JP3769098B2 - New microorganisms belonging to the genus Bacillus - Google Patents
New microorganisms belonging to the genus Bacillus Download PDFInfo
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- JP3769098B2 JP3769098B2 JP12015797A JP12015797A JP3769098B2 JP 3769098 B2 JP3769098 B2 JP 3769098B2 JP 12015797 A JP12015797 A JP 12015797A JP 12015797 A JP12015797 A JP 12015797A JP 3769098 B2 JP3769098 B2 JP 3769098B2
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- Prior art keywords
- bacillus
- genus bacillus
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- microorganism
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- 244000005700 microbiome Species 0.000 title claims description 28
- 241000193830 Bacillus <bacterium> Species 0.000 title claims description 10
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 6
- 239000002184 metal Substances 0.000 claims description 6
- 229910052751 metal Inorganic materials 0.000 claims description 6
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 4
- 229910052698 phosphorus Inorganic materials 0.000 claims description 4
- 239000011574 phosphorus Substances 0.000 claims description 4
- 239000002351 wastewater Substances 0.000 claims description 4
- 238000004332 deodorization Methods 0.000 claims description 3
- 150000002739 metals Chemical class 0.000 claims description 3
- 229910052757 nitrogen Inorganic materials 0.000 claims description 3
- 239000004912 1,5-cyclooctadiene Substances 0.000 claims 1
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 description 9
- 238000000746 purification Methods 0.000 description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 5
- 239000000843 powder Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 230000001877 deodorizing effect Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 229910001562 pearlite Inorganic materials 0.000 description 3
- 241000220223 Fragaria Species 0.000 description 2
- 239000005909 Kieselgur Substances 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 229910021536 Zeolite Inorganic materials 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 2
- 239000006185 dispersion Substances 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000010457 zeolite Substances 0.000 description 2
- 235000016623 Fragaria vesca Nutrition 0.000 description 1
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 230000000721 bacterilogical effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 239000004745 nonwoven fabric Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000021012 strawberries Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 239000000454 talc Substances 0.000 description 1
- 229910052623 talc Inorganic materials 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 239000002759 woven fabric Substances 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Description
【０００１】
【産業上の利用分野】
本発明は、バチルス（Bacillus) 属に属する新規微生物に関する。
【０００２】
【従来の技術】
排水浄化菌としては、各種のバチルス属微生物が知られている。しかしながら、排水浄化においては、単に浄化に止まらずに、その脱臭や、金属物質の除去も大切な要素である。
【０００３】
しかしながら、現在までに知られているバチルス属微生物では、これらの全ての要素を満足する微生物は発見させれおらず、そのような微生物が強く望まれていた。
【０００４】
【発明が解決しようとする課題】
そこで、本発明は、排水の単なる浄化とともに、その脱臭や金属除去もできる新規な微生物を提供することを目的とするものである。
【０００５】
【課題を解決するための手段】
本発明らは、上記のような要素を全て満足することのできる有用な新菌株を求めて鋭意探索したところ、浄化、脱臭、金属除去の全ての機能を有する一菌株を土壌から分離することに成功した。この一菌株の菌学的性質の試験および分類は、下記の文献に従い行なった。
【０００６】
▲１▼バージェイズ マニュアル オブ デターミネティブ バクテリオロジー 第９版（1994年）（Bergey's Manual of Determinaitive Bacteriology 8th Edition）
▲２▼バージェイズ マニュアル オブ システマティック バクテリオロジー 第２版（1986年）（Bergey's Manual of Systematic Bacteriology 2nd Edition）
▲３▼ザ ジーナス バチルス（1973年）（U.S. Department of Agriculture)
【０００７】
その結果、その微生物は、耐熱性の胞子を形成するグラム陽性桿菌であることからバチルスであると同定されたが、菌体成分の分析結果が、上記の文献に記載されているいずれの菌種とも一致せず、バチルスに属する新菌であることが確認された。 当該微生物は、Bacillus sp. CCC-a（寄託番号：ＦＥＲＭ Ｐ−１６１７５）として工業技術院生命工学研究所微生物寄託センターに寄託されている。
【０００８】
以下、本発明のバチルスに属する新規微生物の菌学的性質を説明する。
【０００９】
本発明の新規微生物は、一般栄養培地であるならいかなる培地でも良好に成育する。
【００１１】
培養は、好ましくは５〜３０℃程度で良好に増殖する。
【００１２】
本発明の新規微生物は、水や液体培地に分散させ分散液の状態で各種用途に用いることができるが、好ましくは微生物群を担体に固定し、取扱を容易にした担体固定化微生物群として用いる。
【００１３】
微生物群を固定する担体としては、微生物の保持容量が大きく、また微生物の活性化が容易であることがこのましい。
【００１４】
担体としては、岩石（例えば、真珠岩、珪藻土）またはその粉砕物、砂利、砂、プラスチックス、セラミックス（例えば、アルミナ、シリカ、天然ゼオライト、合成ゼオライト）、タルク等も好ましく、特に、多孔質セラミックス、多孔質プラスチックスのような連続通気孔を有する多孔質材料が好ましい。例えば、真珠岩の粉砕物を高温高圧で保持し熱処理した後、急激に減圧して多孔質にした微細粉（商品名：パーライト）は、菌類を着生させる性質があるため、好適である。このような多孔質材料において、通気孔の径は、２〜１０μｍ程度であることが好ましい。また、このような担体の性状としては、塊状物、粒体、粉体、微粉末またはいた状物、針状物等いかなるものでもよいが、好ましくは平均粒径が２ｍｍ以下、特に５０μｍ〜１ｍｍ程度の粉粒体がよい。なお、このような担体固定化微生物群は、必要に応じて、布やネット等の通水性や通気性のよい容器に収納して使用してもよい。
【００１５】
上記担体としては、これらの他、リボン状やシート状の織布や不織布を用いることができる。
【００１６】
このような担体に微生物群を担持させるには、担体と本発明の微生物群分散液とを混合した後、乾燥すればよいが、担体で直接微生物群を培養してもよい。
【００１７】
担体の微生物群の担持量は、担持させるときの条件によっても異なるが、好ましくは、５個〜２００億個／ｃｍ3 、好ましくは１０個〜１００億個／ｃｍ3 である。
【００１８】
本発明の担体固定化微生物群は、排水の浄化に有効である。また、本発明の新規微生物は、養豚所、公衆便所等に用いて、脱臭効果が確認された。さらに、本発明の新規微生物においては、リン等の金属の回収機能が確認された。さらにまた、土壌の改質にもちいても効果が確認された。
【００１９】
なお、本発明の新規微生物は、マウスによる経口投与試験によりその安全性が確認された。
【００２０】
【実施例】
次に、本発明の実施例について説明する。
まず、次のような組成の培地を作成した。
【００２１】
培地
ペプトン ５ｇ
肉エキス ５ｇ
ＮａＣｌ ２．５ｇ
水 ５００ｍｌ（ｐＨ７．０）
以上の組成の培地を、１０００ｍｌ容器にいれ、これにBacillus sp. CCC-aを接種し、よく撹拌した後、静置して、３０℃で３日間培養した。
【００２２】
得られた培養液を遠心分離機かけ、菌体を分離し、これをよく洗浄し、担体に付着させてパウダーとした。担体としては珪藻土焼いて、粉砕したものを用いた。
【００２３】
このパウダー状のBacillus sp. CCC-aを、計量対象へ接種してのＣＯＤ、全窒素量および全リン量の経時変化を計量した。試験方法は、ＣＯＤはＪＩＳ ０１０２−１７、全窒素量はＪＩＳ ０１０２−４５．２、そして全リン量ＪＩＳ ０１０２−４６．２に従っておこなった。計量対象としては、海水での牛乳１００倍液に、１リットル当たり０．２ｇの澱粉を加えたものを用いた。そのままのものを比較例とし、これにBacillus sp. CCC-aを接種したものを実施例とした。試験は、２０℃に保って行なった。また、各量の測定にあたっては、計量対象液を２時間静置し、その上澄み液を試験液とした。
【００２４】
試験の結果を表１に示した。なお、試験は、それぞれサンプルを２つずつ作製し、その平均を測定値とした。
【００２５】
【表１】
表１から分かるように、本発明の新規微生物であるBacillus sp. CCC-aは、水の浄化機能を有し、金属を除去する作用があることが判明した。さらに、１本のままの腐った鰯にBacillus sp. CCC-aのパウダーを振りかけ２時間経過後にその臭いをかいだところ、明らかに臭気が減少していた。また、新鮮な鰯にBacillus sp. CCC-aを振りかけ、２５℃で保管したところ４８時間を経過しても腐臭はしなかった。一方、Bacillus sp. CCC-aを振りかけずにそのままの状態で２５℃で保管した鰯は、２４時間経過でかなり臭いがきつくなった。
【００２７】
このように、本発明のBacillus sp. CCC-aは、脱臭作用および鮮度保存作用も備えている。[0001]
[Industrial application fields]
The present invention relates to a novel microorganism belonging to the genus Bacillus.
[0002]
[Prior art]
Various bacteria of the genus Bacillus are known as drainage purification bacteria. However, in wastewater purification, not only purification but also deodorization and removal of metal substances are important elements.
[0003]
However, the Bacillus microorganisms known to date have not found any microorganisms that satisfy all these factors, and such microorganisms have been strongly desired.
[0004]
[Problems to be solved by the invention]
Accordingly, an object of the present invention is to provide a novel microorganism capable of deodorizing and removing metal as well as simply purifying waste water.
[0005]
[Means for Solving the Problems]
The present inventors have eagerly searched for a useful new strain that can satisfy all of the above-mentioned elements, and have isolated one strain having all functions of purification, deodorization, and metal removal from the soil. Successful. The test and classification of the bacteriological properties of this strain were carried out according to the following literature.
[0006]
(1) Bargey's Manual of Determinaitive Bacteriology 8th Edition (1994)
▲ 2 Bergey's Manual of Systematic Bacteriology 2nd Edition (1986)
(3) The Genus Bacillus (1973) (US Department of Agriculture)
[0007]
As a result, the microorganism was identified as a Bacillus because it is a Gram-positive bacillus that forms heat-resistant spores, but the analysis result of the bacterial cell component is any bacterial species described in the above literature It was confirmed that it was a new bacterium belonging to Bacillus. The microorganism is deposited at the Microorganism Deposit Center of the Institute of Biotechnology, National Institute of Advanced Industrial Science and Technology as Bacillus sp. CCC-a (deposit number: FERM P-16175).
[0008]
Hereinafter, the mycological properties of the novel microorganism belonging to the Bacillus of the present invention will be described.
[0009]
The novel microorganism of the present invention grows well in any medium that is a general nutrient medium.
[0011]
The culture preferably grows well at about 5 to 30 ° C.
[0012]
The novel microorganism of the present invention can be dispersed in water or a liquid medium and used for various purposes in the form of a dispersion. Preferably, the microorganism group is immobilized on a carrier and used as a carrier-immobilized microorganism group that facilitates handling. .
[0013]
As a carrier for immobilizing a microorganism group, it is preferable that the holding capacity of microorganisms is large and the activation of microorganisms is easy.
[0014]
As the carrier, rocks (for example, pearlite, diatomaceous earth) or a pulverized product thereof, gravel, sand, plastics, ceramics (for example, alumina, silica, natural zeolite, synthetic zeolite), talc and the like are also preferable. A porous material having continuous ventilation holes such as porous plastics is preferred. For example, a fine powder (trade name: pearlite) obtained by holding a pearlite pulverized product at a high temperature and high pressure and heat-treating and then rapidly reducing the pressure to make it porous is preferable because it has the property of causing fungi to grow. In such a porous material, the diameter of the vent is preferably about 2 to 10 μm. The carrier may have any properties such as a lump, granule, powder, fine powder or crumb, needle, etc., but preferably has an average particle size of 2 mm or less, particularly 50 μm to 1 mm. Good granular material. Such a carrier-immobilized microorganism group may be housed and used in a container having good water permeability or air permeability such as cloth or net as necessary.
[0015]
In addition to these, a ribbon-like or sheet-like woven fabric or non-woven fabric can be used as the carrier.
[0016]
In order to support the microorganism group on such a carrier, the carrier and the microorganism group dispersion of the present invention are mixed and then dried, but the microorganism group may be directly cultured on the carrier.
[0017]
Loading amount of microorganisms in the carrier varies depending on the conditions at the time of loading, preferably, 5 to 200 billion pieces / cm 3, preferably 10 to 100 billion pieces / cm 3.
[0018]
The carrier-immobilized microorganism group of the present invention is effective for purification of waste water. Moreover, the deodorizing effect was confirmed by using the novel microorganism of the present invention in pig farms, public toilets, and the like. Furthermore, in the novel microorganism of the present invention, the function of recovering metals such as phosphorus was confirmed. Furthermore, the effect was confirmed also in soil improvement.
[0019]
The safety of the novel microorganism of the present invention was confirmed by an oral administration test using mice.
[0020]
【Example】
Next, examples of the present invention will be described.
First, a medium having the following composition was prepared.
[0021]
Medium Peptone 5g
5g meat extract
NaCl 2.5g
500 ml of water (pH 7.0)
The medium having the above composition was placed in a 1000 ml container, Bacillus sp. CCC-a was inoculated therein, stirred well, allowed to stand, and cultured at 30 ° C. for 3 days.
[0022]
The obtained culture broth was centrifuged to separate the cells, washed thoroughly, and attached to a carrier to obtain a powder. The carrier used was diatomaceous earth baked and pulverized.
[0023]
This powdery Bacillus sp. CCC-a was inoculated into the object to be weighed, and the changes over time in COD, total nitrogen amount and total phosphorus amount were measured. The test was carried out according to JIS 0102-17 for COD, JIS 0102-45.2 for total nitrogen, and JIS 0102-46.2 for total phosphorus. As an object to be measured, a solution obtained by adding 0.2 g of starch per liter to 100 times of milk in seawater was used. The one as it was was used as a comparative example, and the one inoculated with Bacillus sp. CCC-a was used as an example. The test was carried out at 20 ° C. Moreover, in measuring each amount, the liquid to be measured was allowed to stand for 2 hours, and the supernatant was used as a test liquid.
[0024]
The test results are shown in Table 1. In the test, two samples were prepared for each, and the average was used as the measured value.
[0025]
[Table 1]
As can be seen from Table 1, it was found that Bacillus sp. CCC-a, which is a novel microorganism of the present invention, has a water purification function and an action of removing metals. Furthermore, the powder of Bacillus sp. CCC-a was sprinkled on a single rotten cocoon, and the odor was clearly reduced after 2 hours. In addition, Bacillus sp. CCC-a was sprinkled on fresh strawberries and stored at 25 ° C., and no odor occurred even after 48 hours. On the other hand, the strawberry stored at 25 ° C. without being sprinkled with Bacillus sp. CCC-a became quite odorous after 24 hours.
[0027]
Thus, Bacillus sp. CCC-a of the present invention also has a deodorizing action and a freshness preserving action.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12015797A JP3769098B2 (en) | 1997-04-23 | 1997-04-23 | New microorganisms belonging to the genus Bacillus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12015797A JP3769098B2 (en) | 1997-04-23 | 1997-04-23 | New microorganisms belonging to the genus Bacillus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH10295367A JPH10295367A (en) | 1998-11-10 |
| JP3769098B2 true JP3769098B2 (en) | 2006-04-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP12015797A Expired - Lifetime JP3769098B2 (en) | 1997-04-23 | 1997-04-23 | New microorganisms belonging to the genus Bacillus |
Country Status (1)
| Country | Link |
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| JP (1) | JP3769098B2 (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5072153B2 (en) * | 2001-09-07 | 2012-11-14 | 株式会社東京バイオックス | Antifouling agent for urinal drainage system and method for preventing urinal drainage system dirt using the same |
| KR101106030B1 (en) * | 2007-05-04 | 2012-01-17 | 주식회사 평강특장자동차 | Bacillus megaterium bc1-1 kccm 10856p reducing nasty odor and heavy metal and clarification methods for resource recovery from food waste or livestock waste water by using it |
| KR101106032B1 (en) * | 2007-05-04 | 2012-01-17 | 주식회사 평강특장자동차 | Bacillus cereus bc3 kccm 10859p reducing nasty odor and heavy metal and clarification methods for resource recovery from food waste or livestock waste water by using it |
| KR101106033B1 (en) * | 2007-05-04 | 2012-01-17 | 주식회사 평강특장자동차 | Bacillus clausii bc7 10863p reducing nasty odor and heavy metal and clarification methods for resource recovery from food waste or livestock waste water by using it |
| KR101106035B1 (en) * | 2007-05-04 | 2012-01-17 | 주식회사 평강특장자동차 | Bacillus firmus bc9 10865p reducing nasty odor and heavy metal and clarification methods for resource recovery from food waste or livestock waste water by using it |
| KR101106037B1 (en) * | 2007-05-04 | 2012-01-17 | 주식회사 평강특장자동차 | Bacillus licheniformis bc4 kccm 10860p reducing nasty odor and heavy metal and clarification methods for resource recovery from food waste or livestock waste water by using it |
| KR101106034B1 (en) * | 2007-05-04 | 2012-01-17 | 주식회사 평강특장자동차 | Bacillus sphaericus bc6 kccm 10862p reducing nasty odor and heavy metal and clarification methods for resource recovery from food waste or livestock waste water by using it |
| US10052399B2 (en) | 2014-08-25 | 2018-08-21 | Japan Environmental Science Company | Composition, support, wastewater treatment system, wastewater treating method, deodorization method, and batch wastewater treating method |
| CN113755405B (en) * | 2021-10-15 | 2023-06-20 | 南宁师范大学 | Bacillus bacterium F12 and its application |
-
1997
- 1997-04-23 JP JP12015797A patent/JP3769098B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPH10295367A (en) | 1998-11-10 |
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