JP4663255B2 - Antibacterial and antifungal composition and method for producing antibacterial and antifungal composition - Google Patents

Description

  The present invention relates to an antibacterial and antifungal composition and a method for producing an antibacterial and antifungal composition.

  Benzoic acid and its salts, which are synthetic preservatives, are used as synthetic preservatives for foods and the like because they have a bacteriostatic action and a growth-preventing action against spoilage and deterioration. Synthetic preservatives used in foods have their use standards set by the Food Sanitation Law to ensure safety, and there are thorough standards for safety. There is a remarkable movement away from preservatives, and accordingly, additives derived from natural products for the purpose of preservation and improvement of shelf life are demanded.

  As a kind of preservatives derived from natural products, the wormwood extract containing capilin described in Non-Patent Document 1 and Patent Document 1 is known. Capillin is a compound having the following structural formula.

“Additional List of Items Listed in Existing Additives List”, 1999, p. 162 JP 2003-265152 A

  Non-Patent Document 1 discloses that the Artemisia extract has antibacterial and antifungal properties, and the active ingredient of antibacterial and antifungal properties is capilin.

  On the other hand, Patent Document 1 discloses a capilin-containing extract from which components having a specific odor are removed by extracting a capilin-containing extract from pearl millet and further distilling the extract under reduced pressure under high vacuum. Since this extract contains capilin, it exhibits antibacterial and antifungal properties as a food preservative.

  In order for the antibacterial and antifungal compound to fully exhibit its antibacterial and antifungal action, it is necessary that the concentration of the antibacterial and antifungal compound be equal to or higher than the minimum growth inhibitory concentration (MIC value). On the other hand, when the concentration of the antibacterial and antifungal composition is less than the MIC value, the higher the concentration, the higher the antibacterial and antifungal activity. This is no exception for Capilin. That is, it is a condition for exhibiting good antibacterial and antifungal properties that capilin is stably present in the antibacterial and antifungal target without decreasing.

  According to the capilin-containing extract disclosed in Patent Document 1, no measures are taken to stabilize capilin. Therefore, when capilin-containing extract is used by adding it to an antibacterial and anti-epileptic object, the amount of capilin is reduced. Will do. When bacteria and the like are mixed into the antibacterial and antiepileptic object after the reduction of capilin, the antibacterial and antiepileptic action is reduced because the concentration of capilin is reduced. As a result, the period for maintaining antibacterial and antifungal properties is not sufficient. In order to prolong the period of the antibacterial and antifungal properties of capillin, it may be possible to solve the problem by increasing the content of capillin in the antibacterial and antifungal target, but the high cost by increasing the amount of preservatives used Will be accompanied by deterioration of the flavor of food and food.

  In view of the circumstances as described above, the present invention provides an antibacterial having improved stability of capillin by suppressing a decrease in capillin itself when an antibacterial antifungal composition containing capilin is added to an antibacterial antifungal target. It is an object of the present invention to provide an antifungal composition and a method for producing an antibacterial antifungal composition suitable for the production of this composition.

  The present inventor has found that when a specific compound and capilin are combined and used as an antibacterial and antifungal composition, the stability of capillin is improved, and the present invention has been completed.

  The present invention is an antibacterial and antifungal composition characterized in that it contains a capilin solution in which capilin is dissolved in a triglyceride of a linear fatty acid and glycerin.

  The present invention also includes a capilin dissolved product obtained by dissolving capilin in a triglyceride of linear fatty acid and glycerin, a polyhydric alcohol, and a surfactant of HLB 8-20, wherein the triglyceride is 5 to 50 parts by weight, 1 to 50 parts by weight of a surfactant having an HLB value of 8 to 20 and 1 to 25 parts by weight of the polyhydric alcohol. This composition has a fatty acid glyceride, a surfactant having a HLB value of 8 to 20 and a polyhydric alcohol in a weight ratio of fatty acid glyceride: surfactant having a HLB value of 8 to 20: polyhydric alcohol = 5 to 50: 1. -50: As long as it becomes polyhydric alcohol 1-25, what was diluted with solvents, such as water, is included.

  In the above composition, the capilin may be a single substance, or may be a capilin-containing extract extracted from sagebrush.

The number of carbon atoms of the linear fatty acids, Ru 8-12 der.

Further, preferably contains a polyglycerol fatty acid ester le as a surfactant. This surfactant is preferably a surfactant having a saponification value in the range of 35 to 140.

  The polyhydric alcohol is preferably glycerin.

  The composition is preferably used in foods, and particularly preferably used as an antibacterial and antifungal composition for beverages.

In addition, the present invention is a capilin dissolving step for dissolving capilin in a fatty acid glyceride having 8 to 12 carbon atoms, and the capilin dissolving step, and a surfactant having a HLB value of 8 to 20 and a polyhydric alcohol. It is a manufacturing method of the antibacterial antifungal composition characterized by providing the emulsifier preparation process to mix, and the process of mixing the thing obtained at the said both processes.

  According to the invention of the antibacterial and antifungal composition configured as described above, even when this composition is used as an antibacterial and antifungal object for antibacterial and antifungal applications, the stability of capillin is improved. As a result, even if bacteria or sputum is mixed in the container containing the antibacterial and antifungal object, the amount of capillin that exists stably is large, so the period of antibacterial and antifungal effects can be prolonged. it can. Furthermore, even when the antibacterial antiepileptic composition of the above configuration is stored before use, it improves the stability of capillin in the antibacterial antiepileptic composition, and the decrease in the capillin is suppressed. The storage period of the antibacterial antiepileptic composition can be extended.

  Moreover, according to the method for producing an antibacterial and antifungal composition configured as described above, an antibacterial and antifungal composition in which capilin is stably present can be produced.

  Hereinafter, the present invention will be described in detail based on embodiments.

  The antibacterial and antifungal composition according to the present invention is an antibacterial and antifungal composition containing capilin and a fatty acid glyceride. This antibacterial antiepileptic composition is produced by mixing capilin and fatty acid triglycerides.

  It is recommended to use the capilin contained in the mugwort. In this case, it is good to use the extract of Kawara mugwort containing a capilin. The extract of Kawamomogi is obtained by immersing the Artemisia in a solvent, removing the Artemisia from the solvent, and then distilling off the removed solvent. Moreover, after extracting Kawara mugwort in water vapor | steam, an extract of Kawara mugwort can be obtained by collecting this water vapor | steam.

  As for the wormwood used for obtaining the extract of the wormwood, it is preferable to use the above-ground part of the cultivated wormwood, and it is preferable to use dried ears.

  As a solvent for immersing the mugwort, organic solvents such as monohydric or polyhydric alcohols, ketones, ethers, hydrocarbons and water may be used alone or in combination. Examples of the monohydric alcohol include methanol, ethanol, and isopropyl alcohol. Examples of the polyhydric alcohol include ethylene glycol, propylene glycol, 1,3-butylene glycol, and glycerin. Examples of ketones include acetone and methyl ethyl ketone, examples of ethers include methyl ether and petroleum ether, and examples of hydrocarbons include hexane and cyclohexane. Preferably, ethanol or hydrous ethanol is used.

  In order to obtain the extract of Kawara mugwort, this solvent can be distilled off from the solvent in which the mugwort is soaked. A component containing capyline is appropriately separated by liquid chromatography using a developing solvent having a boiling point, and then the developing solvent is evaporated by heating and evaporating. When the solvent is evaporated by heating, it is usually performed under reduced pressure conditions. In addition, when the solvent used in order to obtain the extract of Kawara mugwort does not interfere with the use as the antibacterial antifungal composition according to the present invention, the solvent may not be evaporated off.

  In addition, in order to increase the concentration of capillin in the extract of Kawara mugwort, water was further added to the extract of extract of mugwort, and this water was distilled off, followed by liquid-liquid extraction with the extract of Kawara mugwort and n-hexane. It is good to carry out one or both operations of collecting the subsequent n-hexane and distilling off the n-hexane.

  Fatty acid glycerides are esters of glycerin and fatty acids, and fatty acid glycerides containing one or more of monoglycerides, diglycerides and triglycerides are used. In addition, when fatty acid glyceride is solid, it is good to melt | dissolve in the fatty-acid glyceride which melt | dissolved capyrin after heating this.

  Fatty acid glyceride is manufactured by the method of esterifying a well-known fatty acid and glycerol, for example. Fatty acids used for producing fatty acid glycerides are butyric acid, caprylic acid, pelargonic acid, capric acid, undecyl acid, lauric acid, lauric acid, myristic acid, palmitic acid, palmitoleic acid, stearic acid, oleic acid, linoleic acid, Linolenic acid, noctinic acid, alginic acid and the like are used, and one or more of these fatty acids may be selected and used.

  Moreover, you may use the natural fats and oils which contain a triglyceride as a main component for a fatty acid glyceride. As the natural fats and oils, animal fats and animal oils that are animal oils, or vegetable fats and oils such as vegetable fats and vegetable oils may be used.

  Examples of animal fats include milk fat, goat milk fat, beef fat, pork fat, and sheep fat. Examples of animal oils include sardine oil, mackerel oil, and shark liver oil. Examples of vegetable fats include palm oil and palm oil. As the vegetable oil, it is possible to use drying oil, semi-drying oil and non-drying oil. Examples of the drying oil include linseed oil, tung oil, safflower oil, and examples of the semi-drying oil include soybean oil, Examples include corn oil, sesame oil, rapeseed oil, sunflower oil, and cottonseed oil. Examples of non-drying oil include olive oil, mustard oil, camellia oil, castor oil, and peanut oil. Moreover, the constituent fats and oils contained in the natural fats and oils can be separated and used.

  As the fatty acid glyceride, it is preferable to use a fatty acid glyceride obtained by esterifying one or two or more fatty acids having 8 to 12 carbon atoms and glycerin. In addition, when the antibacterial and antifungal composition according to the present invention is used in foods such as beverages, in order to give food consumers the same taste as foods that do not contain the antibacterial and antifungal composition according to the present invention, It is preferable to use fatty acid triglycerides, and it is particularly preferable to use tri (caprylic / capric) glycerides.

  The antibacterial and antifungal composition containing capilin, a fatty acid glyceride, a surfactant and a polyhydric alcohol, which is another antibacterial and antifungal composition according to the present invention, is preferably capilin that dissolves capilin in a fatty acid glyceride. It is manufactured by separately performing a dissolution step and an emulsifier preparation step of mixing a surfactant and a polyhydric alcohol, and mixing the products obtained in both steps. It is possible to use the antibacterial antifungal composition prepared by emulsifying and dispersing it in an aqueous solvent as the antibacterial antifungal composition according to the present invention. It is preferable to emulsify and disperse by adding. The method of emulsifying and dispersing in this way is a suitable method for preparing an emulsion having improved transparency.

  The capilin dissolution step is performed with stirring so that the capilin is dissolved in the fatty acid glyceride. If necessary, stirring may be performed with heating. At this time, the mixing ratio of capillin and fatty acid glyceride is not particularly limited as long as capillin is dissolved in fatty acid glyceride, but the fatty acid glyceride is preferably 10 to 1000 parts by weight with respect to 1 part by weight of capilin. In this step, the capilin is preferably contained in the Artemisia, similar to the capilin used in the antibacterial and antifungal composition containing the above-mentioned capilin and fatty acid glyceride. Even in this case, it is preferable to use an extract of Capsicum containing capilin. In addition, the fatty acid glyceride in this step may be the fatty acid glyceride. Suitable fatty acid glycerides are the same as the fatty acid glycerides used in the antibacterial and antiepileptic composition containing the capillin and fatty acid glycerides.

In the emulsifier preparation step, the emulsifier is prepared by stirring and mixing the surfactant and the polyhydric alcohol. This prepared emulsifier enables the capilin solution prepared in the capilin dissolution step to become an oil-in-water emulsion in an aqueous solvent. The mixing of the surfactant and the polyhydric alcohol is preferably performed so that the surfactant is 1 to 50 parts by weight and the polyhydric alcohol is 1 to 25 parts by weight.

  As the surfactant used in the emulsifier preparation process, a surfactant having an HLB value (Hydrophile-Lipophile Balance Value) calculated from Griffin's empirical formula of 8 to 20, preferably 14 to 20 is selected and used. Good. This surfactant is a monoglyceride such as citric acid monoglyceride, diacetyltartaric acid monoglyceride, glycerin monocaprylate, diglycerin monocaprylate, diglycerin monomyristate, diglycerin monopalmitate, diglycerin monostearate, diglycerin monooleate. Polyglycerin fatty acid monoesters such as acid, decaglycerin monocaprylate, decaglycerin monolaurate, decaglycerin monomyristate, decaglycerin monostearate, decaglycerin monoaleate, sucrose monolaurate, sorbitan fatty acid ester, etc. Examples include sugar fatty acid esters, polyglycerin condensed ricinoleic acid esters, and polyoxyethylene sorbitan fatty acid esters. Among these, decaglycerin monomyristate is preferable.

  The glyceride, polyglycerin fatty acid ester, and sugar fatty acid ester used in the surfactant are, for example, glycerin, polyglycerin, or sugar, and fatty acid using an alkali catalyst such as sodium hydroxide, and a reaction temperature of 160 to 260 ° C. It is produced by esterification under a nitrogen gas atmosphere. When the monoester is produced, the glyceride, polyglycerin fatty acid ester, and sugar fatty acid ester are esterified so that the charged molar ratio of glycerin, polyglycerin, or sugar and fatty acid is 1: 1. The saponification value of the surfactant produced by esterification is preferably in the range of 35 to 140.

  Examples of the polyhydric alcohol include glycerin, ethylene glycol, propylene glycol, 1,3-butylene glycol, and 1,2-pentanediol. When the composition according to the present invention is used for food, glycerin and propylene glycol which are acceptable as food additives in the Food Sanitation Law are used. In order to obtain a fine emulsion, it is preferable to select glycerin.

  The antimicrobial and antifungal composition according to the present invention is produced by mixing the capilin dissolved product obtained in the capilin dissolving step and the emulsifier obtained in the emulsifier preparing step. In this case, it is preferable to mix the capilin solution with the emulsifier. In this mixing, the concentration of the capyline lysate is not particularly limited, but it is preferable to mix so that the capilin in the capyline lysate is contained in the antibacterial and antifungal composition at 0.0001 to 50% by weight. The concentration of the other components of the antibacterial and antifungal composition is 5 to 50% by weight, preferably 5 to 25% by weight, more preferably 5 to 10% by weight, and the surfactant. Is 1 to 50% by weight, preferably 1 to 25% by weight, more preferably 1 to 8% by weight, and polyhydric alcohol is 1 to 25% by weight, preferably 1 to 10% by weight, more preferably 1%. ~ 5% by weight. In addition, it is also possible to dilute and use this antibacterial and antifungal composition in a solvent such as water. At this time, the weight ratio of the fatty acid glyceride, the surfactant having an HLB value of 8 to 20 and the polyhydric alcohol is , Fatty acid glyceride: surfactant having a HLB value of 8 to 20: polyhydric alcohol = 5 to 50: 1 to 50: 1 to 25 is preferable.

  Moreover, even if it is a composition containing a surfactant with capillin and HLB of 8 to 20, it exhibits antibacterial and antifungal effects. In this composition, as the capillin and the surfactant, the capillin and the surfactant may be used. Moreover, it is preferable to use the suitable capilin and to use the suitable surfactant. In this composition as well, as in the antibacterial and antifungal composition, capilin is stabilized. This composition is made by mixing capilin and a surfactant.

  The antibacterial and antifungal composition can be used for beverages, confectionery, soy sauce, miso, noodles, dairy products, sugar beet, agricultural products such as fruits and vegetables, and foods such as fish and shellfish. Preferably, it is used for confectionery, soy sauce, miso, and beverages.

  Capillin, fatty acid glyceride 5 to 50 parts by weight, antibacterial and antifungal composition containing 1 to 50 parts by weight of surfactant having an HLB value of 8 to 20 and 1 to 25 parts by weight of polyhydric alcohol are sufficiently dispersed in the beverage And it does not cause turbidity in the beverage. This composition is particularly suitable for use in beverages contained in containers that can be freely opened and closed.

  The antibacterial and antifungal composition can be used without limitation for cosmetics, various cleaning agents, inks, paints, and the like.

  When the antibacterial and antifungal composition is used, it can be used by adding the composition to the antibacterial and antifungal object, and when it cannot be added, it is applied or sprayed on the surface of the antibacterial and antifungal object. Can be used. Further, after the antibacterial and antifungal composition is dispersed in a solvent such as water, this solvent may be used as the antibacterial and antifungal composition.

  The antibacterial and antifungal composition according to the present invention is a genus Asperugillus genus such as Penicillium paxilli, Penicillium digitatum (green wilt fungus), Penicillium italicum, etc. Thermoscus genus which is a thermophilic moth, Acremonium genus which is a hygroscopic moth, Eurotium genus such as Eurotium herbariorum which is a drought moth It exhibits an antibacterial and antifungal effect especially against yeasts of the genus Candida such as albicans (candida yeast) and the genus Saccharomyces such as Saccharomyces cerevisiae (beer and bread yeast). That is, the antibacterial and antifungal composition according to the present invention exhibits an antibacterial and antifungal effect particularly against fungi.

  In addition, the antibacterial and antifungal composition according to the present invention is also effective against bacteria such as Staphylococcus aureus (P. aureus), Pseudomonas aeruginosa (Pseudomonas aeruginosa), Escherichia coli (E. coli), and Propionibacterium (Acne bacteria). Demonstrate. In this case, it is preferable to use other antibacterial and antifungal compositions in combination as necessary. Antibacterial and antifungal compositions to be used in combination include organic acid salts such as sodium acetate, ethanol, glycine, polylysine, protamine, lysozyme, chitosan, pectin degradation products, plant extracts such as Soso bamboo, and medium chain fatty acids such as caprylic acid monoglyceride. Substances having an antibacterial action may be used in combination with bacteria such as these esters.

  Further, the antibacterial and antifungal composition according to the present invention includes sorbic acid and a salt thereof, benzoic acid and a salt thereof, dehydroacetic acid and a salt thereof, isopropylmethylphenol, paraoxybenzoic acid esters, phenoxyethanol, benzalkonium chloride, chloride Select one or more types of preservatives, bactericides and fungicides of various chemical synthesis systems such as alkyltrimethylammonium, chlorhexidine gluconate, triclosan, thiabendador, benzimidazole, siabendazole, etc. Also good.

EXAMPLES Hereinafter, although this invention is shown concretely based on an Example, this invention is not limited to these.

(Preparation of Kawaramugi extract)
500 g of dried Kawamomogi ears were immersed in 2500 g of ethanol at 37 ° C. for 17 hours. After immersion, the filtrate obtained by filtering ethanol was concentrated under the conditions of 420 mmHg and 70 to 75 ° C., and then 1000 g of ion-exchanged water was added to the remaining concentrated solution and distilled under the conditions of 250 to 400 mmHg and 70 to 80 ° C. Went. This distillation operation was performed a total of 8 times. Liquid-liquid extraction was performed by adding 500 ml of n-hexane to the fraction after distillation. This extraction was repeated three times. After extraction, the n-hexane extract was concentrated under the conditions of 460 mmHg and 60 ° C. to obtain a Kawaramugi extract. The capilin content in the extract of Kawaramugi was 20% by weight. The capillin content in the extract of Kawamomogi was quantified by analysis by high performance liquid chromatography.

The antibacterial and antifungal compositions of the examples and the compositions of the comparative examples were prepared as follows.
(Comparative Example 1a )
75 g of ethanol was added to 0.5 g of Kawaramugi extract, and then 10 g of decaglycerin monomyristate having an HLB value of 15 and a saponification value of 55 was prepared to prepare a mixed solution. Thereafter, ethanol was distilled off under reduced pressure to obtain a concentrated solution. 89.5 g of water was added to this concentrated solution to prepare an antibacterial and antifungal composition of Comparative Example 1a .

( Comparative Example 1b )
Prepare capyline lysate by mixing 0.5 g of Kawara mugwort extract and 20 g of olive oil. Separately, prepare 10 g of decaglycerin monomyristate having an HLB value of 15 and a saponification value of 55 and 10 g of glycerin to prepare an emulsifier. did. These were mixed by adding and stirring the capyline dissolved material in the emulsifier, and further adding and stirring 59.5 g of water to prepare the antibacterial and antifungal composition of Comparative Example 1b .

(Example 3)
A capyline lysate is prepared by mixing 0.5 g of Kawara mugwort extract extract and 20 g of tri (caprylic / capric acid) glyceryl (Nisshin Oilio Co., Ltd. “ODO”), and has an HLB value of 15, An emulsifier was prepared by mixing 10 g of decaglycerin monomyristate having a saponification value of 55 and 10 g of glycerin. These were mixed by adding and stirring the capillin solution in the emulsifier, and further adding 59.5 g of water and stirring to prepare the antibacterial and antifungal composition of Example 3.

Example 4
A capillin solution is prepared by mixing 0.5 g of Kawara mugwort extract and 20 g of tri (caprylic / capric acid) glyceryl (Nisshin Oilio Co., Ltd. “ODO”), and then adding 79.5 g of water. And the antibacterial and antifungal composition of Example 4 was prepared by stirring.

(Example 5)
An antibacterial and antifungal composition of Example 5 was prepared by mixing Kawara mugwort extract and tri (capryl / capric acid) glyceryl (“ODO” manufactured by Nisshin Oilio Co., Ltd.). At this time, the amount of the extract of Kawara mugwort was such that the concentration of capillin in the antibacterial and antifungal composition was about 1000 ppm.

(Example 6)
An antibacterial and antifungal composition of Example 6 having a capillin concentration of about 500 ppm was prepared. This composition was prepared in the same manner as in Example 5.

(Comparative Example 2)
The antibacterial and antifungal composition of Comparative Example 2 was prepared by stirring and mixing 0.5 g of Kawara mugwort extract and 99.5 g of water.

  The stability of capillin was confirmed based on the following Test Examples 1-1, 1-2 and 1-3.

[Test Example 1-1]
An antibacterial antiepileptic composition mixed solution was prepared by mixing with water so that the antibacterial and antifungal compositions of Example 3 and Comparative Examples 1a, 1b, and 2 would be about 0.1% by weight. 50 ml of this antibacterial and antifungal composition mixed solution was injected into a 50 ml screw tube, the screw tube was sealed, and then left in a dark room at 37 ° C. for 14 days. The stability of capillin was confirmed by measuring the change with time in the capillin concentration before and after the standing.

[Test Example 1-2]
An antibacterial and antifungal composition mixture was prepared by mixing with an orange juice beverage so that the antibacterial and antifungal compositions of Example 3 and Comparative Examples 1a, 1b, and 2 would be about 0.1% by weight. The other conditions were the same as in Test Example 1-1, and the stability of capillin was confirmed. The composition of the orange juice drink is 110 g of fructose-glucose liquid sugar, 200 g of orange juice, 1.5 g of citric acid, 1 g of fragrance, 0.5 g of vitamin C, and the remaining weight is 1000 g using water. did. This orange juice drink had a pH of 3.5.

[Test Example 1-3]
An antibacterial and antifungal composition mixture was prepared by mixing with an apple juice beverage so that the antibacterial and antifungal compositions of Example 3 and Comparative Examples 1a, 1b, and 2 would be about 0.1% by weight. The other conditions were the same as in Test Example 1-1, and the stability of capillin was confirmed. The composition of the apple juice drink is 110 g of fructose glucose liquid sugar, 200 g of apple fruit juice, 1.5 g of citric acid, 1 g of fragrance, 0.5 g of vitamin C, and the remaining weight is 1000 g using water. did. The apple juice drink had a pH of 3.5.

  Table 1 shows the test results of Test Examples 1-1, 1-2, and 1-3. FIG. 1 is a graph comparing the capillin residual ratios of Examples and Comparative Examples in Table 1. In Table 1, the concentration is the concentration of capillin, and the residual rate is a value calculated from the residual rate (%) = the concentration after 14 days / initial concentration × 100. The concentration is a concentration determined by high performance liquid chromatography.

As can be seen from Table 1, the remaining ratio of capillin is higher in the order of Example 3, Comparative Example 1b , Comparative Example 1a , and Comparative Example 2 regardless of Test Examples 1-1, 1-2, and 1-3. It has become. From this, it can be confirmed that the composition of Comparative Example 1a containing decaglycerin monomyristate is more stable than that of Comparative Example 2. The comparison in comparison with Example 1a, olive oil or tri Kapirin of Comparative Example 1b and Example 3 contains a (caprylic-capric acid) glyceryl and glycerol can be confirmed to be stable. That is, the composition containing a surfactant having an HLB value of 15 improves the stability of capillin, and the composition containing a fatty acid glyceride and a polyhydric alcohol further improves the stability of capillin. That is confirmed.

An antibacterial and antifungal confirmation test for the antibacterial and antifungal composition of Example 3 was performed. This confirmation test was performed by adding yeast after leaving the composition of Example 3 or Comparative Example 2 to stand. The composition is allowed to stand by injecting 500 ml of a beverage into a polyethylene terephthalate (PET) bottle, adding a predetermined amount of the antibacterial and antifungal composition of Example 3 or Comparative Example 2 to the injected beverage, The opening was closed and left at 50 ° C. for 14 days. Before and after this standing, the concentration of capillin in the beverage was measured. The yeast was added by inoculating Saccharomyces cerevisiae NBRC0216 at a concentration of about 1.45 × 10 ³ cfu / mL in the beverage after standing, and closing the opening of the PET bottle. In addition, growth suppression of yeast was confirmed by measuring the number of viable bacteria after a predetermined number of days.

Beverages used for the antibacterial and anti-bacterial confirmation test are as follows.
[Test Example 2-1] An orange juice beverage having the same composition as Test Example 1-2 was used.
[Test Example 2-2] An apple juice drink having the same composition as Test Example 1-3 was used.

  Table 2 shows the results of the antibacterial and antifungal confirmation test. In Table 2, the concentration is a value quantified by high performance liquid chromatography. The remaining rate is a value calculated in the same manner as described above.

As can be seen from Table 2, in Test Example 2-1, the capillin remaining rate of Example 3 is generally higher than that of Comparative Example 2, and the stability of capillin is high.

  In Table 2, Table 3 is a table in which the viable cell counts of Test Example 2-1, Example 3 having an initial capillin concentration of 0.46 ppm and Comparative Example 2 having 0.95 ppm were directly compared.

In Table 3, the capillin concentrations after 14 days of Example 3 and Comparative Example 2 are comparable. The viable cell counts of Example 3 and Comparative Example 2 having the same concentration are not significantly different after 3 days and 7 days, and the antibacterial and antifungal composition of Example 3 according to the present invention is an antibacterial agent of capyrin It can be confirmed that the antibacterial effect is exhibited without inhibiting the antidepressant effect.

  Moreover, in Table 2, in Test Example 2-2, it can be confirmed that the capillin residual rate of Example 3 is higher than that of Comparative Example 2 and that Capillin is stable, as in Test Example 2-1. In addition, the number of viable bacteria depends on the concentration after 14 days of capillin, and the composition of Example 3 which is a composition according to the present invention exhibits antibacterial and antifungal effects without inhibiting the antibacterial and antifungal effects of capilin. It can be confirmed that

The antibacterial and antifungal properties of the compositions of Examples 3 to 4 and Comparative Examples 1a, 1b, and 2 were tested based on the following Test Examples 3-1 and 3-2.

[Test Example 3-1] Any of the compositions of Examples 3 to 4 and Comparative Examples 1a, 1b, and 2 was added to strawberry jam. The amount of this composition added to strawberry jam was 0.1% by weight. 20 g of strawberry jam to which any of the compositions of Examples 3 to 4 and Comparative Examples 1a, 1b and 2 was added was collected in a sterilized petri dish. After the opening of the petri dish was sealed, it was left for 14 days at 50 ° C. Next, after opening the petri dish, the petri dish was left under conditions of 25 ° C. for 40 days. The state of occurrence of wrinkles during this standing period was visually observed every 5 days.

[Test Example 3-2] Any one of the compositions of Examples 3 to 4 and Comparative Examples 1a, 1b, and 2 was added to white beans. The amount of this composition added to white bean was added to be 0.1% by weight. 20 g of white beans to which any of the compositions of Examples 3 to 4 and Comparative Examples 1a, 1b, and 2 were added were collected in a sterilized petri dish. After the opening of the petri dish was sealed, it was left for 14 days at 50 ° C. Next, after opening the petri dish, the petri dish was left under conditions of 25 ° C. for 7 days. The state of occurrence of soot during this standing period was visually observed every other day.

  Table 4 shows the test results of Test Examples 3-1 and 3-2. Table 4 shows the date when the occurrence of wrinkles was confirmed.

It can be confirmed that the strawberry jam to which the composition of the example was added has a longer period when the occurrence of wrinkles was confirmed than the strawberry jam to which the composition of Comparative Example 2 was added. The same applies to white bean paste.

  Using the compositions of Example 5 and Example 6, a test for confirming the stability of capillin was conducted. This test was performed based on the following Test Example 4.

[Test Example 4]
50 ml of the composition of the example was injected into a 50 ml screw tube, and the screw tube was sealed. Thereafter, the screw tube was left in a dark room at a room temperature of 50 ° C. for 28 days. Capillin stability was tested by measuring the capillin concentration after 14 and 28 days.

  The results of Test Example 4 are shown in Table 5. In Table 5, the residual ratio is a value calculated from the residual ratio (%) = concentration after standing / initial concentration × 100. The concentration is the concentration determined by high performance liquid chromatography.

As shown in Table 5, the capillin concentration in the compositions of Example 5 and Example 6 hardly fluctuated. That is, it can be confirmed that capillin in the composition containing capilin and fatty acid glyceride is stable.

It is the graph which compared the capillin residual rate of the Example in Table 1, and a comparative example.

Claims (6)

1. An antibacterial and antifungal composition comprising a capyline dissolved product in which capilin is dissolved in a triglyceride of a linear fatty acid having 8 to 12 carbon atoms and glycerin. Antibacterial and antifungal composition of claim 1 containing polyglycerol fatty acid ester le as a surfactant. Containing a capilin solution in which capilin is dissolved in a triglyceride of a linear fatty acid having 8 to 12 carbon atoms and glycerin, a polyhydric alcohol, and a surfactant of HLB 8-20,
5 to 50 parts by weight of the triglyceride, 1 to 50 parts by weight of the surfactant having an HLB value of 8 to 20, and 1 to 25 parts by weight of the polyhydric alcohol. . The antibacterial and antifungal composition according to any one of claims 1 to 3 , wherein the antibacterial and antifungal composition is used for food. The antibacterial and antifungal composition according to claim 4 , wherein the food is a beverage. A capilin dissolving step of dissolving capilin in a triglyceride of linear fatty acid having 8 to 12 carbon atoms and glycerin;
An emulsifier preparation step which is performed separately from the capilin dissolution step and which mixes a surfactant having a HLB value of 8 to 20 and a polyhydric alcohol;
A method for producing an antibacterial and antifungal composition, comprising the step of mixing the products obtained in both steps.