JP3382149B2 - External preparation for head - Google Patents

Description

Description: BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an external preparation for the head such as a hair restorer having a synergistically improved hair growth effect such as a hair loss preventing effect and a hair growth effect. For more information, see Agaric Mushroom ( Agar
icus blazei Murill) One or more selected from mycelium extract and culture filtrate, and sage ( Salvia offic)
inalis L.), hops ( Humulus lupulus L.), rosemary ( Rosmarinus officinalis L.), hypericum ( Hypericum erectum Thunb.), mint ( Mentha arven )
sis L. var. piperascens Malinv.) , peppermint (Mentha piperita L.), chamomile (Matricaria chamo
milla L.), Arnicamontana L., Thymus vulgaris L., and one or more selected from extracts thereof as an active ingredient. is there. [0002] Alopecia is caused by poor nutritional intake, decreased scalp physiology due to excessive occurrence of dandruff, and decreased metabolic functions of hair follicles and hair bulbs caused by decreased blood flow in scalp subcutaneous tissues and peripheral blood vessels. It is caused by various causes such as deterioration and mental stress. In order to treat and improve such alopecia, estrogen such as estrone, estradiol, ethinyl estradiol, vitamin E and its derivatives, assembly extract, garlic extract, carrot extract, aloe extract, cepharanthin, chloride Peripheral vascular blood flow promoters such as carpronium and minoxidil, local stimulants such as pepper tincture, cantaris tincture, ginger tincture, peppermint oil, l-menthol, camphor, keratolytic agents such as resorcin, salicylic acid, lactic acid, pyridoxine and Antiseborrheic agents such as derivatives thereof, bactericides such as zinc pyrithione, benzalkonium chloride, benzethonium chloride, chlorhexidine, hinokitiol, pantothenic acid and derivatives thereof,
Placental extract, biotin, hair root activator such as pentadecanoic acid glyceride, glycyrrhizic acid and its derivatives, anti-inflammatory agents such as β-glycyrrhetinic acid, allantoin, azulene, ε-aminocaproic acid, hydrocortisone, vitamins A, B ₂ , B ₁₂ , D and the like, and amino acids such as cysteine, serine, methionine, leucine and tryptophan, and many other hair growth and hair growth components have been used. Further, mainly for the treatment and improvement of male pattern baldness, testosterone 5α- catalyzes the conversion of testosterone to active dihydrotestosterone.
Many that inhibit reductase activity have been screened. Known inhibitors of the enzyme include steroid substances such as progesterone and deoxycorticosterone, as well as extracts of various types of plants. Also, oxendrone, spironolactone, cyproterone acetate, and the like are said to inhibit dihydrotestosterone from binding to its receptor. [0005] However, some of the conventionally used hair-growth and hair-growth components and testosterone 5α-reductase inhibitors have side effects, such that the amount that can be incorporated is limited or the effect is insufficient. In many cases, there are problems such as poor stability in the preparation for external use for the head such as a hair restorer, or difficulty in obtaining a certain quality. Accordingly, the present invention is applicable to hair loss and alopecia caused by various causes in addition to male pattern baldness, and has an effect of preventing hair loss and hair growth and hair growth. It is an object of the present invention to provide an external preparation for a head, such as a hair nourishing agent, which is synergistically improved and highly safe for the scalp. [0007] In order to solve the above problems, various studies have been made. As a result, Agaricus blanc
zei Murill) One or more selected from mycelium extract and culture filtrate, and sage ( Salvia officinalis)
L.), hops ( Humulus lupulus L.), rosemary ( Rosmarinus officinalis L.), Hypericum ( Hyper
icum erectum Thunb.), Mentha ( Mentha arvensis L.)
var. piperascens Malinv.), peppermint (Mentha
piperita L.), chamomile ( Matricaria chamomilla )
L.), Arnica ( Arnica montana L.), Thyme ( Thymu
s vulgaris L.), one or two selected from each extract
It has been found that the combined use of more than two or more species synergistically improves hair loss prevention and hair growth and hair growth effects. Also, extracts of Agaricus blazei Murill mycelium or culture filtrate, and each extract such as sage show a sufficiently effective action at a low concentration, respectively, so that skin irritation and contact sensitization No adverse effects were observed, and both showed stable quality. [0008] Agaricus blazei Murill
Is a kind of agaric genus of the basidiomycete agaricaceae agaricaceae, also known as Kawari agaric or Himematsutake. Agaricus mushrooms are distributed in the southeastern and southern parts of North America, and in the southeastern part of Brazil, are a type of mushroom that has been used by residents for a long time. Many reports have been made on the effectiveness of this Agaricus mushroom, especially on its antitumor activity. For example,
A polysaccharide having an antitumor effect can be obtained by extracting the fruit body or mycelium of Agaricus mushroom with an aqueous solvent (JP-A-55-74797, JP-A-55-108).
292, JP-A-1-67194, JP-A-1
JP-A-67195, JP-A-6-128164, etc.), that a nucleic acid component having an antitumor effect can be obtained from the fruit body of Agaricus mushroom (JP-A-1-66127), from the hot water extraction residue of Agaricus mushroom Isolation of a protein polysaccharide having antitumor activity (JP-A-2-7863)
No. 0), that a glycoprotein having an antitumor activity can be obtained from an 85% ethanol extraction residue of Agaricus mushroom (Japanese Patent Laid-Open No. 6-9423), and that ergosterol having an antitumor activity present in the fruit body of Agaricus mushroom Derivatives (JP-A-1-246299) and the like are disclosed. In addition, as a physiological activity of Agaricus mushroom, a liver function improving agent containing an Agaricus mushroom fruit body extract as an active ingredient (Japanese Patent Laid-Open No. 2-124)
No. 829), the antiallergic effect of the Agaricus mushroom mycelium culture extract (Japanese Patent Application Laid-Open No. 1-228480), and the effect of the Agaricus mushroom fruiting body extract on the reduction of the immune function (Japanese Patent Application Laid-Open No.
-258107) and the like. further,
It has already been disclosed that the Agaricus mushroom mycelium extract or the culture filtrate has a hair root activating effect (Japanese Patent Application No. 9-1997).
366848). On the other hand, sage ( Salvia officinalis)
L.), hops ( Humulus lupulus L.), rosemary ( Rosmarinus officinalis L.), Hypericum ( Hyper
icum erectum Thunb.), Mentha ( Mentha arvensis L.)
var. piperascens Malinv.), peppermint (Mentha
piperita L.), chamomile ( Matricaria chamomilla )
L.), Arnica ( Arnica montana L.), Thyme ( Thymu
s vulgaris L.), one or two selected from each extract
More than one species are disclosed to have testosterone 5α-reductase inhibitory activity (Japanese Patent Publication No. 6-47).
554). [0010] The present inventors have now developed one or more selected from a solvent extract of Agaricus mushroom mycelium and a culture filtrate, and sage ( Salvia officinalis L.), hop ( Humul ).
us lupulus L.), rosemary ( Rosmarinus officina)
lis L.), Hypericum erectum Thun
b.), mint ( Mentha arvensis L. var. piperascens
Malinv.), Mentha Piperita L.,
Chamomile ( Matricaria chamomilla L.), Arnica ( Ar
nica montana L.) and thyme ( Thymus vulgaris L.) by combining them with one or more selected from each extract in a base for external use for the head such as a hair restorer.
The present inventors have found that the hair loss preventing action and the hair growth and hair growth actions are synergistically improved, thereby completing the present invention. DETAILED DESCRIPTION OF THE INVENTION Agaricus mushroom ( Aga) used in the present invention
ricus blazei Murill) is already widely known and has been deposited with the National Institute of Advanced Industrial Science and Technology under the deposit number of Life Science Bacteria No. 4731. As a method for cultivating the mycelium of Agaricus mushrooms, any of a solid culture method and a liquid culture method usually used for cultivation of basidiomycetes may be employed, but the latter method is preferably used from the viewpoint of productivity. . The culture medium may be any medium as long as it contains various nutrients necessary for the growth of mycelium, and an ordinary medium can be used. That is, as the carbon source, any carbon source that can be used, such as glucose, sucrose, maltose, and starch, can be used. Examples of the nitrogen source include ammonium sulfate, ammonium nitrate, and urea. Examples of natural complex nutrients include potato extract, carrot extract, malt extract, peptone, koji extract, yeast extract, yeast powder, and the like. In addition, trace elements such as inorganic salts and vitamins necessary for growth are appropriately added and used. The cultivation is usually preferably carried out under aerobic conditions, for example, a shaking culture method or an aeration stirring culture method. The stirring during the culture may be performed by reciprocating or rotating for several minutes every 24 hours, or may be performed by continuous shaking. The culture temperature is 15 ° C to 40 ° C, preferably 20 ° C to 3 ° C.
It is around 0 ° C. The pH of the medium is suitably in the range of 3.0 to 9.0, and particularly 4.5 to 7.0, and the growth is good. In addition, it is preferable not to illuminate during culturing, but 11 to 1 day a day.
Illumination for about 4 hours is possible. The number of culture days varies depending on culture conditions such as culture temperature and medium composition, but may be any period during which sufficient growth of mycelium is observed, and is usually 2 to 120 days, particularly preferably 5 to 90 days. What is necessary is just to set the period in which the mycelium production is maximized. After completion of the culture, the mycelium and the culture filtrate are separated by centrifuging or filtering the culture. Centrifugation is 100-5,000 G, preferably 800-3,000.
It can be performed by a centrifugal operation giving a gravitational acceleration of G. The filtration is performed using a membrane filter of 3.5 to 200 mesh, particularly preferably 4 to 16 mesh. When an extract is obtained from the mycelium of Agaricus mushroom, the mycelium obtained from the culture solution cultured as described above can be used as it is or after drying. As the solvent for extraction from the mycelium, a polar solvent is preferably used. For example, in addition to water, alcohols such as methanol, ethanol, isopropanol and isobutanol, glycerin, ethylene glycol, ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, propylene glycol, propylene glycol monomethyl ether, propylene glycol monoethyl ether, triglycol Polyhydric alcohols such as ethylene glycol, 1,3-butylene glycol and hexylene glycol or derivatives thereof, ethers such as ethyl ether and propyl ether, esters such as ethyl acetate and butyl acetate, ketones such as acetone and ethyl methyl ketone One or a mixture of two or more solvents selected from the above-mentioned types can be used. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used. Further, as an extraction method, a method of extracting by impregnating with an extraction solvent at room temperature, cooling or heating, a method of extracting using a distillation method such as steam distillation, and a method of directly compressing raw Agaricus mushroom mycelium. Examples of such methods include a squeezing method for obtaining an extract, and these methods are used alone or in combination of two or more. The ratio of Agaricus mushroom mycelium to the solvent at the time of extraction is not particularly limited, but the solvent is 0.5 to 1,000 times by weight with respect to Agaricus mushroom mycelium 1, especially the extraction operation and efficiency. In view of this, the weight is preferably 0.5 to 100 times by weight.
The extraction temperature is conveniently in the range from about 5 ° C. under normal pressure to the boiling point of the solvent or lower, and the extraction time varies depending on the extraction conditions such as the extraction temperature, but is preferably in the range of 2 hours to 2 weeks. . The agaricus mushroom mycelium extract thus obtained can be used as it is, but it can be fractionated, deodorized and deodorized within a range that does not impair the hair root activating effect.
A purification operation such as decolorization and concentration can be used.
These extracts, purified products and fractionated products can be made into powder by concentration to dryness or freeze-drying, and further solubilized or suspended in a solvent such as purified water or the like, and a hair nourishing agent or other external preparation for the head. Can also be added. On the other hand, the filtered mycelium culture filtrate that has been filtered can be directly added to an external preparation for the head. However, purification, such as fractionation, deodorization, decolorization, and concentration, is also performed within a range that does not lose the hair root activating action. It can also be used with additional operations. These culture filtrates, purified products, and fractionated products can be dried by removing the solvent therefrom, and can be further solubilized or suspended in a solvent such as purified water, or in the form of an emulsion. Can be added to the external preparation for the head. From the Agaricus mushroom mycelium extract and the mycelium culture filtrate, one or more are selected and blended into an external preparation for the head such as a hair restorer. The amount of the compound varies depending on the extraction method and other preparation methods, but is preferably in the range of 0.0001 to 10% by weight in consideration of bioavailability and influence on the preparation. On the other hand, sage ( Salvia officinalis L.)
Such plant extracts are obtained by immersing these plants in an extraction solvent. For extraction, each part such as a flower, a leaf, a stem, a root and the like of each plant and a whole plant can be used. These plants may be subjected to an extraction treatment as it is, but in order to increase the extraction efficiency, it is preferable to extract after shredding, pulverizing or the like, or to homogenate in an extraction solvent. As the extraction solvent, a polar solvent is preferable. In addition to water, lower alcohols such as methanol, ethanol, propanol, isopropanol and butanol, propylene glycol, dipropylene glycol,
Preferred are polyhydric alcohols such as 1,3-butylene glycol and glycerin, ethers such as ethyl ether and propyl ether, esters such as ethyl acetate and butyl acetate, and ketones such as acetone and ethyl methyl ketone. And one or more of these are selected and used. Phosphate buffer, physiological saline, and phosphate buffered saline can also be used favorably. Extraction with ethanol is particularly preferred from the viewpoint of testosterone 5α-reductase inhibitory activity. 5-5 as extraction temperature
About 0 ° C. and an extraction time of about 4 hours to about 1 week are appropriate. The above-mentioned plant extract may be added as it is to a base for external use for the head such as a hair tonic, etc., but the concentrated and dried one may be dissolved again in water or a polar solvent, or testosterone may be added.
It may be added after performing a purification treatment such as decolorization, deodorization, and desalting to the extent that 5α-reductase inhibitory activity is not impaired. After concentration and drying, or after performing a purification treatment,
It may be freeze-dried and added as a powder. In the present invention, 1% of the above plant extract is used.
A seed or two or more kinds are selected and blended in the external preparation for head. The amount to be blended also varies depending on the testosterone 5α-reductase inhibitory activity of the extract, but is 0.0001 to 10
It is appropriate to set it to about% by weight. In the external preparation for head according to the present invention, one or more selected from the active ingredients of Agaricus mushroom mycelium extract and culture filtrate, and sage ( Salvia o.
fficinalis L.), hops ( Humulus lupulus L.), rosemary ( Rosmarinus officinalis L.), hypericum ( Hypericum erectum Thunb.), peppermint ( Mentha a )
rvensis L. var. piperascens Malinv.) , peppermint (Mentha piperita L.), chamomile (Matricaria c
hamomilla L.), Arnica ( Arnica montana L.), Thymus ( Thymus vulgaris L.) In addition to one or more selected from each extract, in order to further enhance hair growth and hair growth promotion effect, Estrogen, such as estrone, estradiol, ethinyl estradiol, vitamin E and its derivatives, assembly extract, garlic extract, carrot extract, aloe extract, cepharanthin, carpronium chloride, minoxidil, etc., peripheral blood flow promoters, capsicum tincture , Cantaris tincture, Showa tincture,
Topical stimulants such as peppermint oil, l-menthol and camphor; keratolytic agents such as resorcinol, salicylic acid and lactic acid; antiseborrheic agents such as pyridoxine and its derivatives; zinc pyrithione;
Fungicides such as benzalkonium chloride, benzethonium chloride, chlorhexidine, hinokitiol, pantothenic acid and its derivatives, placental extracts, hair root activators such as biotin and pentadecanoic acid glyceride, glycyrrhizic acid and its derivatives, β-glycyrrhetinic acid and its derivatives , Allantoin, azulene, ε-aminocaproic acid, hydrocortisone, etc., vitamins such as vitamins A, B ₂ , B ₁₂ , D, cysteine, serine, methionine, leucine,
Amino acids such as tryptophan can be simultaneously added. Further, as long as the effects of the present invention are not impaired, oils and fats, lower alcohols, polyhydric alcohols, surfactants, humectants, fungicides and fungicides, ultraviolet absorbers, fragrances,
Raw materials and additives generally used in an external preparation for the head such as a hair nourishing agent such as pigments and pigments can be contained. The external preparation for the head according to the present invention can be provided in the form of a lotion, emulsion, gel, cream, ointment or the like. It can also be provided as a head cosmetic in the form of hair lotion, hair tonic, hair milk, hair gel, hair cream, hair pack, hair treatment, hair shampoo, hair rinse. EXAMPLES Further, the features of the present invention will be described in detail with reference to examples. First, examples of production of Agaricus mushroom mycelium extract, Agaricus mushroom mycelium culture filtrate, and plant extracts such as sage used in Examples of the present invention are shown below. Production Example 1 Agaricus mushroom mycelium extract 1 An Agaricus mushroom mycelium extract 1 was obtained by the method described in Example 1 of JP-B-61-47518. That is, a pH consisting of 4 g of glucose, 4 g of yeast extract, 10 g of malt extract and 1,000 ml of purified water.
The medium of 5.5 was inoculated with Agaricus mushroom mycelium cultured on an agar medium, and pre-cultured at 30 ° C. for 30 days with occasional stirring and standing. Then, glucose 20g, yeast powder 5
g, an antifoaming agent of 20 ppm and purified water of 1,000 ml, and 2,000 ml of a main culture medium having a pH of 5.0 were inoculated with 200 ml of the precultured medium, and cultured at 30 ° C. for 30 days with a reciprocating shaker. The mycelium was separated from the culture solution by centrifugation, purified water was added 7 times by weight to the mycelium, and the
Heat extraction was performed at 2 ° C. for 2 hours. The supernatant obtained by centrifuging the extract was subjected to Agaricus mushroom mycelium extract 1 (Production Example 1).
And [Production Example 2] Agaricus mushroom mycelium extract 2 The above Agaricus mushroom mycelium extract 1 was concentrated to 容量 volume, ethanol was added to the same volume, and the mixture was allowed to stand at 4 ° C. for 24 hours to form a precipitate. I let it. The precipitate was collected by centrifugation, washed with acetone and then with ether, and dried to obtain a light brown powder. Agaricus mushroom mycelium extract 2 (Production Example 2)
And Production Example 3 Agaricus mushroom mycelium extract 3 An Agaricus mushroom mycelium extract 3 was obtained by the method described in Example 2 of JP-B-61-47518. That is, agaricus mycelia cultured on an agar medium was inoculated into a pH 5.0 medium consisting of 20 g of glucose, 5 g of yeast extract and 1,000 ml of purified water, and then inoculated into the medium.
Preculture was carried out by standing at ℃ for 3 weeks. 500 ml of this preculture
With 50 g of glucose, 10 g of yeast powder and 20 pp of an antifoaming agent
m and 1,000 ml of purified water were inoculated into 3,000 ml of a main culture medium having a pH of 5.0, and cultured under conditions of a culture temperature of 30 ° C., an aeration rate of 0.5 VVM, and a stirring speed of 200 to 300 rpm. After culturing for 10 days, the mycelium was separated and collected by filtration. 3,000 ml of purified water was added to the mycelium, and the mixture was heated and extracted at 50 ° C. for 1 hour. After cooling, 10,000
The mixture was centrifuged at 0 rpm for 30 minutes, and the supernatant was used as Agaricus mushroom mycelium extract 3 (Production Example 3). Production Example 4 Agaricus mushroom mycelium extract 4 Agaricus mushroom mycelium extract 3 was concentrated under reduced pressure using a rotary evaporator to 1,000 ml. 1,000 ml of ethanol was added thereto, and the mixture was allowed to stand at 4 ° C. for a day and night to form a precipitate. Next, the precipitate was recovered, washed with acetone and ether, and dried to obtain a pale gray-white powder. This was further dissolved in 500 ml of purified water, dialyzed against 5,000 ml of purified water with a dialysis tube, and the resulting precipitate was removed by centrifugation. The supernatant was freeze-dried to obtain a white powder. Agaricus mushroom mycelium extract 4 (Production Example 4). [Preparation Example 5] Agaricus mushroom mycelium culture filtrate 1 At the time of preparation of Preparation Example 1, the culture liquid after removing the mycelia from the main culture liquid of the Agaricus mushroom mycelium was used as the Agaricus mushroom mycelium culture filtrate 1 ( Production Example 5) was obtained. Production Example 6 Agaricus mushroom mycelium culture filtrate fraction 1 Agaricus mushroom mycelium culture filtrate 1 was concentrated to 1/6 volume with a rotary evaporator, and an equal volume of ethanol was added thereto. The mixture was allowed to stand overnight and a precipitate was formed.
The precipitate was collected by centrifugation, washed with acetone and ether, and then dried to obtain a light brown powder, which was used as Agaricus mushroom mycelium culture filtrate fraction 1 (Production Example 6). [Preparation Example 7] Agaricus mushroom mycelium culture filtrate 2 At the time of preparation of Preparation Example 3, the culture liquid after removing the mycelium from the Agaricus mushroom mycelium main culture solution was used as the Agaricus mushroom mycelium culture filtrate 2 (manufactured). Example 7). Production Example 8 Agaricus mushroom mycelium culture filtrate fraction 2 Agaricus mushroom mycelium culture filtrate 2 was concentrated to 1/10 volume by a rotary evaporator, and an equal volume of ethanol was added thereto. To generate a precipitate. The precipitate was collected by centrifugation, washed with acetone and ether, and dried to obtain a light brown powder. This is purified water 30
0 ml, purified water 3,000m with dialysis tube
After dialysis against 1 and removing the resulting precipitate by centrifugation, the supernatant was freeze-dried to obtain a white powder, which was used as a filtrate fraction 2 of Agaricus mushroom mycelium culture (Production Example 8). [Production Examples 9 to 26] The plants shown in the second column of Table 1 were minced, and immersed in each solvent shown in the third column of Table 1 at 20 ° C. for 3 days to perform extraction. The filtrate obtained by filtering the extract was further treated as shown in the fourth column of Table 1 to obtain extracts such as sage (Production Examples 9 to 26). [Table 1] Using the above-mentioned production examples, the hair growth promoting effect of a combined use of an agaricus mushroom mycelium extract or a culture filtrate with a plant extract such as sage was examined. C57 / Blac
The back of the k-mouse was shaved with a clipper to a size of 2 × 4 cm, treated with a depilatory foam to completely remove hair, and after 24 hours, 0.1 ml of each sample solution shown in Table 2 was used once a day. Each was continuously applied for 20 days. All hairs that grew on the last day of the test were extracted, and their total weight was measured. The test was performed with 10 animals per group, and the average value was calculated and shown in Table 3. At that time, a group to which only a 50% by weight aqueous ethanol solution of a solvent was applied was set as a control. [Table 2] [Table 3] As is clear from Table 3, the Agaricus mushroom mycelium extract or the culture filtrate of Production Examples 1 to 8 and the plant extract of Production Examples 9 to 26 were compared with the control in the group applied alone. Although hair growth was significantly enhanced in comparison, when these were used in combination, the amount of hair growth was significantly increased. [Examples 1 to 8] Hair Nourishing Lotion A hair nourishing lotion was prepared using the Agaricus mushroom mycelium extract or the culture filtrate and the plant extract shown in Table 4 according to the following formulation. (Prescription) (1) Ethanol 50.00 (% by weight) (2) Vitamin B ₆ 0.10 (3) Agaricus mushroom mycelium extract 0.05 or culture filtrate (4) Plant extract 0.50 (5) 1,3-butylene glycol 2.50 (6) Fragrance 0.10 (7) Purified water 46.75 Production method: (1) to (6) are sequentially added to (7) and mixed to make uniform. [Table 4] A use test was conducted for Examples 1 to 8 to evaluate the hair-growth effect. At that time, in the above Examples 1, 2, 5, and 6, the agaricus mushroom mycelium extract or the culture filtrate was replaced with purified water to obtain Comparative Example 1, Comparative Example 2, Comparative Example 5, and Comparative examples 6, Comparative examples 4, Comparative examples 7 and 8 were obtained by replacing the plant extract with purified water in Comparative example 6, Example 3, Example 4, Example 7 and Example 8. Further, Agaricus The same test was performed as Comparative Example 9 except that both the mushroom mycelium extract or the culture filtrate and the plant extract were replaced with purified water. In the use test, 20 male and female panelists who were worried about thinning hair and hair loss were treated as a group, and each of the examples and comparative examples was used blindly twice a day for 6 consecutive months. Evaluation was made on the status of improvement of hair loss symptoms and the presence or absence of irritation during use. The state of improvement of the hair thinning and hair loss symptoms was evaluated by scoring the condition of the hair before and after the start of the use test according to the criterion shown in Table 5 by photography and scored, and the average value of 20 subjects was calculated. Regarding irritation during use, "I do not feel; 0 points",
Points were scored as "feel slightly; 1 point", "slightly felt; 2 points", "clearly felt; 3 points", and "very much felt; 4 points", and the average value of 20 people was obtained. The above results are summarized in Table 6. [Table 5] [Table 6] As is clear from Table 6, in the group using the examples of the present invention, in each group, most of the panelists showed hair growth and hair growth more than the whole raw hair, and the panelers who saw hard hair were further observed. And a very good hair loss symptom and improvement of thinning hair were observed. In contrast,
Comparative Examples 1 to 8 containing only one of the Agaricus mushroom mycelium extract or culture filtrate, or the plant extract
In the application group, improvement of hair loss symptoms and thinning hair was observed,
The degree was smaller than that in each of the applied groups.
In the group to which Comparative Example 9 was applied, which did not contain any of the Agaricus mushroom mycelium extract, the culture filtrate, and the plant extract, no significant change was observed in the hair condition. Regarding the feeling of irritation during use, no particularly problematic feeling of irritation was observed in any of Examples 1 to 8 and Comparative Examples 1 to 9 of the present invention. Next, the formulation of another embodiment of the present invention will be described. Example 9 Head Cream (1) Liquid Paraffin 15.00 (% by weight) (2) Vaseline 15.00 (3) Beeswax 2.00 (4) Polyoxyethylene (50E.O.) Hardened castor oil 3.00 (5) Glycerin 5.00 (6) Carboxyvinyl polymer 0.10 (7) Xanthan gum 0.10 (8) Disodium ethylenediaminetetraacetate 0.10 (9) Purified water 59.10 (10 ) Sodium hydroxide 0.05 (11) Agaricus mushroom mycelium extract 1 (Production Example 1) 0.25 (12) Thyme extract (Production Example 25) 0.15 (13) Sage extract (Production Example 9) 0.15 Production method; heat and dissolve the oil phase of (1) to (3) to 80 ° C. On the other hand, the aqueous phase components (4) to (9) are mixed and dissolved by heating to 80 ° C. The oil phase was added to the water phase while stirring, and the mixture was uniformly emulsified by a homogenizer. After cooling, (10) was added at 30 ° C., and after mixing, (11) to (13) were previously mixed, dissolved, and added. Mix and homogenize. Example 10 Head Gel (1) Carboxyvinyl polymer 0.7 (% by weight) (2) Polyvinylpyrrolidone 2.0 (3) Glycerin 5.0 (4) Sodium hydroxide 0.2 (5) Ethanol 20.0 (6) Disodium ethylenediaminetetraacetate 0.1 (7) Purified water 70.8 (8) Agaricus mushroom mycelium extract 2 (Production Example 2) 0.2 (9) Hop extract (Production Example 11) 1.0 Production method: (1) is dispersed in a part of (3) and (7). (2),
Dissolve (4) to (6) in the remainder of (7) and add (8) and (9)
Is mixed, dissolved and added in advance, and mixed and homogenized. Example 11 Set lotion (1) Polyvinylpyrrolidone / vinyl acetate copolymer 5.00 (% by weight) (2) Methyl paraoxybenzoate 0.10 (3) Fragrance 0.10 (4) Ethanol 30 0.00 (5) Polyoxyethylene / polyoxypropylene modified 0.50 Dimethyl polysiloxane (6) Glycerin 2.00 (7) Agaricus mushroom mycelium extract 3 (Production Example 3) 0.01 (8) Rosemary extraction (Production Example 13) 0.25 (9) Purified water 62.04 Production method: (1) to (3) are sequentially added to (4) and dissolved. On the other hand (5)
The aqueous phases of (9) to (9) are mixed and dissolved, and added to the above solution, mixed, and made uniform. Example 12 Hair Liquid (1) Ethanol 50.00 (% by weight) (2) Polyoxypropylene (40 PO) butyl ether 20.00 (3) Polyoxyethylene (50 EO) cured Castor oil 1.00 (4) Methyl paraoxybenzoate 0.15 (5) Agaricus mushroom mycelium extract 4 (Production Example 4) 0.10 (6) Hypericum extract (Production Example 15) 0.50 (7) Mint extract (Production Example 17) 1.00 (8) Fragrance 0.10 (9) Blue No. 1 (1% aqueous solution) 0.10 (10) Purified water 27.05 Production method: (2)-(1) The components of (10) are sequentially added and dissolved to make them uniform. Example 13 Water Grease (1) Carboxyvinyl polymer 0.5 (% by weight) (2) Glycerin 50.0 (3) Purified water 38.5 (4) Polyoxyethylene (20E.O.) Octyldodecyl 0.2 Ether (5) Sodium hydroxide 0.1 (6) Ethanol 10.0 (7) Agaricus mushroom mycelium culture filtrate 1 (Production Example 5) 0.2 (8) Agaricus mushroom mycelium culture filtrate Painting 2 (Production Example 8) 0.2 (9) Chamomile extract (Production Example 21) 0.2 (10) Fragrance 0.1 Production method: (1) is dispersed in (2) and part of (3) I do. (4),
(6) to (10) are dissolved and added to the remainder of (3), and then (5) is added to thicken. [Example 14] Hair foam (stock solution formulation) (1) Silicone oil 5.00 (% by weight) (2) Polyoxyethylene (50E.O.) hydrogenated castor oil 1.00 (3) Dipropylene glycol 7.00 (4) Cationized cellulose 3.00 (5) Purified water 67.00 (6) Ethanol 15.00 (7) Agaricus mushroom mycelium culture filtrate fraction 1 (Production Example 6) 0.25 (8 ) Common peppermint extract (Production Example 19) 1.50 (9) Methyl paraoxybenzoate 0.15 (10) Fragrance 0.10 (filling formulation) Stock solution 90.0 Liquefied petroleum gas 10.0 Production method: (1) Add to the melts of (2) and (3) and emulsify uniformly with a homogenizer. This is added to and dissolved in (4), (6) to (10) dissolved in (5), and mixed to make a uniform solution. Filling is performed by filling a can with a stock solution, mounting a valve, and then filling with liquefied petroleum gas. Example 15 Hair Shampoo (1) Polyoxyethylene (3E.O.) Lauryl Sulfate 30.00 (wt%) Ester Sodium Salt (30 wt% aqueous solution) (2) Lauryl Sulfate Sodium Salt 00 (30% by weight aqueous solution) (3) Coconut oil fatty acid diethanolamide 4.00 (4) Glycerin 1.00 (5) Sodium dehydroacetate 0.20 (6) Disodium ethylenediaminetetraacetate 0.25 (7) Purified water 53.55 (8) Agaricus mushroom mycelium culture filtrate 2 (Production Example 7) 0.50 (9) Arnica extract (Production Example 23) 0.20 (10) Thyme extract (Production Example 26) 0.20 ( 11) Fragrance 0.10 Production method: (7) is heated to 70 ° C, (1) to (6) are added and uniformly dissolved, then cooled, and (8) to (11) are added and mixed at 30 ° C. ,
Be uniform. Example 16 Hair rinse (1) Silicone oil 3.00 (% by weight) (2) Liquid paraffin 1.00 (3) Cetanol 1.50 (4) Stearyl alcohol 1.00 (5) Stearyl trimethyl chloride Ammonium 0.70 (6) Glycerin 3.00 (7) Methyl paraoxybenzoate 0.20 (8) Purified water 88.92 (9) Agaricus mushroom mycelium extract 2 (Production Example 2) 0.02 (10) Agaricus mushroom mycelium culture filtrate fraction 1 (Production Example 6) 0.02 (11) Sage extract (Production Example 10) 0.02 (12) Hop extract (Production Example 12) 0.02 (13) Thyme Extract (Production Example 26) 0.50 (14) Fragrance 0.10 Production method: The oil phase components (1) to (4) and the aqueous phase components (5) to (8) were mixed and dissolved, Heat to ° C. The oil phase is added to the water phase to emulsify, and after cooling, (9) to (14) are previously mixed, dissolved and added at 30 ° C., and mixed and homogenized. The above-described embodiments 1 to 16 of the present invention
In each case, the stability was good, and even when stored at 25 ° C. for 6 months, no change in the state such as a change in color and odor, phase separation, appearance of precipitates and sediments was observed. Also, as a result of a 48-hour back obstruction sticking test by a male panelist, no skin irritation was observed. As described in detail above, the present invention can be applied to hair loss and alopecia caused by various causes in addition to male pattern baldness, and has an effect of preventing hair loss and hair growth and hair growth. Was synergistically improved and a highly safe external preparation for the head for the scalp could be provided.

──────────────────────────────────────────────────続 き Continuation of the front page (56) References JP-A-11-193220 (JP, A) JP-A-1-228480 (JP, A) JP-A-7-316026 (JP, A) JP-A 9-1998 328415 (JP, A) JP-A-9-221413 (JP, A) JP-A-8-310923 (JP, A) (58) Fields investigated (Int. Cl. ⁷ , DB name) A61K 7/00-7 / 50 JICST file (JOIS) CA (STN)

Claims (1)

(57) [Claims] (Claim 1) Agaricus blazei Muril
l) Mycelium extract and Agaricus blaz
ei Murill) One or two selected from mycelium culture filtrate
More than species, sage ( Salvia officinalis L.), hop ( Humulus lupulus L.), rosemary ( Rosmarinus o.)
fficinalis L.) and Hypericum ( Hypericum erectum T)
hunb.), peppermint (Mentha arvensis L. var. piperasce
ns Malinv.), Mentha piperita
L.), chamomile ( Matricaria chamomilla L.), arnica ( Arnica montana L.), thyme ( Thymus vulgaris )
An external preparation for the head, comprising one or more selected from the extracts of L.).