JP5280672B2 - Beta-glucan peptide derivatives exhibiting action to eliminate harmful substances - Google Patents

Description

The present invention relates to a beta-glucan peptide derivative exhibiting a toxic substance elimination action having an oxidation-reduction potential of minus 1 mV to minus 500 mV, and a method for producing the same.

The pollution of rivers, lakes, and oceans is becoming more and more serious year by year, leading to massive environmental destruction. The Japanese Environment Agency, European Environment Agency and the US Department of the Environment (EPA) are trying to improve polluted water flowing into rivers, lakes and oceans in order to improve the global environment.

In addition, air pollution due to mass consumption of fossil fuels is also serious, and an increase in carbon dioxide emissions has been observed on a global scale and is reported to be related to global warming. At the same time, there are many fine particles such as diesel particles and soot in the atmosphere, which absorb sunlight and cause heat to float in the atmosphere, which warms the atmosphere and is related to global warming. It is presumed that

In the living environment, there are an increasing number of patients who cause hypersensitivity and respiratory distress due to indoor chemical substances such as formaldehyde and formalin such as sick house syndrome and sick building syndrome, and biological harmful substances such as minute diesel particles and house dust mites.

As countermeasures against these harmful substances, although a dust prevention mask, an intake filter and a dust suction machine are being used, a complete solution has not been achieved.

As an invention relating to the improvement of harmful substances, there is an invention of a redox material such as water and soil, which is characterized in that it is mainly fermented by aerobic bacteria using seed shells such as rice husk and rice bran Although redox materials such as water and soil have been reported, the main useful components and substances have not been identified (for example, see Patent Document 1).

In addition, as water purification agents, inventions of water purification agents containing live, dead cells or products of microorganisms classified as Enterococcus faecalis are recognized, but the microorganisms used are harmful. The practical use is limited (for example, see Patent Document 2).

Furthermore, there is an invention of a water quality / soil improving agent, a method for producing the same, and a method for administering the same, and it is obtained by washing natto with water and transferring the natto bacteria, the viscous part of the natto surface and the easily degradable product of the natto surface into the wash water. Although the soil quality improving agent is explained, the effect is weak and its industrial use is limited (for example, refer to Patent Document 3).

There is an invention related to water quality improvement and malodor control of sewage treatment plants by liquid lactic acid bacteria metabolites, here it is a liquid substance mixed in the inflow tank of the sewage treatment plant and improves the bacterial flora involved in organic matter contained in sewage Although water quality improvement and malodor control materials have been described, their use in industry is limited (for example, see Patent Document 4).

On the other hand, the beta glucan peptide derivative exhibiting a specific structure is the present invention regarding a production method characterized by the structural characteristics of the binding of tripeptide, beta glucan and lactic acid, and the production characteristics of fermentation and reduction treatment. This will be described below.
JP2006-312145 JP 2005-270735 A JP-A-2005-230593 JP-A-11-47779

As described above, although various methods and products have been developed for excretion of harmful substances, there are problems that the effect is not clear and the use in industry is not clear.

The present invention has been made paying attention to the problems existing in the prior art as described above. The object is to provide a beta-glucan peptide derivative that has low side effects and exhibits an excellent toxic substance-erasing action.

The beta which exhibits the hazardous | toxic substance elimination effect | action of Claim 1 characterized by adding the red koji mold | fungi, natto bacteria, baker's yeast, and lactic acid bacteria to rice bran and soybean powder, and carrying out the alkali reduction of the fermented liquid which carried out mixed fermentation. It is to provide an efficient production method of a glucan peptide derivative.

In order to achieve the above object, the invention according to claim 1 relates to a beta-glucan peptide derivative having a redox potential of minus 1 mV to minus 500 mV and exhibiting a harmful substance elimination action represented by the following formula (1). It is.

Since this invention is comprised as mentioned above, there exist the following effects.

According to the beta glucan peptide derivative according to claim 1, the side effect is weak and an excellent toxic substance eliminating action is exhibited.

Hereinafter, embodiments embodying the present invention will be described in detail.

First, a beta-glucan peptide derivative exhibiting a harmful substance elimination action represented by the following formula (1) having a redox potential of minus 1 mV to minus 500 mV will be described.

The beta glucan peptide derivative here is a tripeptide composed of isoleucyl-cysteinyl-cysteine linked to three beta 1-3 linked glucoses with an ester bond to the hydroxyl group at the 4-position of glucose. Shows the basic structure in which an ester bond is formed.

Lactic acid has an ester bond with the carboxyl group of lactic acid and the hydroxyl group at the 1-position of glucan.

The beta glucan peptide derivative represented by the above formula (1) has an SH group derived from two cysteines in the tripeptide. These constituting amino acids are all in the L form.

This beta-glucan peptide derivative has a binding number of 3 as beta-glucan and is a short chain, but it has the properties of beta-glucan and possesses the function of regulating immune action, and has sick house syndrome, chemical substances and atmospheric It is also effective against allergies and airway stenosis caused by contamination.

The beta glucan part of the beta glucan peptide derivative has a cyclic structure and encloses fine particles such as diesel particles and asbestos. It has the feature that harmful particles can be adsorbed and detoxified.

In the tripeptide portion of this beta glucan peptide derivative, the two reduced SH groups exhibit a strong reducing action, and when the aqueous solution is about 1 mg / 100 ml, the redox potential of the solution exhibits minus 1 mV to minus 500 mV.

Harmful substances disappear by utilizing the reducing action of reactive aldehydes such as formalin, halogen substances such as chloroform, oxidizing substances, cyanide compounds and diesel particles.

Further, volatile gases such as carbon dioxide, carbon monoxide, nitrogen oxide, sulfur dioxide, hydrogen sulfide, and ammonia are also eliminated through the above reducing action.

Furthermore, for toxic metals such as arsenic, lead, mercury, and cadmium, the two SH groups exhibit chelating action, capture the toxic metal, and fold the glucan ring structure so that the toxic metal is folded. Envelop and disappear.

In addition, the lactic acid part has the function of distinguishing harmful substances and useful components for the living body. Trace elements and minerals necessary for the living body are not recognized by lactic acid and do not react with this substance. Useful trace components and vitamins are not impaired and are safe.

In addition, when this beta-glucan peptide derivative is ingested in excess, it is degraded by esterases in the digestive tract, lungs, blood, and organs to be decomposed into tripeptides, beta-glucan and lactic acid. Tripeptides become amino acids, and are further decomposed into sulfur dioxide and carbon dioxide gas and excreted from the kidney, so that the safety is high and more preferable.

This beta glucan peptide derivative is preferable against various inflammations in the whole body because it exhibits an anti-inflammatory action and also suppresses inflammation caused by harmful substances.

In addition, this beta-glucan peptide derivative enhances skin regenerative power by promoting detachment of skin cells from keratinocytes, and further, atopy and contact allergy caused by harmful substances through anti-inflammatory action. It is preferable because of improving.

Furthermore, since this beta glucan peptide derivative has a redox potential of minus 1 mV to minus 500 mV, it has a strong reducing action, assisting the action of vitamin C, decomposing melanin as an oxidation product, and causing stains. Eliminates substances and promotes collagen production.

This oxidation-reduction potential of minus 1 mV to minus 500 mV is generated by alkali reduction or an ion reduction device. On the other hand, when the oxidation-reduction potential is positive, there is a possibility that the tissue may be damaged by oxidation. On the other hand, it is preferable that the oxidation-reduction potential is negative from the viewpoint of suppressing oxidation and protecting the tissue.

Since this beta glucan peptide derivative has a negative redox potential, it reduces oxidative stress such as skin oxidation and ultraviolet rays.

This beta glucan peptide derivative is obtained by fermentation with plant cells, animal cells, yeasts or microorganisms, and can be obtained by biosynthesis.

This beta glucan peptide derivative is obtained as a liquid or powder, and can be used as a water quality improver, air freshener, pharmaceutical material, food material, cosmetic material, daily necessities, and soil improver.

As a water quality improver, it is used as a river or ocean environment improver for the purpose of removing heavy metals in water. In addition, it is used as a bath cleaning agent and a drainage detergent for the purpose of removing spoilage microorganisms, oxidizing substances, heavy metals, toxic substances and ammonia odor adhering to drainage grooves and pipes.

It is used for the purpose of removing ammonia and harmful substances by using it as a household laundry detergent, dishwashing detergent and bath detergent. It can be used as a cleaning agent for sink tanks, sinks, bathhouses, cars and houses for the purpose of removing harmful substances.

It can also be used as a toilet cleaner, for the purpose of removing harmful substances, and as a deodorant that uses the deodorizing effect for removing ammonia and stool odors.

As an air cleaner, it is used as a cleaner, a fragrance or a spray for the purpose of removing formalin, ammonia, fine particles and halogen compounds by a beta glucan peptide derivative.

When used as a pharmaceutical material, it is preferable to separate and purify the target beta-glucan peptide derivative because the purity of the target beta-glucan peptide derivative is increased and impurities can be removed.

Used as pharmaceuticals, parenteral preparations such as injections, oral preparations and coatings, and quasi-drugs used in tablets, capsules, drinks, soaps, coatings, gels, toothpastes, etc. Is done.

Examples of oral preparations include tablets, capsules, powders, syrups, and drinks. When mixed with the above-mentioned tablets and capsules, it can be used together with a binder, excipient, swelling agent, lubricant, sweetener, flavoring agent and the like. The tablets can also be coated with shellac or sugar.

Moreover, in the case of the said capsule, liquid carriers, such as fats and oils, can be further contained in said material. In the case of the above syrup and drink, sweeteners, preservatives, pigment flavoring agents and the like can be contained.

Examples of parenteral preparations include injections in addition to external preparations such as ointments, creams, and liquids. Vaseline, paraffin, fats and oils, lanolin, macro gold, etc. are used as a base material for external preparations, and can be made into ointments, creams, and the like by ordinary methods.

Injections include liquids, and other lyophilization agents. This is used aseptically dissolved in distilled water for injection or physiological saline at the time of use.

Food preparations are used as raw materials for raw material processing and food production for the purpose of removing harmful substances, and as spraying agents for the purpose of hygiene management in food factories. It is preferable to use it for food.

When the obtained food preparation is used for pets and livestock animals such as dogs and cats, it is used as a detergent or feed for the purpose of removing harmful substances in the feed, absorbing fecal odor, and antibacterial action.

It can be used together with surfactants, solvents, thickeners, excipients and the like according to conventional methods as cosmetics. For example, it can be in the form of oil-soluble cream, hair gel, facial cleanser, beauty essence, lotion and the like. The form of the cosmetic is arbitrary, and can be used as a solution, cream, paste, gel, gel, solid or powder.

The resulting cosmetic removes harmful substances that cause hypersensitivity, thus promoting keratin improvement and skin regeneration in atopic patients. Moreover, since it exhibits a reducing action and a whitening action, it is also used as a whitening cosmetic or a quasi-drug.

As a soil conditioner, it is used on farms for the purpose of removing heavy metals in fields and fields. It is also used in chemical factories for the purpose of improving the surrounding soil contamination and working environment.

The beta glucan peptide derivative thus obtained is obtained as a liquid or a powder.

Hereinafter, the embodiment will be specifically described with reference to examples and test examples.

Rice bran obtained from Koshihikari rice ears grown in Gifu Prefecture under pesticide-free and organic conditions was used. This was washed with water and then pulverized with a pulverizer (Super Free Mill manufactured by Nara Machinery Co., Ltd.) to obtain 1 kg of rice bran pulverized product.

Soybeans from Hokkaido were used in a mixer (Cuisinate) to obtain 1 kg of soybean pulverized product. These were subjected to autoclaving and sterilized at 121 ° C. for 20 minutes. 1 kg of each was put into a clean fermentation tank (sterilized round 40 liter tank for fermentation), and 10 kg of sterilized tap water was added and stirred.

Separately, 10 g of powdered red koji mold (manufactured by Kurisu Honpo) was used in a small fermentation tank, and a sterilized soybean powder and a precultured culture solution were prepared.

Moreover, 10 g of powdered natto bacteria (manufactured by Natto Motopo) were used in a small fermentation tank, and a sterilized soybean powder and a precultured culture solution were prepared.

Furthermore, 10 g of baker's yeast made of oriental yeast was used in a small fermentation tank, and a culture solution pre-cultured with sterilized flour was prepared.

A lactic acid bacterium Lactobacillus casei 10 g obtained from Biofermin Pharmaceutical was used in a small fermentation tank to prepare a sterilized soybean powder and a precultured culture solution.

The pre-cultured red yeast, natto, baker's yeast, and lactic acid bacteria solution was added to the fermentation tank containing the rice bran and soybeans, and after stirring, heated in a temperature range of 40 to 45 ° C. and fermented. .

In the fermentation process, the fermentation broth was sampled while bubbling and stirring by aeration, and the production of the target beta glucan peptide derivative was measured.

In the method, the fermented liquid was subjected to high performance liquid chromatography with a mass spectrometer (HPLC, Shimadzu Corporation) and analyzed to confirm the production of the target substance.

As a result, a sufficient amount of the desired beta glucan peptide derivative was produced 10 days after the fermentation, and thus the fermentation was terminated. After stopping the fermentation, 5 kg of ethanol at 30 ° C. was added to the fermentation broth.

This fermentation broth was subjected to a filter with diatomaceous earth and filtered. The obtained filtrate was used for Cellar Kiss (manufactured by Zenon Co., Ltd.).

The obtained filtrate was subjected to alkali reduction by subjecting to an alkali reduction device (alkaline reduced water / strongly oxidized water continuous generator “Protech ATX-501” manufactured by Zemaitis).

By carrying out alkali reduction in this manner, the beta glucan peptide derivative exhibited a redox potential of minus 1 mV to minus 500 mV when suspended in an aqueous solution or water.

The alkali-reduced product thus obtained was subjected to a lyophilizer manufactured by FP Japan, and 230 g of the intended beta glucan peptide derivative was obtained as a powder. This was used as a specimen 1 for the following test.

Below, the test method and result regarding the structural analysis of a beta glucan peptide derivative are demonstrated.
(Test Example 1)

The specimen 1 obtained as described above was dissolved in an extraction medium, analyzed by high performance liquid chromatography with a mass spectrometer (HPLC, Shimadzu Corporation), and further with a nuclear magnetic resonance apparatus (NMR, Bruker, AC-250). Analyzed. As a result of structural analysis, a beta-glucan peptide derivative bound with the target tripeptide was identified from specimen 1.

The oxidation-reduction potential test is described below.
(Test Example 2)

0.1 g of the sample 1 was dissolved in 100 ml of tap water (Tokyo). The oxidation-reduction potential was measured with an oxidation-reduction potentiometer (manufactured by Sato Corporation, OPR Pro).

As a result, the oxidation-reduction potential of specimen 1 one minute after lysis was minus 136 mV, and the minus potential was maintained until 12 hours after lysis. On the other hand, the redox potential of tap water was plus 324 mV.

The heavy metal adsorption and cyan adsorption tests are described below.
(Test Example 3)
As heavy metals, mercury chloride (Wako Pure Chemical), arsenic (Tokyo Chemical), lead chloride (Wako Pure Chemical), cadmium chloride (Tokyo Chemical), organic cyanide (potassium cyanide) (Wako Pure Chemical), pesticide (acetoate) Kaketakeda Horticulture Co., Ltd.) and pyrimifosmethyl (Sumika Takeda Horticulture Co., Ltd.) were used.

These were dissolved in dimethyl sulfoxide to prepare liquids containing 100 ppm each.

0.01 mg of the beta glucan peptide derivative obtained in Sample 1 was added thereto and left for 1 hour. This solution was subjected to ultrafiltration and separated into a beta glucan peptide derivative component (not filtered) and a filtrate. Mercury chloride, arsenic, lead, and cadmium contents of the filtrate were measured by an atomic absorption measuring device (Shimadzu Corporation, AA-6800).

The cyanide content of the filtrate was measured by the HPLC method using a Ponal kit CN · T manufactured by Dojin Chemical.

As a result, the mercury chloride, arsenic, lead and cadmium contents of the filtrate were 1 ppm, 2 ppm, 1 ppm and 1 ppm, respectively. The cyan content of the filtrate was 1 ppm. Furthermore, the concentrations of acephate and pyrimifosmethyl were 0 ppm and 1 ppm, respectively.

As a result, it was found that the beta glucan peptide derivative of Sample 1 adsorbs and eliminates mercury chloride, arsenic, lead, cadmium, cyanide and pesticides.

The formaldehyde, chloroform and ammonia adsorption tests are described below.
(Test Example 4)
1 g each of formaldehyde (manufactured by Wako Pure Chemical Industries), chloroform (manufactured by Wako Pure Chemical Industries) and ammonia (manufactured by Wako Pure Chemical Industries) was dissolved in 100 ml of water to prepare a formaldehyde solution, a chloroform solution and an ammonia solution, and placed in an airtight container. .

0.01 mg of the beta glucan peptide derivative obtained in Sample 1 was added to each solution and allowed to stand for 1 hour.

The concentration of formaldehyde, chloroform and ammonia in the air in the sealed container was measured with a suction type gas measuring device.

As a result, when the specimen 1 was not added, the concentrations of formaldehyde, chloroform and ammonia in the air in the sealed container were 236 ppm, 156 ppm and 243 ppm, respectively.

On the other hand, in the case of 1 hour after adding the specimen 1, the concentrations of formaldehyde, chloroform and ammonia in the air in the sealed container were 1 ppm, 0 ppm and 2 ppm, respectively.

As a result, it was found that Sample 1 erases formaldehyde, chloroform and ammonia and suppresses release into the air.

The beta-glucan peptide derivative obtained in the present invention exhibits excellent adsorption and elimination action against heavy metal pollution, water pollution and soil pollution due to cyanide, and an improvement action against the pollution is recognized. Therefore, environmental protection regarding water pollution and soil pollution is observed. Contribute to.

According to the beta-glucan peptide derivative obtained in the present invention, excellent treatment effects and preventive action are recognized for indoor contamination with formaldehyde, chloroform, ammonia, sick house syndrome, asthma patients and atopic patients, and the quality of living environment is improved. Contribute to.

Claims (1)

A beta-glucan peptide derivative exhibiting a toxic substance-erasing action represented by the following formula (1) having a redox potential of minus 1 mV to minus 500 mV.