JP4887573B2 - Allergen inactivating agent - Google Patents

Description

[0001]
BACKGROUND OF THE INVENTION
The present invention relates to an allergen inactivating agent.
[0002]
[Background Art and Problems to be Solved by the Invention]
Many people continue to suffer from various allergies such as hay fever and asthma. However, the fundamental treatment method for allergies has not yet been established, and in order to control allergies, allergen-causing substances called allergens are removed from the body by cleaning, etc., and practically other than avoiding contact with allergens as much as possible. There was no effective means.
In recent years, a method for inactivating allergens using compounds such as gallic acid and hydroxyapatite has been proposed (JP-A-6-279273). Such a method has attracted attention as an effective means for allergy control.
[0003]
[Means for Solving the Problems]
As a result of intensive studies on inactivation of allergens under these circumstances, the present inventors have found that a pomegranate (Punica granatum) leaf extract, which is a member of the family Pomegranate, has the ability to inactivate allergens. The present invention has been reached.
[0004]
That is, the present invention contains, as an active ingredient, a pomegranate leaf extract (hereinafter referred to as the present active ingredient) using a solvent (hereinafter referred to as an extraction solvent) comprising at least one selected from water and a hydrophilic solvent. An allergen inactivating agent (hereinafter referred to as the allergen inactivating agent of the present invention) and an allergen inactivating method characterized in that the allergen inactivating agent of the present invention is treated in the area where the allergen is present. To do.
[0005]
DETAILED DESCRIPTION OF THE INVENTION
Pomegranate (Punica granatum) is a member of the family Pomegranate, and its fruits are used for food throughout the world.
In the present invention, the pomegranate is a leaf portion. The pomegranate leaf may be in a raw state or a dried state, and may be in an intact form or in a crushed form.
[0006]
The active ingredient used in the present invention is a brown powder and can be obtained, for example, by the following method.
Pomegranate leaves are immersed in the extraction solvent for a while. Thereafter, the pomegranate leaf is taken out by a method such as filtration, and the remaining extraction solvent is removed by a method such as distillation or lyophilization.
[0007]
Immersion is a treatment of bringing a plant such as leaves into contact with a solvent in order to extract an active ingredient in the solvent. In the present invention, specifically, there is a method of immersing pomegranate leaves in an extraction solvent. Can be mentioned. Immersion can be performed, for example, under standing conditions or under stirring conditions, and can be performed under room temperature conditions or heating conditions.
[0008]
When the extraction solvent is a hydrophilic solvent or a mixed solvent composed of water and a hydrophilic solvent, the amount of the extraction solvent for pomegranate leaves is usually 3 to 20 L per 1 kg of pomegranate leaves.
The immersion temperature is from room temperature to 80 ° C, usually from 10 to 50 ° C, preferably from 15 to 30 ° C.
The immersion time is about 1 day to 2 months, usually 1 to 5 days.
[0009]
When the extraction solvent is water, the immersion temperature is preferably 50 ° C. or higher. Immersion is usually performed under heating conditions, and in practice, it is performed under temperature conditions at or near 100 ° C. In that case, you may go while adding water on the way.
The immersion time is about 1 to 24 hours, usually 2 to 12 hours.
[0010]
Prior to immersion in the pomegranate leaf extraction solvent, impurities such as lipids and fatty acids can be removed by pretreatment with a hydrophobic solvent.
The pretreatment is performed by immersing pomegranate leaves in a hydrophobic solvent. The amount of the hydrophobic solvent used for the pretreatment is usually 3 to 20 L per 1 kg of pomegranate, and the pretreatment temperature is 15 to 30. ° C.
The pretreatment time is about 1 to 30 days, usually 24 to 72 hours.
[0011]
In the present invention, the hydrophilic solvent means an organic solvent capable of forming a uniform solvent with water at 15 to 30 ° C. regardless of the mixing ratio.
[0012]
Examples of the hydrophilic solvent include alcohols having 1 to 3 carbon atoms such as methanol, ethanol and isopropyl alcohol, glycols such as ethylene glycol and propylene glycol, and ketones such as acetone. It may be a mixture of seeds or more.
[0013]
The hydrophobic solvent can be defined as an organic solvent that separates into two layers when mixed with the same volume of water at 15 to 30 ° C., and specifically includes ethers such as diethyl ether and t-butyl methyl ether. , Esters such as ethyl acetate and butyl acetate, halogenated hydrocarbon solvents such as dichloroethane, trichloroethane and chloroform, aromatic hydrocarbons such as benzene, toluene and xylene, aliphatic hydrocarbons such as pentane and hexane, etc. Can be mentioned. Moreover, these may be a mixture of two or more.
[0014]
As the extraction solvent, hydrophilic solvents are preferable, and alcohols having 1 to 3 carbon atoms are particularly preferable.
[0015]
The allergen inactivating agent of the present invention contains the active ingredient in its form as it is, an aqueous solution (aqueous solution), an oil, an emulsion, a powder, a suspension, a wettable powder, a spray, an aerosol, and a coating agent. It can be in various forms such as the form of the preparation (hereinafter referred to as the present preparation).
[0016]
The preparation is usually prepared by a generally known method in which the active ingredient is mixed with a carrier such as a liquid carrier or a solid carrier, and an auxiliary agent for preparation is added if necessary.
[0017]
Examples of the liquid carrier include water; monovalent alcohols having 1 to 6 carbon atoms such as methanol, ethanol, propanol, isopropyl alcohol, and amyl alcohol; carbon numbers such as glycerin, butyl diglycol, ethyl diglycol, ethylene glycol, and diethylene glycol. Is a polyhydric alcohol having 2-6, amides such as N, N-dimethylformamide, N, N-dimethylacetamide, ethers such as diethyl ether, ethylene glycol dimethyl ether, tetrahydrofuran, dioxane, acetone, methyl ethyl ketone, methyl isobutyl ketone, Ketones such as cyclohexanone, hexane, kerosene, paraffin, lanolin derivatives, aliphatic hydrocarbons such as petroleum benzine, esters such as ethyl acetate, nitriles such as acetonitrile, Synthesis fats Con oil, soybean oil, vegetable oils such as cottonseed oil, dimethyl sulfoxide, polyethylene glycol, and the like.
[0018]
Examples of the solid carrier include mineral powders such as silicic acid, kaolin, activated carbon, bentonite, diatomaceous earth, and talc, vegetable powders such as wheat flour and starch, and synthetic polymer powders such as polyvinyl chloride.
[0019]
Examples of the formulation adjuvant include emulsifiers, dispersants, spreading / wetting agents, suspending agents, preservatives, propellants, thickeners, antioxidants, ultraviolet absorbers, and film forming agents. .
Specifically, for example, soaps as an emulsifier, polyoxyethylene fatty acid alcohol ethers such as polyoxyethylene oleyl ether, polyoxyethylene alkyl allyl ethers such as polyoxyethylene nonylphenyl ether, polyoxyethylene fatty acid esters, fatty acid glycerides, sorbitan Fatty acid esters, sulfates of higher alcohols, alkylaryl sulfonates such as sodium dodecylbenzenesulfonate, etc., as a dispersant, decaglyceryl monomyristate, decaglyceryl monostearate, decaglyceryl monooleate, decaglyceryl monolinoleate, Polyoxyethylene sorbitan monostearate, polyoxyethylene sorbitan monooleate, polyoxyethylene cetyl ether, polyoxyethylene oleit Ether, polyoxyethylene stearyl amine and the like, and the spreading-wetting agents, glycerine may also be used as liquid carriers, polyethylene glycol, and the like. Casein, gelatin, alginic acid, carboxymethylcellulose, gum arabic, hydroxypropylcellulose, bentonite, etc. as suspending agents, methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, butyl paraoxybenzoate, etc. as preservatives, propellant Liquefied petroleum gas, dimethyl ether, chlorofluorocarbons, nitrogen gas, carbon dioxide gas, liquefied carbon dioxide gas, Freon gas (Freon 11, Freon 12, etc.), pentane, etc., thickeners as hydroxyethylcellulose, methylcellulose, ethylcellulose, carboxymethylcellulose, gum arabic , Polyvinyl alcohol, polyvinyl methacrylate, carrageenan, pectin, alginic acid, etc., tocopherol, di-t-butylhydroxyl as an antioxidant Cytoluene (BHT), di-t-butylhydroxyanisole (BHA), ascorbic acid, sesamol, isopropyl gallate, etc., p-aminobenzoic acid derivatives, salicylic acid derivatives, anthranilic acid derivatives, benzotriazole derivatives, coumarins, as UV absorbers Examples of coating film forming agents such as benzophenone and guaiazulene include polyvinyl alcohol.
In this preparation, the active ingredient is usually contained in an amount of 0.1 to 50% by weight, preferably 1 to 30% by weight.
[0020]
Sprays include, for example, emulsions containing this active ingredient, suspensions, wettable powders, etc., if necessary, diluted with water together with dispersants and surfactants, aqueous solutions containing this active ingredient, or this effective It is obtained by filling the oil agent containing a component into a container having a spray mechanism.
Aerosols, for example, contain emulsions, suspensions, etc. containing this active ingredient, if necessary, diluted with water together with dispersants, surfactants, etc., aqueous solutions containing this active ingredient, or this active ingredient It is obtained by filling the oil agent with a propellant or the like in a pressure-resistant container provided with an injection port.
The coating agent can be obtained, for example, by adding a film forming agent or the like to the aqueous solution containing the active ingredient as necessary.
[0021]
Examples of the organic solvent contained in the oil include organic solvents that can dissolve the active ingredient, and specifically include ethers such as diethyl ether and aliphatic hydrocarbons such as pentane and hexane.
[0022]
The allergen can be inactivated by treating the allergen inactivating agent of the present invention in the region where the allergen is present.
[0023]
In the present invention, the inactivation of allergen means that the allergen-induced action is lost.
[0024]
The allergen inactivating agent of the present invention is treated by a method such as spraying, spraying or coating in the allergen existing area.
[0025]
Examples of allergens here include mites (adult mites, mite carcasses, tick feces, etc.), animal allergens such as animal hair, plant allergens such as pollen, and the like.
Examples of mites that can be allergens include indoor dust mites, and specifically, Dermatophagoides farmae, Dermatophagoides pteronyssinus, and the like.
[0026]
Specific examples of the allergen-existing region where the allergen inactivating agent of the present invention is treated include floors, wall surfaces, and household items to which allergens adhere. Examples of household items include tatami mats, sofas, and cushions. And carpets, mats, carpets, blankets, pillows, futons and mattresses.
[0027]
The allergen inactivating agent of the present invention includes, for example, a method of spraying a powder on the surface of floors, daily necessities, etc., a method of spraying aerosols, sprays, etc. on the surfaces of floors, wall surfaces, daily necessities, cushions, pillows, etc. The allergen-existing region is treated by spraying aerosol agent, spraying agent, etc. on the batting of daily necessities such as futons, mattresses, etc., applying the coating agent on floors, wall surfaces, etc.
[0028]
The treatment amount of the allergen inactivating agent of the present invention is 1 m when spraying dust on the surface of floors, daily necessities, etc., or when spraying aerosols, sprays, etc. on the surfaces of floors, wall surfaces, daily necessities, etc. It is usually 0.1 to 50 g, preferably 1 to 10 g in terms of the amount of active ingredients per ² . Carpets, carpets and the like are usually treated with 5 to 30 g in terms of the amount of the active ingredient per 1 m ² . In addition, when spraying aerosols, sprays, etc. on the fillings of daily necessities such as cushions, pillows, futons, mattresses, etc., the treatment amount is usually 0.1-50 g in terms of the amount of this active ingredient per 1 m ^3. , Preferably 1 to 5 g. When the coating agent is applied to a floor, a wall surface or the like, the treatment amount is usually 0.1 to 50 g, preferably 1 to 10 g in terms of the amount of the active ingredient per 1 m ² .
The time required to inactivate the allergen with the allergen inactivating agent of the present invention is usually 1 to 3 hours after the treatment with the allergen inactivating agent of the present invention. In addition, the allergen inactivating agent of the present invention is removed from the application site by a vacuum cleaner or the like as necessary after allergen inactivation.
[0029]
The allergen inactivating agent of the present invention contains an acaricide and its synergist, bactericides, fungicides and the like as necessary. Specifically, for example, as an acaricide, pyrethroid compounds such as phenothrin, permethrin, enpentrin, praretrin, phenyl salicylate, benzyl benzoate, 2-methoxycarbonyl-4-chlorotrifluoromethanesulfonanilide, etc. Piperonyl butoxide, N- (2-ethylhexyl) -1-isopropyl-4-methylbicyclo [2,2,2] -oct-5-ene-2,3-dicarboximide, N- (2-ethynyl) -bicyclo [2,2,1] -hepta-5-ene-2,3-dicarboximide and the like, tricyclosan (2,4,4′-trichloro-2) as fungicide and fungicide '-Hydroxydiphenyl ether), chlorohexidine hydrochloride, p-chloro-m-xylenol, 3-methyl-4-isopropyl Pyrphenol, carvacrol, thymol, hinokitiol, catechin, 2- (4-thiazolyl) -benzimidazole, methyl-2-benzimidazole carbamate, benzalkonium chloride, N- (trichloromethylthio) -4-cyclohexe-1, Examples include 2-dicarbimide, N- (fluorodichloromethylthio) phthalimide, 2,4,5,6-tetrachloroisophthalonitrile, 2,3,5,6-tetrachloro-4- (methylsulfonyl) pyridine, and the like.
[0030]
The allergen inactivating agent of the present invention contains a vegetable essential oil having a fragrance as necessary. Examples of aromatic plant oils include carrot oil, apple oil, strawberry oil, lemon oil, citronella oil, orange oil, rosemary oil, fennel oil, anise oil, peppermint oil, rose oil, eucalyptus oil, palmarosa oil, clove Oil, pine needle oil, abies oil, ylang ylang oil, cinnamon oil, caraway oil, cypress oil, cypress oil, ginger oil, thyme oil, oregano oil, perilla oil, cumin oil, hyssop oil, manuka oil, etc. It is done.
The amount is within a range that does not impair the effect of the allergen inactivating agent of the present invention, and may vary depending on the strength of the fragrance of the essential oil, but is usually 0.01 to 10% by weight, preferably 0. 1 to 5% by weight.
[0031]
In addition, allergens can be prevented by treating the allergen inactivating agent of the present invention in advance on the floor, wall surface, daily necessities, etc. before the allergen adhesion or before the occurrence of mites.
[0032]
【Example】
Hereinafter, although the formulation example and test example of this invention allergen inactivating agent are given and this invention is demonstrated further in detail, this invention is not limited to these examples.
First, formulation examples of the allergen inactivating agent of the present invention are shown. Unless otherwise indicated, parts represent parts by weight.
[0033]
Formulation Example 1
1000 g of fully dried pomegranate leaves were immersed in 10 L of chloroform for 1 week at room temperature. Then, the pomegranate leaf was taken out by filtration, and the pomegranate leaf was then immersed in 10 L of methanol for 1 week. The pomegranate leaf was removed by filtration, and methanol was distilled off from the remaining solution by an evaporator to obtain 45 g of a brown and powdery pomegranate leaf methanol extract.
[0034]
Formulation Example 2
1000 g of fully dried pomegranate leaves were immersed in 10 L of chloroform for 1 week at room temperature. Thereafter, the pomegranate leaves were removed by filtration, and the pomegranate leaves were then added to 5 L of water and boiled for 2 hours with occasional addition of water. After leaving for a while to return to room temperature, the pomegranate leaves were removed by filtration. The remaining solution was freeze-dried to obtain 100 g of a brown, powdery pomegranate water extract.
[0035]
Formulation Example 3
10 parts of a pomegranate methanol extract, 40 parts of ethanol, 20 parts of benzyl alcohol, and 30 parts of water are thoroughly stirred and mixed to obtain an aqueous solution.
[0036]
Formulation Example 4
5 parts of a pomegranate methanol extract, 10 parts of ethanol and 85 parts of water are thoroughly mixed with stirring to obtain an aqueous solution.
[0037]
Formulation Example 5
5 parts of a pomegranate methanol extract, 40 parts of ethanol, 1 part of lavender oil and 54 parts of water are thoroughly mixed with stirring to obtain an aqueous solution.
[0038]
Formulation Example 6
An aerosol container is filled with an ethanol solution in which 10 parts of a pomegranate methanol extract is dissolved to make a total of 50 parts. After attaching a valve to the container, 50 parts of a propellant (a 1: 1 mixture of Freon 11 and Freon 12) is pressure-filled through the valve part to obtain an aerosol agent.
[0039]
Formulation Example 7
An ethanol solution in which 2 parts of a pomegranate methanol extract is dissolved to make 20 parts as a whole and 80 parts of diatomaceous earth are mixed, thoroughly stirred and dried to obtain a powder.
[0040]
Test example 1
0.1 g of a culture medium (CE-2 manufactured by Nippon Claire Co., Ltd.), which was cultured for about 3 months, was killed by heat treatment. Next, the culture medium was added to a mixture of 250 mg of pomegranate methanol extract and 10 ml of 10% ethanol solution to obtain a test solution (hereinafter referred to as test solution a). The same procedure as described above was carried out except that 250 mg of pomegranate methanol extract was not included, and a test liquid (this is referred to as test liquid b) was obtained. The test solutions a and b were left for 3 hours and then filtered. 0.5 ml of the filtrate was mixed with 5 ml of 0.1 M phosphate buffer added so that TWEEN 20 was 0.05%, and the test solution treated with the allergen inactivating agent of the present invention (this solution) From the test solution b) and a test solution untreated with the allergen-inactivating agent of the present invention (referred to as test solution B). For test solutions A and B, the presence of mite allergens was examined by an ELISA method using a monoclonal antibody that specifically reacts with DerII, which is the major allergen of the leopard mite. As a result, the presence of the allergen was not confirmed in the test solution A, whereas the presence of the allergen was clearly confirmed in the test solution B.
[0041]
【Effect of the invention】
The present invention can provide an agent for inactivating allergens and a method for inactivating allergens using the agent.

Claims (3)

  An allergen inactivating agent comprising an extract of pomegranate leaves with a solvent consisting of at least one selected from water and a hydrophilic solvent.   2. The allergen inactivating agent according to claim 1, wherein the solvent comprising at least one selected from water and a hydrophilic solvent is an alcohol. A method for inactivating allergens, comprising treating an allergen-existing region using the allergen inactivating agent according to claim 1.