JP3382142B2 - Hair restorer and hair cosmetic - Google Patents

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a hair nourishing agent and hair which can activate hair follicles to promote hair growth and hair growth, and improve the condition of the scalp to prevent and improve dandruff and itch. For cosmetics. More specifically, the present invention relates to a hair nourishing agent and a hair cosmetic composition containing an extract of Agaricus blazei Murill mycelium or a culture filtrate.

[0002]

2. Description of the Related Art Hair cosmetics for the purpose of preventing and improving alopecia have been developed. Of the alopecia, the proportion of male pattern baldness is high, so the application of components with anti-androgen action has been investigated, and it competitively inhibits the binding of the active testosterone, dihydrotestosterone, to the receptor. Also, inhibitors of testosterone 5α-reductase, which is an enzyme that catalyzes the conversion of testosterone to dihydrotestosterone, have been disclosed. The former is cyproterone acetate, and the latter is the androstanone derivative, bicycloheptenone derivative, phenoxybutane derivative, tocopherylquinone, tropolone derivative, ubiquinone, etc., and extracts of many plants such as Lamiaceae plants and Asteraceae plants. Is mentioned.

Further, 2,4-diamino-6-piperidinopyrimidine-3-oxide (minoxidil), cepharanthin,
Activates hair follicles including hair mother cells such as vitamin E derivatives, carpronium chloride, etc., which have a blood circulation promoting effect on the scalp, and adenosine triphosphate, urogastrone, baicalein, pantethein-S-sulfonic acid, odd chain fatty acid derivatives, etc. The application of those having action is also under study.

However, the onset of alopecia is not only caused by testosterone-dependent androgenetic alopecia, but also due to various causes such as aging, malnutrition, and stress. For such alopecia other than male pattern, the effect of the component having an antiandrogen action cannot be expected, and among the components having an antiandrogen action described above, there is a concern that side effects may occur or a cosmetic base There were many cases where the stability in the agent was poor or the action and effect were insufficient. Furthermore, it is difficult to obtain a certain quality of natural products such as plant extracts, and moreover, there are many unfavorable colors and odors when blended into cosmetics.

On the other hand, among those reported to have a scalp blood circulation promoting action and a hair follicle activating action, few have sufficient action and effect at a low concentration, and some have problems in stability and safety. Existed.

[0006]

Therefore, in the present invention, a hair nourishing agent and a hair cosmetic composition capable of promoting hair growth and hair growth in thinning hair and alopecia caused by various causes by activation of hair follicles are obtained in the present invention. It was intended.

[0007]

In order to solve the above-mentioned problems, the present inventors screened substances having an effective hair growth and hair growth promoting action using the hair follicle activating action as an index. As a result, Agaricus blaz
ei Murill) extract of mycelium and Agaricus (Agaricus
blazei Murill) We found an extremely high hair follicle activating effect in the mycelium culture filtrate, and by applying this, it has excellent hair growth and hair growth promoting effects, good formulation stability and safety, and a good usability. It was possible to obtain an excellent hair nourishing agent and hair cosmetic, and the present invention was completed.

Agaricus blazei Murill
Is a kind of agaricaceae, agaricaceae, agaricaceae, and is also called Kawariharatake or Himematsutake. Agaricus edulis are distributed in the southeastern part of South America and South America, and in the southeastern part of Brazil, it is a kind of edible mushrooms that residents have been eating for a long time. Many reports have been made on the effectiveness of Agaricus edulis, especially regarding its antitumor activity. For example, a polysaccharide having an antitumor effect can be obtained by extracting the fruiting body or mycelium of Agaricus edulis with an aqueous solvent (JP-A-55-74797, JP-A-55-108292).
Japanese Patent Laid-Open No. 1-67194 and Japanese Patent Laid-Open No. 1-67194
195, JP-A-6-128164, etc.), that a nucleic acid component having an antitumor effect can be obtained from the fruiting body of Agaricus edulis (JP-A-1-66127), and anti-tumor activity from hot water extraction residue of Agaricus edulis. Isolation of a protein polysaccharide having tumor activity (Japanese Patent Laid-Open No. 2-78630),
Glycoprotein having antitumor activity can be obtained from 85% ethanol extraction residue of Agaricus edulis (JP-A-6-942).
3), an ergosterol derivative having antitumor activity present in the body of Agaricus edulis (JP-A-1-246).
No. 299) is disclosed. As a physiological activity of Agaricus edulis, a liver function improving agent containing an Agaricus edulis extract as an active ingredient (JP-A-2-124829) and an anti-allergic effect of Agaricus edulis mycelium culture extract (JP-A-1-228480) JP-A-7-2581.
No. 07 gazette) and the like have been reported. However, Agaricus (Agaricus blazei Murill) mycelium extract and Agaricus (Agaricus blazei Murill) mycelium culture filtrate, to have a significant hair follicle activating action is newly found by this present inventors It is a thing.

[0009]

[Operation] Agaricus used in the present invention
blazei Murill) mycelium extract and Agaricus mushroom ( Agari
The hair follicle activating action of the cus blazei Murill) mycelium culture filtrate is shown below. Agaricus (Agaricus blazei Murill) extract of mycelium and Agaricus (Agaricus blazei Muril
l) As the mycelium culture filtrate, those of Production Examples 1 to 8 described later were used.

The hair follicle activating effect was evaluated using cultured cells derived from mouse hair follicles. First, the hair follicles separated and collected from the skin of a 4-day-old ICR mouse were washed and trypsinized,
Seed on a collagen plate at 1 × 10 ⁵ cells per well, and streptomycin 100 μg / m 2.
1, 100 IU / ml of penicillin and 10% by volume of fetal bovine serum were added to Dulbecco's modified basal medium (DMEM), and the cells were cultured for 24 hours in the air having a carbon dioxide partial pressure of 5%. Then, the medium was treated with insulin 5 μg / ml, epidermal growth factor (EGF) 5 ng / ml, hydrocortisone 0.5 μg.
/ Ml, calcium ion 0.03 mM, and Agaricus blazei Murill mycelium extract of each production example, etc. were exchanged with MCDB153 high amino acid medium containing 0.002-0.1% by weight, and incubated for 4 days. In the eye, 2-
(4,5-Dimethyl-2-thiazolyl) -3,5-diphenyltetrazolium bromide (MTT) was exchanged with the above medium containing 0.4 mg / ml and cultured at 37 ° C. for 2 hours. The formazan resulting from ring opening was extracted with 200 μl of 2-propanol and measured by absorbance at 560 nm. In addition, Agaricus bl
Azei Murill) mycelium extract was not treated and treated in the same manner as a control, and the activation index represented by the absorbance of the control as 100.0 was determined and is shown in Table 1.

[0011]

[Table 1] In Table 1, Agaricus blazei Murill
Extract of mycelium or Agaricus blazei Mur
ill) When the culture filtrate of mycelium was added in an amount of 0.002 to 0.1% by weight, a significant increase in the activation index was observed in a concentration-dependent manner, and addition of 0.002% by weight or more resulted in significant hair follicle activity. Was recognized. In particular, Production Example 2, Production Example 4, Production Example 6
Also, in Production Example 8, a high activation effect was recognized. No cytotoxicity to hair follicles was observed at any concentration.

[0012] Also, Agaricus blazei Muril
l) Mycelium extract and Agaricus blazei M
(urill) mycelium culture filtrate improved the condition of the scalp at a concentration almost the same as the concentration showing activation of hair follicles, and also exhibited the action of preventing and improving dandruff and itch.

[0013]

BEST MODE FOR CARRYING OUT THE INVENTION In the present invention, Agaricus blister ( Agaricus blister) used for obtaining a mycelium extract and a culture filtrate is used.
azei Murill) has already been widely known, and has a deposit number at the Institute of Biotechnology, Institute of Biotechnology, Institute of Industrial Science, Life Science Research Institute No. 47.
Deposited as No. 31.

As a method for cultivating the mycelium of Agaricus edulis, either a solid culture method or a liquid culture method usually used for culturing basidiomycetes may be adopted, but the latter method is preferably used from the viewpoint of productivity. . The culture medium may be any ordinary medium as long as it contains various nutrients necessary for the growth of mycelium. That is, any carbon source that can be assimilated, such as glucose, sucrose, maltose, or starch, can be used as the carbon source. As the nitrogen source, for example, ammonium sulfate, ammonium nitrate, urea, etc., and as the natural complex nutrient source, for example, potato extract, carrot extract, malt extract, peptone, koji extract, yeast extract, yeast powder, etc. can be used. In addition, trace elements such as inorganic salts and vitamins necessary for growth are appropriately added and used.

The culture is usually preferably carried out under aerobic conditions, and for example, the shaking culture method or aeration stirring culture method is used. The stirring during the culture may be performed by reciprocal shaking or rotary shaking every 24 hours for several minutes, or continuous shaking may be used. The culture temperature is 15 ° C to 40 ° C, preferably 20 ° C to 3
It is around 0 ° C. The pH of the medium is appropriately in the range of 3.0 to 9.0, and particularly 4.5 to 7.0, the growth is good. In addition, it is preferable not to illuminate during culturing,
Illumination for about 14 hours is possible.

The number of culture days varies depending on the culture conditions such as culture temperature and medium composition, but it may be any period as long as sufficient growth of mycelia is observed, usually 2 to 120 days, particularly preferably 5 to 90 days, It suffices to set a period in which the mycelium production amount is maximum.

After completion of the culture, the mycelium and the culture filtrate are separated by centrifuging or filtering the culture solution. Centrifugation is 100 to 5,000 G, preferably 800 to 3,000
It can be performed by a centrifugal operation that gives G's gravitational acceleration. The filtration is carried out using a membrane filter having 3.5 to 200 mesh, and particularly preferably 4 to 16 mesh.

When the extract is obtained from the mycelium of Agaricus edulis, the mycelium obtained from the culture cultivated as described above can be used as it is or after drying. A polar solvent is preferably used as the extraction solvent from the mycelium. For example, in addition to water, alcohols such as methanol, ethanol, isopropanol and isobutanol, glycerin, ethylene glycol, ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, propylene glycol, propylene glycol monomethyl ether, propylene glycol monoethyl ether, tri Polyhydric alcohols such as ethylene glycol, 1,3-butylene glycol and hexylene glycol or their derivatives, ethers such as ethyl ether and propyl ether, esters such as ethyl acetate and butyl acetate, ketones such as acetone and ethyl methyl ketone. One or more mixed solvents selected from the group can be used. Alternatively, physiological saline, phosphate buffer, phosphate buffered saline, etc. may be used.

Further, as an extraction method, a method of impregnating an extraction solvent at room temperature, cooling or heating for extraction, a method of extraction by using a distillation method such as steam distillation, and a raw Agaricus mycelium mycelium are directly squeezed. A squeezing method or the like for obtaining an extract is exemplified, and these methods are used alone or in combination of two or more for extraction.

The ratio of the Agaricus mycelium mycelium to the solvent at the time of extraction is not particularly limited, but the solvent is 0.5 to 1,000 times the weight of the Agaricus mycelium mycelium, particularly the extraction operation and efficiency. From the point of, 0.5 to 100 times by weight is preferable.
Further, the extraction temperature is conveniently in the range of about 5 ° C. to the boiling point of the solvent or less under normal pressure, and the extraction time is preferably in the range of 2 hours to 2 weeks, although it depends on the extraction conditions such as the extraction temperature. .

The extract of Mycelia agaricus mycelia thus obtained can be used as it is, but the fractionation, deodorization,
It is also possible to use by adding purification operations such as decolorization and concentration.
These extracts, purified products and fractions can be made into powder by concentration to dryness or freeze-drying, and further solubilized or suspended in a solvent such as purified water to prepare a hair nourishing agent or a hair cosmetic composition. It can also be added.

On the other hand, the filtered mycelium culture filtrate can also be blended as it is with a hair nourishing agent, etc. However, purification operations such as fractionation, deodorization, decolorization and concentration are also performed within a range that does not lose the hair follicle activating action. It can also be used in addition. These culture filtrates, their purified products, and fractionated products can also be made into a dry solid by removing the solvent from them, and are further solubilized or suspended in a solvent such as purified water, or in the form of an emulsion. Can be added to hair restorers and the like.

The amount of these Agaricus edulis mycelium extract and mycelium culture filtrate to be added to the hair nourishing agent and the hair cosmetic composition is
Considering the bioavailability and the influence on the preparation, it is desirable that the concentration range is 0.001 to 20% by weight.

The hair nourishing agent and hair cosmetic composition according to the present invention are
It can be provided in the form of lotions, emulsions, gels, creams, ointments, etc., and is a hair nourishing agent in the form of hair lotion, hairnic, hair milk, hair gel, hair cream, hair pack, hair treatment, hair shampoo, hair rinse. And a cosmetic for hair.

Further, the hair nourishing agent and the hair cosmetic composition according to the present invention include oils and fats, as long as the characteristics of the present invention are not impaired.
Contains lower alcohols, polyhydric alcohols, surfactants, moisturizers, cell activators, bactericides, antiseptics, UV absorbers, fragrances, etc., which are usually used in hair nourishing agents and cosmetics for hair. be able to.

[0026]

EXAMPLES First, an example of producing the agaricus mushroom mycelium extract and agaricus mushroom mycelium culture filtrate used in the examples of the present invention is shown below.

[Production Example 1] Agaricus mycelia extract 1 was obtained by the method described in Example 1 of Japanese Patent Publication No. 61-47518. That is, pH consisting of 4 g of glucose, 4 g of yeast extract, 10 g of malt extract and 1,000 ml of purified water.
The 5.5 medium was inoculated with the agaricus mushroom mycelium cultured in an agar medium, and precultured at 30 ° C. for 30 days while still standing with occasional stirring. Then, 200 ml of the precultured medium was inoculated into 2,000 ml of the main culture medium of pH 5.0 consisting of 20 g of glucose, 5 g of yeast powder, 20 ppm of defoaming agent and 1,000 ml of purified water, and the medium was reciprocally shaken at 30 ° C. For 30 days. The mycelium was separated from the culture solution by centrifugation, and 7 times by weight of purified water was added to the mycelium.
Heat extraction was performed at 5 ° C. for 2 hours. The supernatant obtained by centrifuging the extract was used as Agaricus edulis mycelium extract 1 (Production Example 1).

[Production Example 2] Agaricus mycelia mycelium extract 2 The above Agaricus mycelia mycelium extract 1 was concentrated to 1/2 volume, an equal volume of ethanol was added, and the mixture was left standing overnight at 4 ° C to form a precipitate. Let The precipitate was collected by centrifugation, washed with acetone and then with ether, and dried to obtain a light brown powder. This is agaricus mushroom mycelium extract 2 (Production Example 2)
And

[Production Example 3] Agaricus mycelia extract 3 was obtained by the method described in Example 2 of Japanese Patent Publication No. 61-47518. That is, a medium of pH 5.0 consisting of 20 g of glucose, 5 g of yeast extract and 1,000 ml of purified water was inoculated with the agaricus mushroom mycelium cultured in an agar medium,
The cells were precultured by standing still at ℃ for 3 weeks. 500 ml of this preculture
, Glucose 50 g, yeast powder 10 g, antifoaming agent 20 pp
3,000 ml of a main culture medium having a pH of 5.0 and 1,000 ml of purified water was inoculated and cultured under the conditions of a culture temperature of 30 ° C., an aeration rate of 0.5 VVM, and a stirring speed of 200 to 300 rpm. After culturing for 10 days, the mycelium was separated and collected by filtration. Purified water (3,000 ml) was added to this mycelium, and the mixture was heated and extracted at 50 ° C. for 1 hour. After cooling 10,000
Centrifugation was performed at 0 rpm for 30 minutes, and the supernatant was used as Agaricus edulis mycelium extract 3 (Production Example 3).

[Production Example 4] Agaricus mycelia mycelium extract 4 Agaricus mushroom mycelium extract 3 was concentrated under reduced pressure by a rotary evaporator to 1,000 ml. To this, 1,000 ml of ethanol was added, and the mixture was allowed to stand overnight at 4 ° C. to produce a precipitate. Then, the precipitate was collected, washed with acetone and ether and then dried to obtain a pale grayish white powder. Further, this was dissolved in 500 ml of purified water, dialyzed against 5,000 ml of purified water with a dialysis tube, the resulting precipitate was removed by centrifugation, and the supernatant was freeze-dried to obtain a white powder. It was designated as Agaricus edulis mycelium extract 4 (Production Example 4).

[Production Example 5] Agaricus mycelia mycelium culture filtrate 1 At the time of preparation of Production Example 1, the culture liquid after removing the mycelia from the main culture liquid of the Agaricus mycelia mycelium was used as the agaricus mushroom mycelium culture filtrate 1 ( It was referred to as Production Example 5).

[Production Example 6] Fraction 1 of Agaricus mycelium mycelium culture filtrate 1 Agaricus mushroom mycelium culture filtrate 1 was concentrated to 1/6 volume with a rotary evaporator, and an equal volume of ethanol was added thereto at 4 ° C. The mixture was left to stand overnight for a day to form a precipitate.
The precipitate was recovered by centrifugation, washed with acetone and ether, and then dried to obtain a light brown powder, which was used as Agaricus mycelia mycelium culture filtrate fraction 1 (Production Example 6).

[Production Example 7] Agaricus mycelia mycelium culture filtrate 2 At the time of preparation of Production Example 3, the culture liquid after removing mycelia from the main culture liquid of Agaricus mycelia mycelia was used as the agarix mushroom mycelium culture filtrate 2 (Production Example 7).

[Production Example 8] Fraction 2 of Agaricus mycelia mycelium culture filtrate 2 Agaricus mushroom mycelium culture filtrate 2 was concentrated to 1/10 volume with a rotary evaporator, an equal volume of ethanol was added, and the mixture was added at 4 ° C for 1 day / night. The mixture was allowed to stand at, and a precipitate was generated. The precipitate was collected by centrifugation, washed with acetone and ether, and then dried to obtain a light brown powder. This is purified water 30
Dissolve in 0 ml and use dialysis tube to obtain purified water 3,000 m
After dialysis against 1 and removing the resulting precipitate by centrifugation, the supernatant was freeze-dried to obtain a white powder, which was referred to as Agaricus mycelium mycelium culture filtrate fraction 2 (Production Example 8).

Next, the formulations of the examples of the present invention prepared by using the above-mentioned production examples will be shown.

[Example 1] Hair lotion (1) Ethanol 56.0 (wt%) (2) Vitamin B ₆ 0.1 (3) Agaricus mycelium extract 1 (Production Example 1) 2.0 (4) ) 1,3-Butylene glycol 2.5 (5) Perfume 0.1 (6) Purified water 39.3 Manufacturing method: (5) is dissolved in (1), and (2) to (4) are sequentially mixed with (6). Add to, mix, and dissolve uniformly.

[Example 2] Hair nourishing agent (1) Ethanol 60.0 (wt%) (2) Tocopherol acetate 0.5 (3) Agaricus mycelia extract 2 (Production Example 2) 1.0 (4) Agaricus Mushroom mycelium extract 3 (Production Example 3) 2.5 (5) Propylene glycol 2.0 (6) Perfume 0.1 (7) Purified water 33.9 Production method: (6) is dissolved in (1), Add (2) to (5) to (7) in sequence and mix to dissolve uniformly.

[Example 3] Hair foam (stock solution formulation) (1) Methyl cellulose 2.50 (wt%) (2) Polyoxyethylene (50 E.O.) hydrogenated castor oil 1.00 (3) Silicone oil 5. 00 (4) Dipropylene glycol 7.00 (5) Ethanol 15.00 (6) Agaricus mycelium mycelium extract 4 (Production Example 4) 0.20 (7) Methyl paraoxybenzoate 0.15 (8) Perfume 0 10 (9) Purified water 69.05 (filling prescription) Undiluted solution 90.0 Liquefied petroleum gas 10.0 Process: Add (3) to the melts of (2) and (4) and homogenize with a homomixer. To do. This is added to the solution of (1), (5) to (9) and mixed to make uniform. Filling is carried out by filling the can with the stock solution, and after installing the valve, filling with liquefied petroleum gas.

[Example 4] Hair gel (1) Carboxyvinyl polymer 0.50 (wt%) (2) Agaricus mycelia mycelium culture filtrate 1 (Production Example 5) 1.50 (3) Agaricus mushroom mycelium culture filtrate Fraction 2 (Production Example 8) 0.20 (4) Glycerin 2.00 (5) Sodium hydroxide 0.05 (6) Ethanol 20.00 (7) Polyoxyethylene (20E.O.) Stearyl 0.20 Ether (8) Methyl paraoxybenzoate 0.10 (9) Perfume 0.10 (10) Purified water 75.35 Manufacturing method: (1) is dispersed in a part of (4) and (10). to this,
(2), (3) and (6) to (9) are dissolved in the rest of (10), added and mixed, and (5) is added to increase the viscosity.

Example 5 Set Lotion (1) Polyvinylpyrrolidone / vinyl acetate copolymer 5.00 (% by weight) (2) Methyl paraoxybenzoate 0.10 (3) Perfume 0.10 (4) Ethanol 30 0.005 (5) Polyoxyethylene / polyoxypropylene modified 0.50 Dimethylpolysiloxane (6) Glycerin 2.00 (7) Agaricus mycelia mycelium culture filtrate fraction 1 (Production Example 6) 0.25 (8) Purified water 62.05 Manufacturing method: (1) to (3) are added to (4) and uniformly dissolved. To this, the water phase components (5) to (8) previously dissolved are added and uniformly dissolved.

Example 6 Hair Treatment (1) Liquid Paraffin 15.00 (wt%) (2) Vaseline 15.00 (3) Beeswax 2.00 (4) Polyoxyethylene (50 E.O.) Cured Castor Oil 3.00 (5) Glycerin 5.00 (6) Carboxyvinyl polymer 0.05 (7) Xanthan gum 0.05 (8) Disodium ethylenediaminetetraacetate 0.10 (9) Purified water 59.48 (10) Water Sodium oxide 0.02 (11) Agaricus mycelia mycelium extract 1 (Production example 1) 0.10 (12) Agaricus mycelia mycelium culture filtrate 2 (Production example 7) 0.10 (13) Agaricus mycelia mycelium culture filtrate Fraction 2 (Production Example 8) 0.05 (14) Perfume 0.05 Production method: The oil phase components (1) to (3) are dissolved by heating to 80 ° C. On the other hand, the water phase components (4) to (9) are mixed, heated and dissolved,
℃. The oil phase is added to this with stirring, and the mixture is uniformly emulsified with a homogenizer. After cooling, (10) was added at 30 ° C to increase the viscosity, and then mixed and dissolved in advance (1
Add 1)-(13) and (14) and mix.

Example 7 Hair Shampoo (1) Polyoxyethylene (3E.O.) Lauryl Sulfate 30.00 (wt%) Ester Sodium Salt (30 wt% Aqueous Solution) (2) Lauryl Sulfate Ester Sodium Salt 10. 00 (30% by weight aqueous solution) (3) Coconut oil fatty acid diethanolamide 4.00 (4) Glycerin 1.00 (5) Sodium dehydroacetate 0.20 (6) Ethylenediaminetetraacetic acid disodium 0.05 (7) Perfume 0 10 (8) Purified water 54.15 (9) Agaricus mycelia mycelium extract 2 (Production example 2) 0.25 (10) Agaricus mycelia mycelium culture filtrate fraction 1 (Production example 6) 0.25 Production method : (8) is heated to 70 ° C., (1) to (6) are added, uniformly dissolved and cooled, and (7), (9) and (10) are added and mixed at 30 ° C., Dissolve uniformly.

[Example 8] Hair rinse (1) Silicone oil 3,000 (% by weight) (2) Liquid paraffin 1.000 (3) Cetanol 1.500 (4) Stearyl alcohol 1.000 (5) Stearyl chloride Trimethylammonium 0.700 (6) Glycerin 3.000 (7) Methyl paraoxybenzoate 0.200 (8) Green No. 3 0.002 (9) Purified water 87.423 (10) Perfume 0.150 (11) Agaricus Mushroom mycelium extract 3 (Production example 3) 1.000 (12) Agaricus mycelia mycelium extract 4 (Production example 4) 0.025 (13) Agaricus mycelia mycelium culture filtrate 1 (Production example 5) 1.000 Production method: The aqueous phase components (5) to (9) are mixed, dissolved and heated to 70 ° C. On the other hand, the oil phase components (1) to (4) are mixed and heated to 70 ° C. The oil phase is added to the aqueous phase, and the mixture is emulsified with a homomixer. After cooling, (10) and (11) to (13) previously mixed and dissolved are added and mixed at 40 ° C.

Of the above-mentioned Examples, Example 1, Example 2 and Example 6 were subjected to a usage test by a panelist having thinning hair and hair loss symptoms. As the panelists, men and women in their 20s to 60s having remarkable thinning hair and hair loss symptoms were used as one group of 20 people. In each Example, the extract of Agaricus mycelia mycelium, the culture filtrate of Agaricus mycelia mycelium and fractions thereof were replaced with purified water to form Comparative Example 1, Comparative Example 2 and Comparative Example 6, respectively. Each group was blindly used twice a day for 6 months. The state of the hair before and after the start of the use test was photographed and evaluated according to the criteria shown in Table 2 and scored, and the average value of 20 persons was calculated and shown in Table 3.

[Table 2]

[0045]

[Table 3] As shown in Table 3, in each of the groups used in the examples of the present invention, significant promotion of hair growth and hair growth was observed. In particular, in the group of Example 2 containing 1.0% by weight and 2.5% by weight of Agaricus edodes mycelium extracts 2 and 3 of Production Example 2 and Production Example 3, respectively, remarkable improvement in thinning hair and hair loss was observed. Was given. On the other hand, almost no change was observed in the hair condition in each group used in Comparative Examples.

Next, with respect to Examples 6 to 8, the effect of improving dandruff and itch was evaluated. Similarly to the above, in each example, the extract of Agaricus edulis mycelium, the culture filtrate of Agaricus edulis mycelium and the fraction thereof was replaced with purified water as Comparative Examples. One group of 20 male and female panelists in their 20s to 50s having marked dandruff and itching was used, and each group was allowed to use the Examples and Comparative Examples blindly once a day for 1 week. About the symptoms of dandruff and itch,
The evaluation was made in three stages of “improvement”, “slight improvement” and “no change”, and the number of panelists who obtained each evaluation is shown in Table 4.

[0047]

[Table 4] As is clear from Table 4, in the use groups of Examples of the present invention,
70% or more of the dandruff and 65% or more of the itch experienced a clear improvement, and none of the panelists had any improvement in their symptoms. On the other hand, no panelists showed a clear improvement in the groups used in each comparative example, and 60% of dandruff and 50% or more of itchy panelists did not show a tendency of improvement of symptoms.

In Examples 1 to 8 of the present invention, no change in state was observed when stored at 25 ° C. for 6 months, and good stability was exhibited. Also, male panelists 3
No skin irritation was observed in a 48-hour occlusion patch test by 0 persons, and no irritation or discomfort during use was observed in a use test by 35 male and female panelists.

[0049]

As described above in detail, according to the present invention, hair follicles are activated to effectively promote hair growth and hair growth, and further improve the condition of the scalp to effectively prevent dandruff and itch. It was possible to obtain an improved hair nourishing agent and hair cosmetic composition.

─────────────────────────────────────────────────── ─── Continuation of the front page (56) References JP-A-11-79949 (JP, A) JP-A-5-345726 (JP, A) JP-A-7-316026 (JP, A) (58) Field (Int.Cl. ⁷ , DB name) A61K ^7/ 00-7/50 JISST file (JOIS) CA (STN)

Claims (2)

(57) [Claims] 1. Agaricus blazei Murill
A hair nourishing agent and a hair cosmetic composition comprising a mycelium extract. 2. Agaricus blazei Murill
A hair nourishing agent and a cosmetic for hair, comprising a mycelium culture filtrate.