JP4098110B2 - Cosmetics containing cellular active substances - Google Patents

Description

[0001]
TECHNICAL FIELD OF THE INVENTION
The present invention relates to a cosmetic and a food containing a cellular active substance contained in deep sea water. More specifically, the present invention relates to a skin cell active substance (including a fibroblast active substance such as a fibroblast energy metabolizing active substance and a fibroblast proliferating active substance), an immune cell active substance contained in deep ocean water. The present invention relates to cosmetics and foods containing cellular active substances such as (including eosinophil active substances). In the present invention, cosmetics include those used for the purpose of treating diseases by application or ingestion, and foods include those intended for treatment of diseases by ingestion. Shall be.
[0002]
TECHNICAL BACKGROUND OF THE INVENTION
In the human skin, aging symptoms such as wrinkles, blemishes and sagging appear due to internal factors such as aging or external factors such as ultraviolet rays. Such aging symptoms of the human skin are considered to be caused mainly by a decrease in the function of the cutaneous dermal fibroblasts, and the insufficient secretion of matrix fibers and collagen accompanying the decrease in the function of these cells. Therefore, it is considered that an effective means for preventing the aging of human skin or improving the function of aging skin is to activate skin dermal fibroblasts and further proliferate these cells.
[0003]
Extracts from seaweed and the like are said to be effective for such aging symptoms of human skin, that is, a decrease in the activity of skin dermal fibroblasts. For example, Patent Document 1 (Japanese Patent Application Laid-Open No. 10-330281) includes a group consisting of Yakuzusa, Hondara, Fukurimamiji, Fukuronori, Kombu, Kamensou, Kaikekenori, Japanese pterosa, Willow, Sozo, and Rattle. It is described that the extract activates the metabolism of dermal fibroblasts. Patent Document 2 (Japanese Patent Application Laid-Open No. 11-116431) discloses an invention for a topical skin preparation containing a culture supernatant of Keatoceros gracilis. As used herein, Chaetoceros gracilis is a kind of algae that lives alone in the waters near the sea. It is described that the culture supernatant of this ketoceros gracilis has an effect of improving the metabolic activity of dermal fibroblasts.
[0004]
In addition to these seaweed-derived components, for example, Patent Document 3 (Japanese Patent Application Laid-Open No. 2000-169320) includes any one of seaweed extract, chamomile extract, mugwort extract, Tamatsuki tsukudafuji extract, It is described that the combination with the yucca extract is effective as a cell activator.
However, such an algae or plant extract may cause fluctuations in the composition of the components extracted depending on the algae or plant habitat, and the extraction operation from such algae or plants is also complicated. There is.
[0005]
Patent Document 4 (Japanese Patent Application Laid-Open No. 2000-159654) discloses at least one selected from treatment methods such as ultrafiltration, reverse osmosis membrane filtration, electrodialysis, ion exchange membrane, and distillation of deep sea water. An invention of cosmetics characterized in that it contains concentrated water obtained by treatment by various treatment methods is disclosed. This publication describes that the conductivity of this concentrated water is 5 mS / cm to 1 S / cm, preferably 10 mS / cm to 500 mS / cm, and seawater has the above conductivity. Is considered to contain a considerable amount of ionic substances. In this publication, the total abundance ratio of chlorine ions, sodium ions and potassium ions per water-evaporated solid in the concentrated water is 35% or less, and the concentrated water actually used in Example 2 and Comparative Example 2 is used. The total abundance of chlorine ions, sodium ions and potassium ions in B1 is 31.5%, and the total abundance of chlorine ions, sodium ions and potassium ions in concentrated water B2 used in Example 2 and Comparative Example 2 is also 32. It shows a very high value of 0.5%. And in this gazette, it is the concentrated water which has these values and is obtained by the method described in this gazette, Comprising: It is irritating with respect to skin among the components contained in deep sea water. It is understood that the invention of cosmetics using deep ocean water containing a high concentration of minerals excluding chlorine ions, sodium ions and potassium ions is disclosed. When chlorine ions, sodium ions, and potassium ions that are irritating to the skin are removed from inorganic substances contained in deep sea water, phosphate ions, silicate ions, nitrate ions, etc., which are generally called nutrient salts The content ratio is increased, and it is considered that these natural minerals have an effect of improving skin dullness, wrinkles and the like and a slimming effect.
[0006]
As described above, Patent Document 5 (Japanese Patent Application Laid-Open No. 2000-159654) discloses makeup using concentrated water of deep sea water mainly containing natural minerals contained in deep sea water by concentrating deep sea water. It is understood that the effect exhibited by the cosmetic disclosed in this publication is a natural mineral component contained in deep sea water, that is, an inorganic salt.
[0007]
Apart from skin aging due to internal factors such as aging, patients who have various allergic symptoms such as atopic dermatitis due to changes in the living environment and eating habits, stress, etc. in recent years as skin diseases Is increasing and is a problem.
Allergic symptoms such as atopic dermatitis are a type of hypersensitivity in which the inherited constitution plays a major role. Antigens that cause allergic symptoms are thought to be in the blood, and the complex of this antigen and antibody releases histamine or histamine-like substances from tissues, resulting in specific symptoms It is believed that. For example, in atopic dermatitis, peripheral blood eosinophils increase, and infiltration of eosinophils is often seen in histological findings. This also suggests that eosinophils are one of the cells that play a central role in atopic dermatitis.
[0008]
Against this background, various studies have been conducted on eosinophil active substances, and it is already known that the activity of eosinophils is enhanced by deep ocean water. The mechanism for eosinophil activity has not been elucidated yet. At present, the idea that eosinophil activity is derived from minerals contained in the deep ocean water is the majority, and the mineral content obtained by concentrating, desalting and separating deep ocean water is large. The isolates contained were used for allergic drugs and the like.
[0009]
Most of the substances obtained by concentrating, desalting and separating the deep ocean water as described above are occupied by inorganic salts such as calcium sulfate. However, it is known that calcium sulfate has substantially no medicinal effects related to allergy.
[0010]
[Patent Document 1]
Japanese Patent Laid-Open No. 10-330281
[Patent Document 2]
JP 11-116431 A
[Patent Document 3]
JP 2000-169320 A
[Patent Document 4]
Japanese Unexamined Patent Publication No. 2000-159654
[Patent Document 5]
Japanese Unexamined Patent Publication No. 2000-159654
[0011]
OBJECT OF THE INVENTION
The present invention removes inorganic salts from deep ocean water as much as possible, while skin cell active substances (fibroblasts), which are cellular active substances composed of highly concentrated desalted ocean deep water in which organic substances contained in the ocean deep water are concentrated. Cosmetics using fibroblast active substances such as cell energy metabolism active substances and fibroblast proliferation active substances) and immune cell active substances (including eosinophil active substances) Charge It is intended to provide.
[0012]
SUMMARY OF THE INVENTION
Makeup of the present invention Charge , Makeup containing active ingredients and water At a charge The water undergoes an operation of separating at least a part of the inorganic salt dissolved in the deep ocean water. Concentrated concentration It is characterized by the fact that it is water containing cellular active substances consisting of salt ocean deep water.
[0013]
The makeup of the present invention Charge A cosmetic or pharmaceutical comprising an active ingredient and water, wherein the water undergoes an operation of separating at least a part of an inorganic salt dissolved in deep sea water. Concentrated concentration It is characterized by being cell-active substance-containing water that can be obtained by performing the operation of further concentrating salt ocean deep water at least once.
[0014]
Cosmetic products according to the present invention are for lotions, emulsions, creams, gels, whitening essences, UV protection essences, cosmetic oils, packs, shampoos, conditioning shampoos, rinses, hair restorers, hair liquids, liquid toothpastes, mouthwashes, wet naps. Any cosmetic selected from the group consisting of a medicinal solution, a pregnancy-prevention cream, a body tightening gel, and a bust massage gel.
[0015]
The deep ocean water contains inorganic salts and organic substances, and the concentration of the inorganic salt dissolved in the deep ocean water is usually about 3.4% by weight. Of these inorganic salts, about 2.2% by weight or more is sodium chloride, magnesium chloride or calcium sulfate, and these inorganic salts have little action as a fibroblast active substance, and particularly in inorganic salts. It is known that calcium sulfate contained in a large amount has substantially no medicinal effects related to allergy.
[0016]
On the other hand, when a concentrated solution obtained by concentration while removing inorganic salts dissolved in deep sea water was analyzed, it was found that the concentrated solution contained an organic substance having a certain molecular weight or more. The details of the origin and structure of such organic substances are not clear, and it is estimated that they contain a very wide variety of components. However, when such an organic substance is analyzed by selecting a developing solvent and using gel filtration chromatography, in the ultraviolet detection analysis at a wavelength of 280 nm, the first fraction having a molecular weight of 1 million or more and the second fraction having a molecular weight of 30,000 to 1,000,000 are used. It was found that an organic substance having a peak was contained in at least one fraction of the fraction, the third fraction having a molecular weight of 5000 to 30,000, and the fourth fraction having a molecular weight of 5000 or less.
[0017]
Then, when organic substances contained in such deep ocean waters were selectively collected and investigated for cellular activity such as behavior and eosinophil activity for fibroblasts, fibroblasts were added to these organic substances. There are cell activities such as energy metabolism / proliferation activity and eosinophil activity.
Therefore, cell energy metabolism is absorbed by using cellular active substance-containing water containing a high amount of organic substances contained in trace amounts in deep sea water as water to be blended in cosmetics. -Cell activity such as proliferation activity can be improved.
[0018]
DETAILED DESCRIPTION OF THE INVENTION
Next, the cosmetics and food of the present invention will be specifically described.
In the present invention, cell active substances include skin cell active substances and immune cell active substances, and skin cell active substances refer to substances that can activate fibroblasts and the like. In addition, immune cell active substances include leukocytes, ie, monocytes such as macrophages, lymphocytes such as NK cells, T cells, K cells, and B cells, granulocytes such as eosinophils, neutrophils, and basophils. A substance that can activate the like.
[0019]
In FIG. 1, the example of typical operation for obtaining the cell active substance containing water used by this invention is shown. In addition, although the cellular active substance containing water of this invention can be obtained by the said operation, respectively, it should not be limitedly interpreted to the substance obtained only by this operation.
First, the cell active substance-containing substance used in the present invention will be specifically described along the manufacturing flow illustrated in FIG.
[0020]
As shown in FIG. 1, in the present invention, cell-active substance-containing water is produced using deep ocean water as a raw material.
In the present invention, the cell-active substance-containing water contains skin cell active substances and / or immune cell active substances, particularly preferably fibroblast energy metabolism / proliferation active substances and / or eosinophil active substances. . That is, the cellular active substance-containing water used in the present invention may have skin cell activity and at the same time have immune cell activity, or only have skin cell activity or immune cell activity. Also good.
[0021]
In the present invention, deep ocean water having a depth of 200 m or more, preferably 300 m or more, at least where sunlight does not reach is used. Although the water depth is less than 200m, deep sea water that rises near the sea surface due to the rise of seawater can also be used.
Generally, the depth at which sunlight can reach in ocean water is about 200 m. Therefore, since sunlight does not naturally reach deep sea water that exists in the deep sea of about 200m or less, photosynthesis by algae or aquatic plants is not performed in this deep sea water, and plankton, etc. The general view is that there is almost no breeding. In this way, it is thought that organic substances are hardly contained in the ocean currents in the deep ocean, but on the other hand, the mineral content is higher than normal ocean water (meaning ocean water other than ocean deep water). These minerals also contain rare minerals that tend to be deficient in the current normal diet. It was also found that a lot of organic substances were dissolved in this deep ocean water. Therefore, it is highly possible that such dissolved organic substances have cell activity, and if such components can be obtained, high cell activity, specifically, fibroblast energy metabolism, Skin cell active substances such as proliferative active substances (fibroblast active substances) or immune cell active substances such as eosinophil active substances can be obtained. These fibroblast energy metabolism / proliferation active substances are effective in preventing skin aging or improving the function of aging skin, and these eosinophil active substances are very useful as therapeutic agents for allergic diseases such as atopy. High nature.
[0022]
The deep ocean water collected in this way is filtered using a filter having a relatively small opening, such as a membrane filter, in order to remove suspended matter that may be mixed during the collection. In the present invention, it is usually filtered using a filter having an opening of 0.01 to 2 μm, preferably 0.1 to 1 μm, particularly a membrane filter. By using such a filter, fine components that cannot be confirmed with the naked eye can be filtered out. If there are precipitates or inorganic salts that precipitate at this stage, such precipitates may be separated at this stage.
[0023]
In the present invention, since the filtrate filtered as described above is used, the substance (residue) that could not pass through the filter is discarded.
In the present invention, the filtrate obtained as described above is distilled under reduced pressure, and at least a part of the water contained in the filtrate is removed by this reduced-pressure distillation operation. When the first deep sea water sampled is 100 parts by volume, the distillation operation under reduced pressure usually results in 1 to 50 parts by volume, preferably 1 to 30 parts by volume with respect to the first deep sea water sample. Further, the distillation under reduced pressure is carried out so that the amount is more preferably 2 to 25 parts by volume, particularly preferably 5 to 20 parts by volume. Specifically, under a reduced pressure of 1 to 40 torr, preferably 5 to 30 Torr, at a temperature of 50 ° C. or lower, preferably 40 ° C. or lower, 10 minutes to 3.0 hours, preferably 30 minutes to 1.0. Distill under reduced pressure over time. Note that the distillation temperature during this vacuum distillation is preferably as low as the above range in order to prevent decomposition of dissolved substances dissolved in deep sea water. Therefore, during the vacuum distillation used here, The vacuum distillation operation can be carried out at a lower temperature by lowering the pressure of. A part of the water in the deep sea water is separated by this vacuum distillation operation, and the separated water (fresh water) is discarded. Moreover, if the processing capacity of the facility for separating the inorganic salt is sufficient, the above-described concentration operation can be omitted.
[0024]
Moreover, instead of concentrating deep sea water by vacuum distillation and separating inorganic salts as described above, an electrolyte (inorganic salt) in deep sea water can be separated using a charged mosaic membrane. By using a charged mosaic membrane, it is possible to selectively remove inorganic salts in deep ocean water, and it is not necessary to heat the deep ocean water for selective removal of these inorganic salts. Therefore, it is possible to reduce the heat effect on the dissolved organic matter.
[0025]
On the other hand, inorganic salts and the like are deposited in the deep ocean water concentrated by separating water by vacuum distillation as described above (hereinafter referred to as concentrated ocean deep water). This precipitate is mainly composed of an inorganic salt such as sodium chloride, calcium sulfate or magnesium chloride, and preferably 2 to 10 after the distillation residue is sufficiently cooled (usually at room temperature (25 ° C.) or lower). The deposited precipitate is separated by cooling to ° C. A general separation method such as filtration, centrifugation, or decantation can be used to separate the precipitate. Moreover, these separation operations can be combined. The amount of precipitates (mainly inorganic chlorides) deposited by concentration under reduced pressure as described above is relatively large, and most of these precipitates are crystallized inorganic salts. It is efficient.
[0026]
Although there is no limitation in particular in the filtration apparatus used for isolation | separation of deposits, such as inorganic salt here, a filter with an opening of 0.01-2 micrometers normally, Preferably it is 0.1-1.0 micrometer is used. By filtering under reduced pressure using the above filter, the precipitate and the filtrate can be separated efficiently. As described above, the precipitates separated here are mainly inorganic chlorides, and inorganic salts such as inorganic chlorides (mainly sodium chloride and magnesium chloride) dissolved in the deep sea water used first by this separation operation. In addition, 50% by weight or more, preferably 70% by weight or more, particularly preferably 90% by weight or more of the calcium sulfate is removed. The precipitate (mainly inorganic chloride) separated as described above is discarded without being used in the present invention.
[0027]
In the present invention, when the precipitate is separated as described above, that is, when separated by filtration as shown in the above example, the above desalted filtrate is used in the next step (hereinafter referred to as (concentration) desalting. This is called salt ocean deep water).
That is, the cell-active substance-containing water used in the present invention may be the desalted filtrate ((concentrated) desalted deep sea water) obtained in the above-described step, and further this desalted filtrate ((concentrated). It may be water containing a high concentration of a cellular active substance that can be obtained by further concentrating (desalted deep ocean water) and separating the precipitate deposited by this concentration at least once.
[0028]
Here, the filtrate is usually concentrated by distillation under reduced pressure.
In the present invention, the concentration operation (process) refers to an operation for removing dissolved salts dissolved in ocean water. In this step, ocean water may be concentrated and fresh water may be removed. The pressure at this time can be normally set within the range of 1 to 40 Torr, preferably 5 to 30 Torr, and the distillation temperature is usually 5 to 50 ° C., preferably 10 to 40 ° C. In one operation under the above conditions, distillation is performed so that the amount of the original filtrate is preferably within the range of 5 to 30 parts by volume, more preferably 5 to 20 parts by volume with respect to 100 parts by volume. By distillation in this way, components (mainly inorganic salts) dissolved in the filtrate are deposited. Next, in the present invention, the precipitate (mainly inorganic salt) thus precipitated is separated.
[0029]
Separation of this precipitate or inorganic salt, that is, removal of dissolved salt, is performed by separation operation using a semi-permeable membrane, operation for electrically separating residual ionic substances, centrifuge operation, gel filtration operation, charged mosaic membrane. It is preferable to employ any one of the separation operation used and the ultrafiltration operation. That is, the precipitate in this process tends to have a smaller particle size than the precipitate in the previous process, and some of the precipitate may be colloidal or even gelled. Difficult to separate.
[0030]
Therefore, the separation of precipitates or inorganic salts and liquid in this step is a method of separating from the dispersion medium using the difference in osmotic pressure of the semi-permeable membrane, and the electrical properties of the remaining ionic substances by applying voltage. A method of collecting and separating from a dispersion medium using characteristics, a method of collecting a precipitate using an external stress such as centrifugal force and separating it from the dispersion medium, and a filtration membrane having a desired permeability such as an ultrafiltration membrane. The precipitate and the dispersion medium can be separated by using a method of filtering under pressure using a method, a method of separating low molecular weight particles from a dispersion medium using gel filtration, or the like.
[0031]
The cellular active substance-containing water used in the present invention is a dispersion medium (highly concentrated desalted deep ocean water) obtained by performing the above-described concentration operation and separation operation at least once, preferably at least twice. It is preferable. When the above steps are repeated, the concentration operation and separation operation performed in the previous operation and the subsequent concentration operation and separation operation do not need to be the same operation, and the state of the precipitates, etc. Corresponding operations can be combined as appropriate. The precipitate thus separated is considered to be mainly the remaining inorganic salt, dissolved metal compound, etc., and in the present invention, this precipitate is discarded.
[0032]
Cell active substance-containing water (highly concentrated desalted deep sea water) obtained by repeating concentration and separation as described above is a liquid containing a high concentration of cell active substances, and this cell active substance (ie, fiber) The amount of the liquid containing a high concentration of blast metabolite and eosinophil active substance is usually 0.00001 to 50.0 parts by volume, preferably 100 parts by volume of the deep sea water used first, preferably The amount is 0.0001 to 30.0 parts by volume, more preferably 0.001 to 5.0 parts by volume, and particularly preferably 0.01 to 0.5 parts by volume.
[0033]
The cell-active substance-containing water (highly concentrated desalted deep ocean water) obtained as described above contains various organic substances. Conversely, the content of inorganic substances is very low. Is 2.0% by weight or less, preferably 0.9% by weight or less, more preferably 0.1% by weight or less, and particularly preferably 0.01% by weight or less. Contains The ignition residue of water is usually 1.0% by weight or less, preferably 0.1% by weight or less, particularly preferably 0.01% by weight or less. In particular, the content of inorganic chloride in the cell-active substance-containing water (highly concentrated desalted deep ocean water) is extremely low and is usually 1000 ppm or less, preferably 100 ppm or less. Further, the total amount of organic substances contained in this cell-active substance-containing water (highly concentrated desalted deep ocean water) is usually 0.001 to 50% by weight, preferably 0.01 to 50% by weight, particularly Preferably it exists in the range of 0.1 to 10 weight%.
[0034]
In the cell-active substance-containing water (highly concentrated desalted deep ocean water) obtained as described above, for example, a skin cell active substance such as a substance that activates human skin fibroblasts, and / or For example, cell active substances such as immune cell active substances such as substances that enhance the activity of eosinophils are contained. Details of the skin cell active substance and immune cell active substance contained in this cell active substance-containing water are unknown, but at least the cell active substance-containing water obtained by treating deep sea water as described above is It contains a component that imparts energy metabolism / proliferation activity to fibroblasts and / or a component that has eosinophil activity. According to the study by the present inventor, it is estimated that this substance is any organic substance contained in the cell-active substance-containing water.
[0035]
Therefore, phosphate buffered saline (PBS-) was used as the developing solvent for this cell-active substance-containing water, and the product name Sephacryl S-300 (manufactured by Pharmacia) was used as the column, and gel filtration chromatography at a wavelength of 280 nm Analyzed by ultraviolet detection, the analysis results are divided into a first fraction having a molecular weight of 1 million or more, a second fraction having a molecular weight of 30,000 to 1 million, a third fraction having a molecular weight of 30,000 to 5000, and a molecular weight of 5000 or less. When divided into fourth fractions and examined for the presence or absence of peaks in each fraction, the highly concentrated desalted deep ocean water obtained as described above is at least one of the first to fourth fractions. At least one peak is observed in this fraction. A peak appearing in the fraction partitioned by molecular weight as described above means that the cell-active substance-containing water contains an organic compound having a molecular weight corresponding to the fraction. Incidentally, when Ribonuclease A having a number average molecular weight of 13700 is added as a labeling substance, the portion corresponding to Ribonuclease A appears in the third fraction, and when adding Ferritin having a number average molecular weight of 440000 as the labeling substance, The part corresponding to Ferritin appears in the second fraction.
[0036]
FIG. 2 shows an example of a gel filtration chromatography chart measured for the cellular active substance-containing water obtained as described above. In FIG. 2, the dashed lines indicate Ribonuclease A having a number average molecular weight of 13700 and Ferritin peaks having a number average molecular weight of 440000 used as the labeling substances.
In the present invention, the cell-active substance-containing water obtained as described above is used as water to be blended in cosmetics.
[0037]
That is, the cosmetics and foods of the present invention contain water, and conventionally, purified water is used as the water, but in the present invention, cell active substance-containing water is used instead of the purified water. use.
Specific examples of the cosmetics of the present invention include lotion, emulsion, cream, gel, whitening essence, UV protection essence, cosmetic oil, pack, shampoo, conditioning shampoo, rinse, hair restorer, hair liquid, liquid toothpaste, Examples thereof include mouthwashes, wet-up liquids, gestation line prevention creams, body tightening gels, and bust massage gels. As water contained therein, cell active substance-containing water is used.
[0038]
In addition to the above cosmetics, facial soaps such as solid soap, cleansing foam, cleansing gel, cleansing cream, cleansing milk, cleansing mask, cleansing oil, prior application powder and shaving foam; flexible lotion, astringent Lotions such as crystalline crystal water, washing lotion and multi-layer lotion; moisturizing, softening, blood circulation promotion, UV protection, makeup base, makeup base, keratin softening, hair protection, hair removal, hairdressing, deodorization, shaving, Oils, gels, emulsions and creams used for body, hand or massage; hack masks such as peel-off type, wipe-off type, wash-off type, peel-off type after solidification, pasting type and impregnation type Powder, white powder, dusting powder, foundation, lipstick, cheek , Eyeliner, mascara, eyebrow, nail enamel, eye shadow, enamel remover and nail treatment and other cap-cap cosmetics; hair foam, hair mousse, hair spray, hair gel, water grease, set lotion, pomade, tic, hair cream, treatment Cosmetics for hair such as lotion, split coat, hair oil, permanent wave agent, hair dye and hair bleach; whole body cleansing cosmetics such as soap, medicinal soap, liquid body shampoo, hand soap and foot soap; sunscreen Deodorant lotions, deodorant sprays, deodorant sticks, eau de cologne and perfumes Hygiene cosmetics such as depilation, depilation, bathing and insectopelper; Oral cosmetics such as toothpaste, mouthwash, deodorant, refreshment; wipe and application of wet nap, wet tissue, buttocks and cotton swabs Cotton impregnating chemicals; aerosol chemicals and the like.
[0039]
Cosmetics such as those mentioned above are effective as moisturizers, oils, surfactants, thickeners, solubilizers, colorants, fragrances, powder pairs, pH adjusters, antiseptic / antifungal agents, and other ingredients. It is formed from ingredients and water.
Examples of the food of the present invention include various beverages and retort foods.
[0040]
In the cosmetic and food of the present invention, water containing cellular active substances produced from deep sea water as described above is used in place of conventionally used water (generally purified water). This cell active substance-containing water contains skin cell active substances and immune cell active substances at high concentrations as described above, and cell active substance-containing water containing such components at high concentrations is used instead of water. By repeatedly using the used cosmetics, it is possible to improve skin metabolism, for example, by improving the energy metabolism of fibroblasts and promoting proliferation.
[0041]
The cosmetics and food of the present invention can be produced in the same manner as conventional cosmetics except that cell-active substance-containing water produced from deep ocean water is used instead of purified water. In the present invention, the amount of the cell-active substance-containing water can be appropriately set depending on the type of food or cosmetic, but the content of the cell-active substance-containing water in 100 parts by weight of the food or cosmetic of the present invention is usually 0. 0.1-99.9 parts by weight, preferably 0.1-99 parts by weight.
[0042]
For example, the lotion of the present invention is obtained by dissolving a moisturizer such as 1,3-ethylene glycol and glycerin, an emollient such as oleyl alcohol, a surfactant, and alcohol in water containing a cell active substance. .
Further, for example, the emulsion is obtained by emulsifying a moisturizing agent such as 1,3-butylene glycol, glycerin and PEG 4000 and an emollient such as olive oil in water containing a cell active substance using a surfactant and alcohol. .
[0043]
For example, O / W type cream is a cell-active substance using a surfactant together with an oil such as stearyl alcohol, stearic acid, hydrogenated lanolin and octyldecanol, and a moisturizer such as 1,3-butylene glycol and PEG500. It can be produced by dispersing it in a cream form in the contained water.
Further, for example, in the case of a moisturizing gel, a moisturizing agent such as dipropylene glycol or PEG 1500 is dispersed in a gel containing water containing a water-soluble polymer compound such as carboxyvinyl polymer or methyl cellulose and a surfactant. Is obtained.
[0044]
For example, whitening essence uses a surfactant with a moisturizer such as propylene glycol and PEG400, an alcohol such as ethanol, a water-soluble polymer, an emollient, and a drug such as placenta and vitamin E acetate. It is obtained by dispersing in active substance-containing water.
In addition, for example, UV-proof essence is an oil agent such as stearic acid, cetanol, lanolin derivatives, liquid paraffin, 2-ethylhexyl acrylate, dispersed in cell-containing substance-containing water using a surfactant together with a humectant and UV absorber. Is obtained.
[0045]
Further, for example, cosmetic oils can be obtained by emulsifying oils such as olive oil, liquid paraffin, squalane and drugs such as vitamin E acetate in water containing a cell active substance using a surfactant.
The pack can be obtained by dissolving a coating agent such as polyvinyl alcohol, a thickener such as carboxymethyl cellulose, and a moisturizing agent such as 1,3-butylene glycol in a cell-active substance-containing water together with a surfactant.
[0046]
For example, a shampoo is a mixture of a surfactant, a humectant and water containing a cellular active substance.
In addition, the hair restorer is, for example, ethyl alcohol and cell-active substance-containing water, assembly extract, vitamin B ₆ It can be obtained by dissolving hair growth such as vitamin E derivatives.
[0047]
The hair liquid is obtained by dissolving a hair styling agent such as polyoxypropylene (40) butyl ether or polyoxyethylene hydrogenated castor oil in water containing ethyl alcohol and a cellular active substance.
Liquid toothpaste can be obtained, for example, by dispersing silicic anhydride, propylene glycol, glycerin, sorbite solution, sodium polyacrylate, sodium lauryl sulfate, or saccharin sodium in water containing a cell active substance.
[0048]
Further, the mouthwash can be obtained, for example, by dissolving ethanol, glycerin, polyoxyethylene hydrogenated castor oil, saccharin name thulium, sodium benzoate and the like in water containing a cell active substance.
A wet tissue cosmetic liquid (impregnation liquid) can be obtained by dissolving a moisturizing agent such as propylene glycol or 1,3-butylene glycol in water containing a cell active substance.
[0049]
As described above, the cosmetic of the present invention uses cell-active substance-containing water obtained from deep ocean water instead of the conventionally used water, and this cell-active substance-containing water is obtained from deep ocean water. It is desalted and concentrated, and contains a high concentration of skin cell active substances and / or immune cell active substances. Therefore, by repeatedly using the cosmetic of the present invention produced using such cell-active substance-containing water, it is possible to improve skin metabolism and maintain the skin always in a healthy state.
[0050]
By using the external preparation for skin of the present invention containing the cell energy metabolism / proliferation active substance (fibroblast active substance) in the amount as described above, this cell energy metabolism / proliferation active substance (fibroblast active substance) Activates human skin cells, especially fibroblasts, and promotes secretion of anti-aging components such as collagen, for example, and can effectively prevent pigmentation and decrease in hyaluronic acid production. .
[0051]
Together, these can prevent aging symptoms such as wrinkles, blemishes and sagging in human skin, or treat and improve the skin that has fallen into aging symptoms.
Further, since the cellular active substance used in the present invention contains an eosinophil active substance, it is difficult to cause dermatitis, and it is also possible to cure the skin when there is inflammation.
[0052]
【The invention's effect】
The cell-active substance-containing water blended in the cosmetic or food of the present invention is water containing a substance contained in a solution obtained by subjecting deep sea water to a specific treatment at a high concentration.
Conventionally, it is known to use a separated substance from deep ocean water for skin treatment or allergy treatment, but these use inorganic components contained in deep ocean water. That is, deep sea water contains various minerals in addition to chlorides such as sodium chloride, calcium chloride and magnesium chloride, and these minerals are known to be used for skin treatment or allergy treatment. It was. Therefore, when using deep sea water for skin treatment and allergy treatment, the precipitate (mineral content) deposited by concentrating deep sea water is separated from seawater and used. Furthermore, since it was not thought that organic components effective for skin treatment or allergy treatment were contained in deep sea water, it was attempted to separate and use organic substances contained in deep sea water. It was not done.
[0053]
In contrast, the inventor of the present invention contains an organic substance in addition to the inorganic salt as described above in the deep sea water, and this organic substance has a function completely different from the mineral contained in the deep sea water. It has been found that by utilizing this function, skin cell activity such as energy metabolism and proliferation activity of fibroblasts forming the skin and immune cell activity such as eosinophil activity can be improved. is there.
[0054]
The cosmetic composition of the present invention uses cell active substance-containing water containing such a component having cellular activity at a high concentration in place of the water used in conventional cosmetics. The material can improve the energy metabolism / proliferation activity and eosinophil activity of fibroblasts by the action of organic substances contained in deep ocean water.
[0055]
Moreover, water containing cellular active substances used in the present invention (including skin cell active substances such as fibroblast energy metabolism / proliferation active substances and immune cell active substances such as eosinophil active substances) is usually used. Compared with other cell-active substances, it has extremely few side effects and is extremely safe. Specifically, fibroblasts exhibit excellent cell energy metabolism / proliferation activity (cell activity) without side effects. Therefore, it is suitable for blending into cosmetics used directly on the skin.
[0056]
More specifically, by incorporating the cell activity-containing water used in the present invention, the fibroblasts can be remarkably activated by the fibroblast active substance contained therein, so that the cells of human skin It is possible to improve the energy metabolism activity and proliferation activity of humans, and to prevent or treat aging phenomena such as wrinkles, blemishes and sagging in human skin.
[0057]
【Example】
Next, although an Example is shown and this invention is demonstrated further in detail, this invention is not limited by these.
[0058]
[Example 1]
[Preparation of water containing cellular active substances]
20 liters of deep ocean water was collected from a depth of 687m off Suruga Bay.
20 liters of this deep sea water was filtered using a membrane filter having an opening of 0.45 μm to remove suspended matters in this deep sea water.
[0059]
The filtered deep ocean water is put into a vacuum distillation apparatus, the distillation temperature is set to 40 ° C. or less under the pressure condition of 20 Torr, and the amount of deep ocean water is about 3.3 liters (the original ocean water) Distilled under reduced pressure until 1/6 of the deep water.
By distillation under reduced pressure in this way, inorganic salts such as sodium chloride, potassium chloride and magnesium chloride which had been dissolved in the deep ocean water were precipitated. The deposited inorganic salt was filtered through a membrane filter having an opening of 0.5 μm. The filtered inorganic salt was discarded because it is not used in the present invention.
[0060]
Next, the filtrate obtained in the above step is subjected to a desalting operation at a low temperature of 8 ° C. using a mosaic charged membrane (trade name: MC-55B mosaic charged membrane, manufactured by Tosoh Corporation). The resulting liquid is put into a vacuum distillation apparatus, and the temperature is controlled so as not to exceed the temperature of 40 ° C. under a reduced pressure of 20 Torr, and the volume of the liquid is 200 ml (approximately 1/100 of the original deep sea water). Water was distilled off under reduced pressure until.
[0061]
The desalting and vacuum distillation using the above mosaic charged membrane was repeated to obtain 5 ml of concentrate.
The concentrate contained 0.2% by weight of an organic substance, and the content of inorganic salt in the concentrate was 100 ppm or less, and the content of inorganic chloride was 70 ppm or less.
[0062]
[Example 2]
A lotion was prepared using the cell-active substance-containing water obtained in Example 1.
1,3-butylene glycol (humectant) ... 6.0 parts by weight
Glycerin (humectant) ... 4.0 parts by weight
Oleyl alcohol (emollient) ... 0.1 parts by weight
POE (20) sorbitan monolaurate
(Surfactant) ... 0.5 parts by weight
POE (15) lauryl alcohol ether
(Surfactant) ... 0.5 parts by weight
Ethanol (alcohol) ... 0.1 parts by weight
Phenoxyethanol (preservative): 0.2 parts by weight
Citric acid (buffering agent) ... 0.1 parts by weight
Cell-active substance-containing water ... 88.5 parts by weight
[0063]
[Example 3]
An emulsion was prepared using the cell-active substance-containing water obtained in Example 1.
1,3-butylene glycol (humectant) ... 6.0 parts by weight
Glycerin (humectant) ... 6.0 parts by weight
PEG (4000) (humectant) ... 3.0 parts by weight
POE (20) sorbitan monolaurate
(Surfactant) ... 1.5 parts by weight
POE (5) oleyl alcohol ether
(Surfactant) ... 0.3 parts by weight
Ethanol (alcohol): 10.0 parts by weight
Fragrance ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ 0.1 parts by weight
Phenoxyethanol (preservative): 0.2 parts by weight
Citric acid (buffering agent) ... 0.1 parts by weight
Cell active substance-containing water ... 72.8 parts by weight
[0064]
[Example 4]
A cream was prepared using the cell-active substance-containing water obtained in Example 1.
Stearyl alcohol (oil) ... 6.0 parts by weight
Stearic acid (oil) ... 2.0 parts by weight
Hydrogenated lanolin (oil) ... 4.0 parts by weight
Squalane (oil) ... 9.0 parts by weight
Octyldodecanol (oil) ... 10.0 parts by weight
1,3-butylene glycol (humectant) ... 6.0 parts by weight
POE (25) cetyl alcohol ether
(Surfactant) ... 3.0 parts by weight
Glyceryl monostearate
(Surfactant) ... 2.0 parts by weight
Phenoxyethanol (preservative): 0.2 parts by weight
Cell-active substance-containing water ... 65.8 parts by weight
[0065]
[Example 5]
A gel was prepared using the cell-active substance-containing water obtained in Example 1.
Dipropylene glycol (humectant) ... 7.0 parts by weight
PEG (1500) (moisturizing agent) ... 8.0 parts by weight
Carboxyvinyl polymer (water-soluble polymer): 0.4 parts by weight
Methyl cellulose (water-soluble polymer) ... 0.2 parts by weight
POE (15) oleyl alcohol ether
(Surfactant) ... 1.0 parts by weight
Potassium hydroxide (alkaline) ... 0.1 parts by weight
Phenoxyethanol (preservative) ... 0.2 parts by weight
Cell-active substance-containing water ... 83.2 parts by weight
[0066]
[Example 6]
A whitening essence was prepared using the cell-active substance-containing water obtained in Example 1.
Dipropylene glycol (humectant) ... 5.0 parts by weight
PEG (400) (humectant) ... 8 parts by weight
Ethanol (alcohol): 10.0 parts by weight
Potassium hydroxide (alkali) ... 0.15 parts by weight
Carboxyvinyl polymer (water-soluble polymer) ... 0.3 parts by weight
Sodium alginate (water-soluble polymer) ... 0.3 parts by weight
POE sorbitan monostearate
(Surfactant) ... 1.0 parts by weight
Sorbitan monooleate
(Surfactant) ... 0.5 parts by weight
Oleyl alcohol (emollient) 0.5 parts by weight
Placenta extract (drug) ... 0.2 parts by weight
Vitamin E acetate (drug) ... 0.1 parts by weight
Phenoxyethanol (preservative) ... 0.2 parts by weight
Cell-active substance-containing water ... 73.75 parts by weight
[0067]
[Example 7]
Using the cell-active substance-containing water obtained in Example 1, an ultraviolet prevention essence was prepared.
Stearic acid (oil) ... 3.0 parts by weight
Cetanol (oil) ... 1.0 parts by weight
Lanolin derivative (oil) ... 3.0 parts by weight
Liquid paraffin (oil) ... 5.0 parts by weight
2-ethylhexyl stearate (oil) ... 3.0 parts by weight
1,3-butylene glycol (humectant) ... 6.0 parts by weight
POE cetyl alcohol ether
(Surfactant) ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ 2.0 parts by weight
Glyceryl monostearate
(Surfactant) ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ 2.0 parts by weight
Triethanolamine (alkali) ... 1.0 parts by weight
2-hydroxy-4-methoxybenzophenone
(UV absorber) ... 4.0 parts by weight
Dibenzoylmethane (ultraviolet absorber) ... 4.0 parts by weight
Phenoxyethanol (preservative) ... 0.2 parts by weight
Cell-active substance-containing water ... 65.8 parts by weight
[0068]
[Example 8]
A cosmetic oil was prepared using the cell-active substance-containing water obtained in Example 1.
Olive oil (oil) ... 49.0 parts by weight
Liquid paraffin (oil) ... 30.0 parts by weight
Squalane (oil) 20.0 parts by weight
Vitamin E acetate (drug) ...... 0.2 parts by weight
Cell active substance-containing water ... 0.8 parts by weight
[0069]
[Example 9]
A pack composition was prepared using the cell-active substance-containing water obtained in Example 1.
Polyvinyl alcohol (film agent) ... 15.0 parts by weight
Carboxymethylcellulose (thickening agent): 5.0 parts by weight
1,3-butylene glycol (humectant) ... 5.0 parts by weight
Ethanol (alcohol): 12.0 parts by weight
POE oleyl alcohol ether
(Surfactant) ... 0.5 parts by weight
Phenoxyethanol (preservative) ... 0.2 parts by weight
Cell active substance-containing water ... 62.3 parts by weight
[0070]
[Example 10]
A shampoo composition was prepared using the cell-active substance-containing water obtained in Example 1.
Lauryl polyoxyethylene (3) sulfate sodium salt (30% aqueous solution)
(Surfactant) ... 30.0 parts by weight
Lauryl sulfate sodium salt (30% aqueous solution)
(Surfactant) ... 10.0 parts by weight
Isoethanolamine diethanolamine
(Surfactant) ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ 4.0 parts by weight
Glycerin (humectant) ... 1.0 part by weight
Perfume ... 0.1 parts by weight
Phenoxyethanol (preservative): 0.2 parts by weight
Cell-active substance-containing water ... 84.7 parts by weight
[0071]
Example 11
A conditioning shampoo composition was prepared using the cell-active substance-containing water obtained in Example 1.
Lauryl polyoxyethylene (3) sulfate triethanolamine salt (30% aqueous solution) (surfactant) ... 10.0 Parts by weight
Lauryl polyoxyethylene (3) sulfate sodium salt (30% aqueous solution)
(Surfactant) ... 20.0 parts by weight
Lauryl sulfate sodium salt (30% aqueous solution)
(Surfactant) ... 5.0 parts by weight
Lauryl diethanolamide
(Surfactant) ... 3.0 parts by weight
Lauryl diethanolamide
(Surfactant) ... 3.0 parts by weight
Lauryldimethylaminoacetic acid betaine
(Surfactant) ... 7.0 parts by weight
Cationized cellulose ... 0.2 parts by weight
Ethylene glycol distearate ... 2.0 parts by weight
Protein derivatives ... 0.5 parts by weight
Fragrance ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ 0.1 parts by weight
Phenoxyethanol (preservative) ... 0.2 parts by weight
Cell-active substance-containing water ... 49.0 parts by weight
[0072]
Example 12
A rinse was prepared using the cell-active substance-containing water obtained in Example 1.
Silicone oil ... 3.0 parts by weight
Liquid paraffin ... 1.0 parts by weight
Cetyl alcohol ... 1.5 parts by weight
Stearyl alcohol: 1.0 part by weight
Stearyltrimethylammonium chloride
・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ 0.7 parts by weight
Glycerin ... 3.0 parts by weight
Fragrance ... 0.1 parts by weight
Phenoxyethanol (preservative) ... 0.2 parts by weight
Cell-active substance-containing water: 89.5 parts by weight
[0073]
Example 13
A hair restorer was prepared using the cell-active substance-containing water obtained in Example 1.
Ethyl alcohol ... 60.0 parts by weight
Hinokitiol ... 0.1 parts by weight
Assembly extract ... 3.0 parts by weight
Vitamin B ₆ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ 0.1 parts by weight
Propylene glycol ... 2.0 parts by weight
Fragrance ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ 0.1 parts by weight
Phenoxyethanol (preservative) ... 0.2 parts by weight
Cell-active substance-containing water ... 34.5 parts by weight
[0074]
Example 14
A hair liquid was prepared using the cell-active substance-containing water obtained in Example 1.
Polyoxypropylene (40) butyl ether 20.0 parts by weight
Polyoxyethylene hydrogenated castor oil ... 1.0 parts by weight
Ethyl alcohol ... 50.0 parts by weight
Phenoxyethanol (preservative): 0.2 parts by weight
Cell-active substance-containing water ... 28.8 parts by weight
[0075]
Example 15
Liquid toothpaste was prepared using the cell-active substance-containing water obtained in Example 1.
Silicic anhydride ... 10.0 parts by weight
Propylene glycol ... 2.0 parts by weight
Glycerin ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ 5.0 parts by weight
Sorbit solution ... 10.0 parts by weight
Sodium polyacrylate ... 1.0 parts by weight
Sodium lauryl sulfate ... 1.0 parts by weight
Sodium saccharin ... 0.1 parts by weight
Perfume ... 0.1 parts by weight
Ethyl paraoxybenzoate 0.01 parts by weight
Cell-active substance-containing water ... 79.79 parts by weight
[0076]
Example 16
A mouthwash was prepared using the cell-active substance-containing water obtained in Example 1.
Ethanol ... 15.0 parts by weight
Glycerin ... 10.0 parts by weight
Polyoxyethylene hydrogenated castor oil: 2.0 parts by weight
Sodium saccharin ... 0.15 parts by weight
Sodium dihydrogen phosphate ... 0.1 parts by weight
Perfume ... 0.1 parts by weight
Sodium benzoate ... 0.05 parts by weight
Cell active substance-containing water ... 72.6 parts by weight
[0077]
[Example 17]
Using the cell-active substance-containing water obtained in Example 1, a wet tissue cosmetic liquid was prepared.
Methylparaben ... 0.20 parts by weight
Ethylparaben ... 0.05 parts by weight
Cetylpyridinium chloride: 0.05 parts by weight
1,3-Butylene glycol …… 5.00 parts by weight
Propylene glycol ... 5.00 parts by weight
Dipotassium glycyrrhizinate ... 0.01 parts by weight
Cell-active substance-containing water ... 89.69 parts by weight
[0078]
Example 18
A pregnancy line prevention cream was prepared using the cell-active substance-containing water obtained in Example 1.
Squalane ... 3.0 parts by weight
Olive oil ... 4.0 parts by weight
Jojoba oil ... 3.0 parts by weight
Cholesteryl isostearate: 0.5 parts by weight
Tri (capryl / capric acid) glycerin ... 3.0 parts by weight
Behenyl alcohol ... 4.0 parts by weight
Stearic acid ... 2.0 parts by weight
Self-emulsifying glyceryl monostearate ... 2.5 parts by weight
Sorbitan monostearate ... 2.5 parts by weight
Butyl paraoxybenzoate ... 0.1 parts by weight
Stearyl glycyrrhetinate 0.05 parts by weight
Methylpolysiloxane ... 0.5 parts by weight
Concentrated glycerin: 10.0 parts by weight
Sorbitol solution ... 10.0 parts by weight
N-stearoyl-sodium L-glutamate. 0.5 part by weight
Rhizoma extract ... 0.02 parts by weight
Methyl paraoxybenzoate ... 0.3 parts by weight
Cell-active substance-containing water ... 52.83 parts by weight
Horsetail extract ... 1.0 parts by weight
Button / Cuckon / Yukinoshita Extract Mixture 0.1 parts by weight
Sodium hyaluronate aqueous solution ... 0.1 parts by weight
[Brief description of the drawings]
FIG. 1 is a process diagram showing an example of a process for obtaining cellular active substance-containing water used in the present invention.
FIG. 2 is a diagram showing an example of peaks appearing in each fraction obtained by dividing the cellular active substance in the cellular active substance-containing water used in the present invention into each fraction using gel filtration chromatography. It is.

Claims (10)

A cosmetic containing the active ingredient and water,
The water is a cell-active substance-containing water composed of concentrated desalted deep-sea water that has been subjected to an operation of separating 90% by weight or more of the inorganic salt dissolved in the deep-sea water, and is contained in the cell-active substance-containing water. Cosmetics characterized in that the total amount of organic substances is in the range of 0.1 to 10% by weight . A cosmetic containing the active ingredient and water, water is concentrated and desalted deep seawater which has undergone an operation for separating at least a portion of the inorganic salts dissolved in the deep seawater, operations to further concentrate The cosmetic according to claim 1, wherein the cosmetic is water containing a cellular active substance that can be obtained by performing at least once . The above cosmetics are lotion, milky lotion, cream, gel, whitening essence, UV protection essence, cosmetic oil, pack, shampoo, conditioning shampoo, rinse, hair restorer, hair liquid, liquid toothpaste, mouthwash, wet nap chemical. The cosmetic according to claim 1 or 2, wherein the cosmetic is any one selected from the group consisting of a pregnancy line preventing cream, a body tightening gel, and a bust massage gel . The cosmetic according to claim 1 or 2, wherein the cell active substance-containing water contains a skin cell active substance and / or an immune cell active substance . The operation of the cell active substance-containing water further concentrate the concentrated and desalted deep seawater, the first term claims, characterized in that it is capable obtained by passing through two or more times or cosmetic composition of paragraph 2, wherein . The cosmetic according to claim 1 or 2, wherein the cell active substance-containing water is separated using a charged mosaic membrane. Upper Symbol concentrated and desalted deep seawater, after the deep-sea water was filtered purified, deep sea water this being filtered purified initially from 1 to 50 parts by volume relative to the amount 100 parts by volume of deep sea water used The cosmetic according to claim 1 or 2, wherein the cosmetic is obtained by concentrating to a range and separating the inorganic salt . The cellular active substance is contained in highly concentrated desalted deep sea water concentrated in the range of 0.0001 to 50.0 parts by volume when the volume of deep sea water used first is 100 parts by volume. The cosmetic material according to claim 1 or 2, wherein the cosmetic material is a cosmetic material . The cell-active substance is a first fraction having a molecular weight of 1 million or more by UV detection at a wavelength of 280 nm by gel filtration chromatography analysis using phosphate buffered saline (PBS-) as a developing solvent, and a molecular weight of 30,000 to 1,000,000. The first fraction has a peak in at least one fraction consisting of a third fraction having a molecular weight of 5000 to 30,000 and a fourth fraction having a molecular weight of 5000 or less. Or cosmetics according to item 2 . The cosmetic residue according to any one of claims 1 to 9, wherein the residue of ignition of highly concentrated desalted deep ocean water containing the cellular active substance is 0.1 wt% or less. Fee.

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