JP4856017B2 - Biosensor - Google Patents

Description

  The present invention relates to a biosensor capable of quantitative analysis by measuring the amount of a substrate component in a specimen.

  Conventionally, biosensors have been devised for measuring blood sugar levels and the like of specimens (see, for example, Patent Document 1 and Patent Document 2). According to this biosensor, the blood glucose level or the like can be measured by measuring the current value between the working electrode and the counter electrode when the reaction part reacts with blood or the like introduced from the supply port.

Japanese Patent Publication No. 08-01208 International Publication No. 2004/017057 Pamphlet

  When measuring a blood glucose level, etc., using a biosensor, the biosensor is inserted into the sensor mounting opening of the measurement display. However, since the biosensor is a consumable item, it is removed from the measurement display after measurement. To be disposed of.

  Here, since the work of removing the biosensor from the measurement display after the measurement was performed while holding the biosensor by a human hand, there is a risk of suffering from an infection from blood adhering to the biosensor. The removal work was done with great care, such as wearing gloves.

  In addition, with biosensors that have been downsized due to recent technological developments, there has been a further increase in the risk of blood adhering to the biosensor adhering to the hand, as well as the attachment and removal of biosensors from measurement displays. Workability and handling are also getting worse. Especially for elderly people and those who are not good at fine fingertips, it is extremely difficult to attach a small biosensor to a measurement display or to remove a biosensor without blood on the hand. .

  In view of the above problems, the inventor of the present application can easily perform attachment and detachment work to and from the measurement display, and blood and the like attached to the biosensor adhere to the hand during the detachment work. As a result of intensive studies to provide a biosensor that is difficult to perform, the present invention has been achieved.

That is, the biosensor of the present invention is a biosensor that is attached to a measurement display and measures the amount of a substrate component in a specimen,
A substrate made of an insulator and an insulator, which is integrally formed by extending from the substrate, and has a support substrate edge portion, and an insulator, which is integrally formed by extending from the substrate and the support substrate. A gripping substrate,
The substrate, a working electrode provided on the substrate, a counter electrode provided at a certain distance from the working electrode, the working electrode, a reaction part provided on the counter electrode, and a specimen to the reaction part A sensor unit including a supply port to be introduced, the support substrate, a support unit side working electrode extending from the working electrode on the support substrate, and a predetermined interval from the support unit side working electrode; A support portion provided with a support portion side counter electrode extending from the counter electrode, and a gripping portion provided with the gripping substrate and gripped .
The sensor unit is disposed in a state of protruding from the support unit, and the gripping unit is disposed in a state of surrounding the sensor unit in the protruding direction of the sensor unit from the support unit, and measures the amount of the substrate component for, when the bending the gripping portion at the boundary portion between the grip portion and the support portion, the supply port is protruded from the supporting portion, in order to remove from the measuring indicator grips the gripping portion The sensor part including the supply port is surrounded by the gripping part when the bent gripping part is returned to the original state .

  According to the biosensor of the present invention, when measuring the amount of the substrate component in the specimen, the supply port provided in the sensor unit is in a state of protruding from the support unit by bending the grip substrate provided in the grip unit toward the support substrate. The support substrate and the gripping substrate do not hinder measurement work, and it is possible to easily spot blood or the like on the supply port. On the other hand, after the measurement, since the sensor unit including the supply port is surrounded by the gripping unit, when the biosensor is removed from the measurement display, it is possible to prevent adhesion of blood or the like adhering to the sensor unit to the hand. Therefore, it is possible to prevent infection from blood adhering to the biosensor.

  In addition, the biosensor of the present invention can easily adhere to the hand of blood, prevent infection, etc. due to its structure, so that the efficiency of measurement work in the medical field and the like, improvement in handling of the biosensor, etc. Is also planned.

  Furthermore, the biosensor of the present invention is configured in such a manner that the small sensor unit is integrated with the support unit, and the support unit and the grip unit are integrated, so that the biosensor is attached to the measurement display. In addition, the handling work and the workability of the measurement work can be improved especially for elderly people and people who are not good at fine work with fingertips.

  Furthermore, in the biosensor of the present invention, a boundary portion is provided at a position crossing the sensor portion, and the grip portion can be easily and reliably bent at a predetermined position by bending the grip portion at the boundary portion. When measuring the substrate component amount, the supply port can be easily and reliably projected from the support portion.

  Hereinafter, embodiments of the biosensor of the present invention will be described with reference to the drawings. The biosensor 10 according to the present embodiment shown in FIGS. 1A and 1B is a biosensor that measures the amount of a substrate component in a specimen, and includes a sensor unit 12, a support unit 14, and a grip unit 15. It is configured to include.

  The sensor unit 12 includes an insulating substrate 16, a working electrode 18 provided on the substrate 16, a counter electrode 20, a working electrode 18, and a counter electrode provided on the substrate 16 and spaced apart from the working electrode 18. 20 and a supply port 24 for introducing a specimen such as blood or urine to the reaction unit 22. The reaction unit 22 reacts to the sample introduced from the supply port 24. The amount of the substrate component in the sample (for example, blood glucose level, etc.) can be measured.

  The support unit 14 is made of an insulator and is integrated with the substrate 16 in the sensor unit 12, and on the support substrate 28, the edge of the support substrate 28 from the working electrode 18 in the sensor unit 12 (= support substrate edge). The support portion side working electrode 30 extended to the portion 28a) and the support substrate 28 are spaced from the support portion side working electrode 30 by a certain distance from the counter electrode 20 in the sensor portion 12 to the support substrate edge portion 28a. The support part side counter electrode 32 provided is provided.

  The grip portion 15 includes a grip substrate 26 made of an insulator and integrated with the support substrate 28 in the support portion 14. In addition, in the biosensor 10 of this embodiment, the gripping substrate 26 is integrated with the substrate 16 before the formation of the sensor unit 12. However, when the sensor unit 12 is formed, the sensor unit 12 and the gripping unit are integrated. By cutting along the boundary line with the substrate 15, the substrate 16 and the gripping substrate 26 are formed.

  Reference numeral 33 denotes a boundary portion between the support portion 14 and the grip portion 15, that is, a boundary portion between the support substrate 28 provided in the support portion 14 and the grip substrate 26 provided in the grip portion 15. As shown in FIG. 4, it becomes a bent portion when the grip portion 15 is bent toward the support portion 14 side. The effect of the boundary portion 33 will be described later.

  The insulator constituting the substrate 16, the support substrate 28 and the gripping substrate 26 is, for example, a polyester resin sheet such as polyethylene terephthalate (PET), polyethylene naphthalate, a biodegradable polyester resin composed of an aliphatic unit and an aromatic unit, Examples thereof include a polyamideimide sheet and a polyimide sheet such as a polyimide sheet which are more excellent in heat resistance, chemical resistance and strength, and an inorganic substrate such as a ceramic.

  The working electrode 18, the counter electrode 20, the support portion side working electrode 30, and the support portion side counter electrode 32 each have a band shape, and the working electrode 18 and the counter electrode 20 are formed on the substrate 16 and the support portion side working electrode 30 and The support portion side counter electrodes 32 are arranged in parallel on the support substrate 28 at a predetermined interval. The working electrode 18 and the like are formed on the substrate 16 or the support substrate 28 by a good electric conductor such as platinum, gold, nickel, palladium, or indium-tin oxide. As a forming method, hot stamping can be considered. Vacuum deposition or sputtering is preferable because a fine electrode pattern can be formed with high accuracy. In the case of sputtering, it can be formed all at once by masking the outside of electrode formation.

  The reaction unit 22 includes an oxidoreductase and an electron acceptor. For example, it is configured by applying and drying a liquid material containing an oxidoreductase and an electron acceptor. Examples of the oxidoreductase include glucose oxidase when measuring glucose. Glucose oxidase reacts with glucose to produce gluconic acid and hydrogen peroxide. Moreover, when measuring an alcohol value, alcohol oxidase or alcohol dehydrogenase is used. When measuring lactic acid, lactate oxidase or lactate dehydrogenase is used. When measuring uric acid, uricase is used. Generally, it is an inorganic or organic fine powdery compound that promotes oxidation-reduction of enzymes. For example, alkali metal ferricyanide (particularly potassium ferricyanide metal salt is preferable), ferrocene or an alkyl-substituted product thereof, p-benzoquinone, methylene blue, potassium β-naphthoquinone-4-sulfonate, phenazine methosulfate, 2,6- Examples include dichlorophenol-indophenol. Ferricyanide alkali metal salts and ferrocenes have a stable function as an electron transfer medium and are well soluble in aqueous solvents such as water, alcohols, or mixed solvents thereof, and thus effectively function as electron acceptors.

  As shown in FIGS. 1B and 2, the supply port 24 is formed by the leading edge 36 a of the cover 36 provided on the substrate 16 via the spacer 34 and the leading edge 16 a of the substrate 16. . That is, the supply port 24 is formed by a gap generated by the spacer 34 between the leading edge 36a and the leading edge 16a. A sample such as blood introduced from the supply port 24 can reach the leading edge 34a of the spacer 34 by capillary action if the amount is sufficient.

  In the biosensor 10 of the present embodiment, the substrate 16 of the sensor unit 12, the support substrate 28 of the support unit 14, and the grip substrate 26 of the grip unit 15 are integrated as shown in FIG. Then, the substrate 16 of the sensor unit 12 and the gripping substrate 26 of the gripping unit 15 are formed by cutting along the boundary line 17 between the sensor unit 12 and the gripping unit 15. That is, at this stage, the substrate 16 of the sensor unit 12 and the support substrate 28 of the support unit 14 are integrated, and the support substrate 28 and the grip substrate 26 of the grip unit 15 are integrated. The working electrode 18 and the counter electrode 20 are provided on the substrate 16 in the sensor unit 12 at a predetermined interval, and the working electrode 18 and the counter electrode provided in the sensor unit 12 are provided on the support substrate 28 in the support unit 14. The support portion side working electrode 30 and the support portion side counter electrode 32 are extended from the support substrate edge portion 28a to the support substrate edge portion 28a at regular intervals. And as shown in FIG.3 (b), the sensor part 12 and the support part 14 apply | coat the reaction part 22 on the working electrode 18 and the counter electrode 20, and it covers with the cover 36 via the spacer 34. The sensor unit 12 is formed in a state where the sensor unit 12 protrudes from the support unit 14, and the grip unit 15 is disposed so as to surround the sensor unit 12 in the protruding direction of the sensor unit 12 from the support unit 14. The biosensor 10 of the embodiment is formed.

  The biosensor 10 of the present embodiment configured as described above is inserted into a sensor mounting opening provided in a measurement display (not shown) when measuring the amount of a substrate component such as a blood glucose level. Below, the effect | action at the time of measuring a blood glucose level using this biosensor 10 is demonstrated.

  The blood glucose level measurer first inserts the support substrate edge portion 28a side of the support portion 14 of the biosensor 10 into a sensor mounting opening of a measurement display (not shown). Next, the gripping substrate 26 of the gripping part 15 that is integral with the support substrate 28 of the support part 14 is connected to the boundary part 33 between the support substrate 28 and the gripping substrate 26, in other words, the boundary between the support part 14 and the gripping part 15. As shown in FIG. 4, the supply port 24 in the sensor unit 12 is protruded from the support unit 14 by being bent at the unit 33. Specifically, the edge portion of the gripping substrate 26 opposite to the boundary portion 33, that is, the tip end portion 29 side of the gripping substrate 26 is bent at the boundary portion 33 toward the support substrate edge portion 28 a side. Since the supply port 24 in the sensor unit 12 is disposed so as to protrude from the support unit 14, the grip unit 15 is bent toward the support unit 14 at the boundary 33, thereby protruding from the boundary 33 toward the grip 15. In addition, the supply port 24 surrounded by the gripping part 15 protrudes from the support part 14. In addition, the bending direction of the holding part 15 is not specifically limited, You may bend | fold on the opposite side to the direction shown in figure.

  In the state where the supply port 24 of the sensor unit 12 is protruded from the support unit 14 as described above, blood that has been pierced with a finger or the like is spotted on the supply port 24. The blood spotted on the supply port 24 flows through the gap between the substrate 16 and the cover 36 along the working electrode 18 and the counter electrode 20 toward the tip edge 34a of the spacer 34 by capillary action. Then, an enzymatic reaction is performed in the reaction unit 22, and the blood glucose level is measured by detecting the electrochemical change at that time with the working electrode 18 and the counter electrode 20, and the measurement result is displayed on the measurement display.

  After the blood glucose level is measured, the grip 15 that has been bent at the boundary 33 is returned to its original state. And a series of blood glucose level measurement operation | movement is complete | finished by holding | gripping the holding | grip part 15 returned to the original and removing the biosensor 10 from the sensor attachment opening part of a measurement indicator.

  As described above, according to the biosensor 10 of the present embodiment, when measuring the amount of a substrate component such as a blood glucose level, the supply port 24 protrudes from the support portion 14, so that the support portion 14 and the grip portion 15 are measured. The work is not hindered, and blood or the like can be easily spotted on the supply port 24 as in the conventional biosensor. On the other hand, since the sensor unit 12 including the supply port 24 is surrounded by the gripping unit 15 after the measurement, when the biosensor 10 is removed from the measurement display, it prevents adhesion of blood or the like adhering to the sensor unit 12 to the hand. obtain. Therefore, it is possible to prevent infection from blood adhering to the sensor unit 12.

  In addition, because of the structure of the biosensor 10, blood can be easily attached to the hand and infections can be prevented more easily than conventional biosensors. The improvement of handling is also possible.

  Furthermore, the biosensor 10 of the present embodiment is configured in such a manner that the small sensor unit 12 is integrated with the support unit 14 and the support unit 14 and the gripping unit 15 are integrated with each other. Can be easily attached to and removed from the measurement display, especially for elderly people and those who are not good at fine work with the fingertips. Workability and the like can be improved.

  The biosensor 10 according to the embodiment of the present invention exemplified above should not be construed as substantially limiting the technical idea of the present invention. For example, the position of the boundary portion 33 is not limited to the positions shown in FIGS. As described above, the boundary portion in the present invention is a bent portion for bending the grip portion toward the support portion. Therefore, as long as the boundary portion 33a shown in FIG. 5A is provided in a direction crossing the sensor portion 12, it may be formed obliquely with respect to the longitudinal direction of the support portion 14. Even when the grip portion 15 is bent toward the support portion 14 by the boundary portion 33a, the supply port 24 of the sensor portion 12 protrudes from the support portion 14, and blood or the like to the supply port 24 is measured when measuring the amount of the substrate component. This is not hindered by the support substrate 28 or the gripping substrate 26.

  Further, in the above-described embodiment, the boundary portions 33 and 33a are formed with cuts in advance so that the grip portion 15 can be easily bent at a predetermined position. However, the biosensor of the present invention is shown in FIG. ) Like the biosensors 10a and 10b shown in (c), an embodiment may be provided that includes a gripping portion that is easily bent at a predetermined position without providing a notch at the boundary portion. In the biosensor 10a shown in FIG. 5B, the side edges 43 and 43 ′ (= the side edges of the gripping substrate 42) of the support substrate 40 where the boundary portion 41 between the support portion 14a and the gripping portion 15a is located. Recesses 44 and 44 'are respectively provided. Therefore, when trying to bend the gripping portion 15a, a bending portion connecting the recesses 44 and 44 'provided in the side edge portions 43 and 43' is induced, and as a result, the gripping portion 15a becomes the boundary portion. The supply port 24 can be protruded from the support portion 14a.

  The biosensor 10b shown in FIG. 5C is configured to include a support portion 14b having a quadrangular support substrate 46 and a grip portion 15b having a substantially circular grip substrate 48. The supply port 24 protrudes from the support portion 14b and is positioned closer to the gripping portion 15b than the boundary portion 47 between the support portion 14b and the gripping portion 15b. Therefore, when the grip portion 15b is to be bent, the bent portion is induced at the boundary portion 47, and as a result, the supply port 24 can be protruded from the support portion 14b.

  The biosensors 10, 10a, and 10b according to the embodiments of the present invention exemplified above should not be construed as substantially limiting the technical idea of the present invention. For example, the shape of the front view formed by the support portion and the grip portion is not limited to a square shape such as the biosensor 10 or 10a, but a combination of a square and a circle such as the support portion 14b and the grip portion 15b in the biosensor 10b. The shape may be any shape such as a square shape, an elliptical shape, a sector shape, or a combination of other polygonal shapes. Further, like the biosensor 10c shown in FIG. 6, the support substrate 50 in the support portion 14c and the grip substrate 52 in the grip portion 15c may be integrated in a bent state, or may be curved. It is also possible to form a single unit.

  In addition, the shape of the working electrode 18 and the counter electrode 20 in the sensor unit 12, the number of electrodes, and the like are not particularly limited, and the present invention is appropriately improved and changed by the ingenuity and ingenuity of those skilled in the art without departing from the scope of the invention. Or it can be implemented while adding.

(A) is a front view of the biosensor which concerns on one Embodiment of this invention, (b) is A-A 'sectional drawing in (a). It is an enlarged front view of the sensor part in FIG. It is explanatory drawing of the assembly method of the biosensor shown in FIG. 1, (a) is a front view before providing the reaction part, (b) is a front view which shows the state which provides a spacer and a cover. It is sectional drawing which shows the state which bent the biosensor shown in FIG. 1 in the boundary part. (A)-(c) is a front view which shows the biosensor which concerns on other embodiment of this invention. It is a front view which shows the biosensor which concerns on other embodiment of this invention.

Explanation of symbols

10, 10a to 10c: biosensor 12: sensor unit 14, 14a to 14c: support unit 15, 15a to 15c: gripping unit 16: substrate 18: working electrode 20: counter electrode 22: reaction unit 24: supply port 26, 42 48, 52: Grasping substrate 28, 40, 46, 50: Support substrate 30: Support portion side working electrode 32: Support portion side counter electrode 33, 33a, 41, 47: Boundary portion

Claims (1)

A biosensor attached to a measurement display for measuring the amount of a substrate component in a specimen,
A substrate made of an insulator;
A support substrate made of an insulator, integrally formed extending from the substrate, and having a support substrate edge;
A gripping substrate made of an insulator and extending integrally from the substrate and the support substrate; and
With
The substrate, a working electrode provided on the substrate, a counter electrode provided at a certain distance from the working electrode, the working electrode, a reaction part provided on the counter electrode, and a specimen to the reaction part A sensor unit including a supply port to be introduced;
The support substrate, a support portion side working electrode extending from the working electrode on the support substrate, and a support portion side facing extending from the counter electrode with a certain distance from the support portion working electrode. A support portion comprising an electrode;
A gripping portion to be gripped with the gripping substrate ; and
Comprising
The sensor part is disposed in a state of protruding from the support part,
The gripping part is disposed in a state of surrounding the sensor part in the protruding direction of the sensor part from the support part,
To measure the substrate component amount, upon bending the gripping portion at the boundary portion between the grip portion and the support portion, said supply port protrudes from the support portion,
In order to grip the gripping part and remove it from the measurement display, when the gripping part that has been bent is returned to its original state, the sensor part including the supply port is surrounded by the gripping part. Biosensor.

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