JP3524282B2 - Hair growth promoter for hair growth - Google Patents

Description

Description: TECHNICAL FIELD The present invention relates to an excellent hair growth promotion
On use hair tonic. [0002] Alopecia is thought to be caused by various factors such as insufficiency in blood circulation, decreased hair matrix activity, and imbalance of sex hormones. At present, it is not fully understood. Conventionally, hair restorations containing various agents have been used for prevention and treatment of hair loss, but no agent that is sufficiently effective to suppress hair loss and promote hair growth has yet to be found. On the other hand, Aconitum buttercups (Ran
unculaceae (Aconit)
Um species) is an important herbal medicine that has been described in the ancient Chinese herb sutra in ancient China and is widely used in various Chinese herbal prescriptions in Chinese herbal medicine. JP-A-3-271213 discloses a hair-growth and hair-growth agent characterized by containing an extract of roots, stems or leaves of aconite, and JP-A-5-2550.
No. 99, hair growth characterized by containing non-aconitine type alkaloid obtained from the roots of Aconitum,
Hair restorers have been proposed. However, it was found that a detailed study of these hair growth and hair growth effects was not always sufficient. A hair restoration comprising a combination of an extract of roots, stems or leaves of aconite and a drug has also been studied (Japanese Patent Application Laid-Open No. Hei 5 (1993) -107).
-255047). It can easily be seen from the comparison with the present invention described below that the effect of the present invention due to the extract of root, stem or leaf of aconite is not always sufficient. [0004] In view of the above circumstances, the present inventors have conducted intensive studies and as a result obtained by treating the roots, stems or leaves of aconite with ethyl acetate or n-hexane . by Ruru be blended extract, it found that shows the superior effect on hair growth promoting, and have completed the present invention. [0005] The present invention provides a hair growth promoting method characterized by incorporating an extract obtained by treating roots, stems or leaves of aconite with ethyl acetate or n-hexane.
Use hair tonic. Hereinafter, embodiments of the present invention will be described in detail. Aconite, a raw herbal medicine used for the hair growth promoter for promoting hair growth of the present invention, is a Ranunculus family (Ranunculac).
aae) Aconitum spec.
iss), and the place of production is not limited. In general,
Aconitum plants contain highly toxic aconitine-based alkaloids, and their use requires caution. Therefore, it is preferable to use those that have been detoxified by heat treatment or the like. [0007] As a base plant of aconite, for example,
Aconitou (Aconitu), a cultivar from Sichuan, China
m carmichaeli Debx), wild species (Aconitum kusnezoffiii) from various parts of China
Reichb. ) And other honey beetles from Korea (A
conitum triphyllum Naka
i), Aconitum core
anum Rap. ), Japanese Octopus beetle (Ac
onitum japonicum Thumb. ),
Honey beet (Aconitum chinense)
Paxt. ) And the like. In particular, Chinese beetle from Sichuan Province, China is preferable in terms of quality stability and supply with a certain cultivation method. [0008] As a method of detoxification, the tuber root is placed in an autoclave at 100 to 130 ° C under a pressure of 1.0 to 1.5 kg.
The method of preparing by pressurizing and heating at 20 / cm ² for 20 to 40 minutes is particularly preferable because a simple, effective and safe crude drug can be stably produced (JP-B-37-18498). As an example prepared by this method, there is “Processed Bushi Powder” which is a non-Japanese Pharmacopoeia standard product. In addition, as a method mainly performed in China, the tubers washed with water are immersed in bitter solution for several days and dried, or are further steamed, a method of preparing by drying after repeated treatments such as boiling, or There is a method in which the tuberous root is washed with water, vertically divided, buried in ash at the time of freshness, dried by heating and pressurizing, and the like. As an example prepared by this method, a commercially available aconite from Sichuan, China, which is sufficiently detoxified with a certain quality, is preferable. [0009] The extract of aconite in the present invention comprises ethyl acetate
Refers to the component that has been converted from aconite to ethyl acetate or n-hexane when extracted with a solvent of n-hexane or n-hexane.This extract must be used in the form of a dried product after removing ethyl acetate or n-hexane. Acetic acid
It can also be used in the state of being dissolved in ethyl or n-hexane or another solvent. In the latter case, ethyl acetate
Or n-hexane constitutes a part of the base in the hair growth promoting hair growth material of the present invention. In the extraction, the aconite can be used as it is, or can be used after being crushed or pulverized to improve the contact with the solvent. The ratio between the aconite and the solvent is not particularly limited, but from the viewpoint of extraction efficiency and convenience, 100 to 5000 ml per 100 g of aconite.
It is preferred to use a solvent of It is convenient to set the extraction temperature within the range of the boiling point of the solvent at room temperature and normal pressure, and the extraction time varies depending on the extraction temperature and the presence or absence of stirring.
Preferably it is in the week range. [0011] The hair growth promoting hair tonic of the present invention in accordance with a conventional method, hair tonic, hair lotion, hair cream, shampoo, rinse, hair form, it is possible to on the dosage form such as hair gel. These hair growth promotion
Extract of the present invention to be contained in use hair tonic fees, in terms of dry residue, 0.0001 wt%, preferably 0.0
It may be 005 to 5% by weight. [0012] The hair growth promoter for promoting hair growth of the present invention comprises a pigment,
Flavoring agents, bactericides, preservatives, keratolytic agents, anti-inflammatory agents, anti-androgens, hair restoration agents, antioxidants, fresheners, other crude drug extracts and vitamins as appropriate within the scope of achieving the object of the present invention. Can be blended. [0013] [Embodiment] Hereinafter will be described in detail the present invention based on production examples, examples and comparative examples. The mouse hair growth promoting effect test method, the human hair growth promotion effect test method, and the practical test methods described in the Examples are shown below. [0014] (1) mouse hair growth promoting effect test method C3H mice (male, 8 weeks old) were shaved by clippers and a shaver hair on the back center of the, 2 times a day the sample to the shaved site, per animal 0.2 ml was applied. A group of 10 animals was used for one sample. On the 15th day after the start of the experiment, a photograph of the back of the mouse was taken, the shaved area and the hair growth area were calculated, the hair growth rate (%) was determined, and the hair growth promoting effect was determined by averaging 10 cases. [0015] (2) shaved in a circular shape with a diameter of 1cm in human hair hair growth promoting effect test method male pattern baldness patient left and right two places the hair of the position of the top 5cm of the ear of the head of the test's 10 people, which is a About 3 ml of the sample of the example or the comparative example was applied to the hairy test site twice a day on the left side every morning and evening, and compared with the untreated right side. The effect was evaluated on the 28th day after the test started.
Twenty hairs on each of the left and right test sites were shaved, and the average value (B) of the lengths of the twenty hairs on the left (coated with the example or the comparative example) was calculated for the twenty hairs on the right (untreated). The value divided by the average value (A) of the length was obtained and evaluated. The judgment results were shown as the average value of (B) / (A) of 10 test subjects. [0016] (3) practical test method male pattern baldness every morning and evening twice in the head of the test's 20 people that is a patient, to evaluate the effect of after the application of the continuous 6-month sample. Test results show that for each item of hair growth effect and hair loss prevention effect, "grown hair or increased hair growth",
The number of respondents who answered that "hair loss was reduced" was indicated. [0017] Production Example 1 China four provinces Ministry of producing color aconite crushed dried tuberous root in an autoclave 100~130 ℃, 1~1.5kg / c
m ² and pressurized and heated for 40 minutes. 100 g of the processed beetle was soaked in 1500 ml of ethyl acetate at 65 to 75 ° C. for 3 hours, the supernatant was collected by filtration, concentrated at 70 ° C. under reduced pressure for 6 hours, and the solvent was distilled off. 0.41 g of the product was obtained. [0018] Production Example 2 China four provinces Ministry of producing color aconite crushed dried tuberous root in an autoclave 100~130 ℃, 1~1.5kg / c
Pressurization and heat treatment for 40 minutes at m ² . 100 g of the processed aconite was taken up in 65 ml of 1500 ml of n-hexane.
After soaking at 75 ° C. for 3 hours, the supernatant was collected by filtration, concentrated under reduced pressure at 70 ° C. for 6 hours, and the solvent was distilled off.
g were obtained. [0019] Production Example 3 China four provinces Ministry of producing color aconite crushed dried tuberous root in an autoclave 100~130 ℃, 1~1.5kg / c
Pressurization and heat treatment for 40 minutes at m ² . 100 g of processed beetle was added to 1500 ml of ethanol
Immersed at ~ 75 ° C for 3 hours, and the supernatant was collected by filtration.
After concentrating under reduced pressure for 6 hours and evaporating the solvent, the extract was subjected to 5.4 extraction.
6 g were obtained. [0020] Production Example 4 China four provinces Ministry of producing color aconite crushed dried tuberous root in an autoclave 100~130 ℃, 1~1.5kg / c
Pressurization and heat treatment for 40 minutes at m ² . 100 g of the processed beetle was immersed in 1500 ml of 70 v / v% ethanol at 65 to 75 ° C. for 3 hours, the supernatant was collected by filtration, concentrated at 70 ° C. under reduced pressure for 6 hours, and the solvent was distilled off. rear,
10.01 g of the extract was obtained. [0021] Example 1, 2, Comparative Examples 1 and 2 (hair growth promoting
In the component composition according to use hair tonic) below, formulated to each hair growth promoting color aconite extract prepared in Preparation Example 1-5
A hair tonic was prepared and the above test was performed. [0022] (1) Composition Ingredients Amounts remaining [0023] to (wt%) Ethanol 60.0 Aconitum extract 0.5 Propylene glycol 1.0 Perfume 0.1 Purified water total amount 100.0 (2 ) Preparation method Extracts and flavors of Acacia catechu were dissolved in ethanol, and propylene glycol was dissolved in purified water, and the solutions were uniformly mixed while stirring to prepare hair tonics for promoting hair growth . [0024] (3) Characteristics Table 1 the results of embodying test of each hair growth promoting hair tonic, shown in Table 2. [ Table 1] [ Table 2] [0027] These results, Example 1, 2 of the hair growth promoting hair tonic formulated with extract of the present invention, ethanol aconite, hair growth in Comparative Examples 1 and 2 was blended with 70% ethanol extract indicating that it is very good in terms of hair growth promoting effect than for promoting hair tonic. [0028] As above described, according to the present invention, the present invention exhibits a remarkable hair growth promoting effect, it is clear that providing a hair tonic for hair growth promoting are excellent.

Claims (1)

(57) [Claims] [Claim 1] The root, stem or leaf of Aconitum is washed with ethyl acetate.
Alternatively , a hair growth-promoting hair growth promoting composition comprising an extract obtained by treating with n-hexane .