JP3537671B2 - Lipolysis accelerator and skin cosmetic for slimming - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

[0001] The present invention relates to the improvement of obesity constitution by promoting the reduction of systemic or local adipose tissue, or the promotion of lipolysis which is effective in suppressing or preventing obesity by preventing the increase of such tissue. And a skin cosmetic for topical slimming containing the lipolysis promoter.

[0002]

2. Description of the Related Art Fat in the body is produced by accumulating excess intake energy with respect to consumed energy as neutral fat in white fat cells. Obesity, which has a large accumulation as body fat, is not only cosmetically unfavorable, but also causes various diseases such as arteriosclerosis, and is also unfavorable in health. In recent years, obesity due to overeating, lack of exercise, stress and the like has been increasing. Therefore, reduction of the subcutaneous fat or prevention of accumulation is an important problem. For this reason, there have been various approaches using oral drugs such as appetite suppressants, dietary restrictions, exercise, and the like. It was not served.

[0003]

Therefore, the present invention is effective for improving obesity constitution by promoting the reduction of systemic or local adipose tissue, or for suppressing or preventing obesity by preventing the increase of the tissue. It is an object of the present invention to provide a lipolysis accelerator and a skin cosmetic for slimming.

[0004]

In order to achieve the above object, it is difficult for the present inventors to eliminate fat cells once accumulated. We thought that it was necessary to break up oil droplets in fat cells, and studied intensively. Oil droplets are broken down by enzymes (phospholipase C) in the same way as proteins.
However, the oil droplets are covered by a membrane of phospholipid that repels water, and enzymes in a mass of water, a parcel around the oil droplets, cannot be accessed. On the other hand, the lipolytic hormone is secreted by activating the sympathetic nerve by exercising, and the decomposition of fat is promoted. At this time, the hormone removes the phospholipid film and promotes lipolysis by increasing the affinity between the enzyme and the oil droplets. We thought that it was necessary to find a substance that had the function of increasing the affinity between oil droplets and enzymes, as well as this hormone. Therefore, the present inventors studied such a substance that acts on fat cells having such an action and promotes lipolysis.As a result, extracts extracted from genoshoko, peonies, assembly, and thyme have the above-described actions, The present inventors have found that the present invention promotes the decomposition of fat accumulated in adipose tissue and is effective in suppressing obesity or improving obesity, and completed the present invention.

That is, the present invention relates to
Lipolysis accelerators containing sulfur. Also, the present invention
In topical slimming skin cosmetic which is characterized by containing the cranesbill equi scan or Ranaru lipolysis promoter.

[0006]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, methods for producing various plant extracts used in the present invention will be described, but the present invention is not limited thereto. The various plant extracts referred to in the present invention are, in addition to the liquid extract obtained by the following method, a liquid extract obtained by drying or concentrating the liquid extract by a normal drying method such as reduced-pressure drying or freeze-drying, a concentration method, or the like. There may be. Further, if necessary, it may be used after purification treatment such as deodorization and decolorization within a range that does not affect its efficacy. Formulations may be used.

[0007] The Geno ginger extract used in the present invention is Geranium thunbergii Siebold
et Zuccarini) extracted from the aerial part with a 1,3-butylene glycol solution or an ethanol solution.

The peony extract is a peony (Paeoni)
a lactiflora Pallas) or other closely related plants (Paeo
niaceae, from the fine roots of the root, ethanol, 1,
It is an extract extracted with 3-butylene glycol, purified water or a mixture thereof.

[0009] The assembly extract is obtained from the assembly (Swertia ja
ponica Makino), which can be obtained by extracting with ethanol, anhydrous ethanol, water or a mixture thereof from the whole flowering plant. Assembly is used as a bitter stomach medicine for indigestion and anorexia. In addition, it has been recognized that it has a peripheral vasodilatory effect, a skin temperature increasing effect, and an effect of promoting hair growth during anagen hair, and is used in basic cosmetics and hair cosmetics.

The thyme extract is thyme (Thymus serpyll)
um L.), which is extracted with water, ethanol, 1,3-butylene glycol, or a mixture thereof from above. Contains essential oils, tannins, flavonoids, and triterpenoids. It has astringent, cleansing and moisturizing effects.

The above various plant extracts can be used alone or in combination of two or more.
Although it varies depending on the form of the skin cosmetic for topical slimming, for example, it is preferable to add 0.001 to 20% by weight in the composition. If the amount is less than 0.001% by weight, the effect of the present invention may not be exhibited, and if the amount exceeds 20% by weight, the effect corresponding to the amount may not be obtained.

The skin cosmetic for topical slimming of the present invention includes oils, pigments, surfactants, moisturizers, ultraviolet absorbers, which are used in ordinary skin cosmetics in addition to the above plant extracts.
In addition to anti-inflammatory agents, bactericides, preservatives, pigments, butpamine,
Β-adrenergic stimulants such as isoproterenol, α2 adrenergic inhibitors such as yohimbine and ergotoxin, theophylline, xanthine derivatives such as caffeine, milrinone, bipyridine derivatives such as amrinone, etc.
Known substances having an effect of suppressing or preventing obesity can be further added.

The lipolysis promoter of the present invention can be incorporated into, for example, foods, orally administered drugs, skin cosmetics and the like. The form of the skin cosmetic for topical slimming is not particularly limited, and may be a cream, an emulsion, a gel, a stick, a sheet, a pup, a powder, a liquid, a granule, etc. for application to the skin, bath, and washing. be able to.

[0014]

The present invention will be described below in detail based on examples and comparative examples. The lipolysis promoting effect was evaluated by the following lipolysis test and subcutaneous lipolysis test.

[Lipolysis test method] Rodbell method [Rodbell M. , J. et al. Bio
l. Chem. 239, 375 (1964)], male Wistar rats (body weight 150-200 g).
Free adipocytes were prepared from epididymal adipose tissue using a collagenase solution. The cells were added to a Hanks buffer containing bovine serum albumin and norepinephrine (final concentration: 0.05 μg / ml) adjusted to a test substance concentration of 100 μg / ml, and reacted at 37 ° C. for 1 hour. The released fatty acid was extracted, and the amount of the fatty acid was quantified using a copper reagent and a coloring reagent.

Lipolysis promotion rate (%) = [A / B] × 10
0 A: Fatty acid amount when sample is added B: Fatty acid amount when control is added

[Subcutaneous lipolysis test method] The abdomen of Wistar rats (male, 7 to 9 weeks old) was shaved, and the skin was excised together with the subcutaneous adipose tissue to obtain a diameter 2c.
m Franz type diffusion cell. To the upper cell on the stratum corneum side, 0.5 g of the skin cosmetic for slimming, which is a test substance, is uniformly applied, and the lower cell on the side of the subcutaneous fat tissue is filled with pH 7.2 phosphate buffered saline. After the cell was kept at 37 ° C. and left for 6 hours, a buffer solution was collected from the lower cell, and the amount of glycerol released into the solution was measured by an enzyme method (F-kit; glycerol, manufactured by Boehringer Mannheim). still,
The measurement was performed five times and the average value is shown.

Example 1 , Reference Examples 1 to 3 and Comparative Examples 1 to 5 Table 1 shows the results of the evaluation of various plant extracts and the like by the lipolysis test method. In addition, each plant extract is 50%
The extract was extracted with an aqueous ethanol solution, and the extract obtained by freeze-drying the extract was used.

[0019]

[Table 1]

As shown in Table 1, it was found that the plant extract of Example 1 had a much higher lipolysis promotion rate than the control and a higher lipolysis promotion action. Note that Comparative Example 5 uses caffeine, which is known to have a lipolytic effect, and the lipolysis promoting rate confirms that this test method is correct. In addition, some of the examples had an action equal to or higher than that of caffeine.

Example 2, Reference Examples 4 to 6 , Comparative Example 6 (Gel-like Slimming Skin Cosmetic) As shown in Table 2, components A and B were individually mixed and dissolved according to a conventional method, and component B was converted to component A. And agitated to prepare a gel-like slimming skin cosmetic. Here, the plant extract mixed with the gel-like slimming skin cosmetic was extracted with a 50% aqueous ethanol solution, and the extract was freeze-dried and used.

[0022]

[Table 2]

The gel-like slimming skin cosmetic is
Table 3 shows the results of the evaluation by the above-described subcutaneous lipolysis test method.

[0024]

[Table 3]

Example 3, Reference Examples 7 to 9 and Comparative Example 7 (Lotion-like slimming skin cosmetic) As shown in Table 4, components A and B were separately mixed and dissolved according to a conventional method, and component B was converted to component A. And agitated to prepare a lotion-like slimming skin cosmetic. In addition, the plant extract blended in the lotion-like slimming skin cosmetic is described in Examples.
2. Those used in Reference Examples 4 to 6 were used.

[0026]

[Table 4]

Table 5 shows the results of the above lotion-like slimming skin cosmetics evaluated by the above-described subcutaneous lipolysis test method.
Shown in

[0028]

[Table 5]

As is clear from Tables 3 and 5, Example 2
Nos. 3 to 3 showed that the amount of free glycerol, which is a lipolysis product, in the subcutaneous lipolysis test was remarkably larger than that of the comparative example, and promoted subcutaneous lipolysis.

[0030]

As described above, the present invention provides a lipolysis-promoting agent effective for reducing systemic or local adipose tissue, suppressing or preventing obesity, and improving obesity, and local slimming having the effect. It is clear that skin cosmetics can be provided.

──────────────────────────────────────────────────続 き Continuation of the front page (56) References JP-A-11-269079 (JP, A) JP-A-11-130656 (JP, A) JP-A-2000-63259 (JP, A) JP-A-2000-63260 ( JP, A) JP-A-2000-63227 (JP, A) JP-A-2-243632 (JP, A) JP-A-62-207289 (JP, A) (58) Fields investigated (Int. Cl. ⁷ , DB Name) A61K 7/00-7 / 50,35 / 78

Claims (2)

(57) [Claims] 1. A lipolysis promoter containing cranesbill equi scan. Wherein cranesbill equi scan or Ranaru topical slimming skin cosmetic which is characterized in that it contains a fat decomposition accelerator.