2%), the test sample was left for the time shown in Table 1, the weight increase rate was measured, and the hygroscopicity was examined. The results are shown in Table 1.

(1) Measurement of moisture retention The sample left in the desiccator for 48 hours in the above (1) Measurement of moisture absorption was then transferred to another desiccator containing silica gel, and left as it was for 24 hours and 48 hours, and the weight of the sample was measured. From now on, measure the water retention at this point,
The moisture retention was measured. The results are shown in Table 1.

As shown in Table 1, it can be seen that the amber extract has high hygroscopicity and good moisture retention. Note that dl−
Sodium pyrrolidone carboxylate and sodium hyaluronate have good hygroscopicity and moisture retention, but as described above, these are preferable because they form a film on the skin or absorb moisture in the stratum corneum of the skin. Absent. Aloe extract and 1,3-butylene glycol are not preferred because of their low moisture retention.

Experimental Example 2 The tyrosinase activity inhibitory effect was measured as follows.

(Preparation of Test Solution for Measurement) In the same manner as in Experimental Example 1, an amber ethanol extract was obtained. Ethanol was distilled off at 40 ° C. from 10 ml of this ethanol extract using a rotary evaporator. This one
25 ml of a 30% aqueous solution of 3-butylene glycol was added, and the mixture was refluxed on a boiling water bath for 30 minutes. Thereafter, the mixture was cooled and filtered to prepare a sample solution.

Similarly, ethanol extract 5ml and 2.5ml
Prepared a sample solution.

(Measurement of inhibitory action) 1 ml of 1-tyrosine aqueous solution (concentration, 0.3 mg / ml) was placed in a test tube.
And 1 ml of McIlvaine buffer (pH 6.8), 0.9 ml of a sample solution was added, and the mixture was incubated for 10 minutes in a thermostat at 37 ° C. to obtain a test solution.

In a separate test tube, add 1 ml each of the 1-tyrosine aqueous solution and the McIlvaine buffer solution, and use a 30% aqueous solution of 1,3-butylene glycol instead of the test solution as a blank test.
0.9 ml was added, and the mixture was incubated in a constant temperature water bath at 37 ° C. for 10 minutes to obtain a black solution.

In the test solution and the blank solution, a monophenol monooxygenase enzyme solution (concentration, 1) was used as a tyrosinase enzyme solution.
(0.1 mg / ml in McIlvaine buffer), and the mixture was stirred well and immediately set in a self-recording spectrophotometer.
The absorbance was measured with. The tyrosinase activity inhibition rate was determined by the following equation. Table 2 shows the results.

B ₀ ; Absorbance value of 0 minutes after the enzyme solution of the blank solution was introduced B ₁₀ ; Absorbance value of 10 minutes after the introduction of the enzyme solution of the blank solution A ₀ ; Absorbance value of 0 minutes after the introduction of the enzyme solution of the test solution A ₁₀ ; Test Absorbance value 10 minutes after the addition of the enzyme solution of the solution In addition, the measurement method as described above, from the aspect of the use of actually applying cosmetics to the skin except the bath agent containing amber extract, This is because the cochlear extract and tyrosine are present at the same time, and are stimulated to activate monophenol monooxygenase to react with tyrosine.

As a comparative example, 10 ml of aloe ethanol extract was distilled off at 40 ° C. using a rotary evaporator according to the description in the above section (Measurement of inhibitory action), and 25 ml of a 30% aqueous solution of 1,3-butylene glycol was added and refluxed. , Cooled and filtered to prepare a sample solution. The tyrosinase activity inhibition rate was measured using this sample. As a result, the suppression rate was 34.5%.

Koaku extract can be extracted with water and an organic solvent, and then this solvent can be distilled off and then added to cosmetics.However, it is described in cosmetic primordium such as ethanol and 1,3-butylene glycol. The solvent used need not be distilled off, and can be used as it is. All the amber extract in each of the following examples was dissolved in ethanol.

Example 1 (Astringent lotion) An astringent lotion was prepared by using the composition (A) shown in Table 3 as a base and blending each wetting agent as described in Table 4.

The skin lotion effect test was performed on this lotion.

Apply the above-mentioned astringent lotion to 20 female subjects (30-50 years old) who complain of rough skin, fine wrinkles, dry skin, etc., for 2-3 consecutive days by applying 2-3 drops of lotion to the inner part of the upper arm of the subject for 15 days. , Two times in the evening, and the effect was examined. As a result, with respect to the wettability, smoothness, and elasticity of the skin, those who answered that the skin felt moist, the skin became smooth, and the skin was stretched were as shown in Table 5; It is clear that the lotion containing the extract has excellent skin wettability, smoothness, and elasticity as compared with the lotion containing another wetting agent.

Example 2 (Cream) A cream having the composition shown in Table 6 was prepared.

Example 3 (Emulsion) An emulsion having the composition shown in Table 7 was prepared.

Example 4 (Shampoo) A shampoo having the composition shown in Table 8 was prepared.

Example 5 (Rinse) A rinse having the composition shown in Table 9 was prepared.

Example 6 (Pack) A pack having the composition shown in Table 10 was prepared.

──────────────────────────────────────────────────続 き Continuing on the front page (72) Inventor Shoichi Ogawa 3-31-3 Minamiurawa, Urawa-shi, Saitama (72) Inventor Kazuo Shinbayashi 4-29-13-103, Kitazawa, Setagaya-ku, Tokyo (56) Reference Document JP-A-2-62827 (JP, A) JP-A-55-164613 (JP, A) (58) Fields investigated (Int. Cl. ⁶ , DB name) A61K 7/00 A61K 7/075

Claims (1)

(57) [Claims] 1. A cosmetic excluding a bath agent containing an extract extracted from the fungus of Zizania latifolia.