JP4050560B2 - Vascular endothelial growth factor production promoter - Google Patents
Vascular endothelial growth factor production promoter Download PDFInfo
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- JP4050560B2 JP4050560B2 JP2002205071A JP2002205071A JP4050560B2 JP 4050560 B2 JP4050560 B2 JP 4050560B2 JP 2002205071 A JP2002205071 A JP 2002205071A JP 2002205071 A JP2002205071 A JP 2002205071A JP 4050560 B2 JP4050560 B2 JP 4050560B2
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- growth factor
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Description
【０００１】
【発明の属する技術分野】
本発明は、血管内皮細胞増殖因子の産生を促進する血管内皮細胞増殖因子産生促進剤に関し、さらに詳しくは、創傷治癒，肌色改善，育毛・養毛において、優れた効果を発揮するタコノキ属（Ｐａｎｄａｎｕｓ Ｌ． ｆ．）植物抽出物を有効成分とする血管内皮細胞増殖因子産生促進剤に関する。
【０００２】
【従来の技術】
近年の創傷治癒，肌色改善，育毛・養毛の研究分野では、血管新生を促進する血管内皮細胞増殖因子に注目が集まり、現在も様々な研究が進んでいる。炎症後の創傷部位では、毛細血管の新生により組織の修復が開始されることが知られており、血管内皮細胞増殖因子は、炎症或いは創傷治癒時の表皮において非常に高いレベルで発現し、血管のない表皮のために真皮での血管の増加と栄養供給を導いていることが報告されている。また、血管内皮細胞増殖因子は、皮膚の血管新生だけではなく、毛髪の成長にも大きく関与しており、毛包細胞において毛髪の成長期に発現し、毛髪の成長を促進していることが報告されている。
【０００３】
このことから、真皮での血管の増加と栄養供給に関与している血管内皮細胞増殖因子の産生を促進することは、創傷の回復・治癒に非常に有効であるばかりでなく、皮膚の新陳代謝の低下等によって生じるクスミや肌の透明感の低下といった肌色改善にも有効であり、また、毛髪・毛包の成長にも関与している血管内皮細胞増殖因子の産生を促進することは、毛髪の脱毛・薄毛といった症状の防止や改善にも有効であると考えられている。このため、様々な血管内皮細胞増殖因子産生促進剤がこれまでに開発されている。従来の血管内皮細胞増殖因子産生促進剤としては、大豆由来の調製物（特開平１１−２８６４３２），アミハナイグチ，シロヌメリイグチ，ハナイグチ，ウツロベニハナイグチ，アミタケ，キノボリイグチ，エゾウコギ，黄精，ゲンチアナ，センナ，トチュウ，ダイオウ，メリロート，ヨクイニン，クコの実，当帰，地黄，サンシシ，甘草，ニンジン，紅参，紫根，シンビジュームから選ばれる抽出物（特開２０００−２１２０５９）が開示されている。
【０００４】
しかし、これらの血管内皮細胞増殖因子産生促進剤は、副作用の点から配合が制限される場合があり、また有効量を配合すると着色や不快臭が発生するなどの問題が生じる場合もあった。
【０００５】
【発明が解決しようとする課題】
そこで、本発明においては、創傷治癒，肌色改善，育毛・養毛に優れた効果を発揮し、医薬品，医薬部外品，化粧料，食品などに応用可能な血管内皮細胞増殖因子産生促進剤を提供することを目的とした。
【０００６】
【課題を解決するための手段】
上記課題を解決するために、血管内皮細胞増殖因子の産生促進作用に基づき研究を重ねた結果、タコノキ属（Ｐａｎｄａｎｕｓ Ｌ． ｆ．）植物抽出物が優れた血管内皮細胞増殖因子産生促進作用を有することを見出し、さらに検討を加え、本発明を完成するに至った。すなわち、本発明は、タコノキ属（Ｐａｎｄａｎｕｓ Ｌ． ｆ．）植物抽出物を有効成分とする血管内皮細胞増殖因子産生促進剤に関するものである。
【０００７】
【発明の実施の形態】
本発明に用いられるタコノキ属（Ｐａｎｄａｎｕｓ Ｌ． ｆ．）植物は、タコノキ科の植物で、熱帯，亜熱帯地域に広く分布しており、日本では、南西諸島等でみられる。海岸近くに生育し、木質の茎から分岐しない放射状に出た気根を有し、扇形で長い葉をもつ。多くの種の葉が編物材料として利用されており、また果実や種子が食用にされる種もある。タコノキ属（Ｐａｎｄａｎｕｓ Ｌ． ｆ．）植物としては、アダン（Ｐａｎｄａｎｕｓ ｔｅｃｔｏｒｉｕｓ Ｓｏｌａｎｄ． ｅｘ Ｐａｒｋｉｎｓ．），タコノキ（Ｐａｎｄａｎｕｓ ｂｏｎｉｎｅｎｓｉｓ Ｗｅｂｅｒ），ビヨウタコノキ（Ｐａｎｄａｎｕｓ ｕｔｉｌｉｓ ＢＯＲＹ）等が日本では知られている。これら以外にも、アンダマンやニコマン島に分布するパンダヌス アンダマネンシウム（Ｐａｎｄａｎｕｓ ａｎｄａｍａｎｅｎｓｉｕｍ Ｋｕｒｚ），ニューギニアに分布するパンダヌス コノイデア（Ｐａｎｄａｎｕｓ ｃｏｎｏｉｄｅａ Ｌａｍｃｋ．），マダガスカルに分布するパンダヌス エデュリス（Ｐａｎｄａｎｕｓ ｅｄｕｌｉｓ Ｔｈｏｕａｒｓ），マレーシアに分布するパンダヌス オドルス（Ｐａｎｄａｎｕｓ ｏｄｏｒｕｓ Ｒｉｄｌ．）等が知られている。
【０００８】
これらタコノキ属（Ｐａｎｄａｎｕｓ Ｌ． ｆ．）植物の抽出物は、常法により得ることが出来る。抽出には、タコノキ属（Ｐａｎｄａｎｕｓ Ｌ． ｆ．）植物の幹，枝，果実，葉，花，種子，樹皮，樹液，根，芽等のいずれの部位を用いても構わないが、簡便に利用するには、葉，果実，種子，幼木の全草を用いるとよい。抽出の際は、生のまま用いてもよいが、抽出効率を考えると、細切，乾燥，粉砕等の処理を行った後に抽出を行うことが好ましい。抽出は、抽出溶媒に浸漬するか、超臨界流体や亜臨界流体を用いた抽出方法でも行うことができる。抽出効率を上げるため、撹拌や抽出溶媒中でホモジナイズしてもよい。抽出温度としては、５℃程度から抽出溶媒の沸点以下の温度とするのが適切である。抽出時間は抽出溶媒の種類や抽出温度によっても異なるが、１時間〜１４日間程度とするのが適切である。
【０００９】
抽出溶媒としては、水の他、メタノール，エタノール，プロパノール，イソプロパノール等の低級アルコール、１，３−ブチレングリコール，プロピレングリコール，ジプロピレングリコール，グリセリン等の多価アルコール、エチルエーテル，プロピルエーテル等のエーテル類、酢酸エチル，酢酸ブチル等のエステル類、アセトン，エチルメチルケトン等のケトン類等の溶媒を用いることができ、これらより１種又は２種以上を選択して用いる。また、生理食塩水，リン酸緩衝液，リン酸緩衝生理食塩水等を用いてもよい。さらに、水や二酸化炭素，エチレン，プロピレン，エタノール，メタノール，アンモニア等の１種又は２種以上の超臨界流体や亜臨界流体を用いてもよい。
【００１０】
タコノキ属（Ｐａｎｄａｎｕｓ Ｌ． ｆ．）植物の上記溶媒による抽出物は、そのままでも使用することができるが、濃縮，乾固した物を水や極性溶媒に再度溶解したり、或いはこれらの生理作用を損なわない範囲で脱色，脱臭，脱塩等の精製処理を行ったり、カラムクロマトグラフィー等による分画処理を行った後に用いてもよい。タコノキ属（Ｐａｎｄａｎｕｓ Ｌ． ｆ．）植物の前記抽出物やその処理物及び分画物は、各処理及び分画後に凍結乾燥し、用時に溶媒に溶解して用いることもできる。また、リポソーム等のベシクルやマイクロカプセル等に内包させて用いることもできる。
【００１１】
本発明における血管内皮細胞増殖因子産生促進剤は、タコノキ属（Ｐａｎｄａｎｕｓ Ｌ． ｆ．）植物抽出物を有効成分とする。本発明の血管内皮細胞増殖因子産生促進剤は、医薬品，医薬部外品，化粧料，食品などに配合して用いることができ、これらに用いることによっても創傷治癒，肌色改善，育毛・養毛といった効果が期待できる。
【００１２】
本発明に係る血管内皮細胞増殖因子産生促進剤は、経口剤，外用剤，注射剤，吸入剤，点鼻・点眼剤等の医薬品に配合することができ、これらの使用方法に応じて、錠剤，液剤，注射剤，軟膏，クリーム，ローション，エアゾール剤，座剤等の種々の剤型で提供することができる。なお、必要に応じて、賦形剤，基剤，乳化剤，安定剤，溶解助剤，矯味剤，保存剤，芳香剤，着色剤，コーティング剤等を適宜配合することができ、本発明の効果を損なわない範囲において、他の血管内皮細胞増殖因子産生促進剤との併用も可能である。
【００１３】
また、本発明に係る血管内皮細胞増殖因子産生促進剤は、皮膚外用剤，洗浄剤，メイクアップ化粧料，頭皮頭髪用化粧料等の医薬部外品や化粧料に配合することができ、これらの使用方法に応じて、ローション，乳液，ゲル，クリーム，軟膏剤，粉末，顆粒等の種々の剤型で提供することができる。なお、必要に応じて、医薬部外品，皮膚化粧料，頭皮頭髪用化粧料及び洗浄料に配合される、油性成分，保湿剤，粉体，色素，乳化剤，可溶化剤，洗浄剤，紫外線吸収剤，増粘剤，薬剤，香料，樹脂，防菌防黴剤，植物抽出物，アルコール類等を適宜配合することができ、本発明の効果を損なわない範囲において、他の血管内皮細胞増殖因子産生促進剤との併用も可能である。
【００１４】
さらに、本発明に係る血管内皮細胞増殖因子産生促進剤は、食品に配合することができ、これらの使用方法に応じて、ガムやキャンディーのような口腔用組成物、かまぼこ，ちくわ等の水産練り製品、ソーセージ，ハム等の畜産製品、洋菓子類、和菓子類、生麺，ゆで麺等の麺類、ソース，しょう油，たれなどの調味料、漬け物、総菜、清涼飲料水等一般的な飲食品の剤型とすることができる。その際、本発明の効果を損なわない範囲内で、食品に一般的に用いられる各種成分、例えば、砂糖，練乳，小麦粉，ショートニング，食塩，ブドウ糖，鶏卵，バター，マーガリン，水飴，カルシウム，鉄分，調味料，香辛料等を適宜配合することができ、本発明の効果を損なわない範囲において、他の血管内皮細胞増殖因子産生促進剤との併用も可能である。
【００１５】
【実施例】
さらに実施例により、本発明の特徴について詳細に説明する。まず、本発明のタコノキ属（Ｐａｎｄａｎｕｓ Ｌ． ｆ．）植物抽出物を含有する血管内皮細胞増殖因子産生促進剤の調製方法について示す。
【００１６】
［調製方法１］
タコノキ属（Ｐａｎｄａｎｕｓ Ｌ． ｆ．）植物の乾燥粉砕物１ｋｇに５０重量％エタノール水溶液を１０リットル加え、室温で７日間浸漬した。抽出液をろ過して回収し、溶媒を除去した後、タコノキ属（Ｐａｎｄａｎｕｓ Ｌ． ｆ．）植物抽出物を得た。
【００１７】
［調製方法２］
タコノキ属（Ｐａｎｄａｎｕｓ Ｌ． ｆ．）植物の乾燥粉砕物１ｋｇに水を９リットル加え、９０℃にて６時間還流して抽出した。抽出液をろ過して回収し、溶媒を除去した後、タコノキ属（Ｐａｎｄａｎｕｓ Ｌ． ｆ．）植物抽出物を得た。
【００１８】
［調製方法３］
タコノキ属（Ｐａｎｄａｎｕｓ Ｌ． ｆ．）植物の乾燥粉砕物１ｋｇにメタノールを９リットル加え、室温で７日間浸漬した。抽出液をろ過して回収し、溶媒を除去した後、タコノキ属（Ｐａｎｄａｎｕｓ Ｌ． ｆ．）植物抽出物を得た。
【００１９】
［調製方法４］
超臨界抽出装置にタコノキ属（Ｐａｎｄａｎｕｓ Ｌ． ｆ．）植物を投入し、４０℃において１５ＭＰａの大気圧下で二酸化炭素の超臨界流体を用いて抽出した。抽出物を回収し、タコノキ属（Ｐａｎｄａｎｕｓ Ｌ． ｆ．）植物抽出物を得た。
【００２０】
次に、タコノキ属（Ｐａｎｄａｎｕｓ Ｌ． ｆ．）の血管内皮細胞増殖因子の産生促進効果の評価について示す。評価には、タコノキ属（ＰａｎｄａｎｕｓＬ． ｆ．）植物のアダン（Ｐａｎｄａｎｕｓ ｔｅｃｔｏｒｉｕｓ Ｓｏｌａｎｄ． ｅｘ Ｐａｒｋｉｎｓ．）の葉から調製方法１によって得られたアダン抽出物を血管内皮細胞増殖因子産生促進剤の実施例１として用いた。
【００２１】
評価は、以下の手順で行った。正常ヒト表皮細胞を１ウェル当たり２．０×１０４個となるように９６穴マイクロプレートに播種した。播種培地には、市販のクラボウ社製Ｈｕｍｅｄｉａ−ＫＧ２を用いた。３７℃にて２４時間培養後、実施例１を任意の濃度で添加した試験培地に交換し、さらに２４時間培養し、培養上清中に分泌された血管内皮細胞増殖因子量をＥＬＩＳＡ法（Ｅｎｚｙｍｅ−ｌｉｎｋｅｄ ｉｍｍｕｎｏｓｏｒｂｅｎｔ ａｓｓａｙ）により定量した。また、陰性コントロールとして、血管内皮細胞増殖因子産生促進剤が無添加の培地を用い、陽性コントロールとして、血管内皮細胞増殖因子産生促進剤である塩化コバルトを０．２５ｍＭ含有する培地を用いて、それぞれのコントロールについての血管内皮細胞増殖因子量についてもＥＬＩＳＡ法により定量を行った。これらの評価結果を、陰性コントロールの血管内皮細胞増殖因子量を１００とした場合の相対値にて表１に示す。
【００２２】
【表１】
表１より、実施例１を０．０２５〜０．２０ｍｇ／ｍＬ添加した場合に、血管内皮細胞増殖因子産生促進剤が無添加の陰性コントロールと比較して、危険率１％未満で有意な血管内皮細胞増殖因子産生促進効果が認められた。このことから、実施例１は優れた血管内皮細胞増殖因子産生促進効果を有することが明らかとなった。
【００２４】
【発明の効果】
以上詳述したように、本発明により、優れた血管内皮細胞増殖因子産生促進効果を発揮する血管内皮細胞増殖因子産生促進剤を得ることができた。また、この血管内皮細胞増殖因子産生促進剤は、創傷治癒，肌色改善，育毛・養毛などに有用な医薬品，医薬部外品，化粧料，食品などに応用することができる。[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a vascular endothelial growth factor production promoter that promotes the production of vascular endothelial growth factor. More specifically, the present invention relates to a genus Taconoki ( Pandanus) that exhibits excellent effects in wound healing, skin color improvement, hair growth and hair restoration. L. f.) Relates to a vascular endothelial growth factor production promoter comprising a plant extract as an active ingredient.
[0002]
[Prior art]
In recent research fields of wound healing, skin color improvement, hair growth and hair restoration, attention has been focused on vascular endothelial growth factor that promotes angiogenesis, and various researches are ongoing. In wound sites after inflammation, it is known that tissue repair is initiated by the formation of capillaries. Vascular endothelial growth factor is expressed at very high levels in the epidermis during inflammation or wound healing. It has been reported that the absence of epidermis leads to increased blood vessels and nutrient supply in the dermis. In addition, vascular endothelial growth factor is greatly involved in hair growth as well as skin angiogenesis, and it is expressed in hair follicle cells during hair growth and promotes hair growth. It has been reported.
[0003]
Therefore, promoting the production of vascular endothelial growth factor, which is involved in the increase of blood vessels and nutrient supply in the dermis, is not only very effective in wound healing and healing, but also in the metabolism of the skin. It is effective in improving skin color such as dark spots and skin transparency caused by a decrease, and promoting the production of vascular endothelial growth factor, which is also involved in hair and hair follicle growth, It is thought to be effective in preventing and improving symptoms such as hair loss and thinning hair. For this reason, various vascular endothelial growth factor production promoters have been developed so far. Examples of conventional vascular endothelial growth factor production promoters include soybean-derived preparations (Japanese Patent Laid-Open No. 11-286432), Amihanaiguchi, Shironumeiguchi, Hanaiguchi, Utsurobenainaichi, Amanitake, Kinobori iuchi, Ezokogi, Yellow, Gentian, Senna, An extract selected from Eucommia, Daio, Merirot, Yokuinin, wolfberry, Toki, ground yellow, Sanshishi, licorice, carrot, red ginseng, purple root, and symbium is disclosed (Japanese Patent Laid-Open No. 2000-212059).
[0004]
However, these vascular endothelial growth factor production promoters are sometimes limited in terms of side effects, and there are cases where coloring and unpleasant odors occur when an effective amount is added.
[0005]
[Problems to be solved by the invention]
Therefore, in the present invention, a vascular endothelial growth factor production promoter that exhibits excellent effects on wound healing, skin color improvement, hair growth / hair growth, and can be applied to pharmaceuticals, quasi drugs, cosmetics, foods, etc. The purpose was to provide.
[0006]
[Means for Solving the Problems]
In order to solve the above-mentioned problems, as a result of repeated studies based on the production promoting action of vascular endothelial growth factor, the plant extract of the genus Panconus L. f. Has an excellent production promoting action on vascular endothelial growth factor. As a result, the present inventors have completed the present invention. That is, the present invention relates to a vascular endothelial growth factor production promoter comprising a Pandanus L. f. Plant extract as an active ingredient.
[0007]
DETAILED DESCRIPTION OF THE INVENTION
The genus Panconus L. f. Used in the present invention is a plant belonging to the family Taconaceae and is widely distributed in tropical and subtropical regions. In Japan, it is found in the Nansei Islands and the like. It grows near the coast, has aerial roots that radiate without branching from the woody stems, and has fan-shaped and long leaves. Many species of leaves are used as knitting materials, and there are also species where fruits and seeds are edible. The pandanus (Pandanus L. f.) Plant, Adan (Pandanus tectorius Soland. Ex Parkins. ), Pandanus (Pandanus boninensis Weber), etc. Biyoutakonoki (Pandanus utilis BORY) is in Japan are known. In addition to these, Pandanusu Anda imitate Nshi Umm distributed in the Andaman and Nikoman island (Pandanus andamanensium Kurz), distributed in New Guinea Pandanusu Konoidea (Pandanus conoidea Lamck.), Distributed in Madagascar Pandanusu Edeyurisu (Pandanus edulis Thouars), distribution in Malaysia Pandanus odorus Ridl. Is known.
[0008]
Extracts of these Panconus L. f. Plants can be obtained by conventional methods. For extraction, any part of the trunk, branch, fruit, leaf, flower, seed, bark, sap, root, bud, etc. of the plant of the genus Panconus L. f. To do this, use leaves, fruits, seeds, and whole plants of young trees. In the extraction, it may be used as it is, but considering the extraction efficiency, it is preferable to perform the extraction after performing processing such as shredding, drying, and pulverization. The extraction can be performed by immersing in an extraction solvent or by an extraction method using a supercritical fluid or a subcritical fluid. In order to increase the extraction efficiency, the mixture may be homogenized in stirring or an extraction solvent. The extraction temperature is suitably about 5 ° C. to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but it is appropriate to set it to about 1 hour to 14 days.
[0009]
Extraction solvents include water, lower alcohols such as methanol, ethanol, propanol, and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol, and glycerin, and ethers such as ethyl ether and propyl ether. , Solvents such as esters such as ethyl acetate and butyl acetate, and ketones such as acetone and ethyl methyl ketone can be used, and one or more of these can be selected and used. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used. Furthermore, you may use 1 type, or 2 or more types of supercritical fluids and subcritical fluids, such as water, a carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia.
[0010]
Extracts of the above-mentioned solvent of the genus Pandanus L. f. Can be used as they are, but the concentrated and dried solids can be dissolved again in water or a polar solvent, or their physiological action can be improved. It may be used after performing purification treatments such as decolorization, deodorization, desalting, etc. within a range that does not impair, or after performing fractionation treatment by column chromatography or the like. The extract of the genus Panconus L. f. And its treated product and fractions can be freeze-dried after each treatment and fractionation and dissolved in a solvent at the time of use. It can also be used by encapsulating in vesicles such as liposomes or microcapsules.
[0011]
The vascular endothelial growth factor production promoter in the present invention comprises a plant extract of Pandanus L. f. As an active ingredient. The vascular endothelial cell growth factor production promoter of the present invention can be used in combination with pharmaceuticals, quasi-drugs, cosmetics, foods, etc., and also by using them, wound healing, skin color improvement, hair growth / hair growth Such an effect can be expected.
[0012]
The vascular endothelial growth factor production promoter according to the present invention can be blended in pharmaceuticals such as oral preparations, external preparations, injections, inhalants, nasal drops and eye drops, and depending on the method of use, tablets , Liquids, injections, ointments, creams, lotions, aerosols, suppositories, and the like. If necessary, excipients, bases, emulsifiers, stabilizers, solubilizers, flavoring agents, preservatives, fragrances, colorants, coating agents and the like can be appropriately blended, and the effects of the present invention Can be used in combination with other vascular endothelial growth factor production promoter.
[0013]
Further, the vascular endothelial growth factor production promoter according to the present invention can be incorporated into quasi-drugs and cosmetics such as external preparations for skin, detergents, makeup cosmetics, cosmetics for scalp and hair, and the like. Can be provided in various dosage forms such as lotions, emulsions, gels, creams, ointments, powders, granules and the like. In addition, oily ingredients, moisturizers, powders, pigments, emulsifiers, solubilizers, detergents, ultraviolet rays, which are blended in quasi drugs, skin cosmetics, scalp and hair cosmetics and detergents as necessary. Absorbers, thickeners, drugs, fragrances, resins, antibacterial and antifungal agents, plant extracts, alcohols, and the like can be added as appropriate, and other vascular endothelial cell proliferations as long as the effects of the present invention are not impaired Combination with a factor production promoter is also possible.
[0014]
Furthermore, the vascular endothelial cell growth factor production promoter according to the present invention can be blended into foods, and depending on the method of use, oral compositions such as gums and candies, fish paste products such as kamaboko and chikuwa Livestock products such as sausages and ham, Western confectionery, Japanese confectionery, noodles such as raw noodles, boiled noodles, seasonings such as sauces, soy sauce, sauces, pickles, prepared dishes, soft drinks, etc. It can be. At that time, within the range not impairing the effect of the present invention, various components commonly used in foods, for example, sugar, condensed milk, flour, shortening, salt, glucose, chicken egg, butter, margarine, starch syrup, calcium, iron, A seasoning, a spice, etc. can be mix | blended suitably and can use together with another vascular endothelial growth factor production promoter in the range which does not impair the effect of this invention.
[0015]
【Example】
Further, the features of the present invention will be described in detail by way of examples. First, a method for preparing a vascular endothelial growth factor production promoter containing the plant extract of the genus Panconus L. f. Of the present invention will be described.
[0016]
[Preparation Method 1]
10 liters of a 50 wt% aqueous ethanol solution was added to 1 kg of a dried and pulverized plant of the genus Pandanus L. f. And immersed for 7 days at room temperature. The extract was collected by filtration, and after removing the solvent, a plant extract of Pandanus L. f. Was obtained.
[0017]
[Preparation Method 2]
Nine liters of water was added to 1 kg of a dry pulverized product of the genus Panconus L. f., And the mixture was extracted by refluxing at 90 ° C. for 6 hours. The extract was collected by filtration, and after removing the solvent, a plant extract of Pandanus L. f. Was obtained.
[0018]
[Preparation Method 3]
Nine liters of methanol was added to 1 kg of a dry pulverized product of the genus Panconus L. f., And immersed for 7 days at room temperature. The extract was collected by filtration, and after removing the solvent, a plant extract of Pandanus L. f. Was obtained.
[0019]
[Preparation Method 4]
A supercritical extractor was charged with Pandanus L. f. Plants and extracted using a supercritical fluid of carbon dioxide at 40 ° C. under an atmospheric pressure of 15 MPa. The extract was collected to obtain a plant extract of Pandanus L. f.
[0020]
Next, evaluation of the production promotion effect of vascular endothelial growth factor of the genus Taconoki ( Pandanus L. f.) Is shown. For evaluation, an example of an agent for promoting the production of vascular endothelial growth factor was obtained by using the Adan extract obtained by the preparation method 1 from the leaves of Pandanus L. f. Plant Adan ( Pandanus tectorius Soland. Ex Parkins.). Used as 1.
[0021]
The evaluation was performed according to the following procedure. Normal human epidermal cells were seeded in a 96-well microplate at 2.0 × 10 4 cells per well. As a seeding medium, commercially available Humdia-KG2 manufactured by Kurabo Industries Co., Ltd. was used. After culturing at 37 ° C. for 24 hours, the test medium was replaced with a test medium supplemented with an arbitrary concentration of Example 1, and further cultured for 24 hours. The amount of vascular endothelial cell growth factor secreted into the culture supernatant was determined by ELISA (Enzyme). -Linked immunosorbent assay). Further, as a negative control, a medium containing no vascular endothelial growth factor production promoter was used, and as a positive control, a medium containing 0.25 mM cobalt chloride, a vascular endothelial growth factor production promoter, The amount of vascular endothelial growth factor as a control was also determined by ELISA. These evaluation results are shown in Table 1 as relative values when the amount of vascular endothelial growth factor of the negative control is 100.
[0022]
[Table 1]
From Table 1, when Example 1 was added at 0.025 to 0.20 mg / mL, significant blood vessels were observed with a risk rate of less than 1%, compared with a negative control to which no vascular endothelial growth factor production promoter was added. An effect of promoting endothelial cell growth factor production was observed. From this, it became clear that Example 1 has an excellent vascular endothelial growth factor production promoting effect.
[0024]
【The invention's effect】
As described above in detail, according to the present invention, a vascular endothelial growth factor production promoter exhibiting an excellent vascular endothelial growth factor production promoting effect could be obtained. Moreover, this vascular endothelial cell growth factor production promoter can be applied to pharmaceuticals useful for wound healing, skin color improvement, hair growth and hair restoration, quasi drugs, cosmetics, foods, and the like.
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| EP2411032B1 (en) * | 2009-03-27 | 2014-07-09 | Evoria GmbH | Plant extracts for the treatment of skin diseases |
| DE102009022046A1 (en) * | 2009-05-16 | 2010-11-18 | Evoria Gmbh | Decoloured extracts of the plant Pandanus conoideus |
| JP6257876B2 (en) | 2010-07-02 | 2018-01-10 | 花王株式会社 | VEGF production promoter |
| JPWO2012096257A1 (en) * | 2011-01-12 | 2014-06-09 | 株式会社ライフアートビレッジ | Hair restorer |
| TWI706786B (en) * | 2018-12-26 | 2020-10-11 | 大江生醫股份有限公司 | Use of pandanus conoideus fruit extracts for suppressing gene expression in retina cells |
| JP6978015B1 (en) * | 2020-06-29 | 2021-12-08 | 徹 中西 | Composition for skin application to increase the expression of CCN1 and CCN2 |
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