JP4958830B2 - Food composition containing peanut seed coat processed product as active ingredient - Google Patents

Description

  The present invention relates to a food composition containing a processed peanut seed coat as an active ingredient. More specifically, the present invention is a food composition used as a supplement for supplementarily supplying a functional ingredient into the body, preferably based on an excellent hyaluronidase inhibitory action, particularly an anti-aging effect, a skin beautifying effect, The present invention relates to a food composition capable of exerting an anti-inflammatory effect or an anti-allergic effect.

  In recent years, aging has attracted attention, and many researches have been vigorously advanced. The main cause of aging is aging, but specific examples of skin aging include decreases in mucopolysaccharides such as hyaluronic acid, cross-linking reaction of collagen, and cell damage due to ultraviolet rays. Can be mentioned.

  Hyaluronic acid is a linear polymer polysaccharide formed by alternately binding β-DN-acetylglucosamine and β-D-glucuronic acid. The hyaluronic acid is widely distributed in connective tissues of mammals, etc., and retains moisture in the cell gap, maintains elasticity, lubricity, flexibility and moisture retention, resistance to external forces such as mechanical failure, and bacterial infection. It has many functions such as prevention (Non-Patent Document 1). In addition, it is said that the amount of hyaluronic acid in the skin decreases with aging, and along with this, skin aging such as fine wrinkles and bulkiness appears. As a method for improving this, many cosmetics and foods containing hyaluronic acid have been proposed. However, there are enzymes in the living body that can break down hyaluronic acid (hyaluronidase) widely, including skin. Therefore, cosmetics and foods containing hyaluronic acid are expected to have anti-aging effects and beauty such as beautiful skin. The fact is that it is not effective. In particular, there is a report that the degradation rate of hyaluronic acid in the skin is fast and its half-life is about one day (Non-patent Document 2).

  Therefore, in order to obtain a higher anti-aging effect and skin beautifying effect, in addition to the method of actively supplying hyaluronic acid into the body or separately from the supply of hyaluronic acid, the activity of hyaluronidase in the body is suppressed. It is necessary to do it. That is, by suppressing the activity of hyaluronidase in the living body, hyaluronic acid that is inherently present in the living body can be stabilized, and decomposition of hyaluronic acid administered from the outside as a cosmetic or food can be suppressed. Beauty effects such as anti-aging or skin beautification of hyaluronic acid or hyaluronic acid-containing cosmetics and foods in the body can be made more effective.

  Hyaluronidase is also known as an inflammatory enzyme and an allergenic enzyme. Specifically, hyaluronidase is activated during inflammation, destroys the matrix of connective tissue, enhances infiltration of inflammatory tissues and vascular permeability, and hyaluronidase is a histamine from mast cells in type I allergy. It is known to mediate in the process of liberation. For this reason, by suppressing the activity of hyaluronidase, inflammation can be suppressed and allergic diseases such as hay fever and atopic dermatitis can also be improved.

  For these reasons, there is a demand for the development of a food that exhibits an excellent anti-aging effect, beauty effect such as beautiful skin, anti-inflammatory effect, or anti-allergic effect by adding a hyaluronidase inhibitor.

In addition, as naturally derived components having a hyaluronidase inhibitory action, for example, seeds such as grape seeds, hijiki, moroheiya, hamasuge, beans, rice and wheat, plants such as myrrh, perilla and hop or extracts thereof are known ( (See Patent Documents 1 to 7). It is also known that peanut seed coat extract has a hyaluronidase inhibitory action (see Patent Document 8).
Bio Industry, Vol. 8, p.346 (1991) J.Invest. Dermatol. Invest. Dermatol,, 97, 1260-130, 1991 JP 2000-26306 A JP 2002-293740 A JP 2003-238434 A JP 2003-137726 A JP 2003-252778 A JP 2005-263730 A JP 11-318387 A JP 2000-145757 A

  The present invention provides a food composition having an excellent hyaluronidase inhibitory action, specifically an anti-aging effect, a beautifying skin (especially moisturizing) effect, an anti-inflammatory effect, or an anti-allergic effect based on the excellent hyaluronidase inhibitory action An object of the present invention is to provide a food composition that can be used. In particular, an object of the present invention is to provide a food composition used as a supplement for supplementary supply into the body separately from a normal meal in anticipation of the above effects.

  The inventors of the present invention have been intensively studied to solve the above-mentioned problems, and used a combination of (1) peanut seed coat extract and (2) (a) glucosamine or a salt thereof, or (b) chondroitin sulfate. By doing so, it discovered that the hyaluronidase inhibitory activity which a peanut seed coat extract has increased remarkably. From this knowledge, the present inventors have an excellent hyaluronidase inhibitory activity by using the component (1) and the component (2) (component (a) or / and component (b)) in combination. The present invention was completed with the conviction that the food composition for the above purpose capable of exhibiting an anti-aging effect, a beautiful skin (particularly moisturizing) effect, an anti-inflammatory effect, or an anti-allergic effect can be provided.

  The present invention has been completed based on such findings, and includes the following aspects.

(I) food composition (I-1) (1) processed peanut seed coat, and (2) (a) glucosamine or a salt thereof, and (b) at least one selected from the group consisting of chondroitin sulfate Food composition.
(I-2) (1) A processed peanut seed coat and at least (2) (a) a food composition containing glucosamine or a salt thereof, (1) (2) to 1 part by weight of the processed peanut seed coat a) The food composition according to (I-1), wherein the ratio of glucosamine or a salt thereof is 100 to 1300 parts by weight.
(I-3) (1) A processed peanut seed coat and at least (2) (b) a chondroitin sulfate-containing food composition comprising (1) 1 part by weight of peanut seed coat (2) (b) The food composition according to (I-1), wherein the ratio of chondroitin sulfate is 1.2 to 1200 parts by weight.
(I-4) per day (1) at a rate such that the intake of processed peanut seed coat is 0.1 to 500 mg, (1) containing peanut seed coat processed (I-1) to (I- The food composition according to any one of 3).
(I-5) Per day (2) (a) Glucosamine or its salt intake in a total amount of 500 to 5000 mg, (b) chondroitin sulfate intake in a ratio of 50 to 2500 mg, (2) The food composition according to any one of (I-1) to (I-4), comprising (a) glucosamine or a salt thereof, and (b) at least one selected from the group consisting of chondroitin sulfate.

(I-6) (1) Processed peanut seed coat and (2) (b) a food composition containing chondroitin sulfate, (2) (b) containing a processed food product containing chondroitin sulfate (I-1) food composition characterized by the above-mentioned.
(I-7) A food composition comprising (1) a processed peanut seed coat and a food processed product containing at least (2) (b) chondroitin sulfate, (1) with respect to 1 part by weight of the processed peanut seed coat ( 2) (b) The food composition according to (I-6), wherein the ratio of the processed food product containing chondroitin sulfate is 1.2 to 1200 parts by weight in terms of the amount of chondroitin sulfate. .
(I-8) per day (2) (a) Glucosamine or its salt intake is 500-5000 mg in total, (b) Food processed food containing chondroitin sulfate converted to chondroitin sulfate (2) containing at least one selected from the group consisting of (a) glucosamine or a salt thereof, and (b) a processed food product containing chondroitin sulfate, in a ratio of 50 to 2500 mg, (I -6) or the food composition described in (I-7).
(I-9) The food composition according to any one of (I-6) to (I-8), wherein the processed food containing chondroitin sulfate is a processed shark cartilage.

(I-10) The food composition according to any one of (I-1) to (I-9), wherein the salt of glucosamine is glucosamine hydrochloride.
(I-11) The food composition according to any one of (I-1) to (I-10), which has a solid preparation form of tablets, pills, powders, granules, or capsules.
(I-12) The food composition according to any one of (I-1) to (I-11), which is a supplement.

  “Supplement” refers to nutrients (eg vitamins and minerals) that are often deficient in daily diet and functional ingredients that cannot be adequately supplemented in daily diet, separately from the normal diet. Such as tablets, pills, powders, granules, capsules (including soft capsules and hard capsules), liquid preparations such as drinks and syrups, etc. It means what has a different form of preparation from general food. In Japan, it is also called “dietary supplement” or “health supplement”, and in the United States it is also called “dietary supplement”. The “supplement” is a food having a purpose of health, health maintenance / promotion, etc. in a more positive sense than general food. Supplements targeted by the present invention include foods for specified health use (including conditional foods for specified health use) that can specifically show the function and effect of the food in a packaging container. Not limited to the description of specific functions and effects, foods that are labeled such that the functions and effects are imaged by consumers are also included.

(II) Method for enhancing hyaluronidase inhibitory activity of peanut seed coat processed product Also, as shown in the experimental examples described later, the inventors' research has shown that (a) glucosamine or a salt thereof, and (b It has been found that the hyaluronidase inhibitory activity of the processed peanut seed coat is remarkably enhanced by using in combination at least one selected from the group consisting of chondroitin sulfate. Therefore, the present invention also provides the following method based on such knowledge.

(II-1) (1) characterized in that the processed peanut seed coat is combined with at least one selected from the group consisting of (2) (a) glucosamine or a salt thereof, and (b) chondroitin sulfate, 1) A method for enhancing the hyaluronidase inhibitory activity of a processed peanut seed coat.
(II-2) (1) characterized in that (2) (a) glucosamine or a salt thereof is combined at a ratio of 100 to 1300 parts by weight with respect to 1 part by weight of the processed peanut seed coat (II-1) The method described in.
(II-3) (1) characterized by combining (2) (b) chondroitin sulfate at a ratio of 1.2 to 1200 parts by weight with respect to 1 part by weight of the processed peanut seed coat (II-1) The method described in.

(II-4) (1) A method for enhancing the hyaluronidase inhibitory activity of (1) groundnut seed coat processed product by combining (2) (b) chondroitin sulfate with (1) groundnut seed coat processed product, As described in (II-1), a processed food containing chondroitin sulfate is used.
(II-5) (1) For 1 part by weight of the processed peanut seed coat, (2) (b) 1.2 to 1200 parts by weight of the processed food containing chondroitin sulfate in terms of the amount of chondroitin sulfate The enhancing method according to (II-4), which is combined at a ratio.
(II-6) The method according to (II-4) or (II-5), wherein the processed food containing chondroitin sulfate is a processed shark cartilage.

  According to the present invention, a food composition having excellent hyaluronidase inhibitory activity, which has an anti-aging effect, a beautiful skin (particularly moisturizing) effect, an anti-inflammatory effect, or an anti-allergic effect based on the hyaluronidase inhibitory activity. Things can be provided.

  The food composition of the present invention is highly safe because it contains glucomin or a salt thereof having a dietary experience, chondroitin sulfate, particularly shark cartilage extract, and peanut seed coat used for food as an active ingredient, It can be effectively used as a supplement that is ingested daily as a supplement other than meals, particularly as a supplement having a solid preparation form.

(I) Food composition The food composition of the present invention is selected from the group consisting of (2) (a) glucosamine or a salt thereof and (b) chondroitin sulfate in addition to (1) processed peanut seed coat as an active ingredient It is characterized by including at least 1 sort combined.

(I-1) Peanut seed coat processed peanut (Arachis hypogaea L.) is one species of the order of the order of rose, legume, butterfly subfamily, Nanjing bean. Seeds are in a hard rice husk and covered with a thin seed coat, and are widely distributed for food. On the other hand, seed coats are not generally used for food, but may be used for food together with seeds.

  In the present invention, any known peanut seed coat can be used as the peanut seed coat. As the types of peanuts, for example, Tachi 1, improved Wadaoka, Tachi camel 1, Chiba Hanate, Chiba 55, Chiba 43, Chiba 74, Java 13, white oil 7-3, full grain, Valencia, 334A, Azuma handkerchief, Tecona, Waseda ryu, Benihandachi, Sachihomare, Tachimasari, etc. are known, but these peanut seed coats can be used without limitation.

  Peanut seed coats are usually dried and then subjected to various processing treatments (including pulverization, extraction, concentration, and drying (including spray-drying and freeze-drying)) according to the form of the present invention and the intended dosage form. The processed peanut seed coat of the present invention is simply referred to as “processed peanut seed coat”). In the present invention, those previously fermented or enzymatically treated can also be used.

  The peanut seed coat processed by the present invention includes, for example, a pulverized processed product (including both coarse powder and fine powder), an extract extracted with a solvent, a dried product thereof (dried extract), and a powder thereof. The powder dry extract extract made into can be mentioned. The extract is preferably an extract or a dried product thereof (dried extract or powder dry extract), and these are collectively referred to as “peanut seed coat extract”.

  In addition, the extract is a peanut seed coat, as it is or if necessary, dried, shredded, crushed, pressed or boiled, such as cold water, hot water or an organic solvent, or a mixture of water and an organic solvent, etc. It can be obtained by extracting with an extraction solvent. As an organic solvent used for this extraction, for example, methanol, ethanol, isopropanol, propylene glycol, 1,3-butylene glycol, acetone, ethyl acetate, chloroform, pentane, hexane, heptane, etc., alone or in combination of two or more kinds are used. Can be mentioned. Among the above extraction solvents, water; preferably lower alcohols (more preferably ethanol) such as methanol, ethanol and isopropanol, or a mixed solution of water and these lower alcohols.

  Moreover, as extraction temperature, it is preferable that it is room temperature or more, for example, it is more preferable to carry out at room temperature-about 200 degreeC.

  The method for preparing such an extract is not particularly limited, and can be performed according to a conventional method. As an example, 2 to 500 parts by weight, preferably 50 to 200 parts by weight of water or water-containing ethanol is added to 1 part by weight of the dried ground peanut seed coat, and room temperature to about 100 ° C, preferably about 50 to 80 ° C. An example is a method in which extraction is performed for about 1 to 300 minutes, preferably about 10 to 90 minutes with stirring, and then the solid content is removed by a solid-liquid separation method such as filtration or centrifugation.

  The extract thus obtained is a liquid material in which a soluble component (water-soluble component) of peanut seed coat is dissolved in an extraction solvent. Although it can be used as it is, it may be subjected to a purification treatment such as deodorization and decoloration as long as the effect is not affected. Moreover, the obtained extract may be prepared as a dried product (dry extract, powder dry extract). As a method of drying the extract to obtain a dry extract, a method of removing the extraction solvent by distillation under reduced pressure or the like, a method of removing the extraction solvent by performing a drying treatment such as vacuum drying or freeze drying, dry spray or spraying, etc. A drying process etc. can be mentioned. As the processed peanut seed coat, for example, a dried peanut seed coat is commercially available from Fukui Shoten Co., Ltd. and Fukuya Shoten Co., Ltd., and anyone can obtain it commercially.

  These peanut seed coats are proportions such that the daily intake of the food composition of the present invention is 0.1 to 500 mg, preferably 1 to 100 mg, more preferably 5 to 50 mg (equivalent to dry matter). It is desirable to be included. In addition, the ratio of the processed peanut seed coat contained in 100% by weight of the food composition of the present invention can be appropriately adjusted from the range of 0.2 to 20% by weight so as to be in the above range.

  The food composition of the present invention further contains (2) (a) glucosamine or a salt thereof, or (b) chondroitin sulfate, or both in addition to (1) the processed peanut seed coat. Thus, it can be prepared as a composition having higher hyaluronidase inhibitory activity (see Experimental Examples).

(I-2) Concomitant use with glucosamine or a salt thereof Glucosamine is a natural amino acid, and is a component widely distributed as a constituent component of mucopolysaccharide in cartilage, connective tissue and the like in an animal body. It is also abundant in crustacean chitin such as crabs and shrimps.

  In the present invention, glucosamine can be used as glucosamine itself, but preferably has a salt form from the viewpoint of absorbability. Examples of such salts include salts with inorganic acids such as glucosamine sulfate and glucosamine hydrochloride, and N-acetylglucosamine. Glucosamine hydrochloride is preferred. It is known that glucosamine having these salt forms is absorbed in the intestine after eating, and the salt is removed before entering the blood to become glucosamine and spread throughout the body.

  Glucosamine or a salt thereof can be usually prepared by extracting chitin from shrimp or crab shells, hydrolyzing it, and then purifying it. For example, glucosamine hydrochloride and sulfate are prepared from chitin obtained from crustaceans such as crabs and shrimps according to the methods described in JP-A No. 2004-359908, US Pat. No. 3,683,076, etc. -Acetylglucosamine can be similarly prepared according to the methods described in Japanese Patent Publication No. 5-33037 and Japanese Patent Application Laid-Open No. 2000-281696. Glucosamine derived from such shrimps and crabs or salts thereof are commercially available from, for example, Fuso Chemical Industry Co., Ltd., Saneigen FFI Co., Ltd., and Nakahara Co., Ltd., and anyone can obtain them commercially. However, glucosamine or a salt thereof is not necessarily purified as long as it can be ingested as a food. For example, a crude product prepared from shrimp or crab shells may be used.

  When the food composition of the present invention contains glucosamine or a salt thereof in addition to the processed peanut seed coat, the amount of the glucosamine or a salt thereof is the amount contained in the daily intake of the food composition of the present invention Is a total amount of 500 to 5000 mg, preferably 1000 to 3000 mg, and more preferably 1500 to 2000 mg (equivalent to a dried product).

  Moreover, as a compounding ratio with the groundnut seed coat processed material in a food composition, the ratio by which the hyaluronidase inhibitory activity of a groundnut seed coat processed material is enhanced by using both together can be mentioned. Specifically, as shown in the experimental examples described later, the total amount of glucosamine or a salt thereof is 20 to 3000 parts by weight, preferably 100 to 1300 parts by weight, more preferably 400 to 1 part by weight with respect to 1 part by weight of the processed peanut seed coat. The proportion of 1000, more preferably 400 to 750 parts by weight can be raised.

  In addition, the ratio (%) of the glucosamine or a salt thereof contained in 100% by weight of the food composition of the present invention is such that the daily intake is within the above range, and the blending ratio with the processed peanut seed coat is within the above range. It may be sufficient, and it can adjust suitably from the range of 10 to 99 weight%.

(I-3) Combined use with chondroitin sulfate Chondroitin sulfate is a kind of mucopolysaccharide discovered by Fischer et al. In cartilage tissue in 1861. D-glucuronic acid and N-acetyl-D-galactosamine disaccharide and sulfate residue It consists of Chondroitin sulfate is classified into types such as A, C, D, E, and K according to the binding position of the sulfate group. Chondroitin sulfate A is in the C-4 position of D-galactosamine, and chondroitin sulfate C is D- A sulfate group is bonded to the C-6 position of galactosamine. In addition, chondroitin sulfate D having a plurality of sulfate groups is in the C-6 position and D-2 glucuronic acid in the C-6 position of D-galactosamine, and chondroitin sulfate E is in the C-4 position and C-6 position in the D-galactosamine, Chondroitin sulfate K has sulfate groups bonded to the C-4 and C-6 positions of D-galactosamine and the C-2 position of D-glucuronic acid. Chondroitin sulfate B uses L-iduronic acid as a constituent sugar instead of D-glucuronic acid, and is also called deltaman sulfate. In general, higher vertebrates such as cattle and pigs have more chondroitin sulfate A, and lower vertebrates have more chondroitin sulfate C. In sharks often used as a raw material source, chondroitin sulfate is used for cartilage of fins, skulls, and middle bones C and D are abundant and chondroitin sulfate A is relatively small. Incidentally, chondroitin sulfate E is a characteristic component of squid cartilage, and chondroitin sulfate K is a characteristic component of horseshoe crab (FOOD Style 21, 2003, 11 (Vol. 7, No. 11)).

  Chondroitin sulfate can be prepared according to a method described in, for example, Japanese Patent Application Laid-Open No. 2000-273102 and Japanese Patent Application Laid-Open No. 2007-63400, using a connective tissue such as seafood, mammalian skin, trachea or cartilage as a raw material. Such chondroitin sulfate derived from a seafood, avian or mammalian connective tissue can be obtained from, for example, Toyo Fermentation Co., Ltd., Yaizu Suisan Chemical Co., Ltd., Adabtogen Pharmaceutical Co., Ltd. and the like.

  However, chondroitin sulfate is not necessarily purified as long as it can be ingested as a food, and may be a crude product prepared from, for example, the above-mentioned seafood, avian, or mammalian connective tissue. Such crudely purified products include processed food products obtained by processing a connective tissue of seafood, birds or mammals containing chondroitin sulfate as food.

  The processed food containing chondroitin sulfate can be preferably prepared from shark cartilage and fins, porcine cartilage and trachea, chicken cartilage, salmon and ray cartilage and the like.

  In order to be suitable for edible use, these are usually dried and then subjected to various processing treatments [pulverization treatment, extraction treatment, enzyme treatment, concentration treatment, drying treatment (spray-drying treatment, freezing treatment) according to the form and intended dosage form. (Including drying treatment), granulation treatment, etc.) and prepared as a processed food product.

  Examples of processed foods targeted by the present invention include, for example, dry pulverized processed products (including coarse powders and fine powders) processed so that the above raw materials can be taken as foods; organic solvents, water or these Examples include an extract extracted with a solvent such as a mixed solution; a dried product thereof (dried extract); and a powder dry extract extracted from the powder. Preferably, it is an extract or a dried product thereof (dry extract or powder dry extract).

  Examples of such processed foods include processed shark cartilage processed into food, preferably using shark cartilage as a raw material. More preferably, it is an extract of shark cartilage, or a dried product thereof (dried extract or powder dry extract). Hereinafter, this is also referred to as “shark cartilage extract”.

  Although it does not restrict | limit as a preparation method of a shark cartilage extract, For example, the method described in Unexamined-Japanese-Patent No. 2002-281935 can be illustrated. Specifically, after the shark cartilage raw material is washed with water, the shark cartilage extract is extracted by a method of extracting from the shark cartilage raw material with an alkaline solution, a method using a proteolytic enzyme, a method of extracting with hot water, or the like. The obtained shark cartilage extract is subjected to decolorization and deodorization using a solid adsorbent such as activated carbon, alumina, silica gel, activated clay, or the like, as it is or after enzymatic degradation using a protein hydrolase. Next, the decolorized and deodorized shark cartilage extract or its enzymatic degradation solution is treated with a separation membrane, preferably using an NF membrane with a salt rejection of 10 to 60%, so that the arsenic content is low, and the taste and smell are off. No shark cartilage extract can be obtained.

  The shark cartilage extract is commercially available from, for example, Yaizu Suisan Chemical Co., Ltd. and Toyo Fermentation Co., Ltd., and anyone can obtain it commercially. In addition to shark cartilage extract, pork cartilage extract is from Nippon Pure Food Co., Ltd., Nippon Biocon Co., Ltd., Shandong Yu (International) Trading Co., Ltd .; chicken cartilage extract is from Japan Biocon Co., Ltd .; The extract can be obtained commercially from Marukyo Bio Foods Co., Ltd .; salmon cartilage extract can be obtained commercially from C-Day Foods Co., Ltd. and Japan Barrier Free Co., Ltd.

  When the food composition of the present invention contains chondroitin sulfate in addition to the processed peanut seed coat, the amount of the chondroitin sulfate is 50 to 2500 mg contained in the daily intake of the food composition of the present invention. The ratio is preferably 100 to 2000 mg, and more preferably 250 to 1500 mg (equivalent to dry matter).

  Moreover, as a compounding ratio with the groundnut seed coat processed material in a food composition, the ratio by which the hyaluronidase inhibitory activity of a groundnut seed coat processed material is enhanced by using both together can be mentioned. Specifically, as shown in the experimental examples described below, chondroitin sulfate is 1.2 to 1200 parts by weight, preferably 3 to 300 parts by weight, more preferably 12 to 120 parts by weight, based on 1 part by weight of the processed peanut seed coat. Part, more preferably 12-60 parts by weight.

  Here, when using a crudely purified product, particularly a food processed product (preferably processed shark cartilage) containing chondroitin sulfate, instead of the purified chondroitin sulfate, it is converted from the amount of chondroitin sulfate contained therein. In the same manner as above, the blending ratio in the food composition and the blending ratio with respect to 1 part by weight of the processed peanut seed coat contained in the food composition can be set.

  For example, when using a food processed product containing chondroitin sulfate in a proportion of 60% by weight, the amount of the food processed product to the food composition of the present invention is the amount of the daily intake of the food composition of the present invention. The ratio of the contained amount is 85 to 4200 mg, preferably 170 to 3400 mg, more preferably 420 to 2500 mg (equivalent to dry matter). Moreover, it becomes 2-2000 weight part as a ratio of the said food processed material with respect to 1 weight part of peanut seed coat processed products, Preferably it becomes 5-500 weight part, More preferably, it becomes 20-200 weight part, More preferably, it becomes 20-100 weight part. You can increase the ratio.

  In addition, the ratio (%) of the chondroitin sulfate (or food processed product containing chondroitin sulfate) contained in 100% by weight of the food composition of the present invention is that the daily intake is within the above range and the processed peanut seed coat. What is necessary is just a mixture ratio to become the said range, and it can adjust suitably from the range of 10 to 99 weight%.

(I-4) Food Composition As described above, (1) processed peanut seed coat by using (2) (a) glucosamine or a salt thereof, or (b) chondroitin sulfate in combination with processed peanut seed coat Thus, a food composition having a significantly higher hyaluronidase inhibitory activity can be prepared. Therefore, the food composition of the present invention includes (1) processed peanut seed coat and (2) (a) glucosamine or a salt thereof (aspect 1), (1) processed peanut seed coat and (2) (b) containing chondroitin sulfate (Aspect 2), (1) processed peanut seed coat and (2) (a) glucosamine or a salt thereof, and (2) (b) containing chondroitin sulfate (Aspect 3) ) Is included.

  Even when the food composition of the present invention contains the above aspect 3, that is, (1) processed peanut seed coat, (2) (a) glucosamine or a salt thereof and (2) (b) chondroitin sulfate The blended amount and blending ratio of the processed peanut seed coat, (a) glucosamine or a salt thereof, and (b) chondroitin sulfate in the product can be set as described above.

  Specifically, the blended amount of peanut seed coat processed product, glucosamine or a salt thereof, and chondroitin sulfate in the food composition of the present invention is 0.1 to 500 mg, preferably 0.1 to 500 mg processed peanut seed coat per daily intake. 1 to 100 mg, more preferably 5 to 50 mg; glucosamine or a salt thereof (as a total amount) of 500 to 5000 mg, preferably 1000 to 3000 mg, more preferably 1500 to 2000 mg; chondroitin sulfate 50 to 2500 mg, preferably 100 to The ratio may be 2000 mg, and more preferably 250 to 1500 mg. Moreover, as a compounding ratio of each component, glucosamine or a salt thereof (as a total amount) is 20 to 3000 parts by weight, preferably 100 to 1300 parts by weight, and more preferably 400 to 1000 parts per 1 part by weight of the processed peanut seed coat. Parts by weight, more preferably 400 to 750 parts by weight; chondroitin sulfate 1.2 to 1200 parts by weight, preferably 3 to 300 parts by weight, more preferably 12 to 120 parts by weight, still more preferably 12 to 60 parts by weight Can be mentioned.

  Here, when using a crudely purified product, particularly a food processed product (preferably processed shark cartilage) containing chondroitin sulfate, instead of the purified chondroitin sulfate, it is converted from the amount of chondroitin sulfate contained therein. In the same manner as described above, the blending ratio in the food composition and the blending ratio with respect to 1 part by weight of the processed peanut seed coat can be set.

  The food composition of the present invention may be composed of the above-mentioned components, and it is used as an active ingredient, and other ingredients such as a pharmaceutically or food hygienically acceptable carrier or additive are blended. It may be. The type and amount of such a carrier or additive can be appropriately selected and adjusted according to the dosage form or intended use of the food composition of the present invention, as long as the effects of the present invention are not impaired.

  The food composition of the present invention exhibits a high hyaluronidase inhibitory activity by using (1) a processed peanut seed coat and (2) (a) glucosamine or a salt thereof, and / or (b) chondroitin sulfate. In addition, it is possible to suppress the in vivo degradation of hyalonic acid in the body and hyalonic acid administered from outside the body, and to stably obtain the effects of hyalonic acid (for example, cosmetic effects such as anti-aging and moisturizing). Moreover, the food composition of the present invention has an anti-inflammatory action and an anti-allergic action based on its high hyaluronidase inhibitory activity, and can be effectively used for improving hay fever, atopic disease, and the like.

  In addition to the above components, the food composition of the present invention can further contain other components such as a processed plant product, a functional component, or a medicinal component, as long as the effects of the present invention are not hindered. These components may be used alone or in combination with the above components, or two or more types may be arbitrarily combined and used in combination with the above components.

  As the processed plant product, as in the processed peanut seed coat used in the present invention, it has hyaluronidase inhibitory activity, for example, seeds such as grape seeds, hijiki, morohaya, hamasuge, beans, rice and wheat, and plants such as myrrh. Examples include extracts, basil, thyme and other herbs, cacao extract, coffee extract, plum extract, hawthorn extract, chlorella, etc. (see Patent Documents 1-7, etc.), processed willow (Japanese Patent Application No. 2007-71349) it can.

  Examples of functional components include humectants, antioxidants, hypoglycemic agents, anticholesterol agents, and immunostimulants.

  Here, examples of the humectant include hyaluronic acid or a salt thereof, collagen, ceramide, and betaine.

  Here, the antioxidant is not limited, but dry yeast, glutathione, lipoic acid, quercetin, catechin, coenzyme Q10, enzogenol, proanthocyanidins, anthocyanidins, anthocyanins, carotenes, lycopene, flavonoids, resaveratrol, isoflavones , Zinc, ginkgo leaves, moon peach leaves, hibiscus, melatonin. Among these, dry yeast is preferable because it contains a relatively large amount of glutathione known as an antioxidant and various minerals.

  Antihyperglycemic agents include, but are not limited to, indigestible dextrin, guava leaf, wheat albumin, L-arabinose, bean extract, mulberry leaf, ginger, salacia, α-linolenic acid, macaques, barley, ginseng, kidachi aloe, dandelion , Daidai, Korean thistle, garlic, pearl barley, banaba, bilberry, black cohosh, makomo, kotarahim, and tanaka.

  Anticholesterol agents include, but are not limited to, soy protein, phospholipid-bound soy peptide, chitosan, plant sterol ester, plant sterol, plant stanol ester, indigestible dextrin, sodium alginate, psyllium seed coat, astaxanthin, inositol, coenzyme A, Calcium, Magnesium, Carnitine, Silk Protein, Taurine, Methionine, α-Linolenic Acid, Gua Gum, Chondroitin Sulfate, Achacharu, Alfalfa, Ginkgo, Psyllium, Barley, Ginseng, Oats, Olives, Gadjus, Gymnema, Cat's Claw, Cuco, Chlorella , Spirulina, hawthorn, soy saponin, chili, garlic, bilberry, safflower, yucca, ruffa, agaricus, cordyceps, sockeye salmon, lei It can be mentioned.

  Examples of immunostimulants include agaricus, lactoferrin, cordyceps, arginine, tryptophan, valine, leucine, chitin, chitosan, aloe, kidachia aloe, echinacea, ogi, cat's claw, wolfberry, spirulina, pearl barley, safflower, maca, makomo, and ruma be able to.

  Examples of other medicinal ingredients include vitamins, amino acids, peptides, proteins, minerals (such as iron, zinc, magnesium and iodine), and fatty acids (such as EPA and DHA). Here, as vitamins, vitamins belonging to the vitamin A group (for example, retinal, retinol, retinoic acid, carotene, dehydroretinal, lycopene and pharmacologically acceptable salts thereof (for example, retinol acetate, retinol palmitate, etc.) Etc.), vitamins belonging to the group B (eg thiamine, thiamine disulfide, discetiamine, octothiamine, chicotiamine, bisbutiamine, bisbenchamine, prosultiamine, benfotiamine, fursultiamine, riboflavin, flavin adenine dinucleotide , Pyridoxine, pyridoxal, hydroxocobalamin, cyanocobalamin, methylcobalamin, deoxyadenocobalamin, folic acid, tetrahydrofolate, dihydrofolate, nicotinic acid, nicotinamide Nicotinic alcohol, pantothenic acid, panthenol, biotin, choline, inositol, pangamic acid and their pharmacologically acceptable salts thereof (eg thiamine hydrochloride, thiamine nitrate, dicetiamine hydrochloride, fursultiamine hydrochloride, riboflavin butyrate) Flavin adenine dinucleotide sodium, pyridoxine hydrochloride, pyridoxal phosphate phosphate, pyridoxal phosphate calcium, hydroxocobalamin hydrochloride, hydroxocobalamin acetate, calcium pantothenate, sodium pantothenate, etc.), vitamins belonging to the vitamin C group (ascorbic acid and its Derivatives, erythorbic acid and its derivatives and pharmacologically acceptable salts thereof (for example, sodium ascorbate, sodium erythorbate, etc.), vitamin Vitamins belonging to group D (for example, ergocalciferol, cholecalciferol, hydroxycholecalciferol, dihydroxycholecalciferol, dihydrotaxosterol and their pharmacologically acceptable salts), vitamin E group Vitamins belonging to (for example, tocopherol and derivatives thereof, ubiquinone derivatives and pharmacologically acceptable salts thereof (such as tocopherol acetate, tocopherol nicotinate, tocopherol succinate, tocopherol calcium succinate, etc.)), other vitamins (for example, Carnitine, ferulic acid, γ-oryzanol, orotic acid, rutin (vitamin P), eriocitrin, hesperidin and pharmacologically acceptable salts thereof (such as carnitine chloride). .

  As amino acids, leucine, isoleucine, valine, methionine, threonine, alanine, phenylalanine, tryptophan, lysine, glycine, asparagine, aspartic acid, serine, glutamine, glutamic acid, proline, tyrosine, cysteine, histidine, ornithine, hydroxyproline, hydroxy Examples include lysine, glycylglycine, aminoethylsulfonic acid (taurine), cystine, or pharmacologically acceptable salts thereof (for example, potassium aspartate, magnesium aspartate, cysteine hydrochloride, etc.). Preferably, branched chain amino acids such as valine, leucine and isoleucine, glutathione, cysteine, glutamic acid, glycine, serine, tryptophan, tyrosine, phenylalanine, histidine, methionine, threonine, lysine, cystine, arginine, alanine, aspartic acid, proline, amino Ethyl sulfonic acid.

  In the food composition of the present invention, the above-mentioned composition may be used as an oral composition as it is, and if necessary, tablets, pills, capsules (soft capsules, hard capsules), powders ( Powders), and various solid preparations such as granules (including dry syrup), or liquid preparations such as liquids for internal use (including liquids, suspensions, and syrups). it can. From the stability of each component, it is preferably a solid preparation form (dosage form).

  Formulation can be performed by a normal formulation method employed in the field of medicine and food (particularly supplements). For example, a tablet can be prepared by adding and blending each component according to the formulation, crushing, granulating, drying, sizing and mixing, and tableting the resulting preparation mixture.

  Furthermore, additives for formulation, for example, excipients, lubricants, binders, disintegrating agents, fluidizing agents, dispersing agents, wetting agents, preservatives, thickeners, pH adjustments as necessary An agent, a coloring agent, a flavoring agent, a surfactant, a solubilizing agent, and the like can be blended, and a coating tablet can also be formed using a coating agent. It can also be used as a paste glue.

  The estimated daily intake of the food composition of the present invention can be appropriately set and adjusted according to the age, sex, weight, and symptoms of the subject. For example, when targeting adults (weight 60 kg as a reference), the daily intake of processed peanut seed coat is 0.1 to 500 mg, preferably 1 to 100 mg, more preferably 5 to 50 mg (dry matter equivalent) ) Can be set as appropriate.

  When the food composition of the present invention contains (a) glucosamine or a salt thereof in addition to the processed peanut seed coat, in addition to the daily intake of the processed peanut seed coat being in the above range, a) It is desirable that the daily intake of glucosamine or a salt thereof is set to be in the range of 500 to 5000 mg, preferably 1000 to 3000 mg, more preferably 1500 to 2000 mg (equivalent to dry matter). When the food composition of the present invention contains (b) chondroitin sulfate, in addition to the daily intake of the processed peanut seed coat being in the above range, (b) the daily intake of chondroitin sulfate is 60 It is preferably set to be ˜2500 mg, preferably 100 to 2,000 mg, more preferably 250 to 1500 mg (equivalent to dry matter).

  In addition, the food composition of this invention can also be ingested by dividing this intake into 1 to several times a day.

(II) Method for Enhancing Hyaluronidase Inhibitory Activity of Peanut Seed Finished Product The enhancement of hyaluronidase inhibitory activity of peanut seed coat processed product is as follows: (1) Glucosamine or a salt thereof, (B) It can be carried out by using in combination at least one selected from the group consisting of chondroitin sulfate.

  Examples of the processed peanut seed coat (1) described above include those described in (I) above. Peanut seed coat extract is preferable.

  As described above, neither (a) glucosamine or a salt thereof nor (b) chondroitin sulfate need be a purified product, and includes (a) glucosamine or a salt thereof, or (b) chondroitin sulfate. Further, a crude product may be used as long as it is processed to be edible. (B) The crudely purified product of chondroitin sulfate is preferably a processed food obtained by processing seafood, avian or mammalian connective tissue containing chondroitin sulfate into food, and particularly preferably shark cartilage processed from shark cartilage. It is a cartilage processed product.

  (1) Used in combination with processed peanut seed coat (2) (a) Glucosamine or a salt thereof is as follows: (1) Processed peanut seed coat and (2) (a) Hyaluronidase inhibitory activity of glucosamine or a salt thereof In this range, the hyaluronidase inhibitory activities of each other can be enhanced without adversely affecting each other. For example, the ratio of (2) (a) glucosamine or a salt thereof to (1) 1 part by weight of processed peanut seed coat can be appropriately selected from the range of usually 20 to 3000 parts by weight. Preferably it is the range of 100-1300 weight part, More preferably, it is 400-1000 weight part, More preferably, it is the range of 400-750 weight part.

  The ratio of (2) (b) chondroitin sulfate used in combination with (1) processed peanut seed coat has an adverse effect on the hyaluronidase inhibitory activity of (1) processed peanut seed coat and (2) (b) chondroitin sulfate. It is the range which can enhance each other's hyaluronidase inhibitory activity by giving and using together. For example, the composition of (2) (b) chondroitin sulfate with respect to 1 part by weight of (1) groundnut seed coat processed product can be appropriately selected from the range of usually 1.2 to 1200 parts by weight. Preferably it is 3-300 weight part, More preferably, it is 12-120 weight part, More preferably, it is the range of 12-60 weight part. In place of the purified chondroitin sulfate, when using the above-described processed food containing chondroitin sulfate (preferably shark cartilage processed product), in terms of the amount of chondroitin sulfate contained therein, The formulation for 1 part by weight of the processed peanut seed coat can be set.

  According to the method of the present invention, (1) the peanut seed coat processed product is used in combination with at least one of (2) (a) glucosamine or a salt thereof, or (2) (b) chondroitin sulfate. (1) The hyaluronidase inhibitory activity of the groundnut seed coat processed product can be remarkably enhanced. Therefore, the present invention has a high hyaluronidase inhibitory activity, thereby suppressing the degradation of hyalonic acid in the body and hyalonic acid administered from outside the body, and the effects of hyalonic acid (for example, anti-aging, moisturizing and other cosmetics). A composition having a stable effect) and a composition having an anti-inflammatory action and an anti-allergic action based on high hyaluronidase inhibitory activity and can be effectively used for improving hay fever and atopy. And can be used effectively to provide.

  Hereinafter, the present invention will be described with reference to Preparation Examples, Examples and Experimental Examples, but the present invention is not limited to these Examples and the like.

  In addition, the glucosamine hydrochloride (powder) marketed from Wako Pure Chemical Industries Ltd. was used for the glucosamine hydrochloride used in the following Examples. As chondroitin sulfate, a shark cartilage extract (a commercially available shark cartilage extract (powder) having a chondroitin sulfate content of about 60% by weight), which is a food processed product containing chondroitin sulfate, was used. Moreover, the groundnut seed coat processed material was prepared by the method described in the following reference preparation examples.

Reference Preparation Example 1 Preparation of processed peanut seed coat 0.5 g of dry powder of peanut seed coat was put into 500 mL of purified water, and extracted with stirring for 30 minutes while heating at 60 to 70 ° C. Thereafter, the extract was filtered to recover the extract (1 mg / ml) (peanut seed coat extract). This is used as a processed peanut seed coat in the following experiment.

Experimental example 1
Various test samples were prepared by mixing the processed peanut seed coat (1 mg / ml) prepared in accordance with Reference Preparation Example 1 and glucosamine hydrochloride (powder) in the proportions described in Table 1 (Examples 1 to 5 and Comparative Examples). 1-6). About each prepared test sample, the hyaluronidase inhibitory effect was investigated according to the following method.

  Here, glucosamine hydrochloride used was obtained by dissolving 80 g of glucosamine hydrochloride (powder) in 500 mL of purified water (160 mg / ml).

(1) Hyaluronidase inhibitory action <Test method>
(1) 0.2 mL of the test sample prepared according to Table 1 and 0.2 mL of the hyaluronidase solution (0.6 mg of hyaluronidase dissolved in 1 mL of 0.1 M acetate solution) were mixed at 37 ° C. for 20 minutes. As a control test, the same test was performed except that 0.2 mL of 0.1 M acetate (control sample) was used instead of the test sample.

 (2) After the reaction, add 1.4 mL of hyaluronic acid solution (5 mg of hyaluronic acid dissolved in 1 mL of 0.1 M acetate solution) and 0.2 mL of 25 mM calcium chloride aqueous solution to the above mixed solution and mix for 40 minutes at 37 ° C. After the reaction, 20 μL of 4N sodium hydroxide solution was added to stop the reaction.

 (3) Add 50 μL of a solution prepared by mixing 6.1 g of dipotassium tetraborate tetrahydrate with 100 mL of purified water to 250 μL of the reaction stop solution obtained in (2), and react at 100 ° C. for 3 minutes. And then cooled to room temperature.

 (4) A solution prepared by adding 1.5 mL of purified water and 87.5 mL of glacial acetic acid to 11 mL of concentrated hydrochloric acid and adding 90 mL of glacial acetic acid to 10 mL of a solution of 10 g of 4 (N, N-dimethylamino) -benzaldehyde in advance. Then, 2.5 mL was added to the reaction solution obtained in (3) above, reacted at 37 ° C. for 20 minutes, and then centrifuged at 2000 rpm for 2 minutes.

 (5) Transfer 200 μL of the reaction solution obtained in (4) above to a 96-well plate and measure the absorbance at a measurement wavelength of 585 nm using a plate reader (product name: GENIOS Spectra FLUOR plus, manufacturer: TECAN). Then, the inhibition rate (%) of hyaluronidase activity was measured.

<Result>
The results are shown in Table 1.

  In addition, Table 1 shows the hyaluronidase activity inhibition rate (%) (measured value) measured for each test sample of Examples 1 to 5 and Comparative Examples 1 to 6, and in order to evaluate the influence of the combination of the two types, glucosamine hydrochloride The theoretical value computed from each hyaluronidase activity inhibition rate (%) (measured value) of salt single goods (Comparative Examples 1-5) and peanut seed coat processed goods single article (Comparative Example 6) is shown.

  From the hyaluronidase activity inhibition rate (%) (measured value) measured for each test sample of Examples 1 to 5, the substantial effect was evaluated according to the following criteria. In addition, the ratio between the measured value and the theoretical value [(measured value / theoretical value) × 100) was determined, and the synergistic effect of the combined use of the two kinds was evaluated based on the following criteria.

  The experimental results shown in Table 1 above are that peanut seed coats and glucosamine hydrochloride are used in combination, and in particular, glucosamine hydrochloride is used in a ratio of 100 to 1300 parts by weight with respect to 1 part by weight of peanut seed coats. This shows that the hyaluronidase inhibitory activity is enhanced (Examples 1 to 5). In particular, it has been found that the enhancement effect is enhanced by the combined use of 400 to 1000 parts by weight, preferably 400 to 750 parts by weight, of glucosamine hydrochloride with respect to 1 part by weight of the processed peanut seed coat.

Experimental example 2
Test samples prepared by mixing peanut seed coat and chondroitin sulfate in the proportions shown in Table 2 were examined for hyaluronidase inhibitory activity in the same manner as in Experimental Example 1. Here, the blending amount of chondroitin sulfate was calculated from the chondroitin sulfate content (about 60% by weight) in the shark cartilage extract used as the chondroitin sulfate raw material. The shark cartilage extract was prepared by dissolving 5 g of shark cartilage extract (powder) in 500 mL of purified water (10 mg / ml, 6 mg / ml in terms of the amount of chondroitin sulfate). In addition, two types of processed peanut seed coats were used: peanut seed coat extract (1 mg / ml) and further diluted 10 times with purified water (0.1 mg / ml).

<Result>
The results are shown in Tables 3 and 4.

  Table 3 shows the hyaluronidase activity inhibition rate (%) (measured value) measured for each of the test samples of Examples 11 to 15 and Comparative Examples 11 to 16, and chondroitin sulfate in order to evaluate the influence of the combination of the two types. It shows the theoretical value calculated from the hyaluronidase activity inhibition rate (%) derived from the peanut (Comparative Examples 11-15) and the hyaluronidase activity inhibition rate (%) derived from the processed peanut seed coat (Comparative Example 16).

  In addition, Table 4 shows the hyaluronidase activity inhibition rate (%) (measured value) measured for each test sample of Examples 21 to 24 and Comparative Examples 21 to 25, and in order to evaluate the influence of the combination of the two, chondroitin sulfate It shows the theoretical value calculated from the hyaluronidase activity inhibition rate (%) derived from the peanut (Comparative Examples 21 to 24) and the hyaluronidase activity inhibition rate (%) derived from the processed peanut seed coat (Comparative Example 25).

  The experimental results shown in Tables 3 and 4 show that the processed peanut seed coat and chondroitin sulfate are used in combination, and particularly chondroitin sulfate is used in a ratio of 1.2 to 300 parts by weight per 1 part by weight of the processed peanut seed coat. This indicates that the hyaluronidase inhibitory activity is enhanced. In particular, it has been found that the enhancement effect is enhanced by the combined use of 3 to 300 parts by weight, preferably the combined use of 3 to 120 parts by weight, more preferably 12 to 120 parts by weight, particularly preferably 12 to 60 parts by weight. (Examples 11-15, 21-24).

Formulation Examples 1-29
Compositions (formulation examples 1 to 29) composed of the formulations described in Table 5 were prepared as tablets according to a conventional method. Specifically, each component was blended according to the formulation, pulverized, granulated, dried, sized and mixed, and tableted according to a conventional method to prepare a tablet.

Formulation examples 30-59
Compositions (formulation examples 30 to 58) having the formulation described in Table 6 were prepared as granules according to a conventional method. Specifically, each component was blended according to the formulation, and mixed, granulated, dried and sized according to a conventional method to prepare granules.

Claims (7)

(1) Processed peanut seed coat, and (2) a food composition containing at least one selected from the group consisting of (a) glucosamine or a salt thereof, and (b) chondroitin sulfate ,
(1) to 1 part by weight of processed peanut seed coat (2) (a) 20 to 1300 parts by weight in total of glucosamine or salt thereof, and / or (1) to 1 part by weight processed peanut seed coat (2) ( b) A food composition characterized in that the ratio of chondroitin sulfate is 1.2 to 1200 parts by weight . (1) A processed peanut seed coat and a food composition containing at least (2) (a) glucosamine or a salt thereof,
(1) The ratio of (2) (a) glucosamine or a salt thereof to 1 part by weight of the processed peanut seed coat is 20 to 1300 parts by weight in total, The food composition according to claim 1. (1) A peanut seed coat processed product and at least (2) (b) a chondroitin sulfate-containing food composition,
The ratio of (2) (b) chondroitin sulfate to 1 part by weight of (1) groundnut seed coat processed product is 1.2 to 1200 parts by weight, The food composition according to claim 1. The food composition according to any one of claims 1 to 3, comprising (1) the processed peanut seed coat at a rate such that the daily intake of (1) the processed peanut seed coat is 0.1 to 500 mg. .   (2) (a) Glucosamine or salt at a rate such that (2) (a) glucosamine or its salt intake is 500-5000 mg in total and (b) chondroitin sulfate intake is 50-2500 mg per day The food composition according to any one of claims 1 to 4, comprising at least one selected from the group consisting of a salt thereof and (b) chondroitin sulfate.   The food composition according to any one of claims 1 to 5, which has a solid preparation form of a tablet, pill, powder, granule or capsule.   The food composition according to any one of claims 1 to 6, which is a supplement.