JP4869979B2 - Coffee bean extract-containing aqueous composition, container-packed beverage, method for producing coffee bean extract-containing aqueous composition, and method for preventing precipitation of coffee bean extract - Google Patents

Description

  The present invention relates to a coffee bean extract-containing aqueous composition, a packaged beverage, a method for producing a coffee bean extract-containing aqueous composition, and a method for preventing precipitation of a coffee bean extract.

  Coffee bean extract, especially raw coffee bean extract, has various effects on the human body, such as antihypertensive effect, fecal improvement effect, diabetes prevention effect, anti-obesity effect, antioxidant effect, etc. by ingestion. It is shown that there is. From the viewpoint of improving absorbability when ingested, it is desirable to blend the coffee bean extract into a beverage. When an effective amount of coffee bean extract is blended with these coffee bean extract-containing beverages, coffee beans are extracted in beverages, especially acidic beverages, when stored at low temperatures or under conditions of repeated low and high temperatures. There was a problem that precipitation derived from the product occurred. In particular, when blending vitamin Bs, particularly vitamin B1, in the beverage, it is preferable to make the beverage acidic from the viewpoint of the stability of vitamin Bs, and when blending the coffee bean extract and vitamin Bs simultaneously, This problem was remarkable.

In order to prevent the precipitation of the coffee bean extract in the coffee bean extract-containing beverage, for example, Patent Document 1 discloses a pH of 5.0 to 6.5 containing a sucrose fatty acid ester having a monoester content of 90% by weight or more. A coffee beverage is disclosed. Patent Document 2 discloses a weakly acidic black coffee beverage characterized by containing a seed polysaccharide such as locust bean gum and a fermented polysaccharide such as xanthan gum. Patent Document 3 discloses a method of treating with an enzyme treatment of a filamentous fungus having a galactomannanase and acidic protease activity.
Japanese Patent Laid-Open No. 10-70956 JP 2001-120184 A JP 2002-272375 A

However, during long-term storage for more than 3 months even at low temperatures, or during long-term storage under unstable temperature conditions that cause temperature changes during such long-term storage, precipitation may occur if the stability is poor. Occurs.
The present invention provides a coffee bean extract-containing aqueous composition having excellent storage stability even at low temperatures or under unstable temperature conditions, a method for producing the same, a packaged beverage, and a method for preventing precipitation of a coffee bean extract. For the purpose.

The coffee bean extract-containing aqueous composition of the present invention contains a coffee bean extract and oil-in-water emulsion particles containing carotenoids.
The container-packed beverage of the present invention contains a coffee bean extract and oil-in-water emulsion particles containing carotenoids.
The method for producing a coffee bean extract-containing aqueous composition of the present invention includes blending a liquid component containing a coffee bean extract and an oil-in-water emulsion composition containing carotenoids.
The method for preventing precipitation of a coffee bean extract according to the present invention includes blending an oil-in-water emulsion composition containing carotenoids with an aqueous composition containing a coffee bean extract.

Here, the carotenoids are preferably astaxanthins.
Moreover, it is preferable that the particle diameter of the said emulsion is 200 nm or less.
It is preferable that the coffee bean extract-containing aqueous composition further contains ascorbic acids and / or vitamin Bs.
The coffee bean extract may be a green coffee bean extract.

  According to the present invention, a coffee bean extract-containing aqueous composition having excellent storage stability even at low temperatures and unstable temperature conditions, a method for producing the same, a packaged beverage, and a method for preventing precipitation of a coffee bean extract Can be provided.

The coffee bean extract-containing aqueous composition of the present invention contains a coffee bean extract and oil-in-water emulsion particles containing carotenoids.
In the present coffee bean extract-containing aqueous composition, since the coffee bean extract is dispersed in the aqueous composition together with the oil-in-water emulsion particles containing carotenoids, it is good even under low temperature and unstable temperature conditions. Long-term storage stability.
The container-packed beverage of the present invention contains a coffee bean extract and oil-in-water emulsion particles containing carotenoids.
In this container-packed beverage, the coffee bean extract in the beverage is dispersed in the beverage together with the oil-in-water emulsion particles containing carotenoids, as in the case of the aqueous composition containing the coffee bean extract. Good long-term storage stability can be exhibited even under moderate temperature conditions.
The present invention will be described below.

<Coffee bean extract>
The coffee bean extract in the present invention is an extract from coffee beans of coffee tree fruit, and as the coffee beans, for example, any varieties such as Arabica, Robusta, Riberica and Arabsta are used. The production area is not particularly limited.
The coffee beans used in the coffee bean extract used in the present invention may be either green beans or roasted beans, and green beans are particularly preferable.
The extraction method for obtaining the coffee bean extract is not particularly limited. The raw coffee beans may be unground or ground, and may be subjected to pretreatment such as impurity removal as long as the quality of the extract can be maintained.

The coffee bean extract may be a liquid obtained by filtering the extract, a post-processed liquid such as decolorized, or a concentrated liquid obtained by concentrating it. Further, a powder obtained by spray-drying or freeze-drying this extract may be used.
Examples of the extraction solvent include water, a hydrophilic organic solvent, and a supercritical fluid. Examples of the hydrophilic organic solvent include methanol, ethanol, 2-propanol, acetone, methyl ethyl ketone, and the like. The hydrophilic organic solvent is preferable, and hydrous ethanol is particularly preferable.
The extraction conditions are not particularly limited, but normal temperature or heat extraction is preferable. The heating temperature and the heating time can also be set to various conditions as long as extraction can be sufficiently performed and the quality of the extract can be maintained.

  The content of the coffee bean extract in the aqueous composition is preferably 100 mg or more, more preferably 200 mg or more, and particularly preferably 300 mg or more, as a solid content in 100 ml of the entire aqueous composition. If it is less than 100 mg, the effectiveness of the coffee beans on the human body is difficult to obtain, and precipitation may not be a problem.

<Emulsion particles>
In the present invention, the emulsion particles contain carotenoids. Here, the oil-in-water emulsion particles in the present invention mean oil droplets in the oil-in-water emulsion.
[Carotenoids]
In the present invention, by using carotenoids (also called carotenoids) which are oil-soluble functional pigments, the dispersion stability and storage stability of oil droplets in the present aqueous composition can be improved.

The carotenoids in the present invention are preferably carotenoids containing natural pigments, which are yellow to red terpenoid pigments, including those of plants, algae, and bacteria.
Moreover, it is not limited to the thing of natural origin, Any thing will be contained in the carotenoid in this invention if it can be obtained in accordance with a conventional method. For example, many of the carotenoids of carotenoids described below are also produced by synthesis, and most of commercially available β-carotene is produced by synthesis.

Examples of carotenoids include hydrocarbons (carotenes) and oxidized alcohol derivatives thereof (xanthophylls).
Examples of these include actinioerythrol, astaxanthin, bixin, canthaxanthin, capsanthin, capsorbin, β-8′-apo-carotenal (apocarotenal), β-12′-apo-carotenal, α-carotene, β-carotene, "Carotene" (mixture of α- and β-carotenes), γ-carotene, β-cryptoxanthin, echinone, lutein, lycopene, biorelythrin, zeaxanthin, and esters of those containing hydroxyl or carboxyl .

Many of the carotenoids occur naturally in the form of cis and trans isomers, but the composites are often racemic mixtures.
Carotenoids can generally be extracted from plant materials. These carotenoids have a variety of functions, for example, lutein extracted from marigold petals is widely used as a raw material for poultry food, coloring the poultry skin and fat and eggs produced by poultry There is.

The carotenoids used in the present invention are preferably oily at normal temperature from the viewpoint of reducing the emulsion particle size. Particularly preferred examples include derivatives such as astaxanthin and esters of astaxanthin that have an antioxidant effect, an anti-inflammatory effect, an anti-skin aging effect, a whitening effect, etc., and are known as colorants in the yellow to red range ( Hereinafter, at least one selected from “astaxanthins”) may be included.
These astaxanthins are more preferably extracted from natural materials using supercritical carbon dioxide in terms of odor when powdered.

Astaxanthin is a red pigment with absorption maxima at 476 nm (ethanol) and 468 nm (hexane) and belongs to a kind of carotenoid xanthophyll (Davies, BH: In “Chemistry and Biochemistry of Plant Pigments”, TW Goodwin ed., 2nd. ed., 38-165, Academic Press, NY, 1976.). The chemical structure of astaxanthin is 3,3′-dihydroxy-β, β-carotene- ₄ , 4′-dione (C ₄ OH ₅₂ O ₄ , molecular weight 596.82).

  Astaxanthin has a 3S, 3S′-form, 3S, 3R′-form (meso-form), 3R, 3R ′-, due to the configuration of the 3 (3 ′)-positioned hydroxyl groups of the ring structure present at both ends of the molecule. There are three isomers of the body. In addition, there are cis- and trans- isomers of conjugated double bonds at the center of the molecule. For example, all cis-, 9-cis and 13-cis isomers.

  The hydroxyl group at the 3 (3 ')-position can form an ester with a fatty acid. Astaxanthin obtained from krill is a diester (Yamaguchi, K., Miki, W., Toriu, N., Kondo, Y., Murakami, M., Konosu, S., Satake, M., Fujita). , T .: The composition of carotenoid pigments in the antarctic krill Euphausia superba, Bull. Jap. Sos. Sci. Fish., 1983, 49, p.1411-1415. The product obtained from Pluvialis is 3S, 3S'-, which contains many monoesters with one fatty acid (Renstrom, B., Liaaen-Jensen, S .: Fatty acids of some esterified carotenols, Comp. Biochem Physiol. B, Comp. Biochem., 1981, 69, p. 625-627.).

  Astaxanthin obtained from Phaffia Rhodozyma is a 3R, 3R′-form (Andrewes, AG, Starr, MP: (3R, 3′R) -Asttaxanthin from the yeast Phaffa rhodozyma, Phytochem., 1976, 15, p.1009. -1011.) And has the opposite structure to the 3S, 3S′-form normally found in nature. It also exists in free form that does not form esters with fatty acids (Andrewes, AG, Phaffia, HJ, Starr, MP: Carotenids of Phaffia rhodozyma, a red pigmented fermenting yeast, Phytochem., 1976, 15, p. .1003-1007.)

  Astaxanthin and its ester are R.I. Kuhn et al. First isolated from lobster (Astacus gammarus L.) and disclosed its putative structure (Kuhn, R., Soerensen, NA: The coloring matters of the lobster (Astacus gammarus L.), Z. Angew. Chem. , 1938, 51, p.465-466.). Since then, it has been clarified that astaxanthin is widely distributed in nature and usually exists as an astaxanthin fatty acid ester, and also exists as an astaxanthin protein (oborbin, cluster cyanine) bound to proteins in crustaceans (Cheesman , DF: Ovorubin, a chromoprotein from the eggs of the gastropod mollusc Pomacea canaliculata, Proc. Roy. Soc. B, 1958, 149, p.571-587.).

The astaxanthin and its ester (astaxanthins) may be contained in the powder composition of the present invention as an astaxanthin-containing oil separated and extracted from a natural product containing astaxanthin and / or its ester. Examples of such astaxanthin-containing oils include red yeast faffia, green algae hematococcus, marine bacteria, and the like, and extracts from the culture, extracts from Antarctic krill, and the like.
It is known that a haematococcus alga extract (haematococcus alga-derived pigment) differs from a krill-derived pigment or synthesized astaxanthin in terms of the type of ester and its content.

  Astaxanthins that can be used in the present invention may be the above-mentioned extract (extract extract), a product obtained by appropriately purifying the extract as necessary, or a synthetic product. As the astaxanthins, those extracted from Haematococcus alga (hereinafter also referred to as Haematococcus alga extract) are particularly preferred from the viewpoint of quality and productivity.

Specific examples of the haematococcus alga extract that can be used in the present invention include Haematococcus pluviaris, Haematococcus lacustris, Examples thereof include Haematococcus droebakensis, Haematococcus zimbabiensis, and the like.
The method for culturing Haematococcus algae that can be used in the present invention can employ various methods disclosed in JP-A-8-103288 and the like, and is not particularly limited. What is necessary is just to change the form to a cyst cell.

The Haematococcus alga extract that can be used in the present invention is prepared by using the above-mentioned raw materials, if necessary, by crushing the cell wall by a method disclosed in, for example, JP-A-5-68585, etc., and adding acetone, ether, chloroform, and alcohol. It can be obtained by adding an organic solvent such as (ethanol, methanol, etc.) or an extraction solvent such as supercritical carbon dioxide.
The Haematococcus alga extract contains astaxanthin or an ester thereof as the pure pigment, as in the dye described in JP-A-2-49091, and the ester is generally at least 50 mol%, preferably 75 mol. % Or more, more preferably 90 mol% or more.
In addition, in the present invention, commercially available Haematococcus alga extract can be used, for example, ASTOTS-S, -2.5O, -5O, -10O, etc., manufactured by Takeda Shiki Co., Ltd. Examples include Asteryl Oil 50F and 5F manufactured by Fuji Chemical Industry Co., Ltd., and BioAstin SCE7 manufactured by Toyo Enzyme Chemical Co., Ltd.
In the present invention, the content of pure astaxanthin in the Haematococcus alga extract is preferably 0.001 to 50% by mass, more preferably 0.01 to 25% by mass from the viewpoint of extraction cost. It is.

  In the present invention, the carotenoids are preferably 0.0001 to 0.1% by mass, more preferably 0.0005, from the viewpoint of refining the particle diameter of the oil droplets and emulsion stability with respect to the entire aqueous composition. -0.05 mass%, More preferably, it is 0.001-0.02 mass%. If it is 0.0001% by mass or more, the effect of preventing precipitation is sufficient, and if it is 0.1% by mass or less, the effect can be efficiently obtained with respect to the addition amount, which is preferable.

<Emulsion composition>
The emulsion particles according to the present invention are not particularly limited as long as they are contained in an aqueous composition, but include a liquid component containing a coffee bean extract and an oil-in-water emulsion composition containing carotenoids. It is preferable that it is contained in this aqueous composition by manufacturing the aqueous composition of this invention.
Here, when the liquid component containing the coffee bean extract and the oil-in-water emulsion composition are blended, the oil-in-water emulsion composition is 0.02% by mass to 20% by mass with respect to the entire aqueous composition. %, More preferably 0.1% by mass to 10% by mass, and still more preferably 0.2% by mass to 4.0% by mass. If it is 0.02% by mass or more, the dispersion of the coffee bean extract can be maintained while ensuring the function of the functional component, and if it is 20% by mass or less, emulsion particles can be efficiently blended in the liquid component. This is preferable because it is possible.
Such an oil-in-water emulsion composition preferably contains an oily component (containing carotenoids), a phosphorus substance, and a surfactant.

[Oil component]
The emulsion composition in the present invention can contain the carotenoids as an oily component in the oil-in-water emulsion composition. In this case, the amount of the carotenoid is preferably 0.1 to 50% by mass, more preferably 0.3 to 25% by mass, and still more preferably 0.5 to 10% by mass with respect to the emulsion composition. be able to. If it is 0.1% by mass or more, the effect of preventing precipitation is sufficient, and if it is 50% by mass or less, the effect can be obtained efficiently with respect to the addition amount, which is preferable.
In addition to the carotenoids described above, the oily component in the emulsion composition can include a component that does not dissolve in the aqueous medium but dissolves in the oily medium. For example, vitamin E (tocopherol, tocotrienol, etc.) , Ubiquinones, and omega-3 oils and fats (oils and fats containing EPA, DHA, linolenic acid, and the like).

(A) Vitamin E Vitamin E is preferable because it can bring about a synergistic effect on antioxidant power when combined with carotenoids.
Tocopherols are selected from the group of compounds consisting of tocopherols or derivatives thereof. The compound group consisting of tocopherol or its derivatives includes dl-α-tocopherol, dl-β-tocopherol, dl-γ-tocopherol, dl-δ-tocopherol, dl-α-tocopherol acetate, nicotinic acid-dl-α-tocopherol. , Linoleic acid-dl-α-tocopherol, tocopherols such as dl-α-tocopherol succinate and derivatives thereof, α-tocotrienol, β-tocotrienol, γ-tocotrienol, δ-tocotrienol and the like. These are often used in the form of a mixture, and can be used in a state called extracted tocopherol, mixed tocopherol or the like. The content of tocopherol with respect to the carotenoid in the emulsion composition of the present invention is not particularly limited, but is preferably a ratio of 0.1 to 5, more preferably 0.2 to 3, and still more preferably with respect to the carotenoid amount. The ratio is 0.5-2.

(B) Ubiquinones As preferred oily components other than carotenoid pigments, ubiquinones, particularly coenzyme Q10, can be mentioned. Coenzyme Q10 was approved and sold in Japan in 1974 as an ethical drug for metabolic cardiotonic drugs. Since then, it has been treated as a medicine including OTC. On the other hand, overseas (mainly Europe and America) has been growing in demand for health food materials with high effectiveness and safety for the past 10 years. And in Japan, in 2001, the Ministry of Health, Labor and Welfare's Director General of Pharmacy “Revision of Standards on the Scope of Drugs” (Pharmaceutical Development No. 243) stated that Coenzyme Q10 “ (Raw materials) "list has been released and the regulation has been relaxed so that it can be handled as food. In Japan, attention has been paid to the various functions of this food material, and many general foods (so-called health foods) containing coenzyme Q10 are being commercialized.

  In order to make use of the function of coenzyme Q10, it is important to make this material, which is a fat-soluble substance, water-soluble. In the present invention, since the functional oily component is protected in a good state, the particle size when the powder is redispersed in water can be kept small, and the transparency of the liquid can be improved, In addition, sufficient absorption in the living body can be achieved.

(C) ω-Oils and Fats Examples of other oil components preferred for the present invention include ω (omega) -3 oils and fats of unsaturated fatty acids having a double bond at the ω-3 position. Examples thereof include linolenic acid, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), and fish oils containing these.
Among them, DHA is an abbreviation for Docosahexaenoic acid, and is a general term for carboxylic acids having 22 carbon chains containing six double bonds (22: 6). , 7, 10, 13, 16, 19 all have cis-type double bonds.

(D) Fats and oils In addition to the above, compounds that can be used as oily components include liquid fats and oils (fatty oils) and solid fats and oils (fats) at room temperature.
Examples of the liquid oil include olive oil, camellia oil, macadamia nut oil, castor oil, avocado oil, evening primrose oil, turtle oil, corn oil, mink oil, rapeseed oil, egg yolk oil, sesame oil, persic oil, wheat germ oil, and sasanca Oil, flaxseed oil, safflower oil, cottonseed oil, eno oil, soybean oil, peanut oil, tea seed oil, kaya oil, rice bran oil, cinnagari oil, Japanese kiri oil, jojoba oil, germ oil, triglycerin, glycerin trioctanoate, Examples include glycerin triisopalmitate, salad oil, safflower oil (safflower oil), palm oil, coconut oil, peanut oil, almond oil, hazelnut oil, walnut oil, grape seed oil, squalene, and squalane.
Moreover, as the solid fats and oils, beef tallow, hardened beef tallow, beef leg fat, beef bone fat, mink oil, egg yolk oil, pork tallow, horse fat, sheep fat, hardened oil, cocoa butter, palm oil, hardened palm oil, Palm oil, palm hardened oil, owl, owl kernel oil, hardened castor oil and the like can be mentioned.
Among these, from the viewpoint of the particle size and stability of the emulsion composition, coconut oil which is a liquor triglyceride is preferably used.

  Other oily components include, for example, hydrocarbons such as liquid paraffin, paraffin, petrolatum, ceresin, microcrystalline wax, waxes such as carnauba wax, candelilla wax, jojoba oil, beeswax, lanolin, isopropyl myristate, myristic acid 2 -Esters such as octyldodecyl, cetyl 2-ethylhexanoate, diisostearyl malate, fatty acids such as palmitic acid, stearic acid, isostearic acid, linoleic acid, arachidonic acid, cetyl alcohol, stearyl alcohol, isostearyl alcohol, Examples include higher alcohols such as 2-octyldodecanol, silicone oils such as methylpolysiloxane and methylphenylpolysiloxane, other polymers, oil-soluble pigments, and oil-soluble proteins. . In addition, various plant-derived oils and animal-derived oils that are mixtures thereof are also included.

  In order to further improve the dispersibility in water, the oil component is preferably used in combination of two or more. As the oil component that can be used in combination for this purpose, DHA, squalene, and squalane are preferable, and squalene is particularly preferable. In particular, in the case of an oily component that is solid at room temperature, such as coenzyme Q10, it is particularly preferable to use it together with DHA, squalene, squalane and the like.

  The content of the oil component in the emulsion composition is preferably 0.1 to 50% by mass, more preferably 0.3 to 25% by mass, and still more preferably 0, from the viewpoints of refining the emulsion particle size and emulsion stability. 5 to 10% by mass.

[Phospholipid]
In the present invention, phospholipid is an ester composed of fatty acid, alcohol, phosphoric acid and nitrogen compound among complex lipids, and is a group having phosphate ester and fatty acid ester, and refers to glycerophospholipid, sphingophospholipid, and lecithin. Sometimes called. Details will be described below.

  Examples of the phospholipid that can be used in the present invention include lecithin, phosphatidic acid, bisphosphatidic acid, phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol (cardiolipin) and the like. Glycerolecithin, sphingolecithin such as sphingomyelin, and the like. Moreover, various lecithins derived from plants such as soybeans, corn, peanuts, rapeseed, wheat and the like, those derived from animals such as egg yolk and cows, and microorganisms such as Escherichia coli can be mentioned. The origin of these phospholipids is not particularly limited, but purified ones are particularly suitable.

  Since phospholipids have a hydrophilic group and a hydrophobic group in the molecule, they have been widely used as emulsifiers in the food, pharmaceutical and cosmetic fields. Industrially, phospholipids having a purity of 60% or more are used as lecithin and can be used in the present invention. From the viewpoint of formation of fine oil droplet diameters and stability of functional oil components, This is generally called high-purity lecithin, which has a lecithin purity of 80% by mass or more, more preferably 90% by mass or more.

In the present invention, glycerophospholipid that has been enzymatically decomposed can be used as glycerophospholipid.
For example, lysolecithin (enzymatically decomposed lecithin) obtained by enzymatic degradation of lecithin is a product in which either one of fatty acids (acyl groups) bonded to the 1-position or 2-position of glycerophospholipid is lost. By using one fatty acid group, the hydrophilicity of lecithin can be improved, and the emulsifiability and dispersibility in water can be improved.
Lysolecithin can be obtained by hydrolysis of lecithin with an acid or alkali catalyst, but can be obtained by hydrolysis of lecithin using phospholipase A ₁ or A ₂ .
When lyso compounds represented by such lysolecithin are represented by compound names, lysophosphatidic acid, lysophosphatidylglycerin, lysophosphatidylinositol, lysophosphatidylethanolamine, lysophosphatidylmethylethanolamine, lysophosphatidylcholine (lysolecithin), lysophosphatidylserine Is mentioned.

Furthermore, the glycerophospholipids typified by the above lecithin can also be used in the present invention after being hydrogenated or hydroxylated.
The hydrogenation is performed, for example, by reacting lecithin with hydrogen in the presence of a catalyst, and the unsaturated bond of the fatty acid moiety is hydrogenated. Hydrogenation improves the oxidation stability of lecithin.
Moreover, the said hydroxylation heats a lecithin with high concentration hydrogen peroxide and organic acids, such as an acetic acid, tartaric acid, and butyric acid, and the unsaturated bond of a fatty-acid part is hydroxylated. Hydroxylation improves the hydrophilicity of lecithin.
Among these, glycerophospholipids lecithin and lysolecithin are preferable from the viewpoint of emulsion stability, and lysolecithin is more preferable.

The phospholipid used in the present invention can be used alone or in the form of a mixture of plural kinds.
In the emulsion composition used in the present invention, the phospholipid content is preferably 0.1 to 10% by mass, more preferably 0.2 to 5% by mass, and still more preferably 0.3 to 2% by mass. is there.
When the content of the phospholipid is 0.1% by mass or more, the emulsion stability of the emulsion composition tends to be good. Moreover, it is preferable from the viewpoint of the emulsion stability of an emulsion composition by making the said content into 10 mass% or less, without an excess phospholipid separating from an oil-based component and forming a phospholipid dispersion in water.

[Surfactant]
As the surfactant in the present invention, a nonionic surfactant emulsifier (hydrophilic surfactant) that dissolves in an aqueous medium can greatly reduce the interfacial tension of the oil phase / water phase in the emulsion composition. As a result, it is preferable in that the particle diameter can be reduced.
Specifically, HLB of 8 or more is preferable, 10 or more is more preferable, and 12 or more is particularly preferable.
The upper limit value of the HLB value is not particularly limited, but is generally 20 or less, and preferably 18 or less.

Here, HLB is a hydrophilic-hydrophobic balance that is usually used in the field of surfactants, and a commonly used calculation formula such as the Kawakami formula can be used. In the present invention, the following Kawakami equation is adopted.
HLB = 7 + 11.7log (Mw / M0)
Here, Mw is the molecular weight of the hydrophilic group, and M0 is the molecular weight of the hydrophobic group.

Moreover, you may use the numerical value of HLB described in the catalog etc.
Further, as can be seen from the above formula, a surfactant having an arbitrary HLB value can be obtained by utilizing the additivity of HLB.

  The surfactant that can be used in the present invention is not particularly limited, but a nonionic surfactant is preferable. Examples of nonionic surfactants include glycerin fatty acid ester, organic acid monoglyceride, polyglycerin fatty acid ester, propylene glycol fatty acid ester, polyglycerin condensed ricinoleic acid ester, sorbitan fatty acid ester, sucrose fatty acid ester, polyoxyethylene sorbitan Examples include fatty acid esters. More preferred are polyglycerin fatty acid ester, sorbitan fatty acid ester, sucrose fatty acid ester, and polyoxyethylene sorbitan fatty acid ester. In addition, the surfactant described above is not necessarily highly purified by distillation or the like, and may be a reaction mixture.

The polyglycerol fatty acid ester used in the present invention has an average degree of polymerization of 2 or more, preferably 6 to 15, more preferably 8 to 10 and a fatty acid having 8 to 18 carbon atoms such as caprylic acid and capric acid. , Esters with lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid, and linoleic acid. Preferred examples of polyglycerol fatty acid esters include hexaglycerol monooleate, hexaglycerol monostearate, hexaglycerol monopalmitate, hexaglycerol monomyristate, hexaglycerol monolaurate, decaglycerol monooleate , Decaglycerin monostearic acid ester, decaglycerin monopalmitic acid ester, decaglycerin monomyristic acid ester, decaglycerin monolauric acid ester and the like.
Among these, more preferably, decaglycerol monooleate (HLB = 12), decaglycerol monostearate (HLB = 12), decaglycerol monopalmitate (HLB = 13), decaglycerol monomyristate (HLB = 14), decaglycerin monolaurate (HLB = 16), and the like.
These polyglycerin fatty acid esters can be used alone or in combination.
Examples of commercially available products include Nikko Chemicals, Inc., NIKKOL DGMS, NIKKOL DGMO-CV, NIKKOL DGMO-90V, NIKKOL DGDO, NIKKOL DGMIS, NIKKOL DGTI, NIKKOL DGTI, NIKKOL DGTI Tetraglyn 3-S, NIKKOL Tetraglyn 5-S, NIKKOL Tetraglyn 5-O, NIKKOL Hexaglyn 1-L, NIKKOL Hexaglyn 1-M, NIKKOL Hexaglyn 1-SV, NIKKOL Hexaglyn 1-O, NIKKOL Hexaglyn 3-S, NIKKOL Hexaglyn 4 -B, NIKKOL Hexaglyn 5-S, IKKOL Hexaglyn 5-O, NIKKOL Hexaglyn PR-15, NIKKOL Decaglyn 1-L, NIKKOL Decaglyn 1-M, NIKKOL Decaglyn 1-SV, NIKKOL Decaglyn 1-50SV, NIKKOL Decaglyn 1-ISV, NIKKOL Decaglyn 1-O, NIKKOL Decaglyn 1-OV, NIKKOL Decaglyn 1-LN, NIKKOL Decaglyn 2-SV, NIKKOL Decaglyn 2-ISV, NIKKOL Decaglyn 3-SV, NIKKOL Decaglyn 3-OV, NIKKOL Decaglyn 5-SV, NIKKOL Decaglyn 5-HS, NIKKOL Decaglyn 5- I , NIKKOL Decaglyn 5-OV, NIKKOL Decaglyn 5-O-R, NIKKOL Decaglyn 7-S, NIKKOL Decaglyn 7-O, NIKKOL Decaglyn 10-SV, NIKKOL Decaglyn 10-IS, NIKKOL Decaglyn 10-OV, NIKKOL Decaglyn 10-MAC , NIKKOL Decaglyn PR-20, Ryoto polyglycerase manufactured by Mitsubishi Chemical Foods, Inc., L-7D, L-10D, M-10D, P-8D, SWA-10D, SWA-15D, SWA-20D, S- 24D, S-28D, O-15D, O-50D, B-70D, B-100D, ER-60D, LOP-120DP, DS13W, DS3, HS11, HS9, TS4, S2, DL15, DO13, Sunsoft Q-17UL, Sunsoft Q-14S, Sunsoft A-141C, Riken Vitamin Co., Ltd. Poem DO-100, Poem J-0021, etc. It is done.
Among these, preferably, NIKKOL Decaglyn 1-L, NIKKOL Decaglyn 1-M, NIKKOL Decaglyn 1-SV, NIKKOL Decaglyn 1-50SV, NIKKOL Decaglyn 1-ISV, NIKKOL Decaglyn 1-O, NIKKOL Decaglyn 1-OV, NIKKOL Decaglyn 1-LN, Ryoto-polyglycerester L-7D, L-10D, M-10D, P-8D, SWA-10D, SWA-15D, SWA-20D, S-24D, S-28D, O-15D, O -50D, B-70D, B-100D, ER-60D, LOP-120DP.

The sorbitan fatty acid ester used in the present invention preferably has 8 or more carbon atoms, more preferably 12 or more. Preferred examples of the sorbitan fatty acid ester include sorbitan monocaprylate, sorbitan monolaurate, sorbitan monostearate, sorbitan tristearate, sorbitan tristearate, sorbitan isostearate, sorbitan sesquiisostearate, sorbitan oleate, sorbitan sesquioleate And sorbitan trioleate.
These sorbitan fatty acid esters can be used alone or in combination.
As a commercial item, Nikko Chemicals Co., Ltd. make, NIKKOL SL-10, SP-10V, SS-10V, SS-10MV, SS-15V, SS-30V, SI-10RV, SI-15RV, SO-15 10V, SO-15MV, SO-15V, SO-30V, SO-10R, SO-15R, SO-30R, SO-15EX, manufactured by Daiichi Kogyo Seiyaku Co., Ltd., Sorgen 30V, 40V, 50V, 90, 110, manufactured by Kao Corporation, Rheodor AS-10V, AO-10V, AO-15V, SP-L10, SP-P10, SP-S10V, SP-S30V, SP-O10V, SP-O30V and the like. .

The sucrose fatty acid ester used in the present invention preferably has a fatty acid having 12 or more carbon atoms, more preferably 12-20.
Preferred examples of sucrose fatty acid esters include sucrose dioleate, sucrose distearate, sucrose dipalmitate, sucrose dimyristic ester, sucrose dilaurate, sucrose monooleate, sucrose Examples include sugar monostearate, sucrose monopalmitate, sucrose monomyristic ester, and sucrose monolaurate. Among these, sucrose monooleate, sucrose monostearate, sucrose Monopalmitate, sucrose monomyristate, and sucrose monolaurate are more preferable.
In the present invention, these sucrose fatty acid esters can be used alone or in combination.
Examples of commercially available products include Ryoto Sugar Esters S-070, S-170, S-270, S-370, S-370F, S-570, S-770, and S-970 manufactured by Mitsubishi Chemical Foods Corporation. , S-1170, S-1170F, S-1570, S-1670, P-070, P-170, P-1570, P-1670, M-1695, O-170, O-1570, OWA-1570, L -195, L-595, L-1695, LWA-1570, B-370, B-370F, ER-190, ER-290, POS-135, DK ester SS manufactured by Daiichi Kogyo Seiyaku Co., Ltd., F160, F140, F110, F90, F70, F50, F-A50, F-20W, F-10, F-A10E, Cosmelike B-30, S-10, S-50, S-70, S -110, S-160, S-190, SA-10, SA-50, P-10, P-160, M-160, L-10, L-50, L-160, L-150A, L-160A , R-10, R-20, O-10, O-150 and the like.
Among the above, preferably Leutou Sugar Esters S-1170, S-1170F, S-1570, S-1670, P-1570, P-1670, M-1695, O-1570, L-1695, DK Esters SS, F160, F140, F110, cosmetics S-110, S-160, S-190, P-160, M-160, L-160, L-150A, L-160A, O-150.

The polyoxyethylene sorbitan fatty acid ester used in the present invention preferably has a fatty acid having 8 or more carbon atoms, more preferably 12 or more. Moreover, as length (addition mole number) of the ethylene oxide of polyoxyethylene, 2-100 are preferable and 4-50 are more preferable.
Preferable examples of polyoxyethylene sorbitan fatty acid ester include sorbitan polyoxyethylene monocaprylate, sorbitan polyoxyethylene monolaurate, sorbitan polyoxyethylene monostearate, sorbitan polyoxyethylene dextearate, sorbitan polyoxyethylene tristearate Sorbitan polyoxyethylene isostearate, sorbitan polyoxyethylene sesquiisostearate, sorbitan polyoxyethylene oleate, sorbitan polyoxyethylene sesquioleate, sorbitan polyoxyethylene trioleate, and the like.
These polyoxyethylene sorbitan fatty acid esters can be used alone or in combination.
As a commercial item, Nikko Chemicals Co., Ltd. make, NIKKOL TL-10, NIKKOL TP-10V, NIKKOL TS-10V, NIKKOL TS-10MV, NIKKOL TS-106V, NIKKOL TS-30V, NIKKOL TS-30V, NIKOL TS-30V NIKKOL TO-10V, NIKKOL TO-10MV, NIKKOL TO-106V, NIKKOL TO-30V, manufactured by Kao Corporation, Rheodor TW-L106, TW-L120, TW-P120, TW-S106V, TW-S120V, TW -S320V, TW-O106V, TW-O120V, TW-O320V, TW-IS399C, Rheodor Super SP-L10, TW-L120, manufactured by Daiichi Kogyo Seiyaku Co., Ltd., Sorgen TW-20, TW-60V, TW 80V, and the like.

0.5-30 mass% is preferable with respect to an emulsion composition, and, as for the addition amount of the said surfactant, 1-20 mass% is more preferable, and 2-15 mass% is still more preferable.
By setting the amount of the surfactant to 0.5% by mass or more, the interfacial tension between the oil phase and the water phase can be easily lowered, and by setting the amount to 30% by mass or less, the emulsion composition is not made excessive. This is preferable because it is difficult to cause problems such as excessive foaming.

[Particle size of emulsion composition]
Although the particle diameter of the emulsion composition used for this invention is not specifically limited, It is preferable that it is 200 nm or less, More preferably, it is 150 nm or less, Most preferably, it is 120 nm or less.
By making the particle diameter of an emulsion composition 200 nm or less, it is preferable at the point which the precipitation prevention effect of a coffee bean extract is enhanced. Moreover, it is preferable at the point that the transparency of the drink manufactured using the emulsion is hard to deteriorate, and the intestinal tract absorbability is not easily lowered.

The particle size of the emulsion composition used in the present invention can be measured with a commercially available particle size distribution meter or the like. Emulsion particle size distribution measurement methods include optical microscopy, confocal laser microscopy, electron microscopy, atomic force microscopy, static light scattering, laser diffraction, dynamic light scattering, centrifugal sedimentation, electricity A pulse measurement method, a chromatography method, an ultrasonic attenuation method, and the like are known, and apparatuses corresponding to the respective principles are commercially available.
The dynamic light scattering method is preferred for measuring the emulsion particle size in the present invention because of the particle size range in the present invention and ease of measurement. As a commercially available measuring device using dynamic light scattering, Nanotrac UPA (Nikkiso Co., Ltd.), dynamic light scattering type particle size distribution measuring device LB-550 (Horiba, Ltd.), a concentrated particle size analyzer FPAR-1000 (Otsuka Electronics Co., Ltd.) etc. are mentioned.
The particle diameter in the present invention is a value measured using the dynamic light scattering particle size distribution measuring device LB-550 (Horiba, Ltd.), and specifically, the value measured as follows is adopted. .
The particle diameter is measured by diluting with pure water so that the concentration of the oil component is in the range of 0.1 to 1% by mass and using a quartz cell. The particle diameter can be obtained as a median diameter when the sample refractive index is 1.600, the dispersion medium refractive index is 1.333 (pure water), and the viscosity of the pure water is set as the dispersion medium viscosity.

[Method for producing water-in-oil emulsion composition]
The method for producing the emulsion composition in the present invention is not particularly limited. For example, a) a surfactant is dissolved in an aqueous medium (water, etc.) to obtain an aqueous phase, and b) the oil component (carotenoid, etc.). And an phospholipid (lysolecithin, etc.) are mixed and dissolved to obtain an oil phase, and c) an aqueous phase and an oil phase are mixed with stirring, emulsified and dispersed to obtain an emulsion composition. Is preferred.
The components contained in the oil phase and the aqueous phase in the production method are the same as the constituent components of the emulsion composition of the present invention described above, and preferred examples and preferred amounts are also the same, and preferred combinations are more preferred.

The ratio (mass) of the oil phase and the water phase in the emulsification dispersion is not particularly limited, but the oil phase / water phase ratio (mass%) is preferably 0.1 / 99.9 to 50/50. 0.5 / 99.5-30 / 70 is more preferable, and 1 / 99-20 / 80 is still more preferable.
By setting the oil phase / water phase ratio to 0.1 / 99.9 or more, the active ingredient does not become low, and the practical problem of the emulsion composition tends not to occur, which is preferable. Further, by setting the oil phase / water phase ratio to 50/50 or less, the surfactant concentration does not become thin, and the emulsion stability of the emulsion composition tends not to deteriorate, which is preferable.

The emulsification dispersion may be carried out by one step of emulsification, but it is preferable to carry out two or more steps of emulsification from the viewpoint of obtaining uniform and fine emulsified particles.
Specifically, in addition to a one-step emulsification operation in which emulsification is performed using a normal emulsification apparatus (for example, stirrer, impeller stirring, homomixer, continuous flow shearing apparatus, etc.) using a shearing action, a high-pressure homogenizer, It is particularly preferable to use two or more types of emulsifying devices together by a method such as emulsification through an ultrasonic disperser. By using a high-pressure homogenizer, the emulsion can be arranged into even more uniform droplets of fine particles. Further, it may be performed a plurality of times for the purpose of forming a droplet having a more uniform particle diameter.

<Other ingredients>
The present aqueous composition can contain other components in addition to the above components. In the case of an oil-soluble component, each component can be included as an oil-based component of the emulsion composition, while in the case of a water-soluble component, it may be included in the aqueous phase component of the emulsion composition, You may be contained in the liquid component mix | blended with the said emulsion composition.

[Radical scavenger]
In the present invention, it is preferable to include a radical scavenger in order to prevent oxidation of carotenoids and effectively prevent precipitation of the coffee bean extract.
The radical scavenger is an additive that suppresses the generation of radicals and also captures the generated radicals as quickly as possible and plays a role in breaking the chain reaction (Source: “Oil Chemistry Handbook 4th Edition”, Japan Oil Chemists' Society). Ed. 2001).
As a direct method for confirming the function as the radical scavenger, there is known a method in which the state of trapping radicals by mixing with a reagent is measured by a spectrophotometer or an ESR (electron spin resonance apparatus). In these methods, DPPH (1,1-diphenyl-2-picrylhydrazyl) or galvinoxyl radical is used as a reagent.
In the present invention, the time required to raise the peroxide value (POV value) of fats and oils to 60 meq / kg using the autoxidation reaction of fats and oils under the following experimental conditions is more than twice that of the blank. Certain compounds are defined as “radical scavengers”. The peroxide value (POV value) of fats and oils is measured by a conventional method.
<Conditions>
Oil and fat: Olive oil Sample addition amount: 0.1% by mass based on oil and fat
Test method: The sample was heated at 190 ° C., the POV value was measured by a conventional method over time, and the time for 60 meq / kg was calculated.

The radical scavenger in the present invention is preferably a radical scavenger in which the time required to reach the POV value of 60 meq / kg is 5 times or more that of the blank from the viewpoint of the stability against emulsion oxidation.
Compounds that can be used as radical scavengers of the present invention are described in “Theory and Practice of Antioxidants” (Enomoto, Sanshobo 1984) and “Antioxidants Handbook” (Saruwatari, Nishino, Tabata, Taiseisha 1976). Of the various antioxidants described, any antioxidant can be used as long as it functions as a radical scavenger. Specifically, compounds having phenolic OH, amine compounds such as phenylenediamine, and oils of ascorbic acid and erythorbic acid Examples include solubilized derivatives.

Preferred radical scavengers (antioxidants) below include, for example, (i) a compound group consisting of ascorbic acid or erythorbic acid or a salt thereof, or an ascorbic acid derivative or an erythorbic acid derivative or a salt thereof; There may be mentioned at least two compounds selected from the group consisting of:
The content of the radical scavenger in the emulsion composition is generally 0.001 to 5.0 mass%, preferably 0.01 to 3.0 mass%, more preferably 0.1 to 2.0 mass%. %. Moreover, 10-4000 mg is preferable with respect to 100 ml of aqueous compositions, 50-3000 mg is more preferable, 100-2000 mg is especially preferable. If it is 10 mg or more, the precipitation effect can be expected, and if it is 4000 mg or less, the precipitation effect can be obtained efficiently with respect to the added amount.

(I) Ascorbic acid or erythorbic acid or salts thereof Ascorbic acid or ascorbic acid derivatives or salts thereof include L-ascorbic acid, L-ascorbic acid Na, L-ascorbic acid K, L-ascorbic acid Ca, and L-ascorbic acid phosphorus Acid ester, magnesium salt of L-ascorbic acid phosphate, L-ascorbic acid sulfate, L-ascorbic acid sulfate disodium salt, L-ascorbic acid stearate, L-ascorbic acid 2-glucoside, L-ascorbyl Acid palmitate ester, tetraisopalmitate L-ascorbyl and the like can be mentioned. Among these, L-ascorbic acid, L-ascorbic acid Na, L-ascorbic acid stearate, L-ascorbic acid 2-glucoside, L-ascorbyl palmitate, magnesium salt of L-ascorbic acid phosphate, L-ascorbic acid sulfate disodium salt and L-ascorbyl tetraisopalmitate are particularly preferred.

  As erythorbic acid or erythorbic acid derivatives or salts thereof, erythorbic acid, erythorbic acid Na, erythorbic acid K, erythorbic acid Ca, erythorbic acid phosphate ester, erythorbic acid sulfate ester, erythorbic acid palmitic acid ester, tetraisopalmitic acid erythorbyl, etc. Is mentioned. Of these, erythorbic acid and erythorbic acid Na are particularly preferred.

  As the radical scavenger belonging to the compound group (i) used in the present invention, commercially available ones can be appropriately used. For example, L-ascorbic acid (Takeda Pharmaceutical, Fuso Chemical, BASF Japan, Daiichi Pharmaceutical, etc.), L-ascorbic acid Na (Takeda Pharmaceutical, Fuso Chemical, BASF Japan, Daiichi Pharmaceutical, etc.), Ascorbic acid 2-glucoside (Product name: AA-2G: Hayashibara Biochemical Research Institute), Mg as L-ascorbate phosphate (Product name: Ascorbic acid PM “SDK” (Showa Denko), Product name: NIKKOL VC-PMG (Nikko Chemicals), Product name: Seamate (Takeda) Pharmaceutical industry)), ascorbyl palmitate (DSM Nutrition Japan, Kongo Pharmaceutical, Merck, etc.).

(Ii) Compound group consisting of polyphenols As a compound group consisting of polyphenols, flavonoids (catechin, anthocyanin, flavone, isoflavone, flavan, flavanone, rutin), phenolic acids (chlorogenic acid, ellagic acid, gallic acid, propyl gallate) ), Lignans, curcumins, coumarins and the like. Moreover, since these compounds are contained in a large amount in the following natural product-derived extracts, they can be used in the form of extracts.

  For example, licorice extract, cucumber extract, caquette extract, gentian (gentian) extract, Gentian extract, cholesterol and its derivatives, hawthorn extract, peonies extract, ginkgo biloba extract, scallop (Ogon) extract, carrot Extract, Maika (Maika, Hamanasu) extract, Sunpens (Kawara-Ketsumei) extract, Tormentilla extract, Parsley extract, Button (buttonpi) extract, Mokka (bokeh) extract, Melissa extract, Yashajitsu (Yasha) extract , Yukinoshita extract, Rosemary (mannenrou) extract, lettuce extract, tea extract (Oolong tea, black tea, green tea, etc.), microbial fermentation metabolites, Rakan fruit extract, etc. (in parentheses are plant aliases) , Herbal medicine name etc. were described.) Among these polyphenols, catechin, rosemary extract, glucosyl rutin, ellagic acid, and gallic acid are particularly preferable.

  As the radical scavenger belonging to the compound group (ii) used in the present invention, those commercially available in general can be appropriately used. For example, ellagic acid (Wako Pure Chemicals, etc.), rosemary extract (trade names RM-21A, RM-21E: Mitsubishi Chemical Foods, etc.), catechin (trade names Sancatol W-5, No. 1: Taiyo Kagaku, etc.) , Na gallate (trade name: Sancatol: Taiyo Kagaku, etc.), rutin, glucosylrutin, enzymatically-decomposed rutin (trade names: rutin K-2, P-10: Kiriya Chemical, trade name: αG rutin: Hayashibara Biochemical Laboratory ) And the like.

[Polyhydric alcohol]
The aqueous composition preferably contains a polyhydric alcohol from the viewpoint of particle size, stability, and antiseptic properties, and particularly preferably contained in the emulsion composition.
The polyhydric alcohol has a moisturizing function and a viscosity adjusting function. The polyhydric alcohol also has a function of reducing the interfacial tension between water and an oil and fat component, facilitating the widening of the interface, and facilitating the formation of fine and stable fine particles.
From the above, the emulsion composition contains a polyhydric alcohol from the viewpoint that the emulsion particle diameter can be further refined and can be stably maintained over a long period of time while the particle diameter is in a fine particle diameter state. preferable.
Moreover, the addition of a polyhydric alcohol can lower the water activity of the emulsion composition and suppress the growth of microorganisms.

The polyhydric alcohol that can be used in the present invention is not particularly limited as long as it is a dihydric or higher alcohol.
Examples of the polyhydric alcohol include glycerin, diglycerin, triglycerin, polyglycerin, 3-methyl-1,3-butanediol, 1,3-butylene glycol, isoprene glycol, polyethylene glycol, and 1,2-pentanediol. 1,2-hexanediol, propylene glycol, dipropylene glycol, polypropylene glycol, ethylene glycol, diethylene glycol, pentaerythritol, neopentyl glycol, maltitol, reduced starch syrup, fructose, glucose, sucrose, lactitol, palatinit, erythritol, sorbitol, Mannitol, xylitol, xylose, glucose, lactose, mannose, maltose, galactose, fructose, inositol, pentaerythritol Lumpur, maltotriose, sorbitol, sorbitan, trehalose, starch degradation sugars, starch decomposing sugar reduced alcohol and the like, these may be used alone or in the form of a plurality of kinds of mixtures.

  As the polyhydric alcohol, it is preferable to use a polyhydric alcohol having 3 or more hydroxyl groups in one molecule. Thereby, the interfacial tension between the aqueous solvent and the oil and fat component can be reduced more effectively, and finer and more stable fine particles can be formed. As a result, the intestinal absorbability at the time of oral ingestion can be made higher.

  Among the polyhydric alcohols that satisfy the above-mentioned conditions, particularly when glycerin is used, the particle diameter of the emulsion becomes smaller, and it is preferable because the particle diameter is small and stably maintained over a long period of time. .

The content of the polyhydric alcohol is preferably 10 to 90% by mass, more preferably 25% based on the emulsion composition from the viewpoint of the viscosity of the emulsion composition in addition to the particle diameter, stability and antiseptic properties described above. It is -80 mass%, More preferably, it is 40-70 mass%.
It is preferable that the content of the polyhydric alcohol is 10% by mass or more from the viewpoint that sufficient storage stability is easily obtained depending on the type and content of the fat and oil component. On the other hand, when the content of the polyhydric alcohol is 90% by mass or less, it is preferable in that it is easy to suppress an increase in the viscosity of the emulsion composition.

[Vitamin B]
The aqueous composition can further contain vitamin Bs. In the present invention, as will be described later, even when the pH is high, good storage stability is exhibited. Therefore, vitamin Bs that are likely to be unstable under conditions other than acidity can be preferably contained.
Vitamin B includes vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin B13, biotin, pantothenic acid, nicotinic acid, folic acid, salts and derivatives thereof. Among these, vitamin B1 is preferable because the effect of preventing precipitation can be further enhanced.
Vitamin B1 includes vitamin B1, salts and derivatives thereof, thiamine or a salt thereof, thiamine disulfide, fursultiamine or a salt thereof, dicetiamine, bisbuthiamine, bisbenchamine, benfotiamine, thiamine monophosphate Examples include disulfide, chicotiamine, octotiamine, and prosultiamine.
The amount of vitamin Bs in the present aqueous composition is preferably 1.0 to 100.0 mg, more preferably 1.5 to 80 mg, and particularly preferably 2.0 to 60 mg in 100 ml of the aqueous composition. If the amount is less than 1.0 mg, the effectiveness of vitamin Bs on the human body cannot be fully exerted, and if it exceeds 100 mg, masking of unpleasant odors derived from vitamins becomes difficult.

[Ascorbic acids]
The aqueous composition preferably further contains water-soluble ascorbic acids in order to enhance the precipitation prevention effect of the coffee bean extract.
Ascorbic acids include ascorbic acid, its salts and derivatives. Examples of ascorbate include sodium, potassium, magnesium, and ferrous salts. Ascorbic acid derivatives include stearate, acetate, and palmitate, and other isomers of ascorbic acid. As a certain erythorbic acid and its salt, sodium erythorbate etc. can be mention | raise | lifted, Ascorbic acid and its salt are preferable.
The amount of ascorbic acids in the present aqueous composition is preferably 10 to 4000 mg, more preferably 50 to 3000 mg, and particularly preferably 100 to 2000 mg in 100 ml of the aqueous composition. If it is 10 mg or more, the precipitation prevention effect can be effectively enhanced, and if it is 4000 mg or less, precipitation prevention can be achieved efficiently.

[Other ingredients]
The aqueous composition may contain an organic acid as a pH adjuster or a taste agent. Examples of organic acids include adipic acid, gluconic acid, succinic acid, tartaric acid, acetic acid, citric acid, malic acid, lactic acid, phosphoric acid and the like, and in particular, from the viewpoint of flavor when gluconic acid and citric acid are taken orally To preferred.
The aqueous composition can contain a sweetener as appropriate. The sweetener may be any material that exhibits sweetness. For example, fruit juice, sugars or artificial sweeteners can be used.
The fruit juice may be any fruit juice juice. For example, grapefruit, pineapple, mango, navel orange, Iyokan, Hachiman, summer tangerine, sweet summer tangerine, mandarin orange, lemon, sudachi, kumquat, pomelo, orange, acerola, apple, pear, peach, melon, strawberry, muscat, Examples include grapes such as Concord, Campbell, Delaware, and Kyoho, berries such as blueberries, cranberries, blackcurrants and redcurrants, and fruit juices such as litchi.

Examples of the saccharide include monosaccharides, disaccharides, oligosaccharides, and sugar alcohols. Examples of monosaccharides include glucose, fructose, wood sugar, sorbose, galactose, and isomerized sugar. Examples of disaccharides include sucrose, maltose, lactose, isomerized lactose, and palatinose. Examples of the oligosaccharide include fructooligosaccharide, maltooligosaccharide, isomaltooligosaccharide, galactooligosaccharide, coupling sugar, and palatinose.
Examples of the sugar alcohols include monosaccharide alcohols such as erythritol, sorbitol, xylitol, and mannitol, disaccharide alcohols such as maltitol, isomaltitol, and lactitol, maltotriitol, isomaltitol, and panitol. Examples thereof include trisaccharide alcohols, oligosaccharide alcohols and higher tetrasaccharide alcohols, powdered reduced maltose starch syrup and the like.
Examples of the artificial sweetener include stevia, aspartame, saccharin, glutyllithin, thaumatin, and sucralose.

A perfume can be appropriately contained in the aqueous composition. Examples of the fragrances include natural fragrances and synthetic fragrances. As said natural fragrance | flavor, the fragrance | flavor component containing material etc. which were prepared according to the conventional method, for example using grassroots, a bark, a flower, a fruit, fruit skin, or other animals and plants, etc. are mentioned. The natural fragrances include essential oils and the like obtained by treating natural materials by a steam distillation method, a pressing method, an extraction method, or the like.
Examples of the synthetic fragrance include coffee-derived fragrance, black tea-derived fragrance, green tea-derived fragrance, oolong tea-derived fragrance, cocoa-derived fragrance, herb-derived fragrance, spice-derived fragrance, and fruit-derived fragrance.

[PH of aqueous composition]
The pH of the aqueous composition is not particularly limited, but in order to further exhibit the precipitation-preventing effect of the present invention, it is preferably acidic, particularly pH 1.0 to 6.0 at 20 ° C., more preferably It is 2.0-5.0, More preferably, it is 2.5-4. When the pH is higher than 6.0, the precipitation of coffee beans is less likely to be a problem. When the pH is lower than 1.0, the irritation is strong and may not be suitable for citation.
In addition, if the aqueous composition of this invention is a liquid composition containing a coffee bean extract, any thing may be sufficient and a drink, cosmetics, an injection, a liquid agent etc. can be mentioned. In particular, a beverage is preferable because the coffee bean extract can be easily ingested for uses such as a functional drink.

<Contained beverage>
The container-packed beverage of the present invention can be easily obtained by filling the above-described coffee bean extract-containing aqueous composition containing a coffee bean extract and oil-in-water emulsion particles containing carotenoids into a predetermined container. Obtainable.
This container-packed beverage is a form in which a coffee bean extract-containing aqueous composition is applied as a beverage, and it contains not only a small amount of precipitate but also high transparency, and also contains functional components such as carotenoids. Expected functions can also be expected.

For each component in the container-packed beverage, the matters described for the coffee bean extract-containing aqueous composition, including the type and blending amount, can be applied as they are.
In particular, these components are preferably included because the taste and the like as a beverage can be appropriately adjusted by blending a flavoring agent, a sweetening agent, a flavoring agent, and the like.

  As a container used for this container-packed drink, what is normally used as a container for drinks should just be mentioned, For example, a PET bottle, a paper pack, a glass container, an aluminum can, a steel can etc. can be mentioned.

<Precipitation prevention method>
The method for preventing precipitation of a coffee bean extract according to the present invention includes blending an oil-in-water emulsion composition containing carotenoids with an aqueous composition containing a coffee bean extract.
As described above, the presence of oil-in-water emulsion particles containing carotenoids in the aqueous composition can effectively prevent precipitation of the coffee bean extract. The above items can be applied as they are to this precipitation prevention method.

  In this precipitation prevention method, an oil-in-water emulsion composition containing carotenoids in an amount that can prevent precipitation of the coffee bean extract may be blended, and 0.02% by mass to 20% by mass with respect to the entire aqueous composition. % Is preferable, more preferably 0.1% by mass to 10% by mass, and still more preferably 0.2% to 4.0% by mass. If it is 0.02% by mass or more, the dispersion of the coffee bean extract can be maintained while ensuring the function of the functional component, and if it is 20% by mass or less, the emulsion particles can be efficiently blended, which is preferable respectively. .

The effect of preventing precipitation in the present invention is, for example, that an aqueous composition containing a coffee bean extract and oil-in-water emulsion particles containing carotenoids is produced as described above, or a coffee bean extract-containing aqueous composition. It can be evaluated by adding an oil-in-water emulsion containing carotenoids as described above and allowing to stand at a predetermined temperature.
The low temperature at which the precipitation preventing effect of the present invention is obtained may be any temperature that does not freeze, and can generally be 10 ° C. or lower, preferably 5 ° C. or lower. Moreover, instability means temperature change refers to a condition that changes repeatedly between the low temperature and a higher temperature. The high temperature in this temperature change means a high temperature that does not denature the contained components, generally 60 ° C. or less, preferably 50 ° C. or less.
In any of these conditions, the aqueous composition of the present invention can prevent precipitation from occurring for 3 months or more, preferably 4 months or more, more preferably 5 months or more.

  The present invention will be described more specifically with reference to the following examples. In the following description, “parts” and “%” are based on mass unless otherwise specified.

<Preparation of carotenoid-containing emulsion composition>
(1) EM-01
The following components were dissolved for 1 hour while heating at 70 ° C. to obtain an aqueous phase composition.
・ Sucrose stearate (HLB = 16) 60.0 g
・ Decaglyceryl monooleate (HLB = 12) 60.0 g
・ Glycerin 630.0g
・ Pure water 160.0g

Moreover, the following component was melt | dissolved for 1 hour, heating at 70 degreeC, and the oil phase composition was obtained.
・ Hematococcus alga extract (astaxanthin content 20% by mass) 25.0 g
・ Mixed Tocopherol 1.0g
・ Coconut oil 59.0g
・ Lysolecithin 5.0g

The aqueous phase composition is stirred at a homogenizer (model name HP93, manufactured by SMT Co., Ltd.) while maintaining the temperature at 70 ° C. (10000 rpm), and the oil phase composition is added to the aqueous phase composition to obtain an emulsion composition. Got.
Subsequently, the pre-emulsion composition obtained by cooling and room temperature was subjected to high-pressure emulsification at 200 MPa using an optimizer HJP-25005 (manufactured by Sugino Machine Co., Ltd.).
Then, it filtered with the micro filter with an average hole diameter of 1 micrometer, and prepared the astaxanthin containing emulsion composition EM-01.

  1.0 g of the astaxanthin-containing emulsion composition (EM-01) obtained above was added to 99.0 g of pure water and stirred for 10 minutes using a stirrer. It was 96 nm when the particle diameter of the obtained emulsion water dilution liquid was measured at 25 degreeC using the dynamic light-scattering particle size dispersion measuring device LB-550 (made by Horiba, Ltd.).

(2) EM-02
The following components were dissolved for 1 hour while heating at 70 ° C. to obtain an aqueous phase composition.
・ Sucrose stearate (HLB = 16) 50.0 g
・ Decaglyceryl monooleate (HLB = 12) 50.0 g
・ Glycerin 630.0g
・ Pure water 180.0g

Moreover, the following component was melt | dissolved for 1 hour, heating at 70 degreeC, and the oil phase composition was obtained.
・ Hematococcus alga extract (astaxanthin content 20% by mass) 25.0 g
・ Mixed Tocopherol 1.0g
・ Coconut oil 59.0g
・ Lysolecithin (derived from soybean) 5.0 g

  Emulsification was performed in the same manner as described above to prepare an astaxanthin-containing emulsion composition EM-02.

  In the same manner as described above, the particle diameter of the astaxanthin-containing emulsion composition (EM-02) obtained above was measured and found to be 148 nm.

(3) EM-03
The following components were dissolved for 1 hour while heating at 70 ° C. to obtain an aqueous phase composition.
・ Sucrose stearate (HLB = 16) 30.0 g
・ Decaglyceryl monooleate (HLB = 12) 30.0 g
・ Glycerin 630.0g
・ Pure water 220.0g

Moreover, the following component was melt | dissolved for 1 hour, heating at 70 degreeC, and the oil phase composition was obtained.
・ Hematococcus alga extract (astaxanthin content 20% by mass) 25.0 g
・ Mixed Tocopherol 1.0g
・ Coconut oil 59.0g
・ Lysolecithin (derived from soybean) 5.0 g

  Emulsification was performed in the same manner as described above to prepare an astaxanthin-containing emulsion composition EM-03.

  In the same manner as described above, the particle diameter of the astaxanthin-containing emulsion composition (EM-03) obtained above was measured and found to be 248 nm.

(4) EM-04
The following components were dissolved for 1 hour while heating at 70 ° C. to obtain an aqueous phase composition.
・ Sucrose stearate (HLB = 16) 60.0g
・ Decaglyceryl monooleate (HLB = 12) 60.0 g
・ Glycerin 630.0g
・ Pure water 160.0g

Moreover, the following component was melt | dissolved for 1 hour, heating at 70 degreeC, and the oil phase composition was obtained.
Β-carotene 30% suspension (β-carotene content 30% by mass) 16.7 g
・ Mixed Tocopherol 1.0g
・ Coconut oil 67.3g
・ Lysolecithin 5.0g

  Emulsification was performed in the same manner as described above to prepare β-carotene-containing emulsion composition EM-04.

  In the same manner as described above, the particle diameter of the β-carotene-containing emulsion composition (EM-04) obtained above was measured and found to be 111 nm.

(5) EM-05
The following components were dissolved for 1 hour while heating at 70 ° C. to obtain an aqueous phase composition.
・ Sucrose stearate (HLB = 16) 60.0 g
・ Decaglyceryl monooleate (HLB = 12) 60.0 g
・ Glycerin 630.0g
・ Pure water 160.0g

Moreover, the following component was melt | dissolved for 1 hour, heating at 70 degreeC, and the oil phase composition was obtained.
・ Tomato extract (lycopene content 18% by mass) 27.8 g
・ Mixed Tocopherol 1.0g
・ Coconut oil 56.2g
・ Lysolecithin 5.0g

  Emulsification was performed in the same manner as described above to prepare lycopene-containing emulsion composition EM-05.

  As described above, the particle size of the lycopene-containing emulsion composition (EM-05) obtained above was measured and found to be 106 nm.

<Manufacture of coffee bean extract-containing beverage>
[Example 1]
・ EM-01 1.60% by weight
・ Raw coffee bean extract (powder) 0.40% by weight
・ Vitamin B1 (thiamine nitrate) 0.01% by weight
・ Erythritol 8.00% by weight
-Fragrance (orange flavor) 0.30% by weight
After adding and dissolving the above in pure water, citric acid was added until the pH of the solution reached 3.5 (20 ° C.) to prepare 5000 ml of a liquid composition. This was sterilized at 80 ° C. for 30 minutes, and 50 ml was dispensed and sealed in a glass bottle container to obtain a beverage.

[Example 2]
EM-02 1.60% by weight
・ Raw coffee bean extract (powder) 0.40% by weight
・ Vitamin B1 (thiamine nitrate) 0.01% by weight
・ Erythritol 8.00% by weight
-Fragrance (orange flavor) 0.30% by weight
After adding and dissolving the above in pure water, citric acid was added until the pH of the solution reached 3.5 (20 ° C.) to prepare 5000 ml of a liquid composition. This was sterilized at 80 ° C. for 30 minutes, and 50 ml was dispensed and sealed in a glass bottle container to obtain a beverage.

[Example 3]
・ EM-03 1.60% by weight
・ Raw coffee bean extract (powder) 0.40% by weight
・ Vitamin B1 (thiamine nitrate) 0.01% by weight
・ Erythritol 8.00% by weight
-Fragrance (orange flavor) 0.30% by weight
After adding and dissolving the above in pure water, citric acid was added until the pH of the solution reached 3.5 (20 ° C.) to prepare 5000 ml of a liquid composition. This was sterilized at 80 ° C. for 30 minutes, and 50 ml was dispensed and sealed in a glass bottle container to obtain a beverage.

[Example 4]
EM-04 1.60% by weight
・ Raw coffee bean extract (powder) 0.40% by weight
・ Vitamin B1 (thiamine nitrate) 0.01% by weight
・ Erythritol 8.00% by weight
-Fragrance (orange flavor) 0.30% by weight
After adding and dissolving the above in pure water, citric acid was added until the pH of the solution reached 3.5 (20 ° C.) to prepare 5000 ml of a liquid composition. This was sterilized at 80 ° C. for 30 minutes, and 50 ml was dispensed and sealed in a glass bottle container to obtain a beverage.

[Example 5]
・ EM-05 1.60% by weight
・ Raw coffee bean extract (powder) 0.40% by weight
・ Vitamin B1 (thiamine nitrate) 0.01% by weight
・ Erythritol 8.00% by weight
-Fragrance (orange flavor) 0.30% by weight
After adding and dissolving the above in pure water, citric acid was added until the pH of the solution reached 3.5 (20 ° C.) to prepare 5000 ml of a liquid composition. This was sterilized at 80 ° C. for 30 minutes, and 50 ml was dispensed and sealed in a glass bottle container to obtain a beverage.

[Example 6]
・ EM-01 1.60% by weight
・ Raw coffee bean extract (powder) 0.40% by weight
・ Erythritol 8.00% by weight
-Fragrance (orange flavor) 0.30% by weight
The above was added to and dissolved in pure water to prepare 5000 ml of a liquid composition. This was sterilized at 80 ° C. for 30 minutes, and 50 ml was dispensed and sealed in a glass bottle container to obtain a beverage. The pH (20 ° C.) was 6.0.

[Example 7]
・ EM-01 1.60% by weight
・ Raw coffee bean extract (powder) 0.50% by weight
・ Vitamin B1 (thiamine nitrate) 0.01% by weight
・ Ascorbic acid 0.50% by weight
・ Sodium ascorbate 0.50% by weight
・ Erythritol 8.00% by weight
-Fragrance (orange flavor) 0.30% by weight
After adding and dissolving the above in pure water, citric acid was added until the pH of the solution reached 3.5 (20 ° C.) to prepare 5000 ml of a liquid composition. This was sterilized at 80 ° C. for 30 minutes, and 50 ml was dispensed and sealed in a glass bottle container to obtain a beverage.

[Comparative Example 1]
・ Raw coffee bean extract (powder) 0.40% by weight
・ Vitamin B1 (thiamine nitrate) 0.01% by weight
・ Erythritol 8.00% by weight
-Fragrance (orange flavor) 0.30% by weight
After adding and dissolving the above in pure water, citric acid was added until the pH of the solution reached 3.5 (20 ° C.) to prepare 5000 ml of a liquid composition. This was sterilized at 80 ° C. for 30 minutes, and 50 ml was dispensed and sealed in a glass bottle container to obtain a beverage.

[Comparative Example 2]
・ Raw coffee bean extract (powder) 0.40% by weight
・ Erythritol 8.00% by weight
-Fragrance (orange flavor) 0.30% by weight
The above was added to and dissolved in pure water to prepare 5000 ml of a liquid composition. This was sterilized at 80 ° C. for 30 minutes, and 50 ml was dispensed and sealed in a glass bottle container to obtain a beverage. The pH (20 ° C.) was 6.0.

[Comparative Example 3]
・ Raw coffee bean extract (powder) 0.50% by weight
・ Vitamin B1 (thiamine nitrate) 0.01% by weight
・ Ascorbic acid 0.50% by weight
・ Sodium ascorbate 0.50% by weight
・ Erythritol 8.00% by weight
-Fragrance (orange flavor) 0.30% by weight
After adding and dissolving the above in pure water, citric acid was added until the pH of the solution reached 3.5 (20 ° C.) to prepare 5000 ml of a liquid composition. This was sterilized at 80 ° C. for 30 minutes, and 50 ml was dispensed and sealed in a glass bottle container to obtain a beverage.

[Evaluation test]
(1) Test example 1
Each of 50 beverages prepared in Examples 1 to 7 and Comparative Examples 1 to 3 was allowed to stand at 4 ° C. for 6 months. Meanwhile, the state in the beverage was observed, and the period until precipitation occurred was examined.
(2) Test example 2
Each of the 50 beverages prepared in Examples 1 to 7 and Comparative Examples 1 to 3 was allowed to stand for 6 months under the conditions of repeating 4 ° C., 12 hours and 40 ° C., 12 hours. Meanwhile, the state in the beverage was observed, and the period until precipitation occurred was examined.

Table 1 shows the period until the occurrence of precipitation of each beverage in Test Examples 1 and 2.
As shown in Table 1, all the beverages of Examples 1 to 7 were able to prevent the occurrence of precipitation over a longer period than the beverages of Comparative Examples 1 to 3.
Moreover, the smaller the particle size of the emulsion particles, the more remarkable the precipitation preventing effect. Further, the precipitation preventing effect could be further improved by adding a radical scavenger.

[Example 8]
・ EM-01 1.60% by weight
・ Raw coffee bean extract (powder) 0.50% by weight
・ Coenzyme Q10 aqueous solution (containing 10% by weight of Coenzyme Q10) 0.60% by weight
・ L-carnitine 0.50% by weight
・ Caffeine 0.10% by weight
・ Vitamin B1 (thiamine nitrate) 0.01% by weight
・ Vitamin B2 (sodium riboflavin phosphate) 0.01% by weight
・ Vitamin B6 (pyridoxine hydrochloride) 0.01% by weight
・ Nicotinamide 0.01% by weight
・ Ascorbic acid 0.50% by weight
・ Sodium ascorbate 0.50% by weight
・ Erythritol 8.00% by weight
・ Sucralose 0.01% by weight
・ Citric acid 0.60% by weight
-Fragrance (orange flavor) 0.30% by weight

After adding and dissolving the above in pure water, an aqueous gluconic acid solution (containing 50% by weight of gluconic acid) was added until the pH of the solution reached 3.5 (20 ° C.) to prepare 5000 ml of an aqueous composition. This was sterilized at 80 ° C. for 30 minutes, and 50 ml was dispensed and sealed in a glass bottle container to obtain a beverage.
When the beverage obtained in Example 8 was tested by the methods described in Test Examples 1 and 2, no precipitation was observed after 6 months in both methods.

  As described above, according to the present invention, it is possible to effectively prevent the occurrence of precipitates in the aqueous composition containing the coffee bean extract even at low temperatures or under unstable temperature conditions. It is possible to provide a coffee bean extract-containing aqueous composition and a container-packed beverage in which occurrence of food is suppressed.

Claims (23)

  A coffee bean extract-containing aqueous composition comprising a coffee bean extract and oil-in-water emulsion particles containing carotenoids.   The composition according to claim 1, wherein the carotenoids are astaxanthins.   The composition according to claim 1 or 2, wherein the coffee bean extract is a green coffee bean extract.   The composition according to any one of claims 1 to 3, wherein the pH is 6 or less.   The composition according to any one of claims 1 to 4, further comprising vitamin Bs.   The composition according to claim 5, wherein the vitamin B is vitamin B1.   The composition according to any one of claims 1 to 6, wherein a particle diameter of the emulsion particles is 200 nm or less.   A packaged beverage comprising a coffee bean extract and oil-in-water emulsion particles containing carotenoids.   The packaged beverage according to claim 8, wherein the carotenoids are astaxanthins.   The packaged beverage according to claim 8 or 9, wherein the coffee bean extract is a fresh coffee bean extract.   pH is 6 or less, The container-packed drink of any one of Claims 8-10.   The container-packed drink of any one of Claims 8-11 which further contains vitamin B.   The packaged beverage according to claim 12, wherein the vitamin B is vitamin B1.   The container-packed drink according to any one of claims 8 to 13, wherein a particle diameter of the emulsion particles is 200 nm or less.   The container-packed drink of any one of Claims 8-14 which further contains ascorbic acids.   A method for producing a coffee bean extract-containing aqueous composition comprising blending a liquid component containing a coffee bean extract and an oil-in-water emulsion composition containing carotenoids.   The method according to claim 16, wherein the carotenoids are astaxanthins.   The method according to claim 16 or 17, wherein the particle diameter of the emulsion is 200 nm or less.   The method according to any one of claims 16 to 18, further comprising containing ascorbic acids.   A method for preventing precipitation of a coffee bean extract, comprising blending an aqueous composition containing a coffee bean extract with an oil-in-water emulsion composition containing a carotenoid.   The method according to claim 20, wherein the carotenoids are astaxanthins.   The method according to claim 20 or 21, wherein the particle diameter of the emulsion is 200 nm or less.   The method according to any one of claims 20 to 22, further comprising ascorbic acids.